##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936644_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 798587 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45181051031384 31.0 31.0 34.0 30.0 34.0 2 31.52961042441212 31.0 31.0 34.0 30.0 34.0 3 31.438513274070328 31.0 31.0 34.0 28.0 34.0 4 35.119626289934594 37.0 35.0 37.0 32.0 37.0 5 35.17813713471419 37.0 35.0 37.0 33.0 37.0 6 35.25885720654105 37.0 35.0 37.0 33.0 37.0 7 35.14987221179408 37.0 35.0 37.0 32.0 37.0 8 35.14521899304647 37.0 35.0 37.0 32.0 37.0 9 36.63348389092234 39.0 37.0 39.0 32.0 39.0 10-11 36.5308714016131 39.0 37.0 39.0 32.0 39.0 12-13 36.48644981698926 39.0 37.0 39.0 32.0 39.0 14-15 37.44108531694104 40.0 37.0 41.0 32.0 41.0 16-17 37.372212420187154 39.0 37.0 41.0 32.0 41.0 18-19 37.372427174496956 39.5 37.0 41.0 32.0 41.0 20-21 37.407983100150645 40.0 37.0 41.0 32.0 41.0 22-23 37.42548776776982 39.5 37.0 41.0 32.0 41.0 24-25 37.53083947021426 40.0 37.0 41.0 32.0 41.0 26-27 37.46915426872714 40.0 37.0 41.0 32.0 41.0 28-29 37.38312669752951 39.5 37.0 41.0 32.0 41.0 30-31 37.202393727921944 39.0 36.0 41.0 31.0 41.0 32-33 37.021355218654946 39.0 36.0 41.0 31.0 41.0 34-35 36.83803517963604 39.0 35.0 41.0 31.0 41.0 36-37 36.63853593910244 39.0 35.0 41.0 30.0 41.0 38-39 36.468111176365255 39.0 35.0 40.0 30.0 41.0 40-41 36.292877294521446 39.0 35.0 40.0 30.0 41.0 42-43 36.038364010433426 39.0 35.0 40.0 30.0 41.0 44-45 35.80027786578043 38.5 35.0 40.0 29.0 41.0 46-47 35.52358665993812 39.0 35.0 40.0 27.0 41.0 48-49 35.21589632688736 39.0 35.0 40.0 25.5 41.0 50-51 34.25156557770161 38.0 34.0 39.5 24.0 40.5 52-53 34.39860528658744 38.0 34.0 39.5 24.0 40.5 54-55 34.89968218866573 39.0 35.0 40.0 24.0 41.0 56-57 34.8007449407516 39.0 35.0 40.0 23.5 41.0 58-59 34.63966543407293 39.0 34.0 40.0 23.0 41.0 60-61 34.39119281931712 38.0 34.0 40.0 22.5 41.0 62-63 34.10188057155952 38.0 34.0 40.0 22.0 41.0 64-65 33.79081803234964 38.0 33.5 40.0 21.0 41.0 66-67 33.435451616417495 37.5 33.0 40.0 19.5 41.0 68-69 33.00920187781669 37.0 32.5 39.0 18.0 41.0 70-71 32.608405846826955 36.0 32.0 39.0 15.0 40.5 72-73 32.183229879775155 36.0 32.0 39.0 12.5 40.0 74-75 31.751800367398918 35.0 31.0 38.0 8.0 40.0 76-77 31.2875541425042 35.0 31.0 37.0 7.0 39.0 78-79 30.791480452348964 35.0 31.0 37.0 4.0 39.0 80-81 30.300480724078902 35.0 30.0 36.0 2.0 38.5 82-83 29.87824244571975 34.0 30.0 36.0 2.0 37.5 84-85 29.463010918033977 34.0 29.5 35.0 2.0 37.0 86-87 29.142839164674605 34.0 29.0 35.0 2.0 36.5 88-89 28.768002734830393 34.0 29.0 35.0 2.0 36.0 90-91 28.410608988125276 34.0 29.0 35.0 2.0 36.0 92-93 28.02915587155814 34.0 29.0 35.0 2.0 35.5 94-95 27.587204024107578 34.0 27.0 35.0 2.0 35.0 96-97 27.04834163341001 34.0 26.0 35.0 2.0 35.0 98-99 26.42930325687746 34.0 25.0 35.0 2.0 35.0 100-101 23.90654180446213 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.24595012301402974 1101 2 0.2748501345229535 1101 3 0.6104892858596109 1101 4 0.5111757430190451 1101 5 0.4068892775553081 1101 6 0.3442786112360423 1101 7 0.4325054274372562 1101 8 0.44540037135733 1101 9 0.5859138305015676 1101 10-11 0.5498131772955261 1101 12-13 0.7091130791270075 1101 14-15 1.0041866963626944 1101 16-17 0.8198589569343611 1101 18-19 1.0417174321400182 1101 20-21 0.8562759644449969 1101 22-23 0.7302139314551823 1101 24-25 0.6569806427989349 1101 26-27 