##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936652_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1196130 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.437445762584336 31.0 31.0 34.0 30.0 34.0 2 31.487931077725666 31.0 31.0 34.0 28.0 34.0 3 31.418853301898622 31.0 31.0 34.0 28.0 34.0 4 35.11667628100624 37.0 35.0 37.0 32.0 37.0 5 35.16223821825387 37.0 35.0 37.0 32.0 37.0 6 35.23205253609557 37.0 35.0 37.0 32.0 37.0 7 35.09960539406252 37.0 35.0 37.0 32.0 37.0 8 35.07813699179855 37.0 35.0 37.0 32.0 37.0 9 36.572073269627886 39.0 37.0 39.0 32.0 39.0 10-11 36.46519943484404 39.0 37.0 39.0 32.0 39.0 12-13 36.38688980294784 39.0 37.0 39.0 32.0 39.0 14-15 37.34202218822369 40.0 37.0 41.0 32.0 41.0 16-17 37.275792346985696 39.0 37.0 41.0 32.0 41.0 18-19 37.218423582720945 39.5 37.0 41.0 31.0 41.0 20-21 37.24558827217777 39.5 37.0 41.0 31.0 41.0 22-23 37.22656107613721 39.0 36.5 41.0 31.0 41.0 24-25 37.32276717413659 40.0 37.0 41.0 31.5 41.0 26-27 37.24426483743406 39.5 36.5 41.0 31.0 41.0 28-29 37.12666223571017 39.0 36.0 41.0 31.0 41.0 30-31 36.89297526188625 39.0 36.0 41.0 30.5 41.0 32-33 36.6842207786779 39.0 35.0 41.0 30.0 41.0 34-35 36.474474346433915 39.0 35.0 40.5 30.0 41.0 36-37 36.24099428991832 39.0 35.0 40.0 30.0 41.0 38-39 36.009908203957764 38.0 35.0 40.0 29.5 41.0 40-41 35.75912777039285 38.0 35.0 40.0 28.5 41.0 42-43 35.43516549204518 38.0 35.0 40.0 27.0 41.0 44-45 35.18624104403368 38.0 34.5 40.0 26.0 41.0 46-47 34.91837216690493 38.0 34.0 40.0 24.5 41.0 48-49 34.59174044627257 38.0 34.0 40.0 23.5 41.0 50-51 33.56752234288915 37.5 32.5 39.5 22.5 40.5 52-53 33.65587436148245 37.5 33.0 39.5 22.5 40.5 54-55 34.08858694289083 38.0 34.0 40.0 22.5 41.0 56-57 33.93331285060988 38.0 33.5 40.0 21.5 41.0 58-59 33.702573298888915 38.0 33.0 40.0 20.5 41.0 60-61 33.41332296656718 37.0 33.0 40.0 19.5 41.0 62-63 33.0789404161755 37.0 33.0 40.0 18.0 41.0 64-65 32.71937540233921 36.0 32.0 39.0 17.0 41.0 66-67 32.34832250675094 36.0 32.0 39.0 13.5 41.0 68-69 31.921857574009515 35.0 31.0 39.0 10.0 40.0 70-71 31.51786302492204 35.0 31.0 38.0 8.0 40.0 72-73 31.115183132268232 35.0 31.0 37.0 7.0 39.5 74-75 30.717585463118557 35.0 30.5 37.0 3.5 39.0 76-77 30.276186116893648 34.5 30.0 36.0 2.0 39.0 78-79 29.823984015115414 34.0 29.5 36.0 2.0 38.5 80-81 29.409153687308233 34.0 29.0 35.0 2.0 37.0 82-83 29.03052427411736 34.0 29.0 35.0 2.0 37.0 84-85 28.669967311245433 34.0 29.0 35.0 2.0 36.0 86-87 28.346606556143563 34.0 29.0 35.0 2.0 36.0 88-89 27.997003670169633 34.0 27.0 35.0 2.0 36.0 90-91 27.658127461061923 34.0 27.0 35.0 2.0 35.0 92-93 27.260551110665226 33.5 26.0 35.0 2.0 35.0 94-95 26.795446147157918 33.0 25.0 35.0 2.0 35.0 96-97 26.22639219817244 33.0 24.5 35.0 2.0 35.0 98-99 25.53253743322214 33.0 21.5 35.0 2.0 35.0 100-101 23.049007632949596 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7173333424916173 1101 2 0.5795869340967066 1101 3 0.7238857963609426 1101 4 0.7213403945557673 1101 5 0.7369024077364443 1101 6 0.7226763797354963 1101 7 0.6860846447137234 1101 8 0.7447040913713252 1101 9 0.9692999945282139 1101 10-11 0.9147028786424798 1101 12-13 0.9653646612932718 1101 14-15 1.0987997300017085 1101 16-17 1.099717425583215 1101 18-19 1.275644322848379 1101 20-21 