##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936650_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 642781 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.392755853082154 31.0 31.0 34.0 30.0 34.0 2 31.49973163488031 31.0 31.0 34.0 30.0 34.0 3 31.78915680457263 31.0 31.0 34.0 30.0 34.0 4 35.48764198070572 37.0 35.0 37.0 33.0 37.0 5 35.35683382053919 37.0 35.0 37.0 33.0 37.0 6 35.596372637025674 37.0 35.0 37.0 33.0 37.0 7 35.542556485023674 37.0 35.0 37.0 33.0 37.0 8 35.58937958651547 37.0 35.0 37.0 33.0 37.0 9 37.10056613372206 39.0 37.0 39.0 33.0 39.0 10-11 36.974179386136186 39.0 37.0 39.0 33.0 39.0 12-13 36.9180786302022 39.0 37.0 39.0 33.0 39.0 14-15 37.641221504680445 39.0 36.5 41.0 32.0 41.0 16-17 37.75680052770694 39.0 37.0 41.0 32.5 41.0 18-19 38.054835161586915 40.0 37.0 41.0 33.0 41.0 20-21 38.19235322761563 40.0 37.0 41.0 33.5 41.0 22-23 38.097570556690386 40.0 37.0 41.0 33.0 41.0 24-25 37.86618848410267 39.5 37.0 41.0 32.5 41.0 26-27 37.531450836287945 39.0 36.5 41.0 32.5 41.0 28-29 37.51910370717243 39.0 36.0 41.0 32.5 41.0 30-31 37.610411633200115 39.0 36.0 41.0 33.0 41.0 32-33 37.35132494582136 39.0 35.5 41.0 32.5 41.0 34-35 37.004055035852026 39.0 35.0 41.0 31.0 41.0 36-37 36.66489830906639 38.5 35.0 40.5 30.5 41.0 38-39 36.452649502707764 38.0 35.0 40.0 30.0 41.0 40-41 36.46221107966788 39.0 35.0 40.0 30.5 41.0 42-43 36.16032210037322 38.0 35.0 40.0 30.0 41.0 44-45 35.76169566306409 38.0 35.0 40.0 29.5 41.0 46-47 35.257784533145816 38.0 35.0 40.0 27.0 41.0 48-49 34.671291621874325 38.0 34.0 40.0 24.0 41.0 50-51 34.45465562921119 38.0 34.0 40.0 23.5 41.0 52-53 34.36842641583992 38.0 34.0 40.0 23.0 41.0 54-55 34.24973824677456 38.0 33.5 40.0 23.0 41.0 56-57 33.82000245806893 38.0 33.0 40.0 22.5 41.0 58-59 33.50601993525011 38.0 33.0 40.0 21.5 41.0 60-61 32.79828744160142 36.5 31.5 39.0 18.5 40.5 62-63 32.69202730012243 36.0 31.5 39.0 19.0 40.0 64-65 32.29002179591494 36.0 31.0 39.0 16.0 40.0 66-67 31.660213976455434 35.5 30.5 38.5 12.5 40.0 68-69 31.053114513341246 35.0 30.0 37.0 8.0 39.0 70-71 30.289595523203083 34.0 29.0 37.0 6.5 39.0 72-73 29.78077058904977 34.0 29.0 36.0 2.0 38.5 74-75 29.473993475227175 34.0 29.0 36.0 2.0 37.0 76-77 27.988549754893192 31.5 27.0 34.0 2.0 36.0 78-79 28.795436548373395 34.0 29.0 35.0 2.0 36.0 80-81 28.41085999741747 33.5 28.0 35.0 2.0 36.0 82-83 28.004103263786575 33.0 27.0 35.0 2.0 36.0 84-85 27.49928435968082 33.0 26.0 35.0 2.0 35.0 86-87 27.415754354904703 33.0 26.0 35.0 2.0 35.0 88-89 26.92540616477463 33.0 25.5 35.0 2.0 35.0 90-91 26.252804609968248 32.0 25.0 34.0 2.0 35.0 92-93 25.85551610890801 32.0 24.0 34.0 2.0 35.0 94-95 25.224623160921062 32.0 20.0 34.0 2.0 35.0 96-97 24.42972178704722 31.0 16.5 34.0 2.0 35.0 98-99 23.76169877454374 31.0 2.0 34.0 2.0 35.0 100-101 21.102146765383544 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.47461665423136523 1101 2 0.44349993638052254 1101 3 0.3321519390494778 1101 4 0.13130026930422645 1101 5 0.21261367478538773 1101 6 0.21938831860144603 1101 7 0.1597337021632299 1101 8 0.22519859889692384 1101 9 0.277178314954881 1101 10-11 0.24006815383098257 1101 12-13 0.05018765598588715 1101 14-15 0.244326303683998 1101 16-17 0.47855834373819306 1101 18-19 0.5242395773812376 1101 20-21 0.31287701356784225 1101 22-23 0.2623822364117885 1101 24-25 0.25457627101868496 1101 26-27 0.12873728107312843 1101 28-29 0.1714402275691853 1101 30-31 0.16129004692961502 1101 32-33 0.19773713408542193 1101 34-35 0.02315068213233218 1101 36-37 0.20385796557923186 1101 38-39 0.43166984695779576 1101 40-41 0.4951961697102689 1101 42-43 0.5998381159715294 1101 44-45 0.8083535324898818 1101 46-47 0.7941759004517124 1101 48-49 0.7343820323506378 1101 50-51 0.6404023685630307 1101 