##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936652_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1196130 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.874260322874605 31.0 31.0 34.0 30.0 34.0 2 32.026607475776046 31.0 31.0 34.0 30.0 34.0 3 32.09250081512879 33.0 31.0 34.0 30.0 34.0 4 35.819262956367616 37.0 35.0 37.0 35.0 37.0 5 35.6537332898598 37.0 35.0 37.0 33.0 37.0 6 35.755363547440496 37.0 35.0 37.0 33.0 37.0 7 35.695424410390174 37.0 35.0 37.0 33.0 37.0 8 35.665742018008075 37.0 35.0 37.0 33.0 37.0 9 37.28624480616655 39.0 37.0 39.0 34.0 39.0 10-11 37.17668230041885 39.0 37.0 39.0 33.0 39.0 12-13 37.07650631620309 39.0 37.0 39.0 33.0 39.0 14-15 38.16831155476412 40.0 37.0 41.0 33.0 41.0 16-17 38.0814811099128 40.0 37.0 41.0 33.0 41.0 18-19 38.2659547875231 40.0 38.0 41.0 33.5 41.0 20-21 38.292903363346795 40.0 38.0 41.0 34.0 41.0 22-23 38.20549313201742 40.0 37.5 41.0 33.0 41.0 24-25 38.05548602576643 40.0 37.0 41.0 33.0 41.0 26-27 37.83394530694824 40.0 37.0 41.0 33.0 41.0 28-29 37.73991037763454 40.0 37.0 41.0 32.5 41.0 30-31 37.64862431341075 39.5 37.0 41.0 33.0 41.0 32-33 37.43265364132661 39.0 36.0 41.0 32.0 41.0 34-35 37.243188031401274 39.0 36.0 41.0 32.0 41.0 36-37 36.99896959360605 39.0 35.0 41.0 31.0 41.0 38-39 36.77946836882279 39.0 35.0 41.0 31.0 41.0 40-41 36.54610326636737 39.0 35.0 41.0 30.5 41.0 42-43 36.21298228453429 39.0 35.0 40.0 30.0 41.0 44-45 35.83411878307542 39.0 35.0 40.0 29.5 41.0 46-47 35.33083402305769 38.0 35.0 40.0 27.5 41.0 48-49 34.843890714220024 38.0 34.0 40.0 25.0 41.0 50-51 34.508869019253765 38.0 34.0 40.0 24.0 41.0 52-53 34.139800857766296 38.0 33.0 40.0 23.0 41.0 54-55 33.93998980043975 38.0 33.0 40.0 23.0 41.0 56-57 33.62894585036743 37.5 33.0 40.0 22.0 41.0 58-59 33.245272253015976 37.0 32.5 40.0 20.5 41.0 60-61 32.82117035773703 36.0 32.0 39.0 18.5 41.0 62-63 32.49157825654402 36.0 31.5 39.0 18.0 40.0 64-65 32.06146572696947 35.0 31.0 39.0 16.0 40.0 66-67 31.648525661926378 35.0 31.0 38.0 13.0 40.0 68-69 31.278551244429956 35.0 31.0 38.0 9.5 40.0 70-71 30.910607542658408 35.0 30.0 37.0 7.5 39.5 72-73 30.472489612333106 34.0 30.0 37.0 7.0 39.0 74-75 29.976289784555192 34.0 29.0 36.0 3.5 39.0 76-77 28.497690886442108 32.5 27.0 34.5 2.0 37.0 78-79 29.257250048071697 34.0 29.0 35.0 2.0 37.0 80-81 29.03657043966793 34.0 29.0 35.0 2.0 37.0 82-83 28.75719570615234 34.0 29.0 35.0 2.0 36.5 84-85 28.393626946903765 33.5 28.5 35.0 2.0 36.0 86-87 28.041115514199962 33.0 27.0 35.0 2.0 36.0 88-89 27.57429794420339 33.0 26.5 35.0 2.0 35.0 90-91 27.11252330432311 33.0 26.0 35.0 2.0 35.0 92-93 26.50661591967428 32.5 25.0 34.5 2.0 35.0 94-95 25.98610602526481 32.0 24.0 34.0 2.0 35.0 96-97 25.299650121642298 32.0 21.5 34.0 2.0 35.0 98-99 24.469877856085876 32.0 10.0 34.0 2.0 35.0 100-101 21.34613670754851 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30972141792130614 1101 2 0.33076602749662953 1101 3 0.31740981052231376 1101 4 0.2518284659115082 1101 5 0.20208436319796164 1101 6 0.2715121791186519 1101 7 0.2999509633465891 1101 8 0.269241865937893 1101 9 0.2787276661557172 1101 10-11 0.38552333714738296 1101 12-13 0.34993247027064456 1101 14-15 0.5960945321994657 1101 16-17 0.41672773621256454 1101 18-19 0.36856491049789497 1101 20-21 0.3190339646483409 1101 22-23 0.27486587348302294 1101 24-25 0.2638281718208333 1101 26-27 