##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936646_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 770407 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.498391110153463 31.0 31.0 34.0 30.0 34.0 2 31.579674120302645 31.0 31.0 34.0 30.0 34.0 3 31.592027331008154 33.0 31.0 34.0 28.0 34.0 4 35.2572899778948 37.0 35.0 37.0 33.0 37.0 5 35.19271242343333 37.0 35.0 37.0 33.0 37.0 6 35.336281991207244 37.0 35.0 37.0 33.0 37.0 7 35.19085366565984 37.0 35.0 37.0 32.0 37.0 8 35.19958022188272 37.0 35.0 37.0 32.0 37.0 9 36.700127335291604 39.0 37.0 39.0 32.0 39.0 10-11 36.59997767413847 39.0 37.0 39.0 32.0 39.0 12-13 36.559936501096175 39.0 37.0 39.0 32.0 39.0 14-15 37.60214795556115 40.0 37.0 41.0 32.0 41.0 16-17 37.55079393100011 40.0 37.0 41.0 32.0 41.0 18-19 37.62655193942942 40.0 37.0 41.0 32.0 41.0 20-21 37.589351472663154 40.0 37.0 41.0 32.0 41.0 22-23 37.51338058974023 40.0 37.0 41.0 32.0 41.0 24-25 37.37773670280774 39.0 37.0 41.0 32.0 41.0 26-27 37.19272280755497 39.0 36.0 41.0 31.5 41.0 28-29 37.308528479102606 39.0 36.5 41.0 32.0 41.0 30-31 37.242412127615665 39.0 36.0 41.0 32.0 41.0 32-33 37.06755065828841 39.0 36.0 41.0 31.0 41.0 34-35 36.902587203906506 39.0 35.5 41.0 31.0 41.0 36-37 36.67768335438281 39.0 35.0 41.0 30.0 41.0 38-39 36.508527959896526 39.0 35.0 40.5 30.0 41.0 40-41 36.406761620805625 39.0 35.0 40.0 30.0 41.0 42-43 36.15275172733374 39.0 35.0 40.0 30.0 41.0 44-45 35.83346335118969 39.0 35.0 40.0 29.5 41.0 46-47 35.418412605285255 38.0 35.0 40.0 27.0 41.0 48-49 35.06364622855192 38.5 35.0 40.0 25.0 41.0 50-51 34.333801484150584 38.0 34.0 39.5 24.0 40.5 52-53 34.54339978738511 38.0 34.5 39.5 24.0 40.5 54-55 34.95768535332623 39.0 35.0 40.0 24.0 41.0 56-57 34.93777250206709 39.0 35.0 41.0 24.5 41.0 58-59 34.768499637204755 39.0 35.0 40.0 23.5 41.0 60-61 34.48524935521095 38.5 34.0 40.0 22.5 41.0 62-63 34.217727123455525 38.0 34.0 40.0 22.0 41.0 64-65 33.95673780222661 38.0 34.0 40.0 22.0 41.0 66-67 33.58419640527669 37.5 33.5 40.0 20.5 41.0 68-69 33.209545733618725 37.0 33.0 39.0 19.0 41.0 70-71 32.815923920732814 36.5 32.5 39.0 18.0 41.0 72-73 32.3877956716385 36.0 32.0 39.0 14.5 40.0 74-75 31.929406145063584 35.5 32.0 38.0 10.5 40.0 76-77 31.499450290560702 35.0 31.0 37.0 7.5 39.0 78-79 31.022935279663866 35.0 31.0 37.0 6.5 39.0 80-81 30.552528079313923 35.0 31.0 36.0 2.0 39.0 82-83 30.12183754820504 34.5 30.0 36.0 2.0 37.5 84-85 29.69193426331796 34.0 30.0 35.5 2.0 37.0 86-87 29.380823382965108 34.0 29.5 35.0 2.0 36.5 88-89 28.97770334381697 34.0 29.0 35.0 2.0 36.0 90-91 28.615586955985602 34.0 29.0 35.0 2.0 36.0 92-93 28.299984943023624 34.0 29.0 35.0 2.0 36.0 94-95 27.91676347696737 34.0 29.0 35.0 2.0 35.0 96-97 27.397138136076126 34.0 27.0 35.0 2.0 35.0 98-99 26.838846869252226 34.0 26.0 35.0 2.0 35.0 100-101 24.81776255927062 31.5 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21080584654057333 1101 2 0.29996731809213273 1101 3 0.2341643518583254 1101 4 0.2023078324862766 1101 5 0.24346929060430256 1101 6 0.15797199355702674 1101 7 0.27646547351533 1101 8 0.17635144922823542 1101 9 0.5623551427653695 1101 10-11 0.4235629824243645 1101 12-13 0.4966901872342788 1101 14-15 0.39804039499137644 1101 16-17 0.5565142329662365 1101 18-19 0.595943049730991 1101 20-21 0.5308861474333284 1101 22-23 0.44805203625696066 1101 24-25 0.3705118905744058 1101 26-27 