##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936645_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 787067 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.764685852665657 31.0 31.0 34.0 30.0 34.0 2 31.90998352109795 31.0 31.0 34.0 30.0 34.0 3 31.99387472731038 31.0 31.0 34.0 30.0 34.0 4 35.76638837608488 37.0 35.0 37.0 35.0 37.0 5 35.53471813708363 37.0 35.0 37.0 33.0 37.0 6 35.65453512852146 37.0 35.0 37.0 33.0 37.0 7 35.601950024584944 37.0 35.0 37.0 33.0 37.0 8 35.60029578168059 37.0 35.0 37.0 33.0 37.0 9 37.18701838598239 39.0 37.0 39.0 33.0 39.0 10-11 37.06465142103531 39.0 37.0 39.0 33.0 39.0 12-13 37.00511646403673 39.0 37.0 39.0 33.0 39.0 14-15 38.009752664004466 40.0 37.0 41.0 33.0 41.0 16-17 37.952100647085956 40.0 37.0 41.0 33.0 41.0 18-19 38.18421366923019 40.0 37.5 41.0 33.0 41.0 20-21 38.264737944800125 40.0 38.0 41.0 33.0 41.0 22-23 38.165560873470746 40.0 37.0 41.0 33.0 41.0 24-25 38.05523100320557 40.0 37.0 41.0 33.0 41.0 26-27 37.81890931775821 40.0 37.0 41.0 33.0 41.0 28-29 37.75899192826024 40.0 37.0 41.0 32.5 41.0 30-31 37.67756302830635 39.5 37.0 41.0 33.0 41.0 32-33 37.480413357439716 39.0 36.0 41.0 32.0 41.0 34-35 37.29612663216727 39.0 36.0 41.0 32.0 41.0 36-37 37.02225287046719 39.0 35.5 41.0 31.0 41.0 38-39 36.85597604778246 39.0 35.0 41.0 31.0 41.0 40-41 36.66709568562778 39.0 35.0 41.0 30.5 41.0 42-43 36.40650478294732 39.0 35.0 40.5 30.0 41.0 44-45 36.09720455818882 39.0 35.0 40.0 30.0 41.0 46-47 35.65637868186571 39.0 35.0 40.0 28.5 41.0 48-49 35.22400253091541 39.0 35.0 40.0 26.5 41.0 50-51 34.93036234526413 38.5 35.0 40.0 25.0 41.0 52-53 34.7145300209512 38.0 34.0 40.0 24.0 41.0 54-55 34.498449306094656 38.0 34.0 40.0 23.5 41.0 56-57 34.223537513324786 38.0 34.0 40.0 23.0 41.0 58-59 33.958607081735096 38.0 33.0 40.0 22.0 41.0 60-61 33.64225980761485 38.0 33.0 40.0 21.5 41.0 62-63 33.296110115149034 37.0 32.5 40.0 20.0 41.0 64-65 32.915973481291935 37.0 32.0 39.0 18.5 40.5 66-67 32.47369855425269 36.0 31.5 39.0 16.0 40.0 68-69 32.18118978435127 36.0 31.0 39.0 14.0 40.0 70-71 31.760486083141586 35.5 31.0 38.5 10.5 40.0 72-73 31.291169620883608 35.0 30.5 38.0 8.0 39.5 74-75 30.73362178823404 35.0 30.0 37.0 7.0 39.0 76-77 29.226690358000017 33.0 28.5 35.5 4.0 38.0 78-79 29.98334957506794 34.0 29.0 36.0 2.0 38.5 80-81 29.73630262734939 34.0 29.0 36.0 2.0 37.5 82-83 29.370193388872863 34.0 29.0 35.5 2.0 37.0 84-85 28.912134545089554 34.0 29.0 35.0 2.0 36.5 86-87 28.556839506674784 34.0 29.0 35.0 2.0 36.0 88-89 28.12133465130669 33.5 28.5 35.0 2.0 36.0 90-91 27.63717193072508 33.0 26.5 35.0 2.0 35.0 92-93 27.16077093817934 33.0 26.0 35.0 2.0 35.0 94-95 26.584626213524388 32.5 25.5 34.0 2.0 35.0 96-97 25.826285436945014 32.0 24.5 34.0 2.0 35.0 98-99 24.963840435439423 32.0 19.0 34.0 2.0 35.0 100-101 21.76890976244716 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2544522322669316 1101 2 0.41162608033506487 1101 3 0.325210627340649 1101 4 0.13989850273605953 1101 5 0.14216431075086433 1101 6 0.2325928911697588 1101 7 0.12345635188441406 1101 8 0.27378499663061007 1101 9 0.4808924302600559 1101 10-11 0.45866786170557106 1101 12-13 0.3978029028282819 1101 14-15 0.4397551454705422 1101 16-17 0.45393276228683277 1101 18-19 0.3384634931038448 1101 20-21 0.33311583692306357 1101 22-23 0.24940579406879948 1101 24-25 0.2591658369821985 1101 26-27 0.19906427631610768 1101 28-29 0.2494685660173772 1101 30-31 0.4594867680547452 1101 32-33 0.3894945845529989 1101 34-35 