##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936648_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 665931 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.58151820533959 31.0 31.0 34.0 30.0 34.0 2 31.532395998984878 31.0 31.0 34.0 30.0 34.0 3 31.497117569237655 31.0 31.0 34.0 28.0 34.0 4 35.08537370988886 37.0 35.0 37.0 32.0 37.0 5 35.18956017965825 37.0 35.0 37.0 33.0 37.0 6 35.35362072046503 37.0 35.0 37.0 33.0 37.0 7 35.27597754121673 37.0 35.0 37.0 33.0 37.0 8 35.29215639458142 37.0 35.0 37.0 33.0 37.0 9 36.71226898882917 39.0 37.0 39.0 32.0 39.0 10-11 36.580507590125706 39.0 37.0 39.0 32.0 39.0 12-13 36.57895487670645 39.0 37.0 39.0 32.0 39.0 14-15 37.29896190446158 39.0 37.0 41.0 32.0 41.0 16-17 37.37254610462645 39.0 37.0 41.0 32.0 41.0 18-19 37.48676664699496 40.0 37.0 41.0 32.0 41.0 20-21 37.46257720394455 39.5 37.0 41.0 32.0 41.0 22-23 37.47518812009052 39.0 37.0 41.0 32.0 41.0 24-25 37.50344104719558 39.5 37.0 41.0 32.0 41.0 26-27 37.33380710614163 39.0 36.5 41.0 31.5 41.0 28-29 37.320865825438375 39.0 36.0 41.0 32.0 41.0 30-31 37.236257960659586 39.0 36.0 41.0 32.0 41.0 32-33 36.95332324219777 39.0 35.0 41.0 31.0 41.0 34-35 36.570733304201184 38.5 35.0 40.0 30.0 41.0 36-37 36.23045555770793 38.0 35.0 40.0 30.0 41.0 38-39 35.95530242622735 38.0 35.0 40.0 30.0 41.0 40-41 35.993362675712646 38.0 35.0 40.0 30.0 41.0 42-43 35.64904472084946 38.0 35.0 40.0 29.0 41.0 44-45 35.30666991024596 38.0 35.0 40.0 27.0 41.0 46-47 34.87607124461844 38.0 34.5 40.0 24.5 41.0 48-49 34.412792015989645 38.0 33.5 40.0 23.0 41.0 50-51 33.269404037355216 37.0 32.0 39.0 22.5 40.5 52-53 33.506034408970294 37.5 33.0 39.5 23.0 40.0 54-55 34.072111825399325 38.0 34.0 40.0 22.5 41.0 56-57 33.65370511359285 38.0 33.0 40.0 20.5 41.0 58-59 33.416725606707004 38.0 33.0 40.0 19.0 41.0 60-61 32.7351136979657 37.0 31.0 40.0 13.0 41.0 62-63 32.56262360514828 36.0 31.5 39.0 13.0 41.0 64-65 32.19801826315339 36.0 31.0 39.0 8.0 40.5 66-67 31.43125413894232 36.0 30.0 39.0 5.0 40.0 68-69 30.855722289546513 35.0 29.5 38.0 2.0 39.5 70-71 30.098062712202918 35.0 29.0 37.0 2.0 39.0 72-73 29.57881597342668 34.0 28.5 37.0 2.0 39.0 74-75 29.22314999602061 34.0 29.0 36.0 2.0 38.0 76-77 28.775678711458095 34.0 28.5 35.5 2.0 37.0 78-79 28.164270021969244 34.0 26.5 35.0 2.0 37.0 80-81 27.375243080739594 33.0 26.0 35.0 2.0 36.0 82-83 26.882857232956567 33.0 25.0 35.0 2.0 36.0 84-85 26.410620619854008 32.0 24.5 35.0 2.0 35.5 86-87 26.416064877592422 33.0 25.0 35.0 2.0 35.0 88-89 25.83635241488983 32.5 21.5 35.0 2.0 35.0 90-91 25.192647586611827 32.0 19.5 35.0 2.0 35.0 92-93 24.679555389372172 32.0 16.5 35.0 2.0 35.0 94-95 24.12241283256073 31.0 4.5 34.0 2.0 35.0 96-97 23.397861790485802 31.0 2.0 34.0 2.0 35.0 98-99 22.8016949203446 31.0 2.0 34.0 2.0 35.0 100-101 20.422214914157774 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.547710735391167 1101 2 0.563215065274548 1101 3 0.7082954951926297 1101 4 0.4905410597438973 1101 5 0.41873257461800506 1101 6 0.4833010526785557 1101 7 0.48211583987666984 1101 8 0.429193255487597 1101 9 0.7276492595133348 1101 10-11 0.7412670532528836 1101 12-13 0.7580767743955832 1101 14-15 0.9946150963298734 1101 16-17 1.1117189303673314 1101 18-19 1.0765671881039722 1101 20-21 0.9746372345609089 1101 22-23 0.9897067137970623 1101 24-25 0.8955847968100343 1101 26-27 0.9181395252557607 1101 28-29 0.9766824536877081 1101 30-31 0.9026884724311159 1101 32-33 0.7302308188233226 1101 34-35 0.8642162444450392 1101 36-37 1.0433573420613769 1101 38-39 1.1907211337765062 1101 40-41 1.2118475832215054 1101 42-43 1.314061528817497 1101 44-45 1.4983279175062236 1101 46-47 1.5963679223368743 1101 48-49 1.810247108440116 1101 50-51 1.8188732267233334 1101 52-53 1.7963253934089352 1101 54-55 1.8658922084770495 1101 56-57 2.21814065692287 1101 58-59 2.005890999118499 1101 60-61 1.7555059961957156 1101 62-63 1.8570224093777554 1101 64-65 1.654618784157627 1101 66-67 1.666984137824386 1101 68-69 