1.2230899211872668 1101 22-23 1.128872758299245 1101 24-25 0.8504112056628301 1101 26-27 0.9615089607289207 1101 28-29 0.9613641739992076 1101 30-31 0.5936780460206492 1101 32-33 0.6174341635195404 1101 34-35 0.818218808697246 1101 36-37 0.856050125058438 1101 38-39 0.7273124914868774 1101 40-41 0.6211096998453556 1101 42-43 0.8656260582655761 1101 44-45 0.8557538491081544 1101 46-47 1.0487356939788341 1101 48-49 0.9603524523223541 1101 50-51 0.906848394863836 1101 52-53 0.9765964647272725 1101 54-55 1.0413037166147063 1101 56-57 1.1169971500687979 1101 58-59 1.1151455419740586 1101 60-61 1.0357320806305381 1101 62-63 1.1416877373304857 1101 64-65 1.1234220589788464 1101 66-67 0.9810325475019326 1101 68-69 0.9567382445197232 1101 70-71 0.9920749749639555 1101 72-73 0.9813554470307864 1101 74-75 0.736974316336191 1101 76-77 0.776998604063305 1101 78-79 0.8794329375936414 1101 80-81 0.9571396919291466 1101 82-83 1.036003939399805 1101 84-85 1.232518199455093 1101 86-87 0.9224467034055515 1101 88-89 0.9837419512522594 1101 90-91 0.9409153611643859 1101 92-93 0.8454682635077297 1101 94-95 0.8792835220143438 1101 96-97 1.0870108937400182 1101 98-99 0.8729143394182302 1101 100-101 1.3923139053265352 1102 1 0.28290314682794815 1102 2 0.40827634005475133 1102 3 0.4655846329553377 1102 4 0.3944414874519424 1102 5 0.43207735926120705 1102 6 0.4189252780256396 1102 7 0.4101468692862582 1102 8 0.34706703866552857 1102 9 0.44857374739265765 1102 10-11 0.43320829905496083 1102 12-13 0.433014273491402 1102 14-15 0.694787082372585 1102 16-17 0.6677228900640912 1102 18-19 0.5731575433666336 1102 20-21 0.5169994487740794 1102 22-23 0.3662786722777369 1102 24-25 0.5471367036356867 1102 26-27 0.3543478466799215 1102 28-29 0.2764252087250938 1102 30-31 0.32402883043807407 1102 32-33 0.46861167101116763 1102 34-35 0.2285856780785238 1102 36-37 0.272259582135824 1102 38-39 0.3263372579243864 1102 40-41 0.38930620081486467 1102 42-43 0.3905676676433316 1102 44-45 0.6393487283607513 1102 46-47 0.6326624082393693 1102 48-49 0.9445814757667392 1102 50-51 0.845274712508818 1102 52-53 0.5628458037013573 1102 54-55 0.9084941361458192 1102 56-57 0.6782526586180637 1102 58-59 0.6205646324023988 1102 60-61 0.8661520524267701 1102 62-63 1.0091368383352517 1102 64-65 1.0282396158270757 1102 66-67 0.7688742978985417 1102 68-69 0.8101200530866244 1102 70-71 0.5158340089847613 1102 72-73 0.8322502557739604 1102 74-75 0.5527089364666331 1102 76-77 0.6565513098627065 1102 78-79 0.6794668702571371 1102 80-81 0.9825775114250099 1102 82-83 0.8088950463978648 1102 84-85 0.6904040182458537 1102 86-87 0.40424116841524693 1102 88-89 0.5363761395124378 1102 90-91 0.7492949663115738 1102 92-93 0.5842581454044335 1102 94-95 0.7237084294707152 1102 96-97 0.7490814472390461 1102 98-99 0.8907611576801706 1102 100-101 1.3691352520566582 1103 1 0.26955690527207565 1103 2 0.33139668771621444 1103 3 0.3322646381186267 1103 4 0.2536566675216463 1103 5 0.29594165081493173 1103 6 0.31301589374785976 1103 7 0.39009996941103964 1103 8 0.2456036765038334 1103 9 0.35076642412774106 1103 10-11 0.22409401825809283 1103 12-13 0.3203065586770677 1103 14-15 0.6258481382651908 1103 16-17 0.6728801552420123 1103 18-19 0.7317604434139611 1103 20-21 0.6637622816986521 