##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936654_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1094796 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48016616794362 31.0 31.0 34.0 30.0 34.0 2 31.71740123274108 31.0 31.0 34.0 30.0 34.0 3 31.98031140048009 33.0 31.0 34.0 30.0 34.0 4 35.802098290457764 37.0 35.0 37.0 35.0 37.0 5 35.623887920671976 37.0 35.0 37.0 33.0 37.0 6 35.742048746981176 37.0 35.0 37.0 33.0 37.0 7 35.67658632293139 37.0 35.0 37.0 33.0 37.0 8 35.65595508204268 37.0 35.0 37.0 33.0 37.0 9 37.28017822498438 39.0 37.0 39.0 34.0 39.0 10-11 37.170576527499186 39.0 37.0 39.0 33.0 39.0 12-13 37.05305920007015 39.0 37.0 39.0 33.0 39.0 14-15 38.145184125627054 40.0 37.0 41.0 33.0 41.0 16-17 38.077292481887035 40.0 37.0 41.0 33.0 41.0 18-19 38.18626027131995 40.0 37.5 41.0 33.5 41.0 20-21 38.25637516030384 40.0 38.0 41.0 33.5 41.0 22-23 38.16217952933697 40.0 37.5 41.0 33.0 41.0 24-25 38.044166219094706 40.0 37.0 41.0 33.0 41.0 26-27 37.815268323961725 40.0 37.0 41.0 33.0 41.0 28-29 37.72458110917468 40.0 37.0 41.0 32.5 41.0 30-31 37.65166889539238 39.5 37.0 41.0 33.0 41.0 32-33 37.42191650316589 39.0 36.0 41.0 32.0 41.0 34-35 37.239284761727305 39.0 36.0 41.0 32.0 41.0 36-37 36.97262686381755 39.0 35.0 41.0 31.0 41.0 38-39 36.76275534437466 39.0 35.0 41.0 31.0 41.0 40-41 36.489960230033724 39.0 35.0 41.0 30.0 41.0 42-43 36.18792085466151 39.0 35.0 40.0 30.0 41.0 44-45 35.81991119806795 39.0 35.0 40.0 29.5 41.0 46-47 35.30712571109138 38.0 35.0 40.0 26.5 41.0 48-49 34.74448436055667 38.0 34.0 40.0 24.5 41.0 50-51 34.51032384115397 38.0 34.0 40.0 24.0 41.0 52-53 34.22490400037998 38.0 33.5 40.0 23.0 41.0 54-55 33.943246961077676 38.0 33.0 40.0 23.0 41.0 56-57 33.65830666169771 37.5 33.0 40.0 22.0 41.0 58-59 33.28298514061068 37.0 33.0 40.0 20.5 41.0 60-61 32.899532424305534 36.5 32.0 39.5 19.0 41.0 62-63 32.59636909524697 36.0 32.0 39.0 18.0 40.0 64-65 32.15030836795165 35.5 31.0 39.0 16.5 40.0 66-67 31.75373448569414 35.0 31.0 38.5 13.5 40.0 68-69 31.28650314761837 35.0 30.5 38.0 9.5 40.0 70-71 30.815064633045793 34.5 30.0 37.0 7.5 39.5 72-73 30.412200537817093 34.0 30.0 37.0 7.0 39.0 74-75 30.058800909027802 34.0 29.0 36.0 4.0 39.0 76-77 28.64776040467813 32.5 28.0 35.0 2.0 37.0 78-79 29.432052638117057 34.0 29.0 35.0 2.0 37.0 80-81 29.291554773674733 34.0 29.0 35.0 2.0 37.0 82-83 28.948321879144608 34.0 29.0 35.0 2.0 36.5 84-85 28.510356267286326 34.0 29.0 35.0 2.0 36.0 86-87 28.155144885439846 33.5 27.5 35.0 2.0 36.0 88-89 27.732808212671586 33.0 27.0 35.0 2.0 35.0 90-91 27.29478733937647 33.0 26.0 35.0 2.0 35.0 92-93 26.84875036079781 33.0 25.0 35.0 2.0 35.0 94-95 26.320258751402086 32.5 25.0 34.0 2.0 35.0 96-97 25.651840616882048 32.0 23.5 34.0 2.0 35.0 98-99 24.8343650323896 32.0 18.5 34.0 2.0 35.0 100-101 22.07690382500484 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2677382495170235 1101 2 0.2373407797873064 1101 3 0.09715671617547272 1101 4 0.03897828495046696 1101 5 0.07824951398343671 1101 6 0.13604717694144597 1101 7 0.13001203504195047 1101 8 0.07958857653061102 1101 9 0.251725194935986 1101 10-11 0.09417951275815994 1101 12-13 0.037714566336688904 1101 14-15 0.18326788644570513 1101 16-17 0.25546971903708027 1101 18-19 0.22418819713086435 1101 20-21 0.1345440365638666 1101 22-23 0.08201217158836727 1101 24-25 -0.018628325650105637 1101 26-27 0.24805832200105726 1101 28-29 0.02449606792227854 1101 30-31 -0.030306866511416786 1101 32-33 0.11087578272273646 1101 34-35 0.07584108307039372 1101 36-37 0.1634225196062431 1101 38-39 0.18871586813282448 1101 40-41 0.06616110029134603 1101 42-43 0.20481402739448384 1101 44-45 0.20636912955662723 1101 46-47 0.08859602514767317 1101 48-49 0.15586984834684614 1101 50-51 -0.051759238411143826 1101 52-53 0.038540632940787134 1101 54-55 0.07920350698031342 1101 56-57 -0.002192735857512673 1101 58-59 0.06580902889637485 1101 60-61 0.1143724574367937 1101 62-63 0.13044411188537453 1101 64-65 0.02247676240786234 1101 66-67 -0.13640555173225266 1101 68-69 -0.16313366532936158 1101 70-71 -0.22842898234320685 1101 72-73 0.017678727378861225 1101 74-75 -0.01772570119889494 1101 76-77 0.37085124292237026 1101 78-79 0.1316334657316709 1101 80-81 0.022580811590007244 1101 82-83 0.09743309033299852 1101 84-85 0.19079273516276274 1101 86-87 0.17273972780916935 1101 88-89 0.04273576286587044 1101 90-91 -0.047113096693113476 1101 92-93 -0.14211161073841438 1101 94-95 -0.21436565386875728 1101 96-97 -0.3921583016050434 1101 98-99 -0.2949639157923727 1101 100-101 -0.29229083969851644 1102 1 0.2986392891781726 1102 2 0.4114840219898461 1102 3 0.17531099675563055 1102 4 0.031245002958421253 1102 5 -0.019273268714549374 1102 6 0.0782497183353641 1102 7 0.06881963355304777 1102 8 -0.08356121168656472 1102 9 -0.0053085619630053316 1102 10-11 0.012822819137312536 1102 12-13 -0.03098329016440715 1102 14-15 0.15374343515575362 1102 16-17 0.20877481466023795 1102 18-19 0.1254974000573199 1102 20-21 0.11855122434464249 1102 22-23 -0.11323555014183029 1102 24-25 -0.09304752729302379 1102 26-27 -0.4433131322410162 1102 28-29 -0.3027982460297167 1102 30-31 -0.0905109481440789 1102 32-33 0.08485537455947423 1102 34-35 0.2157172222052921 1102 36-37 0.17573159417946727 1102 38-39 0.0626024038792039 1102 40-41 0.08974279712102629 1102 42-43 0.07303376812095763 1102 44-45 -0.16598679394484606 1102 46-47 -0.15222030138293974 1102 48-49 -0.0032432308470973226 1102 50-51 -0.05881866595574792 1102 52-53 -0.054162486042663716 1102 54-55 0.1834776694755078 1102 56-57 0.11681316839097633 1102 58-59 0.1753456737429886 1102 60-61 0.18509829494160357 1102 62-63 0.04560262812206162 1102 64-65 0.06723995070205291 1102 66-67 0.08217036920215648 1102 68-69 0.06754275618263961 1102 70-71 -0.0885335902240918 1102 72-73 -0.10102233795293003 1102 74-75 -0.483285321273339 1102 76-77 -0.16150018821612377 1102 78-79 0.06970303318129112 1102 80-81 -0.05596494633580562 1102 82-83 0.12504507852450075 1102 84-85 0.16269300470453985 1102 86-87 -0.1601828751942982 1102 88-89 -0.09217952400359408 1102 90-91 -0.088988337355417 1102 92-93 -0.016450591613782706 1102 94-95 -0.33228055567853687 1102 96-97 -0.2365268037228745 1102 98-99 -0.6281721047283213 1102 100-101 -0.8729062934271781 1103 1 0.44689282136027586 1103 2 0.4220805683006823 1103 3 0.22818252998430566 1103 4 0.0899838831258748 1103 5 0.028306532436680243 1103 6 -0.04934054854061998 1103 7 0.08474715840914371 1103 8 0.13339326241077032 1103 9 -0.0262138471540041 1103 10-11 0.01231915613364265 1103 12-13 0.10469829016003729 1103 14-15 0.09102757958275731 1103 16-17 0.0014978654190045404 1103 18-19 -0.056478659597310354 1103 20-21 -0.1944524386590274 