##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936646_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 770407 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.416639516515296 31.0 31.0 34.0 30.0 34.0 2 31.636111821413877 31.0 31.0 34.0 30.0 34.0 3 31.885644860443897 31.0 31.0 34.0 30.0 34.0 4 35.712958215592536 37.0 35.0 37.0 33.0 37.0 5 35.51036270438872 37.0 35.0 37.0 33.0 37.0 6 35.63128969492749 37.0 35.0 37.0 33.0 37.0 7 35.565553012887996 37.0 35.0 37.0 33.0 37.0 8 35.56772329431067 37.0 35.0 37.0 33.0 37.0 9 37.15498950554707 39.0 37.0 39.0 33.0 39.0 10-11 37.05610540921876 39.0 37.0 39.0 33.0 39.0 12-13 36.972101110192405 39.0 37.0 39.0 33.0 39.0 14-15 38.01361098743911 40.0 37.0 41.0 33.0 41.0 16-17 37.93300034916609 40.0 37.0 41.0 33.0 41.0 18-19 38.08704944269717 40.0 37.0 41.0 33.0 41.0 20-21 38.20523632313829 40.0 37.0 41.0 33.0 41.0 22-23 38.119787333188825 40.0 37.0 41.0 33.0 41.0 24-25 38.01754916557093 40.0 37.0 41.0 33.0 41.0 26-27 37.80263419205693 40.0 37.0 41.0 33.0 41.0 28-29 37.73287431188969 40.0 37.0 41.0 32.5 41.0 30-31 37.67458823712661 39.0 37.0 41.0 33.0 41.0 32-33 37.45802802934033 39.0 36.0 41.0 32.5 41.0 34-35 37.28301728826452 39.0 36.0 41.0 32.0 41.0 36-37 37.03050335731633 39.0 35.5 41.0 31.0 41.0 38-39 36.880454746646905 39.0 35.0 41.0 31.0 41.0 40-41 36.68126457833327 39.0 35.0 41.0 30.5 41.0 42-43 36.41048173238301 39.0 35.0 40.5 30.0 41.0 44-45 36.084768829982075 39.0 35.0 40.0 30.0 41.0 46-47 35.59995560787999 39.0 35.0 40.0 28.0 41.0 48-49 35.08751802618616 38.5 35.0 40.0 25.5 41.0 50-51 34.91327376308886 38.5 35.0 40.0 24.5 41.0 52-53 34.70204515275692 38.0 34.0 40.0 24.0 41.0 54-55 34.48692184780252 38.0 34.0 40.0 23.5 41.0 56-57 34.26206407781861 38.0 34.0 40.0 23.0 41.0 58-59 33.974638729918084 38.0 33.5 40.0 22.5 41.0 60-61 33.63558288021786 38.0 33.0 40.0 21.0 41.0 62-63 33.38911575310193 37.5 33.0 40.0 20.5 41.0 64-65 32.984536744863426 37.0 32.0 39.5 18.5 40.5 66-67 32.603985296083756 36.5 32.0 39.0 18.0 40.0 68-69 32.137187876018785 36.0 31.0 39.0 13.5 40.0 70-71 31.663135848973333 35.5 31.0 38.0 11.5 40.0 72-73 31.249984748321342 35.0 31.0 38.0 8.0 39.5 74-75 30.855316086172635 35.0 30.0 37.0 7.5 39.0 76-77 29.342208728633047 33.0 28.5 35.5 6.5 38.0 78-79 30.117912350225268 34.0 30.0 36.0 2.0 39.0 80-81 29.90806807310941 34.0 29.5 36.0 2.0 37.5 82-83 29.529917952458895 34.0 29.0 35.5 2.0 37.0 84-85 29.049604949072375 34.0 29.0 35.0 2.0 37.0 86-87 28.68951346496073 34.0 29.0 35.0 2.0 36.0 88-89 28.2484264810678 34.0 28.5 35.0 2.0 36.0 90-91 27.78823725641122 33.0 27.0 35.0 2.0 35.5 92-93 27.362200109812086 33.0 26.0 35.0 2.0 35.0 94-95 26.8398878774466 33.0 25.5 34.5 2.0 35.0 96-97 26.16939552729921 32.0 25.0 34.0 2.0 35.0 98-99 25.392475665459948 32.0 22.0 34.0 2.0 35.0 100-101 22.609298721325224 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.33412084602218073 1101 2 0.2852279737102634 1101 3 0.1353583118716415 1101 4 0.10205423946781877 1101 5 0.14344496609290758 1101 6 0.1685729319728395 1101 7 0.09452627864882146 1101 8 0.2171130173885203 1101 9 0.2856514970845865 1101 10-11 0.20797912231834204 1101 12-13 0.12084873900452209 1101 14-15 0.21852627634854116 1101 16-17 0.05564593001469831 1101 18-19 0.16799647211919222 1101 20-21 0.08473802338785674 1101 22-23 0.03205525389653019 1101 24-25 0.1749680392402979 1101 26-27 0.16882748096049482 1101 28-29 0.09997339519281212 1101 30-31 -0.06581978579933434 1101 32-33 