0.688647590698757 1101 28-29 0.7532647303186266 1101 30-31 0.6505025393559336 1101 32-33 0.6258618905195448 1101 34-35 0.712639733428091 1101 36-37 0.6291445952201755 1101 38-39 0.8047151513375397 1101 40-41 0.7281185274817545 1101 42-43 0.7365519159590477 1101 44-45 0.7995578572613624 1101 46-47 0.7708940834417533 1101 48-49 0.8106882378215161 1101 50-51 0.5922346032587171 1101 52-53 0.8300033961099658 1101 54-55 0.9541195109002771 1101 56-57 0.9218125479997354 1101 58-59 0.8778268923362091 1101 60-61 0.8341196107824658 1101 62-63 0.9208490100016391 1101 64-65 0.8024910239252065 1101 66-67 1.0381456961935598 1101 68-69 0.7843177376395332 1101 70-71 0.6751948464205597 1101 72-73 0.8223526895305397 1101 74-75 0.777036733852885 1101 76-77 1.0512901959138112 1101 78-79 0.992910015724604 1101 80-81 1.2776959888045774 1101 82-83 1.0383841296213596 1101 84-85 1.2734644716054397 1101 86-87 1.3281259001427443 1101 88-89 1.3033263062355864 1101 90-91 1.2019793387873072 1101 92-93 1.2215128074348414 1101 94-95 1.6457099265501682 1101 96-97 1.442271298430196 1101 98-99 1.1844495363253884 1101 100-101 1.8878302355194734 1102 1 0.1350893104106703 1102 2 0.34017658416036056 1102 3 0.31545016332631803 1102 4 0.18078901721570872 1102 5 0.3687163203125223 1102 6 0.35602705831397685 1102 7 0.3743022590671998 1102 8 0.17677991544395155 1102 9 0.4028326461318841 1102 10-11 0.2519713445260976 1102 12-13 0.2127470336291708 1102 14-15 0.3865685544931097 1102 16-17 0.30932158454277925 1102 18-19 0.5070367234348936 1102 20-21 0.705073508987411 1102 22-23 0.28870051175425715 1102 24-25 0.4356636830784879 1102 26-27 0.3448857209981213 1102 28-29 0.10753256335686245 1102 30-31 0.16761389979163255 1102 32-33 0.3178953857190763 1102 34-35 -0.11562797689569493 1102 36-37 0.0028392933718706104 1102 38-39 -0.1629196446350889 1102 40-41 0.04040706419966966 1102 42-43 0.12458082981935092 1102 44-45 0.2759090309373562 1102 46-47 0.14792544574738997 1102 48-49 0.34139898819112346 1102 50-51 0.4069832723913507 1102 52-53 0.31022445741459137 1102 54-55 0.5508761973765672 1102 56-57 0.38061336492731357 1102 58-59 0.3995093657919071 1102 60-61 0.5153630002722664 1102 62-63 0.4732561122461476 1102 64-65 0.4571944932378287 1102 66-67 0.13407259929005733 1102 68-69 0.3371930539214034 1102 70-71 0.04779826255691688 1102 72-73 0.07784708264917839 1102 74-75 0.0930877830613781 1102 76-77 0.04228602362273648 1102 78-79 -0.03626899543072071 1102 80-81 0.3051460411043081 1102 82-83 0.32536865591438513 1102 84-85 0.34322398051392256 1102 86-87 0.2054406743773889 1102 88-89 0.37376220546950023 1102 90-91 0.6643673618576216 1102 92-93 0.5053885678261274 1102 94-95 0.43835100433359386 1102 96-97 0.47946546189752226 1102 98-99 0.5996049161850294 1102 100-101 0.9819251872810106 1103 1 0.456528262180921 1103 2 0.44018228589827757 1103 3 0.4542499335879988 1103 4 0.4848112456495812 1103 5 0.4283175602319247 1103 6 0.432281544955913 1103 7 0.6745098857674634 1103 8 0.34331933600831377 1103 9 0.488162630737186 1103 10-11 0.37659881939325857 1103 12-13 0.35758716941396784 1103 14-15 0.6403007591604677 1103 16-17 0.6872452897267678 1103 18-19 0.709732811069216 1103 20-21 0.8523236720432052 1103 22-23 0.6385250790132062 1103 24-25 0.5467481397237322 1103 26-27 0.3693781620233736 1103 28-29 0.40947087611526456 1103 30-31 0.4172241651449511 1103 32-33 