52-53 0.5182124278090399 1101 54-55 0.708938685343405 1101 56-57 0.6767522010174858 1101 58-59 0.6437843707854896 1101 60-61 0.703421345184374 1101 62-63 0.6629366609788789 1101 64-65 1.0969515980978315 1101 66-67 0.4923956723524192 1101 68-69 0.546867595987969 1101 70-71 0.6126600081208444 1101 72-73 0.667197502698599 1101 74-75 0.7499727711566173 1101 76-77 0.9498461468814874 1101 78-79 0.9896079387546841 1101 80-81 0.9652004269317374 1101 82-83 0.7117414296623572 1101 84-85 0.9455594362016306 1101 86-87 0.9367417981991011 1101 88-89 0.8667785732996478 1101 90-91 0.6316953748005432 1101 92-93 0.6917064956335714 1101 94-95 0.6488172718800165 1101 96-97 0.6173199085103853 1101 98-99 0.7186617314184041 1101 100-101 0.8288026183203634 1102 1 0.49964900505811016 1102 2 0.5381320954986641 1102 3 0.25677211637993125 1102 4 0.20967074617937698 1102 5 0.11023169539406297 1102 6 0.15084290762612795 1102 7 0.011010960730210684 1102 8 0.10740324783053978 1102 9 -0.01074883577600616 1102 10-11 0.1519325199944177 1102 12-13 0.2136288254083638 1102 14-15 0.3187260161210972 1102 16-17 0.3515681688040928 1102 18-19 0.17956907654251353 1102 20-21 0.1672048352788451 1102 22-23 0.19221449138422741 1102 24-25 -0.009418456994737312 1102 26-27 -0.06427494035030179 1102 28-29 -0.1704361203819289 1102 30-31 -0.2107564424065984 1102 32-33 -0.04780803725414273 1102 34-35 -0.010597221054815975 1102 36-37 -0.17357793185666281 1102 38-39 -0.0638130190858206 1102 40-41 0.10840728880923223 1102 42-43 0.3123590840999739 1102 44-45 0.16541559815007645 1102 46-47 0.20466955010424215 1102 48-49 0.2188416537261446 1102 50-51 0.3248595935809959 1102 52-53 0.30370008656772285 1102 54-55 0.45904412507501036 1102 56-57 0.3161483189182732 1102 58-59 0.2687735871765824 1102 60-61 0.39203864688578705 1102 62-63 0.3645781658914089 1102 64-65 0.34538198391139474 1102 66-67 0.3124220322853155 1102 68-69 0.275547202985333 1102 70-71 0.18999525854020405 1102 72-73 0.26494732297178203 1102 74-75 -0.13652615048249217 1102 76-77 0.07765347973554881 1102 78-79 0.4454862037918268 1102 80-81 0.3359672613434306 1102 82-83 0.44939539563408104 1102 84-85 0.3550202557559068 1102 86-87 0.2862577339767185 1102 88-89 0.21006158312471257 1102 90-91 0.14415403763304013 1102 92-93 0.39281600917299286 1102 94-95 -0.08517505977586737 1102 96-97 0.11600670458036078 1102 98-99 -0.09864476271051714 1102 100-101 -0.1887746642101895 1103 1 0.4205042223483737 1103 2 0.41974957167807503 1103 3 0.3661516190524843 1103 4 0.2798682827817487 1103 5 0.06099627824323761 1103 6 0.07227911492336148 1103 7 0.198745099703018 1103 8 0.18076137007084014 1103 9 0.05228160810035831 1103 10-11 0.1170010768152423 1103 12-13 0.20893675011205914 1103 14-15 0.3215914588354778 1103 16-17 0.36056886599210713 1103 18-19 0.16647588574539185 1103 20-21 0.22380255779801672 1103 22-23 -0.0696005213975539 1103 24-25 -0.03355278188435307 1103 26-27 0.13229489464853827 1103 28-29 0.24802774203963196 1103 30-31 0.26653846812094883 1103 32-33 0.16448803525341305 1103 34-35 0.23222871433909376 1103 36-37 0.27888314181287654 1103 38-39 0.6160963464730926 1103 40-41 0.48646213426589213 1103 42-43 0.48518037157382565 1103 44-45 0.41896660907269023 1103 46-47 0.2523362942362368 1103 48-49 0.5183652419204279 1103 50-51 0.47007455988357805 1103 52-53 0.4153686898677478 1103 54-55 0.38366880553050464 1103 56-57 0.2319163877074928 1103 58-59 0.5110162086034862 1103 60-61 0.5274441779106596 1103 62-63 0.09861256049982359 1103 64-65 0.20979053608429865 1103 66-67 0.38105554378892137 1103 68-69 0.40352070984101474 1103 70-71 0.4830071107363487 1103 72-73 0.3720214572966718 1103 74-75 0.5814520042932472 1103 76-77 0.48033997752778745 1103 78-79 0.6086444654697267 1103 80-81 0.6634573845133147 