##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936649_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 652601 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.73089835902795 31.0 31.0 34.0 30.0 34.0 2 31.771475986092575 31.0 31.0 34.0 30.0 34.0 3 31.895485909460756 31.0 31.0 34.0 30.0 34.0 4 35.55094154008345 37.0 35.0 37.0 33.0 37.0 5 35.37405244552184 37.0 35.0 37.0 33.0 37.0 6 35.61693745489204 37.0 35.0 37.0 33.0 37.0 7 35.57387438879193 37.0 35.0 37.0 33.0 37.0 8 35.6174216711283 37.0 35.0 37.0 33.0 37.0 9 37.1295339725192 39.0 37.0 39.0 33.0 39.0 10-11 36.97249774364428 39.0 37.0 39.0 32.5 39.0 12-13 36.947350678285815 39.0 37.0 39.0 33.0 39.0 14-15 37.64470403814889 39.0 36.5 41.0 32.0 41.0 16-17 37.773633506537685 39.0 37.0 41.0 32.5 41.0 18-19 38.17518590992046 40.0 37.0 41.0 33.0 41.0 20-21 38.26480652037003 40.0 37.5 41.0 33.5 41.0 22-23 38.1480261292888 40.0 37.0 41.0 33.5 41.0 24-25 37.9004882002939 39.5 37.0 41.0 32.5 41.0 26-27 37.55279719154583 39.0 36.5 41.0 32.5 41.0 28-29 37.54017002732144 39.0 36.5 41.0 32.5 41.0 30-31 37.60624255862311 39.0 36.0 41.0 33.0 41.0 32-33 37.366626008847675 39.0 35.5 41.0 33.0 41.0 34-35 37.002663955464364 39.0 35.0 41.0 31.0 41.0 36-37 36.63715118426113 38.5 35.0 40.5 30.5 41.0 38-39 36.41422094051342 38.0 35.0 40.0 30.0 41.0 40-41 36.45022609527108 39.0 35.0 40.0 30.5 41.0 42-43 36.11938688417578 38.0 35.0 40.0 30.0 41.0 44-45 35.75561100887066 38.0 35.0 40.0 30.0 41.0 46-47 35.28575346957789 38.0 35.0 40.0 27.0 41.0 48-49 34.76819450169399 38.0 34.0 40.0 25.0 41.0 50-51 34.44436493355052 38.0 34.0 40.0 24.0 41.0 52-53 34.352629707891964 38.0 34.0 40.0 23.0 41.0 54-55 34.23858069478901 38.0 33.5 40.0 23.0 41.0 56-57 33.75848412736113 38.0 33.0 40.0 22.0 41.0 58-59 33.46874276931847 38.0 33.0 40.0 21.5 41.0 60-61 32.78074581559023 36.5 31.0 39.0 19.0 40.5 62-63 32.56420998435492 36.0 31.5 39.0 18.5 40.0 64-65 32.17517748210622 36.0 31.0 39.0 15.5 40.0 66-67 31.49074932462561 35.5 30.5 38.0 10.5 40.0 68-69 31.064344829382733 35.0 30.0 37.0 7.5 39.0 70-71 30.36055108711142 34.5 29.5 37.0 4.5 39.0 72-73 29.78660544498093 34.0 29.0 36.0 2.0 38.5 74-75 29.30899048576389 34.0 29.0 35.5 2.0 37.0 76-77 27.83607594839726 31.5 27.0 34.0 2.0 36.0 78-79 28.632501329296154 33.5 29.0 35.0 2.0 36.0 80-81 28.203052094618307 33.0 27.5 35.0 2.0 36.0 82-83 27.797486519328043 33.0 26.5 35.0 2.0 36.0 84-85 27.328908475469696 33.0 26.0 35.0 2.0 35.0 86-87 27.250143655924525 33.0 26.0 35.0 2.0 35.0 88-89 26.760230983403336 33.0 25.0 34.5 2.0 35.0 90-91 26.061804226472226 32.0 24.5 34.0 2.0 35.0 92-93 25.61327212186313 32.0 23.5 34.0 2.0 35.0 94-95 24.940704197511188 31.0 19.5 34.0 2.0 35.0 96-97 24.065051233448923 31.0 11.0 34.0 2.0 35.0 98-99 23.3062836250634 31.0 2.0 34.0 2.0 35.0 100-101 20.27534205433335 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18151408617593034 1101 2 0.3118873044083692 1101 3 0.2753608513827501 1101 4 0.13726511298545319 1101 5 0.04908504115668677 1101 6 -5.322324629801756E-4 1101 7 0.2698401017606571 1101 8 0.17196573925900793 1101 9 0.2913656155348363 1101 10-11 0.2698881166646956 1101 12-13 0.33380792579982455 1101 14-15 0.477684258484139 1101 16-17 0.36812650538242053 1101 18-19 0.4134368392501173 1101 20-21 0.47199263097284216 1101 22-23 0.4276534227555615 1101 24-25 0.4133957385507614 1101 26-27 0.34515359942114543 1101 28-29 0.17159614605380824 1101 30-31 0.3289914135398462 1101 32-33 0.26418104101011153 1101 34-35 0.2646771486864239 1101 36-37 0.02183290687236905 1101 38-39 0.2410378754046647 1101 40-41 0.2585624604364867 1101 42-43 0.3127957999551896 1101 44-45 0.2877590938422898 1101 46-47 0.3003728886664021 1101 