##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936647_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 755222 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83973454163147 31.0 31.0 34.0 30.0 34.0 2 31.950065013995886 31.0 31.0 34.0 30.0 34.0 3 32.05209594000175 33.0 31.0 34.0 30.0 34.0 4 35.81178514397091 37.0 35.0 37.0 35.0 37.0 5 35.67042008839785 37.0 35.0 37.0 33.0 37.0 6 35.739589153917656 37.0 35.0 37.0 33.0 37.0 7 35.67049159055218 37.0 35.0 37.0 33.0 37.0 8 35.659996663232796 37.0 35.0 37.0 33.0 37.0 9 37.227797919022485 39.0 37.0 39.0 33.0 39.0 10-11 37.14613120380497 39.0 37.0 39.0 33.0 39.0 12-13 37.0594858200635 39.0 37.0 39.0 33.0 39.0 14-15 38.07289843251388 40.0 37.0 41.0 33.0 41.0 16-17 38.05416221985059 40.0 37.0 41.0 33.0 41.0 18-19 38.281233200304015 40.0 37.5 41.0 33.5 41.0 20-21 38.35282407027337 40.0 38.0 41.0 34.0 41.0 22-23 38.25616242641237 40.0 38.0 41.0 33.5 41.0 24-25 38.14690514841993 40.0 37.0 41.0 33.0 41.0 26-27 37.92738294170456 40.0 37.0 41.0 33.0 41.0 28-29 37.93785337291551 40.0 37.0 41.0 33.0 41.0 30-31 37.78764124985766 40.0 37.0 41.0 33.0 41.0 32-33 37.55216545598513 39.5 36.0 41.0 33.0 41.0 34-35 37.35854490467703 39.0 36.0 41.0 32.0 41.0 36-37 37.15854411020865 39.0 36.0 41.0 31.5 41.0 38-39 36.9518320440877 39.0 35.0 41.0 31.0 41.0 40-41 36.76578079028418 39.0 35.0 41.0 31.0 41.0 42-43 36.48049513917762 39.0 35.0 40.5 30.0 41.0 44-45 36.137820402477686 39.0 35.0 40.0 30.0 41.0 46-47 35.65827266684498 39.0 35.0 40.0 28.5 41.0 48-49 35.183455328367025 38.5 35.0 40.0 26.5 41.0 50-51 34.9087096244548 38.0 35.0 40.0 25.0 41.0 52-53 34.69818146187478 38.0 34.0 40.0 24.0 41.0 54-55 34.42170911334681 38.0 34.0 40.0 23.5 41.0 56-57 34.13204395528732 38.0 34.0 40.0 23.0 41.0 58-59 33.83602040194803 38.0 33.0 40.0 22.0 41.0 60-61 33.50083485385754 38.0 33.0 40.0 20.5 41.0 62-63 33.234895169897065 37.0 33.0 40.0 20.0 41.0 64-65 32.957909727206044 37.0 32.0 39.0 18.5 40.5 66-67 32.52638694317697 36.0 31.5 39.0 15.5 40.0 68-69 32.06980132994007 36.0 31.0 39.0 13.0 40.0 70-71 31.59103482154916 35.5 31.0 38.0 9.5 40.0 72-73 31.022941598629277 35.0 30.0 37.5 7.5 39.5 74-75 30.451160056248362 34.5 29.5 37.0 6.5 39.0 76-77 28.97468558913803 32.5 28.0 35.5 2.0 37.5 78-79 29.724450558908508 34.0 29.0 36.0 2.0 38.0 80-81 29.532876425739715 34.0 29.0 36.0 2.0 37.5 82-83 29.113131635466125 34.0 29.0 35.0 2.0 37.0 84-85 28.63088336939337 34.0 29.0 35.0 2.0 36.5 86-87 28.241201924732067 33.5 28.0 35.0 2.0 36.0 88-89 27.728122326944924 33.0 26.5 35.0 2.0 36.0 90-91 27.167657324601244 33.0 26.0 35.0 2.0 35.0 92-93 26.634308852231527 32.0 25.0 34.0 2.0 35.0 94-95 25.93228679778926 32.0 24.5 34.0 2.0 35.0 96-97 25.14482628948839 32.0 21.0 34.0 2.0 35.0 98-99 24.1641411399562 31.0 9.5 34.0 2.0 35.0 100-101 20.4110778022886 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3277294374887916 1101 2 0.46647941888659616 1101 3 0.3653815159368037 1101 4 0.30860460770352915 1101 5 0.2938770744665433 1101 6 0.3043224923531582 1101 7 0.4152863780714071 1101 8 0.21451232734927572 1101 9 0.46039290068056715 1101 10-11 0.4696908875478911 1101 12-13 0.4634316902201121 1101 14-15 0.35805516059149056 1101 16-17 0.5158095563959861 1101 18-19 0.38939447428285945 1101 20-21 0.33006559905740573 1101 22-23 0.31048320294927834 1101 24-25 0.47304653080606585 1101 26-27 0.288413681897687 1101 28-29 0.40770347098773385 1101 30-31 0.5402190877175315 1101 32-33 0.535983269483765 1101 34-35 0.3945773431160049 1101 36-37 0.3504629104354322 1101 