0.3381719141858355 1101 28-29 0.46255234609252227 1101 30-31 0.3366877072456731 1101 32-33 0.3906178419990951 1101 34-35 0.4336222855982754 1101 36-37 0.2706362280942116 1101 38-39 0.4138040158088785 1101 40-41 0.3516098748235734 1101 42-43 0.31389715122374184 1101 44-45 0.5572424928075179 1101 46-47 0.519812787033004 1101 48-49 0.5728284373236079 1101 50-51 0.5835091292688688 1101 52-53 0.661608101031689 1101 54-55 0.8730047615956167 1101 56-57 0.6116468702294497 1101 58-59 0.6320463789478836 1101 60-61 0.44804385955268344 1101 62-63 0.6365573231814281 1101 64-65 0.6931011953352737 1101 66-67 0.5711391254076403 1101 68-69 0.6574713962565255 1101 70-71 0.5921118993745935 1101 72-73 0.6199944740272301 1101 74-75 0.6025459186972135 1101 76-77 0.5440293466739092 1101 78-79 0.5162063239343873 1101 80-81 0.37757023102210496 1101 82-83 0.3193418383269844 1101 84-85 0.03668717589521009 1101 86-87 0.3983474340943296 1101 88-89 0.3647400495843094 1101 90-91 0.5602602396958503 1101 92-93 0.20594870529133757 1101 94-95 -0.013562748527103707 1101 96-97 -0.12864486051827484 1101 98-99 -0.031851271313065865 1101 100-101 0.41216892053234844 1102 1 0.3118126516694453 1102 2 0.3175926295214886 1102 3 0.36474623047208254 1102 4 0.2477384967675036 1102 5 0.4818299209566703 1102 6 0.35334223846977153 1102 7 0.20217730216213425 1102 8 0.17614406009155914 1102 9 0.4714371278388825 1102 10-11 0.4825465267876652 1102 12-13 0.5633588817058452 1102 14-15 0.48574526089355885 1102 16-17 0.3917539333304063 1102 18-19 0.40729566933231354 1102 20-21 0.34243163893176387 1102 22-23 -0.004471059979891834 1102 24-25 0.14012606382452475 1102 26-27 0.28298151713242703 1102 28-29 0.5635715945695949 1102 30-31 0.2603726527299841 1102 32-33 0.3378663411930418 1102 34-35 0.2528722835243826 1102 36-37 0.1817374920618633 1102 38-39 0.15334676424033233 1102 40-41 0.05947185873570504 1102 42-43 0.4237850504823939 1102 44-45 0.30893755335132056 1102 46-47 0.7036998954477482 1102 48-49 0.7493801919739553 1102 50-51 0.6023227804513951 1102 52-53 0.7272730320817189 1102 54-55 0.6287618840298634 1102 56-57 0.8674302619543468 1102 58-59 0.8327956067689968 1102 60-61 0.48860018782480097 1102 62-63 0.8333606270286325 1102 64-65 1.0006724196777839 1102 66-67 0.7651475149895148 1102 68-69 0.7743842568716701 1102 70-71 0.9022499099320811 1102 72-73 0.6929187161581964 1102 74-75 0.793977843904397 1102 76-77 0.7034182676697043 1102 78-79 0.8625931903951418 1102 80-81 0.7844739388633499 1102 82-83 0.7746045450593897 1102 84-85 0.5032830076500829 1102 86-87 0.6384121118530857 1102 88-89 0.3361645917382212 1102 90-91 0.3423799778878056 1102 92-93 0.3441065090680304 1102 94-95 0.5368535505999255 1102 96-97 0.36344531780872913 1102 98-99 0.20730227683854352 1102 100-101 0.44212835521721416 1103 1 0.2955667361158447 1103 2 0.32338455620095985 1103 3 0.4559608485052138 1103 4 0.22081920117768306 1103 5 0.4060316456454416 1103 6 0.11631476932508633 1103 7 0.15253456695766943 1103 8 -0.09519618680577224 1103 9 0.22232257685717371 1103 10-11 0.22552155321744038 1103 12-13 0.26582195885885795 1103 14-15 0.32199032630641256 1103 16-17 0.5549411932856287 1103 18-19 0.3124720666826164 1103 20-21 0.40605576153851075 1103 22-23 0.33099242441796406 1103 24-25 0.32656330218342333 1103 26-27 0.36541089031815943 1103 28-29 0.4331702869982621 1103 30-31 0.16867811652975462 1103 32-33 