0.22981423933919132 1101 36-37 0.17824372425027235 1101 38-39 0.24723761613043393 1101 40-41 0.3139091490406898 1101 42-43 0.4233277240333635 1101 44-45 0.3243574007999257 1101 46-47 0.3620413087895784 1101 48-49 0.6962387129059167 1101 50-51 0.6841786714114946 1101 52-53 0.8076341815110766 1101 54-55 0.7019145410864169 1101 56-57 0.5179776361586761 1101 58-59 0.6531318932244048 1101 60-61 0.49918717955042524 1101 62-63 0.613335149836999 1101 64-65 0.6074995886674657 1101 66-67 0.36929717347249635 1101 68-69 0.30877361794602365 1101 70-71 0.5033183653740068 1101 72-73 0.6116349168006572 1101 74-75 0.2686246280103788 1101 76-77 0.3081508693017234 1101 78-79 0.2799112965808952 1101 80-81 0.3975303495724205 1101 82-83 0.5844440701931184 1101 84-85 0.5959745227447115 1101 86-87 0.7055147705701152 1101 88-89 0.6945801754860383 1101 90-91 0.5353798953416025 1101 92-93 0.5614514326449545 1101 94-95 0.6365484893795745 1101 96-97 0.40227264383355177 1101 98-99 0.5163300809266467 1101 100-101 1.0159921976592727 1102 1 0.13382665248124326 1102 2 0.32656308293996616 1102 3 0.21156832387901758 1102 4 0.20046905493615697 1102 5 0.08532305499608839 1102 6 0.021107175078782348 1102 7 0.1962243026238113 1102 8 0.159189188032407 1102 9 0.13049508858642156 1102 10-11 0.13693814020373907 1102 12-13 0.1982051269077374 1102 14-15 0.2529370667833888 1102 16-17 0.2275406304274128 1102 18-19 0.28999340932750783 1102 20-21 0.06934099050344855 1102 22-23 0.17329124134025164 1102 24-25 0.07402500829432057 1102 26-27 -0.01326078277536169 1102 28-29 -0.004587420817358634 1102 30-31 0.1660886423241763 1102 32-33 0.18852399099605321 1102 34-35 0.11698543084725088 1102 36-37 0.13989386253723524 1102 38-39 0.11720478913129995 1102 40-41 0.04758970758791037 1102 42-43 -0.10013367193261757 1102 44-45 0.09235911825797416 1102 46-47 0.3690001730845438 1102 48-49 0.558633130880061 1102 50-51 0.44757369537836666 1102 52-53 0.351024409232231 1102 54-55 0.4154185575832088 1102 56-57 0.3325467351250353 1102 58-59 0.2325650459087214 1102 60-61 0.25126938203241167 1102 62-63 0.09237814085624052 1102 64-65 0.10905836852553819 1102 66-67 0.1188039068845228 1102 68-69 0.06604912061842327 1102 70-71 -0.04370279267521582 1102 72-73 0.15241018540477924 1102 74-75 -0.07074852852242586 1102 76-77 -0.24026895441470586 1102 78-79 -0.031193448255962153 1102 80-81 0.2824979964538592 1102 82-83 0.032921764778528484 1102 84-85 0.19078294575635013 1102 86-87 0.06868965547457861 1102 88-89 0.1793513773333082 1102 90-91 0.20401532035502967 1102 92-93 -0.06099858731547059 1102 94-95 0.22824366068021718 1102 96-97 0.41017065324798097 1102 98-99 0.613461236706982 1102 100-101 1.254425203871417 1103 1 -0.055327606798464046 1103 2 0.06416065044096797 1103 3 0.08698202140773859 1103 4 0.04298229750376237 1103 5 -0.1673757109183498 1103 6 -0.00901028812372573 1103 7 0.012416614998358 1103 8 0.0823978671163701 1103 9 0.31725988190044774 1103 10-11 0.07173105787191503 1103 12-13 0.13892216487590048 1103 14-15 0.20044950248156113 1103 16-17 0.2342921457848277 1103 18-19 0.16799829017979562 1103 20-21 0.21753884055030426 1103 22-23 0.22595079623143732 1103 24-25 0.17828527889285084 1103 26-27 0.21581723442086798 1103 28-29 0.3730430443074795 1103 30-31 0.31893236162421346 1103 32-33 0.3596758608097943 1103 34-35 0.2658917688774096 1103 36-37 0.41911032154119 1103 38-39 0.46870684027832965 1103 40-41 0.5399541747568222 1103 42-43 0.536964768969149 1103 44-45 0.7152276381763301 1103 46-47 