FastQCFastQC Report
Thu 26 May 2016
SRR936652_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936652_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196130
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72500.6061214082081379No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64160.536396545525988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38620.32287460393101086No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25530.21343833864212086No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20310.1697975972511349No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17840.14914766789563008No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA16420.1372760485900362No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15520.12975178283296968No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA13790.11528847198883063No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA12040.10065795523897904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAG6100.043.607862
GTAAGGT7450.039.5247734
GGTAAGG7350.038.7750473
TAAGGTG8200.037.6472665
TAAGGTA2850.033.3287544
AAGGTGT10500.030.3040966
GTATCAA111900.029.974631
GGTATCA76550.029.1076341
GGTGTGC10850.028.8888328
AGGTGTG10850.028.4511247
TATAGGG4700.028.2987172
GTATAGG6750.027.4498581
GTGTGCA11650.026.49749
GTACATG84350.025.345841
GTATAGT3450.024.7874951
ATCAACG136500.024.4282823
TACATGG86850.024.2839722
TCAACGC137400.024.2652264
ACATGGG82950.023.9930843
CAACGCA139500.023.9339895