1103 22-23 -0.10104298080640461 1103 24-25 -0.10262039490159935 1103 26-27 -0.006840081482252458 1103 28-29 -0.00829602206320601 1103 30-31 -0.02309532804988379 1103 32-33 -0.19853355793659944 1103 34-35 -0.09570022939072942 1103 36-37 0.029623535571417392 1103 38-39 0.03468181256575065 1103 40-41 -0.004697286604773865 1103 42-43 0.0994335588064672 1103 44-45 0.059922415892934566 1103 46-47 -0.07383529353365503 1103 48-49 -0.0026414516738881844 1103 50-51 -0.3534451704036812 1103 52-53 -0.24686264302852834 1103 54-55 -0.23737201655275442 1103 56-57 -0.1025516929381709 1103 58-59 -0.15291505362488067 1103 60-61 -0.19679927177738676 1103 62-63 -0.3653896534059342 1103 64-65 -0.3850755908992056 1103 66-67 -0.45191845863666913 1103 68-69 -0.5160260559575995 1103 70-71 -0.49093416583895433 1103 72-73 -0.4033247973982448 1103 74-75 -0.4244875714041605 1103 76-77 -0.49667156445892857 1103 78-79 -0.4381299076873617 1103 80-81 -0.30016955125469735 1103 82-83 -0.14214160384075214 1103 84-85 -0.2059196326580981 1103 86-87 -0.19765344034343357 1103 88-89 -0.22968933562054872 1103 90-91 -0.44657271726122616 1103 92-93 -0.18885378365983385 1103 94-95 -0.3020509638418005 1103 96-97 -0.255787007804507 1103 98-99 -0.40666176982513136 1103 100-101 -0.34140446192534313 1104 1 0.3676369310309191 1104 2 0.45566289182594133 1104 3 0.26633936245283607 1104 4 0.16603301231337753 1104 5 0.15561877084022058 1104 6 0.22770676220854824 1104 7 0.19827512473668207 1104 8 0.16059145651338724 1104 9 0.09476324992866125 1104 10-11 0.07559708718974889 1104 12-13 0.05479019183895417 1104 14-15 0.16544246569521448 1104 16-17 0.20805766617458943 1104 18-19 0.2147532735907589 1104 20-21 -0.007231670839118465 1104 22-23 0.11731070545027222 1104 24-25 0.20719750652649083 1104 26-27 0.4363650728498669 1104 28-29 0.28789240969049246 1104 30-31 0.2327357257037832 1104 32-33 0.14473048009384826 1104 34-35 0.02635411604985194 1104 36-37 0.16723013368086015 1104 38-39 0.05911952713363888 1104 40-41 0.11851170839452152 1104 42-43 0.19871088632915956 1104 44-45 0.11457256246533376 1104 46-47 0.057059444752319166 1104 48-49 0.3134258618969952 1104 50-51 0.33152263461583686 1104 52-53 0.13119225499783482 1104 54-55 0.19206307225287844 1104 56-57 0.28871676577857386 1104 58-59 0.35501563017063376 1104 60-61 0.09319314486446473 1104 62-63 -0.10757262200652207 1104 64-65 -0.06557323163222151 1104 66-67 0.2025562125551872 1104 68-69 0.09215142989463487 1104 70-71 0.19117518344229367 1104 72-73 0.24504644619410954 1104 74-75 0.321608076884786 1104 76-77 0.2675477219607849 1104 78-79 0.33470589595264855 1104 80-81 0.386096928342738 1104 82-83 0.5214879638098573 1104 84-85 0.5448205775596442 1104 86-87 0.4224090488791852 1104 88-89 0.478251929604955 1104 90-91 0.3357256281166485 1104 92-93 0.3757291904274247 1104 94-95 0.38607951302797616 1104 96-97 0.29582930562528986 1104 98-99 0.6072719159612632 1104 100-101 0.5949933057848327 1105 1 0.330261545067426 1105 2 0.3698507748045081 1105 3 0.23905457278866393 1105 4 0.06473874980213878 1105 5 -0.053661172347617025 1105 6 -0.03456256815128711 1105 7 0.05126326602787401 1105 8 0.01911234681981 1105 9 0.2046073694750774 1105 10-11 0.07289767877549025 1105 12-13 0.09882688718164445 1105 14-15 0.11282165159516921 1105 16-17 -0.014366319522693516 1105 18-19 0.017216795238965688 