##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936651_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 623728 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.757573814226713 31.0 31.0 34.0 30.0 34.0 2 31.748728612472103 33.0 31.0 34.0 30.0 34.0 3 31.789655106071876 33.0 31.0 34.0 30.0 34.0 4 35.325398250519456 37.0 35.0 37.0 33.0 37.0 5 35.389605725572686 37.0 35.0 37.0 33.0 37.0 6 35.48729734756176 37.0 35.0 37.0 33.0 37.0 7 35.45207205705051 37.0 35.0 37.0 33.0 37.0 8 35.441674896749866 37.0 35.0 37.0 33.0 37.0 9 36.91098684041762 39.0 37.0 39.0 33.0 39.0 10-11 36.77282405150963 39.0 37.0 39.0 32.5 39.0 12-13 36.774436934048175 39.0 37.0 39.0 32.0 39.0 14-15 37.65032914988585 39.5 37.0 41.0 32.0 41.0 16-17 37.70257788651463 39.5 37.0 41.0 32.5 41.0 18-19 37.89002658209989 40.0 37.0 41.0 33.0 41.0 20-21 37.74737064874432 40.0 37.0 41.0 32.5 41.0 22-23 37.87581285432111 40.0 37.0 41.0 33.0 41.0 24-25 37.84130983377369 40.0 37.0 41.0 32.5 41.0 26-27 37.65856431008388 39.5 37.0 41.0 32.5 41.0 28-29 37.61441044814406 39.5 37.0 41.0 33.0 41.0 30-31 37.49673976476926 39.0 37.0 41.0 33.0 41.0 32-33 37.20579884180284 39.0 35.5 41.0 32.0 41.0 34-35 36.85501372393094 39.0 35.0 40.5 31.0 41.0 36-37 36.515166867608954 38.5 35.0 40.0 30.5 41.0 38-39 36.251416482825846 38.0 35.0 40.0 30.0 41.0 40-41 36.28923905933355 38.0 35.0 40.0 30.0 41.0 42-43 35.9826847600236 38.0 35.0 40.0 30.0 41.0 44-45 35.560695527537646 38.0 35.0 40.0 29.0 41.0 46-47 35.06407921401637 38.0 35.0 40.0 26.0 41.0 48-49 34.66958674293923 38.0 34.0 40.0 24.0 41.0 50-51 33.53857771336223 37.0 33.0 39.0 23.0 40.5 52-53 33.764171241310315 37.5 33.5 39.5 23.0 40.0 54-55 34.316601948285154 38.0 34.0 40.0 23.0 41.0 56-57 33.973954191570684 38.0 33.5 40.0 21.5 41.0 58-59 33.747284072544446 38.0 33.0 40.0 20.5 41.0 60-61 33.06004540440705 37.5 32.0 40.0 17.0 41.0 62-63 32.84902633840392 37.0 32.0 40.0 13.5 41.0 64-65 32.490894268014266 36.5 31.5 39.5 10.5 40.5 66-67 31.634508150988893 36.0 30.5 39.0 7.0 40.0 68-69 31.100353840135444 35.0 30.0 38.0 2.0 40.0 70-71 30.334996184234154 35.0 29.0 37.0 2.0 39.0 72-73 29.837677641536054 34.5 29.0 37.0 2.0 39.0 74-75 29.45797366800913 34.0 29.0 36.5 2.0 38.5 76-77 29.009948246671627 34.0 29.0 36.0 2.0 37.0 78-79 28.371958610163404 34.0 27.0 35.0 2.0 37.0 80-81 27.586213060821382 33.5 26.0 35.0 2.0 36.0 82-83 27.0765574096401 33.0 25.5 35.0 2.0 36.0 84-85 26.626763589256853 33.0 25.0 35.0 2.0 35.5 86-87 26.62012928712516 33.0 25.0 35.0 2.0 35.0 88-89 25.994516039042658 33.0 23.0 35.0 2.0 35.0 90-91 25.299534412436188 32.0 19.5 35.0 2.0 35.0 92-93 24.738989591616857 32.0 16.5 35.0 2.0 35.0 94-95 24.167413519995897 31.5 6.5 34.0 2.0 35.0 96-97 23.469533193956337 31.0 2.0 34.0 2.0 35.0 98-99 22.85830522278942 31.0 2.0 34.0 2.0 35.0 100-101 20.181556704204397 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.37371523773519044 1101 2 0.41502208816095276 1101 3 0.36709473554983063 1101 4 0.33178275532461754 1101 5 0.3985043600568261 1101 6 0.39292819346254504 1101 7 0.39684731019122665 1101 8 0.28492388156348625 1101 9 0.30923599150104053 1101 10-11 0.6525683417849848 1101 12-13 0.5325207071237372 1101 14-15 0.6427696905470341 1101 16-17 0.7100476070091588 1101 18-19 0.7121982019907804 1101 20-21 0.5584014907634582 1101 22-23 0.38822617410734495 1101 24-25 0.5241146214284171 1101 26-27 0.6394575042679307 1101 28-29 0.5066739879596582 1101 30-31 0.540147161736563 1101 32-33 0.5253207352730414 1101 34-35 0.5597191603135556 1101 36-37 0.5789542574432218 1101 38-39 0.8789679746534844 1101 40-41 0.7766809318815717 1101 42-43 0.9384436660129438 1101 44-45 1.0528843206099694 1101 46-47 