48-49 0.2667508255104494 1101 50-51 0.23274749371426395 1101 52-53 0.4219032765033077 1101 54-55 0.6254386112049986 1101 56-57 0.4131020213867984 1101 58-59 0.32788468103863266 1101 60-61 0.7053344316166203 1101 62-63 0.8787781811976174 1101 64-65 0.796982153679437 1101 66-67 0.7802410931209813 1101 68-69 0.9326668815774504 1101 70-71 0.9803644678591574 1101 72-73 0.7808014312866653 1101 74-75 0.71717959476765 1101 76-77 0.6279985875500422 1101 78-79 0.7927083542180746 1101 80-81 0.7605261223345181 1101 82-83 1.3631641716829215 1101 84-85 1.3173530441615107 1101 86-87 0.8950409896465175 1101 88-89 0.9700008396132382 1101 90-91 1.187864834186275 1101 92-93 1.3005478985579657 1101 94-95 1.5557714036199322 1101 96-97 1.3589258016240073 1101 98-99 1.7201123678337886 1101 100-101 2.485786604824611 1102 1 0.24817319029799023 1102 2 0.12602831750575305 1102 3 0.1191068633221164 1102 4 0.1712705674707493 1102 5 0.18367942880326638 1102 6 0.15958337993770044 1102 7 0.2347640745544055 1102 8 0.2031021533839521 1102 9 0.0880891430837707 1102 10-11 0.2458179551571007 1102 12-13 0.2565636225228829 1102 14-15 0.21023132350784834 1102 16-17 0.27707228666417194 1102 18-19 0.4376487984690911 1102 20-21 0.19885242612240717 1102 22-23 0.2562956151372475 1102 24-25 0.24957802423534048 1102 26-27 -0.08413306355878802 1102 28-29 0.04651402154595985 1102 30-31 0.45691185557058134 1102 32-33 0.31955659224871624 1102 34-35 0.23763987006809373 1102 36-37 0.3321382918767952 1102 38-39 0.4162289478207626 1102 40-41 0.43536884511476615 1102 42-43 0.8366249053534318 1102 44-45 0.9129676546427916 1102 46-47 0.6108980601234677 1102 48-49 0.4372318506133652 1102 50-51 0.26434829534398574 1102 52-53 0.3062885125255974 1102 54-55 0.617402225912258 1102 56-57 0.49213652076998216 1102 58-59 0.35664125690750126 1102 60-61 0.38076301519927824 1102 62-63 0.7178980428030499 1102 64-65 0.26498431845783443 1102 66-67 0.3085970904551836 1102 68-69 0.3146285583826547 1102 70-71 0.42810964791861394 1102 72-73 0.4442776494471694 1102 74-75 0.48849179078880667 1102 76-77 0.43652086633379383 1102 78-79 0.34232101013647664 1102 80-81 0.45584892482448325 1102 82-83 0.6284913226360374 1102 84-85 0.728146922281649 1102 86-87 0.7939782157996866 1102 88-89 0.480653392681031 1102 90-91 0.7668615208464225 1102 92-93 0.7099344343926113 1102 94-95 0.6898080536017304 1102 96-97 0.7984136282904792 1102 98-99 1.2410109871967414 1102 100-101 1.6346151193990508 1103 1 0.12890713525211694 1103 2 0.3095145560378647 1103 3 0.29647689390315435 1103 4 0.19726445126280368 1103 5 0.024658022075435326 1103 6 0.29566900684901753 1103 7 0.2133573467256582 1103 8 0.10065567020352262 1103 9 0.1125539748880513 1103 10-11 0.13113905607453802 1103 12-13 0.19845964698771468 1103 14-15 0.16894691983812038 1103 16-17 0.4750845190800774 1103 18-19 0.26563044984524 1103 20-21 0.20649768606124752 1103 22-23 0.18672374969381167 1103 24-25 0.19759025665123886 1103 26-27 0.19262534622715322 1103 28-29 0.009052247845652062 1103 30-31 -0.03238478051504501 1103 32-33 0.2125229531049868 1103 34-35 0.2514013379580078 1103 36-37 0.2763278943232734 1103 38-39 0.024791638952258666 1103 40-41 0.07068994603220347 1103 42-43 0.16919371269288064 1103 44-45 0.03223370968866135 1103 46-47 0.06120387052101961 1103 48-49 0.138302187004804 1103 50-51 0.1733697020718168 1103 52-53 0.4293771975408873 1103 54-55 0.37198938187555797 1103 56-57 0.09611205593817118 1103 58-59 0.6448675566016888 1103 60-61 0.6942186726916333 1103 62-63 0.6315066054941809 1103 64-65 0.5165133704456011 1103 66-67 0.670217885562213 1103 68-69 0.6571362036425903 1103 70-71 0.7560912992947628 1103 72-73 0.7164489032698 1103 74-75 