1.8873107716893678 1101 70-71 1.9986519903504067 1101 72-73 2.0062536418481187 1101 74-75 1.924284150480343 1101 76-77 2.024260689369836 1101 78-79 1.9137024205680788 1101 80-81 1.7998832232018742 1101 82-83 1.8249751243427603 1101 84-85 1.8756982877558812 1101 86-87 1.9079653761477608 1101 88-89 1.9542260374586604 1101 90-91 1.9509821845112434 1101 92-93 2.0551680370970686 1101 94-95 2.134950057491846 1101 96-97 2.3755799950015586 1101 98-99 2.1727594648115343 1101 100-101 2.971258993774523 1102 1 0.4829159395930951 1102 2 0.526037762544064 1102 3 0.5379442688356164 1102 4 0.4444100860560454 1102 5 0.5330565390515005 1102 6 0.44992046916266304 1102 7 0.3906079863235945 1102 8 0.4497566303915477 1102 9 0.33564456313155233 1102 10-11 0.6114925177603538 1102 12-13 0.598764908872468 1102 14-15 0.8093299486372629 1102 16-17 0.4929026190315824 1102 18-19 0.5427284209996444 1102 20-21 0.7436411148031752 1102 22-23 0.6307150995112778 1102 24-25 0.6458222409092542 1102 26-27 0.6068431520652311 1102 28-29 0.3375795866993556 1102 30-31 0.4650587134070534 1102 32-33 0.7176021336670502 1102 34-35 0.5435831874536845 1102 36-37 0.4124015520049156 1102 38-39 0.30005161300790206 1102 40-41 0.7182740853239054 1102 42-43 0.4777344907357559 1102 44-45 1.0112680243202732 1102 46-47 1.0439595948501577 1102 48-49 0.9034218434334136 1102 50-51 0.995869308299099 1102 52-53 0.9257014046344381 1102 54-55 1.216381176275334 1102 56-57 1.094569497670662 1102 58-59 0.772306857997954 1102 60-61 1.0629137748166784 1102 62-63 1.1555636196506143 1102 64-65 0.9398488066372579 1102 66-67 0.8124598135258623 1102 68-69 1.312811790670576 1102 70-71 0.9890921806875035 1102 72-73 1.5134545219424282 1102 74-75 1.2949677985762555 1102 76-77 1.3386187789351816 1102 78-79 1.1974015848603088 1102 80-81 1.1823507804628903 1102 82-83 1.3030753806392035 1102 84-85 1.5945036217764894 1102 86-87 1.4095881096901408 1102 88-89 1.5916858867353554 1102 90-91 1.916174550027204 1102 92-93 1.8403300479502889 1102 94-95 1.5486946105958026 1102 96-97 1.7538666270180947 1102 98-99 1.9367185528352806 1102 100-101 2.2927535313670546 1103 1 0.20741449716527072 1103 2 0.2190323335637565 1103 3 0.2746568963257907 1103 4 0.3187371423675174 1103 5 0.296764146438548 1103 6 0.1891794928548194 1103 7 0.4673633187634607 1103 8 0.3081465629268152 1103 9 0.2602794130963417 1103 10-11 0.17009029619435978 1103 12-13 0.2731214952023393 1103 14-15 0.779495543894285 1103 16-17 0.7619131362612066 1103 18-19 0.6779236598520626 1103 20-21 0.5810193034772695 1103 22-23 0.5219401409668762 1103 24-25 0.5541292622390088 1103 26-27 0.28007873346585654 1103 28-29 0.3888289336316433 1103 30-31 0.616079770581706 1103 32-33 0.5687049662772736 1103 34-35 0.1588343061870674 1103 36-37 0.16751251672790346 1103 38-39 0.13801472320030683 1103 40-41 0.1670531326400777 1103 42-43 0.31329658327829435 1103 44-45 0.5053086394532684 1103 46-47 0.6880081603727177 1103 48-49 0.6652542946520938 1103 50-51 0.6708040015276353 1103 52-53 0.7051769060768578 1103 54-55 0.6393305436514751 1103 56-57 0.8251807690515349 1103 58-59 0.9400498559762909 1103 60-61 0.6909286761182685 1103 62-63 0.610265707195758 1103 64-65 0.708718488394517 1103 66-67 0.8401112684471457 1103 68-69 1.2017164541419483 1103 70-71 1.276146674929148 1103 72-73 1.1133080076203683 1103 74-75 1.1290579332785704 1103 76-77 1.2985340504977714 1103 78-79 1.4996958401359564 1103 80-81 1.512286268730385 1103 82-83 1.2188603112436631 1103 84-85 1.4329278545638964 1103 86-87 1.4527087511730024 1103 88-89 1.596899070752798 1103 90-91 1.4753345345807318 1103 92-93 1.3758251300664597 1103 94-95 1.4013625341811995 1103 96-97 1.2330922590740627 1103 98-99 1.6162497070046946 1103 100-101 1.8897210256314132 1104 1 0.20072643283901925 1104 2 0.23978510174713819 1104 3 0.37778102866550256 1104 4 0.5273310051633544 1104 5 0.30015815583426786 1104 6 0.17523251314558053 1104 7 0.10731622910430616 1104 8 0.1678460331681464 1104 9 0.41010671399259735 1104 10-11 