##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936649_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 652601 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.631764278632733 31.0 31.0 34.0 30.0 34.0 2 31.556528414758787 31.0 31.0 34.0 30.0 34.0 3 31.64609462749827 33.0 31.0 34.0 30.0 34.0 4 35.18316858233438 37.0 35.0 37.0 33.0 37.0 5 35.22917372176874 37.0 35.0 37.0 33.0 37.0 6 35.46339493810154 37.0 35.0 37.0 33.0 37.0 7 35.37844410290514 37.0 35.0 37.0 33.0 37.0 8 35.34531972828727 37.0 35.0 37.0 33.0 37.0 9 36.76467244150714 39.0 37.0 39.0 32.0 39.0 10-11 36.67468560422065 39.0 37.0 39.0 32.0 39.0 12-13 36.66250664648078 39.0 37.0 39.0 32.0 39.0 14-15 37.46292527899896 39.0 37.0 41.0 32.0 41.0 16-17 37.50130477887714 39.0 37.0 41.0 32.0 41.0 18-19 37.683528679851854 40.0 37.0 41.0 32.0 41.0 20-21 37.569239090960636 39.5 37.0 41.0 32.0 41.0 22-23 37.42703581514586 39.0 36.5 41.0 32.0 41.0 24-25 37.496107115986646 39.5 37.0 41.0 32.0 41.0 26-27 37.389702896563136 39.0 36.5 41.0 31.5 41.0 28-29 37.38956805153532 39.0 36.5 41.0 32.0 41.0 30-31 37.312692594709475 39.0 36.0 41.0 32.0 41.0 32-33 37.022613357932336 39.0 35.0 41.0 31.0 41.0 34-35 36.623449856803774 38.5 35.0 40.0 30.5 41.0 36-37 36.29676249346844 38.5 35.0 40.0 30.0 41.0 38-39 36.0160810357324 38.0 35.0 40.0 30.0 41.0 40-41 36.093767861219945 38.0 35.0 40.0 30.0 41.0 42-43 35.789975038346554 38.0 35.0 40.0 30.0 41.0 44-45 35.37238297213764 38.0 35.0 40.0 28.0 41.0 46-47 34.81680077106839 38.0 34.0 40.0 25.0 41.0 48-49 34.45704572932006 38.0 33.5 40.0 23.5 41.0 50-51 33.36686198764636 37.0 32.0 39.0 22.5 40.5 52-53 33.62716115972853 37.5 33.0 39.5 23.0 40.0 54-55 34.167700478546614 38.0 34.0 40.0 23.0 41.0 56-57 33.82973286893523 38.0 33.5 40.0 21.0 41.0 58-59 33.57919770273107 38.0 33.0 40.0 19.5 41.0 60-61 32.879005701799414 37.0 32.0 40.0 14.0 41.0 62-63 32.69876157100587 36.5 31.5 40.0 13.0 41.0 64-65 32.35877818146157 36.0 31.5 39.0 10.0 40.5 66-67 31.575760686851538 36.0 30.5 39.0 7.0 40.0 68-69 31.018873706905136 35.0 30.0 38.0 2.0 40.0 70-71 30.219538431599094 35.0 29.0 37.0 2.0 39.0 72-73 29.72731577181157 34.5 29.0 37.0 2.0 39.0 74-75 29.358501595921553 34.0 29.0 36.0 2.0 38.5 76-77 28.936012969639947 34.0 29.0 36.0 2.0 37.0 78-79 28.33651955789219 34.0 27.0 35.0 2.0 37.0 80-81 27.5325551140743 33.5 26.0 35.0 2.0 36.5 82-83 26.971874085390617 33.0 25.5 35.0 2.0 36.0 84-85 26.542193468903662 32.5 25.0 35.0 2.0 35.5 86-87 26.568918067854632 33.0 25.0 35.0 2.0 35.0 88-89 25.880210879235552 32.5 22.0 35.0 2.0 35.0 90-91 25.200917559121116 32.0 19.5 35.0 2.0 35.0 92-93 24.77641468523646 32.0 17.5 35.0 2.0 35.0 94-95 24.254149166182707 31.5 7.5 35.0 2.0 35.0 96-97 23.557464668304217 31.0 2.0 34.0 2.0 35.0 98-99 22.997472421893317 31.0 2.0 34.0 2.0 35.0 100-101 20.703555464977835 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.373696935712843 1101 2 0.45487611505519965 1101 3 0.4262882708364657 1101 4 0.38676036883995835 1101 5 0.3153351828853346 1101 6 0.29307124211400293 1101 7 0.4571491801319212 1101 8 0.39811953521828514 1101 9 0.6344274684398243 1101 10-11 0.6059619548001862 1101 12-13 0.7549942823544384 1101 14-15 0.8954949675066359 1101 16-17 0.8488257327826858 1101 18-19 0.8264520485797817 1101 20-21 0.9222278579117855 1101 22-23 0.8929968340979286 1101 24-25 0.8491709380964423 1101 26-27 0.7125192072856805 1101 28-29 0.6557497120841447 1101 30-31 0.6191251933353143 1101 32-33 0.6305742123384661 1101 34-35 0.8179366917800976 1101 36-37 0.8819318635132518 1101 38-39 0.9802165471225237 1101 40-41 0.835401577499745 1101 42-43 0.9244723135415782 1101 44-45 0.9473999403097437 1101 46-47 0.862174816150663 1101 