##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936650_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 642781 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.639636205799487 31.0 31.0 34.0 30.0 34.0 2 31.586457907125443 31.0 31.0 34.0 30.0 34.0 3 31.66729103691615 33.0 31.0 34.0 30.0 34.0 4 35.20678271448596 37.0 35.0 37.0 33.0 37.0 5 35.19073992541783 37.0 35.0 37.0 33.0 37.0 6 35.42301343692486 37.0 35.0 37.0 33.0 37.0 7 35.321114034173384 37.0 35.0 37.0 33.0 37.0 8 35.353306958357514 37.0 35.0 37.0 33.0 37.0 9 36.78262580879024 39.0 37.0 39.0 33.0 39.0 10-11 36.66447125848461 39.0 37.0 39.0 32.0 39.0 12-13 36.64805042463919 39.0 37.0 39.0 32.0 39.0 14-15 37.46638901896603 39.0 37.0 41.0 32.0 41.0 16-17 37.56261541644822 39.0 37.0 41.0 32.0 41.0 18-19 37.754705724033535 40.0 37.0 41.0 32.0 41.0 20-21 37.65274331381917 40.0 37.0 41.0 32.0 41.0 22-23 37.530055337665544 39.5 37.0 41.0 32.0 41.0 24-25 37.2671391967093 39.0 36.5 41.0 31.5 41.0 26-27 36.982432585904064 39.0 36.0 40.5 30.5 41.0 28-29 37.227547173920826 39.0 36.0 41.0 31.5 41.0 30-31 37.26990841359654 39.0 36.0 41.0 32.0 41.0 32-33 37.01089017877006 39.0 35.0 41.0 31.0 41.0 34-35 36.66032832333252 39.0 35.0 40.0 30.5 41.0 36-37 36.30131179981984 38.5 35.0 40.0 30.0 41.0 38-39 36.03669834671529 38.0 35.0 40.0 30.0 41.0 40-41 36.14312028513599 38.0 35.0 40.0 30.0 41.0 42-43 35.81902389771944 38.0 35.0 40.0 30.0 41.0 44-45 35.38727887103072 38.0 35.0 40.0 28.0 41.0 46-47 34.81727057893746 38.0 34.0 40.0 25.0 41.0 48-49 34.306035025926406 38.0 33.5 40.0 23.0 41.0 50-51 33.413587209329464 37.0 32.0 39.5 23.0 40.5 52-53 33.72584752816278 37.5 33.0 39.5 23.0 40.5 54-55 34.20112760022465 38.0 34.0 40.0 22.5 41.0 56-57 33.89391876860081 38.0 33.5 40.0 21.0 41.0 58-59 33.64951826516341 38.0 33.0 40.0 20.5 41.0 60-61 32.93959995706158 37.0 32.0 40.0 14.0 41.0 62-63 32.7730945687567 37.0 31.5 40.0 13.5 41.0 64-65 32.48222645037734 36.5 31.5 39.0 10.5 40.5 66-67 31.721317991664346 36.0 31.0 39.0 7.5 40.0 68-69 31.188058607830662 35.0 30.0 38.5 4.0 40.0 70-71 30.43956728652527 35.0 29.5 37.0 2.0 39.0 72-73 29.915223847624617 34.5 29.0 37.0 2.0 39.0 74-75 29.546429499316254 34.0 29.0 36.5 2.0 38.5 76-77 29.141076043006873 34.0 29.0 36.0 2.0 37.5 78-79 28.539102742613736 34.0 28.0 35.5 2.0 37.0 80-81 27.810800257008218 34.0 26.0 35.0 2.0 36.5 82-83 27.309662233326748 33.5 25.5 35.0 2.0 36.0 84-85 26.816743183137024 33.0 25.0 35.0 2.0 36.0 86-87 26.831577162361675 33.0 25.0 35.0 2.0 35.0 88-89 26.187454980778835 33.0 24.0 35.0 2.0 35.0 90-91 25.559157006818808 32.0 20.0 35.0 2.0 35.0 92-93 25.131641258842436 32.0 19.5 35.0 2.0 35.0 94-95 24.617547811774152 32.0 15.5 35.0 2.0 35.0 96-97 23.990057266782934 31.0 4.0 34.0 2.0 35.0 98-99 23.441267710153223 31.0 2.0 34.0 2.0 35.0 100-101 21.481128875931304 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3196133996978041 1101 2 0.4146524600247865 1101 3 0.2559502776342022 1101 4 0.22298904926234542 1101 5 0.5364227563177124 1101 6 0.3297013617086719 1101 7 0.4925685153668411 1101 8 0.4357709175291191 1101 9 0.679709395499259 1101 10-11 0.5718822902349459 1101 12-13 0.7980121632533042 1101 14-15 0.7857599840098644 1101 16-17 0.696398046390506 1101 18-19 0.802151792324004 1101 20-21 0.6926326825125315 1101 22-23 0.7197349478413315 1101 24-25 0.9530156574458815 1101 26-27 0.8703691392974875 1101 28-29 0.8783805274617933 1101 30-31 0.7548198979135492 1101 32-33 0.6669113826199933 1101 34-35 0.7069998638834178 1101 36-37 0.8726603277466225 1101 38-39 0.7342681446712689 1101 40-41 0.8554564658411223 1101 42-43 0.622953399400437 1101 44-45 0.8865867002110477 1101 46-47 1.1517996179955787 1101 