0.25906261954355614 1101 28-29 0.2536911420319896 1101 30-31 0.39548093470572354 1101 32-33 0.4537776176146764 1101 34-35 0.4391007878245503 1101 36-37 0.49716947783213783 1101 38-39 0.6394160924001611 1101 40-41 0.753735325577999 1101 42-43 0.7631061074788121 1101 44-45 0.7058328101860099 1101 46-47 0.7879923410604732 1101 48-49 0.6629737888579541 1101 50-51 0.659731845502904 1101 52-53 0.8238235407202836 1101 54-55 0.6008159704878864 1101 56-57 0.6450409692520651 1101 58-59 0.6290972929530838 1101 60-61 0.7904998428967573 1101 62-63 0.8271820727251864 1101 64-65 0.7665615531765368 1101 66-67 0.6256226216866274 1101 68-69 0.6243766900164971 1101 70-71 0.6027848121242734 1101 72-73 0.6810876807164021 1101 74-75 0.703910819652922 1101 76-77 0.4903570203417118 1101 78-79 0.7069605038574132 1101 80-81 0.7551636197935672 1101 82-83 0.7449736988992264 1101 84-85 0.7015800748321546 1101 86-87 0.7834936722038925 1101 88-89 0.7063244094307564 1101 90-91 0.7867459923971616 1101 92-93 0.8705166608016235 1101 94-95 0.7317114016019879 1101 96-97 0.9837076731209855 1101 98-99 0.7992606079483373 1101 100-101 1.331784825188759 1102 1 0.24493867510445355 1102 2 0.2931051778579885 1102 3 0.25931400583343844 1102 4 0.21596560675409648 1102 5 0.2159315340070549 1102 6 0.20002046397674889 1102 7 0.19568514279308857 1102 8 0.3106895126856415 1102 9 0.19059969153911283 1102 10-11 0.31005356806576145 1102 12-13 0.26446865042223067 1102 14-15 0.44532113188217437 1102 16-17 0.47310950071106816 1102 18-19 0.3687339127894518 1102 20-21 0.3235939417327742 1102 22-23 0.37898252252233533 1102 24-25 0.3025228659861767 1102 26-27 0.3591710277931597 1102 28-29 0.41296489489643307 1102 30-31 0.4788347598423428 1102 32-33 0.4422991229910238 1102 34-35 0.4152509729118137 1102 36-37 0.3190353336403504 1102 38-39 0.2577218829871555 1102 40-41 0.41676216323003956 1102 42-43 0.3471703151290839 1102 44-45 0.12894029299933862 1102 46-47 0.0909345673957489 1102 48-49 0.1296715237424948 1102 50-51 0.2440135306782949 1102 52-53 0.03958041538977142 1102 54-55 0.10015736833734934 1102 56-57 -0.016620188865331897 1102 58-59 0.034891625757587974 1102 60-61 0.24543748611903737 1102 62-63 0.3229131673840939 1102 64-65 0.10778665453383951 1102 66-67 -0.04265121931460314 1102 68-69 0.24555992639935909 1102 70-71 0.126644101741757 1102 72-73 0.25117317340104606 1102 74-75 0.11727213641653833 1102 76-77 0.01716889236709207 1102 78-79 0.16351347406717665 1102 80-81 0.18328850962258159 1102 82-83 0.15740363015252967 1102 84-85 0.17910166834528596 1102 86-87 0.16779188302708903 1102 88-89 0.07276566626135761 1102 90-91 0.07459611578866188 1102 92-93 0.16507090899481724 1102 94-95 0.09166975696951951 1102 96-97 0.3010501286119087 1102 98-99 0.3256738813486564 1102 100-101 0.7945529813671435 1103 1 0.11901466150779427 1103 2 0.24736919403629543 1103 3 0.11124077318424241 1103 4 0.11229752018294903 1103 5 0.2355171800635958 1103 6 0.13566953243336144 1103 7 0.10307940293679962 1103 8 0.016696569180631116 1103 9 0.12986220175812235 1103 10-11 0.16501914613899515 1103 12-13 0.29354700333303896 1103 14-15 0.34464990431020937 1103 16-17 0.21295847450737426 1103 18-19 0.2526586578520593 1103 20-21 0.029687698455809652 1103 22-23 0.18840251305630318 1103 24-25 0.24795314007076996 1103 26-27 0.13651795778756082 1103 28-29 0.1164567842299391 1103 30-31 