##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936647_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 755222 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.62380598022833 31.0 31.0 34.0 30.0 34.0 2 31.723162196016535 33.0 31.0 34.0 30.0 34.0 3 31.711756278286384 33.0 31.0 34.0 30.0 34.0 4 35.34870938611428 37.0 35.0 37.0 33.0 37.0 5 35.38186519990148 37.0 35.0 37.0 33.0 37.0 6 35.39489315724383 37.0 35.0 37.0 33.0 37.0 7 35.33116355190924 37.0 35.0 37.0 33.0 37.0 8 35.3116183056108 37.0 35.0 37.0 33.0 37.0 9 36.82946074134493 39.0 37.0 39.0 33.0 39.0 10-11 36.71977908482539 39.0 37.0 39.0 32.0 39.0 12-13 36.68270521780351 39.0 37.0 39.0 32.0 39.0 14-15 37.7720054500531 40.0 37.0 41.0 32.5 41.0 16-17 37.677867567417266 40.0 37.0 41.0 32.0 41.0 18-19 37.758153231764965 40.0 37.0 41.0 32.0 41.0 20-21 37.686780840600505 40.0 37.0 41.0 32.0 41.0 22-23 37.789788962715605 40.0 37.0 41.0 32.0 41.0 24-25 37.84422858444272 40.0 37.0 41.0 33.0 41.0 26-27 37.758666325928004 40.0 37.0 41.0 32.5 41.0 28-29 37.651881830772936 40.0 37.0 41.0 32.0 41.0 30-31 37.441061701062736 39.5 37.0 41.0 32.0 41.0 32-33 37.24328674217647 39.0 36.0 41.0 32.0 41.0 34-35 37.07133465391633 39.0 36.0 41.0 31.0 41.0 36-37 36.87304924909497 39.0 35.0 41.0 31.0 41.0 38-39 36.68947673664167 39.0 35.0 41.0 30.0 41.0 40-41 36.53471429592888 39.0 35.0 41.0 30.0 41.0 42-43 36.295711724499554 39.0 35.0 40.0 30.0 41.0 44-45 35.96495930997773 39.0 35.0 40.0 29.5 41.0 46-47 35.62382716605184 39.0 35.0 40.0 27.5 41.0 48-49 35.380808556954115 39.0 35.0 41.0 27.0 41.0 50-51 34.42717836609633 38.0 34.0 39.5 25.0 40.5 52-53 34.55359351290084 38.0 34.5 39.5 24.5 40.5 54-55 35.06386267878848 39.0 35.0 40.0 25.5 41.0 56-57 35.009239667276645 39.0 35.0 41.0 24.5 41.0 58-59 34.83789865761325 39.0 35.0 40.5 24.0 41.0 60-61 34.58548214961958 38.5 34.5 40.0 23.0 41.0 62-63 34.27827181941204 38.0 34.0 40.0 22.0 41.0 64-65 33.963993368837244 38.0 34.0 40.0 21.5 41.0 66-67 33.51458710154101 37.5 33.0 40.0 19.5 41.0 68-69 33.13728148809224 37.0 33.0 39.0 18.5 41.0 70-71 32.7507679861021 36.5 32.5 39.0 16.0 41.0 72-73 32.34168297533705 36.0 32.0 39.0 13.0 40.0 74-75 31.877517471683824 35.5 32.0 38.0 8.0 40.0 76-77 31.416342479429886 35.0 31.0 37.0 7.0 39.0 78-79 30.900625908673213 35.0 31.0 37.0 2.0 39.0 80-81 30.402437296582992 35.0 30.5 36.0 2.0 39.0 82-83 29.963435916856234 34.5 30.0 36.0 2.0 37.5 84-85 29.560035327360694 34.0 30.0 35.5 2.0 37.0 86-87 29.231792770867372 34.0 29.0 35.0 2.0 36.5 88-89 28.808196795114547 34.0 29.0 35.0 2.0 36.0 90-91 28.405223232374055 34.0 29.0 35.0 2.0 36.0 92-93 28.00458937901703 34.0 29.0 35.0 2.0 35.5 94-95 27.56996684418621 34.0 27.0 35.0 2.0 35.0 96-97 27.052292570926163 34.0 26.0 35.0 2.0 35.0 98-99 26.416363665253396 34.0 25.0 35.0 2.0 35.0 100-101 23.57057328838408 30.0 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4989320196680751 1101 2 0.47364512259634495 1101 3 0.5737598314382026 1101 4 0.5700483763161799 1101 5 0.502015970934437 1101 6 0.6235528753764825 1101 7 0.6000671142069152 1101 8 0.46904938765829485 1101 9 0.6590225667991874 1101 10-11 0.7523594699112834 1101 12-13 0.6986325264478097 1101 14-15 0.984467456672121 1101 16-17 0.9173172006761519 1101 18-19 0.7831867950956948 1101 20-21 0.9436987909062609 1101 22-23 0.8313020121269474 1101 24-25 0.7817784144597297 1101 26-27 0.8645162531400246 1101 28-29 0.9794369860290857 1101 30-31 0.8439895529185577 1101 32-33 0.7810241905079138 1101 34-35 0.6373296813273797 1101 36-37 0.5627229893639267 1101 38-39 0.5206296265887005 1101 40-41 0.47445655176863966 1101 42-43 0.7454350780071621 1101 44-45 0.7075700561102067 1101 46-47 0.8902680784705979 1101 48-49 1.0295053611086544 1101 50-51 0.8578524385707098 1101 52-53 0.9923063048156848 1101 54-55 0.7988408425716429 1101 56-57 0.5593276585886784 1101 58-59 0.8332129179294796 1101 60-61 0.8544477066775755 1101 62-63 0.8821805295173419 1101 64-65 0.807592892564962 1101 66-67 1.0203577498425247 1101 68-69 0.9028803244992574 1101 70-71 0.8620424987869129 1101 72-73 0.7220533488665097 1101 74-75 0.8918829691904904 1101 76-77 0.977335242625383 1101 78-79 0.5754431168923198 1101 80-81 0.9336845534309859 1101 82-83 0.9315569150365732 1101 84-85 1.016088531709027 1101 86-87 0.980474181671326 1101 