48-49 1.0455892338332262 1101 50-51 0.9102349711705173 1101 52-53 0.8788339830963352 1101 54-55 1.0917540666754775 1101 56-57 0.6227310762001323 1101 58-59 0.7358472978590243 1101 60-61 0.608711687490846 1101 62-63 0.5228452932680554 1101 64-65 0.7523002969291497 1101 66-67 0.7947221337179506 1101 68-69 0.8861846193148182 1101 70-71 0.9816834837482773 1101 72-73 0.6904815355092815 1101 74-75 0.7008921187837345 1101 76-77 0.7038802403985862 1101 78-79 0.45371096076643624 1101 80-81 0.9390801329156631 1101 82-83 1.208508748222254 1101 84-85 1.30609574547222 1101 86-87 1.3208772276984426 1101 88-89 1.305939552982231 1101 90-91 1.2409104472846053 1101 92-93 1.1874886761812569 1101 94-95 0.932526542069759 1101 96-97 1.0961550389605748 1101 98-99 1.1549135029984718 1101 100-101 1.6272500521283781 1102 1 0.30183149228776784 1102 2 0.4487599071041295 1102 3 0.2160637105275356 1102 4 0.2707627510415094 1102 5 0.4518462029308026 1102 6 0.41353785111626706 1102 7 0.48019367152716796 1102 8 0.381037637586779 1102 9 0.5658895446419123 1102 10-11 0.39661181371146625 1102 12-13 0.31534693326265284 1102 14-15 0.560542776968326 1102 16-17 0.41157594666089636 1102 18-19 0.5276133718360327 1102 20-21 0.5809065868370951 1102 22-23 0.7369815388514667 1102 24-25 0.43596886217794406 1102 26-27 0.5297183243585977 1102 28-29 0.5292637902335855 1102 30-31 0.3639353970986221 1102 32-33 0.20574472408640787 1102 34-35 -0.2895920577058817 1102 36-37 0.06223474342477431 1102 38-39 0.19492900499678711 1102 40-41 0.27998466859531845 1102 42-43 0.3250000373574409 1102 44-45 -0.03154664449237288 1102 46-47 -0.06022874958911473 1102 48-49 0.09198190005560036 1102 50-51 0.03780564551316701 1102 52-53 -0.17184333730504164 1102 54-55 -0.08458438555524594 1102 56-57 -0.09116131824328733 1102 58-59 -0.11057987247311019 1102 60-61 -0.1044374506154 1102 62-63 0.305991689951874 1102 64-65 0.21253667371402685 1102 66-67 0.27276357646240257 1102 68-69 0.03739297214533721 1102 70-71 0.09507202365724154 1102 72-73 -0.11124863289913733 1102 74-75 -0.18959041882810723 1102 76-77 0.11404193705546462 1102 78-79 0.3387000187097051 1102 80-81 0.4091655424038194 1102 82-83 0.3743317185061805 1102 84-85 0.2773557126696815 1102 86-87 0.3869998638124308 1102 88-89 0.3526985299590102 1102 90-91 0.6271745029827471 1102 92-93 0.7635702382248439 1102 94-95 0.9512544748009226 1102 96-97 0.9171106137375986 1102 98-99 1.27243899113709 1102 100-101 1.7526547664296253 1103 1 0.3522902078840957 1103 2 0.39218498355672793 1103 3 0.3506202854510825 1103 4 0.48177192535344204 1103 5 0.36010308366475385 1103 6 0.10619840157498572 1103 7 0.16979611801034622 1103 8 0.20060950303020775 1103 9 0.48790789326576345 1103 10-11 0.3866729757909724 1103 12-13 0.5248270555868118 1103 14-15 0.4366895659591492 1103 16-17 0.4360407784651841 1103 18-19 0.43128309660667696 1103 20-21 0.499102305491526 1103 22-23 0.3279601321236356 1103 24-25 0.627100360648889 1103 26-27 0.4456204650313822 1103 28-29 0.32437082387272653 1103 30-31 0.37846139098241594 1103 32-33 0.7195061919763148 1103 34-35 0.5995516731809616 1103 36-37 0.8657515629966355 1103 38-39 0.9464580569845609 1103 40-41 0.6760091334271223 1103 42-43 0.7684251138100464 1103 44-45 0.9195236918990659 1103 46-47 1.0383951036218946 1103 48-49 1.1317372517375475 1103 50-51 1.0309249115682135 1103 52-53 1.129379904285166 1103 54-55 1.1677091924264076 1103 56-57 0.9677071832857607 1103 58-59 0.9582274669764246 1103 60-61 0.6524432833295535 1103 62-63 0.8258693657928546 1103 64-65 0.9373073159158594 1103 66-67 0.6580846773798292 1103 68-69 0.8898394553257631 1103 70-71 1.0782524517918013 1103 72-73 1.1540418869785398 1103 74-75 1.232427929795744 