1.1001210088095519 1101 48-49 1.0109427512112674 1101 50-51 1.3106597222784018 1101 52-53 1.3893152112613265 1101 54-55 1.1739258323106228 1101 56-57 1.0446642052981545 1101 58-59 1.2616401960224763 1101 60-61 1.4015072297281037 1101 62-63 1.5155263174730536 1101 64-65 1.274509116985307 1101 66-67 1.4384536768898037 1101 68-69 1.454650304431805 1101 70-71 1.319736236463541 1101 72-73 1.4312280503606836 1101 74-75 1.4827079713593854 1101 76-77 1.2080802517062388 1101 78-79 1.4484809500182152 1101 80-81 1.5350666505588073 1101 82-83 1.1909367625873273 1101 84-85 1.1231997339255742 1101 86-87 1.2223244221182092 1101 88-89 1.476315873168076 1101 90-91 1.2853185612951883 1101 92-93 1.2814127212663315 1101 94-95 1.288805025827525 1101 96-97 1.1235223626245272 1101 98-99 1.0984843648591855 1101 100-101 1.0417084971877166 1102 1 0.4269545183395067 1102 2 0.27022498871839673 1102 3 0.3019014248835141 1102 4 0.23412484263878497 1102 5 0.25014823150100085 1102 6 0.3555353104951209 1102 7 0.4203810055814188 1102 8 0.3398375469019257 1102 9 0.6042969411566759 1102 10-11 0.6630223487697009 1102 12-13 0.546799131920011 1102 14-15 0.6324297878818612 1102 16-17 0.39190809717054975 1102 18-19 0.44982749885175366 1102 20-21 0.2855024205213539 1102 22-23 0.41568044263454595 1102 24-25 0.5545881671752753 1102 26-27 0.2285293874251053 1102 28-29 0.24234940860715426 1102 30-31 0.5548243106157784 1102 32-33 0.5144654400633684 1102 34-35 0.578637400081341 1102 36-37 0.4801053007926086 1102 38-39 0.1697698844264508 1102 40-41 0.10865911616132706 1102 42-43 0.41657025257580926 1102 44-45 0.32629621925456576 1102 46-47 0.14999100680952182 1102 48-49 0.24027704866199429 1102 50-51 0.5115025557578932 1102 52-53 0.680953031330354 1102 54-55 0.5672344473149522 1102 56-57 0.3675345571497175 1102 58-59 0.2600196582706147 1102 60-61 0.31580591124627944 1102 62-63 0.287889482034565 1102 64-65 0.6020859515007118 1102 66-67 0.5905611452098221 1102 68-69 0.45376952165053197 1102 70-71 0.40273110305123083 1102 72-73 0.19497697983139162 1102 74-75 0.2969384467000289 1102 76-77 0.5147029176959741 1102 78-79 0.5682084330051289 1102 80-81 0.6127101527665317 1102 82-83 0.7491620173348359 1102 84-85 0.4939926179186642 1102 86-87 0.43611873690253944 1102 88-89 1.0128420761352999 1102 90-91 1.1582101955254558 1102 92-93 1.3262685395609743 1102 94-95 0.9067606866838496 1102 96-97 1.2324137814668674 1102 98-99 1.0720698559180235 1102 100-101 1.3345694129966752 1103 1 0.11081747064791614 1103 2 -0.01765010156456981 1103 3 0.10530025496419171 1103 4 0.1352486652626439 1103 5 0.047505190006582154 1103 6 0.15919297228342089 1103 7 0.22339775076036972 1103 8 0.2897992151585598 1103 9 -0.029224476550865575 1103 10-11 0.3268702148871512 1103 12-13 -0.004167296374973262 1103 14-15 0.32450756557747695 1103 16-17 0.4165895492903786 1103 18-19 0.4424421674608965 1103 20-21 0.26004200821476786 1103 22-23 0.2955973206804501 1103 24-25 0.4074756197536118 1103 26-27 0.45822157489814686 1103 28-29 0.2915927572485373 1103 30-31 0.12903875638313878 1103 32-33 0.1875597608026851 1103 34-35 0.38631681359334635 1103 36-37 0.3125068399785036 1103 38-39 0.4371812468919103 1103 40-41 0.47258758986772875 1103 42-43 0.47542488529362004 1103 44-45 0.4085973070872626 1103 46-47 0.5681927920087873 1103 48-49 0.7009937906363675 1103 50-51 0.8170620143312632 1103 52-53 0.6741445248879145 1103 54-55 0.7334660302064222 1103 56-57 0.6577995268664125 1103 58-59 0.5783629381367135 1103 60-61 0.6608988318293214 1103 62-63 0.8051067207193014 1103 64-65 0.7721186628984213 1103 66-67 0.7203036199860513 1103 68-69 0.5863948886369172 1103 70-71 0.6051904972864364 1103 72-73 0.46345818002169636 