0.7828137777524944 1103 48-49 0.5932457061524943 1103 50-51 0.8130890867666594 1103 52-53 0.8892020733876862 1103 54-55 0.7218258086498324 1103 56-57 0.9293158600539044 1103 58-59 0.8540774589579243 1103 60-61 0.9438471741545342 1103 62-63 0.9748427447682886 1103 64-65 0.8858719137399476 1103 66-67 0.8460027676415152 1103 68-69 0.9044629002496407 1103 70-71 0.7277981543346925 1103 72-73 0.6029137002412632 1103 74-75 0.9384710164032164 1103 76-77 0.736822323751408 1103 78-79 0.42057062312225924 1103 80-81 0.5825387645935542 1103 82-83 0.5501094992307216 1103 84-85 0.5264767260670382 1103 86-87 0.5924130323222379 1103 88-89 0.6472761940890166 1103 90-91 0.6332104043968521 1103 92-93 0.5077558893943142 1103 94-95 0.40838150795257633 1103 96-97 0.2725357149522587 1103 98-99 0.5598398085197793 1103 100-101 0.9906036720651876 1104 1 0.21671760334333 1104 2 0.3285036178138334 1104 3 0.2259438954446118 1104 4 0.1360522191232505 1104 5 0.06559149565364208 1104 6 0.11199446792110734 1104 7 0.2894257390657202 1104 8 0.09324724794362993 1104 9 0.29333830675161465 1104 10-11 0.2753838721422639 1104 12-13 0.22856501805934926 1104 14-15 0.316617837644138 1104 16-17 0.4624582692202708 1104 18-19 0.3660088443865064 1104 20-21 0.2531620598050779 1104 22-23 0.08650528010633707 1104 24-25 0.1912085003649011 1104 26-27 0.37459665482640503 1104 28-29 0.528511802968687 1104 30-31 0.2993247630388325 1104 32-33 0.3023226995366386 1104 34-35 0.2885986956298865 1104 36-37 0.48891969172555605 1104 38-39 0.4164319209315366 1104 40-41 0.35615008721898533 1104 42-43 0.5066027164420177 1104 44-45 0.4798373708843968 1104 46-47 0.4261748546806885 1104 48-49 0.73590210685758 1104 50-51 0.45080513204332107 1104 52-53 0.5027318568712431 1104 54-55 0.5270829565750219 1104 56-57 0.5071669925247235 1104 58-59 0.40039900749951585 1104 60-61 0.5897449469064995 1104 62-63 0.4574524165014182 1104 64-65 0.6096118113020594 1104 66-67 0.11974211088604392 1104 68-69 0.45345348793713214 1104 70-71 0.49771201338282367 1104 72-73 0.4847560578647965 1104 74-75 0.433199456220958 1104 76-77 0.5391429742985174 1104 78-79 0.6004813225601779 1104 80-81 0.9048855234986668 1104 82-83 0.6967784663153402 1104 84-85 0.9450627173531672 1104 86-87 0.9719556648872896 1104 88-89 0.917620342158326 1104 90-91 1.0684092935444198 1104 92-93 0.8964267467018843 1104 94-95 0.821213682762167 1104 96-97 0.9327237949070266 1104 98-99 1.2136594723816003 1104 100-101 1.9364981189116754 1105 1 0.09253844199950834 1105 2 0.23299497799671443 1105 3 0.1334160485632374 1105 4 0.13309383816446996 1105 5 0.1457787477211454 1105 6 0.19222374244541385 1105 7 0.154449607821185 1105 8 0.13879118662446643 1105 9 0.1499744931296192 1105 10-11 0.058131972727217374 1105 12-13 0.08583154128935888 1105 14-15 0.2595289293315375 1105 16-17 0.30985091585062463 1105 18-19 0.3246139229404861 1105 20-21 0.4275608585231083 1105 22-23 0.32831090103016436 1105 24-25 0.3048246168175339 1105 26-27 0.360372053199292 1105 28-29 0.3400480879593957 1105 30-31 0.33254211439139425 1105 32-33 0.33369806653275447 1105 34-35 0.2489729196151842 1105 36-37 0.17046510959686856 1105 38-39 0.17625396983446961 1105 40-41 0.32786477154629523 1105 42-43 0.11646202525664506 1105 44-45 0.12801555730721503 1105 46-47 0.15512020307487262 1105 48-49 0.022730587997905616 1105 50-51 0.14720969063943556 1105 52-53 0.4604404563468023 1105 54-55 0.20589691475426974 1105 56-57 0.1039363060458669 1105 58-59 0.07051711861809196 1105 60-61 0.09866211223965848 