1103 22-23 0.3357799846137013 1103 24-25 0.3448999238069277 1103 26-27 0.30056788884677843 1103 28-29 0.39147963839680244 1103 30-31 0.3513615613876837 1103 32-33 0.44438573408934445 1103 34-35 0.5777623486176608 1103 36-37 0.6518204874324951 1103 38-39 0.33735291990107186 1103 40-41 0.5524157916242061 1103 42-43 0.6532101959338519 1103 44-45 0.7340055847782949 1103 46-47 0.927257305188725 1103 48-49 1.0951182426872634 1103 50-51 0.8835385565774985 1103 52-53 0.9717122466254935 1103 54-55 0.9956868128808978 1103 56-57 1.0407917059912393 1103 58-59 0.8733211833253378 1103 60-61 0.9426991028979188 1103 62-63 0.8175222348328148 1103 64-65 0.7399447920243176 1103 66-67 0.795461363859566 1103 68-69 0.9322589455611272 1103 70-71 0.8317371542883194 1103 72-73 0.900728806610843 1103 74-75 0.9068212810301226 1103 76-77 0.9540210831082625 1103 78-79 0.9235179007635708 1103 80-81 0.8197174795847246 1103 82-83 0.7129338140928851 1103 84-85 0.7703087125361172 1103 86-87 0.622196936239348 1103 88-89 0.699729695727747 1103 90-91 0.7586964550599085 1103 92-93 0.8505059402497501 1103 94-95 0.8447447446034531 1103 96-97 0.8290601486718607 1103 98-99 1.0848883466995787 1103 100-101 1.6429965316917858 1104 1 0.13324194464215466 1104 2 0.33686650398917806 1104 3 0.4799234307695457 1104 4 0.38850706122243395 1104 5 0.3415636980590264 1104 6 0.25749358403657396 1104 7 0.31295561245566006 1104 8 0.17989763975842266 1104 9 0.3702677364637026 1104 10-11 0.4897055668145214 1104 12-13 0.5375716505405777 1104 14-15 0.5294045687113851 1104 16-17 0.48505075891655025 1104 18-19 0.38933787123548314 1104 20-21 0.5297754050582313 1104 22-23 0.36461200561107177 1104 24-25 0.42932249598541006 1104 26-27 0.15933691771816427 1104 28-29 0.13114105571963108 1104 30-31 0.19161352989161884 1104 32-33 0.238272873196955 1104 34-35 0.1556731097725148 1104 36-37 0.18143991000467707 1104 38-39 0.024801738798707618 1104 40-41 0.1462898073722343 1104 42-43 0.20202121955589547 1104 44-45 0.30829954803288473 1104 46-47 0.43703945741969363 1104 48-49 0.7048820222148251 1104 50-51 0.5795096851864159 1104 52-53 0.4173867873079118 1104 54-55 0.5428547918835207 1104 56-57 0.6600777952300803 1104 58-59 0.6155810258450245 1104 60-61 0.6804471343939724 1104 62-63 0.6641259093734959 1104 64-65 0.8502177579035788 1104 66-67 0.748852439975046 1104 68-69 0.6271145886057461 1104 70-71 0.49277389969514473 1104 72-73 0.6142721136974529 1104 74-75 0.653162488379202 1104 76-77 0.7849502169665286 1104 78-79 0.6093630451205243 1104 80-81 0.8282660360151688 1104 82-83 0.7581464125180801 1104 84-85 0.531316586551867 1104 86-87 0.39365100448081947 1104 88-89 0.6098898007146225 1104 90-91 0.6547594471858922 1104 92-93 0.5559521344754721 1104 94-95 0.7566975005089667 1104 96-97 0.7192071302991572 1104 98-99 0.29492509210639994 1104 100-101 1.042211754788898 1105 1 0.2574105167112002 1105 2 0.2258591065446609 1105 3 0.333219104385325 1105 4 0.41452858106103463 1105 5 0.3857484100070181 1105 6 0.3063333060719913 1105 7 0.4296787686986505 1105 8 0.43116506858604 1105 9 0.3717629169165093 1105 10-11 0.4458335650212035 1105 12-13 0.646108301515703 1105 14-15 0.7483229726566876 1105 16-17 0.7821597030994667 1105 18-19 0.5445840043894847 1105 20-21 0.6043089177235572 1105 22-23 