1103 76-77 1.1553263407251926 1103 78-79 1.257660263358023 1103 80-81 1.3548841968380714 1103 82-83 1.4507843178945627 1103 84-85 1.5877532661864997 1103 86-87 1.7677338087665575 1103 88-89 1.9345028113045757 1103 90-91 1.87258734701944 1103 92-93 1.7964448559618482 1103 94-95 1.5774012637917494 1103 96-97 1.5470800326978456 1103 98-99 1.5637233948068179 1103 100-101 2.0561715860014864 1104 1 0.3692259968156755 1104 2 0.3296898259344836 1104 3 0.21664707462420907 1104 4 0.3700052892222345 1104 5 0.3931884872569995 1104 6 0.3111346644898916 1104 7 0.36873239004031433 1104 8 0.3865978266332064 1104 9 0.5559153858483796 1104 10-11 0.5052679342490691 1104 12-13 0.2596345226472536 1104 14-15 0.3906174749178888 1104 16-17 0.5329703691532259 1104 18-19 0.6084252505874446 1104 20-21 0.7298968656213347 1104 22-23 0.3021269013347734 1104 24-25 0.375758167473343 1104 26-27 0.10910369559236699 1104 28-29 0.4400113622084092 1104 30-31 0.4351867130859901 1104 32-33 0.24998582664454005 1104 34-35 0.3094693185776336 1104 36-37 0.29518159804405997 1104 38-39 0.37357970207131075 1104 40-41 0.2774233356083542 1104 42-43 0.3624504172277412 1104 44-45 0.37000040974681525 1104 46-47 0.4194355426033738 1104 48-49 0.3401771173815078 1104 50-51 0.3299388895188784 1104 52-53 0.2822976362749401 1104 54-55 0.2936258260509703 1104 56-57 0.2446695426289338 1104 58-59 0.40893206142444427 1104 60-61 0.3770555512942053 1104 62-63 0.4590313359227949 1104 64-65 -0.07261557064261126 1104 66-67 0.20399155788590306 1104 68-69 -0.314610291047579 1104 70-71 -0.022628519144820558 1104 72-73 -0.15973279775418447 1104 74-75 -0.19042444718506957 1104 76-77 0.13104608896712122 1104 78-79 -0.15981080770757217 1104 80-81 0.09065786068362058 1104 82-83 0.005987424308376177 1104 84-85 0.12404261477844969 1104 86-87 0.1759679018404796 1104 88-89 0.3146533921620289 1104 90-91 0.31187076626225263 1104 92-93 0.5117757099977709 1104 94-95 0.41452646375346447 1104 96-97 0.04467935271406631 1104 98-99 0.2522479849707544 1104 100-101 0.5105484118627466 1105 1 0.2349337381261165 1105 2 0.27237234431613544 1105 3 0.2345645477007956 1105 4 0.21356513663936738 1105 5 0.37490236964590196 1105 6 0.2343689358437402 1105 7 0.3145371478026462 1105 8 0.3154336966455489 1105 9 0.3305746706330268 1105 10-11 0.32560429990848405 1105 12-13 0.2649124334217774 1105 14-15 0.22151528170769552 1105 16-17 0.49192382328508444 1105 18-19 0.36964362077399926 1105 20-21 0.4065877414443193 1105 22-23 0.5013691569897532 1105 24-25 0.4877732818992584 1105 26-27 0.3772836031165312 1105 28-29 0.36463816581303377 1105 30-31 0.42513552993933956 1105 32-33 0.206869027018584 1105 34-35 0.06310350181644964 1105 36-37 0.11802092522282948 1105 38-39 0.1732247693152189 1105 40-41 -0.18008701004085026 1105 42-43 2.1139658635149772E-5 1105 44-45 -0.036697681618584 1105 46-47 0.05189019820171126 1105 48-49 0.25490896220014747 1105 50-51 0.37993967626025693 1105 52-53 0.11988386875027857 1105 54-55 0.1007595473601981 1105 56-57 0.3226513486724585 1105 58-59 0.17238814889210374 1105 60-61 0.05346657196751892 1105 62-63 0.1587737343151403 1105 64-65 0.06600713741114816 1105 66-67 0.24154946767139407 1105 68-69 0.3019881539018776 1105 70-71 0.373947582349313 1105 72-73 0.41547518295041996 1105 74-75 0.5814115599450531 1105 76-77 0.4126567958430165 1105 78-79 0.8265886815848766 1105 80-81 0.514704723496159 1105 82-83 0.45321281222807386 1105 84-85 0.7063897115903508 1105 86-87 0.8311998444398228 1105 88-89 0.7793905193871034 1105 90-91 0.6751632115219124 1105 92-93 0.8733575547040147 1105 94-95 1.0490895862625855 1105 96-97 1.1220070394920931 1105 98-99 0.8917776934493524 1105 100-101 1.2350360746339213 