1103 74-75 0.6714445095246475 1103 76-77 0.9990571572360913 1103 78-79 0.965243947180479 1103 80-81 1.2435865569285447 1103 82-83 0.93906067855864 1103 84-85 0.7907974021146416 1103 86-87 0.6624251591334733 1103 88-89 0.4778763774992214 1103 90-91 0.6470114103089415 1103 92-93 0.45934624576972993 1103 94-95 0.38662727742654823 1103 96-97 0.27247846474313775 1103 98-99 0.5222718679031644 1103 100-101 0.959975840404244 1104 1 0.23601067002967824 1104 2 0.2930145043086938 1104 3 0.34023070318675863 1104 4 0.48300755243420923 1104 5 0.38599050685356673 1104 6 0.5254990001041335 1104 7 0.6082509192302297 1104 8 0.5525503743548512 1104 9 0.5766487846547008 1104 10-11 0.7573184374528381 1104 12-13 0.6751217299001411 1104 14-15 0.39019535537654804 1104 16-17 0.36944890014046194 1104 18-19 0.3512520592692354 1104 20-21 0.32186581037860407 1104 22-23 0.6611305509741072 1104 24-25 0.6238589273270279 1104 26-27 0.46208556253338173 1104 28-29 0.5713454620398863 1104 30-31 0.7418671953321336 1104 32-33 0.6102800080979023 1104 34-35 0.5161467981373917 1104 36-37 0.2862317240588723 1104 38-39 0.3568103040789268 1104 40-41 0.3775334940408257 1104 42-43 0.6717154571637849 1104 44-45 0.7949960706112122 1104 46-47 0.5280073206022919 1104 48-49 0.6144404676069968 1104 50-51 0.3927961719819564 1104 52-53 0.6849636902665779 1104 54-55 0.532538673173967 1104 56-57 0.7018489859081427 1104 58-59 0.7221032781676229 1104 60-61 0.8788277344568343 1104 62-63 0.9596662261288813 1104 64-65 0.7844834233311957 1104 66-67 0.8748631253956454 1104 68-69 0.983612817539548 1104 70-71 0.848621718306525 1104 72-73 1.095606557146887 1104 74-75 1.18921885264675 1104 76-77 0.7973570026765877 1104 78-79 1.0958467292515799 1104 80-81 1.0959822292623933 1104 82-83 0.7906835533654437 1104 84-85 0.6810601532738403 1104 86-87 0.6709259319309986 1104 88-89 0.7947233635888651 1104 90-91 0.47854154941249405 1104 92-93 0.3789753029567464 1104 94-95 0.5435051754172733 1104 96-97 1.039866409101716 1104 98-99 1.2742038617207818 1104 100-101 1.0952154076376281 1105 1 0.3964467052748475 1105 2 0.2786138543182659 1105 3 0.5120952698364611 1105 4 0.4241511993848093 1105 5 0.3454011261655694 1105 6 0.33920810137096424 1105 7 0.4815074553229053 1105 8 0.4668679077532403 1105 9 0.6944712233328687 1105 10-11 0.5021715125332733 1105 12-13 0.5417662944018815 1105 14-15 0.5009797331928212 1105 16-17 0.5729059433377373 1105 18-19 0.42511142412772784 1105 20-21 0.44607258391390303 1105 22-23 0.2752512661503559 1105 24-25 0.30108638195144266 1105 26-27 0.05529521713258134 1105 28-29 0.16475240180837147 1105 30-31 0.17174924448298867 1105 32-33 0.2980546313882826 1105 34-35 0.3216434664695029 1105 36-37 0.3752890511600171 1105 38-39 0.14205313994234103 1105 40-41 0.4839355728895285 1105 42-43 0.1367562916917251 1105 44-45 0.06335876713759347 1105 46-47 0.41983084259783965 1105 48-49 0.6949923524391579 1105 50-51 0.9057165995187617 1105 52-53 1.0522386334157687 1105 54-55 0.888179628174278 1105 56-57 0.6217301945541394 1105 58-59 0.7999655474373668 1105 60-61 0.9704229192978602 1105 62-63 0.9553656429119002 1105 64-65 1.038293712044613 1105 66-67 1.2103132857919867 1105 68-69 1.021994705593631 1105 70-71 1.0248755881310032 1105 72-73 0.8352531715466043 1105 74-75 1.0396264975054272 1105 76-77 0.7269915698399281 1105 78-79 0.7234855643118401 1105 80-81 0.8522264799756556 1105 82-83 1.0090000221243436 1105 84-85 1.0135276887735003 1105 86-87 0.9940650308163974 1105 88-89 0.9653119971614288 1105 90-91 0.7119415783632164 1105 92-93 0.6212870181937014 1105 94-95 0.4824086434337289 1105 96-97 0.6004096087169515 1105 98-99 0.8084614391055993 1105 100-101 0.884594227848968 