0.4295687560071073 1104 12-13 0.4676506541410461 1104 14-15 0.4131713138213158 1104 16-17 0.30229696140753504 1104 18-19 0.4659546679483739 1104 20-21 0.5461263102852811 1104 22-23 0.5876179988316323 1104 24-25 0.5219239335554136 1104 26-27 0.2685984078364214 1104 28-29 0.1912045180876092 1104 30-31 0.015494481657775339 1104 32-33 0.02619433223278378 1104 34-35 -0.25595895466076257 1104 36-37 0.2458490523061414 1104 38-39 -0.0651960363425772 1104 40-41 0.04868816556952282 1104 42-43 0.20039912545352223 1104 44-45 0.357922448717936 1104 46-47 0.5919086057253935 1104 48-49 0.43827103147199153 1104 50-51 0.03303009852789529 1104 52-53 0.13139718350603147 1104 54-55 0.2744412154565481 1104 56-57 0.3652969383521807 1104 58-59 0.35981862798015385 1104 60-61 0.4036073447247972 1104 62-63 0.2595288206662971 1104 64-65 0.4464835065529158 1104 66-67 0.2931704303844853 1104 68-69 0.4023361718261107 1104 70-71 0.4410215652568894 1104 72-73 0.6471514789278814 1104 74-75 0.591643866353504 1104 76-77 0.5952188940690348 1104 78-79 0.6200432374327463 1104 80-81 0.7126838653808036 1104 82-83 0.7616941313922609 1104 84-85 0.9988767000898235 1104 86-87 0.7779428112123163 1104 88-89 0.9045866797309294 1104 90-91 1.2296264682553897 1104 92-93 1.4245562636611524 1104 94-95 1.3457711924590932 1104 96-97 1.3571535200551708 1104 98-99 1.2187585640809147 1104 100-101 1.4514195851765095 1105 1 0.180532882648631 1105 2 0.04667567773203629 1105 3 0.30837000004579096 1105 4 0.21718413320706276 1105 5 0.2509793788427004 1105 6 0.3219152624097177 1105 7 0.20528891878972644 1105 8 0.13084960875097806 1105 9 0.13770879440988892 1105 10-11 0.14360705753000502 1105 12-13 0.2063234958371183 1105 14-15 0.3787152087770167 1105 16-17 0.5631597024583641 1105 18-19 0.2679204859785145 1105 20-21 0.22855736259887038 1105 22-23 0.4716254897099148 1105 24-25 0.1364113867642942 1105 26-27 0.23083225482383796 1105 28-29 0.40745847692218007 1105 30-31 -0.06224595639950081 1105 32-33 0.32414487609548104 1105 34-35 -0.001516352118080988 1105 36-37 0.07116903794361207 1105 38-39 -0.22802373539159504 1105 40-41 0.020773678546326835 1105 42-43 0.02078145265698339 1105 44-45 0.06217788680746139 1105 46-47 0.419411997476395 1105 48-49 0.5688582827883764 1105 50-51 0.5292459579470759 1105 52-53 0.4772687076331579 1105 54-55 0.4781634337369596 1105 56-57 0.3764289769074125 1105 58-59 0.5550469557125268 1105 60-61 0.3573085997264158 1105 62-63 0.5097228653750534 1105 64-65 0.33259915074909685 1105 66-67 0.22425284509619559 1105 68-69 0.5302014220201592 1105 70-71 0.4157205003223261 1105 72-73 0.38563511359491187 1105 74-75 0.24899423691977063 1105 76-77 0.5892696230536174 1105 78-79 0.2620022612104762 1105 80-81 0.3689968224071549 1105 82-83 0.19217022357908675 1105 84-85 0.012166343800089408 1105 86-87 0.5065704797302004 1105 88-89 0.445964966934298 1105 90-91 0.45064070393424416 1105 92-93 0.3088432649232473 1105 94-95 0.4264744405847658 1105 96-97 0.3657667400362925 1105 98-99 0.7982932437942658 1105 100-101 1.6190924892248972 1106 1 0.22611390054096603 1106 2 0.08673699344354446 1106 3 0.028417403789138262 1106 4 0.3981170079607068 1106 5 0.3908977104370379 1106 6 0.25853207785715426 1106 7 0.3442347007582711 1106 8 0.4651297288061116 1106 9 0.4910599041469794 1106 10-11 0.5844425485065585 1106 12-13 0.4712595394279546 1106 14-15 0.3899896996376242 1106 16-17 0.49672361378245 1106 18-19 0.5744281955275383 1106 20-21 0.3853342944946405 1106 22-23 0.2040358670076472 1106 24-25 0.21140075956640203 1106 26-27 0.17922593775977447 1106 28-29 0.3829547348603768 1106 30-31 0.2555523458251301 1106 32-33 0.2312374035651814 1106 34-35 0.45300890196855903 1106 36-37 0.2215857529157219 1106 38-39 0.33393926907826454 1106 40-41 0.4395174793475505 1106 42-43 0.24119597778225454 1106 44-45 0.2293565911910207 1106 46-47 0.23136212049426064 1106 48-49 0.04988068363339693 1106 50-51 -0.020450683858392438 1106 52-53 0.13200392938735206 1106 54-55 0.47089507444749046 1106 