48-49 1.077578318772602 1101 50-51 0.923128285221722 1101 52-53 0.7428005748519269 1101 54-55 0.9897581300529836 1101 56-57 0.6650383831453865 1101 58-59 0.8101260102469681 1101 60-61 0.9522903829691671 1101 62-63 1.182624546481975 1101 64-65 1.391157316393155 1101 66-67 1.4290026669246991 1101 68-69 1.3364276423562131 1101 70-71 1.3250698091630966 1101 72-73 1.3148763031982114 1101 74-75 1.535513639580774 1101 76-77 1.3860788202716243 1101 78-79 1.5197220063710795 1101 80-81 1.3822256935937602 1101 82-83 1.2459542979996563 1101 84-85 1.4837825987191913 1101 86-87 1.4180645782522099 1101 88-89 1.5590377524085035 1101 90-91 1.3936729588879153 1101 92-93 1.3677013508473514 1101 94-95 1.2568603118178885 1101 96-97 1.0409441472640424 1101 98-99 1.5041650707991217 1101 100-101 1.6965360200736974 1102 1 0.12165030360386453 1102 2 0.31596087123974215 1102 3 0.3429668125012064 1102 4 0.17779374612311472 1102 5 0.15837339135245543 1102 6 0.06661485320159954 1102 7 -0.08515015225837175 1102 8 0.035224548010788226 1102 9 0.2508428726140437 1102 10-11 0.2972573201894164 1102 12-13 0.5260207901404357 1102 14-15 0.40574081314957056 1102 16-17 0.44170597833394964 1102 18-19 0.3319720655087579 1102 20-21 -0.12268004214599415 1102 22-23 -0.3863901420220799 1102 24-25 -0.13368695458383684 1102 26-27 -0.09812594816956732 1102 28-29 -0.04940284181690657 1102 30-31 0.16444606613784174 1102 32-33 0.13759194816037024 1102 34-35 0.04003365252469138 1102 36-37 -0.2304573334084168 1102 38-39 -0.30936473335413694 1102 40-41 -0.5140906225844688 1102 42-43 -0.14440341823675595 1102 44-45 0.09067488616838659 1102 46-47 0.18848305916500152 1102 48-49 0.35370101227847073 1102 50-51 0.058527278751554945 1102 52-53 0.141810879189336 1102 54-55 -0.0017419897648949245 1102 56-57 0.2971399887049273 1102 58-59 0.24754992589518565 1102 60-61 -0.34630774787195406 1102 62-63 -0.22444231189329855 1102 64-65 0.1364916082695018 1102 66-67 0.08017448576007169 1102 68-69 -0.10483763442308458 1102 70-71 0.06069885301644007 1102 72-73 0.07399444362476615 1102 74-75 0.07593300214966803 1102 76-77 -0.05532065252971563 1102 78-79 0.0264102402555757 1102 80-81 0.05867908443967451 1102 82-83 0.3325562630128367 1102 84-85 -0.015893893013373628 1102 86-87 -0.23864282649257618 1102 88-89 -0.05045661615128694 1102 90-91 -0.02390192728510243 1102 92-93 -0.09495860601821349 1102 94-95 -0.22655449767168534 1102 96-97 -0.09673378228304585 1102 98-99 0.009032072955843518 1102 100-101 0.9019398063186053 1103 1 0.2785408450865532 1103 2 0.3642388168590749 1103 3 0.17951805581981972 1103 4 0.3915051102876532 1103 5 0.4316943302323111 1103 6 0.0728802494367855 1103 7 0.26554006386991347 1103 8 -0.012700155684306935 1103 9 0.2878542600181859 1103 10-11 0.3686865556065584 1103 12-13 0.18851672321039104 1103 14-15 0.35670990448119966 1103 16-17 0.5043669125658781 1103 18-19 0.24242058979414338 1103 20-21 0.23359464992930867 1103 22-23 0.12388408526120998 1103 24-25 0.17259006145148703 1103 26-27 0.22678225540933283 1103 28-29 0.431782377797326 1103 30-31 0.3634188758055217 1103 32-33 0.43368769531352314 1103 34-35 0.4901266109257989 1103 36-37 0.5685573075397556 1103 38-39 0.42474635193519816 1103 40-41 0.31389450407136366 1103 42-43 0.38888266124268256 1103 44-45 0.08393307812748674 1103 46-47 0.10136244563955188 1103 48-49 0.4834686162756796 1103 50-51 -0.04964608866652753 1103 52-53 0.4114436935049284 1103 54-55 0.6197616123731535 1103 56-57 0.9643814481518262 1103 58-59 0.6584097911054982 1103 60-61 1.1328463306778929 1103 62-63 0.8212444182949383 1103 64-65 0.6550065978116848 1103 66-67 0.6867884759994389 1103 68-69 0.8242583451260685 1103 70-71 0.9850353624284764 