0.11576977575268899 1101 34-35 0.3865838657345293 1101 36-37 0.33211469928628645 1101 38-39 0.2986050680922645 1101 40-41 0.41119118601346116 1101 42-43 0.43502895503799976 1101 44-45 0.5230549066395085 1101 46-47 0.4759225033084604 1101 48-49 0.2973174745846521 1101 50-51 0.25742809956962276 1101 52-53 0.17740450717670342 1101 54-55 0.12451886612615937 1101 56-57 0.10422666161114336 1101 58-59 0.17709614794748774 1101 60-61 0.19930826421647652 1101 62-63 0.15467792570619565 1101 64-65 0.13527422079503282 1101 66-67 -0.2192145758609101 1101 68-69 -0.11121546878311506 1101 70-71 -0.2215545116193809 1101 72-73 0.05050652707719294 1101 74-75 0.30984046091315776 1101 76-77 0.505912897571875 1101 78-79 0.1976255095651389 1101 80-81 0.1488140810113059 1101 82-83 0.29648286860226136 1101 84-85 0.3513871581553296 1101 86-87 0.4577220811598899 1101 88-89 0.5470375879392932 1101 90-91 0.3611231310559866 1101 92-93 0.46019435718414314 1101 94-95 0.23587178348916993 1101 96-97 0.4752951269255128 1101 98-99 0.47596163970667504 1101 100-101 0.7083760332593272 1102 1 0.5617843447045701 1102 2 0.5686432027093495 1102 3 0.3189640623576224 1102 4 0.21258711894154914 1102 5 0.26774571905372824 1102 6 0.2865607596131099 1102 7 0.3349829218759055 1102 8 0.03396352300323002 1102 9 -0.05746000361085635 1102 10-11 0.08449479125892623 1102 12-13 0.33833910923561206 1102 14-15 0.38716544413740905 1102 16-17 0.4833397075110426 1102 18-19 0.35826079648685294 1102 20-21 0.37392558335788095 1102 22-23 0.12173169919142879 1102 24-25 0.1949962994666663 1102 26-27 0.1938095763650196 1102 28-29 0.1779282064825196 1102 30-31 0.21639880788109878 1102 32-33 0.46960052962602816 1102 34-35 0.5529472833968967 1102 36-37 0.46916504507310464 1102 38-39 0.3510523981645193 1102 40-41 0.5469094022456673 1102 42-43 0.5283471535730015 1102 44-45 0.4998853922895563 1102 46-47 0.644795329524662 1102 48-49 0.6316847579805298 1102 50-51 0.6442675997341496 1102 52-53 0.5016650266450071 1102 54-55 0.6967739326427775 1102 56-57 0.7424176476497095 1102 58-59 0.745524193595223 1102 60-61 0.7254413693295945 1102 62-63 0.7012976210377246 1102 64-65 0.826413036022366 1102 66-67 0.8530204430391493 1102 68-69 0.9742737898422078 1102 70-71 0.5811112683435127 1102 72-73 0.7960981253284949 1102 74-75 0.31118918159870645 1102 76-77 0.7249586863280655 1102 78-79 1.185882445349165 1102 80-81 1.0702647541964438 1102 82-83 1.1807313871525267 1102 84-85 1.2891047439245646 1102 86-87 1.1278952372630755 1102 88-89 1.1332001699616967 1102 90-91 1.180501018949716 1102 92-93 1.3394924970418458 1102 94-95 1.1215934562069911 1102 96-97 0.9224883223496789 1102 98-99 0.7780391258185162 1102 100-101 1.1578401397301477 1103 1 0.45883092830425554 1103 2 0.343647967611151 1103 3 0.19646974938790152 1103 4 0.14706457718524035 1103 5 0.1191548273520624 1103 6 0.06654707508937463 1103 7 0.17142406813987776 1103 8 0.2718987230811152 1103 9 0.14880333982818428 1103 10-11 0.19810513335830393 1103 12-13 0.1536048947725277 1103 14-15 0.29837989762717143 1103 16-17 0.24228229695750514 1103 18-19 0.410777555914855 1103 20-21 0.21124566546502166 1103 22-23 0.1807924856264691 1103 24-25 0.13720249699275655 1103 26-27 0.26126961632163415 1103 28-29 0.30552610636063093 1103 30-31 0.19088224453395952 1103 32-33 0.011708549607071461 1103 34-35 0.2658773757742878 1103 36-37 0.3111615869199156 1103 38-39 0.4820988528718999 1103 40-41 0.6334647004736738 1103 42-43 0.3430260677512891 1103 