0.6261362048780299 1103 34-35 0.49035342431335494 1103 36-37 0.5462496836071509 1103 38-39 0.46550018128111503 1103 40-41 0.6734397351579346 1103 42-43 0.719059322446725 1103 44-45 0.7895410518975012 1103 46-47 0.8281997966879615 1103 48-49 0.9914527375009996 1103 50-51 0.926620196846109 1103 52-53 0.9945414796054806 1103 54-55 0.8238469001783457 1103 56-57 0.7140461310072581 1103 58-59 0.7616302176037308 1103 60-61 0.869055709974532 1103 62-63 0.9858131470086633 1103 64-65 0.9890060188143011 1103 66-67 0.6569303103343245 1103 68-69 0.9066763791193253 1103 70-71 1.0547203114555757 1103 72-73 1.1060630366681394 1103 74-75 0.9717450655018318 1103 76-77 0.9883733102769163 1103 78-79 0.8588475355763023 1103 80-81 1.205077759316076 1103 82-83 1.094128416663903 1103 84-85 1.0042097692798286 1103 86-87 0.9437190832865596 1103 88-89 0.9907284915103958 1103 90-91 1.2263153536638036 1103 92-93 1.0911795588020823 1103 94-95 0.981604249109207 1103 96-97 1.063004266396053 1103 98-99 1.2888066589698965 1103 100-101 1.782554881653951 1104 1 0.04807152774944967 1104 2 0.2275753848010389 1104 3 0.15530098139433335 1104 4 0.4495993883392444 1104 5 0.2144532375108099 1104 6 0.19025827117565797 1104 7 0.18270406083944835 1104 8 0.25010401032125174 1104 9 0.3169642039323648 1104 10-11 0.3588470244272628 1104 12-13 0.44330186031245233 1104 14-15 0.4670223868124026 1104 16-17 0.42731962752073116 1104 18-19 0.2973586207622958 1104 20-21 0.3799133393059506 1104 22-23 0.1363397855788122 1104 24-25 0.2710756690185434 1104 26-27 -0.0528906263678266 1104 28-29 0.037409812155587474 1104 30-31 -0.06908668676832974 1104 32-33 -0.173379329884078 1104 34-35 -0.07184302519259234 1104 36-37 0.11035387537273067 1104 38-39 -0.19131218061860267 1104 40-41 -0.04116877587117784 1104 42-43 0.21585431545054234 1104 44-45 0.35058677828472185 1104 46-47 0.26104186669298457 1104 48-49 0.007573666075124663 1104 50-51 0.13984702976435415 1104 52-53 0.06518534430637146 1104 54-55 -0.009192899714683733 1104 56-57 0.38268176776772833 1104 58-59 0.4035101912397536 1104 60-61 0.20286265861338393 1104 62-63 0.19794260606075653 1104 64-65 0.2709480396569788 1104 66-67 0.11160032832449929 1104 68-69 -0.1228608735157124 1104 70-71 -0.09158967606474988 1104 72-73 0.25690298350797036 1104 74-75 -0.006293951035086565 1104 76-77 -0.042067503116854255 1104 78-79 -0.23259848578501163 1104 80-81 -0.09680742768993866 1104 82-83 -0.10046061851148735 1104 84-85 -0.30533339904449264 1104 86-87 -0.21203777378066135 1104 88-89 -0.35615645238509686 1104 90-91 -0.22980995089965006 1104 92-93 -0.3077458927081729 1104 94-95 -0.27249283842678906 1104 96-97 -0.35446714110580047 1104 98-99 -0.013329742238092024 1104 100-101 0.7455689928689075 1105 1 0.30586914903236817 1105 2 0.43573861178773043 1105 3 0.26985519994231666 1105 4 0.46568952637350236 1105 5 0.34397100278282977 1105 6 0.36705397725334876 1105 7 0.42799651857886545 1105 8 0.42741204720907433 1105 9 0.33142212574692564 1105 10-11 0.47221765150944606 1105 12-13 0.5630122458749227 1105 14-15 0.7231153681485338 1105 16-17 0.7311961745720126 1105 18-19 0.5847312757088332 1105 20-21 0.4950234770183215 1105 22-23 0.45126229219083314 1105 24-25 0.3226346568030749 1105 26-27 0.4648243513954142 1105 28-29 0.46386016434949084 1105 30-31 0.2889970082365849 1105 32-33 0.47771735310922026 1105 34-35 0.39233874763294807 