38-39 0.3660059305085497 1101 40-41 0.6445612822703524 1101 42-43 0.7128224942518315 1101 44-45 0.551206930811432 1101 46-47 0.6031904592436561 1101 48-49 0.6740194821364724 1101 50-51 0.5581666844749336 1101 52-53 0.6295359419774869 1101 54-55 0.6671129470558199 1101 56-57 0.6326568131084898 1101 58-59 0.8204623792714756 1101 60-61 0.8049847816208242 1101 62-63 0.7808164532823341 1101 64-65 0.8502741486387606 1101 66-67 1.0471381273487879 1101 68-69 0.9700717706555224 1101 70-71 0.8069998398125406 1101 72-73 0.7121308611046615 1101 74-75 0.8066035248299386 1101 76-77 0.8383047253576201 1101 78-79 0.9781573822558123 1101 80-81 1.0122039878849414 1101 82-83 0.879847012915068 1101 84-85 0.8651758626498243 1101 86-87 0.9590535246299297 1101 88-89 1.2319007162558258 1101 90-91 0.9343565830960223 1101 92-93 1.0011356335165793 1101 94-95 1.1451267870222495 1101 96-97 1.1622779470314804 1101 98-99 0.4958393692147389 1101 100-101 1.2468568057450113 1102 1 0.1491087320270772 1102 2 0.1612087015831527 1102 3 0.12098036385074806 1102 4 0.20295801086489007 1102 5 -0.03450106645772877 1102 6 -0.08398398698492571 1102 7 0.1129508826667589 1102 8 0.08021179179733195 1102 9 0.10825642279404946 1102 10-11 0.23653785415420003 1102 12-13 0.12815631262161276 1102 14-15 0.19090489281551726 1102 16-17 0.36053845710113563 1102 18-19 0.22686783038287928 1102 20-21 0.1447592801557107 1102 22-23 0.09929314694934277 1102 24-25 -0.08664344497568521 1102 26-27 0.23973117022785573 1102 28-29 0.24846653700348043 1102 30-31 0.203128486997862 1102 32-33 0.05468022042843046 1102 34-35 0.04973766490556386 1102 36-37 0.1517662650838858 1102 38-39 0.06952407128002847 1102 40-41 0.16954810356713068 1102 42-43 0.14367746739783627 1102 44-45 0.12632019126080252 1102 46-47 0.09878457046426803 1102 48-49 0.3748415276240067 1102 50-51 0.4534949296828472 1102 52-53 0.6587827313397909 1102 54-55 0.5444027944884482 1102 56-57 0.4940038675728218 1102 58-59 0.3702541939598092 1102 60-61 0.383318229551584 1102 62-63 0.1667018711427275 1102 64-65 0.3677624788015592 1102 66-67 0.26225505491558465 1102 68-69 0.41456230872861255 1102 70-71 0.28424002836349516 1102 72-73 0.14700820366690692 1102 74-75 0.22130790168737136 1102 76-77 0.020992111237692512 1102 78-79 0.046055589188192414 1102 80-81 0.1910149250626354 1102 82-83 0.1283431127828969 1102 84-85 -0.014746669494751785 1102 86-87 0.09912365825052305 1102 88-89 -0.07433491131784109 1102 90-91 -0.46627828660671256 1102 92-93 -0.14856144730516618 1102 94-95 -0.06859052406390376 1102 96-97 0.06356831397241436 1102 98-99 -0.3016509473033331 1102 100-101 0.03557965570642807 1103 1 0.2647463822851037 1103 2 0.1874548002822003 1103 3 0.2555111261881322 1103 4 0.05372127528811177 1103 5 0.12299715671213818 1103 6 0.16062217475296592 1103 7 0.07287023002933068 1103 8 0.22788182671590818 1103 9 0.2293249929865624 1103 10-11 0.1959511911956966 1103 12-13 0.12278024266085907 1103 14-15 0.36411801972754176 1103 16-17 0.37410571398442727 1103 18-19 0.21254348935510592 1103 20-21 0.2809293614263737 1103 22-23 0.2856741302316763 1103 24-25 0.3767384035456942 1103 26-27 0.3064944037496886 1103 28-29 0.42905428151075853 1103 30-31 0.40274685478625116 1103 32-33 0.26623193391071 1103 34-35 0.24674409239543849 1103 36-37 0.30388996336267127 1103 38-39 0.2834482392599966 1103 40-41 0.21110816042570235 1103 42-43 0.3906900751664466 1103 44-45 0.36917887918762915 1103 46-47 0.40878426145033586 1103 48-49 0.4395598614208609 1103 50-51 0.44897096565207306 1103 52-53 