88-89 1.1039864556744803 1101 90-91 1.1918266280433585 1101 92-93 0.8670114928454851 1101 94-95 0.7932188977237864 1101 96-97 0.8111876782514535 1101 98-99 0.8700460932833955 1101 100-101 0.9844189904868692 1102 1 0.17697744608506838 1102 2 0.16361632044497298 1102 3 0.27325789474182116 1102 4 0.2559414340045194 1102 5 0.2919512615916915 1102 6 0.35755545763832686 1102 7 0.12968290725925868 1102 8 0.29759942471146417 1102 9 0.38042554480411894 1102 10-11 0.5057122084076155 1102 12-13 0.5027496297306371 1102 14-15 0.44948010058737964 1102 16-17 0.21687561862057692 1102 18-19 0.053580933819688426 1102 20-21 0.1249537090455064 1102 22-23 0.08456383667066092 1102 24-25 0.11082274838133088 1102 26-27 0.020219766696897068 1102 28-29 -0.03627584811789575 1102 30-31 0.27031181614118793 1102 32-33 0.294214353465442 1102 34-35 0.2027311924853521 1102 36-37 0.06090012641480058 1102 38-39 0.06186254202344088 1102 40-41 -0.0017820553379337412 1102 42-43 -0.056737689062131835 1102 44-45 0.23593444534415653 1102 46-47 0.42626847574165083 1102 48-49 0.5546144196526583 1102 50-51 0.5531615842087518 1102 52-53 0.8232667075874076 1102 54-55 0.7933562100855625 1102 56-57 0.7680806094722215 1102 58-59 0.9895704007589714 1102 60-61 0.8235239750800147 1102 62-63 1.0464780855363855 1102 64-65 0.7122126965269757 1102 66-67 0.6276545495185957 1102 68-69 0.7815873600168146 1102 70-71 0.8185138150528672 1102 72-73 0.5078738281893109 1102 74-75 0.76355295918232 1102 76-77 0.6898691443619178 1102 78-79 0.7117773746448322 1102 80-81 0.6152947235699529 1102 82-83 0.414313823350998 1102 84-85 -0.12667464126431582 1102 86-87 0.24100453852769022 1102 88-89 0.5099508693558832 1102 90-91 0.5171259183033428 1102 92-93 0.7997733670480791 1102 94-95 0.6291841517862657 1102 96-97 0.4693359367526746 1102 98-99 0.3658541196866416 1102 100-101 0.6960086094886506 1103 1 0.34710352377117104 1103 2 0.3139500554964201 1103 3 0.3428617388751931 1103 4 0.33004452105374327 1103 5 0.410921627155183 1103 6 0.4840703984621584 1103 7 0.4598411223947636 1103 8 0.6142367350541633 1103 9 0.31473845231624864 1103 10-11 0.4872849348375752 1103 12-13 0.2616500036375058 1103 14-15 0.6157666801121167 1103 16-17 0.5699603502419777 1103 18-19 0.6733462377349042 1103 20-21 0.5581564839906505 1103 22-23 0.4146169870677525 1103 24-25 0.3172147016584219 1103 26-27 0.3924347785939375 1103 28-29 0.45139913102366336 1103 30-31 0.551033209176012 1103 32-33 0.20718444091638588 1103 34-35 0.38901406384285053 1103 36-37 0.5409246930227525 1103 38-39 0.41043567017152327 1103 40-41 0.31916290927996727 1103 42-43 0.1114578240555133 1103 44-45 0.1858038869559735 1103 46-47 0.43936989411562166 1103 48-49 0.3505431610388925 1103 50-51 0.5829791114792329 1103 52-53 0.7261073181989488 1103 54-55 0.501540444194525 1103 56-57 0.19566396598160196 1103 58-59 0.2287834967696014 1103 60-61 0.3514093927123483 1103 62-63 0.615908109330455 1103 64-65 0.46243904923548484 1103 66-67 0.3312043471144648 1103 68-69 0.3741540297736208 1103 70-71 0.3982108268880964 1103 72-73 0.47722582596441043 1103 74-75 0.5305255496461427 1103 76-77 0.5612606341181028 1103 78-79 0.5073059429832654 1103 80-81 0.4872084468788742 1103 82-83 0.16913057808663368 1103 84-85 0.589655200211535 1103 86-87 0.5444577692683978 1103 88-89 0.3244019060976413 1103 90-91 0.31741639140367894 1103 92-93 0.3480220305628059 1103 94-95 0.33777242161723464 1103 96-97 0.5595216541285275 1103 98-99 0.48682612302399164 1103 100-101 0.6571439266914574 1104 1 0.3855607352014587 1104 2 0.3392631883247432 1104 3 0.31302349291904363 1104 4 0.39062728562286253 1104 5 0.314400464126102 1104 6 0.29657821115748106 1104 7 0.4827291945965655 1104 8 0.2815034167212005 1104 9 0.39510921158522905 1104 10-11 0.4701095054518305 1104 12-13 0.4837836819794674 1104 14-15 0.2632359918449083 1104 16-17 0.2802187317015168 1104 18-19 0.1680554804659664 1104 20-21 0.023389989828295654 1104 22-23 0.17786106816977565 1104 24-25 0.28978276586002494 1104 26-27 0.10671201052473123 1104 28-29 0.21160876126442218 1104 30-31 0.31528669051304803 1104 32-33 0.23297258561999712 1104 34-35 0.2164785004537677 1104 36-37 0.12303299573031978 1104 38-39 0.15768560141980714 1104 40-41 0.11612970844994663 1104 42-43 0.4785170005349215 1104 44-45 0.3191697211018223 1104 