1105 62-63 0.3737520870453537 1105 64-65 0.23749595258716738 1105 66-67 0.3290881854282759 1105 68-69 0.5588233976168695 1105 70-71 0.5646902065292352 1105 72-73 0.44171525686074276 1105 74-75 0.6337866500173241 1105 76-77 0.4268169472696748 1105 78-79 0.6033752690852019 1105 80-81 0.7717695863418506 1105 82-83 0.669742618358196 1105 84-85 0.4532626994235862 1105 86-87 0.6561008720860144 1105 88-89 0.969796825270901 1105 90-91 1.0053952188228905 1105 92-93 0.806425850222805 1105 94-95 0.8131537755477538 1105 96-97 1.0196682580281156 1105 98-99 1.0710898831931353 1105 100-101 1.4341868837876284 1106 1 0.007910639988384816 1106 2 0.10009376555233374 1106 3 0.10959840765773166 1106 4 0.14370600456743432 1106 5 -0.04945801548068829 1106 6 0.01037456807895154 1106 7 0.09154586034528478 1106 8 -0.010670774790611404 1106 9 -0.003561001682314213 1106 10-11 0.1113108148297215 1106 12-13 0.046840134172036585 1106 14-15 0.20705741929776877 1106 16-17 0.08771732260371579 1106 18-19 0.05490915212773473 1106 20-21 0.010055009010741855 1106 22-23 0.04488782175360484 1106 24-25 -0.08050017316821823 1106 26-27 -0.2834166825193023 1106 28-29 -0.12795005301749995 1106 30-31 -0.10188434201334218 1106 32-33 -0.15089651782405866 1106 34-35 0.11020034108020127 1106 36-37 -0.139132170052072 1106 38-39 0.13124040595896957 1106 40-41 0.21200071916729968 1106 42-43 0.2580413498892895 1106 44-45 0.05622972959028516 1106 46-47 0.11622422386795961 1106 48-49 0.003912349757392519 1106 50-51 0.32620258380971023 1106 52-53 0.16627190297564454 1106 54-55 0.24481838996778293 1106 56-57 0.27698280202041303 1106 58-59 0.15382277057283744 1106 60-61 0.20445378509820955 1106 62-63 -0.05495233702171021 1106 64-65 -0.06780852800818593 1106 66-67 -0.07631871626786335 1106 68-69 -0.04433781581821705 1106 70-71 0.04909684889457111 1106 72-73 -0.0764153176589879 1106 74-75 -0.0377184302441087 1106 76-77 -0.26707799752783146 1106 78-79 0.001599581494325264 1106 80-81 -0.07631500795112345 1106 82-83 0.2401298079989509 1106 84-85 0.00491469495959862 1106 86-87 -0.10627561797345564 1106 88-89 0.07560670956273796 1106 90-91 0.1105349223698937 1106 92-93 0.1317352260514255 1106 94-95 0.2834458286610335 1106 96-97 0.3355397406718943 1106 98-99 0.7533258684025377 1106 100-101 0.7534588052540983 1107 1 -0.06837624262290021 1107 2 -0.04022843615452487 1107 3 0.022925081905956546 1107 4 -0.121905009732302 1107 5 0.015305190623763565 1107 6 0.05623289099845863 1107 7 0.019534052720779016 1107 8 -0.04452977226678456 1107 9 0.07170828047696176 1107 10-11 -0.045706664548013975 1107 12-13 -0.03545505932338955 1107 14-15 0.038527794296498996 1107 16-17 0.034410769746870074 1107 18-19 -0.10839554137417906 1107 20-21 0.04256849654080952 1107 22-23 0.06877404652799868 1107 24-25 -0.08683927626729115 1107 26-27 0.03728817155015918 1107 28-29 -0.11450143967828552 1107 30-31 -0.2024199515430425 1107 32-33 -0.17413196837170375 1107 34-35 -0.21334903776552494 1107 36-37 -0.055385526620135295 1107 38-39 -0.14279968403958065 1107 40-41 -0.13323680982205843 1107 42-43 -0.2916236058825703 1107 44-45 -0.30375219575012125 1107 46-47 -0.148977444074319 1107 48-49 -0.2442394658522531 1107 50-51 -0.2462228942534992 1107 52-53 -0.27449899422796875 1107 54-55 0.010675156218034942 1107 56-57 -0.24802696319638784 1107 58-59 -0.013656823367107052 1107 60-61 0.07492665220910766 1107 62-63 0.11007406149426657 1107 64-65 0.2660617120792139 1107 66-67 0.194757216040081 1107 68-69 0.13755898565889169 