0.5856868767162027 1105 24-25 0.5175897006480383 1105 26-27 0.45622752520527854 1105 28-29 0.4943351825970197 1105 30-31 0.16087692374950535 1105 32-33 0.2682266952099539 1105 34-35 0.23767736890723512 1105 36-37 0.3089987533133538 1105 38-39 0.19684096767115733 1105 40-41 0.29347743346503563 1105 42-43 0.28340835248573626 1105 44-45 0.34329484056818416 1105 46-47 0.1962667607149271 1105 48-49 0.12338796258693918 1105 50-51 0.15357043389746394 1105 52-53 0.38818100385128673 1105 54-55 0.41723811400860455 1105 56-57 0.32442704427738533 1105 58-59 0.32509548448658876 1105 60-61 0.44415661657286165 1105 62-63 0.4928053534443393 1105 64-65 0.6272780582846593 1105 66-67 0.3154177840682948 1105 68-69 0.2524923066061966 1105 70-71 0.4217404991123175 1105 72-73 0.1858009188980816 1105 74-75 0.21871707255315087 1105 76-77 0.4259322837676933 1105 78-79 0.4518160825560784 1105 80-81 0.3907482148988315 1105 82-83 0.48454609868710463 1105 84-85 0.381688924592666 1105 86-87 0.5241163709905692 1105 88-89 0.4271839051755073 1105 90-91 0.5559251051101235 1105 92-93 0.35691604391281473 1105 94-95 0.42811668903171807 1105 96-97 0.6530670271832655 1105 98-99 0.6229694767735161 1105 100-101 0.9446930370126161 1106 1 0.1924604906696139 1106 2 0.12317035274002208 1106 3 0.056465406911790694 1106 4 0.11053407000028415 1106 5 0.14530081262125805 1106 6 0.06993157638408576 1106 7 0.2155047909386667 1106 8 0.43705541603571163 1106 9 0.29761637477115244 1106 10-11 0.30365582564864724 1106 12-13 0.42678299422728116 1106 14-15 0.3799526218286431 1106 16-17 0.10919863197528912 1106 18-19 0.2924526585413574 1106 20-21 0.012494738841709818 1106 22-23 -0.07674631084177719 1106 24-25 -0.1677110399137618 1106 26-27 -0.3345575229750608 1106 28-29 -0.11244053329679815 1106 30-31 -0.16393632381083734 1106 32-33 0.006090113800148345 1106 34-35 0.09616894591926695 1106 36-37 -0.13028189958343717 1106 38-39 0.022263138753693568 1106 40-41 -0.0060505032287139215 1106 42-43 0.07541397220050783 1106 44-45 -0.13559376729460126 1106 46-47 0.01001244864184514 1106 48-49 0.11027590022517586 1106 50-51 -0.011024863541919672 1106 52-53 0.020609609725198652 1106 54-55 0.2722970606208577 1106 56-57 0.22816377317291625 1106 58-59 0.11978359167891739 1106 60-61 0.2050710371623765 1106 62-63 0.11318217766068983 1106 64-65 -0.0219951612951661 1106 66-67 -0.059402342829365296 1106 68-69 0.1454648024252414 1106 70-71 -0.10523195220672221 1106 72-73 -0.002052441783078507 1106 74-75 0.29805490341408714 1106 76-77 0.09958514831922116 1106 78-79 0.2936369591425816 1106 80-81 0.4287339630914708 1106 82-83 0.5018211818181051 1106 84-85 0.39708138511140234 1106 86-87 0.5141277094099195 1106 88-89 0.4927137935122836 1106 90-91 0.7516084231030646 1106 92-93 0.6031724810881194 1106 94-95 0.8602453285530807 1106 96-97 0.865254170587253 1106 98-99 0.7077042953474582 1106 100-101 1.1534309760358035 1107 1 0.1015779026474064 1107 2 0.18638356435663184 1107 3 0.29420689533647604 1107 4 0.22412910846652778 1107 5 0.3261253778490527 1107 6 0.29661169427279077 1107 7 0.38747529749502974 1107 8 0.2948162749290262 1107 9 0.3632336157287881 1107 10-11 0.4731097662895962 1107 12-13 0.3396635140576407 1107 14-15 0.2518323902336945 1107 16-17 0.16566230747298505 1107 18-19 