##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936653_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1143516 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.874178411145973 31.0 31.0 34.0 30.0 34.0 2 32.00972351939107 31.0 31.0 34.0 30.0 34.0 3 32.09635545108245 33.0 31.0 34.0 30.0 34.0 4 35.86505392141431 37.0 35.0 37.0 35.0 37.0 5 35.64413615550635 37.0 35.0 37.0 33.0 37.0 6 35.76450438822019 37.0 35.0 37.0 33.0 37.0 7 35.70803469299948 37.0 35.0 37.0 33.0 37.0 8 35.68411548242438 37.0 35.0 37.0 33.0 37.0 9 37.30802717233515 39.0 37.0 39.0 34.0 39.0 10-11 37.1722179663424 39.0 37.0 39.0 33.0 39.0 12-13 37.08419033926941 39.0 37.0 39.0 33.0 39.0 14-15 38.14528874104079 40.0 37.0 41.0 33.0 41.0 16-17 38.08243522609216 40.0 37.0 41.0 33.0 41.0 18-19 38.272400211278196 40.0 38.0 41.0 33.5 41.0 20-21 38.313559670350045 40.0 38.0 41.0 34.0 41.0 22-23 38.213194655780946 40.0 38.0 41.0 33.0 41.0 24-25 38.081237166773356 40.0 37.0 41.0 33.0 41.0 26-27 37.84418101714361 40.0 37.0 41.0 33.0 41.0 28-29 37.746058209941964 40.0 37.0 41.0 32.5 41.0 30-31 37.6483827073692 39.5 37.0 41.0 33.0 41.0 32-33 37.44754642698484 39.0 36.0 41.0 32.0 41.0 34-35 37.256454653892035 39.0 36.0 41.0 32.0 41.0 36-37 36.97219846508488 39.0 35.5 41.0 31.0 41.0 38-39 36.74742417246458 39.0 35.0 41.0 31.0 41.0 40-41 36.48910246992609 39.0 35.0 41.0 30.0 41.0 42-43 36.17760529804568 39.0 35.0 40.0 30.0 41.0 44-45 35.81966452590082 39.0 35.0 40.0 29.5 41.0 46-47 35.335981306776645 38.0 35.0 40.0 27.5 41.0 48-49 34.85720007415725 38.0 34.0 40.0 25.0 41.0 50-51 34.4928234497812 38.0 34.0 40.0 24.0 41.0 52-53 34.20210867185068 38.0 33.5 40.0 23.0 41.0 54-55 33.924970004792236 38.0 33.0 40.0 23.0 41.0 56-57 33.58856675376646 37.5 33.0 40.0 21.5 41.0 58-59 33.244982142794676 37.0 33.0 40.0 20.5 41.0 60-61 32.87849011294989 36.0 32.0 39.0 19.5 41.0 62-63 32.478857751006544 36.0 31.5 39.0 18.0 40.0 64-65 32.04190540403457 35.0 31.0 39.0 15.5 40.0 66-67 31.581133976262684 35.0 31.0 38.0 12.0 40.0 68-69 31.289392540200573 35.0 30.5 38.0 9.0 40.0 70-71 30.85515462835675 35.0 30.0 37.0 7.5 39.5 72-73 30.41331603580536 34.0 29.5 37.0 6.5 39.0 74-75 29.8969625261037 34.0 29.0 36.0 2.0 39.0 76-77 28.49331972617786 32.5 27.0 35.0 2.0 37.0 78-79 29.267217074356633 34.0 29.0 35.0 2.0 37.0 80-81 29.077364024639795 34.0 29.0 35.0 2.0 37.0 82-83 28.744066545636443 34.0 29.0 35.0 2.0 36.5 84-85 28.320373742037717 33.5 28.0 35.0 2.0 36.0 86-87 27.966216476201467 33.0 27.0 35.0 2.0 36.0 88-89 27.551126525558015 33.0 26.5 35.0 2.0 35.0 90-91 27.07529540469919 33.0 26.0 35.0 2.0 35.0 92-93 26.596302981331263 32.5 25.0 34.5 2.0 35.0 94-95 25.99997114163685 32.0 24.5 34.0 2.0 35.0 96-97 25.248196352302898 32.0 21.5 34.0 2.0 35.0 98-99 24.320400414161234 31.5 10.0 34.0 2.0 35.0 100-101 21.16731248185421 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.234099911876271 1101 2 0.231104064607635 1101 3 0.28497110221455557 1101 4 0.14590999289728757 1101 5 0.12939769429488024 1101 6 0.13863915173664054 1101 7 0.15467204057593165 1101 8 0.18200135121193028 1101 9 0.26519222861454494 1101 10-11 0.29507748185109506 1101 12-13 0.27520533063528063 1101 14-15 0.4446026374894103 1101 16-17 0.3763671404842839 1101 18-19 0.35385542569053996 1101 20-21 