1105 36-37 0.61651391571543 1105 38-39 0.4586954641081462 1105 40-41 0.5868133381505913 1105 42-43 0.22637855594023648 1105 44-45 0.388371175531546 1105 46-47 0.5690412014676411 1105 48-49 0.7950583095626271 1105 50-51 0.864725384726448 1105 52-53 1.0126177890631638 1105 54-55 0.9321921638304289 1105 56-57 0.8790639343637636 1105 58-59 1.020606616726461 1105 60-61 0.7116608002742026 1105 62-63 0.9065946932052711 1105 64-65 1.1788666353877275 1105 66-67 1.0897142803531708 1105 68-69 0.92337564091806 1105 70-71 1.068109336290206 1105 72-73 1.1274502654723122 1105 74-75 0.9701441964196214 1105 76-77 0.7744396078202023 1105 78-79 1.0402793648933617 1105 80-81 1.1751423263376246 1105 82-83 1.2792724061715894 1105 84-85 1.2383501790095117 1105 86-87 1.380647161547632 1105 88-89 1.2131517132560141 1105 90-91 1.031010690399043 1105 92-93 1.2032786079704643 1105 94-95 1.1019505718187546 1105 96-97 1.3837888150751638 1105 98-99 1.5588861019275058 1105 100-101 1.9033447529156895 1106 1 0.08002237260351919 1106 2 0.08732960907139287 1106 3 0.0860229476001706 1106 4 0.28055176929162684 1106 5 0.21817827591326733 1106 6 0.11291572125246319 1106 7 0.40106043564743743 1106 8 0.30164010863154544 1106 9 0.4838612853455757 1106 10-11 0.492218760218357 1106 12-13 0.5872534732156751 1106 14-15 0.2959970411902759 1106 16-17 0.30633268435637007 1106 18-19 0.4809415700710531 1106 20-21 0.386345750980297 1106 22-23 0.1388551465619159 1106 24-25 -0.07871311746379206 1106 26-27 -0.09031382145231248 1106 28-29 0.05453730831533932 1106 30-31 0.16472667728697132 1106 32-33 0.2560753552157635 1106 34-35 0.23946649861692748 1106 36-37 -0.004373467177195778 1106 38-39 0.014505791731629358 1106 40-41 0.03928437313036426 1106 42-43 -0.06045136811320617 1106 44-45 -0.019612914495610312 1106 46-47 0.31163710478821827 1106 48-49 0.21360285194302975 1106 50-51 -0.1602489763800392 1106 52-53 -0.06693831160760766 1106 54-55 0.11622952732064817 1106 56-57 0.2962378353560595 1106 58-59 0.30210850153161317 1106 60-61 0.28713685216177254 1106 62-63 0.33365689177504265 1106 64-65 0.15643191062471118 1106 66-67 -0.06616464863402172 1106 68-69 0.16650164568550707 1106 70-71 0.2126537955174328 1106 72-73 0.200144150942684 1106 74-75 0.47707778475600193 1106 76-77 0.27150776570034907 1106 78-79 0.29514728994463724 1106 80-81 0.3657501759966948 1106 82-83 0.5106952954670056 1106 84-85 0.42174033367440344 1106 86-87 0.6805889543299379 1106 88-89 0.6545578333291857 1106 90-91 0.5538882057823784 1106 92-93 0.6078839075990459 1106 94-95 0.24035278369302304 1106 96-97 0.31068262847945505 1106 98-99 0.4516088138294947 1106 100-101 1.2923047839595405 1107 1 -0.009910793645435234 1107 2 -0.006530040051416108 1107 3 0.17495360758346123 1107 4 0.08113656461326713 1107 5 0.16615237783474157 1107 6 0.004979213315955633 1107 7 0.12177889847116319 1107 8 0.2264521387067333 1107 9 0.19551542068391825 1107 10-11 0.19146688051910843 1107 12-13 0.09597318917223419 1107 14-15 -0.006624094983486373 1107 16-17 0.14212006948586264 1107 18-19 0.2920422384085626 1107 20-21 0.10732528314403567 1107 22-23 -0.05752121099865093 1107 24-25 0.07871169456628024 1107 26-27 0.07268743168052083 1107 28-29 0.06082385802294965 1107 30-31 -0.06606906206140195 1107 32-33 0.11171445296012905 1107 34-35 0.020491978984509274 1107 36-37 -0.026710559909027154 1107 38-39 