1105 20-21 0.02734723183060339 1105 22-23 0.11291482267105124 1105 24-25 -0.004206663436569613 1105 26-27 -0.007491904186480269 1105 28-29 -0.042435236196958215 1105 30-31 0.039770072561147174 1105 32-33 0.1465120896472527 1105 34-35 0.01933098211974027 1105 36-37 0.037168483345617176 1105 38-39 0.19347012214554837 1105 40-41 0.15475334282402287 1105 42-43 0.17498100989977416 1105 44-45 -0.11890394333735088 1105 46-47 0.029852003521547488 1105 48-49 0.4012126999968544 1105 50-51 0.21396599375966474 1105 52-53 0.3248218587869687 1105 54-55 0.2460849831433265 1105 56-57 0.404090000521208 1105 58-59 0.532697959635513 1105 60-61 0.2855810207830345 1105 62-63 0.3173921299489919 1105 64-65 0.47164538099644204 1105 66-67 0.25721715647601684 1105 68-69 0.019980150354285797 1105 70-71 0.1831994698121484 1105 72-73 0.20857276646438194 1105 74-75 0.24137231470681897 1105 76-77 0.23479247732170805 1105 78-79 0.10698432124236845 1105 80-81 0.02347241812963219 1105 82-83 0.3836078922962969 1105 84-85 0.3874694655072055 1105 86-87 0.2751734507421908 1105 88-89 0.32398956371882903 1105 90-91 0.21826294743797447 1105 92-93 0.3793266712564325 1105 94-95 0.4374933800966474 1105 96-97 0.47910460167771163 1105 98-99 0.39120970913259256 1105 100-101 0.4889776741336469 1106 1 0.30715950334748854 1106 2 0.23567894710818393 1106 3 0.0467421547701683 1106 4 0.028648070649381907 1106 5 -0.18989741190794973 1106 6 -0.06523596491131656 1106 7 2.4406644407548583E-4 1106 8 0.09309743656361036 1106 9 0.08703773016601701 1106 10-11 0.024302087650795556 1106 12-13 -0.09289969170600898 1106 14-15 0.01139067656182391 1106 16-17 0.06392082743797545 1106 18-19 -0.02183324322660951 1106 20-21 0.03168720116882895 1106 22-23 0.04402241007647234 1106 24-25 -0.12490004016128609 1106 26-27 -0.04604327372726402 1106 28-29 -0.28442443262603234 1106 30-31 -0.22148014190522503 1106 32-33 -0.24918668332831828 1106 34-35 -0.6772317444536142 1106 36-37 -0.3546413915167079 1106 38-39 -0.1912878214124376 1106 40-41 -0.21670685498316544 1106 42-43 -0.5291984555669913 1106 44-45 -0.36233712114796646 1106 46-47 -0.2829955011967087 1106 48-49 -0.4383432023946554 1106 50-51 -0.3259729040768562 1106 52-53 -0.21967424207587527 1106 54-55 -0.22202787875139052 1106 56-57 -0.5429511936275944 1106 58-59 -0.6417581259517888 1106 60-61 -0.3448660101720762 1106 62-63 -0.7595977029609458 1106 64-65 -0.35413818230020055 1106 66-67 -0.22101630470695 1106 68-69 -0.35232739720190764 1106 70-71 -0.16622299298139254 1106 72-73 0.010079289009642167 1106 74-75 0.08724570482531391 1106 76-77 0.24827840002525647 1106 78-79 0.23131421757900483 1106 80-81 0.07941825637493594 1106 82-83 0.16517595977433785 1106 84-85 0.19876035940307446 1106 86-87 0.10813015343920895 1106 88-89 0.15932974890709062 1106 90-91 0.1958762548693045 1106 92-93 0.18588768209874118 1106 94-95 0.272375299058794 1106 96-97 0.1904616342378631 1106 98-99 -0.07002694552395639 1106 100-101 -0.12636455886262965 1107 1 0.3923598543273066 1107 2 0.4314212276413407 1107 3 0.29755066185243706 1107 4 0.19383003697203094 1107 5 0.08297686210701016 1107 6 0.039945165745095323 1107 7 0.06620320236518751 1107 8 0.12020644922395718 1107 9 0.23037955943940602 1107 10-11 0.2250938814083696 1107 12-13 0.23725323521497899 1107 14-15 0.31850010705528575 1107 16-17 0.3210720412431769 