56-57 0.5592715269196518 1106 58-59 0.6684649201854782 1106 60-61 0.5885177816253986 1106 62-63 0.5223434679693781 1106 64-65 0.3873775253674765 1106 66-67 0.18817833877784196 1106 68-69 0.408655261336925 1106 70-71 0.6903015310961145 1106 72-73 0.6461689209656782 1106 74-75 0.9659211089167847 1106 76-77 0.8241145947788304 1106 78-79 0.6727756635883964 1106 80-81 1.1889946325603162 1106 82-83 0.8824518121197471 1106 84-85 1.1465359899465994 1106 86-87 0.9429232254116968 1106 88-89 0.9648266330213033 1106 90-91 1.316844282401611 1106 92-93 1.2836278226385929 1106 94-95 1.572237731022863 1106 96-97 1.6761678684514898 1106 98-99 2.037363331774582 1106 100-101 2.7223180048050146 1107 1 -0.036008667487934076 1107 2 0.1754148629208565 1107 3 0.1553107782215406 1107 4 0.12108359839523075 1107 5 0.08000314280587162 1107 6 0.007674466243351219 1107 7 0.2155681003804233 1107 8 0.2374276307649481 1107 9 0.2612572791096923 1107 10-11 0.2731854638888791 1107 12-13 0.1258512831553702 1107 14-15 0.04013802087325047 1107 16-17 0.05896053002651769 1107 18-19 0.18797265875381441 1107 20-21 0.14220140198195708 1107 22-23 0.11233149857437752 1107 24-25 0.20561084541041907 1107 26-27 0.12209740373703681 1107 28-29 0.1430762091237412 1107 30-31 0.03174885903317204 1107 32-33 0.27011579672417696 1107 34-35 0.11596132316772412 1107 36-37 0.3634500431281751 1107 38-39 0.30977699404595427 1107 40-41 0.24093481718852416 1107 42-43 -0.006610276567954543 1107 44-45 0.4325463263671807 1107 46-47 0.09995903474967349 1107 48-49 0.40175002836840434 1107 50-51 0.2913180675916749 1107 52-53 0.44234954272466354 1107 54-55 0.7847643528936814 1107 56-57 0.7691129309168687 1107 58-59 0.9345047431943314 1107 60-61 0.6647374959205834 1107 62-63 0.5440675573267058 1107 64-65 0.7876563527182512 1107 66-67 0.7335323210787266 1107 68-69 0.6670159388086709 1107 70-71 0.6859844716681884 1107 72-73 0.7028872929122514 1107 74-75 0.8031592680762394 1107 76-77 0.6207736382311495 1107 78-79 1.060830418154037 1107 80-81 1.0060188445519636 1107 82-83 0.8699522264248749 1107 84-85 0.6701617398388819 1107 86-87 1.0159838631101472 1107 88-89 1.187583999366467 1107 90-91 1.1961696279320861 1107 92-93 1.2339215649739508 1107 94-95 1.1197191701531501 1107 96-97 1.2443873507358028 1107 98-99 1.7587742064555698 1107 100-101 1.8888065202668791 1108 1 -0.020373282142152505 1108 2 0.10133668599412715 1108 3 0.21769406671655744 1108 4 0.3019310426110877 1108 5 0.3032663173588048 1108 6 0.2227599228029291 1108 7 0.3366315597885574 1108 8 0.2557691404974989 1108 9 -0.10926416415664875 1108 10-11 0.20512683119382302 1108 12-13 0.16024952654831282 1108 14-15 0.5974453479819672 1108 16-17 0.5242714969516697 1108 18-19 0.4617105919710909 1108 20-21 0.48829453380661647 1108 22-23 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-1.3861712151855912 2315 82-83 -1.5148192484803893 2315 84-85 -1.6200835399016498 2315 86-87 -1.4662200140389956 2315 88-89 -1.683505549044117 2315 90-91 -2.009302315275871 2315 92-93 -1.811966513992143 2315 94-95 -2.1629348071101724 2315 96-97 -1.840072611866173 2315 98-99 -2.2775182179623634 2315 100-101 -2.470026280778942 2316 1 -4.569463155753962 2316 2 -4.985619264326772 2316 3 -5.66494015228329 2316 4 -7.44212301736307 2316 5 -7.67764621850959 2316 6 -8.680477542423887 2316 7 -11.083110844872692 2316 8 -11.028085151662925 2316 9 -10.19196628668967 2316 10-11 -11.089328078669709 2316 12-13 -9.312585774527193 2316 14-15 -8.881723600389442 2316 16-17 -8.045950613314908 2316 18-19 -8.33736066293001 2316 20-21 -9.589056352224972 2316 22-23 -9.589932074564175 2316 24-25 -8.81838678682611 2316 26-27 -7.224847657160787 2316 28-29 -5.5828639065559145 2316 30-31 -7.929783721741636 2316 32-33 -5.742317145768006 2316 34-35 -3.8735748660436613 2316 36-37 -4.794092506717178 2316 38-39 -3.5758757572777213 2316 40-41 -3.052240825427724 2316 42-43 -3.048355213398466 2316 44-45 -3.4123728114523786 2316 46-47 -2.87027245563074 2316 48-49 -3.7418622525479464 