1106 1 0.3876362054852436 1106 2 0.25590087283757157 1106 3 0.4359850333926225 1106 4 0.2566855123454843 1106 5 0.2597475605962529 1106 6 0.38805472255493356 1106 7 0.3883482989749538 1106 8 0.4208627732199588 1106 9 0.5245895634507818 1106 10-11 0.4027809428648439 1106 12-13 0.4530821249339212 1106 14-15 0.3821396365539087 1106 16-17 0.29031394961642576 1106 18-19 0.17663972788785287 1106 20-21 0.22120863013415715 1106 22-23 0.47606186995445654 1106 24-25 0.4991748793676223 1106 26-27 0.37792924216224577 1106 28-29 0.37132635374422307 1106 30-31 0.4391905826766376 1106 32-33 0.22729050314788424 1106 34-35 0.25726204534795016 1106 36-37 0.3933107429083549 1106 38-39 0.6085947528287861 1106 40-41 0.4704385112987737 1106 42-43 0.6152359308560165 1106 44-45 0.1687833589989367 1106 46-47 0.44990639493840234 1106 48-49 0.642546593629838 1106 50-51 0.34298640836851035 1106 52-53 0.18323305918487875 1106 54-55 0.258988908157626 1106 56-57 0.3745468595660313 1106 58-59 0.5886282048183702 1106 60-61 0.3418858357694958 1106 62-63 0.5399766996518025 1106 64-65 0.5229102059053901 1106 66-67 0.49175993818029795 1106 68-69 0.23919231893396642 1106 70-71 0.41978076947080467 1106 72-73 0.3868774523100704 1106 74-75 0.720229923364375 1106 76-77 0.9638833805902998 1106 78-79 0.9909458142302832 1106 80-81 0.9535649572981306 1106 82-83 0.835889185233242 1106 84-85 1.074774491510027 1106 86-87 0.8765759405176361 1106 88-89 0.8524763731454321 1106 90-91 0.8053974123939227 1106 92-93 0.4909958721904566 1106 94-95 0.24977197076259117 1106 96-97 0.4932167705871322 1106 98-99 0.8762457887077453 1106 100-101 1.2397067762511647 1107 1 -0.007795023866172812 1107 2 0.11240408966942539 1107 3 -0.013393335586687982 1107 4 0.14381383785409696 1107 5 0.14865818483551863 1107 6 0.18904585453197598 1107 7 0.23624431705876248 1107 8 0.43529504586819456 1107 9 0.2580272264659058 1107 10-11 0.4232230398914467 1107 12-13 0.11578209015736007 1107 14-15 0.04136194571753293 1107 16-17 0.08772961645697563 1107 18-19 0.1163132629252388 1107 20-21 0.3580000577119762 1107 22-23 0.35089885482664585 1107 24-25 0.33299725808436875 1107 26-27 0.3599975781038225 1107 28-29 0.38969272481011785 1107 30-31 0.17819249538747073 1107 32-33 -0.1580011852541361 1107 34-35 -0.029970609842457918 1107 36-37 0.44857287123386413 1107 38-39 0.2324101762334081 1107 40-41 0.3093686973533707 1107 42-43 0.19459169507094032 1107 44-45 0.056791966197685895 1107 46-47 -0.14263185927825361 1107 48-49 -0.2964314101955807 1107 50-51 -0.10472920630720495 1107 52-53 -0.06877746072469648 1107 54-55 -0.04977261157807078 1107 56-57 -0.05535663547831149 1107 58-59 -0.049445608431490484 1107 60-61 0.013975472354438523 1107 62-63 -0.06933037737828585 1107 64-65 -0.013713859474535184 1107 66-67 -0.2493068328160497 1107 68-69 -0.2679912143645886 1107 70-71 -0.28196675269925464 1107 72-73 -0.5060759470987293 1107 74-75 -0.28933363080011887 1107 76-77 -0.010881877625660508 1107 78-79 0.0866900326270823 1107 80-81 0.022487753333603422 1107 82-83 -0.251443452568882 1107 84-85 -0.44511335408204644 1107 86-87 -0.39271418293915517 1107 88-89 -0.436353830297449 1107 90-91 -0.2803050741301618 1107 92-93 -0.16666149174625033 1107 94-95 -0.16970942377749054 1107 96-97 0.13637665582448122 1107 98-99 0.2617222275879385 1107 100-101 -0.10035364802287816 1108 1 0.13035580812523762 1108 2 0.19069563172908488 1108 3 0.2198038885525655 1108 4 0.45566683992448986 1108 5 0.2996147832558833 1108 6 0.20106563829089197 1108 7 0.37561246367328494 1108 8 0.34128385040772713 1108 9 0.2449722460196213 1108 10-11 0.2169169667375499 1108 12-13 0.12055070452741745 1108 14-15 0.48201546155220854 1108 