0.18368916683605363 1107 40-41 0.14014068056561513 1107 42-43 0.1271635023963995 1107 44-45 0.08170287497806328 1107 46-47 0.5080761189903953 1107 48-49 0.5371826347333339 1107 50-51 0.08873807458068939 1107 52-53 0.25556586550182914 1107 54-55 0.4191952750232346 1107 56-57 0.6426037501430244 1107 58-59 0.645435569200778 1107 60-61 0.5637137109754775 1107 62-63 0.8341372593606664 1107 64-65 0.8303667477174415 1107 66-67 0.7402680999039859 1107 68-69 0.7904364827782402 1107 70-71 0.9736291505717389 1107 72-73 0.7855451534489575 1107 74-75 0.7712089459924236 1107 76-77 0.6794964875048635 1107 78-79 0.6396857193514904 1107 80-81 0.644175405737716 1107 82-83 0.5315703998947434 1107 84-85 0.6364228733569419 1107 86-87 0.7446240420492352 1107 88-89 0.6580039486174059 1107 90-91 0.5926831097088616 1107 92-93 0.8359331974904407 1107 94-95 0.49773582462869825 1107 96-97 0.6088342575521395 1107 98-99 0.7572270260266549 1107 100-101 1.1785620939010606 1108 1 -0.07986773728659102 1108 2 -0.08733461559283384 1108 3 0.08376409534131923 1108 4 0.17884236758222727 1108 5 0.1958034285384258 1108 6 0.13660314493988324 1108 7 0.5096074441944438 1108 8 0.30872191571335605 1108 9 -0.02267107118678524 1108 10-11 0.15851912651871913 1108 12-13 -0.07701697855477363 1108 14-15 0.3674714001646393 1108 16-17 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-4.648922565527897 2316 82-83 -3.9940739579133613 2316 84-85 -4.0213274695785834 2316 86-87 -4.575446710964986 2316 88-89 -6.351894253678456 2316 90-91 -5.345227510462966 2316 92-93 -4.781783934846576 2316 94-95 -4.544643786951692 2316 96-97 -5.825127189274806 2316 98-99 -7.771500141686868 2316 100-101 -8.340685613845675 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1516.0 3 457.0 4 410.0 5 440.0 6 514.0 7 637.0 8 791.0 9 1042.0 10 1395.0 11 1948.0 12 2799.0 13 3760.0 14 4503.0 15 6247.0 16 8247.0 17 11200.0 18 8281.0 19 5282.0 20 4842.0 21 5173.0 22 5505.0 23 5999.0 24 7028.0 25 7956.0 26 9574.0 27 10994.0 28 13267.0 29 16078.0 30 19638.0 31 24220.0 32 30306.0 33 39236.0 34 50933.0 35 69806.0 36 98168.0 37 146407.0 38 148769.0 39 25219.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.22125008301891 23.21734913428512 15.870081991350995 20.691318791344983 2 14.253236952298401 23.05666999432586 40.9556352468 21.734457806575737 3 17.74444433311959 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31.447467445381243 18.799658394076495 48-49 18.927307129288398 30.17985697094464 31.504146740648075 19.388689159118886 50-51 18.35692018330185 30.330583221206314 31.945337529983153 19.367159065508684 52-53 18.711670461923564 31.366749542471712 31.048811991717844 18.87276800388688 54-55 18.760718196656956 30.400452801742084 31.09280369554342 19.74602530605754 56-57 19.21473334506264 30.797632516643823 30.510494077221832 19.477140061071704 58-59 19.349239911665833 29.938007046446817 31.199091863651816 19.513661178235534 60-61 19.269163315281876 30.500262663413274 31.506210314814155 18.7243637064907 62-63 18.759149223920407 30.345369506552377 31.80317193966678 19.092309329860438 64-65 18.801513182115297 30.80497682141596 31.11934654174524 19.274163454723496 66-67 18.62356112236369 30.60654346920951 30.24396352286708 20.525931885559725 68-69 18.39757111738493 30.328974843984113 31.275745673599804 19.99770836503115 70-71 19.409856511996153 30.235352383671533 30.954952518162187 