0.2775127100196997 1101 22-23 0.24503702998361376 1101 24-25 0.14051617564732766 1101 26-27 0.20620995189929658 1101 28-29 0.05036288263107025 1101 30-31 0.329893368624262 1101 32-33 0.36647781263189216 1101 34-35 0.2760100653338853 1101 36-37 0.19571000889417434 1101 38-39 0.34326520313044995 1101 40-41 0.2515774209667683 1101 42-43 0.42179227012255893 1101 44-45 0.3416473847299173 1101 46-47 0.08429846692782661 1101 48-49 0.16501796098994248 1101 50-51 0.1893167653988499 1101 52-53 0.24857517369975568 1101 54-55 0.24971317382471625 1101 56-57 0.3573099212855979 1101 58-59 0.3584295804423121 1101 60-61 0.24364301847387537 1101 62-63 0.3336098786470316 1101 64-65 0.25418103171011097 1101 66-67 0.045201879576392656 1101 68-69 0.08041037540258955 1101 70-71 0.07428908478495799 1101 72-73 0.12638327554920892 1101 74-75 0.19567237184748976 1101 76-77 0.08864473544229412 1101 78-79 0.12420546749377692 1101 80-81 0.2837370345103203 1101 82-83 0.3543534327918536 1101 84-85 0.3933954447823673 1101 86-87 0.3239503673979023 1101 88-89 0.21216049358039868 1101 90-91 0.42564903605969207 1101 92-93 0.18654842026905172 1101 94-95 0.21969949913493636 1101 96-97 0.37761205117822527 1101 98-99 0.6747379554731943 1101 100-101 0.9312904569256766 1102 1 0.18132505213610273 1102 2 0.2591710971437138 1102 3 0.1129859981307817 1102 4 0.18171305891402767 1102 5 0.061538368208630345 1102 6 0.06588071822748987 1102 7 0.11505597062703288 1102 8 0.19032403580044388 1102 9 0.16113949666936378 1102 10-11 0.17362335408716234 1102 12-13 0.144114374147442 1102 14-15 0.2804572009730535 1102 16-17 0.3211214980237074 1102 18-19 0.23523616956413917 1102 20-21 0.044148606036813476 1102 22-23 0.0813903610433897 1102 24-25 0.1068289341904034 1102 26-27 -0.07808665342237475 1102 28-29 -0.19781473249749837 1102 30-31 0.022400761113026135 1102 32-33 0.17300119033613726 1102 34-35 0.18116501655510575 1102 36-37 0.17834445129484777 1102 38-39 0.21561220474979592 1102 40-41 0.23342159638869475 1102 42-43 0.3163439020398542 1102 44-45 0.2688645264470182 1102 46-47 0.3096211136236988 1102 48-49 0.23656793887067806 1102 50-51 0.1219064442655764 1102 52-53 0.2037589096309702 1102 54-55 0.4476668343221917 1102 56-57 0.5643409233998185 1102 58-59 0.4160110986953782 1102 60-61 0.6064182480096818 1102 62-63 0.3966586656488502 1102 64-65 0.4802971411495349 1102 66-67 0.36037276853315703 1102 68-69 0.4151852269250753 1102 70-71 0.4045814196518016 1102 72-73 0.468003226982713 1102 74-75 0.4984147032674713 1102 76-77 0.20334392951606617 1102 78-79 0.33369013215989085 1102 80-81 0.7090223364119375 1102 82-83 0.642923003906521 1102 84-85 0.541327168637249 1102 86-87 0.4761623616510029 1102 88-89 0.27426447554898203 1102 90-91 0.5279525433202394 1102 92-93 0.3603907202335783 1102 94-95 0.3301359995168305 1102 96-97 0.5520129251742389 1102 98-99 0.7429835125611959 1102 100-101 1.1625070069697543 1103 1 0.12265763698117027 1103 2 0.1730348440865228 1103 3 0.1419767518531998 1103 4 0.08512786624877577 1103 5 -0.05057780113622101 1103 6 0.12887873903986957 1103 7 0.051938520084647166 1103 8 0.14192107152666011 1103 9 0.20622956387995117 1103 10-11 0.06648582447950702 1103 12-13 0.10892465978896837 1103 14-15 0.027047296298420065 1103 16-17 0.16208245658437903 1103 