##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936653_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1143516 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50594132482624 31.0 31.0 34.0 30.0 34.0 2 31.54215070012138 31.0 31.0 34.0 30.0 34.0 3 31.563416690278054 33.0 31.0 34.0 28.0 34.0 4 35.233847187096636 37.0 35.0 37.0 33.0 37.0 5 35.20868181993081 37.0 35.0 37.0 33.0 37.0 6 35.35255912466463 37.0 35.0 37.0 33.0 37.0 7 35.218434197685035 37.0 35.0 37.0 32.0 37.0 8 35.16665879620399 37.0 35.0 37.0 32.0 37.0 9 36.658323976227706 39.0 37.0 39.0 32.0 39.0 10-11 36.57213628842972 39.0 37.0 39.0 32.0 39.0 12-13 36.48417293680193 39.0 37.0 39.0 32.0 39.0 14-15 37.51994200343502 40.0 37.0 41.0 32.0 41.0 16-17 37.41610392858517 40.0 37.0 41.0 32.0 41.0 18-19 37.44189718377355 40.0 37.0 41.0 32.0 41.0 20-21 37.365669566494915 40.0 37.0 41.0 31.5 41.0 22-23 37.24105827990164 39.0 36.5 41.0 31.0 41.0 24-25 37.34125845200242 40.0 37.0 41.0 32.0 41.0 26-27 37.305318421430044 40.0 37.0 41.0 31.5 41.0 28-29 37.21233371461352 39.5 36.5 41.0 31.0 41.0 30-31 36.98077508316456 39.0 36.0 41.0 31.0 41.0 32-33 36.75502703941178 39.0 35.5 41.0 30.5 41.0 34-35 36.51825684992602 39.0 35.0 41.0 30.0 41.0 36-37 36.304742128662824 39.0 35.0 40.0 30.0 41.0 38-39 36.06944939992094 38.0 35.0 40.0 30.0 41.0 40-41 35.85776849646179 38.0 35.0 40.0 29.0 41.0 42-43 35.57401558001812 38.0 35.0 40.0 27.5 41.0 44-45 35.22195885322112 38.0 34.5 40.0 26.5 41.0 46-47 34.808861441379044 38.0 34.0 40.0 24.5 41.0 48-49 34.59717397920099 38.0 34.0 40.0 24.0 41.0 50-51 33.64103694220282 37.5 33.0 39.5 22.5 40.5 52-53 33.76535658442907 37.5 33.0 39.5 23.0 40.5 54-55 34.17491403705763 38.0 34.0 40.0 23.0 41.0 56-57 34.07118265070187 38.0 34.0 40.0 22.0 41.0 58-59 33.817588035497536 38.0 33.5 40.0 21.5 41.0 60-61 33.49715613948559 37.0 33.0 40.0 20.0 41.0 62-63 33.169255174391964 37.0 33.0 40.0 18.5 41.0 64-65 32.822088191157796 36.0 32.5 39.0 18.0 41.0 66-67 32.431345954057484 36.0 32.0 39.0 14.0 41.0 68-69 32.021484614119956 35.0 31.5 39.0 11.5 40.0 70-71 31.57883317767307 35.0 31.0 38.0 8.0 40.0 72-73 31.209344250539566 35.0 31.0 37.0 7.0 40.0 74-75 30.798527086634557 35.0 30.5 37.0 4.5 39.0 76-77 30.372484075430513 35.0 30.0 36.0 2.0 39.0 78-79 29.94156356360558 34.0 30.0 36.0 2.0 38.5 80-81 29.49252043696809 34.0 29.0 35.5 2.0 37.0 82-83 29.036885360589622 34.0 29.0 35.0 2.0 37.0 84-85 28.70632461635867 34.0 29.0 35.0 2.0 36.5 86-87 28.407787035773875 34.0 29.0 35.0 2.0 36.0 88-89 28.00350716561902 34.0 27.5 35.0 2.0 36.0 90-91 27.606907555294374 34.0 27.0 35.0 2.0 35.0 92-93 27.25776945840723 33.5 26.0 35.0 2.0 35.0 94-95 26.82428317574918 33.0 25.0 35.0 2.0 35.0 96-97 26.269371832138773 33.0 24.5 35.0 2.0 35.0 98-99 25.59570176543223 33.0 22.0 35.0 2.0 35.0 100-101 23.188633128001708 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34704981335922724 1101 2 0.41639459413364577 1101 3 0.3404057214799039 1101 4 0.3233381236308688 1101 5 0.29974622009309826 1101 6 0.14684404686421715 1101 7 0.18316402283149102 1101 8 0.2825224705633076 1101 9 0.5833611722693277 1101 10-11 0.48578471913497623 1101 12-13 0.5738192234642625 1101 14-15 0.5160601879267972 1101 16-17 