44-45 0.3416776261166632 1103 46-47 0.4885278039416008 1103 48-49 0.5340700883904717 1103 50-51 0.32159119209988773 1103 52-53 0.5883917242184111 1103 54-55 0.42074762764243445 1103 56-57 0.6380340751114559 1103 58-59 0.5365472557199453 1103 60-61 0.4953062106617878 1103 62-63 0.2900152934071869 1103 64-65 0.5181341734010019 1103 66-67 0.7065007410271988 1103 68-69 0.5170009042588006 1103 70-71 0.47573152280983777 1103 72-73 0.5411004632155603 1103 74-75 0.48666508902112326 1103 76-77 0.5882327429117886 1103 78-79 0.6499146355174226 1103 80-81 0.6422099722232666 1103 82-83 0.8428340320501064 1103 84-85 0.936088595363838 1103 86-87 0.8530488453564153 1103 88-89 0.6059716699527833 1103 90-91 0.6691269583895263 1103 92-93 0.686635132834688 1103 94-95 0.7210338716241544 1103 96-97 0.6675263113005911 1103 98-99 0.6145947668452081 1103 100-101 0.5386003575307257 1104 1 0.1996343428087961 1104 2 0.18979776990463293 1104 3 0.014991788372245907 1104 4 -0.04059251176209244 1104 5 -0.12364270213341655 1104 6 0.07742342006913816 1104 7 -0.1313432548016067 1104 8 0.026751164255792048 1104 9 -0.059447053382591264 1104 10-11 -0.12254315647675895 1104 12-13 -0.02537385124387015 1104 14-15 -0.018910294055089594 1104 16-17 -0.10347962262148513 1104 18-19 -0.18485116218250397 1104 20-21 -0.32070137788832653 1104 22-23 -0.24385596902321538 1104 24-25 -0.16350890548032027 1104 26-27 -0.031376525239927844 1104 28-29 -0.04282951246859312 1104 30-31 -0.1527093789043832 1104 32-33 -0.019242501867658746 1104 34-35 0.04852381570717057 1104 36-37 0.11098071706806678 1104 38-39 0.05645085430408159 1104 40-41 -0.10995869837347527 1104 42-43 -0.021728607087908358 1104 44-45 0.19957757510937313 1104 46-47 0.04345228047753125 1104 48-49 0.48255983223159404 1104 50-51 0.29100244865517055 1104 52-53 0.26570953655990337 1104 54-55 0.4687413646863732 1104 56-57 0.39155521187776543 1104 58-59 0.4243369067817895 1104 60-61 0.29461597834418995 1104 62-63 -0.04180984395017617 1104 64-65 0.2688904026126906 1104 66-67 0.4173320461459653 1104 68-69 0.3600710882970759 1104 70-71 0.1318926774215896 1104 72-73 0.07477237695499284 1104 74-75 0.05839923733439889 1104 76-77 0.4056088939501912 1104 78-79 0.47404889692763774 1104 80-81 0.351018035637118 1104 82-83 0.3916788377606508 1104 84-85 0.23707563610289029 1104 86-87 0.11639492340750834 1104 88-89 0.21178763736495299 1104 90-91 0.06714790405611026 1104 92-93 -0.05354454353188487 1104 94-95 0.13036877353813736 1104 96-97 -0.07737686720644277 1104 98-99 0.3325713546423934 1104 100-101 0.23358956643469142 1105 1 0.12617005902959733 1105 2 0.2974526105369115 1105 3 0.09981870393601255 1105 4 -0.024785492567239942 1105 5 -0.13036275277975307 1105 6 -0.17919468200491195 1105 7 0.06533764188955615 1105 8 -0.08919359245071234 1105 9 -0.01791762517862594 1105 10-11 -0.029699375910006154 1105 12-13 -0.0279624894675905 1105 14-15 0.041252196284752074 1105 16-17 -0.08031876060709209 1105 18-19 -0.1541945848598587 1105 20-21 -0.011239694867114736 1105 22-23 0.04186809397671709 1105 24-25 -0.12455100525018281 1105 26-27 -0.1130986433747907 1105 28-29 -0.02995618820369117 1105 30-31 -0.0659447275399927 1105 32-33 0.03777384127269556 1105 34-35 -0.18829273674857916 1105 36-37 0.10564445788200061 1105 38-39 0.024144563720703616 1105 40-41 -0.16262832511337422 1105 42-43 0.0686085218691943 1105 44-45 -0.00834618161709244 1105 46-47 -0.04825723310657537 1105 48-49 -0.004352252452740402 1105 50-51 -0.16854321717594445 1105 52-53 -0.20114790939309302 1105 54-55 0.11024883473630354 1105 56-57 0.105883192554586 1105 58-59 0.060361254667391506 1105 60-61 0.04736787686901067 1105 62-63 -0.029532526723293984 1105 64-65 -0.20461223664335648 1105 66-67 -0.04752777813175868 1105 68-69 -0.1506577513142915 1105 70-71 -0.2995715036961073 1105 72-73 -0.35306371825784666 1105 74-75 -0.22891394012455635 1105 76-77 -0.23816711006501023 1105 78-79 -0.11671527534976178 1105 80-81 -0.5365840198742369 1105 82-83 -0.194076308859934 1105 84-85 -0.09455237565755326 1105 86-87 -0.17086121988512915 1105 88-89 -0.22885904452983397 1105 90-91 -0.1806733907905702 1105 92-93 -0.041073310139523755 1105 94-95 0.01822748279792208 1105 96-97 -0.07970029655679767 1105 98-99 -0.17165772879003782 1105 100-101 0.012739728839481046 1106 1 0.3913122133201803 1106 2 0.35045226636498583 1106 3 0.11395796379710177 1106 4 0.018887668228757093 1106 5 -0.14099258740796472 1106 6 0.05546004584537201 1106 7 0.07268091671365795 1106 8 0.00605739915938841 1106 9 0.03961268734077095 1106 10-11 -0.019257143193364357 1106 12-13 0.041963654621966384 1106 14-15 -0.023151681009494496 1106 16-17 0.06283701386438167 1106 18-19 -0.16186792620506907 1106 20-21 -0.12564723956715085 1106 22-23 -0.10135961806986415 1106 24-25 -0.05904972730031943 1106 26-27 0.04413714824885062 1106 28-29 -0.03615974615152595 1106 30-31 -0.04839054867213832 1106 32-33 0.05727879791268009 1106 34-35 -0.40715646159158325 1106 36-37 -0.2745752479356369 1106 38-39 -0.09137871866389702 1106 40-41 -0.14853279201362568 1106 42-43 -0.31140101338586135 1106 44-45 -0.21016070115872054 1106 46-47 -0.23236382484532925 1106 48-49 -0.3760565241341638 1106 50-51 -0.30239083364428865 1106 52-53 -0.08183732525998977 1106 54-55 -0.37718465827023095 1106 56-57 -0.3276366142577629 1106 58-59 -0.464601083896234 1106 60-61 0.18563471731427938 1106 62-63 -0.38429338365497756 1106 64-65 -0.21729863959533446 1106 66-67 -0.1687933716590706 1106 68-69 -0.09705297353087161 1106 70-71 0.04336479064424026 1106 72-73 0.0724879442054096 1106 74-75 0.07330850778417641 1106 76-77 0.2905091821313128 1106 78-79 0.3377595239302984 1106 80-81 0.23932338095064765 1106 82-83 0.2618922530092007 1106 84-85 0.46924496914065017 1106 86-87 0.43180498412419155 1106 88-89 -0.08672309097612185 1106 90-91 -0.019115702497490616 1106 92-93 0.1858178017614911 1106 94-95 0.26167975292210244 1106 96-97 0.31780840647925146 1106 98-99 0.3105511215064567 1106 100-101 0.21614251719631383 1107 1 0.5558533009100195 1107 2 0.5503709114855475 1107 3 0.3294808798800517 1107 4 0.18634200316105165 1107 5 0.05736985026778285 1107 6 0.020602349369958972 1107 7 0.11702776530919579 1107 8 0.2352452344106979 1107 9 0.388066438273313 1107 10-11 0.33855241887433607 1107 12-13 0.2499966894290253 1107 14-15 0.513955089674397 1107 16-17 0.4769780784993145 1107 18-19 0.46748042458637684 1107 20-21 0.45518187130166154 1107 22-23 0.481233307093774 1107 24-25 0.32934218042096575 1107 26-27 0.28384963807949504 1107 28-29 0.46056664827448657 1107 30-31 0.5373519243136471 1107 32-33 0.4239082753847967 1107 34-35 0.511816103922726 1107 36-37 0.5736869308222978 1107 38-39 0.5933837824754065 1107 40-41 0.30897278636555114 1107 42-43 0.5472558546875845 1107 44-45 0.24028202466858772 1107 46-47 0.38312465888201075 1107 48-49 0.21146157817044298 1107 50-51 0.24670818638386294 1107 52-53 0.05469287225345454 1107 54-55 0.36400977314017524 1107 