0.5725896501160221 1103 76-77 0.6895789703093307 1103 78-79 0.8797827838367773 1103 80-81 0.866399383540081 1103 82-83 1.1116717507705935 1103 84-85 0.9705016623428087 1103 86-87 0.8352626194694146 1103 88-89 0.6396747994088621 1103 90-91 0.45799301931736736 1103 92-93 1.0677325995302276 1103 94-95 1.1997468915222242 1103 96-97 0.965844820951034 1103 98-99 1.1569131278695224 1103 100-101 1.1751349970748919 1104 1 0.04744580818994848 1104 2 0.25988759003249484 1104 3 0.17837073075262566 1104 4 -0.05546437102535151 1104 5 -0.0972650741295027 1104 6 0.052091708827440186 1104 7 0.11579425908887941 1104 8 -0.007357838321340182 1104 9 0.04533037781033755 1104 10-11 0.15368516690460154 1104 12-13 -0.07068996311542008 1104 14-15 0.013996528574004685 1104 16-17 0.27110989541828445 1104 18-19 0.35564056756629014 1104 20-21 -0.00952933098847808 1104 22-23 0.25783606095502876 1104 24-25 0.09398274219247327 1104 26-27 0.19844645398087835 1104 28-29 0.21709560820514895 1104 30-31 0.18929357622121046 1104 32-33 0.1580866514510575 1104 34-35 0.007943675151565799 1104 36-37 -0.014136153585504019 1104 38-39 0.019470720336109082 1104 40-41 0.031002957330322545 1104 42-43 -0.2609700850073864 1104 44-45 0.0941831028532718 1104 46-47 0.03371397684824018 1104 48-49 -0.1803707052505814 1104 50-51 -0.334847528133686 1104 52-53 -0.1705364372276108 1104 54-55 -0.08802511107622024 1104 56-57 -0.15736306221584329 1104 58-59 -0.06824833687966958 1104 60-61 -0.12625444470583602 1104 62-63 -0.19172951568147312 1104 64-65 0.24178989870002 1104 66-67 -0.05133741780990064 1104 68-69 0.1219942137329646 1104 70-71 0.02066856541361517 1104 72-73 -0.13394782989125886 1104 74-75 -0.008599364408830468 1104 76-77 0.026139036302392782 1104 78-79 0.006934508634767411 1104 80-81 0.17414901096183755 1104 82-83 0.3666930116574001 1104 84-85 0.272392582371797 1104 86-87 0.27277543752817834 1104 88-89 0.34028026753576057 1104 90-91 0.4738839940367363 1104 92-93 0.36574505982272854 1104 94-95 0.1090690953624005 1104 96-97 0.009703090820732285 1104 98-99 0.266423785658116 1104 100-101 1.0264534910840197 1105 1 0.22579783225074834 1105 2 0.2436200260310848 1105 3 0.34063951307926743 1105 4 0.2701746315663982 1105 5 0.1926738522607252 1105 6 0.24714063290240063 1105 7 0.1769645256593364 1105 8 0.17364887595442013 1105 9 0.19373902477250482 1105 10-11 0.221650631205641 1105 12-13 0.04555444821095733 1105 14-15 0.2942911295050479 1105 16-17 0.4007075558741846 1105 18-19 0.17207806770678502 1105 20-21 0.29893960284207566 1105 22-23 0.2914568920686236 1105 24-25 0.3162855117469334 1105 26-27 0.24742560083851828 1105 28-29 0.18067622559483 1105 30-31 0.2822470708140514 1105 32-33 0.46434259415830326 1105 34-35 0.4225271630233891 1105 36-37 0.19841889021351733 1105 38-39 0.04403278636393537 1105 40-41 0.3473584115835564 1105 42-43 0.4369819147768226 1105 44-45 0.45026337744850053 1105 46-47 0.424879545448718 1105 48-49 0.4575315724781248 1105 50-51 0.49307302447346757 1105 52-53 0.44952816547922936 1105 54-55 0.26396635615150643 1105 56-57 0.16671482068556998 1105 58-59 0.5965571244542431 1105 60-61 0.5856801973155967 1105 62-63 0.6854039583661731 1105 64-65 0.34420789214983216 1105 66-67 0.8219814844386057 1105 68-69 1.0762452022173186 1105 70-71 0.7994132858169642 1105 72-73 0.8446055999640016 1105 74-75 0.5429737811855304 1105 76-77 0.8418268842949637 1105 78-79 0.9852631552372806 1105 80-81 1.1228096399503364 1105 82-83 0.9686119862861133 1105 84-85 0.6487572976950453 1105 86-87 0.7225521153665717 1105 88-89 0.840496059458399 1105 90-91 0.8876324121245993 1105 92-93 0.7542907796124219 1105 94-95 0.5278807562184191 1105 96-97 0.8681480852348678 1105 98-99 0.8617161499958037 