1107 70-71 0.07418012349246794 1107 72-73 0.03233244282727199 1107 74-75 0.047605061480322064 1107 76-77 0.06600381841332847 1107 78-79 0.04987074174621853 1107 80-81 0.4235826554595121 1107 82-83 0.19008577443831243 1107 84-85 -0.01682102007768549 1107 86-87 -0.12407569840136645 1107 88-89 -0.033625348022493284 1107 90-91 -0.11293431683683863 1107 92-93 0.17679503530178664 1107 94-95 0.3472901675018072 1107 96-97 0.5234253946675622 1107 98-99 0.8700414692552485 1107 100-101 0.9431540872490594 1108 1 0.04631622367185528 1108 2 -0.08257473432112761 1108 3 -0.0935604701000301 1108 4 -2.757146707281777E-4 1108 5 -0.012989730796896026 1108 6 0.022961831633018903 1108 7 0.006735379690894661 1108 8 0.1015756484581658 1108 9 -0.11031520446675103 1108 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-0.15991736503124088 2316 72-73 -0.35156147909122026 2316 74-75 -0.6754745282976451 2316 76-77 -0.5614052173808766 2316 78-79 -0.6355161662429865 2316 80-81 -1.4176091046112056 2316 82-83 -0.6046832702937657 2316 84-85 -0.8717720096512593 2316 86-87 -0.7891667365417625 2316 88-89 -0.4637045730564182 2316 90-91 -1.2839705185041446 2316 92-93 -1.0872763625222355 2316 94-95 -1.0181053169012806 2316 96-97 -0.5977115223581002 2316 98-99 -0.6597985362652032 2316 100-101 -1.1441676071348716 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 7.0 5 22.0 6 65.0 7 145.0 8 339.0 9 630.0 10 1140.0 11 1804.0 12 2640.0 13 3572.0 14 4734.0 15 6817.0 16 8845.0 17 11445.0 18 9680.0 19 5617.0 20 4709.0 21 4897.0 22 5169.0 23 5947.0 24 6873.0 25 8065.0 26 9689.0 27 11995.0 28 14980.0 29 18332.0 30 22257.0 31 27807.0 32 34532.0 33 43095.0 34 54739.0 35 74053.0 36 104403.0 37 156731.0 38 114681.0 39 6607.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.129462214715026 24.222897530633542 16.52031453950872 20.12732571514271 2 14.47951899692766 23.96184604750566 40.25300436328336 21.30563059228332 3 18.082723341969142 28.753813372501536 32.84030021612816 20.323163069401158 4 11.531986638934875 20.593777118223063 41.26874214643554 26.605494096406513 5 10.486607772459822 40.715141520161716 36.779995451432605 12.018255255945855 6 25.69298420591894 41.92489330641483 18.475682502252035 13.9064399854142 7 23.732541194078777 36.160835100442526 23.943069649724865 16.16355405575383 8 23.49329853748156 35.94852788898531 21.60565745991129 18.95251611362184 9 24.219527224908045 18.800719136527945 24.352427721061424 32.62732591750259 10-11 22.128455963210083 29.05737592964227 29.42049783784917 19.393670269298475 12-13 24.23809859597833 27.87619249092033 29.25810658774008 18.627602325361263 14-15 21.70952616007781 29.439672396300864 28.704203108194733 20.146598335426596 16-17 18.119773557624196 32.086028281791286 30.33904733347013 19.455150827114384 18-19 17.584144678915518 30.81815144072868 33.39169346446999 18.206010415885814 20-21 18.797700831060126 29.364399727088035 33.22048821764856 18.617411224203277 22-23 18.77869074285598 29.23034799258504 32.990876137306444 19.000085127252536 24-25 19.238939852107844 30.059588849642978 32.95263385256524 17.748837445683936 26-27 18.731770970649954 29.093514960271754 34.38718378927481 17.78753027980349 28-29 17.64743252953034 29.7738521872433 34.44000510781362 18.13871017541274 30-31 18.583279228823326 29.38935807698807 33.59629607512544 18.43106661906317 32-33 18.46647794842605 29.726807476901495 33.6195658802842 18.18714869438826 34-35 19.25140994371661 29.413614621102045 33.30726385467843 18.02771158050291 