1103 72-73 0.9169412249401248 1103 74-75 0.8058028603881056 1103 76-77 0.7074169723831325 1103 78-79 0.8854597406041691 1103 80-81 1.1076588659874318 1103 82-83 1.147734045954973 1103 84-85 0.9109362255085891 1103 86-87 0.7063583354874368 1103 88-89 0.6950976483153646 1103 90-91 0.9411666295357186 1103 92-93 1.031111577574631 1103 94-95 0.849308853478675 1103 96-97 0.9057830664271549 1103 98-99 1.0988159446732482 1103 100-101 1.3944682748289452 1104 1 -0.009978792375683554 1104 2 -0.1357611831409251 1104 3 0.09039418875033434 1104 4 0.21325737614867535 1104 5 0.14015354473382047 1104 6 0.047200491128627675 1104 7 -0.07131794217238507 1104 8 0.016000750660104757 1104 9 0.0099086406828377 1104 10-11 0.06204002992680557 1104 12-13 0.06918137577836347 1104 14-15 0.1323896627893575 1104 16-17 -0.09533097263049228 1104 18-19 -0.15637095099135934 1104 20-21 0.07120794298066357 1104 22-23 0.08687502181710727 1104 24-25 0.08875320344544946 1104 26-27 -0.060982095043840445 1104 28-29 -0.28498499380388154 1104 30-31 -0.26271405470807707 1104 32-33 -0.12069296933903928 1104 34-35 -0.3437102470802387 1104 36-37 -0.07570251119135918 1104 38-39 -0.22404518885030456 1104 40-41 -0.2676764929074906 1104 42-43 -0.07939528437665899 1104 44-45 -0.12981314543746691 1104 46-47 0.11269175077550386 1104 48-49 -0.15701476741011078 1104 50-51 0.17467415302532174 1104 52-53 0.1788152947133952 1104 54-55 0.36674046433689966 1104 56-57 0.3465566747379327 1104 58-59 0.20674815968556004 1104 60-61 0.10414542433348117 1104 62-63 -0.16364984152379236 1104 64-65 -0.05496319070795863 1104 66-67 0.10446219201152473 1104 68-69 0.28649399467289527 1104 70-71 0.13005046816865473 1104 72-73 0.09821010711535294 1104 74-75 0.01954908395305921 1104 76-77 0.06617830168827155 1104 78-79 0.32503677987909185 1104 80-81 0.6945168720297268 1104 82-83 0.5835346501845784 1104 84-85 0.3119936273212751 1104 86-87 0.26678129621251045 1104 88-89 0.1837683431794126 1104 90-91 0.1352753908650257 1104 92-93 -0.03157724417763674 1104 94-95 0.16275296224000257 1104 96-97 -0.03530454686589479 1104 98-99 -0.24106320940530068 1104 100-101 -0.7467703958659158 1105 1 0.05150650081911223 1105 2 0.16450686530502168 1105 3 0.16283898780364936 1105 4 0.26081132201903046 1105 5 0.38816111105115425 1105 6 0.1753617146487585 1105 7 0.3218436847651418 1105 8 -0.09749400939620045 1105 9 0.2389780015800369 1105 10-11 0.20686015059342822 1105 12-13 0.09819380999891791 1105 14-15 0.17663558603246798 1105 16-17 0.24877285804178229 1105 18-19 0.03291249006936425 1105 20-21 -0.38646684881113913 1105 22-23 -0.24306213471781035 1105 24-25 -0.34657898836607615 1105 26-27 -0.06596205864662608 1105 28-29 0.24894913261815788 1105 30-31 0.0033778168796843033 1105 32-33 -0.2251220440788373 1105 34-35 -0.16186089209983123 1105 36-37 -0.21058287552208554 1105 38-39 0.08004636000354282 1105 40-41 -0.2844877921804496 1105 42-43 0.02855024531037742 1105 44-45 0.23915240271711724 1105 46-47 0.18870903496961233 1105 48-49 0.3163305489139461 1105 50-51 0.29632915047034203 1105 52-53 0.1966978912870303 1105 54-55 0.2213600417347692 1105 56-57 0.4943620841169363 1105 58-59 0.4767464563676782 1105 60-61 0.2781729642942139 1105 62-63 0.2644141135803366 1105 64-65 0.46885636517431806 1105 66-67 -0.04867280937802576 1105 68-69 0.002911737866348574 1105 70-71 0.20806018604542587 1105 72-73 -0.08615164174387147 1105 74-75 -0.09491611905553299 1105 76-77 0.11584346525161138 1105 78-79 0.3385490213557816 1105 80-81 0.4998500499726575 1105 82-83 0.5125197416706797 1105 84-85 0.0644074080594379 1105 86-87 0.015335717218903255 1105 88-89 -0.023581473489795002 1105 90-91 0.04917043062404858 1105 92-93 