16-17 0.5731170484023806 1108 18-19 0.6877753823946122 1108 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-3.0614863713277813 2316 72-73 -2.722048933286235 2316 74-75 -2.5250016420456873 2316 76-77 -2.3102493143693366 2316 78-79 -3.403699284082368 2316 80-81 -3.4129266214372045 2316 82-83 -3.449035433989245 2316 84-85 -3.581847860866059 2316 86-87 -3.3054922663030446 2316 88-89 -3.885542499164334 2316 90-91 -3.6880639055688604 2316 92-93 -4.5567039682161194 2316 94-95 -3.7342415714367014 2316 96-97 -4.046081173291764 2316 98-99 -4.862574924349477 2316 100-101 -5.745743881490668 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 895.0 3 285.0 4 247.0 5 227.0 6 290.0 7 336.0 8 479.0 9 632.0 10 966.0 11 1437.0 12 2365.0 13 3305.0 14 4008.0 15 5340.0 16 7330.0 17 10398.0 18 7697.0 19 5265.0 20 5481.0 21 5968.0 22 6837.0 23 7613.0 24 8430.0 25 9286.0 26 10337.0 27 11841.0 28 13916.0 29 16184.0 30 19723.0 31 23598.0 32 29502.0 33 36892.0 34 48327.0 35 66780.0 36 81746.0 37 94418.0 38 66515.0 39 8830.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.26785385008843 19.291409916605335 14.491526646687863 15.949209586618371 2 13.405842417012215 26.708260776145444 33.89903887782492 25.98685792901742 3 28.910767257275715 26.80878697987653 30.68195301852 13.598492744327748 4 10.054616243120854 16.2929906482111 45.35938321809295 28.29300989057509 5 8.879598045394697 43.549144980458756 35.047702440690685 12.523554533455858 6 23.622338992185394 40.0395863007019 16.183065788967163 20.15500891814554 7 22.01441086683256 37.74611349118801 22.38189759113414 17.857578050845284 8 22.8463524544157 24.049104350754526 21.104446974678062 32.00009622015171 9 20.89874197294055 24.373552563206076 27.844995156499593 26.88271030735378 10-11 24.687927530862417 24.030181573810772 26.219460362826847 25.062430532499963 12-13 32.61614946676289 24.900649506855906 24.142891508299254 18.340309518081952 14-15 25.001543537788613 26.25457548701624 33.517141287831706 15.226739687363436 16-17 17.088480212330058 30.424299282744578 31.78431820642042 20.70290229850495 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32.12298743318166 26.715712210560742 24.748439168454368 44-45 19.674645152704983 26.451815641338488 28.68342955722048 25.190109648736048 46-47 24.039899089901215 30.220762886118607 28.964196474880293 16.77514154909988 48-49 21.40163069052232 28.591996293229293 29.81585590409853 20.190517112149855 50-51 14.280755479309295 30.42688108255439 36.3842170239374 18.908146414198907 52-53 18.84307763347023 34.34908887433432 26.182827980789042 20.625005511406407 54-55 15.804709658696451 27.972797688621238 30.627532279295878 25.59496037338644 56-57 21.87163061837497 26.695803509086957 27.815370111916575 23.61719576062149 58-59 26.669916136423215 26.54509481049525 26.703426293287624 20.081562759793904 60-61 20.910275166843697 28.4082810589815 34.14746377537728 16.533979998797523 62-63 18.608105649039523 29.678841092756347 31.963900786483794 19.749152471720333 64-65 17.250871778427992 35.24838670888612 28.832899114192955 18.667842398492926 66-67 20.181742962150512 28.340372786340573 25.820761736163227 25.657122515345687 68-69 17.269151346257942 29.472523506886667 29.364181318791477 23.894143828063914 70-71 24.048486753883367 28.516123754467237 26.377625002406052 21.057764489243343 72-73 21.02516121118244 24.080537041892057 34.53867905846095 20.35562268846455 74-75 16.86539183203487 35.208994191398396 28.575369091568064 19.35024488499867 76-77 18.805691307886285 27.356604779640225 28.685779347560263 25.151924564913227 78-79 19.44348869420992 27.903923441599115 31.201301852123652 21.451286012067317 80-81 23.553124308089217 