0.12593310740844998 1103 34-35 0.12340613078151108 1103 36-37 0.3498610531643678 1103 38-39 0.2869676371154881 1103 40-41 0.2390812327715821 1103 42-43 0.678045695410141 1103 44-45 0.1464091524797837 1103 46-47 0.1775217306273973 1103 48-49 0.4203380126518539 1103 50-51 -0.12558624351845538 1103 52-53 0.32005355453434703 1103 54-55 0.6310846879586123 1103 56-57 0.5085515701838403 1103 58-59 0.21637431540519714 1103 60-61 0.37130064621521086 1103 62-63 0.41282547713647233 1103 64-65 0.5592361480111947 1103 66-67 0.4089810464604895 1103 68-69 0.4387363467781711 1103 70-71 0.5766514862575818 1103 72-73 0.3704616996860324 1103 74-75 0.3376030908908483 1103 76-77 0.4655221466425701 1103 78-79 0.6191526433432806 1103 80-81 0.7268193126578986 1103 82-83 0.7345704571575773 1103 84-85 0.41191344460871804 1103 86-87 0.08812148712304335 1103 88-89 0.202900915098855 1103 90-91 0.1954633149919225 1103 92-93 0.09517775497672787 1103 94-95 0.14466168205911245 1103 96-97 0.3638333659266948 1103 98-99 0.30678451369598747 1103 100-101 0.4304704573410554 1104 1 0.07492682909403214 1104 2 -0.020462814082108594 1104 3 0.03341164920595929 1104 4 9.792134535260288E-4 1104 5 0.15124100995824818 1104 6 0.017143247020406704 1104 7 0.18113985153996737 1104 8 0.13379953795947586 1104 9 0.24285478604104327 1104 10-11 0.3659691954446558 1104 12-13 0.08045593539549856 1104 14-15 0.14909409274702767 1104 16-17 -0.1659807456732807 1104 18-19 -0.0619633366372625 1104 20-21 -0.36619243793266065 1104 22-23 -0.07633415435703483 1104 24-25 -0.005403916754339377 1104 26-27 0.07604257081646892 1104 28-29 -0.09219695836667796 1104 30-31 -0.11000850975732135 1104 32-33 -0.26019632038640594 1104 34-35 -0.3167903112115553 1104 36-37 -0.12862353311538754 1104 38-39 -0.09595074696228068 1104 40-41 -0.2656607399743294 1104 42-43 -0.468733812447681 1104 44-45 -0.4497594756230967 1104 46-47 -0.41894882083384033 1104 48-49 -0.29774939877804485 1104 50-51 -0.3475979481990521 1104 52-53 -0.17283313114283771 1104 54-55 -0.09932474853162887 1104 56-57 -0.11334661131400736 1104 58-59 -0.02006932580471954 1104 60-61 -0.15164339779597924 1104 62-63 -0.28196268633121946 1104 64-65 -0.47440379737264493 1104 66-67 -0.4189972321074933 1104 68-69 -0.0620394186508193 1104 70-71 -0.11861762335977488 1104 72-73 -0.39549867944445793 1104 74-75 -0.42422058302624066 1104 76-77 -0.6282119135801238 1104 78-79 -0.4498833757581657 1104 80-81 -0.03560607675219174 1104 82-83 0.0388182701668498 1104 84-85 -0.038596699305713855 1104 86-87 -0.04203447582776221 1104 88-89 -0.06844648715256696 1104 90-91 -0.3387982373061753 1104 92-93 -0.28438750380356126 1104 94-95 -0.23324619508578692 1104 96-97 0.016627897490828047 1104 98-99 -0.3784863151084963 1104 100-101 -0.25472648904161943 1105 1 0.15775754883669535 1105 2 0.03578713244835896 1105 3 0.22795376466022432 1105 4 0.1776311367882002 1105 5 0.19417205279945904 1105 6 0.13796727665535968 1105 7 0.0733467030543693 1105 8 -0.1551540847081867 1105 9 0.029300331244897393 1105 10-11 0.20952233845498114 1105 12-13 0.00521289855428364 1105 14-15 -0.023399326936036857 1105 16-17 0.16587896738782604 1105 18-19 -0.13272420093301918 1105 20-21 -0.2989726824381336 1105 22-23 -0.513453273065096 1105 24-25 -0.5444238416403451 1105 26-27 -0.5876126918686424 1105 28-29 -0.3939625935704498 1105 30-31 -0.4870307274168155 1105 32-33 -0.56959312704344 1105 