0.15606164336714556 1103 32-33 0.06620988414587714 1103 34-35 0.018020862331368903 1103 36-37 -0.0698760729256307 1103 38-39 -0.06149566359001568 1103 40-41 -0.04752250610433606 1103 42-43 0.10866056942107605 1103 44-45 0.1056005055547331 1103 46-47 0.21621338500256115 1103 48-49 0.12768623911618704 1103 50-51 0.2129978687016134 1103 52-53 0.15124342472671515 1103 54-55 0.16120723710375273 1103 56-57 0.12813670627687657 1103 58-59 0.259153490565474 1103 60-61 0.24513693967095662 1103 62-63 0.3349343798964597 1103 64-65 0.5111217089635076 1103 66-67 0.19856839257817072 1103 68-69 0.1983278795855412 1103 70-71 0.2877421612056388 1103 72-73 0.18078076343590155 1103 74-75 0.2059243028465545 1103 76-77 0.23241156695080534 1103 78-79 -0.06422737185167549 1103 80-81 0.24087079954125912 1103 82-83 0.25185287932425027 1103 84-85 0.42390460281454523 1103 86-87 0.3211518933536688 1103 88-89 0.39538280838088724 1103 90-91 0.33326248126347124 1103 92-93 0.34893455513741145 1103 94-95 0.23576747221413186 1103 96-97 0.1738296991983752 1103 98-99 0.19299843709066167 1103 100-101 0.3773170620865649 1104 1 0.2398020630825961 1104 2 0.22074452212028461 1104 3 0.1489205632104884 1104 4 0.1867962078469887 1104 5 0.20274027717645282 1104 6 0.053506802774563766 1104 7 0.16949935044335973 1104 8 0.12460208099165726 1104 9 0.34386745110195704 1104 10-11 0.1581792511258726 1104 12-13 0.21039214768999415 1104 14-15 0.28893861822096767 1104 16-17 0.3762734351372998 1104 18-19 0.07208910404627744 1104 20-21 0.15804202916446997 1104 22-23 0.16491694875183782 1104 24-25 0.10778516106814351 1104 26-27 0.2347830496510852 1104 28-29 0.25232555063414 1104 30-31 0.30906426803906584 1104 32-33 0.19463245632434933 1104 34-35 0.2479394975019673 1104 36-37 0.3103522735310662 1104 38-39 0.3545961999270446 1104 40-41 0.2532648954704726 1104 42-43 0.17268944081214954 1104 44-45 0.3491096919064418 1104 46-47 0.4043498679421873 1104 48-49 0.5095786275676275 1104 50-51 0.5242157164706427 1104 52-53 0.5354875192149038 1104 54-55 0.4558240350040137 1104 56-57 0.37087161441336036 1104 58-59 0.2731429918778119 1104 60-61 0.39313693967095276 1104 62-63 0.3775852985316348 1104 64-65 0.40983037038083836 1104 66-67 0.24432692276190338 1104 68-69 0.2441992706616567 1104 70-71 0.29953481212427135 1104 72-73 0.5012059602862955 1104 74-75 0.4530371637389443 1104 76-77 0.26766616012665523 1104 78-79 0.39189598772837897 1104 80-81 0.5388786735570044 1104 82-83 0.5630812257809445 1104 84-85 0.583096203864411 1104 86-87 0.5368028119888386 1104 88-89 0.4889077427640913 1104 90-91 0.6967217988487739 1104 92-93 0.8012075210166749 1104 94-95 0.7037517241826343 1104 96-97 0.9469845548414177 1104 98-99 0.9010509305289816 1104 100-101 1.3750939649737042 1105 1 0.0569686176667723 1105 2 0.11982858398982188 1105 3 0.12197907027168498 1105 4 0.11063525738745028 1105 5 0.13068871922980918 1105 6 0.07581659878442792 1105 7 0.17619381356821862 1105 8 0.2093315138542451 1105 9 0.09683405051912786 1105 10-11 0.17707882521239782 1105 12-13 0.07145289640104835 1105 14-15 0.2088906469140852 1105 16-17 0.10198224482122953 1105 18-19 -0.031837818168462206 1105 20-21 0.06589497693402535 1105 22-23 0.1087273051741704 1105 24-25 0.08716175824366701 1105 26-27 -0.09529070062912126 1105 28-29 0.12387430651847353 1105 30-31 0.12783494725896105 1105 32-33 0.12684518889552265 