1106 1 -0.07158042560649847 1106 2 0.08606052285130161 1106 3 0.14534219055357767 1106 4 0.1768120199850216 1106 5 -0.012451464749112517 1106 6 -0.3154737898522342 1106 7 0.0299531954349348 1106 8 0.08661145618206945 1106 9 0.3119866795606896 1106 10-11 0.0010572973916467276 1106 12-13 0.23976889659291345 1106 14-15 0.45949696959678477 1106 16-17 0.19510390745114847 1106 18-19 0.19102478539529955 1106 20-21 0.08982643883770436 1106 22-23 -0.004771437871184503 1106 24-25 -0.15590148705111062 1106 26-27 -0.2028878496186337 1106 28-29 0.010121984989417854 1106 30-31 -0.11666031422003442 1106 32-33 -0.23733534138544599 1106 34-35 -0.1596765108921332 1106 36-37 -0.39556617263095717 1106 38-39 -0.31032983347387955 1106 40-41 -0.4049824808574769 1106 42-43 -0.49758752207384305 1106 44-45 -0.3756074122736237 1106 46-47 -0.01061135645082345 1106 48-49 0.013315957797367162 1106 50-51 -0.1534565215684225 1106 52-53 -0.05516887637037371 1106 54-55 0.14529723326837285 1106 56-57 -0.12062135886517922 1106 58-59 -0.29858564023143686 1106 60-61 -0.10385861159453924 1106 62-63 0.49533509164552214 1106 64-65 0.25227712458950435 1106 66-67 0.1690596944584506 1106 68-69 0.21522836637482712 1106 70-71 0.3461345657806767 1106 72-73 0.6265212436304886 1106 74-75 0.4907804049343234 1106 76-77 0.7350250234939004 1106 78-79 0.6374027315734594 1106 80-81 0.993313835229884 1106 82-83 0.877809337494849 1106 84-85 0.9003515541984584 1106 86-87 1.0023687848564933 1106 88-89 0.9279372867274134 1106 90-91 0.9227125137938543 1106 92-93 1.2971795803427781 1106 94-95 1.4173115838610748 1106 96-97 1.6573242225295353 1106 98-99 2.080964774117163 1106 100-101 2.4485808859386786 1107 1 0.0774006394165454 1107 2 0.007582667761752759 1107 3 -0.09767946101380076 1107 4 0.07429917384398976 1107 5 0.13122883185472034 1107 6 0.28741066535605597 1107 7 0.2326702129945346 1107 8 0.42143336146874333 1107 9 0.26521519350884404 1107 10-11 0.06814661598021132 1107 12-13 0.18191427689421147 1107 14-15 -0.18228897503774277 1107 16-17 -0.12886551809248914 1107 18-19 0.1568901433146337 1107 20-21 0.23585705600775242 1107 22-23 0.4008580123609704 1107 24-25 0.1952477246678157 1107 26-27 -0.16830044246750475 1107 28-29 0.0442929337030904 1107 30-31 0.09029390098006473 1107 32-33 -0.09675926190382 1107 34-35 0.07336829149300428 1107 36-37 -0.0553911746598601 1107 38-39 -0.08842262468511564 1107 40-41 0.007695795389018656 1107 42-43 -0.053146281155846964 1107 44-45 -0.31200353045385043 1107 46-47 -0.010913555778508055 1107 48-49 -0.01897238521061695 1107 50-51 -0.18282218061924738 1107 52-53 0.028129218172097126 1107 54-55 0.09472636485642738 1107 56-57 0.10671369155833332 1107 58-59 -0.03022623019286641 1107 60-61 0.11685688535783356 1107 62-63 0.29498120875791045 1107 64-65 0.2159149637895652 1107 66-67 0.07802508355877436 1107 68-69 -0.01848276951022143 1107 70-71 0.22793618633791723 1107 72-73 0.10669355063937758 1107 74-75 0.3376123308517087 1107 76-77 0.29790063696921365 1107 78-79 0.4301164174655554 1107 80-81 0.6717769540978011 1107 82-83 0.706606477386444 1107 84-85 0.949099616064963 1107 86-87 0.9174240777228846 1107 88-89 0.7863873352074471 1107 90-91 0.5369865733452741 1107 92-93 0.6681874524750882 1107 94-95 0.7795236402260564 1107 96-97 0.8551644050023981 1107 98-99 1.1783957594791836 1107 100-101 1.4091604252288583 1108 1 -0.224530538437822 1108 2 -0.21720660281776816 1108 3 -0.24592229537185162 1108 4 -0.07934498275437818 1108 5 -0.0420511405389874 1108 6 0.031107719983687332 1108 7 -0.2037216953323835 1108 8 -0.21216677656271088 1108 9 -0.16631405190518933 1108 10-11 -0.3670175278249985 1108 12-13 -0.08028341107895187 