34-35 -0.6347657694097819 1105 36-37 -0.5973901977858631 1105 38-39 -0.799339574539438 1105 40-41 -1.003193784144436 1105 42-43 -0.9537238457271116 1105 44-45 -0.9080040495351014 1105 46-47 -0.8599441240702461 1105 48-49 -0.6967354738863349 1105 50-51 -0.7034020301343347 1105 52-53 -0.5166963974325043 1105 54-55 -0.4140706470621396 1105 56-57 -0.5175513126403359 1105 58-59 -0.5785450306757198 1105 60-61 -0.5162621101356066 1105 62-63 -0.6381460454725172 1105 64-65 -0.6161871702021386 1105 66-67 -0.9255155243904838 1105 68-69 -0.789391864133286 1105 70-71 -0.8721744165567458 1105 72-73 -0.9629864955206742 1105 74-75 -1.1637063344656404 1105 76-77 -0.8919906552353147 1105 78-79 -0.8393989275494711 1105 80-81 -0.43152300078553196 1105 82-83 -0.613262621391236 1105 84-85 -0.8115451090505736 1105 86-87 -0.7232377256345615 1105 88-89 -1.1053048171351456 1105 90-91 -0.9929059805497644 1105 92-93 -1.0999746562872552 1105 94-95 -1.0092010183226456 1105 96-97 -0.8572068231985988 1105 98-99 -0.9242006252659962 1105 100-101 -0.628754412969446 1106 1 -0.18790673119512746 1106 2 -0.046695644849833684 1106 3 -0.12233426774836076 1106 4 0.12936807659196603 1106 5 0.16793881707554448 1106 6 0.1745877340090729 1106 7 0.4337906284198425 1106 8 0.2783248164241954 1106 9 0.3439355372120474 1106 10-11 0.14382358480873592 1106 12-13 0.17603360754845454 1106 14-15 0.025770710922877527 1106 16-17 0.15314601659312643 1106 18-19 0.0773899400194864 1106 20-21 0.2564954195947351 1106 22-23 0.25047863731479225 1106 24-25 0.17485035054300369 1106 26-27 0.07291389118117308 1106 28-29 0.025334731480199935 1106 30-31 -0.05488055561296079 1106 32-33 0.058086194994288576 1106 34-35 -0.1465014679377461 1106 36-37 -0.15317002931087842 1106 38-39 0.11525923947795036 1106 40-41 -0.01931626421349364 1106 42-43 0.07989639158225259 1106 44-45 -0.09240121265566614 1106 46-47 -0.031172479323380742 1106 48-49 -0.11267148047637932 1106 50-51 0.11062946441816024 1106 52-53 0.07848829876523666 1106 54-55 0.07391859897564501 1106 56-57 0.04577773264929874 1106 58-59 -0.0598863984488176 1106 60-61 -0.10469736913908889 1106 62-63 0.09944401755044652 1106 64-65 -0.06983680317945584 1106 66-67 -0.11839567921143157 1106 68-69 -0.27564755566496757 1106 70-71 -0.014348454709050884 1106 72-73 0.3463690566495856 1106 74-75 -0.04632113837227081 1106 76-77 -0.054570985527519156 1106 78-79 0.1073066136185723 1106 80-81 0.06849251516203836 1106 82-83 0.21789001603774238 1106 84-85 0.28084530604333935 1106 86-87 0.32419593663686186 1106 88-89 0.3327089723106269 1106 90-91 0.49102231884596037 1106 92-93 0.32147515385397085 1106 94-95 0.36449211572949736 1106 96-97 0.23396065311841951 1106 98-99 0.20791907885898553 1106 100-101 0.7404757703444211 1107 1 0.32984618627186535 1107 2 0.4088849285501581 1107 3 0.5521668850604087 1107 4 0.23813718739326362 1107 5 0.27396346659129733 1107 6 0.3955033646106614 1107 7 0.42787805284588387 1107 8 0.538532153216515 1107 9 0.6692115868081459 1107 10-11 0.6352256230691538 1107 12-13 0.6357004913865154 1107 14-15 0.739317142279269 1107 16-17 0.6075940355511733 1107 18-19 0.8316526318870388 1107 20-21 0.8681127951065761 1107 22-23 0.7730242348680676 1107 24-25 0.9411660547353122 1107 26-27 0.914855032107404 1107 28-29 0.7546567516467491 1107 30-31 0.7629191155917994 1107 32-33 0.6035580026592058 1107 34-35 0.47644398378438524 1107 36-37 