46-47 0.24385875633717546 1104 48-49 0.3885847370782969 1104 50-51 0.169693779345927 1104 52-53 0.2999685397294911 1104 54-55 0.21788022768910764 1104 56-57 0.356639865379087 1104 58-59 0.2834723639912795 1104 60-61 0.39359794742621546 1104 62-63 0.19081935711164988 1104 64-65 0.29530049577164874 1104 66-67 0.2041449206288064 1104 68-69 0.21979392077088988 1104 70-71 0.271638912590241 1104 72-73 0.3950987727880815 1104 74-75 0.46809273882587377 1104 76-77 0.23369932008525396 1104 78-79 0.3662305410982185 1104 80-81 0.38686218822240903 1104 82-83 0.05923095559607461 1104 84-85 -0.013781760712490154 1104 86-87 0.17603918380375916 1104 88-89 0.1649354194354764 1104 90-91 0.05217851045665256 1104 92-93 -0.014765164117061857 1104 94-95 -0.06133755613220515 1104 96-97 -0.028046160066828918 1104 98-99 0.07986594901963784 1104 100-101 0.1649472467744566 1105 1 0.2591345596587331 1105 2 0.15881691227950512 1105 3 0.28199053657403894 1105 4 0.26514877645169577 1105 5 0.06905774557054656 1105 6 0.15319067795797991 1105 7 0.4368203700769442 1105 8 0.5400604699775613 1105 9 0.35661961721137914 1105 10-11 0.3826545507777226 1105 12-13 0.2668241947168468 1105 14-15 0.3475019244857833 1105 16-17 0.4620615687153702 1105 18-19 0.3801425909563818 1105 20-21 0.29890528341783096 1105 22-23 0.11575151294950814 1105 24-25 0.2802531076433823 1105 26-27 -0.040277590083775294 1105 28-29 -0.048174958824859004 1105 30-31 -0.08291399031043056 1105 32-33 -0.1406838479124204 1105 34-35 0.040483762495988174 1105 36-37 0.054151055009349136 1105 38-39 0.09752904942907747 1105 40-41 -0.03967256594934554 1105 42-43 -0.10851088080726612 1105 44-45 -0.26802961303785366 1105 46-47 -0.09300390685471172 1105 48-49 -0.08301176902542551 1105 50-51 0.1766438522912992 1105 52-53 0.14785250047614795 1105 54-55 0.2141004728354332 1105 56-57 0.1486302141452498 1105 58-59 0.19325857793943868 1105 60-61 0.010968297938077853 1105 62-63 -0.06806501512172503 1105 64-65 0.10218541665397396 1105 66-67 -0.03754897066388452 1105 68-69 -0.007399739839847541 1105 70-71 -0.05937253422198552 1105 72-73 -0.2307849884498694 1105 74-75 -8.961260942577098E-4 1105 76-77 0.11592527616839732 1105 78-79 0.20730470846108773 1105 80-81 0.29331130726676236 1105 82-83 0.01186634095961736 1105 84-85 -0.19854708859258707 1105 86-87 -0.03812250553623997 1105 88-89 -0.1926777082376283 1105 90-91 -0.29978914419377034 1105 92-93 -0.44872778117256473 1105 94-95 -0.444891492064972 1105 96-97 -0.23352922053043912 1105 98-99 0.2743386225610429 1105 100-101 0.3681515436932976 1106 1 0.2908855713874985 1106 2 0.33743893294824545 1106 3 0.47779530857458496 1106 4 0.3475575307180705 1106 5 0.33964963462739917 1106 6 0.3892237504760345 1106 7 0.42449581826407723 1106 8 0.4887625732959009 1106 9 0.6257980966234484 1106 10-11 0.7114061868152248 1106 12-13 0.5109613968629461 1106 14-15 0.5666921294935605 1106 16-17 0.5168280233705929 1106 18-19 0.48066739059130725 1106 20-21 0.14218318954335274 1106 22-23 0.27100077693069125 1106 24-25 0.17616230082732187 1106 26-27 0.32576144030453236 1106 28-29 0.373679491672533 1106 30-31 0.5007077292957192 1106 32-33 0.5153061885003041 1106 34-35 0.41342267214234596 1106 36-37 0.14988420580368 1106 38-39 -0.10025560525212285 1106 40-41 -0.07282351873321602 1106 42-43 0.12083860215734177 1106 44-45 0.1456288045137768 1106 46-47 0.30652120651912185 1106 48-49 0.30606726465872924 1106 50-51 0.2982604633406183 1106 52-53 0.19356647833695462 1106 54-55 0.3097837106210122 1106 56-57 0.361432346302216 1106 58-59 0.4540675172800732 1106 60-61 0.4036481986824967 1106 62-63 0.6366163497376149 1106 64-65 0.6949760767416322 1106 66-67 0.6990323526805113 1106 68-69 0.631292666093934 1106 70-71 0.5269183429476456 1106 72-73 0.34171563744341427 1106 74-75 0.3857894599795344 1106 76-77 0.2909836664659444 1106 78-79 0.227498085717869 1106 80-81 0.4708629711921759 1106 82-83 0.08570431857957317 1106 84-85 0.33110599222846204 1106 86-87 0.38465810856806826 1106 88-89 0.30498271851067216 1106 90-91 -0.08946681707500304 1106 92-93 0.06531916557738526 1106 94-95 0.36610537371498353 1106 96-97 0.2818582470261042 1106 98-99 0.27933150797662165 1106 100-101 -0.05295500394273134 1107 1 0.07540768277285892 1107 2 0.07801252586377316 1107 3 0.1605600958830351 1107 4 