0.591472907409198 1101 18-19 0.4475058738525419 1101 20-21 0.567889031412399 1101 22-23 0.43029820592971646 1101 24-25 0.4486983931021271 1101 26-27 0.3937753109723161 1101 28-29 0.38799900438619517 1101 30-31 0.5501972096292178 1101 32-33 0.4701846902087894 1101 34-35 0.44852588701592566 1101 36-37 0.41084297908572864 1101 38-39 0.31007963002304706 1101 40-41 0.46956287869922875 1101 42-43 0.5330470428180831 1101 44-45 0.5400346351545693 1101 46-47 0.7275480206237148 1101 48-49 0.8952031081010574 1101 50-51 0.895371986723319 1101 52-53 0.9108084335159745 1101 54-55 0.9790289991577907 1101 56-57 0.7628612941774122 1101 58-59 0.8799467901972022 1101 60-61 0.8594375463020256 1101 62-63 0.7463119897642088 1101 64-65 0.801820284202428 1101 66-67 0.7488394557209688 1101 68-69 0.8328017057542887 1101 70-71 0.719165828404968 1101 72-73 0.7605705751764198 1101 74-75 0.7602773471096249 1101 76-77 0.6541553416681474 1101 78-79 0.3895473679609154 1101 80-81 0.6431631425146342 1101 82-83 0.7425792462349676 1101 84-85 0.7637674320746335 1101 86-87 0.6372694011987328 1101 88-89 0.4881964545056654 1101 90-91 0.40695433495082156 1101 92-93 0.40141639687691466 1101 94-95 0.4663708115900498 1101 96-97 0.3603213725480572 1101 98-99 0.2876190759718007 1101 100-101 0.7961530223236046 1102 1 0.2467169516294696 1102 2 0.2909571738280299 1102 3 0.20960306862989597 1102 4 0.19366722805430925 1102 5 0.3504675726868527 1102 6 0.30558224935609246 1102 7 0.3562343173303475 1102 8 0.14830491476639907 1102 9 0.4150069135246923 1102 10-11 0.29842045353770175 1102 12-13 0.3736358322191009 1102 14-15 0.3898562406094399 1102 16-17 0.3616542761917714 1102 18-19 0.36909070839639213 1102 20-21 0.3665054022197225 1102 22-23 0.28262670863630035 1102 24-25 0.2171426354524968 1102 26-27 0.23693767545476874 1102 28-29 0.2934363242534914 1102 30-31 0.20826430151521436 1102 32-33 0.2467918289081794 1102 34-35 0.19039871475187198 1102 36-37 0.05783583289912286 1102 38-39 0.006148474101230761 1102 40-41 0.2204025658977642 1102 42-43 0.3615020411996568 1102 44-45 0.15561289281447443 1102 46-47 0.3873173137981496 1102 48-49 0.6013016317033788 1102 50-51 0.5730501175113645 1102 52-53 0.4620653049809462 1102 54-55 0.5620609033907655 1102 56-57 0.5944367286073913 1102 58-59 0.7413857335130061 1102 60-61 0.4856974195032038 1102 62-63 0.48862332208311443 1102 64-65 0.3983428812269878 1102 66-67 0.44892834363590595 1102 68-69 0.3601373646913082 1102 70-71 0.2557155620379703 1102 72-73 0.2438681387709991 1102 74-75 0.1661545411733556 1102 76-77 0.4088176200932203 1102 78-79 0.23294051231498258 1102 80-81 0.5408486783462365 1102 82-83 0.41622455012709025 1102 84-85 0.6275465882922902 1102 86-87 0.39326613969249635 1102 88-89 0.4086620865663093 1102 90-91 0.6710596417921799 1102 92-93 0.6605897255718247 1102 94-95 0.7817510231405542 1102 96-97 0.7117151140536038 1102 98-99 0.8496769476198445 1102 100-101 0.7459770041643559 1103 1 0.30211422277631783 1103 2 0.4116090835651782 1103 3 0.4184122426919892 1103 4 0.34230147706203695 1103 5 0.5870564737149238 1103 6 0.36763784412776346 1103 7 0.2498382279337079 1103 8 0.21995578216547074 1103 9 0.31723904123735736 1103 10-11 0.47946965068644687 1103 12-13 0.31960782060328086 