0.6559962350823554 1107 38-39 0.7404056613823329 1107 40-41 0.9142427923399552 1107 42-43 1.113576805310629 1107 44-45 0.9800881791406582 1107 46-47 1.0677395257537015 1107 48-49 1.1783426874252285 1107 50-51 0.8986164543888933 1107 52-53 0.8370525032934282 1107 54-55 0.8894059012005826 1107 56-57 0.7979002173150889 1107 58-59 1.002677529270251 1107 60-61 1.1126070837746624 1107 62-63 1.1057481767764514 1107 64-65 0.8786826055292352 1107 66-67 1.0387584865972386 1107 68-69 0.9759837615751792 1107 70-71 1.0097710728250249 1107 72-73 1.3495759917310934 1107 74-75 1.0126130451926905 1107 76-77 1.3093562271732964 1107 78-79 1.4766645177008364 1107 80-81 1.5009924392083178 1107 82-83 1.5922397677534548 1107 84-85 1.6382109607813327 1107 86-87 1.323291243371763 1107 88-89 1.4983412617584584 1107 90-91 1.325505647847116 1107 92-93 1.7413119039797174 1107 94-95 1.6746786918368173 1107 96-97 1.628802720004959 1107 98-99 1.872222178108565 1107 100-101 1.6446137293159069 1108 1 -0.053656199542036376 1108 2 -0.06441431910667816 1108 3 -0.05148897802450847 1108 4 -0.11506233489359374 1108 5 0.23085187360876347 1108 6 0.13741535458675713 1108 7 0.39401802426381494 1108 8 0.21760028237518014 1108 9 0.339420742242865 1108 10-11 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60-61 -2.3423036708508107 2316 62-63 -2.427677115836616 2316 64-65 -2.33905434790163 2316 66-67 -3.2429946654361643 2316 68-69 -2.735627081014016 2316 70-71 -2.3929054585643854 2316 72-73 -2.100624001261316 2316 74-75 -2.3287756382835916 2316 76-77 -2.7023953204903215 2316 78-79 -2.7342326569486524 2316 80-81 -2.8253419077670827 2316 82-83 -2.7025770113727745 2316 84-85 -2.792896109694759 2316 86-87 -2.862383209713329 2316 88-89 -3.014547175530044 2316 90-91 -2.882570048901176 2316 92-93 -3.1528070664943506 2316 94-95 -3.575574411327846 2316 96-97 -4.088144035203246 2316 98-99 -4.173615773166539 2316 100-101 -5.066942712228787 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1320.0 3 440.0 4 371.0 5 440.0 6 477.0 7 515.0 8 682.0 9 948.0 10 1233.0 11 1741.0 12 2598.0 13 3512.0 14 4145.0 15 5892.0 16 7738.0 17 10430.0 18 8721.0 19 4807.0 20 4578.0 21 4566.0 22 5036.0 23 5625.0 24 6271.0 25 7445.0 26 8530.0 27 10283.0 28 12314.0 29 14907.0 30 18329.0 31 22594.0 32 28666.0 33 37057.0 34 49327.0 35 66824.0 36 94259.0 37 141565.0 38 148741.0 39 27478.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.163184234611414 23.26939678954167 15.921173057075535 20.64624591877138 2 14.250795410439576 22.996870282219803 41.02539471959105 21.726939587749573 3 17.699204648270722 28.769093569035416 32.95809312897627 20.57360865371759 4 11.111096683719843 19.864699924689017 42.02209987794427 27.00210351364687 5 10.564200591380187 39.99750669750397 37.03385307435885 12.404439636756992 6 25.191757729401466 41.38992859573341 19.270320604897258 14.147993069967871 7 23.383669796953328 35.91637161994607 24.567232351402268 16.13272623169834 8 22.92398313765763 35.4509066689863 22.31598845550136 19.309121737854714 9 24.217546128072012 18.72852300594183 24.86768159459744 32.18624927138872 10-11 21.844618903814826 28.650049379172966 29.92059725099878 19.58473446601343 12-13 23.920682307201858 27.739247077950324 29.473972624085665 18.866097990762153 14-15 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32.98068366870749 18.568432749168082 40-41 17.950756577623682 