1107 18-19 0.24221639534773942 1107 20-21 0.07917393496734348 1107 22-23 0.26598998622656467 1107 24-25 0.1118713633401498 1107 26-27 0.0788742042320365 1107 28-29 0.11677265925547431 1107 30-31 0.03452829832374249 1107 32-33 0.22763402448098446 1107 34-35 0.13500032005982376 1107 36-37 0.18031034875822627 1107 38-39 0.41414609828003535 1107 40-41 0.5049180980106129 1107 42-43 0.32965333902624394 1107 44-45 0.21308175655227757 1107 46-47 0.395738882290523 1107 48-49 0.22592997689753958 1107 50-51 0.03343669772818458 1107 52-53 -0.09466484083072402 1107 54-55 0.16613073069999018 1107 56-57 0.24174775761127876 1107 58-59 0.15182395736412957 1107 60-61 0.20409358536547018 1107 62-63 0.23605265428636812 1107 64-65 0.16849583767222498 1107 66-67 0.25048538751717686 1107 68-69 0.05045746052591582 1107 70-71 0.22815924075445082 1107 72-73 0.08019168611823702 1107 74-75 0.481968472551884 1107 76-77 0.29467458938722757 1107 78-79 0.5161970153895581 1107 80-81 0.512193086107942 1107 82-83 0.39982268187353043 1107 84-85 0.32533036734190546 1107 86-87 0.5175251310851365 1107 88-89 0.8632364885552768 1107 90-91 0.7646223376838286 1107 92-93 0.9766681943621442 1107 94-95 0.8145012339603994 1107 96-97 0.7088421130746134 1107 98-99 1.2484893290759658 1107 100-101 1.386617516096635 1108 1 0.2977353843091386 1108 2 0.24743533164756926 1108 3 0.1027120359114484 1108 4 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-0.48775378482159937 2316 62-63 -0.6007856982008377 2316 64-65 -0.5084240935416027 2316 66-67 -0.40298957263717483 2316 68-69 -0.7513198652017934 2316 70-71 -0.40006511122944133 2316 72-73 -0.537111811910453 2316 74-75 -0.6432349499797851 2316 76-77 -0.49724303497314537 2316 78-79 -0.3756220434142321 2316 80-81 -0.4996075178682382 2316 82-83 -0.3421746337879057 2316 84-85 -0.5455539963859728 2316 86-87 -0.3374671094987072 2316 88-89 -0.45977961747609797 2316 90-91 -0.5731953789506825 2316 92-93 -0.755957004866108 2316 94-95 -1.0633309529366777 2316 96-97 -1.1848696141294788 2316 98-99 -1.3518608932208203 2316 100-101 -2.325827958276321 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 24.0 5 50.0 6 85.0 7 233.0 8 537.0 9 1040.0 10 1773.0 11 2795.0 12 4047.0 13 5709.0 14 7159.0 15 9793.0 16 12690.0 17 16312.0 18 13508.0 19 8384.0 20 7406.0 21 7451.0 22 7636.0 23 8329.0 24 9938.0 25 11706.0 26 13980.0 27 17333.0 28 21578.0 29 26675.0 30 32503.0 31 40262.0 32 50161.0 33 64316.0 34 84183.0 35 114375.0 36 159757.0 37 202004.0 38 123686.0 39 7367.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.24032917172124 20.590140185318468 15.12445569975784 21.04507494320245 2 15.618999420838486 22.032436698742835 39.27893478129801 23.06962909912066 3 18.979209675356707 26.999835577397373 30.303725085408406 23.717229661837514 4 12.674377284341281 17.848944121620818 37.51558673257323 31.961091861464663 5 12.503607978107336 39.48379424111889 33.595391287509266 14.417206493264498 6 30.257874526395785 37.872078451145235 17.064274988216983 14.805772034241995 7 27.99087683915542 34.746016609487064 20.4675574262237 16.795549125133817 8 27.68579717134516 33.28647528854691 19.479793495774555 19.547934044333374 9 27.973422412462305 16.25484011520365 20.24714160311375 35.52459586922029 10-11 24.798785887541143 27.431752986755924 26.480932670741414 21.288528454961515 12-13 26.493681465479842 