1103 14-15 0.37174125998539864 1103 16-17 0.3971839238090098 1103 18-19 0.3031336649932044 1103 20-21 0.5611712150645118 1103 22-23 0.8588042880977369 1103 24-25 0.5599461577465874 1103 26-27 0.4423609934357273 1103 28-29 0.15132679557338946 1103 30-31 0.18043415382664563 1103 32-33 0.5069931957977047 1103 34-35 0.3619618454465652 1103 36-37 0.4176885300360098 1103 38-39 0.4601736045328977 1103 40-41 0.4434675603003697 1103 42-43 0.4877999100992767 1103 44-45 0.6076683621152341 1103 46-47 0.7309628853948809 1103 48-49 0.911022189370513 1103 50-51 0.8889459357514014 1103 52-53 0.898180390628788 1103 54-55 0.9806716335711272 1103 56-57 0.9876914118095783 1103 58-59 0.8198752308997257 1103 60-61 0.632402901606639 1103 62-63 0.9405858499597315 1103 64-65 0.9285583705659164 1103 66-67 0.8071248245947729 1103 68-69 1.014292798999616 1103 70-71 0.9719586559092761 1103 72-73 1.0634418815175053 1103 74-75 0.7696692140683368 1103 76-77 0.8299895835690982 1103 78-79 0.8913724139011663 1103 80-81 0.7490546625657117 1103 82-83 0.7066583681654421 1103 84-85 0.8561004708505777 1103 86-87 0.8573621704895693 1103 88-89 0.7970575597613063 1103 90-91 0.9879457509063378 1103 92-93 0.7931135081204914 1103 94-95 0.8386934454635941 1103 96-97 0.8370015479050963 1103 98-99 0.9804613314158814 1103 100-101 1.6688569986622213 1104 1 0.13045157979161104 1104 2 0.2261332173117765 1104 3 0.18717538020177216 1104 4 0.21644793777640814 1104 5 0.41240282289852104 1104 6 0.13470838439420163 1104 7 0.17695247726584995 1104 8 0.19256790936945833 1104 9 0.3100425575983152 1104 10-11 0.36558456075984935 1104 12-13 0.09550730742429181 1104 14-15 0.34021123651091756 1104 16-17 0.1907336949058518 1104 18-19 0.4388504612718478 1104 20-21 0.531400059317086 1104 22-23 0.44581694232388 1104 24-25 0.45419908322026714 1104 26-27 0.05507825017151191 1104 28-29 0.3750333675436295 1104 30-31 0.32070471412149715 1104 32-33 0.0595397986745283 1104 34-35 0.15449890537373534 1104 36-37 0.32039216941462456 1104 38-39 0.30433684395195826 1104 40-41 0.3504454004323989 1104 42-43 0.5642443857426684 1104 44-45 0.4595432768226573 1104 46-47 0.27244428106108387 1104 48-49 0.3150114761332006 1104 50-51 0.17929459703854178 1104 52-53 0.43214229540939897 1104 54-55 0.41600857725495644 1104 56-57 0.40859317146273355 1104 58-59 0.41778103452506343 1104 60-61 0.30637151197841206 1104 62-63 0.6339876660655221 1104 64-65 0.3374310556397404 1104 66-67 0.43677222635000845 1104 68-69 0.3993935252259426 1104 70-71 0.5374532840729529 1104 72-73 0.5179513670327367 1104 74-75 0.12974326028880867 1104 76-77 0.3974070375454737 1104 78-79 0.30840471364403754 1104 80-81 0.23165620612800097 1104 82-83 0.428210658522449 1104 84-85 0.5231170620343448 1104 86-87 0.8141238706661902 1104 88-89 0.8654648984074065 1104 90-91 0.8774333377083998 1104 92-93 0.8620623011631743 1104 94-95 0.6798111879865658 1104 96-97 0.7621087833655977 1104 98-99 1.0496042724312211 1104 100-101 1.4739071531460866 1105 1 0.1631713904058536 1105 2 0.18179116066929524 1105 3 0.2081742606854533 1105 4 0.3239461751686932 1105 5 0.3984927504314939 1105 6 0.24052296135189977 1105 7 0.2506333175280844 1105 8 0.26260072731592743 1105 9 0.4639195818559614 