##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936648_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 665931 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74523036170414 31.0 31.0 34.0 30.0 34.0 2 31.802808399068372 31.0 31.0 34.0 30.0 34.0 3 31.906298099953297 31.0 31.0 34.0 30.0 34.0 4 35.521545024935016 37.0 35.0 37.0 33.0 37.0 5 35.38832551720824 37.0 35.0 37.0 33.0 37.0 6 35.608803314457504 37.0 35.0 37.0 33.0 37.0 7 35.570150661254694 37.0 35.0 37.0 33.0 37.0 8 35.606061288632006 37.0 35.0 37.0 33.0 37.0 9 37.11183591092771 39.0 37.0 39.0 33.0 39.0 10-11 36.98105509429656 39.0 37.0 39.0 32.5 39.0 12-13 36.9476582408688 39.0 37.0 39.0 33.0 39.0 14-15 37.68515957358946 39.0 36.5 41.0 32.0 41.0 16-17 37.77732452761622 39.0 37.0 41.0 32.5 41.0 18-19 38.17054920104335 40.0 37.0 41.0 33.0 41.0 20-21 38.24132830578543 40.0 37.5 41.0 33.5 41.0 22-23 38.1344238367038 40.0 37.0 41.0 33.0 41.0 24-25 37.86535316722002 39.5 37.0 41.0 32.5 41.0 26-27 37.53823894667766 39.0 36.5 41.0 32.5 41.0 28-29 37.52762823776037 39.0 36.5 41.0 32.5 41.0 30-31 37.58641360741578 39.0 36.0 41.0 33.0 41.0 32-33 37.34278476298596 39.0 35.5 41.0 32.0 41.0 34-35 36.97984776200537 39.0 35.0 41.0 31.0 41.0 36-37 36.65979358221797 38.5 35.0 40.5 30.5 41.0 38-39 36.43443464863476 38.0 35.0 40.0 30.0 41.0 40-41 36.49241137595337 39.0 35.0 40.0 31.0 41.0 42-43 36.12676313311739 38.0 35.0 40.0 30.0 41.0 44-45 35.74270757781211 38.0 35.0 40.0 29.5 41.0 46-47 35.249338895471155 38.0 35.0 40.0 27.0 41.0 48-49 34.70044268850677 38.0 34.0 40.0 24.0 41.0 50-51 34.411081628577136 38.0 34.0 40.0 23.0 41.0 52-53 34.24580925050793 38.0 33.5 40.0 23.0 41.0 54-55 34.204288432285026 38.0 33.5 40.0 23.0 41.0 56-57 33.753761275567584 38.0 33.0 40.0 22.5 41.0 58-59 33.42640528823557 38.0 33.0 40.0 20.5 41.0 60-61 32.68088510671526 36.5 31.0 39.0 18.5 40.5 62-63 32.53242528129791 36.0 31.0 39.0 18.0 40.0 64-65 32.148724867891715 36.0 31.0 39.0 15.0 40.0 66-67 31.502735268368646 35.5 30.5 38.5 10.5 40.0 68-69 30.98705346349697 35.0 30.0 37.0 7.5 39.0 70-71 30.375147725515106 34.5 29.5 37.0 4.5 39.0 72-73 29.77873082947032 34.0 29.0 36.0 2.0 38.5 74-75 29.323145340883663 34.0 29.0 36.0 2.0 37.0 76-77 27.781180032165494 31.5 26.5 34.0 2.0 36.0 78-79 28.566465594783843 33.5 29.0 35.0 2.0 36.0 80-81 28.115682405534507 33.0 27.0 35.0 2.0 36.0 82-83 27.73687138757619 33.0 26.5 35.0 2.0 36.0 84-85 27.300577687478132 33.0 26.0 35.0 2.0 35.0 86-87 27.222948773972078 33.0 26.0 35.0 2.0 35.0 88-89 26.66751209960191 32.5 25.0 34.5 2.0 35.0 90-91 26.002278013788214 32.0 24.5 34.0 2.0 35.0 92-93 25.445749634721913 32.0 21.5 34.0 2.0 35.0 94-95 24.826557856594754 31.0 19.0 34.0 2.0 35.0 96-97 24.00715689763654 31.0 6.5 34.0 2.0 35.0 98-99 23.31998660521886 31.0 2.0 34.0 2.0 35.0 100-101 20.31047736176871 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29599170527523455 1101 2 0.24654245398329877 1101 3 0.28032646101197756 1101 4 0.2807288474671523 1101 5 0.2814316110602064 1101 6 0.24431690834069286 1101 7 0.194583720060713 1101 8 0.1884057606790961 1101 9 0.34877490779613396 1101 10-11 0.24805194582589252 1101 12-13 0.30729501836533757 1101 14-15 0.42464283327951335 1101 16-17 0.5440306704289597 1101 18-19 0.4938056478037467 1101 20-21 0.1853452991172233 1101 22-23 0.3167485078436556 1101 24-25 0.42764604691512176 1101 26-27 0.43463337742272046 1101 28-29 0.1806141417289311 1101 30-31 0.38426445235068485 1101 32-33 0.2675503930881433 1101 34-35 0.32128854671116613 1101 36-37 0.33476305758997427 1101 38-39 0.4981808477303318 1101 40-41 0.5966969329156484 1101 42-43 0.5136111783219306 1101 44-45 0.7075132729064535 1101 46-47 