0.39624623168736406 1107 5 0.19759183393576052 1107 6 0.2227704812755391 1107 7 0.27841186461757417 1107 8 0.3603378685227767 1107 9 0.30847687582766525 1107 10-11 0.27676747765082155 1107 12-13 0.18738268078543996 1107 14-15 0.14833572813755325 1107 16-17 -0.03554847791709648 1107 18-19 0.013864896031719809 1107 20-21 0.26067233548287305 1107 22-23 0.2555030902241384 1107 24-25 0.2714381839926219 1107 26-27 0.374979172254605 1107 28-29 0.35652249767200317 1107 30-31 0.3911504438413118 1107 32-33 0.17731868077174084 1107 34-35 0.19813870746084916 1107 36-37 0.39433790017631765 1107 38-39 0.328127231424979 1107 40-41 0.325683672680249 1107 42-43 0.4313505775687645 1107 44-45 0.42527441866844384 1107 46-47 0.46451971679384485 1107 48-49 0.5694565357157018 1107 50-51 0.5868582229106423 1107 52-53 0.736112091031103 1107 54-55 0.7245407539544857 1107 56-57 0.6829298285366789 1107 58-59 0.7740582348959961 1107 60-61 0.6558718327358619 1107 62-63 0.6389702130431516 1107 64-65 0.8605936068160673 1107 66-67 0.6750149952713898 1107 68-69 0.8046176582639575 1107 70-71 0.6360171889785349 1107 72-73 0.634890488673733 1107 74-75 0.5743881932262127 1107 76-77 0.5650630957616052 1107 78-79 0.3799977571020001 1107 80-81 0.5655303064501425 1107 82-83 0.5683266245379848 1107 84-85 0.19953306628143608 1107 86-87 0.24364133049656544 1107 88-89 0.1519272400378533 1107 90-91 0.3745639446160176 1107 92-93 0.3299571769401908 1107 94-95 0.43501571594610056 1107 96-97 0.4975032834659565 1107 98-99 0.4080483157452619 1107 100-101 0.37015131667925516 1108 1 0.042062599360964015 1108 2 0.18561591972367353 1108 3 0.19563817925394034 1108 4 0.1080271883623638 1108 5 0.19848702596757306 1108 6 0.2898656159232402 1108 7 0.34054245444418996 1108 8 0.20636319950609305 1108 9 0.27155758247192097 1108 10-11 0.28059499742784766 1108 12-13 0.12878449248359658 1108 14-15 0.30861854979595904 1108 16-17 0.26191268534067547 1108 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0.8855587445830615 2201 94-95 0.8181911113013456 2201 96-97 1.0292962750000747 2201 98-99 1.0808694605532096 2201 100-101 1.3016473133725093 2202 1 0.5100329173278269 2202 2 0.5563183688758926 2202 3 0.5000866394023546 2202 4 0.511386025687905 2202 5 0.4395389695255716 2202 6 0.5811401747298106 2202 7 0.4905828250851201 2202 8 0.5853258662752907 2202 9 0.7053628037414796 2202 10-11 0.7717765115191213 2202 12-13 0.6603174233921294 2202 14-15 0.9886816048922711 2202 16-17 0.9630922770204791 2202 18-19 0.7175805192474769 2202 20-21 0.8579321883057887 2202 22-23 0.7742743023981262 2202 24-25 0.5089986306428145 2202 26-27 0.8255003802637759 2202 28-29 0.7574270960851237 2202 30-31 0.8647099091090027 2202 32-33 0.5618540309874831 2202 34-35 0.6255815245132723 2202 36-37 0.6622689550262066 2202 38-39 0.6249197159735047 2202 40-41 0.5764640080768118 2202 42-43 0.630637106427919 2202 44-45 0.9002597660823923 2202 46-47 0.8051238122484321 2202 48-49 0.8513866788491313 2202 50-51 0.7461390270385024 2202 52-53 0.6533609487351839 2202 54-55 0.8125168494151396 2202 56-57 0.921932059364508 2202 58-59 0.7045534847320525 2202 60-61 0.6825516551783224 2202 62-63 0.7485768573662028 2202 64-65 0.5784968709730407 2202 66-67 0.8049641239158021 2202 68-69 0.906256627630583 2202 70-71 0.7027837607883285 2202 72-73 0.7656733381057279 2202 74-75 0.6163983311498313 2202 76-77 0.6066060168587448 2202 78-79 0.53946635664796 2202 80-81 0.4858149376668912 2202 82-83 0.5005226344911442 2202 84-85 0.5090910958228712 2202 86-87 0.7413650682917563 2202 88-89 0.7193268641441186 2202 90-91 0.8458024705431413 2202 92-93 1.031685803474172 2202 94-95 0.8276717035152537 2202 96-97 1.054178880511511 2202 98-99 1.22832052823275 2202 100-101 1.4062648299226197 2203 1 0.5577087218570256 2203 2 0.40137200415598784 2203 3 0.5429948634786328 2203 4 0.48874001853653226 2203 5 0.6588476703598971 2203 6 0.6359673499383902 2203 7 0.6812860432019292 2203 8 0.6759098948807747 2203 9 0.8245523150644871 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0.8861054209111892 2203 78-79 1.0561528882331785 2203 80-81 0.8481510520888236 2203 82-83 0.9713212042170092 2203 84-85 1.0698777465976015 2203 86-87 0.9630575593525421 2203 88-89 1.2640229308902455 2203 90-91 1.3362673096373037 2203 92-93 1.184844325524228 2203 94-95 