56-57 0.26328168316351963 1107 58-59 0.008882626561415918 1107 60-61 0.03668861745369867 1107 62-63 0.07128887760558911 1107 64-65 0.04808620949305009 1107 66-67 0.2916237662020009 1107 68-69 0.3066427659714819 1107 70-71 0.2686578876320844 1107 72-73 0.22298096827767822 1107 74-75 0.06802297241168276 1107 76-77 0.4031468693572471 1107 78-79 0.5340332341516465 1107 80-81 0.5394849092071219 1107 82-83 0.36659024411755325 1107 84-85 0.5134289391912539 1107 86-87 0.9660314207467131 1107 88-89 0.8398786043110356 1107 90-91 0.575437402659599 1107 92-93 0.9885820110156942 1107 94-95 1.0656651292981323 1107 96-97 1.2046187888471849 1107 98-99 1.4280861361739667 1107 100-101 1.3199822312052198 1108 1 0.35090812601454147 1108 2 0.2656802967876146 1108 3 0.13688946954390957 1108 4 -0.035833132640462395 1108 5 -0.09795112930098782 1108 6 -0.005224246630383789 1108 7 -0.017262095027589908 1108 8 0.1271690571431634 1108 9 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-1.2692251452701804 2316 78-79 -1.131115428228096 2316 80-81 -1.39225192251293 2316 82-83 -0.955316411066498 2316 84-85 -1.312770788250294 2316 86-87 -1.1300841602656604 2316 88-89 -1.4322188233494053 2316 90-91 -1.2612727909896826 2316 92-93 -1.5861115451344823 2316 94-95 -1.6581859483035757 2316 96-97 -1.9200896123337792 2316 98-99 -1.9544656969568237 2316 100-101 -2.818637162490532 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 3.0 5 22.0 6 71.0 7 149.0 8 331.0 9 624.0 10 1158.0 11 1802.0 12 2599.0 13 3496.0 14 4552.0 15 6510.0 16 8759.0 17 11415.0 18 9344.0 19 5484.0 20 4593.0 21 4796.0 22 4989.0 23 5357.0 24 6483.0 25 7751.0 26 9150.0 27 11486.0 28 14221.0 29 17790.0 30 21912.0 31 27211.0 32 33366.0 33 41522.0 34 53828.0 35 71955.0 36 100969.0 37 151020.0 38 117713.0 39 7973.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.11750397003883 24.208778801631816 16.52781912096587 20.14589810736349 2 14.379424360873363 24.102535476023256 40.13155759571808 21.386482567385297 3 18.026379497670604 28.722394794196564 32.94581441928629 20.305411288846543 4 11.694290139283577 20.69754731604221 41.064054347727314 26.544108196946897 5 10.518725816354213 40.62982293774589 36.82222513554524 12.029226110354656 6 25.634632084080234 42.04933236587933 18.419225162803556 13.896810387236878 7 23.697474192212688 36.261871971568276 24.040539610881 16.000114225338034 8 23.56754287019718 35.82002759580325 21.623505497743402 18.98892403625616 9 24.340027493175384 18.82516651024893 24.3293832987397 32.50542269783598 10-11 22.13116616011459 29.115484138527226 29.36450916369258 19.3888405376656 12-13 24.299523366960713 27.937030441171125 29.134324328534124 18.629121863334042 14-15 21.75382622432039 29.433143779846237 28.644275039037808 20.168754956795564 16-17 18.12808035233325 32.12717433772019 30.300412639033652 19.444332670912907 18-19 17.53612051811575 30.93747850162317 33.389494124534174 18.13690685572691 20-21 18.673116936891798 29.362531752696952 33.334977485926274 18.62937382448498 22-23 18.671477173457323 29.352204123328622 33.024271901370334 18.952046801843725 24-25 19.135989158977008 30.14588392888434 32.99308028094241 17.725046631196236 26-27 18.698947439470306 29.171983120610275 34.37403865748883 17.755030782430588 28-29 17.59076695824415 29.773288664303415 34.4634719051099 18.17247247234254 30-31 18.579530040614895 29.3742138895415 33.642801791780194 18.403454278063414 32-33 18.406538131528492 29.790828598613917 33.69582007676801 