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-2.7702025453578436 2316 74-75 -2.472344774049219 2316 76-77 -2.4961651599055728 2316 78-79 -3.605401227602652 2316 80-81 -3.1294730859162883 2316 82-83 -2.8807499863745143 2316 84-85 -2.5596223111962892 2316 86-87 -3.4243327921238738 2316 88-89 -3.2178918853639082 2316 90-91 -3.296993844904808 2316 92-93 -3.7942073104814753 2316 94-95 -4.328182843884925 2316 96-97 -5.275369542947278 2316 98-99 -6.247673073893022 2316 100-101 -6.242241439656151 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1015.0 3 353.0 4 260.0 5 279.0 6 324.0 7 405.0 8 507.0 9 746.0 10 1134.0 11 1605.0 12 2569.0 13 3773.0 14 4368.0 15 5770.0 16 7823.0 17 10288.0 18 8444.0 19 5694.0 20 5958.0 21 6359.0 22 7052.0 23 8231.0 24 9282.0 25 9923.0 26 11144.0 27 12864.0 28 14880.0 29 17628.0 30 20918.0 31 25212.0 32 31448.0 33 39635.0 34 51030.0 35 68942.0 36 84334.0 37 95372.0 38 67465.0 39 9567.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.12615192186245 19.450388419161605 14.447997204117796 15.975462454858153 2 13.315452288262883 26.98724826042976 33.80789626950311 25.889403181804248 3 28.985522794778028 26.85578275915975 30.520603119817803 13.638091326244417 4 10.077417178948659 16.346675660345543 45.24627860986341 28.32962855084239 5 8.859566013264283 43.638755254661355 34.97787753074609 12.523801201328268 6 23.63978636383147 40.16945566926864 16.175244203800567 20.015513763099317 7 22.130016587815774 37.765795985196675 22.30846591832417 17.79572150866338 8 22.926139063731156 24.16691449472203 21.07923241774919 31.827714023797625 9 20.975517781071844 24.415690326220087 27.76342797052507 26.845363922182997 10-11 24.623061651553364 24.206072405889973 26.188381023923224 24.98248491863344 12-13 32.562925536358804 24.970854855263028 24.070632041493795 18.39558756688437 14-15 25.002931647769994 26.346584381253702 33.32628204980812 15.324201921168182 16-17 17.149138036097398 30.39006252313862 31.68038312572769 20.78041631503629 18-19 15.841084781100648 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24.61828902768669 44-45 19.711348389331064 26.507617386827174 28.59194037260989 25.18909385123187 46-47 23.975577670531468 30.40279681489183 28.848014747176485 16.77361076740022 48-49 21.429014712766616 28.816811000365462 29.62910466114973 20.12506962571819 50-51 14.31700679438184 30.527215835782517 36.23133996733058 18.924437402505063 52-53 18.898125871554008 34.52212023231224 26.044179168518323 20.535574727615426 54-55 15.874043573071553 28.132322134856775 30.46513801817154 25.528496273900135 56-57 21.865502946337575 26.845005019118627 27.72499827587528 23.56449375866852 58-59 26.71051603204109 26.74315785924783 26.45658407109482 20.089742037616258 60-61 20.890175837580877 28.606785240309318 33.909574060508724 16.59346486160108 62-63 18.685873000670433 29.775998467579733 31.80645532037161 19.73167321137822 64-65 17.36949529651349 35.381879586454815 28.72528543474497 18.52333968228673 66-67 20.235443575297428 28.453288647479024 25.773133472938316 25.538134304285233 68-69 17.25697182305328 29.693188444777523 29.226982619963326 23.822857112205874 70-71 24.013080769460633 28.799299988813303 26.203680575455778 20.98393866627028 72-73 21.099392801731568 24.248472427069167 34.33677476200509 20.31536000919417 74-75 16.91177146639942 35.41262500421406 28.394996460193266 19.280607069193263 76-77 18.91413317150839 27.598432014368097 28.501111780273604 24.986323033849917 78-79 19.477340223057897 28.188642987835717 31.006365639644113 21.327651149462277 80-81 23.50867352763813 27.700195316241437 28.077571734084867 20.713559422035566 82-83 20.083072884541565 25.787227065118202 33.90219589171562 20.227504158624615 