1.3062175914771146 2203 96-97 1.2150847207975666 2203 98-99 1.248582745157659 2203 100-101 1.3287916475626673 2204 1 0.6333560844969526 2204 2 0.6212737312353767 2204 3 0.4772083879825928 2204 4 0.4822065052504101 2204 5 0.4118361720776278 2204 6 0.4961606823369067 2204 7 0.611399506942611 2204 8 0.47365288957566776 2204 9 0.6597596697584507 2204 10-11 0.7795734920515045 2204 12-13 0.8787117537106681 2204 14-15 0.7489427117187972 2204 16-17 0.7681239440377112 2204 18-19 0.7628893310916425 2204 20-21 0.5952412805958645 2204 22-23 0.6256299370238523 2204 24-25 0.6361530979124481 2204 26-27 0.6122913779500934 2204 28-29 0.5899526867743248 2204 30-31 0.6560961298666754 2204 32-33 0.5910189448672227 2204 34-35 0.6224790916450118 2204 36-37 0.6401436716589828 2204 38-39 0.6417880992028415 2204 40-41 0.633711489906247 2204 42-43 0.6227707447461768 2204 44-45 0.44225142865922606 2204 46-47 0.5177281030708443 2204 48-49 0.6444504627655547 2204 50-51 0.6693304430566442 2204 52-53 0.5117196974790232 2204 54-55 0.7251192413850376 2204 56-57 0.6779492308338106 2204 58-59 0.7945941493888498 2204 60-61 1.0010746692707286 2204 62-63 0.6671409158859163 2204 64-65 0.847014334573494 2204 66-67 0.7377104196927533 2204 68-69 0.8126417382898623 2204 70-71 0.8266751230543292 2204 72-73 0.9589166366170332 2204 74-75 0.5458990497924674 2204 76-77 0.7099839294063734 2204 78-79 0.6092594109376108 2204 80-81 0.5837935361384581 2204 82-83 0.7591675257015034 2204 84-85 0.6340837925439864 2204 86-87 0.5728178073134629 2204 88-89 0.615336936826349 2204 90-91 0.6632424083776307 2204 92-93 0.650617509052708 2204 94-95 0.6080387370986529 2204 96-97 0.7375926959781012 2204 98-99 0.9836526521431033 2204 100-101 0.7992184803935345 2205 1 0.41346812931488586 2205 2 0.3643409581261281 2205 3 0.409386259131054 2205 4 0.4151546845221219 2205 5 0.47549163426250374 2205 6 0.4633525590876033 2205 7 0.41938270021991997 2205 8 0.4676304806120868 2205 9 0.657133619338282 2205 10-11 0.6521925396705512 2205 12-13 0.5807775800412003 2205 14-15 0.7342368293658552 2205 16-17 0.7349656807323868 2205 18-19 0.7670384907555103 2205 20-21 0.7805178912401232 2205 22-23 0.7009975840826783 2205 24-25 0.6984780278844269 2205 26-27 0.6676835348128378 2205 28-29 0.5295885411160555 2205 30-31 0.502454673284042 2205 32-33 0.599597376239764 2205 34-35 0.509874049628209 2205 36-37 0.5217438117150053 2205 38-39 0.5549008443008816 2205 40-41 0.39775210615275114 2205 42-43 0.5283554786397389 2205 44-45 0.6087605323006784 2205 46-47 0.9772519125946602 2205 48-49 0.81362063083278 2205 50-51 0.7096315635048214 2205 52-53 0.7048919663865831 2205 54-55 0.6554938912449799 2205 56-57 0.6690381664080363 2205 58-59 0.7717650177361932 2205 60-61 0.9726958177301199 2205 62-63 0.702715145577784 2205 64-65 0.7683203569824641 2205 66-67 1.070045853866425 2205 68-69 0.8811991612590546 2205 70-71 1.008782966191582 2205 72-73 0.8024390455806127 2205 74-75 0.6455664167392499 2205 76-77 0.5262304280058068 2205 78-79 0.6133560495930759 2205 80-81 0.5776135641496651 2205 82-83 0.7737858730404383 2205 84-85 0.7965137645327864 2205 86-87 0.7437736896664084 2205 88-89 0.5841744718403561 2205 90-91 0.5413236408706297 2205 92-93 0.597728853590521 2205 94-95 0.5581437791154578 2205 96-97 0.7659715475187205 2205 98-99 0.6394824840758737 2205 100-101 0.3944040546232266 2206 1 0.3640054238787265 2206 2 0.2915006715690289 2206 3 0.02099603578507825 2206 4 0.3357193705735284 2206 5 0.3755548453876614 2206 6 0.34118652453219056 2206 7 0.5573199105022653 2206 8 0.5530603162631564 2206 9 0.646703783687208 2206 10-11 0.4817964166195594 2206 12-13 0.6091751780184396 2206 14-15 0.6659424762263626 2206 16-17 0.5131104342089969 2206 18-19 0.5726432105195158 2206 20-21 0.592248822550701 2206 22-23 0.5941444445967861 2206 24-25 0.7058263211840554 2206 26-27 0.6303995061571328 2206 28-29 0.7150131091733698 2206 30-31 0.6708175051424519 2206 32-33 0.46411486465105867 2206 34-35 0.6854113441920546 2206 36-37 0.5642585609775423 2206 38-39 0.6415580292987713 2206 40-41 0.5608684146230445 2206 42-43 0.4416298149229192 2206 44-45 0.42965180916540646 2206 46-47 0.43381533442356357 2206 48-49 0.7903589367746306 2206 50-51 0.6506661189199079 2206 52-53 0.4394157759103905 2206 54-55 0.48845322542113223 2206 56-57 