1.0249984721438068 1101 48-49 0.9553108148937355 1101 50-51 0.7087048715105766 1101 52-53 0.9247114611366456 1101 54-55 1.0164597695103907 1101 56-57 1.120063223318958 1101 58-59 1.0205854795530058 1101 60-61 1.0575941080556674 1101 62-63 1.3904516333976673 1101 64-65 1.269079291823715 1101 66-67 1.210691904966584 1101 68-69 1.422869014344741 1101 70-71 1.2374098648450875 1101 72-73 1.2259753626516847 1101 74-75 1.121628245086562 1101 76-77 0.9364091115515194 1101 78-79 1.137330682845441 1101 80-81 1.3173168998844638 1101 82-83 1.166311324408536 1101 84-85 1.1855115965376903 1101 86-87 1.321362775500365 1101 88-89 1.3744685612390626 1101 90-91 1.694912280360171 1101 92-93 1.8481968464239742 1101 94-95 1.594616689170632 1101 96-97 1.9636225066024515 1101 98-99 2.1033108724905496 1101 100-101 3.3442181954264925 1102 1 0.34822702072986544 1102 2 0.21665313395638108 1102 3 0.19614715850103792 1102 4 0.22798762096695668 1102 5 0.11318516556257663 1102 6 0.19946264413103876 1102 7 0.43161974951809867 1102 8 0.4238500794412374 1102 9 0.423110422274263 1102 10-11 0.40898296533211465 1102 12-13 0.2732808528560611 1102 14-15 0.5348729750777892 1102 16-17 0.5277508348023119 1102 18-19 0.2829863105472157 1102 20-21 0.38654636057587055 1102 22-23 0.3813058595425147 1102 24-25 0.2970875879392665 1102 26-27 0.3386546543231148 1102 28-29 0.6541707441067146 1102 30-31 0.6182709814668215 1102 32-33 0.4514526377104531 1102 34-35 0.5648274422023505 1102 36-37 0.5712311303075239 1102 38-39 0.5031051281196 1102 40-41 0.5278262122787751 1102 42-43 0.7542864263853843 1102 44-45 0.745456601323859 1102 46-47 0.6877216576652074 1102 48-49 1.0191331637528123 1102 50-51 0.8233163938732773 1102 52-53 1.0425338481776834 1102 54-55 1.125068370952711 1102 56-57 0.9619612488340579 1102 58-59 1.1323393204201224 1102 60-61 1.2352619535905234 1102 62-63 1.368289388679905 1102 64-65 0.9613612847314172 1102 66-67 1.0498766245439164 1102 68-69 1.2184809519363213 1102 70-71 1.3874852742273553 1102 72-73 1.3700502374864278 1102 74-75 1.4101132801423795 1102 76-77 1.1296730968896433 1102 78-79 1.3981325995801193 1102 80-81 1.408130318936312 1102 82-83 1.6200204637393014 1102 84-85 1.6476088364588435 1102 86-87 1.566709348430308 1102 88-89 1.569562894558075 1102 90-91 1.3051245243620215 1102 92-93 1.5467072724869837 1102 94-95 1.5492887299489695 1102 96-97 1.5438845780695445 1102 98-99 1.745974207780705 1102 100-101 2.491606548972406 1103 1 0.006417057755960798 1103 2 0.2510964931095536 1103 3 0.06781651315655424 1103 4 -0.03714878500264973 1103 5 0.22972651894603757 1103 6 0.14168829890240886 1103 7 0.0165889701527675 1103 8 -0.049984052716951055 1103 9 -0.1883926556622697 1103 10-11 0.025218796602132443 1103 12-13 0.05714951242038069 1103 14-15 0.15939084073892218 1103 16-17 0.05119312633174644 1103 18-19 0.053826553147672485 1103 20-21 0.055075311351608036 1103 22-23 0.006019077633055758 1103 24-25 0.17004944878473083 1103 26-27 -0.15324389870598765 1103 28-29 -0.04672197106490472 1103 30-31 0.03682832649101897 1103 32-33 0.1064295815807057 1103 34-35 -0.01590989964169154 1103 36-37 0.02422842717506768 1103 38-39 0.12433153249913431 1103 40-41 -0.22422385246010634 1103 42-43 -0.058052578225840534 1103 44-45 0.018313971334983137 1103 46-47 0.16148423631727837 1103 48-49 0.17734932348749766 1103 50-51 0.178470517853917 1103 52-53 0.410993516987169 1103 54-55 0.2813684868012487 1103 56-57 0.23145901136727787 1103 58-59 0.34168443547361704 1103 60-61 0.49556225116224795 1103 62-63 0.6272426631046386 1103 64-65 0.6766396619433266 1103 66-67 0.420273916868382 1103 68-69 0.8271854927445332 1103 70-71 0.5116487796676914 1103 72-73 0.33163168354871786 