1105 100-101 0.8955779378362365 1106 1 0.0491305098033763 1106 2 0.1011279236659739 1106 3 0.007894121069391247 1106 4 0.082390695480143 1106 5 0.007854497232358426 1106 6 -0.07585623970201993 1106 7 0.04649582335890301 1106 8 -0.07107039337019216 1106 9 0.1138423215404103 1106 10-11 0.030792440391550713 1106 12-13 -0.06464045433776988 1106 14-15 0.12819092169041113 1106 16-17 0.1882187776388733 1106 18-19 0.10623881538723623 1106 20-21 -0.025260288162648692 1106 22-23 -0.019307409038745504 1106 24-25 0.03767594775064964 1106 26-27 -0.10067410496468909 1106 28-29 -0.0716195722076165 1106 30-31 0.022223236577325167 1106 32-33 -0.11399154194106842 1106 34-35 0.031833369648580856 1106 36-37 -0.0036983769447616055 1106 38-39 0.3622628116228057 1106 40-41 0.3628947610558839 1106 42-43 0.3280420408223961 1106 44-45 0.011410405744307184 1106 46-47 -0.08971722856247055 1106 48-49 -0.008384790607301795 1106 50-51 0.4060904976373436 1106 52-53 0.36702190729609896 1106 54-55 0.34666030329557884 1106 56-57 0.4246186741886788 1106 58-59 0.43307551684175394 1106 60-61 0.2714312132103629 1106 62-63 0.2194695047784876 1106 64-65 0.43961833172642883 1106 66-67 0.3842026363061102 1106 68-69 0.45795253859438034 1106 70-71 0.6831931153592059 1106 72-73 0.7736840471657409 1106 74-75 0.5811255197958651 1106 76-77 0.006874451009348803 1106 78-79 0.14608885098668978 1106 80-81 0.32966774935717424 1106 82-83 0.8251429967989559 1106 84-85 0.5332844911119139 1106 86-87 0.4644560502769437 1106 88-89 0.5030561821120045 1106 90-91 0.5059793472543177 1106 92-93 0.7661242294033634 1106 94-95 0.8734410077668535 1106 96-97 0.6147623346712372 1106 98-99 0.9359731391482846 1106 100-101 1.151812942505849 1107 1 -0.14088559325620764 1107 2 0.027933751521278083 1107 3 -0.007190217103403285 1107 4 -0.002910670930667436 1107 5 0.07418885142278242 1107 6 -0.09834285963991363 1107 7 -0.1755576798040437 1107 8 -0.17602432665996304 1107 9 0.01911190896891668 1107 10-11 0.003953295861251149 1107 12-13 -0.04917489651838736 1107 14-15 0.08568546501703622 1107 16-17 -0.02681045987912256 1107 18-19 -0.20227252723204714 1107 20-21 -0.1944406552440654 1107 22-23 -0.2831199472141037 1107 24-25 -0.19100458377845797 1107 26-27 0.07288227923637436 1107 28-29 -0.0253318186485032 1107 30-31 -0.2925392539722722 1107 32-33 -0.11140736394460049 1107 34-35 -0.0582839648465594 1107 36-37 0.22648994769633646 1107 38-39 0.1480632874532617 1107 40-41 0.35435522740390724 1107 42-43 -0.13793010131166739 1107 44-45 0.1561457303896745 1107 46-47 0.5734885617047922 1107 48-49 0.4127016747070442 1107 50-51 0.4328249936372046 1107 52-53 0.05556972740314592 1107 54-55 0.2469142361582044 1107 56-57 0.1796610248082473 1107 58-59 0.04896201803560274 1107 60-61 0.12661366471964897 1107 62-63 0.11761444940019317 1107 64-65 0.3012633639111897 1107 66-67 0.012529498516030912 1107 68-69 0.15685522400381302 1107 70-71 0.22383091611694894 1107 72-73 0.28766744008466816 1107 74-75 0.37023203491436973 1107 76-77 0.16372230913156827 1107 78-79 0.26270575620929293 1107 80-81 0.34574411288481244 1107 82-83 -0.03888759474287795 1107 84-85 0.30214258343327316 1107 86-87 0.2879534895914766 1107 88-89 0.37581531536589097 1107 90-91 0.2640778620994588 1107 92-93 0.38635044108384875 1107 94-95 0.5211353230023974 1107 96-97 0.6064587941338111 1107 98-99 1.2661488632520452 1107 100-101 1.9037478724767603 1108 1 -0.030376265612463982 1108 2 0.05876358146484506 1108 3 0.015241998090999687 1108 4 -0.1247760313484676 1108 5 -0.011108694731483126 1108 6 0.02699853675022723 1108 7 -0.09124697437074047 1108 8 -0.02324497077868415 1108 9 -0.1186965050581037 1108 10-11 -0.24187735469613614 1108 12-13 