84-85 18.896284421666955 29.130523405507468 31.04800642456046 20.925185748265125 86-87 18.948359642092587 30.75818569933387 29.846867237434697 20.446587421138847 88-89 18.141160317088158 29.800264495535295 26.028138279369195 26.03043690800735 90-91 19.7814364237893 24.73983992742641 29.381040456404513 26.097683192379773 92-93 23.381320598713103 29.365386486886198 29.7513637941485 17.5019291202522 94-95 20.866546876975477 27.36078279625919 30.10217881867953 21.670491508085803 96-97 14.164648910411623 28.283170380359824 35.54027339320195 22.011907316026605 98-99 19.181175857523833 31.30667014069828 26.572433559145388 22.9397204426325 100-101 17.17902378532658 26.395473782797367 30.34457070068381 26.08093173119224 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 889.0 1 685.0 2 734.5 3 977.0 4 820.0 5 807.5 6 1293.5 7 2391.0 8 3540.0 9 3855.0 10 3018.5 11 2111.0 12 1946.0 13 1888.5 14 1785.5 15 1623.5 16 1471.0 17 1377.0 18 1286.0 19 1263.5 20 1317.5 21 1422.5 22 1576.0 23 1753.5 24 1937.5 25 2276.0 26 3132.0 27 3986.5 28 4290.5 29 4939.5 30 6290.5 31 7590.5 32 9702.0 33 12942.0 34 16240.0 35 18121.0 36 20449.5 37 22713.5 38 20976.5 39 18437.0 40 16345.5 41 14367.5 42 12490.5 43 11415.0 44 11410.0 45 11621.5 46 15839.5 47 47760.0 48 117945.5 49 111730.5 50 40713.0 51 11780.5 52 4212.0 53 2185.5 54 1444.5 55 1136.0 56 963.0 57 830.0 58 706.5 59 614.0 60 538.0 61 450.0 62 391.5 63 380.0 64 317.5 65 304.0 66 297.0 67 203.5 68 129.0 69 94.5 70 76.5 71 70.5 72 69.0 73 83.0 74 79.5 75 56.0 76 34.5 77 23.0 78 20.5 79 14.0 80 11.5 81 12.5 82 9.5 83 4.5 84 2.5 85 1.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03263862605175291 2 0.021605851048343474 3 0.019460589242124973 4 0.04459079897211313 5 0.04949425452918398 6 0.04244553716589462 7 0.048268390639916274 8 0.04091320730430998 9 0.038308246539616085 10-11 0.046889293764490095 12-13 0.040223658866596894 14-15 0.03639283421263529 16-17 0.04328831858976618 18-19 0.046506211299093936 20-21 0.012028789413439451 22-23 0.019077506776728814 24-25 0.006129319446338574 26-27 0.011032775003409434 28-29 0.007585032814843986 30-31 0.008044731773319378 32-33 0.0036009751747239126 34-35 0.00942382864874556 36-37 0.009730294621062487 38-39 0.011032775003409434 40-41 0.002528344271614662 42-43 0.0019920288200600367 44-45 0.002298494792376965 46-47 2.2984947923769655E-4 48-49 6.895484377130896E-4 50-51 4.596989584753931E-4 52-53 0.004750222570912395 54-55 0.006435785418655503 56-57 0.01471036667121258 58-59 0.00980691111414172 60-61 0.010266610072617113 62-63 0.005899469967100878 64-65 0.0 66-67 0.006972100870210129 68-69 0.015246682122767205 70-71 0.006129319446338574 72-73 0.002068645313139269 74-75 0.0035243586816446804 76-77 0.006742251390972433 78-79 0.005363154515546253 80-81 0.010649692538013273 82-83 0.006359168925576272 84-85 0.016089463546638757 86-87 0.00459698958475393 88-89 0.005822853474021645 90-91 0.0033711256954862164 92-93 0.01555314809508413 94-95 0.010496459551854808 96-97 0.009347212155666326 98-99 0.021605851048343474 100-101 0.03432418889949602 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 652601.