1103 74-75 0.26219173912972593 1103 76-77 0.3998057548184555 1103 78-79 0.4808529957366723 1103 80-81 0.5439070719972072 1103 82-83 0.435555524957298 1103 84-85 0.7665625651915526 1103 86-87 1.0390408569599359 1103 88-89 0.8723103220391657 1103 90-91 0.7978283382774762 1103 92-93 0.9197418226085112 1103 94-95 1.1092017299716836 1103 96-97 0.8840669827216594 1103 98-99 0.8350287928839499 1103 100-101 1.315661020498652 1104 1 0.02397572547400273 1104 2 0.1317553040956554 1104 3 0.03706385603369 1104 4 0.19020542030381904 1104 5 0.12426345212092116 1104 6 0.05160489245423605 1104 7 0.11555277553031829 1104 8 0.022818337305366754 1104 9 -0.024818283987144696 1104 10-11 0.024173717138324946 1104 12-13 0.15343342752572653 1104 14-15 0.15528937585069968 1104 16-17 0.24332782052660917 1104 18-19 0.08733462169422523 1104 20-21 -0.0597479358357873 1104 22-23 0.06324300895323631 1104 24-25 0.020275456936566627 1104 26-27 0.11625777275479265 1104 28-29 0.1970536422668019 1104 30-31 0.16066857704353765 1104 32-33 0.22811192629033172 1104 34-35 0.11142764658725213 1104 36-37 0.4253168202404467 1104 38-39 0.5423633180190635 1104 40-41 0.5232967215006354 1104 42-43 0.40564951304369146 1104 44-45 0.5922080862382018 1104 46-47 0.3084906000848946 1104 48-49 0.6137693987865234 1104 50-51 0.604538642374969 1104 52-53 0.7310543781504109 1104 54-55 0.617975143768632 1104 56-57 0.47463489790785474 1104 58-59 0.616198268060991 1104 60-61 0.7995137843526834 1104 62-63 0.6186876714897096 1104 64-65 0.5748038315993753 1104 66-67 0.423689027762304 1104 68-69 0.5056930615187625 1104 70-71 0.6339308511684543 1104 72-73 0.8202102199932249 1104 74-75 0.6769683409000287 1104 76-77 0.7399996664809194 1104 78-79 0.6832807786355737 1104 80-81 1.0668566172043938 1104 82-83 0.8211343168410004 1104 84-85 1.050151152612841 1104 86-87 1.0393862551674218 1104 88-89 0.7640018847716981 1104 90-91 0.9554054420413145 1104 92-93 0.8234279211636135 1104 94-95 0.871608855614447 1104 96-97 1.072106875659319 1104 98-99 1.0725716900085907 1104 100-101 2.015212336010471 1105 1 -0.041191045471538956 1105 2 0.059749129066254625 1105 3 0.1192442090338055 1105 4 0.13002898474923796 1105 5 0.20124921214191005 1105 6 0.07549442995055244 1105 7 0.08669655352166927 1105 8 0.22467858641362426 1105 9 0.03628913615351337 1105 10-11 0.16221959554270882 1105 12-13 0.160382485683229 1105 14-15 0.11086012530404332 1105 16-17 0.21029721843974158 1105 18-19 0.1707684712617379 1105 20-21 0.20304020174352644 1105 22-23 0.24513761633789954 1105 24-25 0.0059623752055841805 1105 26-27 -0.04456390177041669 1105 28-29 0.06691145748462901 1105 30-31 0.15757998730243372 1105 32-33 0.07832876184577486 1105 34-35 0.1731446143726103 1105 36-37 0.12245871906198857 1105 38-39 0.07219245117135387 1105 40-41 0.12907205105413055 1105 42-43 0.17707344644281875 1105 44-45 0.2674137951302882 1105 46-47 0.41251683965969477 1105 48-49 0.2555929675321096 1105 50-51 0.496017660820506 1105 52-53 0.5576439859543498 1105 54-55 0.474650819269975 1105 56-57 0.20354315184949456 1105 58-59 0.4090799986172797 1105 60-61 0.4373446547695181 1105 62-63 0.3714908381273503 1105 64-65 0.8196839538959182 1105 66-67 0.6305922892184732 1105 68-69 0.5744794919346532 1105 70-71 0.6141802174324091 1105 72-73 0.7535007667370373 1105 74-75 0.7343754735750103 1105 76-77 0.4595883992023424 1105 78-79 0.5772818407750329 1105 80-81 0.6777996637385719 1105 82-83 0.901325845956304 1105 84-85 0.8621191422236514 1105 86-87 0.999029255904901 1105 88-89 0.7266109206973823 1105 90-91 1.0029415580080219 1105 92-93 1.0082305115798462 1105 94-95 1.0039484220293424 1105 96-97 1.0623379877451349 1105 98-99 