0.2009121997338994 1105 94-95 0.4387913137868864 1105 96-97 0.5766079205245305 1105 98-99 0.3649279871306881 1105 100-101 0.4228711005436665 1106 1 0.06330818909736635 1106 2 0.06567167793684803 1106 3 -0.05599583824226784 1106 4 0.1752998929443308 1106 5 0.33589358805073743 1106 6 0.12991189101918366 1106 7 0.2989313370285984 1106 8 0.3963251217140993 1106 9 0.6004764049873046 1106 10-11 0.4527625303521603 1106 12-13 0.28566134745222627 1106 14-15 0.18564831812556548 1106 16-17 0.20044148939823003 1106 18-19 0.2045010433802048 1106 20-21 0.5084053108571709 1106 22-23 0.3815082305338251 1106 24-25 0.25745811039684696 1106 26-27 0.30010142567424936 1106 28-29 0.022237481330272146 1106 30-31 0.025054894143934803 1106 32-33 -0.1526024084723403 1106 34-35 0.002011172721388732 1106 36-37 -0.01830184810224722 1106 38-39 0.11870106690344784 1106 40-41 -0.18698723890573632 1106 42-43 0.23468685222337626 1106 44-45 -0.04780070217021404 1106 46-47 0.09960470768390906 1106 48-49 -0.37462939399713946 1106 50-51 -0.30306269900280824 1106 52-53 -0.1092065660746826 1106 54-55 -0.1848282794265259 1106 56-57 -0.05128759588703247 1106 58-59 -0.17895633800834077 1106 60-61 -0.14851474939402465 1106 62-63 -0.15097550472786025 1106 64-65 -0.42105551735981805 1106 66-67 -0.45343164935291114 1106 68-69 -0.30290577632684546 1106 70-71 -0.16401253909221225 1106 72-73 -0.0174292772510114 1106 74-75 -0.2381566828989179 1106 76-77 -0.6560910541220366 1106 78-79 -0.38615503313161526 1106 80-81 -0.37930007490934514 1106 82-83 -0.2536900781650111 1106 84-85 -0.2737032830145445 1106 86-87 -0.24002088526789933 1106 88-89 -0.18136751651424277 1106 90-91 0.025644964179598162 1106 92-93 -0.10545971830014267 1106 94-95 -0.2956119850853369 1106 96-97 -0.21378564974587277 1106 98-99 0.07930838720475819 1106 100-101 0.577469603766211 1107 1 0.46468700546409636 1107 2 0.5054379954227493 1107 3 0.46329863753891587 1107 4 0.4544654404418651 1107 5 0.39670923851183204 1107 6 0.44493561740471677 1107 7 0.6060313459819469 1107 8 0.5433391784783552 1107 9 0.725644280356633 1107 10-11 0.8627701407022386 1107 12-13 0.8049222481595422 1107 14-15 0.7409016980163372 1107 16-17 0.7498080396713078 1107 18-19 0.8100163088471248 1107 20-21 0.926414980478441 1107 22-23 0.8217445990538437 1107 24-25 0.7723990182668388 1107 26-27 1.0040811910963967 1107 28-29 0.5123216428487538 1107 30-31 0.8723218831313062 1107 32-33 0.8137106007496158 1107 34-35 0.6749004197443966 1107 36-37 1.1222098360234583 1107 38-39 1.115567393342694 1107 40-41 0.8436444201505751 1107 42-43 1.0766825546139174 1107 44-45 1.0218563844087143 1107 46-47 0.9811854221614809 1107 48-49 1.0280700419416249 1107 50-51 1.0974991238998726 1107 52-53 1.3645936846192015 1107 54-55 1.5051282968112787 1107 56-57 1.274927821786882 1107 58-59 1.4100511828082034 1107 60-61 1.7332674155267398 1107 62-63 1.6088508002233368 1107 64-65 1.7346992503006717 1107 66-67 1.8416149080828426 1107 68-69 1.6976761916201468 1107 70-71 1.697053805253745 1107 72-73 1.8976369775614153 1107 74-75 1.7211391358852168 1107 76-77 1.7878945309795817 1107 78-79 1.9679612709242527 1107 80-81 1.8522336344648132 1107 82-83 1.8628378115296833 1107 84-85 1.7735480376050283 1107 86-87 1.71336572051597 1107 88-89 1.812947104311256 1107 90-91 1.973883391970805 1107 92-93 1.7708609012718846 1107 94-95 1.9128220734695915 1107 96-97 2.404857469602323 1107 98-99 2.540540368581784 1107 100-101 2.8892701230606868 1108 1 -0.10035327421366702 1108 2 0.06572831310020533 1108 3 0.008214278213788617 1108 4 -0.04409057111003989 1108 5 0.2524699924258016 1108 6 0.10295355326123001 1108 7 0.17715157702010487 1108 8 -0.07645106319970552 1108 9 0.3317850312306234 