FastQCFastQC Report
Thu 26 May 2016
SRR936653_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936653_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143516
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70580.617219173146681No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52500.4591103228988488No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32310.28254961014974866No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17060.14918899254579734No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13290.11622049888239432No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA12830.11219781795794724No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12800.11193546920200503No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG11650.1018787668908874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98750.030.3713361
TAAGGTG5250.028.9709245
ATACCGT1153.8466387E-628.9302926
GGTATCA66900.028.063551
GTACATG78900.028.0122281
TACATGG78450.027.6906382
AGGTAAG4300.027.6363872
ACATGGG77550.026.2957273
ATCAACG118150.025.3866443
TCAACGC118650.025.1995374
CAACGCA118600.025.167885
AACGCAG121150.024.7939836
TATCAAC121550.024.7156372
GATCGTG1751.4769648E-724.4442187
ATGGGCG2151.6498234E-924.3178925
CATGGGG24650.024.1047574
TAACACG1000.001646649223.7672924
GAGTACT59800.023.6072812-13
GGTAAGG4850.023.5222663
CATGGGA47400.023.1655884
Produced by FastQC (version 0.11.5)