0.9048934107605184 1105 100-101 1.50199256989616 1106 1 0.1496734287719974 1106 2 0.28670099108014213 1106 3 0.24214614253974887 1106 4 0.31891615611119306 1106 5 0.2164300033206885 1106 6 0.10815535148962852 1106 7 -0.08014401274696326 1106 8 0.020410961366927438 1106 9 0.14022313159347988 1106 10-11 0.18860402959612088 1106 12-13 0.19750038961336713 1106 14-15 0.4468051730298299 1106 16-17 0.1894646901971626 1106 18-19 0.44755867105305924 1106 20-21 0.1567764668953302 1106 22-23 0.2679571085510588 1106 24-25 0.2918339986549796 1106 26-27 0.20600932284346385 1106 28-29 0.03740649168488375 1106 30-31 0.1388954411803951 1106 32-33 -0.03160799022492 1106 34-35 0.06554262547641798 1106 36-37 0.046103912348179676 1106 38-39 0.2938371549623682 1106 40-41 0.32413265828577664 1106 42-43 0.20719350157543914 1106 44-45 0.4036190481864068 1106 46-47 0.4691050582836951 1106 48-49 0.35734355685853814 1106 50-51 0.7113890974888548 1106 52-53 0.46923536053409265 1106 54-55 0.41023711855762457 1106 56-57 0.13204787290164433 1106 58-59 0.36165693308933555 1106 60-61 0.6482880923137984 1106 62-63 0.7902475203805679 1106 64-65 0.9291891773246945 1106 66-67 0.9695065116296213 1106 68-69 0.937810506606624 1106 70-71 0.8941714580450508 1106 72-73 0.8664743211160832 1106 74-75 0.9047616595058869 1106 76-77 0.43901994072570005 1106 78-79 0.21310065027124026 1106 80-81 0.5805318197071934 1106 82-83 0.6314723080655149 1106 84-85 0.9883992365158818 1106 86-87 0.8177453615843326 1106 88-89 0.534770676465051 1106 90-91 0.49975017827570056 1106 92-93 0.684410130546496 1106 94-95 1.080003734984217 1106 96-97 1.3270904706450075 1106 98-99 1.7710680059608883 1106 100-101 2.046394934930749 1107 1 0.06443429185243943 1107 2 0.0023468099127939013 1107 3 -0.005629038925896168 1107 4 0.1836123875917579 1107 5 -0.041309216459893605 1107 6 -0.05381309503187026 1107 7 -0.03597080005928177 1107 8 0.13921295666042255 1107 9 0.14011541226034296 1107 10-11 0.019161518575600667 1107 12-13 -0.08784605233419995 1107 14-15 0.04667963857620805 1107 16-17 0.23606365940600682 1107 18-19 0.0292119970946203 1107 20-21 -0.045528726833744315 1107 22-23 -0.017704205293355813 1107 24-25 -0.1418253928136508 1107 26-27 0.0162637890527364 1107 28-29 -0.002281867998249254 1107 30-31 -3.9394266502768005E-4 1107 32-33 0.1141321244547413 1107 34-35 0.3173945693960789 1107 36-37 0.28104392096572894 1107 38-39 0.18137195659307537 1107 40-41 0.188797816881781 1107 42-43 0.32649059149621706 1107 44-45 0.49639646689974626 1107 46-47 0.33922247235681624 1107 48-49 0.6243238525066772 1107 50-51 0.41501799610009726 1107 52-53 0.7023109960781468 1107 54-55 0.7238412417222619 1107 56-57 0.2069521684172102 1107 58-59 0.511190011210708 1107 60-61 0.6371743572702115 1107 62-63 0.635001174551796 1107 64-65 0.5468041884280765 1107 66-67 0.5104149999130705 1107 68-69 0.5740174603038852 1107 70-71 0.7132982685584572 1107 72-73 0.9232258374728524 1107 74-75 1.068169816345236 1107 76-77 0.6968130201728506 1107 78-79 0.6132323993017685 1107 80-81 0.9067618916305626 1107 82-83 0.9304328165007654 1107 84-85 1.0018745107858749 1107 86-87 0.8026750125736939 1107 88-89 0.7560572596078075 1107 90-91 1.081529867381132 1107 92-93 1.1122029614105742 1107 94-95 1.1001348211265345 1107 96-97 1.5450853995502492 1107 98-99 1.7008267146546636 1107 100-101 1.7813121567662868 1108 1 -0.012136368062105163 1108 2 -0.02774497676231391 1108 3 0.07581185362039022 1108 4 0.03636598784495959 1108 5 0.14557953175975769 1108 6 0.14915414662029036 1108 7 0.07397539395876152 1108 8 0.018786465126950702 1108 9 0.13805021207043922 1108 10-11 0.09102415506872319 1108 12-13 0.08858020181993709 1108 14-15 