0.5923247478759066 1107 20-21 0.256938134717025 1107 22-23 0.35119993212025946 1107 24-25 0.36360071120851245 1107 26-27 0.3028644767732871 1107 28-29 0.24285759115270622 1107 30-31 -0.06432085331047688 1107 32-33 0.14254846374813468 1107 34-35 0.10302481580925615 1107 36-37 0.1692089388760678 1107 38-39 0.12081748683020521 1107 40-41 0.043557523907672646 1107 42-43 0.2168584898971062 1107 44-45 0.33768012546070736 1107 46-47 0.13001321070053962 1107 48-49 0.2893439644720388 1107 50-51 0.04429971143313338 1107 52-53 0.10491434636303154 1107 54-55 0.12068681288089778 1107 56-57 0.4344924933928098 1107 58-59 0.31885924106812524 1107 60-61 0.326229286624951 1107 62-63 0.4379290589797975 1107 64-65 0.46336998887470315 1107 66-67 0.4818130698963117 1107 68-69 0.35811196393383327 1107 70-71 0.37077914903897735 1107 72-73 0.24521699558722077 1107 74-75 0.2611519896915375 1107 76-77 0.44549089938122677 1107 78-79 0.3641346986638325 1107 80-81 0.32332640346923824 1107 82-83 0.2670676723606036 1107 84-85 0.4569885025623641 1107 86-87 0.5385355189165146 1107 88-89 0.5363438689560915 1107 90-91 0.32726600886568136 1107 92-93 0.4144823182025057 1107 94-95 0.34015156875043573 1107 96-97 0.6505824583831448 1107 98-99 0.8063450396129639 1107 100-101 1.257825928017244 1108 1 0.17750086266719833 1108 2 0.12920952312454403 1108 3 0.16829007381230454 1108 4 0.2460421427249102 1108 5 0.13819114903941454 1108 6 0.31100693154424874 1108 7 0.42157975608901666 1108 8 0.37127774842839756 1108 9 -0.13587211706807523 1108 10-11 0.1485316948561035 1108 12-13 0.06208050950585431 1108 14-15 0.3608562578207639 1108 16-17 0.562834600163896 1108 18-19 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86-87 -4.743022262845912 2316 88-89 -5.680780166282073 2316 90-91 -5.167187747533578 2316 92-93 -4.662529713709347 2316 94-95 -4.045536822923378 2316 96-97 -5.77707339775365 2316 98-99 -7.464520452448053 2316 100-101 -8.388029169303511 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2503.0 3 888.0 4 908.0 5 960.0 6 1188.0 7 1337.0 8 1712.0 9 2265.0 10 2900.0 11 3919.0 12 5352.0 13 6693.0 14 7928.0 15 10031.0 16 13021.0 17 16811.0 18 12639.0 19 8320.0 20 8107.0 21 8482.0 22 9568.0 23 10494.0 24 12092.0 25 13673.0 26 15994.0 27 18858.0 28 22472.0 29 26889.0 30 32477.0 31 40150.0 32 50622.0 33 64686.0 34 85241.0 35 117796.0 36 162809.0 37 208657.0 38 163040.0 39 24643.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.53698900620806 19.30389384381118 14.588778634895668 21.570338515085087 2 15.364609248971597 20.628757462658484 40.456368901819474 23.55026438655045 3 18.622320998555146 26.684827687697332 30.148131053673676 24.54472026007385 4 12.013800681170315 17.015795023426207 38.38460785879555 32.58579643660793 5 12.602240959603689 38.7222399226644 34.055845870681964 14.619673247049949 6 30.00807028254352 37.12605948592718 18.064470267490144 14.801399964039156 7 27.754015348521204 34.1741384445984 21.14128270262491 16.930563504255485 8 27.074678904301187 32.8796641900291 20.439035734182784 19.606621171486935 9 27.60590788465335 16.03945171189441 20.99894251073385 35.35569789271838 10-11 24.44882696659291 26.872524374584344 27.280879701858368 21.397768956964374 12-13 26.232769331197257 24.855904918782773 27.704686524459675 