25.232440776583744 27.20577825072274 21.06809950721368 14-15 23.34457743725772 26.31266464254528 26.48383808490349 23.858919835293516 16-17 21.226648617642006 29.3795830456085 27.340573038264665 22.05319529848483 18-19 21.143756462391167 27.065133595665316 31.187682454082772 20.603427487860753 20-21 22.21920796203128 25.980730656670282 31.20188601346735 20.598175367831082 22-23 21.82503997372992 25.93544921977582 31.337760091853944 20.90175071464032 24-25 21.67070394849817 26.334860558496743 31.332869319946365 20.661566173058727 26-27 21.13029328308346 26.43557632452819 31.40723541519855 21.0268949771898 28-29 20.8322829093274 26.572073701401905 31.635482774873125 20.96016061439757 30-31 21.178420454586973 26.423415869257834 31.051812392445715 21.346351283709478 32-33 20.998872806128137 26.528334219377136 31.05341473974975 21.419378234744972 34-35 21.55447305371172 26.379649411895805 31.10174654659482 20.96413098779765 36-37 22.24698636155623 25.87079695973503 30.729658111772945 21.152558566935795 38-39 21.485565112388567 26.47494440742586 30.85941621060737 21.1800742695782 40-41 21.382351168020694 26.591538951987566 31.09801435568409 20.928095524307654 42-43 21.511161861261776 26.68117613474165 30.086029117898942 21.72163288609763 44-45 21.445743844191384 26.62277044042679 30.235280362520655 21.69620535286117 46-47 22.315209110888233 27.26132604053059 29.08763237843654 21.335832470144634 48-49 22.16893961470265 27.519875544333676 28.433332998382188 21.877851842581485 50-51 22.12968308135244 27.6153369772523 28.603789578294325 21.651190363100937 52-53 22.424709380790294 27.571385705458063 28.41110201233222 21.592802901419425 54-55 21.798942717273246 27.84447329068515 28.483876301392264 21.872707690649342 56-57 22.367855559011414 28.02727724881789 27.68281771733218 21.922049474838516 58-59 22.22935198662385 27.30468873097856 27.988887310699045 22.47707197169854 60-61 21.84318423575483 27.78953358840372 28.520489746106463 21.84679242973499 62-63 21.82424541645905 27.834743612638206 28.857205623700917 21.48380534720183 64-65 21.66432795978886 27.8289674426423 28.490565692997215 22.016138904571626 66-67 21.729328905620946 27.85731999782603 28.139205304485916 22.274145792067113 68-69 22.124918363711927 27.627135673801945 28.17755835567389 22.070387606812233 70-71 22.131412957150054 27.858014195474823 27.996443138861448 22.01412970851368 72-73 21.917096883803012 27.736263194238926 28.011291601357698 22.335348320600367 74-75 21.92335375722966 28.556050624956615 27.457900832666542 22.062694785147187 76-77 21.953234610662925 27.961360414474452 28.014795612650534 22.07060936221209 78-79 21.950859679276576 27.86769485005456 27.81617301470793 22.365272455960934 80-81 22.28456139421307 28.41128445085549 27.181746676301504 22.122407478629942 82-83 22.37748822601663 28.38385264010823 27.20930901756266 22.029350116312475 84-85 21.816575752468566 28.477587971647377 27.26635781873522 22.439478457148834 86-87 21.92152415265095 28.5949446123504 26.978921497204468 22.504609737794187 88-89 21.737402438996533 28.41570295315473 26.960253812787943 22.886640795060792 90-91 21.61163476762533 28.274482502807096 27.33811339302926 22.775769336538314 92-93 21.98400396064565 28.218093098922058 27.333798575227146 22.46410436520514 94-95 21.52862052822088 27.832421754570262 27.580392112084223 