29.869791024394548 33.96148898526306 18.217963412718706 42-43 18.044410580450208 29.821898177834427 32.69312644567381 19.44056479604155 44-45 18.554856346233226 29.64794637549098 32.31989086103994 19.477306417235855 46-47 19.541564498605236 30.067460126872287 31.613058992887932 18.77791638163454 48-49 18.84187961243686 30.13355988128299 31.62122186620388 19.403338640076274 50-51 18.25717523393904 30.273704459931487 32.13385895250865 19.335261353620815 52-53 18.56809356703892 31.34646375030098 31.167725316540313 18.917717366119785 54-55 18.690663170601905 30.32509508171186 31.27604200469892 19.70819974298732 56-57 19.067995166103536 30.8937775914371 30.54830456663028 19.489922675829092 58-59 19.260005399904465 29.957527674510377 31.340798874327607 19.441668051257555 60-61 19.270156938259873 30.502330936521815 31.591896920879257 18.635615204339054 62-63 18.740646032230217 30.348262904094653 31.854503209392583 19.056587854282544 64-65 18.72244866169942 30.77383888470625 31.226667358965702 19.277045094628626 66-67 18.597430379056732 30.609266004314723 30.364778176135083 20.428525440493466 68-69 18.40424737865212 30.241933128450103 31.351547820330172 20.002271672567606 70-71 19.29966559872886 30.242674587061163 31.1028231983093 19.354836615900677 72-73 18.89924965065692 29.451260393483945 31.782788760531407 19.866701195327728 74-75 18.314880426592175 31.17923582315065 30.672021568450557 19.833862181806616 76-77 18.39376102940938 30.365525741400223 31.257419194466134 19.983294034724263 78-79 18.630282129056383 30.0037395603939 31.23318496281215 20.132793347737564 80-81 18.746884055713085 30.336314253367817 30.69315478972569 20.22364690119341 82-83 18.86561619350375 30.475815276297634 31.145547085021345 19.51302144517727 84-85 18.59061774871404 30.200918428610834 30.714070131598163 20.49439369107696 86-87 18.146007943158192 30.358402722923454 31.32173295592839 20.173856377989964 88-89 18.947855542048327 29.877415967272846 30.731616451585896 20.443112039092927 90-91 18.76268156627648 29.235205001616233 31.466122124700284 20.53599130740701 92-93 19.277005203194616 29.681901502798922 31.527945101623274 19.513148192383188 94-95 18.819408295698974 29.094010392665158 31.597674306906853 20.48890700472901 96-97 18.702442177736124 29.12602644416118 31.33565600263767 20.835875375465033 98-99 18.50929096544897 29.571528262386604 30.736688359110016 21.182492413054415 100-101 18.288917961686668 29.321611873821173 31.309606900424946 21.07986326406721 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 442.0 1 325.0 2 250.0 3 304.0 4 272.5 5 267.0 6 417.0 7 774.5 8 1254.0 9 1537.0 10 1380.5 11 1131.0 12 1072.0 13 1102.0 14 1177.5 15 1261.5 16 1354.0 17 1398.0 18 1514.5 19 1704.0 20 1955.0 21 2249.0 22 2533.5 23 2926.5 24 3355.5 25 4268.5 26 6406.5 27 9061.0 28 10175.5 29 11416.5 30 14371.5 31 17542.0 32 21682.0 33 27408.5 34 33685.5 35 38286.5 36 44330.5 37 49527.5 38 47459.0 39 43344.0 40 38807.0 41 34162.0 42 30564.5 43 27685.0 44 26339.5 45 26003.0 46 24064.5 47 24229.0 48 32938.0 49 30194.0 50 16699.5 51 10201.0 52 7254.5 53 5872.0 54 4974.5 55 4185.5 56 3399.5 57 2763.5 58 2316.0 59 1922.0 60 1458.0 61 1130.5 62 843.5 63 544.0 64 320.5 65 219.5 66 151.0 67 103.0 68 70.5 69 43.0 70 41.0 71 31.5 72 25.0 73 23.0 74 23.5 75 24.0 76 18.5 77 13.5 78 14.5 79 13.0 80 8.5 81 4.5 82 3.0 83 2.0 84 0.