1103 82-83 0.5870249564056316 1103 84-85 0.7693869766483097 1103 86-87 0.7437106737861541 1103 88-89 0.6006681366919935 1103 90-91 0.48591792329420613 1103 92-93 0.41912788460873784 1103 94-95 0.4872046752926913 1103 96-97 0.3535223913105483 1103 98-99 0.24793204416840453 1103 100-101 0.14630551241077328 1104 1 0.14104802899489854 1104 2 0.08591271367203035 1104 3 -0.08097829031307313 1104 4 -0.33795649063215194 1104 5 -0.28643574592594234 1104 6 -0.12862722948751326 1104 7 -0.171345534738073 1104 8 -0.18630811633399702 1104 9 -0.3117667302682179 1104 10-11 -0.10731916487660698 1104 12-13 -0.29559497194232165 1104 14-15 -0.182940263218903 1104 16-17 -0.26027705545804736 1104 18-19 -0.2005936006594382 1104 20-21 -0.1968923062503194 1104 22-23 -0.24331653348213678 1104 24-25 -0.11361320484508042 1104 26-27 -0.0897594860161206 1104 28-29 -0.24895110992410707 1104 30-31 -0.3573286013654595 1104 32-33 -0.5688956505471907 1104 34-35 -0.512484276597462 1104 36-37 -0.5489114201206675 1104 38-39 -0.6346589405359779 1104 40-41 -0.650244814374588 1104 42-43 -0.5155749154352307 1104 44-45 -0.5107916990844075 1104 46-47 -0.2861833432259928 1104 48-49 -0.397042613064464 1104 50-51 -0.6756958328656708 1104 52-53 -0.7175618237274222 1104 54-55 -0.7560592911462294 1104 56-57 -0.6683857270961298 1104 58-59 -0.4965366614871414 1104 60-61 -0.7724047646875292 1104 62-63 -0.890058134364228 1104 64-65 -1.0386988898975744 1104 66-67 -0.8198508006219569 1104 68-69 -0.525482311307023 1104 70-71 -0.6907089013482484 1104 72-73 -0.9512413825824808 1104 74-75 -1.086976998727902 1104 76-77 -0.6367295088770462 1104 78-79 -0.67987517199251 1104 80-81 -0.6990803798371381 1104 82-83 -0.7173557082469308 1104 84-85 -0.49345290250576923 1104 86-87 -0.27517150144043256 1104 88-89 -0.3328666065104251 1104 90-91 -0.577526185467125 1104 92-93 -0.32558510632782145 1104 94-95 -0.3466321827133534 1104 96-97 -0.6260848594447381 1104 98-99 -0.2596208259222301 1104 100-101 0.12515747615699624 1105 1 0.2655426366490694 1105 2 0.4405972860944196 1105 3 0.24166597622928165 1105 4 0.25557807327364657 1105 5 0.0417039619998647 1105 6 0.10482145133761378 1105 7 0.23291007052249313 1105 8 0.04379220012453544 1105 9 0.3029786237081282 1105 10-11 0.15378826069288465 1105 12-13 0.041948843669032954 1105 14-15 0.10106109943540531 1105 16-17 0.12370010215264671 1105 18-19 0.03738007859683279 1105 20-21 0.17230184509395485 1105 22-23 -0.009036364584723344 1105 24-25 -0.004898940902521076 1105 26-27 0.046642658371446544 1105 28-29 0.1812644046123637 1105 30-31 0.30473088925284486 1105 32-33 0.21714745236010202 1105 34-35 0.14593325144004865 1105 36-37 0.3279924858561927 1105 38-39 0.09820478506848218 1105 40-41 0.4207880238012507 1105 42-43 0.5153492689892616 1105 44-45 0.5284742605559245 1105 46-47 0.4711543709214041 1105 48-49 0.448914696410462 1105 50-51 0.6175370644780571 1105 52-53 0.49709657138853913 1105 54-55 0.5246273252576188 1105 56-57 0.3940072552341789 1105 58-59 0.4879564221457642 1105 60-61 0.3939331915103068 1105 62-63 0.46674662058212135 1105 64-65 0.4410450745299741 1105 66-67 0.6000641231321602 1105 68-69 0.5261954568086615 1105 70-71 0.3108375319937515 1105 72-73 0.41371016250613835 1105 74-75 0.3725288597891385 1105 76-77 0.21586849913751038 1105 78-79 0.7363193469098412 1105 80-81 0.40881136086408887 1105 82-83 0.4882997553693009 1105 84-85 0.5725596303279232 1105 86-87 0.15965193621364904 1105 88-89 0.37128276550316386 1105 90-91 0.523969148338356 1105 92-93 1.0587461621061145 1105 94-95 1.166057535522011 1105 96-97 0.7554292108574678 1105 98-99 0.6355803000605356 1105 100-101 0.8752404008434489 1106 1 0.22405832480989574 1106 2 0.4464800641206068 1106 3 0.1314378463881276 1106 4 0.14578127187894552 1106 5 