23.058565605124635 96-97 22.067506443572665 27.500980866368774 27.493809922485298 22.937702767573263 98-99 21.407286961208374 27.994738260428704 27.06936635318513 23.528608425177787 100-101 21.027623103158316 28.168193214190612 27.30497409520486 23.499209587446213 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 483.0 1 356.5 2 257.0 3 310.5 4 313.0 5 311.5 6 487.0 7 897.5 8 1332.0 9 1601.5 10 1518.5 11 1371.5 12 1435.5 13 1527.5 14 1681.0 15 1828.5 16 1997.0 17 2138.5 18 2267.5 19 2475.5 20 2725.0 21 3038.0 22 3466.5 23 3986.0 24 4480.5 25 5338.0 26 6718.0 27 8288.0 28 9488.5 29 11519.0 30 14440.0 31 17236.0 32 21810.0 33 25019.5 34 27129.0 35 30258.5 36 34991.5 37 38768.0 38 39061.0 39 38303.0 40 36186.0 41 33988.5 42 31487.0 43 31974.0 44 33457.0 45 34179.0 46 34016.5 47 33994.5 48 38674.0 49 37583.0 50 32177.0 51 29952.0 52 29227.5 53 30977.0 54 32427.5 55 30790.5 56 27322.5 57 24939.0 58 24748.5 59 23131.0 60 19265.0 61 16258.5 62 13987.0 63 10388.5 64 6871.0 65 5313.5 66 4277.5 67 3533.0 68 2830.5 69 2271.0 70 1862.0 71 1355.5 72 1093.5 73 1033.5 74 803.0 75 568.5 76 432.5 77 301.5 78 206.5 79 147.5 80 112.0 81 88.5 82 62.5 83 37.5 84 20.5 85 12.5 86 5.0 87 3.0 88 3.5 89 2.5 90 2.0 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.1365587744200747 2 0.01004753396979894 3 0.0051151082028067325 4 0.010504240059335255 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0035623074983832607 10-11 4.1103548058268386E-4 12-13 9.13412179072631E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0013244476596553148 24-25 0.0 26-27 4.567060895363155E-5 28-29 0.0 30-31 0.0 32-33 0.0038363311521050496 34-35 6.393885253508416E-4 36-37 0.002100848011867051 38-39 4.567060895363155E-5 40-41 0.0034709662804759973 42-43 0.009910522142938045 44-45 0.0015528007044234725 46-47 0.0016441419223307354 48-49 0.009956192751891677 50-51 3.653648716290524E-4 52-53 0.0019638361850061563 54-55 0.009408145444448098 56-57 0.0034252956715223656 58-59 0.0010504240059335255 60-61 0.005982849772925732 62-63 0.0033339544536151026 64-65 0.015893371915863777 66-67 0.0022378598387279456 68-69 9.13412179072631E-5 70-71 9.590827880262624E-4 72-73 0.0 74-75 0.0 76-77 0.0010960946148871572 78-79 0.010458569450381624 80-81 0.014340571211440304 82-83 0.010549910668288887 84-85 0.007535650477349205 86-87 0.009362474835494466 88-89 0.0263519413662454 90-91 0.022743963258908508 92-93 0.0022835304476815773 94-95 0.007626991695256468 96-97 0.009179792399679941 98-99 0.009225463008633571 100-101 0.010458569450381624 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1094796.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.957018192225206 #Duplication Level Percentage of deduplicated Percentage of total 1 74.13960835311686 31.84816504789293 2 13.090904816830836 11.246924727385817 3 4.83420773577853 6.229894489525024 4 2.299486440662799 3.9511632345730825 5 1.2988587209686373 2.7897548852890055 6 0.8445984672203786 2.1768859032906795 7 0.5627534458947924 1.692194700912801 8 0.3941560703700423 1.354541558836952 9 0.3155840337974273 1.2200894172910708 >10 1.8569657660145087 15.399891086430006 >50 0.222013866575359 6.6298537594619456 >100 0.12973030918153314 10.265589155666188 >500 0.008348980191220792 2.3656007666275904 >1k 0.002354840566754582 1.68361914303444 >5k 4.281528303190149E-4 1.1458321237824514 