2316 50-51 -3.684949690634337 2316 52-53 -3.8623613018067644 2316 54-55 -3.0399512533760813 2316 56-57 -3.6945820906638502 2316 58-59 -3.978306298796337 2316 60-61 -4.1415798892297815 2316 62-63 -4.0498074929118175 2316 64-65 -4.8622084401242915 2316 66-67 -3.937346372544912 2316 68-69 -4.052045867681272 2316 70-71 -4.074377769931058 2316 72-73 -3.377498713693022 2316 74-75 -3.1623825653240765 2316 76-77 -3.7531538785004592 2316 78-79 -3.711353166517533 2316 80-81 -4.428709299505911 2316 82-83 -3.9143650538798767 2316 84-85 -3.69450113782376 2316 86-87 -4.541852691313466 2316 88-89 -5.799381287997889 2316 90-91 -5.460956815879403 2316 92-93 -4.674693468139793 2316 94-95 -4.305094510452154 2316 96-97 -5.250807225251059 2316 98-99 -6.8667423766258455 2316 100-101 -7.478985343713408 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1120.0 3 364.0 4 288.0 5 290.0 6 319.0 7 482.0 8 596.0 9 815.0 10 1162.0 11 1862.0 12 2824.0 13 3830.0 14 4528.0 15 5986.0 16 8159.0 17 10692.0 18 8133.0 19 5973.0 20 6190.0 21 6849.0 22 7831.0 23 8569.0 24 9581.0 25 10366.0 26 11793.0 27 13386.0 28 15451.0 29 18395.0 30 21912.0 31 26398.0 32 32381.0 33 40713.0 34 51852.0 35 69599.0 36 84817.0 37 95314.0 38 67754.0 39 9356.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.23106159087596 19.35371885177042 14.47249578077778 15.94272377657584 2 13.379218559181878 27.05931481601096 33.80330139293005 25.75816523187711 3 29.038948789693357 26.868251772618244 30.519672645733177 13.57312679195522 4 10.074017110448908 16.368475439776986 45.27194679419399 28.28556065558012 5 8.936049410902362 43.726155214159974 34.852290000660894 12.48550537427677 6 23.63888175376772 40.236074872206224 16.121918942019253 20.003124432006803 7 22.267874532623203 37.71370090289383 22.280798778508483 17.737625785974487 8 22.859718580733375 24.232557790115017 21.01449166485114 31.893231964300472 9 20.912790365675164 24.547151268156778 27.737454429607457 26.802603936560605 10-11 24.68139359639257 24.250599723071815 26.21261262885688 24.855394051678736 12-13 32.69713383475138 24.911533241041965 24.108177701193803 18.28315522301285 14-15 24.910231370192307 26.269080528846157 33.37627704326923 15.444411057692308 16-17 17.029932525786478 30.289017427895903 31.733991872441354 20.94705817387626 18-19 15.84344517966933 35.32978442084513 30.499687479340583 18.32708292014496 20-21 20.85896665665365 26.889005707419646 27.212000600781018 25.04002703514569 22-23 17.76067980703957 27.476435638805953 30.326762145633456 24.436122408521015 24-25 24.581161662435978 27.446115024708234 28.43451942863151 19.53820388422428 26-27 21.9908102814295 22.670500973347114 37.649982575885026 17.688706169338364 28-29 16.50834412719866 33.85361641418408 31.81445814796043 17.82358131065683 30-31 18.290314127673824 27.936467176785325 30.226670180583803 23.54654851495705 32-33 19.747377621721228 28.582327547042862 31.83355848228332 19.836736348952588 34-35 24.25772499911766 26.038704406378994 29.937755456816674 19.765815137686673 36-37 21.211932359568618 24.420655545435636 36.36232577242799 18.00508632256776 38-39 19.764456582382753 29.327963872529622 33.47032316634991 17.437256378737718 40-41 18.290918401595434 31.409525282643152 32.1147346867367 18.184821629024714 42-43 16.372097702940234 32.3543567399216 26.568646369327087 24.704899187811087 44-45 19.761835935593588 26.494025250496488 28.47179310060029 25.27234571330963 46-47 24.06846090602956 30.476440753685647 28.700646842186273 16.754451498098515 48-49 21.53526528552808 28.831067809539828 29.516956889975432 20.116710014956663 50-51 14.370891059967624 30.58402885605908 36.18705381679045 18.85802626718284 52-53 18.916102293033802 34.66137582028141 26.0401994203595 20.382322466325288 54-55 15.857546375064008 28.210364768616476 30.399562115480776 25.53252674083874 56-57 21.912753844744554 26.915464425319374 27.684938933097463 23.48684279683861 58-59 26.91250419544662 26.66028933752115 26.409125687696584 20.01808077933565 