0.13418439414128613 1106 6 0.0682624135387826 1106 7 -0.09236803470358268 1106 8 0.11208888183124088 1106 9 -0.017968130940502647 1106 10-11 -0.029114195846823065 1106 12-13 0.20307034279508684 1106 14-15 0.3708288143882754 1106 16-17 0.28170632592855327 1106 18-19 0.19394327160163272 1106 20-21 -0.07654531107404239 1106 22-23 0.0921700001068686 1106 24-25 -0.23637665861047452 1106 26-27 0.21666171812059787 1106 28-29 0.14598467343612498 1106 30-31 -0.007460575412096659 1106 32-33 -0.11372888961335548 1106 34-35 -0.31066629961704706 1106 36-37 -0.016461669123962963 1106 38-39 -0.01990129914670291 1106 40-41 0.05176169695816668 1106 42-43 0.06722385403323727 1106 44-45 -0.0024616995902349004 1106 46-47 -0.24581399983136265 1106 48-49 -0.2842668815741689 1106 50-51 -0.24478447620005284 1106 52-53 0.062448476685979415 1106 54-55 -0.32739540191246164 1106 56-57 -0.19118688773200176 1106 58-59 -0.36275199530791014 1106 60-61 0.07138532760233574 1106 62-63 -0.799676605212678 1106 64-65 -0.19543876307373864 1106 66-67 -0.4532365557020377 1106 68-69 -0.08257948007285876 1106 70-71 -0.04867585725419943 1106 72-73 -0.2634416244582596 1106 74-75 -0.04480738218837743 1106 76-77 -0.014932868467237626 1106 78-79 -0.11164527964102433 1106 80-81 -0.1718854491120183 1106 82-83 -0.18673152158872952 1106 84-85 0.1164580265087487 1106 86-87 0.12134613111991044 1106 88-89 0.002434841525367659 1106 90-91 -0.03098119243369979 1106 92-93 -0.24887231772081364 1106 94-95 -0.2335885944907723 1106 96-97 -0.5502607114130846 1106 98-99 -0.37261268215788945 1106 100-101 -0.14586985212729786 1107 1 0.5648677079238702 1107 2 0.52043476015427 1107 3 0.38074396152824974 1107 4 0.19824792063890584 1107 5 0.12447000610296044 1107 6 0.3412501474451304 1107 7 0.1291155358936038 1107 8 0.07589495220459952 1107 9 0.14780320042309114 1107 10-11 0.30903156474052196 1107 12-13 0.562625808817117 1107 14-15 0.5838290829888635 1107 16-17 0.6324203558327497 1107 18-19 0.6770326260648858 1107 20-21 0.38264978248849957 1107 22-23 0.39417778952400084 1107 24-25 0.5112954696015493 1107 26-27 0.5404485387849434 1107 28-29 0.34374185850193584 1107 30-31 0.4696859909770197 1107 32-33 0.5404926163406287 1107 34-35 0.6009864893524224 1107 36-37 0.6834331289779314 1107 38-39 0.8178863273194068 1107 40-41 0.9987466553250712 1107 42-43 0.8242746195449158 1107 44-45 0.8384372170540502 1107 46-47 0.8579777451771449 1107 48-49 0.9149452233591262 1107 50-51 0.7688917705543474 1107 52-53 0.8211108457431848 1107 54-55 0.9356429184385107 1107 56-57 0.9814449503913778 1107 58-59 0.5777529738030225 1107 60-61 0.45096272933928816 1107 62-63 0.5189396540764264 1107 64-65 0.29335835394658716 1107 66-67 0.3135733646131236 1107 68-69 0.4416281481839235 1107 70-71 0.28503799358293946 1107 72-73 0.39654360250924015 1107 74-75 0.7840268912470201 1107 76-77 0.816218085064861 1107 78-79 0.8050437704082185 1107 80-81 0.8404142196139226 1107 82-83 0.6669594981981852 1107 84-85 0.755917691653341 1107 86-87 1.118816808888738 1107 88-89 1.1539479581875582 1107 90-91 1.1484551939930192 1107 92-93 0.9129819216918484 1107 94-95 1.1724795405257922 1107 96-97 1.3741841806990145 1107 98-99 1.5616920899792746 1107 100-101 1.5595536414710587 1108 1 0.3978362441755827 1108 2 0.42209154689997774 1108 3 0.15482816885458917 1108 4 0.1872736084334008 1108 5 0.0859383143564969 1108 6 -0.024732524693490632 1108 7 0.018452821195324987 1108 8 0.050837952660593544 1108 9 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-1.8137022351285772 2316 98-99 -2.4070855111512692 2316 100-101 -3.3405302659657927 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 4.0 5 21.0 6 67.0 7 123.0 8 286.0 9 559.0 10 930.0 11 1526.0 12 2210.0 13 3269.0 14 4187.0 15 5724.0 16 7626.0 17 9614.0 18 7677.0 19 5036.0 20 4776.0 21 5095.0 22 5672.0 23 6341.0 24 7447.0 25 8344.0 26 9738.0 27 11806.0 28 14452.0 29 17662.0 30 21309.0 31 25916.0 32 31601.0 33 39355.0 34 51016.0 35 69100.0 36 94337.0 37 109567.0 38 57451.0 39 2933.