0.31530910567926895 1103 54-55 0.44859070586325345 1103 56-57 0.275218910371251 1103 58-59 -0.07292355085648694 1103 60-61 -0.08695771989373924 1103 62-63 0.25799926615524527 1103 64-65 0.07838406033488354 1103 66-67 0.20852216656231803 1103 68-69 0.190661625807806 1103 70-71 0.0241337275693283 1103 72-73 0.3574490120473648 1103 74-75 0.23089351399848468 1103 76-77 0.06390642167309224 1103 78-79 0.179482399237326 1103 80-81 0.29454000154358795 1103 82-83 0.2853747249086034 1103 84-85 0.13343787362814297 1103 86-87 0.18476996089876963 1103 88-89 0.19145029309487782 1103 90-91 0.24730751111276206 1103 92-93 0.46645663001012494 1103 94-95 0.5736277324794656 1103 96-97 0.7783924850425272 1103 98-99 0.4320462499402673 1103 100-101 0.5964832124568353 1104 1 0.16890023613144933 1104 2 0.14602876463130698 1104 3 0.053724831530765016 1104 4 0.03728336434033963 1104 5 0.005269213513557247 1104 6 0.03532529233090287 1104 7 0.22849912075159295 1104 8 0.14119215947422958 1104 9 0.30178930459435094 1104 10-11 0.1483656265565898 1104 12-13 0.15214972689796724 1104 14-15 0.15179708670421377 1104 16-17 0.1270870385175371 1104 18-19 0.24740686093938535 1104 20-21 0.23876268225939867 1104 22-23 0.2646861055310623 1104 24-25 0.04970210263816455 1104 26-27 0.03947835334842864 1104 28-29 0.34109583254954146 1104 30-31 0.2294331469435491 1104 32-33 0.26107242992311086 1104 34-35 0.11825338012763353 1104 36-37 0.1389108388845628 1104 38-39 0.1442166334698527 1104 40-41 0.14094078124121978 1104 42-43 0.1579780073647541 1104 44-45 0.27057328904787425 1104 46-47 0.1354149272169778 1104 48-49 -0.1298043726849869 1104 50-51 0.12530974912166215 1104 52-53 0.25253253626504346 1104 54-55 -0.20722568954663245 1104 56-57 -0.05703627100828612 1104 58-59 -0.017100280274718216 1104 60-61 -0.18567268776794066 1104 62-63 -0.12271025158269566 1104 64-65 -0.043597114194476205 1104 66-67 0.20784402460876095 1104 68-69 0.18259579916213653 1104 70-71 0.09123853018939343 1104 72-73 0.17056308989931068 1104 74-75 0.27892283973163146 1104 76-77 0.12341415936653632 1104 78-79 0.19301086244890442 1104 80-81 0.13147654995729852 1104 82-83 0.15193701396583137 1104 84-85 0.20354900140634058 1104 86-87 0.060476228555462086 1104 88-89 -0.020664384772068445 1104 90-91 0.0959162263306439 1104 92-93 0.032683285676515794 1104 94-95 0.06845885325748569 1104 96-97 0.6615176881726086 1104 98-99 0.5042949311572933 1104 100-101 0.4472607318948221 1105 1 -0.0828672863444524 1105 2 0.1313056453126329 1105 3 0.061225112089886125 1105 4 0.03916934511268977 1105 5 -0.18788639081079594 1105 6 -0.0066432351701237735 1105 7 -0.03937561271723666 1105 8 0.09667989592322357 1105 9 0.1574634569940656 1105 10-11 -0.01019703788224291 1105 12-13 -0.056307260271132975 1105 14-15 0.03611164959310287 1105 16-17 0.16710412145081222 1105 18-19 0.09537498942300005 1105 20-21 0.2713340377963931 1105 22-23 0.2347145978686811 1105 24-25 0.14839500885660328 1105 26-27 0.20601201420856086 1105 28-29 0.019939804269178296 1105 30-31 0.1144272863751965 1105 32-33 0.2003532010242708 1105 34-35 -0.02146973919104056 1105 36-37 0.18612426332686738 1105 38-39 0.12598184912630472 1105 40-41 -0.19945225091709062 1105 42-43 0.10447382391649285 1105 44-45 -0.04893835103841582 1105 46-47 0.018462298066260985 1105 48-49 0.06648725917156639 1105 50-51 -0.0798335230705689 1105 52-53 -0.0655934534851994 1105 54-55 -0.1764000352204107 1105 56-57 -0.25487565402759316 1105 58-59 -0.06732036332022062 1105 60-61 0.03853145951936909 1105 62-63 -0.1272225774801754 