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-1.7050291878884885 2316 82-83 -1.6422416896700085 2316 84-85 -1.4592625740392968 2316 86-87 -1.7565799004475302 2316 88-89 -1.440043295436542 2316 90-91 -1.7413789158292268 2316 92-93 -1.9526580327560872 2316 94-95 -1.9863838951909791 2316 96-97 -1.7023447618607541 2316 98-99 -1.9276805075436165 2316 100-101 -2.087708548754943 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 8.0 5 20.0 6 62.0 7 133.0 8 293.0 9 538.0 10 934.0 11 1572.0 12 2385.0 13 3180.0 14 4172.0 15 5737.0 16 7515.0 17 9977.0 18 8140.0 19 5137.0 20 5070.0 21 5522.0 22 6052.0 23 6878.0 24 7476.0 25 8486.0 26 10189.0 27 12113.0 28 14994.0 29 18047.0 30 21773.0 31 26428.0 32 32330.0 33 40570.0 34 52563.0 35 70496.0 36 96156.0 37 110713.0 38 54425.0 39 2514.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.17692035311412 20.53062156730797 14.99738644045453 15.295071639123378 2 13.526584854540328 28.965354485159807 32.86695926148414 24.641101398815728 3 30.504846186262114 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18.14729144846577 27.8238803660182 30.04954868328607 23.979279502229957 24-25 24.471994201628263 28.150144729442523 28.809663544270048 18.56819752465917 26-27 22.63968211888055 22.514178533830034 37.58181102132948 17.264328325959934 28-29 16.693075315218124 34.39969106478445 31.96391465051704 16.94331896948039 30-31 19.05912260781222 27.878478622970214 30.112566331542016 22.94983243767555 32-33 19.720471315824355 29.157744003395656 31.719349646068757 19.402435034711235 34-35 24.38134060747234 26.15609771048518 30.69696876819812 18.765592913844362 36-37 21.828389802910106 24.411760874579226 35.97463370826466 17.78521561424601 38-39 19.439195764276793 29.268939306867725 34.363377238351376 16.928487690504106 40-41 18.819435292819644 31.13252506183093 31.78362698556206 18.264412659787368 42-43 16.554320660376927 32.9435317661589 26.845204620922253 23.65694295254192 44-45 20.58629281388678 25.832832765341895 28.75533258145978 24.825541839311548 46-47 24.421966867963732 30.981794388914512 28.379285351999155 16.216953391122598 48-49 22.300822712945354 29.12701100372818 29.287753391449854 19.28441289187661 50-51 14.69363487796039 30.740864746998714 35.83820299097986 18.727297384061032 52-53 19.742042057227618 35.04390985416504 25.644779929725846 19.5692681588815 54-55 16.482117983459577 28.605403450595556 29.635923706256524 25.276554859688343 56-57 22.136138346459166 27.204760697126158 27.935077502304267 22.724023454110405 58-59 27.68932315549702 26.802555032259693 25.74163588568399 19.766485926559298 60-61 20.739273047403856 29.51299738650129 33.534689962126876 16.21303960396798 62-63 19.027373182770358 29.453869528773026 31.779433105568266 19.739324182888353 64-65 17.731740900429013 35.947969831942494 28.183365272406707 18.136923995221782 66-67 20.816662401564244 28.22399602809145 25.91639684147457 25.042944728869738 68-69 17.904416609713415 29.980753376587156 28.376050825796607 23.73877918790282 70-71 24.266895085971367 29.655792743192244 26.04776885110504 20.029543319731353 72-73 22.029359710080524 24.226281231085128 33.6876623326872 20.056696726147152 74-75 17.328328441074138 36.25459056349478 27.820213114013008 18.59686788141807 76-77 19.817204449368436 27.909228961042743 27.62535876442528 24.64820782516354 78-79 19.726394747024322 28.91044427332943 29.991073925518997 21.372087054127256 80-81 23.75084377856492 28.08409206361612 28.021493463158386 20.143570694660575 82-83 20.726200053633683 26.143661648086425 32.922499329578976 20.207638968700916 84-85 18.606092693717212 29.538201736675916 31.00050033061663 20.855205238990237 86-87 19.187718315643334 30.802819264720192 