18.106813193089582 34-35 19.25188456683357 29.46252121455992 33.28837847748417 17.997215741122336 36-37 19.19841273962076 28.94829072254875 33.44299725851004 18.410299279320444 38-39 18.23803407681529 30.38882719707064 33.4725888216156 17.90054990449847 40-41 18.276022537061472 29.873483775120057 34.061571272710516 17.788922415107958 42-43 18.174483610478443 30.032668050931782 32.824348699801185 18.96849963878859 44-45 18.790924862131607 29.675469777989218 32.47074048810311 19.062864871776068 46-47 19.81366641030384 30.4398731815276 31.45902900145056 18.287431406718007 48-49 19.133296336322303 30.339755884634233 31.524958378362587 19.001989400680873 50-51 18.572069239221367 30.511363230730986 31.881298258116058 19.035269271931586 52-53 19.010281791973167 31.5327541469202 31.067730542023465 18.389233519083167 54-55 18.975432447343653 30.588388018044334 31.02047836951936 19.415701165092656 56-57 19.4095606623004 31.04674278971748 30.32105771795702 19.222638830025097 58-59 19.58844649129411 30.24780697769205 31.024094039704153 19.139652491309686 60-61 19.27562344309601 30.83698639205877 31.46026722605954 18.427122938785683 62-63 18.985704401870773 30.404009984721764 31.769510252772342 18.840775360635117 64-65 18.95768529721814 31.0286183134357 30.90859639921275 19.105099990133407 66-67 18.951466871586916 30.77989981580543 30.177924948824025 20.090708363783634 68-69 18.70274809824177 30.507765066578575 30.95259574341045 19.836891091769203 70-71 19.499244869362727 30.56529981639325 30.84528551207073 19.090169802173296 72-73 19.157730913659922 29.524783653315716 31.68000809961488 19.637477333409485 74-75 18.655204327063487 31.3697824656318 30.324425920325233 19.65058728697948 76-77 18.777782032452205 30.66610678035734 30.824597008415815 19.73151417877464 78-79 18.948808003889265 30.341070106078533 30.83592585212355 19.87419603790865 80-81 19.04565317297731 30.641670756056843 30.231628252363407 20.08104781860244 82-83 19.03567582724651 30.854855470806292 30.517654221701218 19.59181448024598 84-85 18.573527827286878 30.56815098767948 30.26756981648387 20.59075136854977 86-87 18.205149508130326 30.782032542066606 30.61781635307649 20.39500159672658 88-89 19.062970866260837 30.412060927862562 30.0265713827571 20.498396823119506 90-91 18.8139874412424 29.708154623454742 30.780965685755813 20.696892249547044 92-93 19.23823802384522 30.330029790299655 30.598531902879728 19.833200282975397 94-95 18.95955032842641 29.559415323104083 30.681776877742294 20.799257470727213 96-97 18.851028917702163 29.677707042750846 30.39720948587867 21.074054553668326 98-99 18.744194831672026 30.23386836620576 29.56590484684092 21.4560319552813 100-101 18.215765399412057 30.063668882863926 30.01219633305141 21.708369384672604 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 774.0 1 509.0 2 262.0 3 291.0 4 288.5 5 304.0 6 437.0 7 751.5 8 1105.0 9 1332.0 10 1281.0 11 1121.5 12 1109.5 13 1128.5 14 1165.0 15 1215.0 16 1335.0 17 1446.5 18 1557.0 19 1750.5 20 1968.5 21 2251.0 22 2532.5 23 3010.0 24 3478.0 25 4447.5 26 6670.0 27 9264.5 28 10447.5 29 11804.0 30 14567.5 31 17427.5 32 21806.5 33 27461.0 34 33183.5 35 37645.5 36 44019.5 37 49564.5 38 47577.5 39 43461.0 40 39202.0 41 34030.0 42 29822.0 43 27272.0 44 26135.0 45 25919.5 46 23986.5 47 24489.5 48 33769.5 49 30618.5 50 16701.0 51 10306.5 52 7219.5 53 5770.5 54 4771.0 55 4011.5 56 3289.5 57 2676.0 58 2224.0 59 1871.0 60 1493.5 61 1117.0 62 807.5 63 505.5 64 285.0 65 183.0 66 130.0 67 102.0 68 68.5 69 41.0 70 38.0 71 35.5 72 27.5 73 23.5 74 21.5 75 22.0 76 15.5 77 8.5 78 7.5 79 7.0 80 7.0 81 5.5 82 3.0 83 2.