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.14724248357181 20.546946446136584 15.062397274695712 15.243413795595892 2 13.330937221879921 29.047716848086537 33.10725866178731 24.514087268246232 3 30.534889148191365 26.529599377674057 29.76009334889148 13.175418125243096 4 10.491159991473511 16.98840677575801 44.14776343038697 28.372669802381505 5 8.853404192096532 44.72036354528214 34.384339300632725 12.041892961988609 6 24.314813287884988 41.563145145858385 15.411003125481306 18.71103844077532 7 23.072088316238347 37.81178970753647 21.46843170535532 17.647690270869862 8 24.108988909130794 24.590490384750016 20.457823115493458 30.842697590625733 9 20.97492368823103 25.514268200473584 26.410250825349507 27.100557285945875 10-11 24.78435417488295 25.10709365667956 26.293661035644472 23.814891132793022 12-13 33.352676380195106 25.218873316785434 23.49456774124293 17.93388256177653 14-15 24.433516236478674 27.463630692257553 33.16448370440321 14.938369366860563 16-17 17.172489541539033 30.129390881186595 31.965086086863177 20.7330334904112 18-19 16.05920212327371 35.84338989484755 30.62458286725961 17.47282511461913 20-21 21.17579704440548 26.6278094716552 28.02221907617058 24.174174407768742 22-23 18.106526274520785 27.823050510990694 30.13561595031512 23.9348072641734 24-25 24.491545331924872 28.161224429471314 28.807089817527277 18.54014042107654 26-27 22.66495120422041 22.616412121702414 37.51285430029824 17.205782373778938 28-29 16.709501369828917 34.38729520629888 31.987722101306666 16.91548132256554 30-31 19.03929954370151 27.95151070115638 30.104110109041805 22.905079646100305 32-33 19.744462379183602 29.151902919040857 31.679897318216295 19.42373738355925 34-35 24.388513608119755 26.16782154958633 30.649151028815545 18.79451381347837 36-37 21.77021563918922 24.365960419345527 36.0915795623118 17.772244379153452 38-39 19.421311457557085 29.28758006226071 34.42968911651091 16.8614193636713 40-41 18.83179518454674 31.104806740887792 31.78795016522394 18.27544790934153 42-43 16.533975809136763 32.9483042435143 26.89327578642088 23.624444160928054 44-45 20.60351385956697 25.790525056435516 28.76904455566463 24.836916528332882 46-47 24.459352132681882 31.00453427778655 28.397927409549755 16.138186179981815 48-49 22.354990357454867 29.103605043840712 29.243478348810413 19.297926249894008 50-51 14.693996930518164 30.67428254945903 35.87591079055881 18.755809729463994 52-53 19.783842482248758 35.1000043561325 25.63809562333145 19.478057538287295 54-55 16.479158336276523 28.663647240585817 29.59289091636107 25.264303506776592 56-57 22.109289370940854 27.21787163668017 27.964493828581833 22.708345163797148 58-59 27.79884096301194 26.718136206293025 25.76570339543386 19.71731943526117 60-61 20.757802174550584 29.446591889425726 33.60977419804015 16.185831737983534 62-63 19.06197662759338 29.466221403884614 31.81629720901758 19.655504759504424 64-65 17.677208143666363 35.92826017783248 28.28732961092046 18.1072020675807 66-67 20.846093948726246 28.23907008076818 25.94188704900932 24.972948921496254 68-69 17.909379640483806 30.133614818744505 28.24611200868726 23.710893532084437 70-71 24.21136806142745 29.713375375034325 26.010677150384776 20.064579413153446 72-73 21.9940384049933 24.286576610074036 33.80272596732013 19.91665901761253 74-75 17.38920409906329 36.36798536359973 27.74140803788538 18.5014024994516 76-77 19.779688941508123 27.981184648390645 27.60632447224759 24.632801937853642 78-79 19.71001549654284 28.922523509313486 29.9936675773738 