18-19 0.14275875549196826 1103 20-21 0.10267382081282506 1103 22-23 0.18425885868709457 1103 24-25 0.08861009152489885 1103 26-27 0.08443606494698486 1103 28-29 0.27690357366363827 1103 30-31 0.12043374881269386 1103 32-33 0.1613886240979454 1103 34-35 0.13220533315077176 1103 36-37 0.25100187431727505 1103 38-39 0.32998452716545046 1103 40-41 0.3042643381049359 1103 42-43 0.2608820366032276 1103 44-45 0.43647764661609756 1103 46-47 0.2689943259534928 1103 48-49 0.2909286807848943 1103 50-51 0.36092785471674915 1103 52-53 0.413989855128726 1103 54-55 0.23099202753579107 1103 56-57 0.19456533511546326 1103 58-59 0.3786518991441028 1103 60-61 0.4033047567719663 1103 62-63 0.2689999019463798 1103 64-65 0.2669728727031 1103 66-67 0.5779031011286868 1103 68-69 0.49615695739680277 1103 70-71 0.3185297432905685 1103 72-73 0.43972881928679186 1103 74-75 0.4274699583382393 1103 76-77 0.26496711431196474 1103 78-79 0.2675583588793451 1103 80-81 0.39108877744780557 1103 82-83 0.4928049450625167 1103 84-85 0.5350819696426861 1103 86-87 0.3318392006016424 1103 88-89 0.5392850876333171 1103 90-91 0.4258903125308855 1103 92-93 0.6955051985092169 1103 94-95 0.839290729793845 1103 96-97 0.8519823009749885 1103 98-99 1.0323210865019057 1103 100-101 1.4436013736506261 1104 1 0.15859587485391557 1104 2 0.12743780593534382 1104 3 0.1846377621295474 1104 4 0.0945996364953885 1104 5 0.05930995139114259 1104 6 0.1383372551897395 1104 7 0.025819604328667367 1104 8 0.0325384473529553 1104 9 0.02798159838380343 1104 10-11 0.06514323356867635 1104 12-13 -0.024466778849962623 1104 14-15 0.019728580433863385 1104 16-17 0.16062082424910074 1104 18-19 -0.0011787715684761224 1104 20-21 -0.09957633210017747 1104 22-23 0.07751748145880555 1104 24-25 -0.13798170094932516 1104 26-27 -0.01585424886093989 1104 28-29 -0.08179450095791196 1104 30-31 -0.3509074577813678 1104 32-33 -0.20609937112664767 1104 34-35 -0.0551793684798767 1104 36-37 -0.06327141337614961 1104 38-39 -0.13848765837619226 1104 40-41 -0.05835989450740442 1104 42-43 -0.19553456616559828 1104 44-45 -0.24271823224341915 1104 46-47 -0.18767680456205227 1104 48-49 -0.0908111501321045 1104 50-51 -0.2747450066856487 1104 52-53 -0.2706073135998466 1104 54-55 -0.15062262580085672 1104 56-57 -0.18395055949188333 1104 58-59 -0.2171494484539096 1104 60-61 -0.15767611511197543 1104 62-63 -0.4245550726106586 1104 64-65 -0.239079586030023 1104 66-67 -0.4266749620648831 1104 68-69 -0.2595396042941438 1104 70-71 -0.3888161189276964 1104 72-73 -0.4924877663989804 1104 74-75 -0.3368979734237776 1104 76-77 -0.31469049800668714 1104 78-79 -0.29269991928585526 1104 80-81 -0.01004326968179825 1104 82-83 -0.060449770205124764 1104 84-85 -0.1701530549461161 1104 86-87 -0.1306855771958837 1104 88-89 0.0871063595749817 1104 90-91 0.17034052768905283 1104 92-93 0.0758300318757712 1104 94-95 0.2275999292169466 1104 96-97 0.39485439501427777 1104 98-99 0.48482404903797516 1104 100-101 1.2452199443091985 1105 1 0.08898191609729267 1105 2 0.1992151395128232 1105 3 0.09141582487289668 1105 4 0.047396289130638536 1105 5 0.06749908714866848 1105 6 0.002756263502227796 1105 7 0.10605909968009541 1105 8 0.0874550538040495 1105 9 0.1299781970717433 1105 10-11 0.12616674121833427 1105 12-13 -0.02112988557901474 