28.662013863402503 21.34744855623397 88-89 18.075318103592274 30.64575450662719 25.637318229255264 25.64160916052527 90-91 20.39171513298545 24.340359991724327 28.85588837038229 26.41203650490793 92-93 23.434266507404686 30.081359989028396 28.701098003581095 17.783275499985827 94-95 21.286942477269264 27.906228379269844 29.11227724183268 21.694551901628216 96-97 14.155924952037708 28.50875579991296 34.627932135655136 22.70738711239419 98-99 19.45623733397693 31.96308512716783 25.38460654400018 23.196070994855056 100-101 17.158581819379883 27.090565971235204 28.487047719894537 27.263804489490372 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1510.0 1 1093.5 2 907.5 3 1110.0 4 929.5 5 920.0 6 1455.0 7 2367.0 8 3164.0 9 3411.0 10 2855.0 11 2143.5 12 1945.5 13 1862.0 14 1695.0 15 1518.5 16 1395.0 17 1338.5 18 1302.5 19 1252.5 20 1292.0 21 1426.0 22 1575.0 23 1710.0 24 1804.5 25 2200.0 26 3103.0 27 3945.0 28 4258.5 29 5001.0 30 6295.5 31 7370.0 32 9575.5 33 12666.5 34 15795.0 35 17809.0 36 20164.5 37 22512.5 38 21213.5 39 18840.0 40 16695.0 41 14498.0 42 12303.0 43 11225.5 44 11082.0 45 10849.0 46 13514.0 47 47533.5 48 123321.0 49 115175.0 50 39679.5 51 10879.0 52 3656.0 53 1764.5 54 1191.0 55 918.0 56 803.5 57 728.5 58 697.0 59 612.5 60 490.0 61 415.5 62 378.5 63 342.0 64 286.0 65 288.0 66 264.0 67 187.5 68 126.5 69 118.5 70 110.5 71 74.5 72 60.0 73 72.5 74 68.5 75 49.0 76 34.5 77 25.5 78 23.5 79 23.0 80 15.5 81 9.5 82 10.0 83 9.5 84 5.0 85 2.0 86 1.5 87 2.0 88 3.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03585651876108066 2 0.007508416321764754 3 0.003984057640120073 4 4.596989584753931E-4 5 9.193979169507862E-4 6 0.0 7 0.0 8 0.0 9 0.0015323298615846435 10-11 2.2984947923769655E-4 12-13 9.960144100300183E-4 14-15 0.002298494792376965 16-17 0.0027581937508523585 18-19 0.0 20-21 7.661649307923219E-5 22-23 0.0016089463546638757 24-25 6.129319446338575E-4 26-27 0.00459698958475393 28-29 0.0056696204878631815 30-31 0.001225863889267715 32-33 3.830824653961609E-4 34-35 0.009347212155666326 36-37 0.012488488371914845 38-39 0.008581047224874004 40-41 0.002298494792376965 42-43 6.129319446338575E-4 44-45 0.0013790968754261793 46-47 0.0 48-49 6.129319446338575E-4 50-51 8.427814238715541E-4 52-53 0.002145261806218501 54-55 0.0027581937508523585 56-57 0.0011492473961884826 58-59 9.960144100300183E-4 60-61 0.00421390711935777 62-63 0.004290523612437002 64-65 0.008657663717953236 66-67 0.0027581937508523585 68-69 0.0029114267370108225 70-71 0.0 72-73 9.193979169507862E-4 74-75 0.006052702953259342 76-77 0.002145261806218501 78-79 0.0028348102439315907 80-81 0.004673606077833163 82-83 0.003984057640120073 84-85 0.004980072050150091 86-87 0.002988043230090055 88-89 0.00950044514182479 90-91 0.013178036809627936 92-93 0.0011492473961884826 94-95 0.0023751112854561974 96-97 7.661649307923217E-4 98-99 0.0028348102439315907 100-101 0.004980072050150091 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 652601.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.90681289179759 #Duplication Level Percentage of deduplicated Percentage of total 1 72.14178676891886 10.032623302753137 2 9.807616025386752 2.727853619592983 3 4.3082551015910795 1.797422927638787 4 2.4417118427431794 1.358257189308628 5 1.6417647318083655 1.1415857468805595 6 1.190003966679889 0.9929497503068491 7 0.8803825642381771 0.8570320915842912 8 0.7569747454713738 0.8421684919269201 9 0.5905945612411301 0.7391959252284321 >10 5.053109436290713 14.480363958988724 >50 0.6743355811186037 6.5461131686895975 >100 0.43082551015910797 11.066639493350454 >500 0.02754638811759002 2.5767658952407366 >1k 0.034157521265811626 10.783464934929613 >5k 