1105 34-35 0.33933290705140706 1105 36-37 0.10071833495226912 1105 38-39 0.3421021402991613 1105 40-41 0.4546547183801337 1105 42-43 0.29600932424635573 1105 44-45 0.3090655314043076 1105 46-47 0.26789952048646626 1105 48-49 0.26217199228405974 1105 50-51 0.2926490273874762 1105 52-53 0.36784416570672107 1105 54-55 0.245093481316502 1105 56-57 0.3462993212055636 1105 58-59 0.10984328329229243 1105 60-61 0.22196388430219827 1105 62-63 0.1313680826607495 1105 64-65 0.4012901327659506 1105 66-67 0.10227827896006403 1105 68-69 0.2826801045554035 1105 70-71 0.42725662337735315 1105 72-73 0.493899975081181 1105 74-75 0.37653525833656687 1105 76-77 0.23783719801054204 1105 78-79 0.3205895541932975 1105 80-81 0.26580738909164126 1105 82-83 0.4645622838396726 1105 84-85 0.4588780913335917 1105 86-87 0.6095436772656839 1105 88-89 0.5357138400516952 1105 90-91 0.4470820319803579 1105 92-93 0.296484140608694 1105 94-95 0.6659716300333436 1105 96-97 0.8504183140882233 1105 98-99 0.6360300831853394 1105 100-101 1.087447024825753 1106 1 0.01606584881296058 1106 2 0.06091602785964767 1106 3 0.06674230527666225 1106 4 0.10484662423231583 1106 5 0.13985799043326352 1106 6 -0.018829006363404233 1106 7 -0.013477917168614795 1106 8 0.029804421752402277 1106 9 0.015168824048160445 1106 10-11 -0.06531996111809946 1106 12-13 0.05473065770574692 1106 14-15 0.10392353832000367 1106 16-17 0.1340229287227146 1106 18-19 0.09389103967536272 1106 20-21 -0.05971264110336705 1106 22-23 -0.07343686623803336 1106 24-25 -0.07237756681841745 1106 26-27 -0.26150706963768755 1106 28-29 -0.37680409149955096 1106 30-31 -0.14228211953690106 1106 32-33 -0.1623868999664424 1106 34-35 0.06436488575315735 1106 36-37 -0.20854284238160403 1106 38-39 0.13722775518250074 1106 40-41 0.2813315171225099 1106 42-43 0.19726517792671672 1106 44-45 0.06407007903225548 1106 46-47 0.00700235500048052 1106 48-49 0.00838506465968436 1106 50-51 0.2995258672696579 1106 52-53 -0.1348458141184281 1106 54-55 0.04057600439406883 1106 56-57 -0.19905208578920508 1106 58-59 -0.2152072219416823 1106 60-61 -0.19929812672562264 1106 62-63 -0.19255312145485703 1106 64-65 -0.17713204240331848 1106 66-67 -0.017855611561753193 1106 68-69 -0.2319499528359792 1106 70-71 0.07640201896649401 1106 72-73 0.1423052175449051 1106 74-75 0.11737995014452451 1106 76-77 -0.18484813201829198 1106 78-79 -0.49205044486221183 1106 80-81 -0.2247209213113237 1106 82-83 -0.1816315808812199 1106 84-85 0.10618173166094991 1106 86-87 0.2857586976516764 1106 88-89 0.1088325683323994 1106 90-91 0.010876874248278767 1106 92-93 0.006840426711026026 1106 94-95 -0.20366015967961815 1106 96-97 0.1573005575422961 1106 98-99 0.17632574484363417 1106 100-101 0.579379582799497 1107 1 0.04946085573351411 1107 2 0.08267103130663855 1107 3 -0.07839177274751563 1107 4 0.05783557792572935 1107 5 -0.03876103515950291 1107 6 -0.032309470191314915 1107 7 -0.08917781491096832 1107 8 -0.027266685412541847 1107 9 -0.13982283761195902 1107 10-11 -0.03609633942530621 1107 12-13 -0.06832963708690443 1107 14-15 -0.014602064193724118 1107 16-17 -0.01998115803855427 1107 18-19 -0.047538192541644264 1107 20-21 0.030015782445303785 1107 22-23 -0.08292294626128438 1107 24-25 -0.009920875677259744 1107 26-27 0.06762031999228668 1107 28-29 -0.064973661964288 1107 30-31 -0.19761289731527398 1107 32-33 -0.19494497149717205 