60-61 20.904847790404133 28.69561234496337 33.92124236549225 16.478297499140247 62-63 18.642520278626034 29.844551182178385 31.799768887861056 19.71315965133453 64-65 17.299807110844814 35.46427421044214 28.681768933958928 18.554149744754113 66-67 20.146907663510422 28.512220155767476 25.73887436864468 25.60199781207742 68-69 17.263876666536518 29.710678147835566 29.121101903883755 23.90434328174416 70-71 24.10637457558142 28.803473737481433 26.19013651974513 20.900015167192013 72-73 21.207287463379064 24.2538190540777 34.28958947750249 20.249304005040752 74-75 16.938608302662143 35.55557725047443 28.25396873077966 19.25184571608377 76-77 18.989252641777256 27.656685367520595 28.40562077071551 24.94844121998663 78-79 19.587223173899623 28.165086698603975 30.883537966921683 21.36415216057472 80-81 23.537371646899626 27.65000247807653 28.152976920847234 20.65964895417661 82-83 20.177745874158745 25.75903540275524 33.845076059345736 20.218142663740277 84-85 18.908301271365886 29.19230873196796 31.049500073584845 20.849889923081314 86-87 18.946284047846625 30.885865333572603 29.71139905048583 20.45645156809494 88-89 18.09973743033119 29.817762984509365 25.99206960010872 26.090429985050722 90-91 19.77563609195316 24.71051574414405 29.314030628526805 26.199817535375985 92-93 23.469166311859425 29.43818807261947 29.675627332998427 17.417018282522683 94-95 20.90986983944136 27.35526931363265 30.09252340870025 21.642337438225738 96-97 14.222410456621107 28.366423288600128 35.502109001233784 21.90905725354498 98-99 19.254501185846742 31.376273151798895 26.5275944058675 22.84163125648686 100-101 17.28123871169298 26.40020066432508 30.181131094934806 26.137429529047136 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 961.0 1 720.5 2 738.0 3 980.5 4 843.0 5 832.5 6 1337.5 7 2384.0 8 3568.0 9 3863.5 10 3003.5 11 2192.0 12 1990.0 13 1959.5 14 1849.5 15 1636.5 16 1458.0 17 1386.5 18 1350.5 19 1335.0 20 1370.0 21 1486.5 22 1649.5 23 1771.5 24 1858.5 25 2272.0 26 3206.5 27 4029.5 28 4276.0 29 4907.5 30 6315.5 31 7645.0 32 9969.5 33 13135.0 34 16448.5 35 18627.0 36 21011.0 37 22969.5 38 21370.5 39 18987.0 40 16687.0 41 14799.5 42 12753.0 43 11622.5 44 11781.0 45 11996.0 46 16277.0 47 48558.5 48 119285.5 49 113662.5 50 42376.0 51 12628.5 52 4589.0 53 2305.5 54 1481.5 55 1176.5 56 977.5 57 809.0 58 710.0 59 604.5 60 509.0 61 439.5 62 402.0 63 378.0 64 343.5 65 318.5 66 275.5 67 199.0 68 124.5 69 87.0 70 81.5 71 64.0 72 53.5 73 94.5 74 96.0 75 53.5 76 30.0 77 20.5 78 19.5 79 14.0 80 9.5 81 6.5 82 4.0 83 2.5 84 3.0 85 2.5 86 1.0 87 0.5 88 0.5 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07868683091791792 2 0.03183513006602786 3 0.016217896448731176 4 0.020873033392348455 5 0.02507767321238987 6 0.03153479865031062 7 0.07733533954719032 8 0.05646230615484187 9 0.0716290426485627 10-11 0.062393851615257435 12-13 0.042872309593636575 14-15 0.04970484930120388 16-17 0.06269418303097468 18-19 0.05631214044698325 20-21 0.01967170772947948 22-23 0.01426574224656909 24-25 0.02417667896523814 26-27 0.02808098736956231 28-29 0.0065322082918500566 30-31 0.001126242808939665 32-33 0.011337510943325961 34-35 0.01343983085334667 36-37 0.004129556966112105 38-39 0.009986019572598362 40-41 0.004429888381829349 42-43 0.002177402763950019 44-45 5.255799775051769E-4 46-47 5.255799775051769E-4 48-49 0.0010511599550103539 50-51 7.5082853929311E-4 52-53 1.50165707858622E-4 54-55 3.00331415717244E-4 56-57 0.003303645572889684 58-59 0.004580054089687971 60-61 0.00600662831434488 62-63 0.0030783970111017507 64-65 6.75745685363799E-4 66-67 3.75414269646555E-4 68-69 0.004354805527900038 70-71 0.00300331415717244 72-73 0.023651098987732964 74-75 0.019896956291267413 76-77 0.02793082166170369 78-79 0.022149441909146743 80-81 0.013815245122993222 82-83 0.0054810483368397025 84-85 0.004955468359334525 86-87 0.0017269056403741527 88-89 0.0018770713482327748 90-91 0.007283036831143166 92-93 0.011487676651184582 94-95 0.014941487931932888 96-97 0.014190659392639778 98-99 0.024326844673096763 100-101 0.021398613369853634 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 665931.