21.20663922556029 14-15 23.39620226853457 25.552359853939194 26.846388984964403 24.20504889256183 16-17 21.13789716897636 28.864570628359758 27.747411322421488 22.250120880242395 18-19 21.1392740196019 26.796286405361013 31.090710534273626 20.973729040763462 20-21 22.283426477615897 25.805864933194915 30.963383781898973 20.94732480729021 22-23 21.852910042292315 25.746470205575093 31.161065318128838 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21.740724360576767 27.073731081491193 28.934920246096326 22.250624311835715 50-51 21.90955719140718 27.11403167755609 28.957917925951936 22.018493205084795 52-53 22.200689727244228 27.115978681157905 28.653067196154247 22.03026439544362 54-55 21.661797477106308 27.22308217400083 28.87056678369721 22.244553565195655 56-57 22.054637041991516 27.400915494429697 28.159061931735053 22.38538553184374 58-59 22.062473925304516 26.69283820604423 28.583969507132867 22.660718361518388 60-61 21.6058174460676 27.25412448816215 29.06005773133817 22.080000334432082 62-63 21.72059911629093 27.183752126712342 29.23806219405649 21.857586562940234 64-65 21.624860695531638 27.232905366597528 28.85857701687371 22.28365692099712 66-67 21.525957658129904 27.374028212864754 28.504766658222703 22.59524747078264 68-69 21.879054858472898 27.07880471132752 28.825779870512065 22.216360559687516 70-71 21.960135640152295 27.09914505904995 28.530498133108605 22.41022116768915 72-73 21.83710005418459 27.245691990715038 28.433822889978526 22.48338506512185 74-75 21.69567614956861 27.930335433599552 28.08569186498372 22.28829655184812 76-77 21.81196268153117 27.342543372225553 28.523065653512212 22.32242829273106 78-79 21.66946644736677 27.4270085046997 28.473978762917802 22.429546285015725 80-81 21.8951203105998 27.673047474160928 27.986255950522093 22.445576264717182 82-83 22.142493751429154 27.504371595892728 28.09998716634053 22.25314748633759 84-85 21.603830492192664 27.552898557085964 28.006419268171655 22.836851682549717 86-87 21.900754069756992 27.69144463780281 27.790306823915365 22.61749446852483 88-89 21.785950766987597 27.44752146127273 27.825831781076626 22.940695990663052 90-91 21.51010472392656 27.265050484950663 28.36429428715116 22.860550503971616 92-93 21.994128767450864 27.277276524016287 28.2932873801945 22.435307328338354 94-95 21.43906192450462 26.879911503573844 28.605274082645998 23.075752489275537 96-97 21.653960881371095 26.777761287315187 28.601625030571228 22.96665280074249 98-99 21.383612298552993 27.10908911500458 28.140825589874296 23.366472996568124 100-101 21.412184872368528 26.945871444943055 28.370967263142887 23.270976419545534 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 318.0 1 246.0 2 233.0 3 293.0 4 279.0 5 292.5 6 478.0 7 893.5 8 1475.0 9 1753.5 10 1554.0 11 1388.0 12 1413.5 13 1508.5 14 1646.0 15 1790.0 16 1858.0 17 1959.0 18 2159.5 19 2401.5 20 2674.0 21 2900.5 22 3320.5 23 3974.0 24 4546.5 25 5426.5 26 7131.0 27 8886.0 28 10206.0 29 12104.5 30 14908.5 31 18493.0 32 23927.5 33 28153.0 34 30198.5 35 33001.5 36 38295.5 37 42541.5 38 42481.0 39 41077.5 40 38973.5 41 37313.0 42 35390.5 43 35143.0 44 36719.5 45 38469.0 46 38487.5 47 38516.0 48 42558.5 49 41577.5 50 36168.5 51 33485.5 52 33021.0 53 34206.5 54 34721.5 55 32704.0 56 29827.0 57 27516.0 58 26748.5 59 24639.5 60 20740.5 61 18106.5 62 15671.0 63 11631.5 64 7757.5 65 6001.0 66 4731.0 67 3730.5 68 2936.0 69 2338.5 70 1922.0 71 1468.0 72 1152.0 73 1003.5 74 789.0 75 589.0 76 436.0 77 280.5 78 184.5 79 134.0 80 95.0 81 66.5 82 54.0 83 32.0 84 11.0 85 10.5 86 11.0 87 11.0 88 7.0 89 2.0 90 3.0 91 2.5 92 1.0 93 2.5 94 2.0 95 1.