1105 64-65 -0.3398550789562478 1105 66-67 -0.31317764702483686 1105 68-69 -0.3900082259260813 1105 70-71 -0.739797855211556 1105 72-73 -0.6243248111443691 1105 74-75 -0.2553755130745863 1105 76-77 -0.677522908104816 1105 78-79 -0.25019150172930793 1105 80-81 -0.11200512641463689 1105 82-83 -0.30869586506954505 1105 84-85 -0.00251388124513241 1105 86-87 0.020513698785020296 1105 88-89 0.13572550512406067 1105 90-91 -0.05650531063458075 1105 92-93 0.025787765893980463 1105 94-95 0.07738530935933241 1105 96-97 0.016771001887583736 1105 98-99 0.0371920470095084 1105 100-101 -0.20011074085353187 1106 1 0.07682106633611241 1106 2 0.17556501548968484 1106 3 0.14726404740579824 1106 4 0.23271614766949966 1106 5 0.16195943345105235 1106 6 0.0764167650200207 1106 7 0.12579049460682512 1106 8 0.13255863424912206 1106 9 0.13438780154911711 1106 10-11 -8.783583744573775E-4 1106 12-13 0.059711963367668375 1106 14-15 0.36177818632749137 1106 16-17 0.38541411451053165 1106 18-19 0.25960225682045746 1106 20-21 0.10334982615255228 1106 22-23 0.3715879519278005 1106 24-25 0.1921536644061419 1106 26-27 0.3168806907057089 1106 28-29 0.22961088546417585 1106 30-31 0.3249033138701378 1106 32-33 0.26978457301405 1106 34-35 0.07100163609457155 1106 36-37 0.1993575488070931 1106 38-39 0.12749429251860533 1106 40-41 0.3133722232533174 1106 42-43 0.6004578073865687 1106 44-45 0.8302594914519759 1106 46-47 0.479084103237561 1106 48-49 0.18421303234784148 1106 50-51 0.17760747658400078 1106 52-53 0.15704391820667496 1106 54-55 0.17183035055168716 1106 56-57 0.15317586887375256 1106 58-59 0.2356080249808059 1106 60-61 0.15504109827595158 1106 62-63 0.25529487622748803 1106 64-65 0.36198282894395106 1106 66-67 0.43923164598512443 1106 68-69 0.16059643452285144 1106 70-71 0.32413753992517513 1106 72-73 0.595637857094161 1106 74-75 0.4148587453131789 1106 76-77 6.451418652346774E-4 1106 78-79 0.05761806192007768 1106 80-81 0.1905699094751938 1106 82-83 0.4094097223352655 1106 84-85 0.17118972738617444 1106 86-87 0.3627402626467351 1106 88-89 0.16986521084330164 1106 90-91 0.5636139101520499 1106 92-93 0.39542290972267224 1106 94-95 0.4244573964202978 1106 96-97 0.7395707889254126 1106 98-99 0.9571434650143189 1106 100-101 1.0116487640094185 1107 1 0.059538305768072064 1107 2 0.09873543649124272 1107 3 0.15519788932289913 1107 4 0.12321971700582424 1107 5 0.0027470729505125746 1107 6 0.13830365300339054 1107 7 0.09637247736949917 1107 8 0.059984022218699806 1107 9 0.09187058775730605 1107 10-11 0.05055286516385138 1107 12-13 0.04136789524924467 1107 14-15 0.1823157856628015 1107 16-17 0.08969391023317286 1107 18-19 0.09116025305882403 1107 20-21 0.22881698809607798 1107 22-23 0.15368398565595953 1107 24-25 0.24380396519108416 1107 26-27 0.21329853430403034 1107 28-29 0.203315124690036 1107 30-31 0.35544998462624733 1107 32-33 0.2625282477670936 1107 34-35 0.06254200878553462 1107 36-37 0.14672708019275404 1107 38-39 0.19901652236796963 1107 40-41 0.1555165188875165 1107 42-43 0.28061070707678226 1107 44-45 0.3145678808122909 1107 46-47 0.2040139579487814 1107 48-49 0.40737485253897177 1107 50-51 0.01452312859845506 1107 52-53 -0.20430533798550243 1107 54-55 -0.01924720117082046 1107 56-57 0.021632964623499618 1107 58-59 0.009811598049552117 1107 60-61 -0.36170532873644134 1107 62-63 -0.3787162983348864 1107 64-65 0.04770597047921399 1107 66-67 0.007969972007771275 1107 68-69 -0.12074986818348421 1107 70-71 -0.0871175130509485 1107 72-73 0.41892609297057604 1107 74-75 -0.06303392630950455 