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.254987126725517 #Duplication Level Percentage of deduplicated Percentage of total 1 73.85634607323122 11.266776085741267 2 9.433364810471991 2.878117174909114 3 3.951582533055395 1.8084402201586036 4 2.327572332011756 1.4202834384544731 5 1.4890246304310555 1.1357525784301485 6 1.11926301159619 1.0244605699991944 7 0.795475988743668 0.8494483177532736 8 0.7295194201254629 0.890304749016816 9 0.5296510848443333 0.7271838432860858 >10 4.686912429253429 14.838818984947046 >50 0.6095984183028352 6.440379169450317 >100 0.400736009408913 11.118900126020423 >500 0.023984200064373847 2.527001967353322 >1k 0.02798156674176949 9.859515277305064 >5k 0.010992758362838013 10.988250953359842 >10k+ 0.007994733354791282 22.226366543815008 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 39470 5.92704048917981 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 26205 3.9350923744351896 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 20081 3.015477579508988 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 15307 2.298586490191927 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 12123 1.8204588763700742 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 11800 1.7719553527317393 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 10479 1.5735864526505 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10330 1.5512117621795651 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9607 1.4426419553977814 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8220 1.2343621185978728 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7991 1.1999741714982481 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6814 1.02322913334865 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6542 0.982384060811105 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6076 0.9124068409489872 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5578 0.8376243184353935 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5570 0.8364229927725244 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5386 0.8087925025265381 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5152 0.7736537268876205 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5142 0.7721520698090343 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4853 0.7287541802378925 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4355 0.6539716577242988 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4201 0.6308461387140709 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4111 0.617331225006795 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3643 0.5470536737289599 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3495 0.5248291489658838 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3372 0.5063587668992733 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3326 0.49945114433777676 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3271 0.4911920304055525 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2860 0.4294739244756589 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2268 0.3405758254233547 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2044 0.3069387068630233 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1972 0.29612677589720254 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1891 0.2839633535606542 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1853 0.27825705666202655 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1725 0.25903584605612295 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1643 0.24672225801171593 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1593 0.23921397261878483 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1587 0.2383129783716331 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1363 0.20467585981130176 