1107 34-35 -0.25330459698621866 1107 36-37 -0.22079470809623558 1107 38-39 -0.24850353760576382 1107 40-41 -0.2903046583353017 1107 42-43 -0.41036830197000285 1107 44-45 -0.15457009811981237 1107 46-47 -0.27525643127040667 1107 48-49 -0.28537150366596364 1107 50-51 -0.30242207880494476 1107 52-53 -0.06693242829165769 1107 54-55 -0.21280851092774355 1107 56-57 -0.42304439608533073 1107 58-59 -0.3058071393557853 1107 60-61 -0.17710715481723582 1107 62-63 -0.10896325789881445 1107 64-65 -0.22641109943543825 1107 66-67 -0.10851822159505176 1107 68-69 0.01394467748580297 1107 70-71 -0.17157542409620063 1107 72-73 -0.041988265435488614 1107 74-75 -0.010611130224312149 1107 76-77 -0.16063305247176984 1107 78-79 0.11031331056302562 1107 80-81 0.18542460531553573 1107 82-83 -0.05654607080698426 1107 84-85 0.07121431410063295 1107 86-87 0.05934086973162067 1107 88-89 0.03580275588744897 1107 90-91 0.1905459458303973 1107 92-93 0.2908636889956817 1107 94-95 0.48478322024562814 1107 96-97 0.5993546335815729 1107 98-99 0.34424515625076424 1107 100-101 0.947527035839844 1108 1 -0.022626474597537083 1108 2 0.06632496152496259 1108 3 -0.014003604046777696 1108 4 0.07430813792022661 1108 5 -0.0174265076097484 1108 6 0.01499321902781503 1108 7 0.012162237284840671 1108 8 0.03984753338948366 1108 9 0.07062199870412655 1108 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-1.6035566780891841 2316 60-61 -1.0084109151145277 2316 62-63 -0.7231572757257894 2316 64-65 -0.6995887715333566 2316 66-67 -0.8648050904394182 2316 68-69 -1.2718106303284422 2316 70-71 -1.0813529766546068 2316 72-73 -0.42326928723845825 2316 74-75 -0.6163522752049531 2316 76-77 -0.7106869751868743 2316 78-79 -0.8935957614465373 2316 80-81 -0.7676658808984378 2316 82-83 -0.9287966620078336 2316 84-85 -1.0372041261685894 2316 86-87 -1.0429628645788185 2316 88-89 -1.1358579338035675 2316 90-91 -1.3294795212832398 2316 92-93 -1.5936208618216057 2316 94-95 -1.8617631273025133 2316 96-97 -2.013530296643733 2316 98-99 -2.599721346698743 2316 100-101 -3.5007773221550096 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 23.0 5 36.0 6 106.0 7 265.0 8 623.0 9 1138.0 10 1924.0 11 3011.0 12 4391.0 13 5987.0 14 7593.0 15 10641.0 16 13907.0 17 17836.0 18 14961.0 19 9374.0 20 7986.0 21 8141.0 22 8799.0 23 9702.0 24 11126.0 25 13254.0 26 15860.0 27 19588.0 28 24251.0 29 29384.0 30 36463.0 31 44723.0 32 55413.0 33 70948.0 34 92875.0 35 126502.0 36 175289.0 37 218910.0 38 128145.0 39 6948.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.23545988035769 20.445054678576266 15.154218838061439 21.165266603004603 2 15.750302890440201 21.928013665814643 39.09038384517428 23.231299598570875 3 18.988729285463453 26.881573886724304 30.196485008611894 23.93321181920035 4 12.601138672217902 17.74288747878575 37.49617516490682 32.15979868408952 5 12.618260924817989 39.383187134013994 33.589948532042904 14.408603409125112 6 30.345865415966493 37.834516315116254 16.952839574293765 14.866778694623495 7 27.917701253208264 34.69731550918379 20.528788676816063 16.856194560791888 8 27.625508932975514 33.36660730857014 19.480909265715265 19.526974492739082 9 28.005233544848807 16.232600135436783 20.253651358966 35.508514960748414 10-11 24.83735045521808 27.376999155610175 26.46806785215654 21.317582537015205 12-13 26.517930072175094 25.165639873624613 