1108 10-11 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2316 76-77 -2.6690697862788504 2316 78-79 -3.047782413937078 2316 80-81 -3.0508311950706073 2316 82-83 -2.887118026661451 2316 84-85 -3.158663585783067 2316 86-87 -3.198421066270818 2316 88-89 -3.7127550684497415 2316 90-91 -3.8634907468739215 2316 92-93 -4.0612579823175885 2316 94-95 -4.036171037288227 2316 96-97 -4.292347087896353 2316 98-99 -4.586689802766035 2316 100-101 -5.555660101281303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 845.0 3 317.0 4 250.0 5 282.0 6 351.0 7 417.0 8 517.0 9 703.0 10 1018.0 11 1648.0 12 2550.0 13 3531.0 14 4147.0 15 5506.0 16 7264.0 17 9864.0 18 8446.0 19 5417.0 20 5669.0 21 6296.0 22 7191.0 23 7976.0 24 8604.0 25 9531.0 26 10578.0 27 12386.0 28 14270.0 29 17115.0 30 20203.0 31 24744.0 32 30638.0 33 38288.0 34 49785.0 35 67986.0 36 83691.0 37 94749.0 38 69854.0 39 10152.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.14408280248831 19.392985065801962 14.479543665180197 15.983388466529533 2 13.325056321023613 26.920982537371025 33.84006696289658 25.913894178708784 3 28.9973565924158 26.858670267965913 30.53392476790446 13.610048371713829 4 10.079198706426316 16.402800667952146 45.23835091143098 28.27964971419056 5 8.944077723699221 43.69358782594349 34.87278223013599 12.489552220221297 6 23.548100377169344 40.319761152024306 16.15072452939446 19.981413941411887 7 22.18867877550512 37.73544387316842 22.34429270357414 17.731584647752314 8 22.854221843226473 24.098787179016362 21.05786497446447 31.9891260032927 9 20.87619361775291 24.497540801227345 27.70562433774345 26.920641243276293 10-11 24.675938139339788 24.149641600072215 26.278095931804796 24.896324328783198 12-13 32.65981396702517 24.911656515239592 24.14226472151472 18.28626479622051 14-15 24.94859268837483 26.393132309932987 33.380792221099284 15.277482780592894 16-17 17.05863267959414 30.443960154768675 31.75146447486254 20.745942690774644 18-19 15.86735678404472 35.449878306120794 30.580333522150777 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19.72936013180392 26.46509732051595 28.562338492749383 25.243204054930747 46-47 24.061829558767016 30.426577593435876 28.765711369749848 16.745881478047256 48-49 21.429421533890032 28.742216396809162 29.67893244551964 20.149429623781167 50-51 14.282236039146165 30.575454225108338 36.25594205085641 18.886367684889084 52-53 18.833777241216225 34.57005239772487 26.039553436967154 20.556616924091752 54-55 15.832241051391055 28.07733745094455 30.547515236846916 25.54290626081748 56-57 21.884490204863962 26.827020013441867 27.77969656236776 23.508793219326414 58-59 26.707837304674133 26.70425905297679 26.528457991325073 20.059445651024003 60-61 20.878109644302043 28.631947085971294 33.99180277498392 16.498140494742746 62-63 18.599037762194246 29.877289398659713 31.868195979137255 19.65547686000879 64-65 17.277623754982404 35.41536369604924 28.730929955411177 18.57608259355718 66-67 20.14443836826961 28.469558493727494 25.804745276465525 25.58125786153737 68-69 17.21708405971626 29.694923059808836 29.2467632687893 23.841229611685606 70-71 24.03000366410646 28.746587752237946 26.242237112030637 20.981171471624958 72-73 21.075452837600785 24.29768845706551 34.35692587737893 20.269932827954772 74-75 16.866871928271493 35.47125896723023 28.3775205870959 19.28434851740238 76-77 18.919541231307292 27.63946020623432 28.40988294303975 25.031115619418635 78-79 19.504292485019292 28.075723279640634 31.047976388803512 21.37200784653656 80-81 23.550386643958447 27.568076023250764 28.238585658811893 20.64295167397889 82-83 20.06708684105304 25.692766538726623 34.06430815139347 20.175838468826868 