1107 76-77 -0.21845401249659346 1107 78-79 0.2302395727092268 1107 80-81 0.3393184915585685 1107 82-83 0.4244822369039021 1107 84-85 0.4052364411463323 1107 86-87 0.6054429618406942 1107 88-89 0.6937000038802132 1107 90-91 0.6417441588777528 1107 92-93 0.39151684355485017 1107 94-95 0.41957035102586815 1107 96-97 0.5272727659758552 1107 98-99 0.9106309313616201 1107 100-101 0.3766168517571593 1108 1 0.025470182638176908 1108 2 0.19870018625556085 1108 3 -0.13723642637165057 1108 4 0.057680511365788334 1108 5 0.09563718239019892 1108 6 0.09395229832863805 1108 7 0.1055375386475248 1108 8 0.15221339468252637 1108 9 0.14977114930176327 1108 10-11 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58-59 -1.3360138371300323 2316 60-61 -1.0530697825313737 2316 62-63 -0.8573053582636447 2316 64-65 -0.7363333362885527 2316 66-67 -0.7981147309175007 2316 68-69 -0.7059006448063343 2316 70-71 -0.6989006905042388 2316 72-73 -0.5904345211065412 2316 74-75 -0.9704970740449035 2316 76-77 -1.1713211290976453 2316 78-79 -1.1819685427739302 2316 80-81 -1.2241574945823395 2316 82-83 -1.3525320526788676 2316 84-85 -1.118234094400563 2316 86-87 -1.319929900450223 2316 88-89 -1.2075791498543111 2316 90-91 -1.5758716749242616 2316 92-93 -1.29386063611825 2316 94-95 -1.904555123731285 2316 96-97 -1.7950925488046288 2316 98-99 -1.3492521759630058 2316 100-101 -1.314759753043532 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 4.0 5 17.0 6 44.0 7 120.0 8 307.0 9 580.0 10 1025.0 11 1708.0 12 2411.0 13 3354.0 14 4457.0 15 6339.0 16 8632.0 17 11470.0 18 9616.0 19 5568.0 20 4828.0 21 4927.0 22 5231.0 23 5824.0 24 6775.0 25 7946.0 26 9490.0 27 11629.0 28 14581.0 29 18066.0 30 21828.0 31 26860.0 32 33546.0 33 42588.0 34 55486.0 35 73387.0 36 106268.0 37 152982.0 38 93492.0 39 3834.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.36909962748778 24.190649982850932 16.37239569194179 20.067854697719508 2 14.442652343987945 24.048222510017663 40.1560716451747 21.353053500819687 3 18.057162984954463 28.770323130828128 32.877456056484995 20.295057827732414 4 11.654250825589433 20.70091020076322 41.05410623157817 26.59073274206918 5 10.543418672584886 40.60888633468488 36.7759030273497 12.07179196538053 6 25.748587832451914 41.953226998154186 18.3429508144625 13.955234354931397 7 23.762183519790895 36.31546262617168 23.84745657231459 16.074897281722833 8 23.521420125238183 35.84343770731236 21.61570230971622 19.01943985773324 9 24.366345260069224 18.819896666145848 24.240819255794985 32.57293881798994 10-11 22.129651943402074 29.144741546194364 29.298206355217403 19.427400155186156 12-13 24.33231553106239 27.99925055149347 29.060726514852586 18.607707402591554 14-15 21.776015815290542 29.499873215499882 28.63919514683499 20.084915822374587 16-17 18.18947424175213 32.139977092009346 30.224842590324354 19.445706075914167 18-19 17.571465742060592 30.929105957393 33.378243906120794 18.121184394425615 20-21 18.72316168730312 29.43855871888477 33.23356068520703 18.604718908605076 22-23 18.742733886109704 29.362051751944474 32.93505981054477 18.96015455140105 24-25 19.24774088558477 30.04699964314963 32.97714502491663 17.728114446348968 26-27 18.747264868028434 29.054599358199834 34.46931653645066 17.72881923732107 28-29 17.60034479973412 29.860398479385502 34.346701164294956 18.192555556585425 30-31 18.61245270402235 29.54076143627498 33.469695816105954 18.37709004359671 32-33 18.51689273515393 29.76874077634613 33.63809047109243 18.076276017407505 34-35 19.27363778947863 29.41729877101304 