27.184920563923825 21.13150949027647 14-15 23.40500063120282 26.22493058859036 26.433143916751455 23.936924863455364 16-17 21.279501391989164 29.314622992484097 27.333943634889184 22.071931980637558 18-19 21.187696156052017 27.02066859847807 31.140301008097893 20.651334237372023 20-21 22.285203113373964 25.91754240759784 31.160408985645372 20.63684549338283 22-23 21.875348781214743 25.87989009551223 31.265719974300442 20.979041148972584 24-25 21.72573215285964 26.2829709145327 31.294967938267582 20.696328994340078 26-27 21.168126396190324 26.408689152855253 31.287789103361558 21.135395347592866 28-29 20.979703560707684 26.483523695621546 31.512549363701005 21.024223379969758 30-31 21.18564785630801 26.411654741939312 31.017946449920323 21.384750951832352 32-33 21.044941838056214 26.496646898935417 30.946393023074986 21.51201823993338 34-35 21.760596733665714 26.232337035468685 31.003046120700283 21.004020110165314 36-37 22.288436176060564 25.7338190695351 30.764700103877317 21.213044650527017 38-39 21.48763750733623 26.44989875580837 30.76251674165927 21.299946995196127 40-41 21.443028767776077 26.47822561092858 31.05891500087783 21.019830620417512 42-43 21.523867172036184 26.63916591456234 30.05070276990373 21.786264143497743 44-45 21.56198831802755 26.574000107849027 30.110653937137393 21.753357636986028 46-47 22.43744918497401 27.09272213873521 29.05543096367235 21.41439771261843 48-49 22.257103348643874 27.386872286030066 28.403487592444275 21.952536772881786 50-51 22.296015854483862 27.447845277493176 28.47449082602747 21.78164804199549 52-53 22.533908355164183 27.461639782013386 28.37654984393399 21.627902018888438 54-55 21.9850231416456 27.661414650133096 28.41748933210268 21.936072876118622 56-57 22.369981659323287 27.909016530857027 27.68672936862001 22.034272441199672 58-59 22.30154764717073 27.13406182644526 28.03116618130656 22.533224345077446 60-61 21.976518221331776 27.716935946470713 28.426439870692178 21.880105961505336 62-63 21.890101326648736 27.7321133610957 28.81427712004388 21.563508192211682 64-65 21.782872814994125 27.71124684869997 28.47671487162764 22.029165464678265 66-67 21.827022638908222 27.79765480357276 28.038143824389927 22.337178733129086 68-69 22.23196665564496 27.487983065308004 28.168639635756982 22.111410643290057 70-71 22.27544019081554 27.682925503854516 27.893479716652635 22.148154588677308 72-73 22.045262638676398 27.6887545668113 27.899308603579883 22.36667419093242 74-75 22.048283225023805 28.427007906470063 27.350350177115967 22.174358691390168 76-77 22.108324418956084 27.829996032945363 27.969240068673102 22.092439479425448 78-79 22.073327346881445 27.802972156309934 27.733367111637296 22.390333385171324 80-81 22.376574565309184 28.258143005857196 27.10936356833185 22.25591886050177 82-83 22.54127163502442 28.24402555795672 27.169940846115033 22.044761960903834 84-85 21.98962117844908 28.325293391330746 27.16887139619025 22.516214034029925 86-87 22.054168985126875 28.41628857126971 26.894528628994667 22.635013814608744 88-89 21.918538011426907 28.21630084474484 26.93921023612795 22.925950907700305 90-91 21.723897106485836 28.1275588509583 27.33561669614965 22.812927346406212 92-93 22.13960917459196 28.003704625136677 27.289999811841838 22.566686388429527 94-95 21.692857761095237 27.64191506149259 27.59303542540127 23.0721917520109 96-97 22.14761844818667 