1105 10-11 0.3234453525791636 1105 12-13 0.24943230312057096 1105 14-15 1.6171902135653227E-4 1105 16-17 0.11734835870556992 1105 18-19 0.12723265602821243 1105 20-21 -0.05841782465453349 1105 22-23 -0.11510349526336228 1105 24-25 0.17900804724552444 1105 26-27 0.18286977671589 1105 28-29 0.06840815550987145 1105 30-31 -0.07637105662875143 1105 32-33 -0.023904737022249378 1105 34-35 -0.1821547825059895 1105 36-37 -0.07530695810826415 1105 38-39 -0.11124109949327732 1105 40-41 -0.1737333011260418 1105 42-43 0.2876013228512875 1105 44-45 0.11554995883287944 1105 46-47 0.2198379790943079 1105 48-49 0.35627897474340386 1105 50-51 0.27732683603827724 1105 52-53 0.21855864802304126 1105 54-55 0.2185737608457643 1105 56-57 0.4714497363129908 1105 58-59 0.201694922777385 1105 60-61 -0.0803684696663467 1105 62-63 0.0865776303309147 1105 64-65 0.2741131426111352 1105 66-67 0.4521321327536185 1105 68-69 0.2743191009654389 1105 70-71 0.4746740217695127 1105 72-73 0.515783058006761 1105 74-75 0.3719091131771286 1105 76-77 0.2150467660106834 1105 78-79 0.3132161118771535 1105 80-81 0.11354531589871542 1105 82-83 0.14994753161798258 1105 84-85 0.24695974422438738 1105 86-87 0.3300640944159454 1105 88-89 0.47501083092283025 1105 90-91 0.45810238302743045 1105 92-93 0.4499350118290586 1105 94-95 0.5478847975549748 1105 96-97 0.6909457583250571 1105 98-99 0.9026951681648896 1105 100-101 0.9027254877751041 1106 1 -0.02194758530175278 1106 2 -0.03265751119517191 1106 3 0.011033693036132775 1106 4 -0.040025350735398035 1106 5 0.03050994180989619 1106 6 -0.0911160967407838 1106 7 0.06950753203629034 1106 8 -0.010664916715853678 1106 9 0.1664630957816442 1106 10-11 0.2017335918110561 1106 12-13 0.17913417954644473 1106 14-15 0.40296354718110905 1106 16-17 0.3991971439107971 1106 18-19 0.33418898948352904 1106 20-21 0.10143613967522924 1106 22-23 0.0076106484623039705 1106 24-25 -0.1012938290636285 1106 26-27 -0.2881536227251047 1106 28-29 0.043909157309208524 1106 30-31 -0.12397622221308069 1106 32-33 -0.4258433777617725 1106 34-35 -0.4738782800550396 1106 36-37 -0.4968333582782165 1106 38-39 -0.46601346372218444 1106 40-41 -0.38121898616359573 1106 42-43 -0.6285247573731993 1106 44-45 -0.568077260927403 1106 46-47 -0.3791977489275311 1106 48-49 -0.28117121980935167 1106 50-51 -0.15584004627934434 1106 52-53 -0.181693888283057 1106 54-55 -0.1182301228556355 1106 56-57 -0.27899192499887704 1106 58-59 -0.33917020229141315 1106 60-61 -0.12101852741644592 1106 62-63 -0.011781963393204364 1106 64-65 -0.04438112936119154 1106 66-67 0.11644531512545342 1106 68-69 -0.14192341326198132 1106 70-71 0.039938344465038966 1106 72-73 0.22802765462276398 1106 74-75 0.08986422859853604 1106 76-77 0.10249127017983994 1106 78-79 0.29631654802317087 1106 80-81 0.18738898617862887 1106 82-83 0.1896830425881646 1106 84-85 0.16899798710396752 1106 86-87 0.13869328392439684 1106 88-89 0.36558621333119845 1106 90-91 0.37215045188632345 1106 92-93 0.3097750274236297 1106 94-95 0.27022384286618717 1106 96-97 0.3773717713738378 1106 98-99 0.8248063646803914 1106 100-101 0.9530742936991743 1107 1 0.10815532871300704 1107 2 0.19054931244012607 1107 3 0.043970347835454504 1107 4 0.20249761509256814 1107 