27.395014070858082 28.228916977124026 20.822944643928672 82-83 20.009206825561936 25.54252502804954 34.14328803970804 20.304980106680482 84-85 18.881925129745337 28.898994669076234 31.20045604292961 21.018624158248823 86-87 18.798232539269556 30.66837821607055 30.07938499678884 20.45400424787105 88-89 18.128163578823976 29.451821395554695 26.165026864111134 26.254988161510195 90-91 19.64083803389537 24.52233896331557 29.573765811771157 26.263057191017904 92-93 23.403366499060674 29.061451877890043 29.965016826080788 17.5701647969685 94-95 20.844333734465483 27.028717160144726 30.32799568348669 21.798953421903096 96-97 14.1971072127257 27.965106956159197 35.744283377697954 22.093502453417145 98-99 19.083763146012267 31.02567166668939 26.777082609849856 23.113482577448487 100-101 17.2977933361302 26.09773686401692 30.435420849600487 26.1690489502524 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 760.0 1 604.0 2 685.5 3 918.0 4 794.5 5 760.0 6 1202.5 7 2249.5 8 3391.5 9 3704.0 10 2944.5 11 2095.0 12 1827.5 13 1749.0 14 1670.5 15 1509.0 16 1342.0 17 1277.0 18 1284.5 19 1304.5 20 1318.5 21 1395.0 22 1518.5 23 1712.5 24 1851.0 25 2186.0 26 3086.5 27 3882.5 28 4032.0 29 4584.0 30 5856.5 31 7113.0 32 9251.0 33 12218.5 34 15319.0 35 17290.5 36 19400.5 37 21414.5 38 20065.0 39 17832.5 40 15736.0 41 13770.5 42 11874.0 43 10832.0 44 10884.5 45 10975.5 46 15036.0 47 45998.5 48 113677.0 49 107473.5 50 38932.0 51 11196.0 52 3892.0 53 1953.5 54 1324.5 55 1061.0 56 908.5 57 752.5 58 620.5 59 524.0 60 457.0 61 420.5 62 374.5 63 331.5 64 275.0 65 280.5 66 272.0 67 187.0 68 118.5 69 83.5 70 70.0 71 57.5 72 48.0 73 74.5 74 73.0 75 38.0 76 24.5 77 22.5 78 20.0 79 13.5 80 10.0 81 7.5 82 7.0 83 6.5 84 4.0 85 2.5 86 2.5 87 2.0 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010741861837211092 2 0.013146756278377756 3 0.012505451094066644 4 0.016673934792088858 5 0.028057101813611065 6 0.0448913629017777 7 0.00513044147448888 8 0.025331554780288843 9 0.033989174768488825 10-11 0.037676679578277715 12-13 0.028538080701844395 14-15 0.02573237052048329 16-17 0.027335633481261064 18-19 0.022846497191083292 20-21 0.04873919400764436 22-23 0.011864145909755534 24-25 0.0030461996254777723 26-27 9.619577764666649E-4 28-29 8.016314803888875E-5 30-31 0.0026453838852833287 32-33 0.0018437524048944413 34-35 0.0 36-37 2.4048944411666622E-4 38-39 8.016314803888875E-5 40-41 0.0012024472205833313 42-43 0.0035271785137111046 44-45 0.0014429366646999974 46-47 7.214683323499987E-4 48-49 7.214683323499987E-4 50-51 0.002885873329399995 52-53 0.003447015365672216 54-55 0.004970115178411102 56-57 7.214683323499987E-4 58-59 0.0058519098068388785 60-61 0.002485057589205551 62-63 4.008157401944437E-4 64-65 4.8097888823333244E-4 66-67 0.01603262960777775 68-69 0.03855847420670548 70-71 0.04809788882333325 72-73 0.014349203498961084 74-75 0.027255470333222172 76-77 0.023327476079316626 78-79 0.049460662339994355 80-81 0.07278813841931098 82-83 0.04449054716158325 84-85 0.016433445347972194 86-87 0.019479644973449964 88-89 0.020281276453838853 90-91 0.03543211143318883 92-93 0.022846497191083292 94-95 0.012264961649949979 96-97 0.01731523997639997 98-99 0.0476970730831388 100-101 0.06597427083600543 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 623728.