27.418678285060206 27.45276228895624 22.980940977796884 98-99 21.581110882019967 27.7196894639309 27.09110611013968 23.608093543909455 100-101 21.279899888621074 27.944734637508223 27.161652523675865 23.61371295019484 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 563.0 1 406.5 2 283.5 3 336.0 4 313.0 5 306.0 6 498.0 7 887.5 8 1353.0 9 1651.0 10 1564.5 11 1406.0 12 1459.5 13 1585.0 14 1716.5 15 1876.5 16 2019.0 17 2201.0 18 2427.0 19 2637.0 20 2901.0 21 3246.0 22 3606.0 23 4143.5 24 4790.5 25 5713.5 26 7144.0 27 8775.5 28 10205.5 29 12333.0 30 15532.0 31 18718.5 32 23652.5 33 27056.5 34 29215.5 35 32791.0 36 37922.0 37 41775.0 38 42016.5 39 41516.5 40 39444.0 41 36831.5 42 34167.5 43 34726.5 44 36518.5 45 37756.5 46 37493.0 47 37283.0 48 42068.0 49 41050.5 50 35614.5 51 33003.5 52 32215.0 53 34200.0 54 35874.5 55 34265.0 56 30346.5 57 27743.5 58 27630.0 59 25600.5 60 21298.0 61 18250.5 62 15798.0 63 11681.5 64 7783.5 65 6044.5 66 4757.0 67 3877.0 68 3087.0 69 2484.0 70 2040.0 71 1487.0 72 1189.5 73 1117.0 74 867.0 75 633.5 76 499.5 77 323.0 78 231.0 79 179.5 80 124.0 81 93.0 82 76.0 83 52.5 84 31.0 85 15.5 86 8.0 87 3.0 88 2.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019395885062660413 2 0.012791251787013118 3 0.009196324814192436 4 0.0 5 0.0038457358313895647 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.196324814192438E-4 14-15 8.360295285629489E-5 16-17 0.0 18-19 0.0013376472457007182 20-21 0.0 22-23 4.1801476428147444E-5 24-25 0.0 26-27 0.001504853151413308 28-29 0.0028006989206858786 30-31 0.0013376472457007182 32-33 0.006103015558509527 34-35 0.0015466546278414554 36-37 6.270221464222117E-4 38-39 0.0010032354342755386 40-41 0.0 42-43 0.0034695225435362378 44-45 0.001128639863559981 46-47 2.090073821407372E-4 48-49 3.3441181142517955E-4 50-51 1.2540442928444232E-4 52-53 1.2540442928444232E-4 54-55 0.001504853151413308 56-57 0.01291665621629756 58-59 0.023617834181903306 60-61 0.018727061439810056 62-63 0.012247832593447201 64-65 0.016302575806977502 66-67 0.00213187529783552 68-69 4.598162407096219E-4 70-71 8.360295285629489E-5 72-73 0.0 74-75 0.0017556620099821927 76-77 0.002382684156404404 78-79 0.008025883474204309 80-81 0.013794487221288655 82-83 0.01517393594341752 84-85 0.027547172966149166 86-87 0.009488935149189468 88-89 0.03707790959176678 90-91 0.043891550249554816 92-93 0.027379967060436574 94-95 0.02804879068328693 96-97 0.04635783735881551 98-99 0.04886592594450436 100-101 0.05530335331443907 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1196130.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.34876437777279 #Duplication Level Percentage of deduplicated Percentage of total 1 72.69454947806368 30.058297979168003 2 13.679715690291117 11.312786816655375 3 5.155613494923469 6.395347428733687 4 2.4764125164170836 4.095863905739895 5 1.3964770160745668 2.8871299548321248 6 0.8812307722175509 2.186268213771976 7 0.604531965269779 1.749765485354041 8 0.43909142420000963 1.4524710271637504 9 0.3303634735344508 1.2294109283578725 >10 1.954485943062442 15.559707694123423 >50 0.23285567280292982 6.719709285464938 >100 0.142355287805281 10.81276843208779 >500 0.008884584833764472 2.422365297169926 >1k 0.0030288357387833423 1.9771002735664651 >5k 4.0384476517111233E-4 1.1410072778107816 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7250 