0.5681795486246486 2206 58-59 0.4934675040404528 2206 60-61 0.5894889066470981 2206 62-63 0.6248653773519166 2206 64-65 0.537178342654606 2206 66-67 0.6689396591761536 2206 68-69 0.6405386050011472 2206 70-71 0.6548849468068383 2206 72-73 0.6665667320534396 2206 74-75 0.5676534373882163 2206 76-77 0.6657005080732326 2206 78-79 0.5644569766895771 2206 80-81 0.8077863412250963 2206 82-83 0.7843737365044063 2206 84-85 0.7481783241619446 2206 86-87 0.7842024717987286 2206 88-89 0.8727332581867522 2206 90-91 0.8516007163025741 2206 92-93 0.7790236281375087 2206 94-95 0.80917095989928 2206 96-97 1.0270229592338467 2206 98-99 0.9122016159764215 2206 100-101 0.7175498616185898 2207 1 0.657073192157867 2207 2 0.6447357182606694 2207 3 0.4976408174599598 2207 4 0.47879426674906256 2207 5 0.5307314997254124 2207 6 0.6903484875705246 2207 7 0.6886737251800881 2207 8 0.7645724172380355 2207 9 0.788882601459008 2207 10-11 0.9296664288544747 2207 12-13 0.8865020445481946 2207 14-15 0.8343762345703141 2207 16-17 0.9495147159598574 2207 18-19 0.836626028372784 2207 20-21 0.9711865936653297 2207 22-23 0.8054740400925837 2207 24-25 0.86624002115758 2207 26-27 0.821308070128822 2207 28-29 0.7156679356991731 2207 30-31 0.6799772436504767 2207 32-33 0.8145911804529007 2207 34-35 0.7974736247742484 2207 36-37 0.8218920680435602 2207 38-39 0.844383496097656 2207 40-41 1.0160872097109035 2207 42-43 0.8448008672040856 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72-73 -1.5533181753405394 2316 74-75 -1.590060046960982 2316 76-77 -1.9669487289028567 2316 78-79 -2.8904593290173857 2316 80-81 -2.533803297677032 2316 82-83 -2.511635672627012 2316 84-85 -2.4711677342962552 2316 86-87 -2.3426334141587297 2316 88-89 -2.5989832174648733 2316 90-91 -2.553026476225387 2316 92-93 -3.207999093325949 2316 94-95 -3.1864405300180287 2316 96-97 -3.4532321995538844 2316 98-99 -4.480336017095087 2316 100-101 -5.594354727578178 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1211.0 3 398.0 4 361.0 5 360.0 6 429.0 7 556.0 8 643.0 9 875.0 10 1185.0 11 1738.0 12 2491.0 13 3390.0 14 4055.0 15 5904.0 16 7845.0 17 11115.0 18 8012.0 19 4857.0 20 4540.0 21 4512.0 22 4959.0 23 5420.0 24 6270.0 25 7071.0 26 8402.0 27 9771.0 28 11645.0 29 14136.0 30 17471.0 31 21572.0 32 27280.0 33 35458.0 34 46860.0 35 65327.0 36 93444.0 37 144360.0 38 147095.0 39 24202.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.370440996141184 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32.72909885884231 19.430069994888758 44-45 18.649303737169966 29.570762059055056 32.32694725510305 19.452986948671928 46-47 19.497387495464864 30.020166469371595 31.69240771489935 18.79003832026419 48-49 18.83953960282965 30.091000274755288 31.69524011294759 19.37422000946747 50-51 18.264224347973474 30.26533930803619 32.20208584927647 19.26835049471387 52-53 18.672462079330504 31.26536854719642 31.169102020008076 18.893067353465 54-55 18.642766058028318 30.308216683903105 31.28274868475588 19.766268573312693 56-57 19.136704841428422 30.824034607546764 30.567682409286938 19.471578141737872 58-59 19.244319351778103 29.869559776660594 31.417217367929357 19.468903503631946 60-61 19.220761680034006 30.376827829486004 31.73342390548716 18.66898658499283 62-63 18.768144583322574 30.202848579583257 31.9540636252952 19.07494321179897 64-65 18.762521301937333 30.728681842076345 31.2657488682001 19.243047987786227 66-67 18.682434177330858 30.48760622807401 30.367158126704254 20.46280146789088 68-69 18.37903497680863 30.153547189781094 31.399516104141593 20.067901729268677 70-71 19.276531452375163 30.188040619264278 31.15298328770698 19.382444640653578 72-73 18.907569146012808 29.337575568960606 31.85424543606518 19.900609848961402 74-75 18.366167036317627 30.936109876733713 30.758028401644253 19.939694685304403 76-77 18.451142418555243 30.221013720148182 31.36906125789879 19.958782603397786 78-79 18.631385737176196 29.844542493639352 31.314066026218924 20.210005742965528 80-81 18.780683981984154 30.251805758551097 30.684644526275758 20.282865733188988 82-83 18.84250532197449 30.349296386621077 31.10086383561027 19.70733445579416 84-85 18.59097031203027 29.989391950861293 30.783009110208493 20.63662862689995 86-87 18.150750015395626 30.268466641812935 31.362715033880313 20.21806830891112 