0.19384493371784828 1108 16-17 0.12835080927148823 1108 18-19 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2316 94-95 -2.311001967452281 2316 96-97 -2.310290996206998 2316 98-99 -2.9604564209281854 2316 100-101 -3.9977289864004 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 5.0 5 20.0 6 53.0 7 139.0 8 279.0 9 546.0 10 955.0 11 1683.0 12 2440.0 13 3257.0 14 4197.0 15 5915.0 16 7717.0 17 10144.0 18 8309.0 19 5371.0 20 4989.0 21 5684.0 22 6296.0 23 6993.0 24 7851.0 25 9002.0 26 10534.0 27 12770.0 28 15253.0 29 18634.0 30 22283.0 31 27079.0 32 33263.0 33 41250.0 34 53478.0 35 71917.0 36 97617.0 37 111620.0 38 55800.0 39 2586.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.18117982801998 20.53411437798055 14.954751980774287 15.329953813225188 2 13.486939427115951 28.896207755696025 32.91282388613806 24.70402893104997 3 30.49733580829834 26.43842323103078 29.789688046279444 13.27455291439144 4 10.43411404484849 16.951455931620544 44.2320600782964 28.382369945234565 5 8.875466847273552 44.645090951685155 34.46477940480821 12.014662796233084 6 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37.549855838539166 17.27669990389236 28-29 16.702870284926107 34.31937823382907 32.02169363854998 16.95605784269484 30-31 18.999701165753898 27.87943314597377 30.07729145048737 23.043574237784963 32-33 19.666475935595976 29.19529510001367 31.717600873458196 19.420628090932166 34-35 24.435944854782406 26.09614297330964 30.657163945221193 18.810748226686762 36-37 21.64719485824123 24.397525228255645 36.14420651129265 17.811073402210475 38-39 19.384760932244113 29.295636112445937 34.36080910619894 16.958793849111004 40-41 18.77963917912801 31.067716232734565 31.841525935314134 18.311118652823286 42-43 16.561192523600276 32.83427093689272 26.912200660177028 23.69233587932997 44-45 20.531689879106104 25.75983853783031 28.80234624715335 24.90612533591024 46-47 24.433046815871986 30.96806261337562 28.34473096190579 16.254159608846603 48-49 22.315326009669278 29.062947921195153 29.29082694934178 19.33089911979379 50-51 14.680424848820673 30.596563307609948 35.91212903438945 18.81088280917993 52-53 19.738035529259832 35.05969095850915 25.6210863003619 19.581187211869118 54-55 16.440062530127538 28.577424581668343 29.704138190981183 25.278374697222933 56-57 22.071458126354983 27.189952998548428 27.87353538175256 22.865053493344025 58-59 27.698986105895607 26.67562898986106 25.82868944799099 19.796695456252348 60-61 20.8752635974214 29.440903110299377 33.48273023845428 16.20110305382494 62-63 19.02649285113541 29.45640093716208 31.829343385798392 19.68776282590412 64-65 17.68582678170596 35.841463258944295 28.355598477924726 18.11711148142502 66-67 20.846576578808858 28.264746274058407 25.85975036998493 25.02892677714781 68-69 17.849079355687888 29.970364285773282 28.376327769347498 23.804228589191336 70-71 24.17224907685631 29.548631915318552 26.09864986012064 20.180469147704493 72-73 22.015043600613275 24.194773933035105 33.75544913812392 20.0347333282277 74-75 17.364482641073657 36.17112497015367 27.888937110214428 18.575455278558245 76-77 19.751662578267133 27.906987221217328 27.660031370676304 24.681318829839235 78-79 19.665108273998772 28.876695746984716 30.037605672088752 21.420590306927764 80-81 23.805851169685724 28.077564007729066 27.953652644155852 20.16293217842936 82-83 20.758538495794866 26.076403569575252 32.98229856554622 20.18275936908366 84-85 18.621594055077953 29.497184213570154 31.08308259890553 20.798139132446362 86-87 19.226350316290304 30.727617880464493 28.7174623485381 21.328569454707107 88-89 18.06957504120089 30.648427605871632 25.64385306794503 25.638144284982445 90-91 