36-37 19.224880176063674 28.891418928878203 33.41931516109521 18.464385733962906 38-39 18.246632782719185 30.37827191867852 33.43379923761118 17.941296060991103 40-41 18.21860650676316 29.95713149475642 33.97068576902846 17.85357622945196 42-43 18.14116059619267 30.08870976963666 32.79313801598606 18.976991618184613 44-45 18.733852016977178 29.58237432763722 32.54721865583983 19.136554999545776 46-47 19.803218660684962 30.390189393145306 31.456681233415473 18.34991071275426 48-49 19.12146804004254 30.326670164904208 31.54150303088825 19.010358764165 50-51 18.629075292862044 30.52873986735446 31.832833583208398 19.0093512565751 52-53 18.97359304691654 31.555378240040454 30.94308913132833 18.527939581714676 54-55 19.044194535749632 30.557479070393867 30.935222090787924 19.463104303068572 56-57 19.452793114554765 30.94213021638355 30.390705055514154 19.214371613547527 58-59 19.532461988237223 30.23070697368304 31.072892883506825 19.16393815457291 60-61 19.32924458539486 30.83586815376132 31.371738086343985 18.463149174499833 62-63 19.03091368816945 30.36466208403746 31.748408574006078 18.856015653787022 64-65 19.050027255711946 31.016237796874425 30.810072796258154 19.123662151155475 66-67 18.933401945275623 30.859173988450323 30.08481198421925 20.1226120820548 68-69 18.655710856048703 30.41844733530231 31.019242338863123 19.906599469785863 70-71 19.466689536442352 30.55611671208858 30.870957290693905 19.106236460775158 72-73 19.114725708669848 29.601343234371658 31.602599816658415 19.681331240300082 74-75 18.705738473022983 31.256237988596112 30.376455265958292 19.66156827242261 76-77 18.7717148307887 30.642520759521176 30.83380503234553 19.7519593773446 78-79 18.88953759109272 30.354816105631027 30.794310084029874 19.96133621924638 80-81 19.063604431097474 30.59685894475232 30.153800021219052 20.18573660293115 82-83 19.086653731946345 30.83533135373665 30.45986869607092 19.61814621824609 84-85 18.619801097053813 30.57634079060554 30.158052494418797 20.64580561792185 86-87 18.234432355441257 30.642234823698626 30.676858530709637 20.446474290150476 88-89 19.131083372565012 30.337684714510893 29.959980508048687 20.5712514048754 90-91 18.923589601857767 29.62015585141699 30.760164809855556 20.696089736869695 92-93 19.23960937976459 30.26741426277165 30.687079445427006 19.805896912036754 94-95 18.92797607249404 29.529543456274364 30.71721530181286 20.825265169418735 96-97 18.84529548947124 29.67930592198674 30.33497510032609 21.140423488215934 98-99 18.702344876657328 30.179534582324802 29.593220123628555 21.524900417389315 100-101 18.204510007591857 29.99571171274193 29.916680166830258 21.883098112835956 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 735.0 1 497.5 2 264.0 3 296.5 4 287.0 5 276.5 6 405.5 7 731.0 8 1074.5 9 1329.0 10 1334.0 11 1121.5 12 1082.0 13 1108.0 14 1140.5 15 1202.0 16 1306.5 17 1424.0 18 1544.0 19 1775.0 20 2006.0 21 2251.0 22 2599.0 23 3005.5 24 3472.0 25 4480.0 26 6771.5 27 9497.0 28 10643.5 29 11989.0 30 14738.5 31 17718.5 32 22212.5 33 28002.0 34 34298.5 35 38682.5 36 44403.5 37 50058.5 38 48524.0 39 44480.0 40 39978.0 41 34745.0 42 30611.5 43 28291.5 44 27000.0 45 26534.5 46 24613.0 47 24930.5 48 34514.0 49 31544.5 50 17273.0 51 10632.0 52 7458.0 53 5964.5 54 4961.0 55 4190.0 56 3399.0 57 2776.5 58 2286.0 59 1875.5 60 1505.5 61 1136.5 62 819.5 63 536.0 64 296.0 65 180.0 66 138.0 67 110.5 68 69.5 69 52.5 70 51.0 71 35.0 72 25.5 73 21.5 74 18.0 75 19.0 76 15.5 77 12.0 78 13.0 79 13.5 80 11.0 81 6.5 82 3.