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.78813200625914 #Duplication Level Percentage of deduplicated Percentage of total 1 75.05559786747905 11.849876869404612 2 8.91901497842092 2.8162917169022395 3 3.7491749174917497 1.7757740553574635 4 2.2452399086062456 1.4179257625118642 5 1.4785478547854785 1.1671754354462203 6 1.0662604722010662 1.0100556652899984 7 0.7717694846407718 0.8529358951337763 8 0.6722518405686723 0.8490880640279096 9 0.5239908606245239 0.7445553189851987 >10 4.489464331048489 14.598831541954185 >50 0.5859355166285859 6.383391478336711 >100 0.3716679360243717 10.622579072929225 >500 0.02538715410002539 2.7534438088397506 >1k 0.027418126428027417 10.461130492778905 >5k 0.011170347804011169 11.869917656414335 >10k+ 0.007108403148007108 20.827027165687607 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 36952 5.924377292666034 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 25576 4.100505348485237 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 19384 3.1077649231716387 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 15036 2.4106661878254623 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11474 1.839583921196419 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 11343 1.8185811764102302 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 10139 1.6255483159325859 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9435 1.5126786034938307 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9349 1.4988905420311418 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7986 1.280365800477131 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7606 1.2194418079675755 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6652 1.0664905215093758 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6273 1.0057268552958982 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5868 0.9407947053843982 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5361 0.8595092732729651 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5333 0.8550201369827873 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5127 0.8219929199907652 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5046 0.8090064900084651 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4810 0.7711694841341097 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4795 0.768764589692943 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4268 0.6842726316599543 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4126 0.6615062976169099 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3869 0.6203024395249211 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3537 0.567074109227099 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3406 0.5460713644409101 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3374 0.5409409229664213 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3174 0.5088756637508658 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3015 0.48338378267449916 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2715 0.43528589385116595 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2076 0.3328373906574661 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1998 0.32033193956339945 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1950 0.3126362773516661 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1920 0.3078264884693328 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1802 0.288907985532155 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1647 0.26405740964009955 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1574 0.25235359002642177 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1507 0.24161172818921067 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1498 0.24016879152451068 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1347 0.21595952081676625 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1301 0.20858451119718852 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1208 0.19367416566195522 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1139 0.18261165123258857 