5 0.30055541301985755 1107 6 0.29507643221952407 1107 7 0.33447072242287135 1107 8 0.31776336404845296 1107 9 0.19964126751582256 1107 10-11 0.23968563245540508 1107 12-13 0.28214462732579904 1107 14-15 0.12382209644622577 1107 16-17 0.277862684656796 1107 18-19 0.427795914590682 1107 20-21 0.5438074189678446 1107 22-23 0.45503119547210247 1107 24-25 0.4944363600319761 1107 26-27 0.39242459314237976 1107 28-29 0.5355776918226667 1107 30-31 0.5004805818749816 1107 32-33 0.3416305462281528 1107 34-35 0.4523799971125726 1107 36-37 0.4745355909841109 1107 38-39 0.42354379577299284 1107 40-41 0.4165800821717909 1107 42-43 0.8350183481870772 1107 44-45 0.7708526626733203 1107 46-47 0.7298827665634349 1107 48-49 0.7091003390521422 1107 50-51 0.7364321068180715 1107 52-53 0.686506497796529 1107 54-55 0.7387536260084602 1107 56-57 0.8389022210823782 1107 58-59 0.7777726450716216 1107 60-61 0.8348507684834985 1107 62-63 0.9206071951027184 1107 64-65 1.0289010162822123 1107 66-67 0.7886121390387544 1107 68-69 0.9421340364929414 1107 70-71 0.9671192510744362 1107 72-73 0.8435987205464599 1107 74-75 0.7745821142307108 1107 76-77 0.6747456657011774 1107 78-79 0.7587458956320638 1107 80-81 0.8798745081940247 1107 82-83 0.9358131246517978 1107 84-85 1.0466948538126815 1107 86-87 0.9683004800865369 1107 88-89 0.7764447452209886 1107 90-91 0.7549660875884356 1107 92-93 0.8734234274173716 1107 94-95 1.0315425600811317 1107 96-97 1.1350833723531082 1107 98-99 1.293151139661319 1107 100-101 1.7004988370323488 1108 1 -0.040270310388240915 1108 2 0.07500093499574945 1108 3 -0.04146675253464949 1108 4 0.09464445079639461 1108 5 0.25038691544430947 1108 6 0.1547016416151692 1108 7 -0.1960642512478472 1108 8 -0.04930750058692013 1108 9 0.09629302202441892 1108 10-11 -0.07939929326585826 1108 12-13 0.08319485530031301 1108 14-15 0.1255420225721693 1108 16-17 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-2.368675724986268 2316 56-57 -1.570836660470647 2316 58-59 -1.8529144759886194 2316 60-61 -1.1569591780299646 2316 62-63 -0.8873528732015004 2316 64-65 -1.2680103149817157 2316 66-67 -1.4164065155709764 2316 68-69 -2.336018984309426 2316 70-71 -2.274920565398311 2316 72-73 -1.7833136938140157 2316 74-75 -1.338214325838333 2316 76-77 -1.5463731039011428 2316 78-79 -2.1782824100592606 2316 80-81 -1.9416945530378023 2316 82-83 -1.7730228691814638 2316 84-85 -1.6410000171490395 2316 86-87 -2.162201131883755 2316 88-89 -2.6748781304694838 2316 90-91 -2.4601253649362143 2316 92-93 -2.5634124558017746 2316 94-95 -3.39563993707862 2316 96-97 -4.288353223866192 2316 98-99 -5.45666392736392 2316 100-101 -6.0341632483663545 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2156.0 3 793.0 4 825.0 5 932.0 6 1039.0 7 1185.0 8 1561.0 9 2023.0 10 2718.0 11 3532.0 12 4838.0 13 6195.0 14 7326.0 15 9511.0 16 12373.0 17 16045.0 18 12743.0 19 7950.0 20 7594.0 21 8109.0 22 8781.0 23 9900.0 24 11196.0 25 12856.0 26 14615.0 27 17800.0 28 21152.0 29 25239.0 30 30452.0 31 37914.0 32 47519.0 33 61254.0 34 82002.0 35 113047.0 36 157081.0 37 201282.0 38 157536.0 39 24440.