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.033796360411502455 2 0.005717429392923347 3 0.004319835541319862 4 5.082159460376308E-4 5 7.623239190564463E-4 6 0.0 7 0.0 8 0.0 9 0.0015246478381128925 10-11 4.4468895278292704E-4 12-13 8.258509123111501E-4 14-15 0.0023504987504240427 16-17 0.0029857686829710814 18-19 0.0 20-21 0.0 22-23 0.001270539865094077 24-25 8.893779055658541E-4 26-27 0.00412925456155575 28-29 0.004764524494102789 30-31 0.001270539865094077 32-33 3.8116195952822313E-4 34-35 0.008576144089385021 36-37 0.01003726493424321 38-39 0.008512617096130316 40-41 0.0011434858785846693 42-43 7.623239190564463E-4 44-45 0.001334066858348781 46-47 6.352699325470386E-5 48-49 5.082159460376308E-4 50-51 8.893779055658541E-4 52-53 0.002604606723442858 54-55 0.003049295676225785 56-57 0.0019693367908958192 58-59 0.0010164318920752617 60-61 0.0036845656087728237 62-63 0.004700997500848085 64-65 0.009656102974714987 66-67 0.004065727568301047 68-69 0.0033669306424993044 70-71 2.541079730188154E-4 72-73 0.0014611208448581886 74-75 0.00692444226476272 76-77 0.0023504987504240427 78-79 0.003239876655989897 80-81 0.0048915784806121965 82-83 0.004828051487357494 84-85 0.006352699325470386 86-87 0.004319835541319862 88-89 0.009592575981460282 90-91 0.012641871657686068 92-93 0.001397593851603485 94-95 0.0029222416897163772 96-97 0.0010799588853299656 98-99 0.004065727568301047 100-101 0.005018632467121605 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 787067.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.427084881202987 #Duplication Level Percentage of deduplicated Percentage of total 1 73.8963993291357 21.745556154737475 2 12.491049727443857 7.351503611696356 3 4.577637256776464 4.041195603315546 4 2.2159210691782305 2.608323895710154 5 1.2887153258002886 1.8961567640016126 6 0.8108914523230308 1.4317302958171074 7 0.5421208352231661 1.1167125083786535 8 0.4246045668648625 0.9995899704062989 9 0.345288981109596 0.9144763340090557 >10 2.5280027650761943 15.458092783145103 >50 0.4596109267305739 9.52066355718388 >100 0.3859692136482921 22.269483069380634 >500 0.024691633196355388 4.792009110259745 >1k 0.00823054439878513 4.449103584972484 >5k 8.663730946089609E-4 1.4054027569858927 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5645 0.7172197538456065 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5380 0.6835504474206135 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4947 0.6285360712620399 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2885 0.36655075107964125 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2720 0.345586843305589 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2407 0.30581894552814437 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1814 0.23047593152806556 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1683 0.2138318592953332 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1669 0.21205310348420148 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1639 0.20824148388891925 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1619 0.20570040415873106 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1576 0.20023708273882654 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1564 0.1987124349007137 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1549 0.19680662510307254 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1509 0.19172446564269624 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1471 