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01453777029544124 2 0.006490076024750554 3 0.00726888514772062 4 0.034657005972167955 5 0.04465172305028381 6 0.055165646210379705 7 0.024662288894052108 8 0.022066258484151884 9 0.013758961172471175 10-11 0.04484642533102633 12-13 0.04075767743543347 14-15 0.01888612123202411 16-17 0.021871556203409366 18-19 0.029919250474100056 20-21 0.022325861525141905 22-23 0.012266243686778547 24-25 0.002531129649652716 26-27 0.0028556334508902438 28-29 0.0012331144447026053 30-31 0.006100671463265521 32-33 0.0014278167254451219 34-35 0.0020768243279201772 36-37 0.006230472983760531 38-39 0.006295373744008037 40-41 0.0038940456148503326 42-43 6.490076024750554E-4 44-45 0.001687419766435144 46-47 0.003374839532870288 48-49 4.5430532173253873E-4 50-51 0.001817221286930155 52-53 9.735114037125831E-4 54-55 0.002206625848415188 56-57 0.001817221286930155 58-59 0.0029854349713852547 60-61 0.001103312924207594 62-63 0.0015576182459401328 64-65 0.0035046410533652993 66-67 0.002725831930395233 68-69 0.006425175264503048 70-71 0.009735114037125831 72-73 0.004218549416087861 74-75 0.002920534211137749 76-77 0.0030503357316327604 78-79 0.03413779989018791 80-81 0.02271526608662694 82-83 0.0031152364918802656 84-85 0.00902120567440327 86-87 0.007593388948958149 88-89 0.004867557018562916 90-91 0.012460945967521063 92-93 0.01440796877494623 94-95 0.00726888514772062 96-97 0.0037642440943553212 98-99 0.022001357723904377 100-101 0.04017357059320593 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 770407.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.60362942496844 #Duplication Level Percentage of deduplicated Percentage of total 1 75.78797637290981 23.95175120157705 2 11.90449534846844 7.524505189685144 3 4.181645089892899 3.9646548542314206 4 1.912415056178806 2.4175702696882073 5 1.1141536538128283 1.7605649598787596 6 0.7614239850935498 1.4438256876107545 7 0.5274769544680485 1.1669130339453408 8 0.38947894950009093 0.9847158711061497 9 0.2966339133552141 0.8437237445300743 >10 2.320780345836181 15.391381830900505 >50 0.4221676852295149 9.408477189146462 >100 0.35084443363419654 21.149203947639403 >500 0.022675022150271222 4.633880226699085 >1k 0.007420916340088763 4.6758346603556875 >5k 4.1227313000493125E-4 0.6829973330059497 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5242 0.680419570434848 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4795 0.6223982907735781 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4476 0.5809916057356695 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3388 0.43976755143709756 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2680 0.3478680749266297 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2551 0.33112367878277327 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1724 0.22377782133339907 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1660 0.2154705240217184 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1460 0.18951021992271616 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1457 0.18912081536123113 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1431 0.18574597582836086 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1367 0.17743867851668013 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1355 0.17588106027074 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1341 0.17406383898380984 