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.55437230027975 19.315607247890522 14.595765823925316 21.534254627904414 2 15.39513838655871 20.631199799174833 40.42927254231892 23.54438927194754 3 18.531848977785398 26.729682970903575 30.263569905414318 24.47489814589671 4 12.053331606825074 17.014826389678745 38.474769939858014 32.45707206363816 5 12.572228541429698 38.69577390847843 34.20194242715898 14.53005512293289 6 30.00312338417363 37.252349318586056 17.973544848559612 14.770982448680698 7 27.62509197456821 34.24638597909498 21.206513855460162 16.922008190876646 8 27.008290983151202 32.95974188333133 20.460102760249125 19.571864373268337 9 27.627261139587233 16.098762710366795 20.90724658220938 35.36672956783659 10-11 24.48103791978589 26.867347023587936 27.32452972649967 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21.843863953693283 25.732594205363824 30.73736279641899 21.686179044523904 38-39 21.246558521747342 26.226556842550842 30.811856526651997 21.71502810904982 40-41 21.261892799846784 26.240263962329575 31.152084855528635 21.345758382295006 42-43 21.231281023391507 26.22016594781599 30.465801247407065 22.08275178138544 44-45 21.283783488340568 26.29845342568179 30.447400294710512 21.970362791267124 46-47 22.007692970293697 26.715428747327856 29.597209997450836 21.67966828492761 48-49 21.698675036270128 27.07133256318523 28.98221517564886 22.24777722489578 50-51 21.908627186573305 27.043024434352738 29.110993928359363 21.937354450714597 52-53 22.125757771862133 27.087086483064038 28.843910365164703 21.943245379909122 54-55 21.614091058757783 27.196896056096975 28.919973221356276 22.269039663788966 56-57 21.902456239474493 27.431717312274813 28.269778331898227 22.396048116352464 58-59 21.959573803782874 26.72839392802451 28.680743770645357 22.631288497547263 60-61 21.59329030452502 27.191145860663624 29.11410505326127 22.101458781550086 62-63 21.625510948330597 27.213409802861836 29.34264540491945 21.818433843888123 64-65 21.603030304355297 27.23312451820723 28.973984174241625 22.189861003195848 66-67 21.40939705899715 27.36810023319706 28.640736155864666 22.581766551941115 68-69 21.824386139531793 27.060545525470157 28.96824910918731 22.14681922581074 70-71 21.902187791883414 27.097100318688582 28.64078094445163 22.35993094497637 72-73 21.748546762036273 27.23416480028964 28.522161714973837 22.495126722700245 74-75 21.623513680501596 27.984762177948625 28.195917603471187 22.195806538078592 76-77 21.727116577723436 27.411218565958794 28.565950048363426 22.29571480795434 78-79 21.561378116131515 27.38802478475134 28.59158070567452 22.459016393442624 80-81 21.792564216933854 27.683030287128624 28.05809916126606 22.466306334671458 82-83 22.113516113516113 27.475490839127204 28.208491844855484 22.202501202501203 84-85 21.631310969063456 27.542459416638643 28.04449006062414 22.781739553673766 86-87 21.84634704863533 27.67771525418689 27.916329064054057 22.559608633123723 88-89 21.7572486265346 27.33611553725629 27.872952045462096 23.033683790747013 90-91 21.47686398589565 27.26893501137321 28.38176893807221 22.87243206465893 92-93 21.916336933287152 27.263134233846564 28.380291914622667 22.440236918243613 94-95 21.362122148981072 26.894316721867188 28.684011904772316 23.05954922437942 96-97 21.616013734253904 26.69158317798776 28.688227451278774 