84-85 18.872203145639467 29.130460488135785 31.14312208373332 20.85421428249143 86-87 18.89785830807038 30.90085485643873 29.837749196991936 20.363537638498958 88-89 18.049855151833864 29.76979876840163 26.115766512846523 26.064579566917985 90-91 19.642279231003815 24.723202449634982 29.42370407712367 26.210814242237536 92-93 23.41280306831752 29.467128781425167 29.73840726002513 17.381660890232187 94-95 20.839397803021875 27.362031531871427 30.155667903286908 21.642902761819784 96-97 14.140824823105502 28.319611174380327 35.64229661022223 21.897267392291944 98-99 19.115497161334392 31.430023022095455 26.59756147848691 22.856918338083247 100-101 17.17944467919372 26.29443473023418 30.50373562614635 26.02238496442576 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 869.0 1 668.5 2 742.0 3 994.5 4 825.5 5 799.0 6 1280.5 7 2374.0 8 3469.0 9 3730.0 10 3028.0 11 2167.5 12 1883.0 13 1845.0 14 1767.0 15 1574.5 16 1443.0 17 1405.5 18 1362.5 19 1323.5 20 1331.0 21 1395.5 22 1509.5 23 1680.5 24 1826.5 25 2261.5 26 3176.5 27 3937.0 28 4180.0 29 4814.5 30 6144.5 31 7433.0 32 9648.0 33 12874.5 34 16062.0 35 17973.5 36 20319.0 37 22312.0 38 20752.0 39 18371.0 40 15973.0 41 14093.5 42 12279.0 43 11266.0 44 11303.0 45 11381.0 46 15388.0 47 46768.0 48 117012.0 49 110707.5 50 39421.0 51 11029.5 52 3848.5 53 2044.0 54 1429.5 55 1112.0 56 927.0 57 799.0 58 705.0 59 583.5 60 494.5 61 460.5 62 408.5 63 373.5 64 316.0 65 291.0 66 273.5 67 187.0 68 116.5 69 93.0 70 89.0 71 74.5 72 57.0 73 77.5 74 88.0 75 54.0 76 33.5 77 29.0 78 19.5 79 14.5 80 11.0 81 7.5 82 7.0 83 3.5 84 2.0 85 3.0 86 2.5 87 2.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01477952833080007 2 0.005756237349890554 3 0.007778699121473721 4 0.034226276134484375 5 0.04667219472884233 6 0.056628929604328686 7 0.02566970710086328 8 0.022869375417132742 9 0.014312806383511647 10-11 0.04239391021203178 12-13 0.04052702242287809 14-15 0.018591090900322193 16-17 0.022480440461059056 18-19 0.028781186749452767 20-21 0.022791588425918004 22-23 0.014390593374726384 24-25 0.0033448406222337 26-27 0.002566970710086328 28-29 0.0013223788506505326 30-31 0.0066896812444674 32-33 0.0012445918594357953 34-35 0.0015557398242947442 36-37 0.00528951540260213 38-39 0.004667219472884233 40-41 0.003422627613448437 42-43 8.556569033621093E-4 44-45 0.0012445918594357953 46-47 0.002178035754012642 48-49 5.445089385031605E-4 50-51 0.0016335268155094815 52-53 7.778699121473721E-4 54-55 0.002100248762797905 56-57 0.0020224617715831677 58-59 0.0014001658418652699 60-61 3.8893495607368605E-4 62-63 0.0013223788506505326 64-65 0.0029559056661600143 66-67 0.0024113967276568537 68-69 0.006456320270823188 70-71 0.009256651954553729 72-73 0.0044338584992400214 74-75 0.003733775578307386 76-77 0.002644757701301065 78-79 0.03181487940682752 80-81 0.021080274619193783 82-83 0.004978367437743181 84-85 0.007778699121473721 86-87 0.006456320270823188 88-89 0.006067385314749502 90-91 0.011356900717351633 92-93 0.012990427532861113 94-95 0.005367302393816867 96-97 0.0038893495607368604 98-99 0.021391422584052733 100-101 0.03912685658101282 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 642781.