33.31367007165505 17.995393367853282 36-37 19.14093223907396 29.005818218412266 33.417547873847674 18.4357016686661 38-39 18.212256399459203 30.404962518919305 33.4377453881917 17.945035693429787 40-41 18.30667009170813 29.988400761630356 33.93935822844144 17.76557091822007 42-43 18.12758249244742 30.184830486536445 32.67435155067064 19.01323547034549 44-45 18.78041917297589 29.707916350791702 32.43098476848158 19.08067970775083 46-47 19.732409807976993 30.53473018529651 31.40361377184457 18.32924623488193 48-49 19.150520918851065 30.49606463948814 31.383242143844576 18.97017229781622 50-51 18.59007037730977 30.585462417986932 31.778367749581243 19.04609945512205 52-53 18.99614216585575 31.60795612913648 30.95105840510635 18.44484329990142 54-55 18.965164524579876 30.630550110133253 30.92456981163804 19.47971555364883 56-57 19.43549465727502 30.963306093315268 30.468099250601544 19.133099998808166 58-59 19.54347706626713 30.201394714174963 31.03990487395203 19.215223345605885 60-61 19.282627593599315 30.87795889965645 31.416094136590218 18.42331937015402 62-63 18.94918037128254 30.4365615317391 31.7506157146262 18.86364238235216 64-65 18.94115746515502 31.113132645305498 30.867767829004688 19.0779420605348 66-67 18.91439539042426 30.904760990478813 30.061552375132493 20.119291243964433 68-69 18.67760311012007 30.452972145569724 30.99705777980223 19.87236696450798 70-71 19.4812159605079 30.599690938294916 30.845391021320488 19.073702079876693 72-73 19.10677055919365 29.681403754087476 31.506474744886642 19.705350941832233 74-75 18.706139639404025 31.395857734685002 30.258227821281796 19.63977480462918 76-77 18.76532682213736 30.672579355200924 30.762487884196755 19.79960593846496 78-79 18.84764305974517 30.347376895920675 30.686172987947145 20.118807056387006 80-81 18.981998265371654 30.638568335749895 30.113545328751794 20.265888070126653 82-83 19.011649270555054 30.88086229099011 30.459647972881264 19.647840465573573 84-85 18.554944829403485 30.609584442858413 30.188969058650457 20.646501669087648 86-87 18.133048204815847 30.852604887349962 30.52614156233655 20.48820534549764 88-89 19.041146498164736 30.397813041244486 29.979647883220956 20.581392577369822 90-91 18.830003264189457 29.77073207228359 30.66166378318896 20.737600880337993 92-93 19.163451174394 30.39408208178517 30.555231872965766 19.88723487085506 94-95 18.991602424730342 29.608404460698768 30.50072959066124 20.899263523909653 96-97 18.794962004499347 29.7700275813305 30.16878503113658 21.26622538303358 98-99 18.650487577353953 30.257139206330397 29.46855425651157 21.62381895980408 100-101 18.12068539740192 30.22782359643623 29.84375899996226 21.807732006199586 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 781.0 1 508.0 2 242.0 3 256.0 4 245.5 5 278.0 6 407.5 7 679.0 8 997.5 9 1258.0 10 1261.0 11 1092.5 12 1046.5 13 1077.5 14 1179.5 15 1234.0 16 1300.0 17 1407.5 18 1529.5 19 1713.0 20 1919.0 21 2155.5 22 2513.0 23 2950.0 24 3360.0 25 4290.5 26 6389.0 27 9056.5 28 10166.5 29 11523.5 30 14379.5 31 17182.0 32 21343.0 33 26786.0 34 32523.0 35 36908.0 36 42865.5 37 48111.5 38 46591.0 39 43061.5 40 38622.0 41 33202.0 42 29074.5 43 26831.5 44 25824.0 45 25321.5 46 23472.0 47 23940.5 48 33103.0 49 30267.5 50 16609.5 51 10355.0 52 7287.0 53 5693.0 54 4639.0 55 3953.5 56 3269.5 57 2658.5 58 2205.0 59 1828.0 60 1422.0 61 1068.0 62 797.0 63 513.0 64 300.5 65 186.5 66 133.0 67 107.5 68 74.5 69 48.5 70 41.0 71 32.0 72 25.5 73 25.0 74 25.0 75 19.0 76 10.5 77 10.0 78 13.5 79 14.0 80 8.0 81 5.0 82 5.0 83 3.