20.334328169848867 24.325067401238122 28.87680127883943 26.463803150073577 92-93 23.45490965708221 30.06698827648831 28.648435100084345 17.82966696634513 94-95 21.259382994416395 27.79950097566618 29.20711913342216 21.733996896495263 96-97 14.1475841117353 28.453227519746143 34.75629269817135 22.642895670347205 98-99 19.505261702856526 31.90143998172978 25.447064444591355 23.146233870822343 100-101 17.173896710793226 26.96991780739583 28.5945365208637 27.261648960947245 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1626.0 1 1148.0 2 956.5 3 1180.5 4 996.5 5 968.5 6 1471.5 7 2473.5 8 3276.5 9 3467.5 10 2853.5 11 2093.5 12 1934.5 13 1860.0 14 1709.5 15 1515.5 16 1374.0 17 1317.5 18 1297.0 19 1277.0 20 1312.5 21 1453.0 22 1596.0 23 1808.5 24 1980.0 25 2315.5 26 3182.0 27 4011.5 28 4199.5 29 4956.5 30 6377.5 31 7537.5 32 9879.5 33 13002.0 34 16205.0 35 18259.5 36 20543.5 37 22687.0 38 21438.0 39 19276.0 40 17036.5 41 14691.5 42 12444.0 43 11554.0 44 11459.5 45 11160.0 46 13939.0 47 48809.5 48 125440.5 49 117130.0 50 40945.0 51 11297.5 52 3709.5 53 1737.5 54 1119.0 55 937.0 56 844.5 57 785.5 58 713.0 59 618.0 60 518.5 61 419.5 62 375.0 63 351.0 64 306.5 65 299.5 66 253.0 67 184.0 68 136.5 69 110.5 70 100.5 71 70.5 72 59.5 73 80.5 74 78.5 75 48.0 76 37.5 77 30.5 78 23.0 79 21.0 80 14.0 81 10.0 82 8.0 83 5.5 84 5.0 85 3.5 86 1.5 87 1.5 88 1.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02342585042594503 2 0.010811930965820784 3 0.009460439595093184 4 0.0 5 0.0042046398200414154 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.009942471517319E-4 14-15 2.2524856178793298E-4 16-17 0.0 18-19 0.001952154202162086 20-21 0.0 22-23 7.5082853929311E-5 24-25 0.0 26-27 0.001651822786444842 28-29 0.0032285627189603725 30-31 0.00150165707858622 32-33 0.010811930965820784 34-35 0.0024777341796672625 36-37 0.001651822786444842 38-39 0.001651822786444842 40-41 7.5082853929311E-4 42-43 0.007358119685072478 44-45 0.0024777341796672625 46-47 4.5049712357586596E-4 48-49 0.001126242808939665 50-51 0.0 52-53 1.50165707858622E-4 54-55 0.0018019884943034639 56-57 0.015091653639791508 58-59 0.027179993122410578 60-61 0.021473696223782944 62-63 0.013665079415134602 64-65 0.019446459167691547 66-67 0.0038292255503948602 68-69 0.0017269056403741527 70-71 0.0 72-73 0.0 74-75 0.00300331415717244 76-77 0.0033787284268189948 78-79 0.011112262381538028 80-81 0.019896956291267413 82-83 0.020647784830560522 84-85 0.03303645572889684 86-87 0.013064416583700113 88-89 0.04354805527900038 90-91 0.049179269323698704 92-93 0.03266104145925028 94-95 0.0348384442232003 96-97 0.05375932341338668 98-99 0.05511081478411427 100-101 0.06321976300847985 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 665931.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.598856337968948 #Duplication Level Percentage of deduplicated Percentage of total 1 71.93321481023422 9.782094541326353 2 9.85214059342528 2.6795568910292507 3 4.262414558464648 1.7389188970028426 4 2.4889850815490453 1.3538940220533358 5 1.6475446946189778 1.12023618062532 6 1.2500138031559536 1.0199254877757606 7 0.9010700206495214 0.8577465232884489 8 0.7343278967303084 0.7988815658078691 9 0.6106516193862566 0.7473747280123616 >10 5.092812420631853 14.380168515957358 >50 0.6890535452025751 6.472442340122325 >100 0.4549520202299054 11.431064179321881 >500 0.02760631190715445 2.5971159174148672 >1k 0.03423182676487152 10.795863235079912 >5k 0.01214677723914796 11.369346073391988 >10k+ 0.008834019810289424 22.855370901790124 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 46691 7.011387065626919 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 20480 