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1131 0.18132904086396634 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1047 0.16786163199343304 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1015 0.16273119051894416 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 975 0.15631813867583305 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 916 0.1468588872072442 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 878 0.14076648795628863 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 835 0.1338724572249442 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 828 0.13275017315239976 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 796 0.12761973167791088 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 790 0.12665777390144423 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 766 0.12280994279557755 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 765 0.12264961649949978 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 736 0.11800015391324424 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 716 0.11479362799168867 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 671 0.1075789446681887 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 653 0.10469307133878872 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 630 0.10100556652899982 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.4048944411666625E-4 0.0 16-17 0.0 0.0 0.0 5.611420362722213E-4 0.0 18-19 0.0 0.0 0.0 8.817946284277762E-4 0.0 20-21 0.0 0.0 0.0 9.61957776466665E-4 0.0 22-23 0.0 0.0 0.0 0.0013627735166611087 0.0 24-25 0.0 0.0 0.0 0.001843752404894441 0.0 26-27 0.0 0.0 0.0 0.003447015365672216 0.0 28-29 0.0 0.0 0.0 0.0058519098068388785 0.0 30-31 0.0 0.0 0.0 0.01026088294897776 0.0 32-33 0.0 0.0 0.0 0.014269040350922198 0.0 34-35 0.0 0.0 0.0 0.018357360900905523 0.0 36-37 0.0 0.0 0.0 0.022766334043044404 0.0 38-39 0.0 0.0 0.0 0.02869840699792217 0.0 40-41 0.0 0.0 0.0 0.03631390606161661 0.0 42-43 0.0 0.0 0.0 0.04865903085960547 0.0 44-45 0.0 0.0 0.0 0.07671613267321653 0.0 46-47 0.0 0.0 0.0 0.14204909832491086 0.0 48-49 0.0 0.0 0.0 0.18213067234435523 0.0 50-51 0.0 0.0 0.0 0.23103019264807736 0.0 52-53 0.0 0.0 0.0 0.28586178590667727 0.0 54-55 0.0 0.0 0.0 0.3584094348818716 0.0 56-57 0.0 0.0 0.0 0.4495549342020881 0.0 58-59 0.0 0.0 0.0 0.5499993586948158 0.0 60-61 0.0 0.0 0.0 0.681547084626632 0.0 62-63 0.0 0.0 0.0 0.825760587948593 0.0 64-65 0.0 0.0 0.0 0.9220365287432983 0.0 66-67 0.0 0.0 0.0 1.1068927481209758 0.0 68-69 0.0 0.0 0.0 1.3469012133494087 0.0 70-71 0.0 0.0 0.0 1.4945617320370417 0.0 72-73 0.0 0.0 0.0 1.7104410897057694 0.0 74-75 0.0 0.0 0.0 1.9245568581176409 0.0 76-77 0.0 0.0 0.0 2.0541005053484853 0.0 78-79 0.0 0.0 0.0 2.302926916861196 0.0 80-81 0.0 0.0 0.0 2.541492445424929 0.0 82-83 0.0 0.0 0.0 2.7753283482543676 0.0 84-85 0.0 0.0 0.0 3.170292178641972 0.0 86-87 0.0 0.0 0.0 3.6578444450144936 0.0 88-89 0.0 0.0 0.0 4.264278659928687 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATAGT 20 0.0020720542 71.26366 6 CTTATGT 130 0.0 54.8138 2 GCTTATG 135 0.0 52.78366 1 CATATAG 125 0.0 49.405502 3 GGAACAC 50 2.820957E-5 47.512917 8 GTATGGT 40 5.383312E-4 47.505295 1 TATGTAC 165 0.0 43.18663 4 TAGGCAT 115 5.4569682E-11 41.30895 5 CTTTAGG 115 5.4569682E-11 41.30895 2 GACATAT 150 0.0 41.171253 1 ACATATA 150 0.0 41.171253 2 AGGCTTA 215 0.0 39.775066 6 CCTTTAG 120 8.731149E-11 39.587746 1 GCGAGTA 25 0.0016207212 37.976814 32-33 GGGAACA 90 5.6802673E-7 36.954494 7 AAGACAT 90 5.687416E-7 36.948563 5 GGAGGCG 65 1.3253649E-4 36.5484 8 GAGGCGC 65 1.3253649E-4 36.5484 9 CATTCCG 145 1.6370905E-11 36.04428 9 GTAAGCG 195 0.0 35.33579 94-95 >>END_MODULE