23.004175636479555 98-99 21.37906543887147 27.123115973466188 28.18670755429915 23.31111103336319 100-101 21.390502509112018 26.888416794713827 28.430625119463297 23.290455576710855 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 324.0 1 254.5 2 250.0 3 309.5 4 256.5 5 257.5 6 451.0 7 895.5 8 1455.5 9 1720.0 10 1562.0 11 1344.0 12 1390.0 13 1528.5 14 1652.0 15 1775.5 16 1844.0 17 1990.0 18 2152.5 19 2301.5 20 2562.5 21 2886.5 22 3307.0 23 3894.0 24 4517.0 25 5464.5 26 6996.0 27 8600.5 28 9778.5 29 11516.0 30 14336.5 31 17733.5 32 23085.0 33 26955.0 34 28724.5 35 31606.5 36 36900.0 37 40790.5 38 40467.0 39 39582.0 40 37603.0 41 35860.0 42 33991.5 43 33679.5 44 35172.0 45 36632.0 46 36793.0 47 37013.0 48 40629.0 49 39245.0 50 34280.5 51 32000.0 52 31301.5 53 32187.5 54 32532.0 55 31170.0 56 28828.5 57 26206.0 58 25163.0 59 23368.5 60 19617.0 61 16972.0 62 14747.5 63 11013.0 64 7512.0 65 5755.5 66 4482.5 67 3627.0 68 2822.5 69 2197.0 70 1785.5 71 1350.0 72 1095.0 73 1016.0 74 800.5 75 546.5 76 411.0 77 274.0 78 172.5 79 117.5 80 76.5 81 58.0 82 44.5 83 32.5 84 21.0 85 9.5 86 11.0 87 8.5 88 6.5 89 6.5 90 4.5 91 3.5 92 1.0 93 0.5 94 1.5 95 2.0 96 0.5 97 0.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02990775817741072 2 0.02037575337817748 3 0.018714211257210216 4 0.04844707026399281 5 0.05386894455346493 6 0.045911032289884884 7 0.04696042731365368 8 0.04083895634166902 9 0.0396146621472721 10-11 0.04700415210631071 12-13 0.04127620426823936 14-15 0.03712234896582121 16-17 0.04455556371751685 18-19 0.04774747358148028 20-21 0.010012977518460607 22-23 0.01766481623344142 24-25 0.005072075948215854 26-27 0.009969252725803575 28-29 0.005334424704158053 30-31 0.008307710604836312 32-33 0.0028421115227071593 34-35 0.006995966825125315 36-37 0.00843888498280741 38-39 0.00979435355517544 40-41 0.0017052669136242956 42-43 0.0020987900475375946 44-45 0.0018364412915953955 46-47 3.9352313391329897E-4 48-49 0.0011368446090828637 50-51 2.6234875594219933E-4 52-53 0.002798386730050126 54-55 0.005902847008699485 56-57 0.014210557613535797 58-59 0.008657508946092577 60-61 0.008395160190150379 62-63 0.004722277606959588 64-65 4.372479265703322E-5 66-67 0.005465599082129153 68-69 0.015566026185903825 70-71 0.006995966825125315 72-73 0.003060735485992325 74-75 0.003978956131790023 76-77 0.00787046267826598 78-79 0.006208920557298717 80-81 0.01101864774957237 82-83 0.005771672630728385 84-85 0.013948208857593597 86-87 0.0031919098639634253 88-89 0.005072075948215854 90-91 0.003410533827248591 92-93 0.014691530332763164 94-95 0.009532004799233242 96-97 0.008526334568121479 98-99 0.019238908769094617 100-101 0.03344946638263042 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1143516.