21.373793416769875 80-81 23.756009895906267 28.159921579610696 28.017240037965426 20.066828486517604 82-83 20.816393529344953 26.022028357737543 33.01748670098971 20.1440914119278 84-85 18.653522866680202 29.53053078516624 31.11103850489275 20.70490784326081 86-87 19.156607948021396 30.866341178044763 28.711301549960638 21.2657493239732 88-89 18.05932163167555 30.747468742973055 25.6167957355526 25.5764138897988 90-91 20.321781773791102 24.40765871432006 28.857113736217993 26.413445775670848 92-93 23.51017512525894 30.112941128882326 28.716481906005036 17.660401839853694 94-95 21.29999144281858 27.933674064738966 29.121021883046666 21.645312609395788 96-97 14.095608541755805 28.50986036018515 34.76012291415458 22.63440818390447 98-99 19.509436310737012 32.036702814556655 25.397056306691766 23.056804568014563 100-101 17.13613343394817 27.116373509253712 28.65150183207176 27.095991224726358 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1539.0 1 1129.5 2 940.0 3 1149.0 4 982.5 5 909.5 6 1392.5 7 2381.5 8 3171.5 9 3319.5 10 2778.5 11 2078.5 12 1869.5 13 1800.0 14 1687.5 15 1536.0 16 1371.0 17 1286.5 18 1250.0 19 1299.0 20 1351.0 21 1406.0 22 1550.0 23 1731.0 24 1896.0 25 2227.0 26 3031.5 27 3826.0 28 4159.5 29 4925.0 30 6238.5 31 7340.5 32 9565.5 33 12596.5 34 15673.0 35 17600.5 36 19957.5 37 22239.0 38 20888.5 39 18670.0 40 16462.0 41 14115.5 42 11992.0 43 11154.5 44 10921.5 45 10589.0 46 13516.0 47 47620.0 48 121818.0 49 112722.0 50 38400.5 51 10314.5 52 3338.0 53 1634.0 54 1124.5 55 906.5 56 802.0 57 733.5 58 683.5 59 577.0 60 450.0 61 398.0 62 365.5 63 341.5 64 305.0 65 287.5 66 240.0 67 175.0 68 135.5 69 105.5 70 96.5 71 82.0 72 61.0 73 69.0 74 70.5 75 46.0 76 31.0 77 25.5 78 18.5 79 15.0 80 14.0 81 12.0 82 11.0 83 9.0 84 4.5 85 2.0 86 1.5 87 1.5 88 1.5 89 3.5 90 2.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9917841379878994 2 0.011356900717351633 3 0.0048227934553137074 4 0.012134770629499005 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0035782015958779117 10-11 3.8893495607368605E-4 12-13 7.77869912147372E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0015557398242947442 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.002878118674945277 34-35 4.6672194728842323E-4 36-37 0.0027225446925158024 38-39 0.0 40-41 0.002644757701301065 42-43 0.00917886496333899 44-45 0.001866887789153693 46-47 0.0016335268155094815 48-49 0.008789930007265306 50-51 2.3336097364421162E-4 52-53 0.0014001658418652699 54-55 0.00840099505119162 56-57 0.003033692657374751 58-59 9.334438945768465E-4 60-61 0.004278284516810547 62-63 0.002255822745227379 64-65 0.015168463286873756 66-67 0.0024113967276568537 68-69 7.77869912147372E-5 70-71 0.0010112308857915839 72-73 0.0 74-75 0.0 76-77 0.0012445918594357953 78-79 0.008867716998480043 80-81 0.014157232401082171 82-83 0.009956734875486362 84-85 0.006923042218111611 86-87 0.008556569033621094 88-89 0.025747494092078018 90-91 0.021858144531341158 92-93 0.0019446747803684302 94-95 0.0071564031917558234 96-97 0.008712143016050567 98-99 0.007934273103903195 100-101 0.010267882840345311 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 642781.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.498717292514868 #Duplication Level Percentage of deduplicated Percentage of total 1 72.85047481088041 10.562384389084308 2 9.694726111915877 2.8112218625006027 3 4.205161221095553 1.829083311423331 4 2.3810290251622943 1.3808746680440152 5 1.5730457642577391 1.1403572912080475 6 1.158860453887011 1.0081194061429943 7 0.9024089275175707 0.9158640345623159 8 0.6631257041686786 0.7691577691313216 9 0.5633349428617416 0.7350870669792666 >10 4.84789956542733 14.473669881343723 >50 0.6534685337196202 6.5045482053763255 >100 0.43135361339127637 