FastQCFastQC Report
Thu 26 May 2016
SRR936652_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936652_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196130
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69830.5837994197955072No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52240.43674182572128445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31730.2652721694130237No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18060.15098693285846856No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13790.11528847198883063No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12430.10391847040037454No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG12250.10241361724896124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98500.033.204271
GGTATCA63750.032.0649031
CGTATAG1205.348469E-627.7305531
ATCAACG119200.027.2797093
GTACATG84150.027.1726911
TCAACGC120300.027.0302684
TACATGG83700.026.8076212
TATCAAC121950.026.7803752
CAACGCA121550.026.7142985
AACGCAG124050.026.1759246
ACATGGG84350.025.7003843
GAGTACT64350.023.6757112-13
CATGGGA51950.023.6098944
ACGCAGA137550.023.537737
CGCAGAG138500.023.3782378
GTACTTT67150.022.68231814-15
GCAGAGT143800.022.084919
GTATAGG5200.021.9406551
ATGGGAT21150.021.8041635
AGTACTT69050.021.13480412-13
Produced by FastQC (version 0.11.5)