1105 14-15 0.13741266192061374 1105 16-17 0.20640010519286278 1105 18-19 0.10949380444843371 1105 20-21 0.14452975988876204 1105 22-23 0.09907529773312973 1105 24-25 0.04157813505963048 1105 26-27 -0.03641014737421244 1105 28-29 0.00658369160986183 1105 30-31 -0.0876369131218766 1105 32-33 -0.04669658538409038 1105 34-35 0.1579708912237905 1105 36-37 -0.05299346411950978 1105 38-39 0.03489784229760318 1105 40-41 -0.006992078024751436 1105 42-43 -0.08201714677679917 1105 44-45 -0.055019973140630896 1105 46-47 -0.20513484462998832 1105 48-49 -0.244998206944544 1105 50-51 -0.21899451956996074 1105 52-53 -0.1698053407949942 1105 54-55 0.0813242141697188 1105 56-57 -0.2503375481026424 1105 58-59 -0.1713646850600128 1105 60-61 -0.12302894915491436 1105 62-63 -0.07215304673568568 1105 64-65 0.03808705084866659 1105 66-67 0.3070314702745556 1105 68-69 0.3088205181180008 1105 70-71 0.1930420698242905 1105 72-73 0.15975439123022994 1105 74-75 -0.18954204947194242 1105 76-77 -0.2994306463179299 1105 78-79 0.09464039064801355 1105 80-81 0.2565038612972259 1105 82-83 0.22248056649407033 1105 84-85 0.10805140974197158 1105 86-87 0.05691673902964567 1105 88-89 0.1004281651431782 1105 90-91 0.47405565519439463 1105 92-93 0.5393695455385803 1105 94-95 0.5598035123270542 1105 96-97 0.41865371102380067 1105 98-99 0.3735245548488244 1105 100-101 0.8149013372024072 1106 1 -0.05297301956635536 1106 2 0.0063893417064555535 1106 3 0.03782233303757465 1106 4 0.06013049218803701 1106 5 6.753075316794366E-4 1106 6 0.047655432822949706 1106 7 -0.07358660244612025 1106 8 -0.06735139436356263 1106 9 0.026976417586659807 1106 10-11 0.07241887948941184 1106 12-13 -0.039675283459395416 1106 14-15 0.006524209197195319 1106 16-17 -0.06871826937678094 1106 18-19 -0.10386318920809856 1106 20-21 -0.012850784368716006 1106 22-23 -0.11964174546184125 1106 24-25 -0.2033295552457659 1106 26-27 -0.11838756443030718 1106 28-29 -0.08962581255637048 1106 30-31 0.006142943153626845 1106 32-33 -0.10379622896180507 1106 34-35 -0.1380317747938804 1106 36-37 -0.03497362423231465 1106 38-39 0.05236265092270287 1106 40-41 0.084903823556715 1106 42-43 -0.02415178183645139 1106 44-45 0.12418683991187862 1106 46-47 0.15534628751705526 1106 48-49 0.1865009552559087 1106 50-51 0.04741056453974579 1106 52-53 -0.05612956893093468 1106 54-55 -0.03063539636291779 1106 56-57 -0.10901205640973188 1106 58-59 -0.025804672523662475 1106 60-61 -0.012909229320221982 1106 62-63 -0.18833054468277766 1106 64-65 0.056295986560165545 1106 66-67 0.046177574229400165 1106 68-69 0.04591837579070912 1106 70-71 0.12918750318931416 1106 72-73 -0.07070935460630068 1106 74-75 0.0872647640474753 1106 76-77 -0.22185641891152486 1106 78-79 -0.061056383692381644 1106 80-81 0.03267393548231823 1106 82-83 0.21798720615038292 1106 84-85 -0.180285288213053 1106 86-87 -0.2003495890154241 1106 88-89 -0.07793501206353781 1106 90-91 0.23207953379487734 1106 92-93 0.10355969889879546 1106 94-95 0.3206311450632775 1106 96-97 0.2889231784097319 1106 98-99 0.7303148166473257 1106 100-101 0.5459688473298279 1107 1 -0.06994001862287647 1107 2 0.09330763774366346 1107 3 0.045943557163553805 1107 4 0.003999523983132747 1107 5 -0.026230374023896275 1107 6 -6.132035098858069E-4 1107 7 -0.08606208152710337 