88-89 18.95882569905724 29.71722508030707 30.786462985906617 20.537486234729073 90-91 18.759963467561565 29.13051339467551 31.550224486403323 20.5592986513596 92-93 19.36001684688998 29.61615712108901 31.504147818215174 19.51967821380584 94-95 18.872775742390363 28.91513372528428 31.642942797189555 20.569147735135804 96-97 18.78927938815038 28.905855281672654 31.37351631433443 20.931349015842535 98-99 18.53887557510893 29.374504045137023 30.812988420323318 21.273631959430727 100-101 18.439081073525116 29.17711468428713 31.22977335558416 21.154030886603593 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 418.0 1 295.0 2 213.5 3 276.5 4 266.5 5 279.5 6 419.0 7 761.0 8 1216.0 9 1441.0 10 1310.5 11 1076.0 12 1057.0 13 1109.0 14 1147.5 15 1205.0 16 1293.5 17 1412.0 18 1549.0 19 1695.0 20 1889.0 21 2156.5 22 2475.5 23 2906.5 24 3227.0 25 4159.5 26 6372.0 27 8940.0 28 10028.5 29 11234.0 30 14131.5 31 17066.5 32 21078.0 33 26948.0 34 33059.5 35 37326.5 36 43131.5 37 48415.5 38 46555.0 39 42515.5 40 38077.5 41 33455.0 42 29924.0 43 27357.5 44 25823.0 45 25235.5 46 23605.5 47 23583.5 48 32350.5 49 30042.0 50 16674.5 51 10172.5 52 7191.0 53 5746.5 54 4796.0 55 4049.0 56 3332.0 57 2753.5 58 2243.5 59 1858.5 60 1461.0 61 1147.5 62 824.0 63 500.0 64 313.0 65 212.0 66 152.5 67 117.5 68 81.0 69 47.5 70 42.0 71 40.5 72 27.5 73 25.5 74 23.5 75 20.5 76 18.5 77 10.0 78 9.0 79 11.5 80 8.0 81 4.5 82 3.0 83 0.5 84 0.0 85 0.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00900397499013535 2 0.00900397499013535 3 0.011652202928410455 4 0.014565253660513068 5 0.027541570558061074 6 0.04528469774450426 7 0.006223335654946493 8 0.02462851982595846 9 0.03575107716671389 10-11 0.037141396834308324 12-13 0.02707813066886293 14-15 0.027806393351888583 16-17 0.027342953462690445 18-19 0.02111961780774395 20-21 0.050713565017968226 22-23 0.010592911753100414 24-25 0.0029130507321026137 26-27 0.0015227310645081844 28-29 0.0 30-31 0.002118582350620083 32-33 0.0015227310645081844 34-35 6.620569845687759E-5 36-37 2.6482279382751035E-4 38-39 0.0 40-41 0.0015889367629650618 42-43 0.0031778735259301236 44-45 0.0015227310645081844 46-47 0.0010592911753100414 48-49 5.958512861118983E-4 50-51 0.005031633082722696 52-53 0.0035751077166713894 54-55 0.005164044479636452 56-57 0.0014565253660513069 58-59 0.006421952750317124 60-61 0.003045462129016369 62-63 4.63439889198143E-4 64-65 0.0011917025722237966 66-67 0.016551424614219396 68-69 0.040716504550979714 70-71 0.04746948579358123 72-73 0.015492133438909353 74-75 0.03052082698862056 76-77 0.02661469077966479 78-79 0.05104459351025261 80-81 0.0732235024933066 82-83 0.04409299517228047 84-85 0.017875538583356946 86-87 0.018206567075641337 88-89 0.020060326632433907 90-91 0.0366117512466533 92-93 0.024694725524415335 94-95 0.014631459358969945 96-97 0.01780933288490007 98-99 0.048330159873520635 100-101 0.06534502437693818 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 755222.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.59192595496086 #Duplication Level Percentage of deduplicated Percentage of total 1 75.87254921178086 23.96959956712705 2 11.782737286628068 7.444787278118207 3 4.158676237460852 3.9414177529355534 4 2.0030363389544292 2.531191028213768 5 1.1243805664906128 1.7760673800884192 6 0.7296942586417389 1.3831468193261922 7 0.5089763494594829 1.1255680201465188 8 0.3749338104188027 0.9475904941409717 9 0.3132221055702292 0.8905760609968455 >10 2.315304234178653 15.267320735158327 >50 0.4326301744993248 9.61249456866375 >100 0.3565309892415532 21.56628306595893 >500 0.019340124416405008 4.102012846982513 >1k 0.007567874771636742 4.7549771042038325 >5k 4.2043748731315236E-4 0.6869672779391106 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5172 0.6848317448379416 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4815 0.6375608761397311 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4525 0.5991615710347421 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3377 0.4471532873777512 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2537 0.33592771397019683 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2419 0.32030316913437373 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1732 0.22933653945462393 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1723 