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.407011441153223 #Duplication Level Percentage of deduplicated Percentage of total 1 74.3572030919589 11.456222787699645 2 9.092386773416749 2.801730140908442 3 3.8878860433706643 1.7970211425633529 4 2.260915336946106 1.3933579385522978 5 1.5889402382365674 1.224041021490976 6 1.0769594907955564 0.9955636317807409 7 0.8599676527591962 0.9274672025557833 8 0.684974235045633 0.8442724700994596 9 0.5499793128103472 0.7626183808379945 >10 4.5718280330343735 14.625633965598475 >50 0.5999774321567419 6.38041972202519 >100 0.3979850299973055 11.178136691252657 >500 0.025999022060125485 2.802738097819149 >1k 0.025999022060125485 9.608067212642556 >5k 0.01199954864313484 12.598609715763024 >10k+ 0.006999736708495323 20.604099878410263 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 38534 5.904679888630265 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 25855 3.9618388571270957 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 20081 3.077071595048123 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 15431 2.3645382094112635 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11861 1.8174964488255458 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 11562 1.7716797859641649 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 10413 1.5956150848680895 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9920 1.5200712226919666 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9805 1.5024494292837431 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8239 1.262486572959588 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8005 1.2266300541985071 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7000 1.0726309031092505 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6150 0.9423828648745558 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6002 0.9197043829231031 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5700 0.8734280211032468 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5495 0.8420152589407617 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5231 0.8015617505949271 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5214 0.7989567898302331 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5014 0.7683101925985403 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4788 0.7336795377267274 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4515 0.6918469325054666 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4166 0.6383686203361626 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4131 0.6330054658206163 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3693 0.5658894178832089 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3579 0.5484208574611439 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3450 0.5286538022467021 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3382 0.5182339591879265 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3234 0.49555547723647375 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2814 0.4311976230499187 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2112 0.3236280667666767 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2048 0.31382115565253504 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1955 0.2995704879397978 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1930 0.29573966328583623 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1876 0.28746508203327914 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1607 0.24624540875665224 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1593 0.24410014695043372 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1558 0.23873699243488747 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1545 0.23674496361482744 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1366 0.2093162590924623 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1334 0.20441280353539149 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1316 0.20165460978453909 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1171 0.17943582679156175 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1112 0.17039508060821237 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1063 0.1628866642864476 