1107 8 -0.1622970878038572 1107 9 -0.056260048342949176 1107 10-11 0.011613950458901456 1107 12-13 -0.023620488653222083 1107 14-15 0.16874366295191123 1107 16-17 0.19008102657529236 1107 18-19 -0.03843295024533688 1107 20-21 0.022690430756853175 1107 22-23 0.058385773568481625 1107 24-25 0.10987360219721154 1107 26-27 0.16741123980024497 1107 28-29 0.02846206833233822 1107 30-31 -0.0443931723158002 1107 32-33 0.09932733214458267 1107 34-35 0.1757838626457584 1107 36-37 0.0685893586929538 1107 38-39 0.16175765968855416 1107 40-41 0.20059130411807047 1107 42-43 -0.03572200679470683 1107 44-45 0.08915211533674494 1107 46-47 0.22881521784029246 1107 48-49 0.09552639098546933 1107 50-51 0.3400517694138756 1107 52-53 0.14355802283246533 1107 54-55 0.4078160109510449 1107 56-57 0.2192589777171321 1107 58-59 0.4454843502710162 1107 60-61 0.41855556220200896 1107 62-63 0.4792839456383078 1107 64-65 0.5316421463540237 1107 66-67 0.4189476692202696 1107 68-69 0.3410277447364969 1107 70-71 0.29611393087584403 1107 72-73 0.1928299283837802 1107 74-75 0.25947328872650743 1107 76-77 0.39029344669038224 1107 78-79 0.37116944680443 1107 80-81 0.6993032452130166 1107 82-83 0.6627371666215325 1107 84-85 0.472115372744085 1107 86-87 0.33021967856275225 1107 88-89 0.40439255092633886 1107 90-91 0.551914850520884 1107 92-93 0.6162376004467625 1107 94-95 0.7996061402416252 1107 96-97 0.5697048592633465 1107 98-99 1.039711041685507 1107 100-101 1.2548879461067806 1108 1 -0.07880269677778884 1108 2 -0.01580496621835792 1108 3 -0.04507885767404218 1108 4 -0.06217685643386517 1108 5 -0.2225233047289663 1108 6 -0.059720411625242775 1108 7 -0.06527212957466588 1108 8 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2316 92-93 -1.236803481174931 2316 94-95 -1.6012663285963065 2316 96-97 -1.5648952361413322 2316 98-99 -1.4047341709125192 2316 100-101 -1.5414242253635315 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 14.0 5 42.0 6 106.0 7 263.0 8 620.0 9 1096.0 10 1787.0 11 2968.0 12 4238.0 13 5593.0 14 7550.0 15 10338.0 16 13222.0 17 17309.0 18 14434.0 19 9040.0 20 7783.0 21 7910.0 22 8272.0 23 9198.0 24 10913.0 25 12710.0 26 15193.0 27 18599.0 28 22884.0 29 28502.0 30 34362.0 31 42397.0 32 52855.0 33 66950.0 34 89174.0 35 120420.0 36 168368.0 37 209957.0 38 122033.0 39 6407.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.31210413969276 20.40110928368968 15.160006298771739 21.126780277845818 2 15.744025130831444 21.926685416841572 39.094726863123725 23.234562589203257 3 18.977110839228416 26.906154703196506 30.33458770563558 23.782146751939496 4 12.670571609468409 17.777575670655562 37.45990408462381 32.09194863525223 5 12.5744858356668 39.433724003197185 33.52811441304199 14.46367574809402 6 30.264867767250774 37.87273264691119 17.075348443482106 14.787051142355931 7 27.85374231755393 34.720721004341 20.561059049457988 16.864477628647084 8 27.587545779857912 33.36464028487577 19.498896386233337 19.548917549032982 9 27.94018364669873 16.25806733712287 20.221775251421075 35.57997376475732 10-11 24.843869355280894 27.422151115837284 26.445599461659175 21.288380067222644 12-13 26.52756485990854 25.193375093900062 27.196124540119264 21.082935506072133 14-15 23.3580762769449 26.26207814490888 26.42959148782107 