0.22814483688240014 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1450 0.19199652552494498 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1419 0.1878917722206186 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1419 0.1878917722206186 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1393 0.18444907590086096 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1330 0.17610715789529435 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1319 0.17465063252924304 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1294 0.17134034760639916 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1278 0.1692217652557791 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1272 0.16842729687429658 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1266 0.16763282849281402 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1231 0.1629984296008326 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 936 0.12393706751127483 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 923 0.12221571935139601 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 919 0.12168607376374099 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 852 0.11281451017051941 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 850 0.11254968737669188 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 840 0.11122557340755433 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 822 0.10884216826310673 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 799 0.10579670613409037 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 790 0.10460500356186658 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 760 0.10063266165445392 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.620569845687757E-5 0.0 20-21 0.0 0.0 0.0 1.9861709537063272E-4 0.0 22-23 0.0 0.0 0.0 7.944683814825309E-4 0.0 24-25 0.0 0.0 0.0 0.002118582350620083 0.0 26-27 0.0 0.0 0.0 0.0032440792243870016 0.0 28-29 0.0 0.0 0.0 0.00628954135340337 0.0 30-31 0.0 0.0 0.0 0.011387380134582945 0.0 32-33 0.0 0.0 0.0 0.022245114681510865 0.0 34-35 0.0 0.0 0.0 0.03542004867442951 0.0 36-37 0.0 0.0 0.0 0.0493232453503738 0.0 38-39 0.0 0.0 0.0 0.06501399588465379 0.0 40-41 0.0 0.0 0.0 0.07799031278220178 0.0 42-43 0.0 0.0 0.0 0.09129765817203418 0.0 44-45 0.0 0.0 0.0 0.10520085484797848 0.0 46-47 0.0 0.0 0.0 0.1267177068464637 0.0 48-49 0.0 0.0 0.0 0.15128002097396526 0.0 50-51 0.0 1.3241139691375515E-4 0.0 0.17140655330485605 0.0 52-53 0.0 1.3241139691375515E-4 0.0 0.20530387091477736 0.0 54-55 0.0 1.3241139691375515E-4 0.0 0.2781963449157996 0.0 56-57 0.0 1.3241139691375515E-4 0.0 0.4063705771283146 0.0 58-59 0.0 1.3241139691375515E-4 0.0 0.5207740240617991 0.0 60-61 0.0 1.3241139691375515E-4 0.0 0.6274314042758289 0.0 62-63 0.0 1.3241139691375515E-4 0.0 0.7516332945809312 0.0 64-65 0.0 1.3241139691375515E-4 0.0 0.9027809041579826 0.0 66-67 0.0 1.3241139691375515E-4 0.0 1.144299292128672 0.0 68-69 0.0 1.3241139691375515E-4 0.0 1.4256073048719449 0.0 70-71 0.0 1.3241139691375515E-4 0.0 1.6673243099380048 0.0 72-73 0.0 1.9861709537063272E-4 0.0 1.905267590192023 0.0 74-75 0.0 2.648227938275103E-4 0.0 2.1800874444865217 0.0 76-77 0.0 2.648227938275103E-4 0.0 2.4923135184091567 0.0 78-79 0.0 2.648227938275103E-4 0.0 2.7491254227233846 0.0 80-81 0.0 2.648227938275103E-4 0.0 3.021561871873436 0.0 82-83 0.0 2.648227938275103E-4 0.0 3.3019430048383125 0.0 84-85 0.0 2.648227938275103E-4 0.0 3.740158522924385 0.0 86-87 0.0 2.648227938275103E-4 0.0 4.317141184976073 0.0 88-89 0.0 2.648227938275103E-4 0.0 4.956886849164881 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 160 1.8189894E-12 35.630615 9 GTATGGT 100 1.2947985E-6 33.261852 1 CGCCCCT 30 0.003945732 31.675856 84-85 TAGGCAT 235 0.0 28.306082 5 CATGGGT 485 0.0 27.42882 4 GGGGAGC 175 4.947651E-9 27.147135 7 GTAAGCG 45 8.9250645E-4 26.40092 94-95 CTGGACC 90 8.933002E-4 26.394798 4 GTACATG 3620 0.0 25.071089 1 GGATATG 365 0.0 24.734842 1 GAGTACT 3695 0.0 24.491411 12-13 CATGGGG 1265 0.0 24.41258 4 ACATGGG 3635 0.0 24.310806 3 GGCATTC 240 2.2919266E-10 23.753744 7 TCGCCTG 50 0.0016600306 23.73487 32-33 GGATGAC 410 0.0 23.174385 8 GTAGGGT 540 0.0 22.87549 3 CTATACC 105 0.0021926723 22.624113 4 GTACTTT 3970 0.0 22.557827 14-15 TTAGGCA 320 0.0 22.27061 4 >>END_MODULE