0.01212041077173961 11.356709536148427 >10k+ 0.008814844197628808 22.70085396743186 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 45830 7.022667755642422 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 19785 3.0317146311452174 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 19573 2.999229238079623 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13986 2.1431165444122824 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 12714 1.948204186018716 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 12707 1.9471315551156065 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 12656 1.9393166728215248 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 10895 1.6694733841964693 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9626 1.475020724761378 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 9375 1.4365592452356035 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9187 1.407751443837812 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7259 1.112318246524293 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6301 0.9655210457844839 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6191 0.9486654173070529 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5602 0.8584111884597173 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5229 0.8012552846226102 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5151 0.78930311170225 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5145 0.788383713785299 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5048 0.7735201141279281 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4788 0.7336795377267274 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4379 0.6710072463879154 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4373 0.6700878484709646 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4142 0.6346910286683594 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4004 0.6135448765784913 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3940 0.6037379654643495 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3701 0.5671152817724766 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3486 0.5341701897484068 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3048 0.4670541418109994 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2659 0.4074465101953567 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2164 0.3315961820469169 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2045 0.3133614566940596 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2010 0.3079983021785134 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1924 0.29482026536888545 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1883 0.2885377129363884 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1843 0.2824083934900498 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1632 0.2500762334106138 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1583 0.24256781708884909 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1566 0.2399628563241552 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1565 0.23980962333799674 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1527 0.2339867698639751 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1524 0.2335270709054997 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1392 0.21330031673258237 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1264 0.19368649450429895 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1263 0.1935332615181405 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1255 0.19230739762887278 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1131 0.17330650734522318 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1092 