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0150478902709865 2 0.012720549008511087 3 0.004672854737820398 4 0.014018564213461197 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004413251696830376 10-11 3.245038012375277E-4 12-13 3.894045614850332E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0012980152049501107 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.004413251696830376 34-35 5.841068422275498E-4 36-37 0.0029854349713852547 38-39 6.490076024750554E-5 40-41 0.004088747895592849 42-43 0.010708625440838414 44-45 0.002141725088167683 46-47 0.0018821220471776606 48-49 0.009670213276878326 50-51 7.139083627225609E-4 52-53 0.0020768243279201772 54-55 0.010643724680590909 56-57 0.004088747895592849 58-59 0.0011682136844550996 60-61 0.006230472983760531 62-63 0.0038940456148503326 64-65 0.01700399918484645 66-67 0.003374839532870288 68-69 1.947022807425166E-4 70-71 0.0013629159651976164 72-73 0.0 74-75 0.0 76-77 0.0016225190061876385 78-79 0.010578823920343403 80-81 0.01570598397989634 82-83 0.012396045207273557 84-85 0.008566900352670732 86-87 0.010513923160095898 88-89 0.02823183070766491 90-91 0.024337785092814576 92-93 0.0028556334508902438 94-95 0.008696701873165742 96-97 0.01077352620108592 98-99 0.010449022399848391 100-101 0.012136442166283536 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 770407.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.936313897423904 #Duplication Level Percentage of deduplicated Percentage of total 1 74.19944915303589 22.212580008612267 2 12.496599443951025 7.482042472089819 3 4.474004707932148 4.018056279456275 4 2.1193897475656938 2.537868670164345 5 1.2802474090949194 1.9162944152514592 6 0.7937961279606772 1.4257998034314303 7 0.5483787735501634 1.1491507369777423 8 0.42085923731773084 1.007917938797921 9 0.3430350769571376 0.9242285167454253 >10 2.472266779749993 15.600098323951778 >50 0.44109889856461665 9.319468445248374 >100 0.3784602267510267 22.017653984831124 >500 0.023215731502088444 4.522309107327142 >1k 0.008322620727163782 4.48192081014105 >5k 8.760653397014508E-4 1.3846104869738536 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5429 0.7046924547674152 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5130 0.6658818001394068 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4942 0.6414791142863447 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2902 0.37668401247652217 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2583 0.3352773274386136 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2373 0.3080190081346613 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1825 0.23688777490339522 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1730 0.22455663045636917 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1724 0.22377782133339907 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1588 0.20612481454607762 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1578 0.2048267993411275 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1566 0.20326918109518735 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1546 0.20067315068528713 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1475 0.19145724273014134 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1449 0.18808240319727107 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1358 