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010460500356186658 2 0.007415038227170288 3 0.006355747051860247 4 5.296455876550207E-4 5 0.005296455876550207 6 0.0 7 1.3241139691375518E-4 8 0.0027806393351888586 9 0.0 10-11 0.0 12-13 0.0 14-15 4.63439889198143E-4 16-17 0.0035751077166713894 18-19 0.002317199445990715 20-21 6.620569845687759E-4 22-23 5.296455876550207E-4 24-25 4.63439889198143E-4 26-27 3.310284922843879E-4 28-29 1.3241139691375518E-4 30-31 5.958512861118983E-4 32-33 0.0040385476058695325 34-35 8.606740799394086E-4 36-37 0.0010592911753100414 38-39 0.005428867273463961 40-41 0.0 42-43 0.0021847880490769604 44-45 0.0058923071626621045 46-47 0.0 48-49 0.002383405144447593 50-51 9.26879778396286E-4 52-53 7.282626830256534E-4 54-55 6.620569845687759E-5 56-57 0.010990145943841678 58-59 0.0017875538583356947 60-61 0.002846845033645736 62-63 2.6482279382751035E-4 64-65 0.0026482279382751034 66-67 0.0015227310645081844 68-69 0.0013241139691375517 70-71 0.004369576098153921 72-73 0.0024496108429044707 74-75 0.007348832528713411 76-77 0.0013241139691375517 78-79 0.006355747051860247 80-81 0.0022509937475338375 82-83 0.0032440792243870016 84-85 0.002515816541361348 86-87 9.26879778396286E-4 88-89 0.002383405144447593 90-91 0.0076136553225409215 92-93 0.0031778735259301236 94-95 5.296455876550207E-4 96-97 1.3241139691375518E-4 98-99 0.0011254968737669188 100-101 0.0013903196675944293 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 755222.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.547259264916782 #Duplication Level Percentage of deduplicated Percentage of total 1 73.85674420450754 21.822643694732243 2 12.600542388631148 7.4462298567091665 3 4.560190968799926 4.042234345579904 4 2.188616088971008 2.586704280487782 5 1.2425528425471803 1.8357015494550428 6 0.8211734691018907 1.4558055235814817 7 0.5693309333882158 1.1775518087451076 8 0.43931856212750087 1.0384527564057375 9 0.32853034549698495 0.8736454165312889 >10 2.5177018454199143 15.618332563008872 >50 0.46278594866711503 9.67277020358311 >100 0.38109143589552025 22.190433811012255 >500 0.02199467651222673 4.301691033675836 >1k 0.00852854803535322 4.5168153740785675 >5k 8.977418984582339E-4 1.4209877824136063 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5496 0.7277330374379984 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5218 0.6909226690959744 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4945 0.6547743577385192 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2767 0.3663823352603605 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2558 0.33870835330538573 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2362 0.3127557195102897 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1808 0.23939980562006935 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1725 0.22840965967622764 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1689 0.22364284938733245 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1586 0.21000447550521567 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1583 0.20960724131447442 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1545 0.20457560823175172 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1502 0.19888191816446024 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1467 0.19424751927247882 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1455 0.19265858250951376 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1435 0.19001035457123866 