23.950254090325153 16-17 21.21559412391275 29.337366045114834 27.356748947512948 22.090290883459467 18-19 21.167609373196353 27.045052277362103 31.188107555993973 20.59923079344758 20-21 22.29404748162684 25.86614441774317 31.231176476761146 20.60863162386884 22-23 21.83303578104524 25.879965526785607 31.2963791318994 20.990619560269753 24-25 21.64319351467637 26.32218486145666 31.359591781408913 20.675029842458056 26-27 21.18847434029521 26.418013303412607 31.339060365590232 21.054451990701953 28-29 20.935139146210762 26.50794018656607 31.580284497297882 20.976636169925282 30-31 21.16220274402908 26.45148539441592 31.011035407927057 21.375276453627936 32-33 21.014602382834635 26.529804671922992 30.995695716354525 21.459897228887847 34-35 21.739143743457124 26.323814804546714 30.983086690006022 20.953954761990136 36-37 22.233844697971843 25.803659942879808 30.740782689196973 21.221712669951376 38-39 21.479468348175534 26.444496432842772 30.818576766579138 21.257458452402556 40-41 21.371340820327873 26.50604200809099 31.099944555896226 21.022672615684908 42-43 21.526109545268117 26.64788821076179 30.10426677883602 21.721735465134074 44-45 21.41484172361142 26.61026702089889 30.227913102336153 21.74697815315355 46-47 22.35431002028394 27.1494352284687 29.15487372055735 21.341381030690005 48-49 22.17325013325218 27.468357253138038 28.42764177091819 21.930750842691594 50-51 22.258912502426746 27.60667630952527 28.485289976615775 21.64912121143221 52-53 22.49330562886861 27.52680809236221 28.370666148956975 21.609220129812208 54-55 21.89468492316895 27.762021822799287 28.415201625209995 21.928091628821768 56-57 22.344798998864015 27.978244006782678 27.64269554639655 22.03426144795676 58-59 22.27286560303907 27.19499994752988 28.035702417473253 22.4964320319578 60-61 21.85871200195893 27.768260048273692 28.490441459404625 21.88258649036275 62-63 21.893988421107867 27.785318244625962 28.803805992339043 21.51688734192713 64-65 21.7404993407862 27.80461034233202 28.463037018735665 21.99185329814611 66-67 21.81703936813688 27.803875115820148 28.066495521766754 22.312589994276223 68-69 22.181313979556275 27.59172543825627 28.17713113603599 22.049829446151463 70-71 22.206214082799928 27.748071626488667 27.95073612906865 22.094978161642757 72-73 22.006074296003437 27.689729927320077 27.902540168096916 22.40165560857957 74-75 21.988537256104042 28.4530018317477 27.41788291244823 22.140577999700028 76-77 22.068097327371422 27.860497900708452 27.975759982019817 22.095644789900312 78-79 21.982087082681627 27.86082617351559 27.768126410993144 22.38896033280964 80-81 22.32179322250606 28.38378396814583 27.097688508834146 22.196734300513967 82-83 22.466363203071367 28.351414341497115 27.152120965661393 22.03010148977013 84-85 21.913282116694006 28.36204849649327 27.206066587461468 22.51860279935126 86-87 22.034202168758043 28.488878763723086 26.895914218662476 22.58100484885639 88-89 21.850757651289747 28.384548916304155 26.87187008593653 22.89282334646957 90-91 21.669802851121833 28.236024154815414 27.324468054867413 22.769704939195336 92-93 22.06846493893774 28.07495376058697 27.275744312442885 22.58083698803241 94-95 21.549313572836226 27.811001986456418 27.551927492373153 23.087756948334206 96-97 22.11259377932312 