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07607868709922834 2 0.028675812829709144 3 0.01354367836271977 4 0.02064992935550484 5 0.025331694715457348 6 0.03218713684967353 7 0.08076045245918086 8 0.057853243376556056 9 0.07072809811642547 10-11 0.060612140820813794 12-13 0.040004012941737103 14-15 0.050203573190205075 16-17 0.06119736149080786 18-19 0.05722622123013384 20-21 0.018852465869094497 22-23 0.014045296079857541 24-25 0.02269820170048406 26-27 0.023576032705475158 28-29 0.005350588982802873 30-31 0.0012958457692725707 32-33 0.01032496467775242 34-35 0.011620810447024989 36-37 0.00426375059567104 38-39 0.009363530719905028 40-41 0.005183383077090283 42-43 0.0026752944914014364 44-45 3.76213287853327E-4 46-47 6.270221464222117E-4 48-49 0.0010868383871318334 50-51 0.0012540442928444234 52-53 4.180147642814744E-4 54-55 2.9261033499703213E-4 56-57 0.004598162407096218 58-59 0.003929338784245859 60-61 0.0060612140820813796 62-63 0.0029261033499703207 64-65 7.52426575706654E-4 66-67 7.52426575706654E-4 68-69 0.002842500397114026 70-71 0.002006470868551077 72-73 0.018225443722672284 74-75 0.01425430346199828 76-77 0.021820370695492964 78-79 0.01697139942982786 80-81 0.010157758772039829 82-83 0.004723566836380661 84-85 0.0036367284492488275 86-87 0.0017138605335540453 88-89 0.0018392649628384875 90-91 0.006730037704931738 92-93 0.01078478091846204 94-95 0.012498641452016084 96-97 0.01274945031058497 98-99 0.023576032705475158 100-101 0.020524524926220394 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1196130.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.20185915038887 #Duplication Level Percentage of deduplicated Percentage of total 1 74.64271365347487 32.99346715513705 2 13.03136260115724 11.520209084679948 3 4.696255643926349 6.227496915211539 4 2.2882447778217947 4.045786934835674 5 1.239401231495632 2.739191933269422 6 0.7962292464580676 2.111688780201586 7 0.5270156659238718 1.6306550564650766 8 0.3758884680741619 1.3291975297655638 9 0.2905303354034189 1.1557782867975444 >10 1.7743937683468345 15.249965985518827 >50 0.20210885620252894 6.258822625754376 >100 0.12631532056130154 10.346443350428428 >500 0.007250727677008063 2.1606466830570747 >1k 0.0019080862307915953 1.2010995945591303 >5k 3.8161724615831905E-4 1.0295500843187488 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6983 0.5837994197955072 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5224 0.43674182572128445 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3173 0.2652721694130237 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1806 0.15098693285846856 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1379 0.11528847198883063 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1243 0.10391847040037454 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1225 0.10241361724896124 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 