3.0753936969445785 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 20253 3.041306081260671 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14526 2.181307072354343 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 13156 1.975580052588031 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 13089 1.9655189501615031 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 12891 1.935786140005496 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 11115 1.6690918428485833 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9827 1.4756784111266783 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9525 1.4303283673533744 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 9353 1.4044998656016914 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7381 1.108373089704489 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6328 0.95024859932936 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6140 0.922017446251939 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5651 0.848586415109073 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5531 0.8305665301660383 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5351 0.8035367027514864 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5343 0.8023353770886174 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5282 0.7931752689092414 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4810 0.7222970547999719 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4561 0.6849057935431749 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4363 0.6551729833871678 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4246 0.637603595567709 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4209 0.6320474643769399 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3965 0.5954070316594362 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3740 0.5616197473912462 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3694 0.5547121248297496 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2878 0.4321769072171141 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2794 0.4195629877569898 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2271 0.34102632254693055 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2079 0.31219450663807513 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2000 0.300331415717244 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1958 0.29402445598718185 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1930 0.2898198161671404 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1855 0.2785573880777438 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1717 0.25783452039325394 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1651 0.2479235836745849 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1643 0.24672225801171593 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1612 0.24206712106809866 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1608 0.24146645823666413 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1486 0.2231462418779123 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1422 0.21353563657496047 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1366 0.20512635693487763 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1349 0.20257353990128105 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1334 0.20032105428340174 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1134 0.17028791271167734 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1098 0.16488194722876695 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1089 0.16353045585803935 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1029 0.15452051338652203 