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1276 0.1689569424619516 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1179 0.15611303696131734 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1103 0.14604977079587195 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1065 0.14101813771314925 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1006 0.1332058652952377 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 891 0.11797855465015585 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 858 0.11360897855200193 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 857 0.11347656715508818 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 856 0.11334415575817443 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 855 0.11321174436126066 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 829 0.10976904804150303 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 812 0.1075180542939692 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 810 0.10725323150014168 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 785 0.1039429465772978 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 783 0.1036781237834703 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 768 0.10169195282976395 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 759 0.10050025025754017 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.620569845687757E-5 0.0 20-21 0.0 0.0 0.0 1.9861709537063272E-4 0.0 22-23 0.0 0.0 0.0 7.944683814825309E-4 0.0 24-25 0.0 0.0 0.0 0.0019861709537063272 0.0 26-27 0.0 0.0 0.0 0.0032440792243870016 0.0 28-29 0.0 0.0 0.0 0.006223335654946493 0.0 30-31 0.0 0.0 0.0 0.011254968737669188 0.0 32-33 0.0 0.0 0.0 0.022046497586140232 0.0 34-35 0.0 0.0 0.0 0.035353842975972626 0.0 36-37 0.0 0.0 0.0 0.049058422556546286 0.0 38-39 0.0 0.0 0.0 0.06448435029699875 0.0 40-41 0.0 0.0 0.0 0.07712963870226239 0.0 42-43 0.0 0.0 0.0 0.09010595559981038 0.0 44-45 0.0 0.0 0.0 0.1036781237834703 0.0 46-47 0.0 0.0 0.0 0.12499635868658487 0.0 48-49 0.0 0.0 0.0 0.15028693549711208 0.0 50-51 0.0 0.0 0.0 0.17054587922491665 0.0 52-53 0.0 0.0 0.0 0.20444319683483797 0.0 54-55 0.0 0.0 0.0 0.277997727820429 0.0 56-57 0.0 0.0 0.0 0.405642314445289 0.0 58-59 0.0 0.0 0.0 0.5201119670772303 0.0 60-61 0.0 0.0 0.0 0.6269679643866306 0.0 62-63 0.0 0.0 0.0 0.7507064148025349 0.0 64-65 0.0 0.0 0.0 0.9021188471734138 0.0 66-67 0.0 0.0 0.0 1.1434386180487328 0.0 68-69 0.0 0.0 0.0 1.4252762763796607 0.0 70-71 0.0 0.0 0.0 1.6665960472549792 0.0 72-73 0.0 0.0 0.0 1.9054662072873936 0.0 74-75 0.0 0.0 0.0 2.1794253875019534 0.0 76-77 0.0 0.0 0.0 2.4917176671230448 0.0 78-79 0.0 0.0 0.0 2.750052302501781 0.0 80-81 0.0 0.0 0.0 3.0216942832703495 0.0 82-83 0.0 0.0 0.0 3.302605061822881 0.0 84-85 0.0 0.0 0.0 3.735855152524688 0.0 86-87 0.0 0.0 0.0 4.3068130960168 0.0 88-89 0.0 0.0 0.0 4.938547870692326 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCGAG 20 0.002075137 71.23843 7 GACATAT 210 0.0 40.721157 1 TCGCCAA 25 0.0016170033 37.99509 22-23 CACCTAG 60 0.0039532436 31.661524 9 CATATAG 320 0.0 26.719719 3 GGATGAC 320 0.0 23.746141 8 GAGTACT 4460 0.0 23.695253 12-13 ACCCTTG 465 0.0 23.490808 7 CCCTTGG 470 0.0 23.240906 8 GTACATG 3640 0.0 23.231943 1 GGATATG 410 0.0 23.166967 9 GTACTTT 4700 0.0 22.538052 14-15 CCTTGGC 495 0.0 22.067123 9 ATGGGAT 800 0.0 21.968092 5 ATGGGCG 110 0.0028769623 21.590261 5 ACATGGG 3700 0.0 21.56835 3 CATTCCG 200 5.3894655E-7 21.37153 9 CATGGGA 1810 0.0 21.256268 4 ATAGGAC 135 3.793353E-4 21.111877 3 TTAGGCA 225 7.145718E-8 21.110477 4 >>END_MODULE