4.1801476428147444E-5 16-17 0.0 0.0 0.0 0.0 8.360295285629489E-5 18-19 0.0 0.0 0.0 0.0 8.360295285629489E-5 20-21 0.0 0.0 0.0 0.0 8.360295285629489E-5 22-23 0.0 0.0 0.0 8.360295285629489E-5 8.360295285629489E-5 24-25 0.0 0.0 0.0 5.016177171377693E-4 8.360295285629489E-5 26-27 0.0 0.0 0.0 0.0010032354342755386 8.360295285629489E-5 28-29 0.0 0.0 0.0 0.0020482723449792247 8.360295285629489E-5 30-31 0.0 0.0 0.0 0.004263750595671039 8.360295285629489E-5 32-33 0.0 0.0 0.0 0.006395625893506559 1.6720590571258978E-4 34-35 0.0 0.0 0.0 0.008652905620626521 1.6720590571258978E-4 36-37 0.0 0.0 0.0 0.012456839975587938 1.6720590571258978E-4 38-39 0.0 0.0 0.0 0.016427980236261942 1.6720590571258978E-4 40-41 4.1801476428147444E-5 0.0 0.0 0.02207117955406185 1.6720590571258978E-4 42-43 8.360295285629489E-5 0.0 0.0 0.03126750436825429 1.6720590571258978E-4 44-45 8.360295285629489E-5 0.0 0.0 0.04585621964167774 1.6720590571258978E-4 46-47 8.360295285629489E-5 0.0 0.0 0.06429067074649077 1.6720590571258978E-4 48-49 8.360295285629489E-5 0.0 0.0 0.09844247698828723 1.6720590571258978E-4 50-51 8.360295285629489E-5 0.0 0.0 0.1320926655129459 1.6720590571258978E-4 52-53 8.360295285629489E-5 0.0 0.0 0.16402899350405056 1.6720590571258978E-4 54-55 8.360295285629489E-5 0.0 0.0 0.2273164288162658 1.6720590571258978E-4 56-57 8.360295285629489E-5 0.0 0.0 0.2868835327263759 1.6720590571258978E-4 58-59 8.360295285629489E-5 0.0 0.0 0.3655121098877212 1.6720590571258978E-4 60-61 8.360295285629489E-5 0.0 0.0 0.45450745320324715 1.6720590571258978E-4 62-63 8.360295285629489E-5 0.0 0.0 0.5676222484178142 1.6720590571258978E-4 64-65 8.360295285629489E-5 0.0 0.0 0.655196341534783 1.6720590571258978E-4 66-67 8.360295285629489E-5 0.0 0.0 0.7839448889334771 1.6720590571258978E-4 68-69 8.360295285629489E-5 0.0 0.0 0.9502311621646476 1.6720590571258978E-4 70-71 8.360295285629489E-5 0.0 0.0 1.057201140344277 1.6720590571258978E-4 72-73 8.360295285629489E-5 0.0 0.0 1.220185096937624 1.6720590571258978E-4 74-75 8.360295285629489E-5 0.0 0.0 1.4166102346734886 1.6720590571258978E-4 76-77 8.360295285629489E-5 0.0 0.0 1.5648800715641276 1.6720590571258978E-4 78-79 8.360295285629489E-5 0.0 0.0 1.7020725172013074 1.6720590571258978E-4 80-81 8.360295285629489E-5 0.0 0.0 1.9177263340941202 1.6720590571258978E-4 82-83 8.360295285629489E-5 0.0 0.0 2.1933652696613244 1.6720590571258978E-4 84-85 8.360295285629489E-5 0.0 0.0 2.5255198013593843 1.6720590571258978E-4 86-87 8.360295285629489E-5 0.0 0.0 2.9654385392892078 1.6720590571258978E-4 88-89 8.360295285629489E-5 0.0 0.0 3.4407213262772443 1.6720590571258978E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9850 0.0 33.20427 1 GGTATCA 6375 0.0 32.064903 1 CGTATAG 120 5.348469E-6 27.730553 1 ATCAACG 11920 0.0 27.279709 3 GTACATG 8415 0.0 27.172691 1 TCAACGC 12030 0.0 27.030268 4 TACATGG 8370 0.0 26.807621 2 TATCAAC 12195 0.0 26.780375 2 CAACGCA 12155 0.0 26.714298 5 AACGCAG 12405 0.0 26.175924 6 ACATGGG 8435 0.0 25.700384 3 GAGTACT 6435 0.0 23.67571 12-13 CATGGGA 5195 0.0 23.609894 4 ACGCAGA 13755 0.0 23.53773 7 CGCAGAG 13850 0.0 23.378237 8 GTACTTT 6715 0.0 22.682318 14-15 GCAGAGT 14380 0.0 22.08491 9 GTATAGG 520 0.0 21.940655 1 ATGGGAT 2115 0.0 21.804163 5 AGTACTT 6905 0.0 21.134804 12-13 >>END_MODULE