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1002 0.15046603927433924 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 998 0.14986537644290474 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 981 0.14731255940930815 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 895 0.13439830853346668 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 880 0.13214582291558735 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 816 0.12253521761263553 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 783 0.11757974925330102 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 775 0.11637842359043203 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 752 0.11292461230968374 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 729 0.10947080102893542 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 723 0.1085698067817837 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 722 0.10841964107392507 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 713 0.10706814970319747 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 686 0.10301367559101468 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 686 0.10301367559101468 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 672 0.10091135568099398 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.50165707858622E-4 0.0 12-13 0.0 0.0 0.0 1.50165707858622E-4 0.0 14-15 0.0 0.0 0.0 3.00331415717244E-4 0.0 16-17 0.0 0.0 0.0 3.00331415717244E-4 0.0 18-19 0.0 0.0 0.0 3.00331415717244E-4 0.0 20-21 0.0 0.0 0.0 3.00331415717244E-4 0.0 22-23 0.0 0.0 0.0 6.75745685363799E-4 0.0 24-25 0.0 0.0 0.0 0.001351491370727598 0.0 26-27 0.0 0.0 0.0 0.0020272370560913966 0.0 28-29 0.0 0.0 0.0 0.004580054089687971 0.0 30-31 0.0 0.0 0.0 0.008108948224365586 0.0 32-33 0.0 0.0 0.0 0.013439830853346667 0.0 34-35 0.0 0.0 0.0 0.01741922211160015 0.0 36-37 0.0 0.0 0.0 0.022299607617005366 0.0 38-39 0.0 0.0 0.0 0.02853148449313818 0.0 40-41 0.0 0.0 0.0 0.037841758380372736 0.0 42-43 0.0 0.0 0.0 0.05285832916623494 0.0 44-45 0.0 0.0 0.0 0.07868683091791792 0.0 46-47 0.0 0.0 0.0 0.13454847424132532 0.0 48-49 0.0 0.0 0.0 0.17434238682386013 0.0 50-51 0.0 0.0 0.0 0.2221701647768312 0.0 52-53 0.0 0.0 0.0 0.2760045710441472 0.0 54-55 0.0 0.0 0.0 0.3408010739851426 0.0 56-57 0.0 0.0 0.0 0.4215902248130812 0.0 58-59 0.0 0.0 0.0 0.5248291489658838 0.0 60-61 0.0 0.0 0.0 0.6488660236571057 0.0 62-63 0.0 0.0 0.0 0.7954277545271207 0.0 64-65 0.0 0.0 0.0 0.8923597189498612 0.0 66-67 0.0 0.0 0.0 1.0703060827623283 0.0 68-69 0.0 0.0 0.0 1.298407792999575 0.0 70-71 0.0 0.0 0.0 1.4390379784091745 0.0 72-73 0.0 0.0 0.0 1.6519729521527005 0.0 74-75 0.0 0.0 0.0 1.8526694207057488 0.0 76-77 0.0 0.0 0.0 1.9815866809023759 0.0 78-79 0.0 0.0 0.0 2.2080365683531777 0.0 80-81 0.0 0.0 0.0 2.4312578930850193 0.0 82-83 0.0 0.0 0.0 2.6661921430298334 0.0 84-85 0.0 0.0 0.0 3.0530190064736438 0.0 86-87 0.0 0.0 0.0 3.533023691643729 0.0 88-89 0.0 0.0 0.0 4.135038014448945 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 15 6.606406E-4 95.037186 1 CTTATGT 140 0.0 47.50075 2 CATGAGA 70 7.927338E-8 47.49005 4 GACATAT 180 0.0 44.878677 1 GGATGCC 65 2.5927984E-6 43.83368 8 ACAATCA 55 4.9693925E-5 43.16953 8 TACAATC 55 4.9693925E-5 43.16953 7 TATGTAC 155 0.0 42.894238 4 GATGATC 45 9.6356386E-4 42.21021 8 TACCTGG 125 3.6379788E-12 41.800663 2 GCTTATG 175 0.0 40.730225 1 GACAATG 230 0.0 39.227962 7 CATATAG 210 0.0 38.450104 3 GCAAGCG 25 0.0016122081 38.017735 94-95 GCTTAGT 25 0.0016169751 37.994896 46-47 GGACAAT 250 0.0 37.989185 6 CTTTAGG 140 1.0913936E-11 37.322018 2 ATGAGAG 90 5.7025136E-7 36.936707 5 GGATGAC 90 5.705879E-7 36.933933 8 ACCTGGG 130 2.055458E-10 36.536293 3 >>END_MODULE