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1026 0.15721704379858442 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 994 0.15231358824151356 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 994 0.15231358824151356 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 933 0.14296637608584725 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 927 0.14204697816889647 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 879 0.1346917948332902 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 839 0.1285624753869516 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 838 0.12840924240079313 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 815 0.12488488371914847 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 739 0.11323917677110516 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 712 0.10910188614482663 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 695 0.10649692538013272 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 682 0.1045048965600727 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 661 0.10128700385074493 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.5323298615846435E-4 0.0 8 0.0 0.0 0.0 1.5323298615846435E-4 0.0 9 0.0 0.0 0.0 1.5323298615846435E-4 0.0 10-11 0.0 0.0 0.0 1.5323298615846435E-4 0.0 12-13 0.0 0.0 0.0 1.5323298615846435E-4 0.0 14-15 0.0 0.0 0.0 1.5323298615846435E-4 0.0 16-17 0.0 0.0 0.0 2.2984947923769652E-4 0.0 18-19 0.0 0.0 0.0 3.064659723169287E-4 0.0 20-21 0.0 0.0 0.0 3.064659723169287E-4 0.0 22-23 0.0 0.0 0.0 0.0011492473961884826 0.0 24-25 0.0 0.0 0.0 0.0019920288200600367 0.0 26-27 0.0 0.0 0.0 0.0026815772577731263 0.0 28-29 0.0 0.0 0.0 0.004826839063991627 0.0 30-31 0.0 0.0 0.0 0.008504430731794773 0.0 32-33 0.0 0.0 0.0 0.012488488371914845 0.0 34-35 0.0 0.0 0.0 0.017162094449748005 0.0 36-37 0.0 0.0 0.0 0.02145261806218501 0.0 38-39 0.0 0.0 0.0 0.026509306605414333 0.0 40-41 0.0 0.0 0.0 0.03654606719879375 0.0 42-43 0.0 0.0 0.0 0.04964748751534245 0.0 44-45 0.0 0.0 0.0 0.07516077971072677 0.0 46-47 0.0 0.0 0.0 0.1359176587225579 0.0 48-49 0.0 0.0 0.0 0.1783631958884525 0.0 50-51 0.0 0.0 0.0 0.2297728627446173 0.0 52-53 0.0 0.0 0.0 0.2837108738723968 0.0 54-55 0.0 0.0 0.0 0.3447742188565448 0.0 56-57 0.0 0.0 0.0 0.4247618376312632 0.0 58-59 0.0 0.0 0.0 0.5195364395702734 0.0 60-61 0.0 0.0 0.0 0.6432720758932333 0.0 62-63 0.0 0.0 0.0 0.7819479283666436 0.0 64-65 0.0 0.0 0.0 0.8675285511361459 0.0 66-67 0.0 0.0 0.0 1.0416778399052407 0.0 68-69 0.0 0.0 0.0 1.2680795769543718 0.0 70-71 0.0 0.0 0.0 1.4059126480039104 0.0 72-73 0.0 0.0 0.0 1.6051921465029935 0.0 74-75 0.0 0.0 0.0 1.8049313439605519 0.0 76-77 0.0 0.0 0.0 1.9170978898285476 0.0 78-79 0.0 0.0 0.0 2.133462866284299 0.0 80-81 0.0 0.0 0.0 2.357719341527212 0.0 82-83 0.0 0.0 0.0 2.5833549136455507 0.0 84-85 0.0 0.0 0.0 2.9614573069915613 0.0 86-87 0.0 0.0 0.0 3.4591580460342537 0.0 88-89 0.0 0.0 0.0 4.053931881808333 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATGCC 40 7.5260086E-6 59.396034 8 GGGATGC 50 4.2287502E-7 57.02019 7 GACATAT 135 0.0 52.79647 1 AAGTTCG 25 2.8149096E-5 47.535046 14-15 AGTTCGA 25 2.8149096E-5 47.535046 14-15 GTTCGAC 25 2.8161858E-5 47.531403 16-17 ATGGGCG 40 5.375025E-4 47.520473 5 CGACCGT 25 2.8200173E-5 47.52047 18-19 AGATAGT 50 2.8197313E-5 47.516827 6 CTTATGT 190 0.0 47.516827 2 TACCTGG 100 1.2732926E-11 47.516827 2 TAGTCAA 50 2.8197313E-5 47.516827 9 GACCGTC 25 2.825773E-5 47.504078 20-21 GCTTATG 210 0.0 45.254124 1 TATGTAC 200 0.0 45.144447 4 GGCATTC 125 3.6379788E-12 41.81481 7 CCTTTAG 140 0.0 40.72871 1 TAGGCAT 130 5.456968E-12 40.20963 5 TCGACCG 30 8.2703446E-5 39.600395 18-19 GTATGGT 60 8.269528E-5 39.59736 1 >>END_MODULE