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.224645168297606 #Duplication Level Percentage of deduplicated Percentage of total 1 75.06682320045513 33.19803619951458 2 12.738557322047697 11.267163550471576 3 4.560795417253239 6.05098677039667 4 2.253316747156508 3.986085343791166 5 1.2479917170922528 2.759599543068967 6 0.7904057385213927 2.0973247995056883 7 0.5265031771768641 1.6299091333439697 8 0.3842710118570507 1.3595399318272599 9 0.2931982629221992 1.166993022752594 >10 1.8008522506330369 15.481637512220797 >50 0.2003105275836583 6.235343203705763 >100 0.12781644037871032 10.43721491415422 >500 0.006968185701718966 2.088041652592031 >1k 0.0017918191804420199 1.1584382889796903 >5k 3.981820400982266E-4 1.083686133675004 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7058 0.617219173146681 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5250 0.4591103228988488 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3231 0.28254961014974866 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1706 0.14918899254579734 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1329 0.11622049888239432 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1283 0.11219781795794724 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1280 0.11193546920200503 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1165 0.1018787668908874 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.3117437797109966E-4 0.0 22-23 0.0 0.0 0.0 3.497983412562658E-4 0.0 24-25 0.0 0.0 0.0 0.001005670231111764 0.0 26-27 0.0 0.0 0.0 0.0018801660842524286 0.0 28-29 0.0 0.0 0.0 0.002929561108021226 0.0 30-31 0.0 0.0 0.0 0.005465599082129152 0.0 32-33 0.0 0.0 0.0 0.007957912263580046 0.0 34-35 0.0 0.0 0.0 0.01084374857894424 0.0 36-37 0.0 0.0 0.0 0.013598410516337332 0.0 38-39 0.0 0.0 0.0 0.017052669136242954 0.0 40-41 0.0 0.0 0.0 0.022999240937599474 0.0 42-43 0.0 0.0 0.0 0.034586310991713276 0.0 44-45 0.0 0.0 0.0 0.05006488759230304 0.0 46-47 0.0 0.0 0.0 0.06921634677608358 0.0 48-49 0.0 0.0 0.0 0.10402128173108202 0.0 50-51 0.0 0.0 0.0 0.1376456472843406 0.0 52-53 0.0 0.0 0.0 0.17183843514214056 0.0 54-55 0.0 0.0 0.0 0.2357640820067231 0.0 56-57 0.0 0.0 0.0 0.29885895781082206 0.0 58-59 0.0 0.0 0.0 0.38259193574904066 0.0 60-61 0.0 0.0 0.0 0.4818034902878491 0.0 62-63 0.0 0.0 0.0 0.6030960651184591 0.0 64-65 0.0 0.0 0.0 0.699071985000647 0.0 66-67 0.0 0.0 0.0 0.8351435397493345 0.0 68-69 0.0 0.0 0.0 1.0131908954487738 0.0 70-71 0.0 0.0 0.0 1.1232024737738695 0.0 72-73 8.744958531406644E-5 0.0 0.0 1.2914992007107902 0.0 74-75 8.744958531406644E-5 0.0 0.0 1.5010721319159503 0.0 76-77 8.744958531406644E-5 0.0 0.0 1.6501736748764337 0.0 78-79 8.744958531406644E-5 0.0 0.0 1.7924978749750768 0.0 80-81 8.744958531406644E-5 0.0 0.0 2.0129582795518384 0.0 82-83 8.744958531406644E-5 0.0 0.0 2.2944584946778184 0.0 84-85 8.744958531406644E-5 0.0 0.0 2.6387912368519553 0.0 86-87 8.744958531406644E-5 0.0 0.0 3.0966335407637495 0.0 88-89 8.744958531406644E-5 0.0 0.0 3.6009115744773137 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9875 0.0 30.371336 1 TAAGGTG 525 0.0 28.970924 5 ATACCGT 115 3.8466387E-6 28.930292 6 GGTATCA 6690 0.0 28.06355 1 GTACATG 7890 0.0 28.012228 1 TACATGG 7845 0.0 27.690638 2 AGGTAAG 430 0.0 27.636387 2 ACATGGG 7755 0.0 26.295727 3 ATCAACG 11815 0.0 25.386644 3 TCAACGC 11865 0.0 25.199537 4 CAACGCA 11860 0.0 25.16788 5 AACGCAG 12115 0.0 24.793983 6 TATCAAC 12155 0.0 24.715637 2 GATCGTG 175 1.4769648E-7 24.444218 7 ATGGGCG 215 1.6498234E-9 24.317892 5 CATGGGG 2465 0.0 24.104757 4 TAACACG 100 0.0016466492 23.767292 4 GAGTACT 5980 0.0 23.60728 12-13 GGTAAGG 485 0.0 23.522266 3 CATGGGA 4740 0.0 23.165588 4 >>END_MODULE