0.1673304208850431 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1077 0.1650319260926661 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1059 0.16227373234181375 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1054 0.16150756741102143 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 986 0.15108772435224585 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 969 0.14848276358755197 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 823 0.12611074760841617 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 804 0.12319932087140534 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 781 0.11967496218976068 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 766 0.1173764673973837 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 745 0.11415857468805594 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 741 0.1135456427434221 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 728 0.11155361392336206 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 712 0.10910188614482663 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 709 0.10864218718635123 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 698 0.10695662433860811 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 660 0.10113377086458648 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 659 0.100980537878428 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 7.661649307923217E-5 0.0 18-19 0.0 0.0 0.0 1.5323298615846435E-4 0.0 20-21 0.0 0.0 0.0 1.5323298615846435E-4 0.0 22-23 0.0 0.0 0.0 8.42781423871554E-4 0.0 24-25 0.0 0.0 0.0 0.001685562847743108 0.0 26-27 0.0 0.0 0.0 0.0024517277785354296 0.0 28-29 0.0 0.0 0.0 0.004673606077833163 0.0 30-31 0.0 0.0 0.0 0.008197964759477844 0.0 32-33 0.0 0.0 0.0 0.011952172920360219 0.0 34-35 0.0 0.0 0.0 0.01647254601203492 0.0 36-37 0.0 0.0 0.0 0.020839686117551152 0.0 38-39 0.0 0.0 0.0 0.02597299115385971 0.0 40-41 0.0 0.0 0.0 0.03593313525415989 0.0 42-43 0.0 0.0 0.0 0.04895793907762937 0.0 44-45 0.0 0.0 0.0 0.07477769724533061 0.0 46-47 0.0 0.0 0.0 0.13660720716027097 0.0 48-49 0.0 0.0 0.0 0.1797422927638787 0.0 50-51 0.0 0.0 0.0 0.23084549364772655 0.0 52-53 0.0 0.0 0.0 0.284783504775506 0.0 54-55 0.0 0.0 0.0 0.34691948066276335 0.0 56-57 0.0 0.0 0.0 0.42744341488903637 0.0 58-59 0.0 0.0 0.0 0.522371249814205 0.0 60-61 0.0 0.0 0.0 0.6464133521094819 0.0 62-63 0.0 0.0 0.0 0.7861618354860014 0.0 64-65 0.0 0.0 0.0 0.8737344870755638 0.0 66-67 0.0 0.0 0.0 1.0505653531024317 0.0 68-69 0.0 0.0 0.0 1.2784228035200682 0.0 70-71 0.0 0.0 0.0 1.4175583549519537 0.0 72-73 0.0 0.0 0.0 1.6210517605703945 0.0 74-75 0.0 0.0 0.0 1.8236257682718844 0.0 76-77 0.0 0.0 0.0 1.9375544934807025 0.0 78-79 0.0 0.0 0.0 2.1569075131665443 0.0 80-81 0.0 0.0 0.0 2.3859142109803697 0.0 82-83 0.0 0.0 0.0 2.6159169232042245 0.0 84-85 0.0 0.0 0.0 2.9957048793979784 0.0 86-87 0.0 0.0 0.0 3.4944016328507006 0.0 88-89 0.0 0.0 0.0 4.092929676785662 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGTATT 25 0.00501257 57.02221 1 TATAACG 25 0.005018656 57.004734 2 GACATAT 145 0.0 49.157078 1 GCAAGCG 20 5.388279E-4 47.500305 94-95 AGGGTCT 60 1.4920079E-6 47.496666 5 GGATGTA 65 2.5888967E-6 43.843075 8 TAGGCAT 150 0.0 41.163776 5 GCTTTGC 145 0.0 39.325665 1 TTAGGCA 160 0.0 38.59104 4 CATTCCG 150 0.0 38.000244 9 TAGGGTC 75 6.92102E-6 37.997334 4 TGGGGAC 50 0.0016158615 37.997334 6 TGGGAGT 75 6.92102E-6 37.997334 6 CATATAG 190 0.0 37.497368 3 CTTATGT 140 1.0913936E-11 37.324528 2 GGGATAC 65 1.328104E-4 36.535896 7 CTTTAGG 145 1.6370905E-11 36.037476 2 GCTTATG 160 1.8189894E-12 35.63888 1 ACATATA 200 0.0 35.627956 2 GGCATTC 160 1.8189894E-12 35.622498 7 >>END_MODULE