27.516180458570943 27.418629848999878 22.952595913106062 98-99 21.48654918033145 27.81652157601288 27.09298497001042 23.603944273645244 100-101 21.130240254106848 28.08140409425461 27.180626101922595 23.60772954971595 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 523.0 1 357.5 2 241.0 3 333.5 4 318.5 5 319.5 6 516.5 7 919.0 8 1354.0 9 1586.5 10 1562.0 11 1420.5 12 1458.0 13 1602.0 14 1703.0 15 1860.0 16 2042.5 17 2213.0 18 2417.0 19 2558.5 20 2714.5 21 3072.0 22 3524.5 23 4047.0 24 4604.5 25 5505.5 26 7092.0 27 8667.0 28 9905.0 29 12001.5 30 14819.5 31 17740.0 32 22557.0 33 25964.5 34 28036.0 35 31293.0 36 36458.5 37 40218.5 38 40189.5 39 39711.5 40 37970.5 41 35474.5 42 32770.5 43 33203.0 44 34807.0 45 35790.0 46 35296.0 47 35499.5 48 40409.5 49 39248.0 50 33900.0 51 31424.0 52 30631.0 53 32419.5 54 34152.0 55 32748.0 56 29058.0 57 26445.0 58 26116.5 59 24246.5 60 20268.0 61 17269.5 62 14754.0 63 10930.5 64 7399.0 65 5845.5 66 4659.0 67 3674.0 68 2915.5 69 2377.0 70 1942.0 71 1460.5 72 1197.5 73 1070.0 74 830.5 75 632.5 76 480.0 77 313.5 78 221.0 79 157.0 80 112.0 81 84.5 82 65.0 83 39.5 84 19.5 85 12.0 86 7.5 87 2.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03812801919693297 2 0.005246975118843986 3 0.0042850296803892554 4 6.995966825125315E-4 5 8.744958531406643E-4 6 1.748991706281329E-4 7 0.0 8 0.0 9 0.001399193365025063 10-11 2.1862396328516608E-4 12-13 0.0011368446090828637 14-15 0.0021862396328516612 16-17 0.002973285900678259 18-19 0.0 20-21 0.0 22-23 0.0017052669136242956 24-25 9.619454384547309E-4 26-27 0.0043724792657033225 28-29 0.00529069991150102 30-31 0.0013117437797109965 32-33 3.497983412562658E-4 34-35 0.008482609775464445 36-37 0.012024317980684136 38-39 0.008613784153435545 40-41 0.0019238908769094619 42-43 6.12147097198465E-4 44-45 8.744958531406643E-4 46-47 4.372479265703322E-5 48-49 6.558718898554982E-4 50-51 8.744958531406643E-4 52-53 0.0015740925356531959 54-55 0.0028858363153641924 56-57 0.0013117437797109965 58-59 6.995966825125315E-4 60-61 0.0031481850713063917 62-63 0.00489717677758772 64-65 0.009488280006576208 66-67 0.003541708205219691 68-69 0.0031044602786493587 70-71 4.372479265703322E-5 72-73 7.870462678265979E-4 74-75 0.006340094935269817 76-77 0.003060735485992325 78-79 0.0034542586199056246 80-81 0.004809727192273654 82-83 0.004459928851017389 84-85 0.006340094935269817 86-87 0.004416204058360355 88-89 0.009969252725803575 90-91 0.012374116321940402 92-93 9.619454384547309E-4 94-95 0.001967615669566495 96-97 6.12147097198465E-4 98-99 0.0031044602786493587 100-101 0.005246975118843986 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1143516.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.68471055249997 #Duplication Level Percentage of deduplicated Percentage of total 1 73.33505693522862 30.569506216961102 2 13.36680494979543 11.143827906878927 3 4.924086350719667 6.157773427957956 4 2.4146009433781974 4.026077656980542 5 1.3802594960468575 2.876785879002637 6 0.868431583658126 2.172019151966289 7 0.5955460262216418 1.7377614606618483 8 0.4353376002301444 1.451753748657081 9 0.32745518271996515 1.2284887059537963 >10 1.971810016027819 15.789488546507016 >50 0.2328381324688812 6.795679931924596 >100 0.13556363636988403 10.540565573355364 >500 