FastQCFastQC Report
Thu 26 May 2016
SRR936653_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936653_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143516
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70370.6153827318550855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61430.5372028025843101No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36650.3205027301760535No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25670.22448308550120857No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC19770.17288783016590936No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17690.15469831642058354No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA14980.13099947880047152No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14770.12916303750887612No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA13900.12155492358655236No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12420.10861238496007052No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA11890.103977556938425No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG11850.10362775859716873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAG6050.041.6156122
GGTATAC801.0775313E-535.6253433
GGTAAGG7650.033.5297363
TAAGGTG8200.033.0157245
GTAAGGT8350.030.7161734
GCGTATC650.00582881329.2463971
GTATCAA110450.028.7432921
GACATAT2700.028.16321
GGTATCA77100.028.0467661
GATTACG350.008390197527.144958-59
TAAGGTA3050.024.9160944
AAGGTAA9650.024.6245611
GAGTACT69400.024.53841412-13
GTAGATA3350.024.1026026
GTACTTT72050.023.53699514-15
GTGTGCA11950.023.4521089
ATCAACG136200.023.2968463
TCAACGC137100.023.2111784
GTACATG83550.023.151211
TAGATAT3300.023.0285117
Produced by FastQC (version 0.11.5)