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.612852034110263 #Duplication Level Percentage of deduplicated Percentage of total 1 74.64799293248913 11.654680682982617 2 9.096999138715756 2.840602030143952 3 3.8000090332779775 1.7798693629455433 4 2.215999864239769 1.383923119519358 5 1.498999858754934 1.1701831496946484 6 1.083999897375846 1.0154598001631876 7 0.8429999201866566 0.9213143113048723 8 0.6879999348616519 0.8593312945977977 9 0.5719999458442797 0.803749546618724 >10 4.49399957451782 14.605353309545798 >50 0.5999999431933004 6.434055714094332 >100 0.3899999630756452 11.131650189359322 >500 0.022999997822409846 2.495558032858136 >1k 0.02799999734902068 10.44327960834884 >5k 0.01099999895854384 11.822987211975427 >10k+ 0.00699999933725517 20.638002635847446 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 38065 5.921923641177944 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 25715 4.000584958173935 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 19649 3.0568731807567433 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 15205 2.365502402840159 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11785 1.833439382931356 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 11534 1.7943903133415582 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 10233 1.5919885622008116 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9904 1.5408047219815146 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9680 1.5059561499173124 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7943 1.2357241424373155 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7715 1.2002532744433951 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6809 1.0593032463622913 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6343 0.9868057705501563 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5931 0.9227092897892128 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5614 0.8733923373590694 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5400 0.8400995051191619 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5236 0.8145853720007281 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5151 0.8013615834942228 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4808 0.747999707520913 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4760 0.7405321563642983 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4348 0.6764356756033548 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4097 0.6373866060135567 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4038 0.6282077410502177 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3637 0.5658225740959985 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3543 0.551198619747628 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3304 0.5140164379469835 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3247 0.5051487209485035 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3230 0.5025039632472024 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2708 0.42129434441901675 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1984 0.3086587811400773 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1976 0.30741418928064146 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1930 0.30025778608888565 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1927 0.29979106414159723 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1801 0.2801887423554834 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1620 0.2520298515357486 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1567 0.24378443046698642 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1562 0.24300656055483905 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1457 0.22667129239974426 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1366 0.21251405999866205 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1309 0.20364634300018203 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1302 0.2025573251231757 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1137 0.17688761802231243 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1118 0.1739317123561524 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1077 