11.503606982782626 >500 0.02253339771446966 2.287248689678133 >1k 0.03540962497988089 12.804827771822753 >5k 0.008584151510274156 8.721944176943625 >10k+ 0.008584151510274156 22.552004492976614 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 44848 6.977181963997069 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 19421 3.021402312762823 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 19103 2.97192978635025 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13610 2.1173619008651468 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 12474 1.9406298568252638 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 12415 1.9314509918619251 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 12406 1.9300508260200597 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 10683 1.6619968542940753 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9201 1.4314362123335942 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8704 1.3541159430661454 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 8680 1.350382167487838 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7091 1.1031751094074032 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6057 0.9423116115753265 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5951 0.9258207694378023 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5320 0.8276535865248039 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5059 0.7870487771107111 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4953 0.7705579349731868 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4928 0.76666858541245 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4875 0.7584231643436878 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4546 0.7072393241243907 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4302 0.669279272411599 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4237 0.6591669635536831 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4009 0.6236960955597629 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3975 0.6184065801571609 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3782 0.5883808015482722 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3661 0.5695563496743059 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3550 0.5522876376246342 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2788 0.4337402630133747 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2579 0.4012253006856145 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2201 0.3424183353272732 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2021 0.31441501848996783 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1898 0.2952794186511425 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1873 0.2913900690904056 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1791 0.27863300253118867 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1745 0.27147659933943286 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1669 0.2596529766747928 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1567 0.24378443046698642 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1553 0.2416063947129738 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1499 0.23320539966178214 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1464 0.22776031027675053 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1437 0.22355981275115475 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1378 0.21438094778781577 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1305 0.20302404707046412 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1280 0.19913469750972726 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1255 0.1952453479489904 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1071 