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1360 0.20422536268772593 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1341 0.2013722142384121 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1182 0.17749586668889117 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1116 0.16758492997022215 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1091 0.16383078727375658 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1073 0.16112780453230138 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1058 0.15887531891442208 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1027 0.15422018197080478 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 947 0.14220692534211501 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 911 0.13680095985920462 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 908 0.13635046273562879 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 885 0.13289665145488047 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 864 0.1297431715898494 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 821 0.12328604615192865 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 789 0.11848074350045276 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 741 0.11127278952323888 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 727 0.10917046961321818 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 719 0.1079691439503492 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 717 0.10766881253463197 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.50165707858622E-4 0.0 12-13 0.0 0.0 0.0 1.50165707858622E-4 0.0 14-15 0.0 0.0 0.0 3.00331415717244E-4 0.0 16-17 0.0 0.0 0.0 3.00331415717244E-4 0.0 18-19 0.0 0.0 0.0 3.00331415717244E-4 0.0 20-21 0.0 0.0 0.0 3.00331415717244E-4 0.0 22-23 0.0 0.0 0.0 5.255799775051769E-4 0.0 24-25 0.0 0.0 0.0 0.001201325662868976 0.0 26-27 0.0 0.0 0.0 0.001877071348232775 0.0 28-29 0.0 0.0 0.0 0.004129556966112105 0.0 30-31 0.0 0.0 0.0 0.0075082853929311 0.0 32-33 0.0 0.0 0.0 0.012613919460124247 0.0 34-35 0.0 0.0 0.0 0.016443145010519106 0.0 36-37 0.0 0.0 0.0 0.020797950538419145 0.0 38-39 0.0 0.0 0.0 0.026879661706693335 0.0 40-41 0.0 0.0 0.0 0.03603976988606927 0.0 42-43 0.0 0.0 0.0 0.05068092640228492 0.0 44-45 0.0 0.0 0.0 0.07658451100789722 0.0 46-47 0.0 0.0 0.0 0.13124482866843562 0.0 48-49 0.0 0.0 0.0 0.17096365839704114 0.0 50-51 0.0 0.0 0.0 0.21879143635001225 0.0 52-53 0.0 0.0 0.0 0.2716497655162472 0.0 54-55 0.0 0.0 0.0 0.3362210198954546 0.0 56-57 0.0 0.0 0.0 0.41625934218410016 0.0 58-59 0.0 0.0 0.0 0.5173208635729527 0.0 60-61 0.0 0.0 0.0 0.6403065783091642 0.0 62-63 0.0 0.0 0.0 0.7853666521005931 0.0 64-65 0.0 0.0 0.0 0.8819232022536869 0.0 66-67 0.0 0.0 0.0 1.059419068942578 0.0 68-69 0.0 0.0 0.0 1.285568624977663 0.0 70-71 0.0 0.0 0.0 1.4257483132636866 0.0 72-73 0.0 0.0 0.0 1.6383829555914953 0.0 74-75 0.0 0.0 0.0 1.8379531813356038 0.0 76-77 0.0 0.0 0.0 1.9663448615547257 0.0 78-79 0.0 0.0 0.0 2.193395411836962 0.0 80-81 0.0 0.0 0.0 2.415265245198076 0.0 82-83 0.0 0.0 0.0 2.6489981694800213 7.5082853929311E-5 84-85 0.0 0.0 0.0 3.0336476301598814 1.50165707858622E-4 86-87 0.0 0.0 0.0 3.5143280610153305 1.50165707858622E-4 88-89 0.0 0.0 0.0 4.117844040899132 1.50165707858622E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTAATC 20 0.0020675717 71.30311 8 GAGCGCA 25 0.0050040763 57.046772 9 GGCATCT 25 0.0050055655 57.042484 8 ATGTATT 45 1.5119647E-5 52.79727 1 GTATGGT 45 1.5119647E-5 52.79727 1 ATGGGCG 55 8.1477447E-7 51.852913 5 CTTATGT 120 0.0 51.47734 2 GCTTATG 180 0.0 42.237816 1 GGGACAT 50 0.0016100195 38.025463 7 GGGCATC 50 0.0016100195 38.025463 7 CATGGGT 235 0.0 36.40462 4 TACCTGG 105 4.6373316E-8 36.203846 2 CTTAGGG 80 1.0725453E-5 35.646194 3 ATGGGTA 135 3.0559022E-10 35.208767 5 ATGACTC 55 0.0025694415 34.571205 8 GGGAGCT 110 6.964001E-8 34.568607 7 GTAAGCG 195 0.0 34.110035 94-95 CATGGAT 85 1.6245473E-5 33.549362 4 GACATAT 130 8.245479E-9 32.896763 1 GCTTTGC 175 3.6379788E-12 32.58346 1 >>END_MODULE