0.6061214082081379 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6416 0.536396545525988 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3862 0.32287460393101086 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2553 0.21343833864212086 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2031 0.1697975972511349 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1784 0.14914766789563008 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1642 0.1372760485900362 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1552 0.12975178283296968 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1379 0.11528847198883063 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 1204 0.10065795523897904 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 8.360295285629489E-5 0.0 24-25 0.0 0.0 0.0 4.180147642814744E-4 0.0 26-27 0.0 0.0 0.0 9.196324814192438E-4 4.1801476428147444E-5 28-29 0.0 0.0 0.0 0.00196466939212293 8.360295285629489E-5 30-31 0.0 0.0 0.0 0.0041801476428147445 8.360295285629489E-5 32-33 0.0 0.0 0.0 0.006646434752075443 8.360295285629489E-5 34-35 0.0 0.0 0.0 0.009238126290620586 8.360295285629489E-5 36-37 0.0 0.0 0.0 0.01308386212201015 8.360295285629489E-5 38-39 0.0 0.0 0.0 0.016762392047687126 8.360295285629489E-5 40-41 0.0 0.0 0.0 0.02274000317691221 8.360295285629489E-5 42-43 0.0 0.0 0.0 0.03218713684967353 8.360295285629489E-5 44-45 0.0 0.0 0.0 0.046775852123096984 8.360295285629489E-5 46-47 0.0 0.0 0.0 0.06521030322791001 8.360295285629489E-5 48-49 0.0 0.0 0.0 0.09936210946970647 8.360295285629489E-5 50-51 0.0 0.0 0.0 0.13317950390007774 8.360295285629489E-5 52-53 0.0 0.0 0.0 0.16507403041475427 8.360295285629489E-5 54-55 0.0 0.0 0.0 0.22719102438698136 8.360295285629489E-5 56-57 0.0 0.0 0.0 0.28659092239137884 8.360295285629489E-5 58-59 0.0 0.0 0.0 0.36526130102915233 8.360295285629489E-5 60-61 0.0 0.0 0.0 0.45388043105682496 8.360295285629489E-5 62-63 0.0 0.0 0.0 0.5673714395592453 8.360295285629489E-5 64-65 0.0 0.0 0.0 0.6556561577754927 8.360295285629489E-5 66-67 0.0 0.0 0.0 0.784195697792046 8.360295285629489E-5 68-69 0.0 0.0 0.0 0.9499803533060788 8.360295285629489E-5 70-71 0.0 0.0 0.0 1.0568667285328517 8.360295285629489E-5 72-73 0.0 0.0 0.0 1.218513037880498 8.360295285629489E-5 74-75 0.0 0.0 0.0 1.4141439475642281 8.360295285629489E-5 76-77 0.0 0.0 0.0 1.5619121667377294 8.360295285629489E-5 78-79 0.0 0.0 0.0 1.6990210094220528 8.360295285629489E-5 80-81 0.0 0.0 0.0 1.9127519583991708 8.360295285629489E-5 82-83 0.0 0.0 0.0 2.188098283631378 8.360295285629489E-5 84-85 0.0 0.0 0.0 2.51816274150803 8.360295285629489E-5 86-87 0.0 0.0 0.0 2.953692324412898 8.360295285629489E-5 88-89 0.0 0.0 0.0 3.4249203681874043 8.360295285629489E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTAAG 610 0.0 43.60786 2 GTAAGGT 745 0.0 39.524773 4 GGTAAGG 735 0.0 38.775047 3 TAAGGTG 820 0.0 37.647266 5 TAAGGTA 285 0.0 33.328754 4 AAGGTGT 1050 0.0 30.304096 6 GTATCAA 11190 0.0 29.97463 1 GGTATCA 7655 0.0 29.107634 1 GGTGTGC 1085 0.0 28.888832 8 AGGTGTG 1085 0.0 28.451124 7 TATAGGG 470 0.0 28.298717 2 GTATAGG 675 0.0 27.449858 1 GTGTGCA 1165 0.0 26.4974 9 GTACATG 8435 0.0 25.34584 1 GTATAGT 345 0.0 24.787495 1 ATCAACG 13650 0.0 24.428282 3 TACATGG 8685 0.0 24.283972 2 TCAACGC 13740 0.0 24.265226 4 ACATGGG 8295 0.0 23.993084 3 CAACGCA 13950 0.0 23.933989 5 >>END_MODULE