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1333 0.17302542681984978 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1289 0.16731415991806928 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1235 0.16030487781133868 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1174 0.152386985061143 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1171 0.15199758049965797 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 918 0.11915779581442017 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 917 0.11902799429392516 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 873 0.11331672739214467 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 865 0.11227831522818459 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 864 0.11214851370768958 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 810 0.10513923160095898 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 800 0.10384121639600886 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.2980152049501108E-4 0.0 10-11 0.0 0.0 0.0 1.2980152049501108E-4 0.0 12-13 0.0 0.0 0.0 1.2980152049501108E-4 0.0 14-15 0.0 0.0 0.0 1.2980152049501108E-4 0.0 16-17 0.0 0.0 0.0 1.2980152049501108E-4 0.0 18-19 0.0 0.0 0.0 1.2980152049501108E-4 0.0 20-21 0.0 0.0 0.0 1.9470228074251663E-4 0.0 22-23 0.0 0.0 0.0 3.8940456148503326E-4 0.0 24-25 0.0 0.0 0.0 0.0012331144447026053 0.0 26-27 0.0 0.0 0.0 0.002206625848415188 0.0 28-29 0.0 0.0 0.0 0.005516564621037971 0.0 30-31 0.0 0.0 0.0 0.013045052809748613 0.0 32-33 0.0 0.0 0.0 0.023623876730092017 0.0 34-35 0.0 0.0 0.0 0.03731793714231568 0.0 36-37 0.0 0.0 0.0 0.05081729527379684 0.0 38-39 0.0 0.0 0.0 0.061590821474882755 0.0 40-41 0.0 0.0 0.0 0.07119613399151357 0.0 42-43 0.0 0.0 0.0 0.08151535487086695 0.0 44-45 0.0 0.0 0.0 0.09170477422972534 0.0 46-47 0.0 0.0 0.0 0.11117500230397699 0.0 48-49 0.0 0.0 0.0 0.1333710623086239 0.0 50-51 0.0 0.0 0.0 0.15297109190337055 0.0 52-53 0.0 0.0 0.0 0.18327974693895566 0.0 54-55 0.0 0.0 0.0 0.24597388133804599 0.0 56-57 0.0 0.0 0.0 0.3532548380271726 0.0 58-59 0.0 0.0 0.0 0.4628722220852095 0.0 60-61 0.0 0.0 0.0 0.5644419118725557 0.0 62-63 0.0 0.0 0.0 0.6826261962832633 0.0 64-65 0.0 0.0 0.0 0.8361813950288614 0.0 66-67 0.0 0.0 0.0 1.0700837349608714 0.0 68-69 0.0 0.0 0.0 1.34720998121772 0.0 70-71 0.0 0.0 0.0 1.5803984127870074 0.0 72-73 0.0 0.0 0.0 1.8091086918992167 0.0 74-75 0.0 0.0 0.0 2.077408434762405 0.0 76-77 0.0 0.0 0.0 2.379002267632563 0.0 78-79 0.0 0.0 0.0 2.631076820433875 0.0 80-81 0.0 0.0 0.0 2.889576548499689 0.0 82-83 0.0 0.0 0.0 3.160342520252282 0.0 84-85 0.0 0.0 0.0 3.5919974766584417 0.0 86-87 0.0 0.0 0.0 4.1589705181806504 0.0 88-89 0.0 0.0 0.0 4.797139693694372 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTAGC 220 0.0 36.727383 1 CATGGGG 1045 0.0 31.381117 4 CATTCCG 170 1.07320375E-10 30.746428 9 GACATAT 285 0.0 28.350964 1 ACTGCGA 70 0.008375069 27.152689 8 CTTTGCG 70 0.008375069 27.152689 9 GGGTATG 145 7.8570156E-7 26.216393 7 ATGGGGA 610 0.0 25.71104 5 CATATAG 315 0.0 25.649208 3 GACAATG 380 0.0 25.009058 7 GTACATG 3695 0.0 24.440098 1 GAGTACT 3870 0.0 23.94278 12-13 ACATGGG 3730 0.0 23.826944 3 CATGGGT 455 0.0 22.979832 4 GGCATTC 270 4.0017767E-11 22.878656 7 GTACTTT 4175 0.0 22.020086 14-15 TACATGG 4135 0.0 21.608164 2 GGATATG 425 0.0 21.248508 1 TTAGGCA 295 1.3278623E-10 20.943869 4 TACACTG 160 4.965189E-5 20.792831 5 >>END_MODULE