0.17627046483222503 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1244 0.16147309149579378 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1159 0.15043996225371786 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1078 0.13992603909362195 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1046 0.13577239043778158 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1013 0.13148894026144622 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 858 0.11136970458471951 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 847 0.10994188785927439 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 843 0.10942268177729433 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 841 0.10916307873630432 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 839 0.10890347569531429 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 835 0.10838426961333426 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 806 0.10462002551897893 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 795 0.1031922087935338 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 788 0.10228359815006872 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.490076024750554E-5 0.0 14-15 0.0 0.0 0.0 1.2980152049501108E-4 0.0 16-17 0.0 0.0 0.0 1.2980152049501108E-4 0.0 18-19 0.0 0.0 0.0 1.2980152049501108E-4 0.0 20-21 0.0 0.0 0.0 1.9470228074251663E-4 0.0 22-23 0.0 0.0 0.0 3.8940456148503326E-4 0.0 24-25 0.0 0.0 0.0 0.0013629159651976164 0.0 26-27 0.0 0.0 0.0 0.0023364273689101996 0.0 28-29 0.0 0.0 0.0 0.005776167662027993 0.0 30-31 0.0 0.0 0.0 0.013174854330243624 0.0 32-33 0.0 0.0 0.0 0.02394838053132954 0.0 34-35 0.0 0.0 0.0 0.038161647025533256 0.0 36-37 0.0 0.0 0.0 0.05218021123899445 0.0 38-39 0.0 0.0 0.0 0.06288883667983286 0.0 40-41 0.0 0.0 0.0 0.07249414919646369 0.0 42-43 0.0 0.0 0.0 0.08300807235655958 0.0 44-45 0.0 0.0 0.0 0.09352199551665548 0.0 46-47 0.0 0.0 0.0 0.11299222359090715 0.0 48-49 0.0 0.0 0.0 0.13518828359555404 0.0 50-51 0.0 0.0 0.0 0.15472341243005322 0.0 52-53 0.0 0.0 0.0 0.1850320674656383 0.0 54-55 0.0 0.0 0.0 0.2472069957827486 0.0 56-57 0.0 0.0 0.0 0.3550720593141028 0.0 58-59 0.0 0.0 0.0 0.46566295477585223 0.0 60-61 0.0 0.0 0.0 0.5682710567271585 0.0 62-63 0.0 0.0 0.0 0.6869745472198461 0.0 64-65 0.0 0.0 0.0 0.8399456391232167 0.0 66-67 0.0 0.0 0.0 1.0740426813359691 0.0 68-69 0.0 0.0 0.0 1.3504550192300953 0.0 70-71 0.0 0.0 0.0 1.5852659698055702 0.0 72-73 0.0 0.0 0.0 1.8157285694444625 0.0 74-75 0.0 0.0 0.0 2.0840283123076504 0.0 76-77 0.0 0.0 0.0 2.386011549739294 0.0 78-79 0.0 0.0 0.0 2.6386702093828327 0.0 80-81 0.0 0.0 0.0 2.8965209298461723 0.0 82-83 0.0 0.0 0.0 3.165923985633568 0.0 84-85 0.0 0.0 0.0 3.595956423033539 0.0 86-87 0.0 0.0 0.0 4.159295021981888 0.0 88-89 0.0 0.0 0.0 4.796815189893135 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 240 0.0 39.577625 9 GGCATTC 290 0.0 32.7539 7 TTAGGCA 325 0.0 30.689877 4 CCTTTAG 360 0.0 30.65722 1 GGACGAG 65 0.0058470103 29.226553 7 CTTTAGG 375 0.0 26.601347 2 GGGGAGC 190 1.19162E-8 24.996397 7 GGATGAC 330 0.0 24.466171 8 GTACATG 3575 0.0 24.026163 1 GACTGGT 100 0.00165454 23.746578 7 GAGTACT 4635 0.0 23.618494 12-13 GACATAT 410 0.0 23.407423 1 ACATGGG 3575 0.0 22.985685 3 TAGGCAT 420 0.0 22.615788 5 GTACTTT 4915 0.0 22.22467 14-15 CATATAG 460 0.0 21.684471 3 ATGGGGA 570 0.0 21.663544 5 TAGACCC 110 0.002877837 21.589197 4 ACACGGA 400 0.0 21.37192 6 CACGGAG 445 0.0 21.345238 7 >>END_MODULE