0.008630604009096533 2.319952799341162 >1k 0.0031575380521084878 2.0338086154215156 >5k 4.210050736144651E-4 1.1565103784302173 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7037 0.6153827318550855 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6143 0.5372028025843101 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3665 0.3205027301760535 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2567 0.22448308550120857 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1977 0.17288783016590936 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1769 0.15469831642058354 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1498 0.13099947880047152 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1477 0.12916303750887612 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1390 0.12155492358655236 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1242 0.10861238496007052 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 1189 0.103977556938425 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 1185 0.10362775859716873 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.372479265703322E-5 0.0 22-23 0.0 0.0 0.0 2.186239632851661E-4 0.0 24-25 0.0 0.0 0.0 7.870462678265979E-4 0.0 26-27 0.0 0.0 0.0 0.0017052669136242956 0.0 28-29 0.0 0.0 0.0 0.002754661937393093 0.0 30-31 0.0 0.0 0.0 0.00529069991150102 0.0 32-33 0.0 0.0 0.0 0.007826737885608946 0.0 34-35 0.0 0.0 0.0 0.010625124615659072 0.0 36-37 0.0 0.0 0.0 0.013510960931023264 0.0 38-39 0.0 0.0 0.0 0.01700894434358592 0.0 40-41 0.0 0.0 0.0 0.023042965730256507 0.0 42-43 0.0 0.0 0.0 0.03511100850359768 0.0 44-45 0.0 0.0 0.0 0.050851933860129636 0.0 46-47 0.0 0.0 0.0 0.06995966825125316 0.0 48-49 0.0 0.0 0.0 0.1046334288282805 0.0 50-51 0.0 0.0 0.0 0.138214069588882 0.0 52-53 0.0 0.0 0.0 0.17218823348339682 0.0 54-55 0.0 0.0 0.0 0.23628877951860752 0.0 56-57 0.0 0.0 0.0 0.2996460040786486 0.0 58-59 0.0 0.0 0.0 0.383029183675611 0.0 60-61 0.0 0.0 0.0 0.4822407382144194 0.0 62-63 0.0 0.0 0.0 0.6035333130450296 0.0 64-65 0.0 0.0 0.0 0.6995092329272175 0.0 66-67 0.0 0.0 0.0 0.8353621637126196 0.0 68-69 0.0 0.0 0.0 1.0133220698267449 0.0 70-71 0.0 0.0 0.0 1.1238146208710678 0.0 72-73 0.0 0.0 0.0 1.2935105411730137 0.0 74-75 0.0 0.0 0.0 1.504307766572571 0.0 76-77 0.0 0.0 0.0 1.6563388706410755 0.0 78-79 0.0 0.0 0.0 1.7996250161781733 0.0 80-81 0.0 0.0 0.0 2.0197793472063355 0.0 82-83 0.0 0.0 0.0 2.299749194589319 0.0 84-85 0.0 0.0 0.0 2.6430762665323444 4.372479265703322E-5 86-87 0.0 0.0 0.0 3.098338807677374 8.744958531406644E-5 88-89 0.0 0.0 0.0 3.6011739232332562 8.744958531406644E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTAAG 605 0.0 41.615612 2 GGTATAC 80 1.0775313E-5 35.625343 3 GGTAAGG 765 0.0 33.529736 3 TAAGGTG 820 0.0 33.015724 5 GTAAGGT 835 0.0 30.716173 4 GCGTATC 65 0.005828813 29.246397 1 GTATCAA 11045 0.0 28.743292 1 GACATAT 270 0.0 28.1632 1 GGTATCA 7710 0.0 28.046766 1 GATTACG 35 0.0083901975 27.1449 58-59 TAAGGTA 305 0.0 24.916094 4 AAGGTAA 965 0.0 24.624561 1 GAGTACT 6940 0.0 24.538414 12-13 GTAGATA 335 0.0 24.102602 6 GTACTTT 7205 0.0 23.536995 14-15 GTGTGCA 1195 0.0 23.452108 9 ATCAACG 13620 0.0 23.296846 3 TCAACGC 13710 0.0 23.211178 4 GTACATG 8355 0.0 23.15121 1 TAGATAT 330 0.0 23.028511 7 >>END_MODULE