0.18689641415533872 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1331 0.16910885604402168 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1281 0.1627561567185513 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1154 0.1466203004318565 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1077 0.1368371434706321 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1007 0.12794336441497356 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 918 0.11663555961563628 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 887 0.11269688603384465 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 885 0.11244277806082582 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 865 0.10990169833063766 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 854 0.10850410447903419 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 850 0.10799588853299656 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 845 0.10736061860044951 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 836 0.10621713272186484 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 795 0.10100791927497912 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 794 0.10088086528846972 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 792 0.1006267573154509 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.9058097976411157E-4 0.0 22-23 0.0 0.0 0.0 6.987969258017424E-4 0.0 24-25 0.0 0.0 0.0 0.0016517018246223003 0.0 26-27 0.0 0.0 0.0 0.0028587146964616735 0.0 28-29 0.0 0.0 0.0 0.006289172332215682 0.0 30-31 0.0 0.0 0.0 0.013594776556506625 0.0 32-33 0.0 0.0 0.0 0.027126026119758548 0.0 34-35 0.0 0.0 0.0 0.04065727568301047 0.0 36-37 0.0 0.0 0.0 0.054760268185554725 0.0 38-39 0.0 0.0 0.0 0.06657628893092964 0.0 40-41 0.0 0.0 0.0 0.07635944589215404 0.0 42-43 0.0 0.0 0.0 0.08963658748238715 0.0 44-45 0.0 0.0 0.0 0.10329489103214846 0.0 46-47 0.0 0.0 0.0 0.12495759573200249 0.0 48-49 0.0 0.0 0.0 0.14839905624298821 0.0 50-51 0.0 0.0 0.0 0.1664407223273241 0.0 52-53 0.0 0.0 0.0 0.20182525757019415 0.0 54-55 0.0 0.0 0.0 0.27087909923805725 0.0 56-57 0.0 0.0 0.0 0.38522768709652416 0.0 58-59 0.0 0.0 0.0 0.5009738688065946 0.0 60-61 0.0 0.0 0.0 0.6065557315959125 0.0 62-63 0.0 0.0 0.0 0.7296075175302739 0.0 64-65 0.0 0.0 0.0 0.8850580700245341 0.0 66-67 0.0 0.0 0.0 1.1145175696605245 0.0 68-69 0.0 0.0 0.0 1.3925752191363632 0.0 70-71 0.0 0.0 0.0 1.6209547598870235 0.0 72-73 0.0 0.0 0.0 1.8524471233071644 0.0 74-75 0.0 0.0 0.0 2.1306953537627673 0.0 76-77 0.0 0.0 0.0 2.437340150203223 0.0 78-79 0.0 0.0 0.0 2.689351732444633 0.0 80-81 0.0 0.0 0.0 2.9447937723217974 0.0 82-83 0.0 0.0 0.0 3.2221526248718346 0.0 84-85 0.0 0.0 0.0 3.657249001673301 0.0 86-87 0.0 0.0 0.0 4.235471694277615 0.0 88-89 0.0 0.0 0.0 4.874045030473899 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCGTA 15 6.6176185E-4 94.99828 7 ACCGTAT 20 0.0020739818 71.24871 8 GTACCGT 25 0.0050210487 56.99897 6 GGTATGC 80 1.0773099E-5 35.624355 8 GTACATG 3500 0.0 26.334787 1 GTAGATA 290 0.0 24.568521 6 ACATGGG 3510 0.0 24.090944 3 AATGGCG 100 0.001653358 23.74957 7 ATTGCCG 100 0.001653358 23.74957 8 GGATGAC 325 0.0 23.384193 8 GAGTACT 4755 0.0 23.175186 12-13 CATATAG 290 3.6379788E-12 22.933535 3 GGACCAT 145 2.3334593E-5 22.93062 6 CATTCCG 295 5.456968E-12 22.541967 9 TACATGG 3975 0.0 22.348349 2 GTACTTT 4905 0.0 22.321207 14-15 GACATAT 280 6.548362E-11 22.058779 1 CATGGGA 1885 0.0 21.924074 4 TACCTGG 175 4.0427385E-6 21.716652 2 CATGGGG 1100 0.0 21.59189 4 >>END_MODULE