0.16755317907654396 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1023 0.15915218402535233 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1013 0.1575964442010576 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 986 0.1533959466754618 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 888 0.1381496963973733 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 880 0.1369051045379375 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 855 0.13301575497720064 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 839 0.13052657125832903 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 802 0.12477033390843849 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 776 0.12072541036527215 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 732 0.11388015513837527 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 726 0.11294671124379843 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 711 0.1106131015073563 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 692 0.1076571958411963 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 663 0.10314555035074154 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 660 0.10267882840345312 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 649 0.10096751459672891 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.3336097364421162E-4 0.0 14-15 0.0 0.0 0.0 3.8893495607368605E-4 0.0 16-17 0.0 0.0 0.0 4.667219472884233E-4 0.0 18-19 0.0 0.0 0.0 4.667219472884233E-4 0.0 20-21 0.0 0.0 0.0 7.000829209326349E-4 0.0 22-23 0.0 0.0 0.0 8.556569033621093E-4 0.0 24-25 0.0 0.0 0.0 0.0012445918594357953 0.0 26-27 0.0 0.0 0.0 0.0018668877891536931 0.0 28-29 0.0 0.0 0.0 0.0036559885870926486 0.0 30-31 0.0 0.0 0.0 0.0071564031917558234 0.0 32-33 0.0 0.0 0.0 0.011979196647069531 0.0 34-35 0.0 0.0 0.0 0.016179694172665342 0.0 36-37 0.0 0.0 0.0 0.019757895768543254 0.0 38-39 0.0 0.0 0.0 0.02512519816236012 0.0 40-41 0.0 0.0 0.0 0.03321504524869279 0.0 42-43 0.0 0.0 0.0 0.04231612322081704 0.0 44-45 0.0 0.0 0.0 0.06674123846224453 0.0 46-47 0.0 0.0 0.0 0.12329238107535849 0.0 48-49 0.0 0.0 0.0 0.15954111898142603 0.0 50-51 0.0 0.0 0.0 0.20574659176297994 0.0 52-53 0.0 0.0 0.0 0.25420788728976124 0.0 54-55 0.0 0.0 0.0 0.3086587811400773 0.0 56-57 0.0 0.0 0.0 0.3805339610224945 0.0 58-59 0.0 0.0 0.0 0.4797901618124991 0.0 60-61 0.0 0.0 0.0 0.5953816307575986 0.0 62-63 0.0 0.0 0.0 0.7323645222867509 0.0 64-65 0.0 0.0 0.0 0.8195637394384713 0.0 66-67 0.0 0.0 0.0 0.9907729071021079 0.0 68-69 0.0 0.0 0.0 1.1983863866542415 0.0 70-71 0.0 0.0 0.0 1.3310909936665833 0.0 72-73 0.0 0.0 0.0 1.5322481529478935 0.0 74-75 0.0 0.0 0.0 1.7166033221268209 0.0 76-77 0.0 0.0 0.0 1.8312613471773433 0.0 78-79 0.0 0.0 0.0 2.0496094315171107 0.0 80-81 0.0 0.0 0.0 2.2674907939095896 0.0 82-83 0.0 0.0 0.0 2.488483635950658 0.0 84-85 0.0 0.0 0.0 2.862094554755041 0.0 86-87 0.0 0.0 0.0 3.3407179116993193 0.0 88-89 0.0 0.0 0.0 3.9213978011173323 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGTATT 35 2.7790415E-4 54.317215 1 GTTAGAG 40 5.3710124E-4 47.52756 3 GTATGGT 65 2.5772752E-6 43.871593 1 GTTATAT 65 2.5772752E-6 43.871593 1 ATGACTC 85 3.6297024E-7 39.12816 8 AGGTTAG 50 0.0016106883 38.022053 1 CATTCCG 125 1.3460522E-10 38.010212 9 GGACGAC 25 0.0016149897 38.0043 20-21 CATATAG 160 1.8189894E-12 35.64567 3 GCTTATG 200 0.0 35.64567 1 CTTATGT 175 0.0 35.30619 2 ACATATA 175 0.0 35.30619 2 CATGACT 95 8.673742E-7 35.009403 7 ATGAGAG 55 0.0025714566 34.5655 5 GACATAT 180 0.0 34.325462 1 GGATATG 235 0.0 32.359188 1 GGATGAC 60 0.0039444743 31.675179 8 GGTGATG 60 0.0039444743 31.675179 8 TATGTAC 200 0.0 30.892918 4 TAGGCAT 140 1.693843E-8 30.553432 5 >>END_MODULE