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6829 0.6237691770886997 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5631 0.5143423980357984 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3572 0.32627083036474375 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2363 0.21583929791486267 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2060 0.18816290888896195 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1580 0.14431912429347568 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1471 0.134362931541584 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1437 0.13125733013273708 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1428 0.1304352591715717 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 1188 0.10851336687382855 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1139 0.10403764719637265 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 9.13412179072631E-5 0.0 8 0.0 0.0 0.0 9.13412179072631E-5 0.0 9 0.0 0.0 0.0 9.13412179072631E-5 0.0 10-11 0.0 0.0 0.0 9.13412179072631E-5 0.0 12-13 0.0 0.0 0.0 9.13412179072631E-5 0.0 14-15 0.0 0.0 0.0 9.13412179072631E-5 0.0 16-17 0.0 0.0 0.0 9.13412179072631E-5 0.0 18-19 0.0 0.0 0.0 9.13412179072631E-5 0.0 20-21 0.0 0.0 0.0 1.3701182686089465E-4 0.0 22-23 0.0 0.0 0.0 3.1969426267542084E-4 0.0 24-25 0.0 0.0 0.0 5.02376698489947E-4 0.0 26-27 0.0 0.0 0.0 8.677415701189993E-4 0.0 28-29 0.0 0.0 0.0 0.0016898125312843671 0.0 30-31 0.0 0.0 0.0 0.003014260190939682 0.0 32-33 0.0 0.0 0.0 0.005800167337111206 0.0 34-35 0.0 0.0 0.0 0.007535650477349205 0.0 36-37 0.0 0.0 0.0 0.010001863360845308 0.0 38-39 0.0 0.0 0.0 0.014020876948764884 0.0 40-41 0.0 0.0 0.0 0.01986671489482972 0.0 42-43 0.0 0.0 0.0 0.03164973200486666 0.0 44-45 0.0 0.0 0.0 0.04813682183712765 0.0 46-47 0.0 0.0 0.0 0.06434988801566685 0.0 48-49 0.0 0.0 0.0 0.09536023149518266 0.0 50-51 0.0 0.0 0.0 0.1284257523776119 0.0 52-53 0.0 0.0 0.0 0.1627243797017892 0.0 54-55 0.0 0.0 0.0 0.22410567813547 0.0 56-57 0.0 0.0 0.0 0.28366015221100555 0.0 58-59 0.0 0.0 0.0 0.3639947533604434 0.0 60-61 0.0 0.0 0.0 0.45620371283782546 0.0 62-63 0.0 0.0 0.0 0.5784182623977435 0.0 64-65 0.0 0.0 0.0 0.6753769652063033 0.0 66-67 0.0 0.0 0.0 0.8153573816491839 0.0 68-69 0.0 0.0 0.0 0.9878552716670503 0.0 70-71 0.0 0.0 0.0 1.100296310910891 0.0 72-73 0.0 0.0 0.0 1.266400315675249 0.0 74-75 0.0 0.0 0.0 1.4779009057395167 0.0 76-77 0.0 0.0 0.0 1.6268784321462628 0.0 78-79 0.0 0.0 0.0 1.7705581679143876 0.0 80-81 0.0 0.0 0.0 1.9940701281334605 0.0 82-83 0.0 0.0 0.0 2.273071878231195 0.0 84-85 0.0 0.0 0.0 2.6048688522793286 0.0 86-87 0.0 0.0 0.0 3.07372332379731 0.0 88-89 0.0 0.0 0.0 3.5867412741734532 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTAAG 580 0.0 31.939964 2 TAAGGTA 325 0.0 29.22571 4 GGTATCA 8085 0.0 27.102617 1 GGTAAGG 720 0.0 27.045164 3 GTATCAA 11155 0.0 25.8038 1 GTACATG 8190 0.0 23.938818 1 GTAAGGT 855 0.0 23.884754 4 TAAGGTG 825 0.0 23.600895 5 TACATGG 8305 0.0 22.649225 2 ACATGGG 8120 0.0 22.34321 3 GAGTACT 6990 0.0 22.1822 12-13 GGTGTGC 1085 0.0 21.884611 8 GTATAGG 550 0.0 21.842571 1 CATATAG 285 8.367351E-11 21.663906 3 ATACCGT 110 0.002880935 21.586185 6 GTACTTT 7185 0.0 21.481033 14-15 ATAGGGG 400 0.0 21.372276 3 ATCAACG 13590 0.0 21.213264 3 TATAGGG 405 0.0 21.111315 2 TCAACGC 13680 0.0 21.07274 4 >>END_MODULE