0.16661973518196713 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1059 0.16475284739281343 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1047 0.16288595960365973 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1009 0.1569741482713397 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 989 0.15386266862275022 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 941 0.14639511746613543 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 933 0.14515052560669964 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 824 0.12819296152188694 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 799 0.12430361196115007 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 797 0.12399246399629112 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 706 0.10983523159520894 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 704 0.10952408363034999 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 698 0.10859063973577315 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 698 0.10859063973577315 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 674 0.10485686415746577 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 668 0.10392342026288891 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 666 0.10361227229802998 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 664 0.10330112433317101 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.555739824294744E-4 0.0 14-15 0.0 0.0 0.0 2.3336097364421162E-4 0.0 16-17 0.0 0.0 0.0 3.111479648589488E-4 0.0 18-19 0.0 0.0 0.0 3.8893495607368605E-4 0.0 20-21 0.0 0.0 0.0 7.000829209326349E-4 0.0 22-23 0.0 0.0 0.0 8.556569033621093E-4 0.0 24-25 0.0 0.0 0.0 0.0012445918594357953 0.0 26-27 0.0 0.0 0.0 0.0020224617715831677 0.0 28-29 0.0 0.0 0.0 0.003811562569522123 0.0 30-31 0.0 0.0 0.0 0.007311977174185299 0.0 32-33 0.0 0.0 0.0 0.012212557620713743 0.0 34-35 0.0 0.0 0.0 0.016335268155094815 0.0 36-37 0.0 0.0 0.0 0.019757895768543254 0.0 38-39 0.0 0.0 0.0 0.02512519816236012 0.0 40-41 0.0 0.0 0.0 0.033292832239907526 0.0 42-43 0.0 0.0 0.0 0.042860632159320206 0.0 44-45 0.0 0.0 0.0 0.0675191083743919 0.0 46-47 0.0 0.0 0.0 0.12383689001386164 0.0 48-49 0.0 0.0 0.0 0.15977447995507024 0.0 50-51 0.0 0.0 0.0 0.2059021657454094 0.0 52-53 0.0 0.0 0.0 0.25599698808770016 0.0 54-55 0.0 0.0 0.0 0.3121591957447404 0.0 56-57 0.0 0.0 0.0 0.38504560651294917 0.0 58-59 0.0 0.0 0.0 0.4840684463293097 0.0 60-61 0.0 0.0 0.0 0.6011378681074891 0.0 62-63 0.0 0.0 0.0 0.7404543693730836 0.0 64-65 0.0 0.0 0.0 0.8302205572348902 0.0 66-67 0.0 0.0 0.0 1.0039966956086133 0.0 68-69 0.0 0.0 0.0 1.2132437019762563 0.0 70-71 0.0 0.0 0.0 1.3475818358041076 0.0 72-73 0.0 0.0 0.0 1.5530172796022286 0.0 74-75 0.0 0.0 0.0 1.7408728633858188 0.0 76-77 0.0 0.0 0.0 1.85747556321671 0.0 78-79 0.0 0.0 0.0 2.076990452424698 0.0 80-81 0.0 0.0 0.0 2.29557189773811 0.0 82-83 0.0 0.0 0.0 2.5191317104892645 0.0 84-85 0.0 0.0 0.0 2.895309600003734 0.0 86-87 0.0 0.0 0.0 3.3731550870358644 0.0 88-89 0.0 0.0 0.0 3.9547684203484548 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 25 0.0050214105 56.99673 2 ATGTATT 60 2.1771484E-8 55.953342 1 GGTAATC 40 5.394123E-4 47.48619 8 ATGAGAG 40 5.394123E-4 47.48619 5 GGGATGC 40 5.394123E-4 47.48619 7 CTAGATA 75 1.362987E-7 44.327343 4 GATAGTC 45 9.6356816E-4 42.20995 7 GACATAT 175 0.0 41.10858 1 CATATAG 175 0.0 40.711952 3 GTATGGT 60 7.82909E-5 39.966675 1 CTTATGT 120 8.731149E-11 39.581066 2 TGTATGG 75 6.920245E-6 37.99782 2 AGATAGT 50 0.0016175946 37.988953 6 GTCAAGT 25 0.0016181967 37.988953 10-11 TAGTCAA 50 0.0016175946 37.988953 9 TAGGCAT 145 1.6370905E-11 36.02401 5 TACCTGG 80 1.0772923E-5 35.622955 2 TACTCCC 150 2.5465852E-11 34.823208 5 ACATATA 205 0.0 34.75411 2 GGAGACT 55 0.0025824625 34.53541 8 >>END_MODULE