19.39983858617013 72-73 18.92960226749673 29.43311955521794 31.70788069089513 19.929397486390197 74-75 18.430905473720994 31.132159950304366 30.560747839122914 19.87618673685173 76-77 18.48213072343218 30.33772109186487 31.221430982514303 19.958717202188637 78-79 18.719651416340298 30.001527996783693 31.127611528109504 20.151209058766504 80-81 18.78112513549003 30.34989145143242 30.6273524857215 20.241630927356056 82-83 18.897811338711847 30.45034891474834 31.003227735357008 19.648612011182802 84-85 18.686353868733065 30.17239867961671 30.639491782667715 20.50175566898251 86-87 18.23818566708554 30.35671687488143 31.182375595514255 20.222721862518775 88-89 19.04200787275859 29.852681615349315 30.601764514733404 20.503545997158696 90-91 18.814406201278576 29.284573818632637 31.34732544815884 20.55369453192995 92-93 19.36018734776429 29.74076880208888 31.38763830360106 19.511405546545777 94-95 18.904224016851735 29.132610346859426 31.47082599920976 20.492339637079073 96-97 18.793401636316048 29.093388737146224 31.280612046822736 20.83259757971499 98-99 18.584298568179314 29.557853972325482 30.696847790146585 21.16099966934862 100-101 18.399538599403712 29.272010305114293 31.173784831267533 21.154666264214463 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 495.0 1 344.5 2 229.5 3 278.0 4 276.0 5 297.5 6 435.5 7 778.0 8 1259.5 9 1519.5 10 1360.5 11 1092.5 12 1057.0 13 1094.5 14 1151.0 15 1258.5 16 1314.0 17 1381.0 18 1475.0 19 1647.5 20 1934.0 21 2232.0 22 2530.0 23 2911.5 24 3391.5 25 4349.5 26 6558.5 27 9324.5 28 10510.0 29 11802.5 30 14922.5 31 17994.5 32 22238.0 33 28430.5 34 34902.0 35 39601.0 36 45656.5 37 50972.0 38 49139.0 39 44970.5 40 40318.0 41 35411.5 42 31450.5 43 28847.5 44 27604.5 45 27197.0 46 25153.5 47 25086.5 48 34321.5 49 31699.5 50 17736.5 51 10928.5 52 7714.5 53 6208.0 54 5213.0 55 4416.0 56 3608.0 57 2944.5 58 2434.5 59 1972.5 60 1546.5 61 1234.0 62 891.0 63 548.5 64 330.0 65 232.0 66 160.5 67 116.5 68 76.0 69 46.0 70 48.5 71 44.5 72 34.0 73 26.0 74 24.0 75 26.5 76 22.0 77 13.0 78 10.5 79 8.5 80 3.5 81 3.5 82 4.0 83 3.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07212739501143896 2 0.02855042719202792 3 0.015402204143067692 4 0.018532733440439177 5 0.02266503211296953 6 0.027673878988763903 7 0.07262827969901839 8 0.054220767430474076 9 0.06711854813564458 10-11 0.05622430618079182 12-13 0.03600108691977205 14-15 0.042262145514515015 16-17 0.05071457461741801 18-19 0.04720838180436195 20-21 0.0175309640652803 22-23 0.01264733836138079 24-25 0.020786714534546644 26-27 0.023729412074075834 28-29 0.005572342149321239 30-31 0.0013774328908434522 32-33 0.008139376173165853 34-35 0.009892472579693884 36-37 0.003756635156845779 38-39 0.008577650274797862 40-41 0.0033183610552137716 42-43 0.0018157069924754597 44-45 4.382741016320075E-4 46-47 7.513270313691557E-4 48-49 0.0010017693751588745 50-51 0.0016904858205806005 52-53 3.1305292973714824E-4 54-55 4.382741016320075E-4 56-57 0.0021913705081600377 58-59 0.00356880339900349 60-61 0.004570572774162364 62-63 0.0027548657816869044 64-65 6.261058594742965E-4 66-67 5.634952735268669E-4 68-69 0.003819245742793208 70-71 0.002316591680054897 72-73 0.02172587332375809 74-75 0.015840478244699703 76-77 0.023979854417865556 78-79 0.01953450281559805 80-81 0.012083843087853922 82-83 0.004946236289846942 84-85 0.0038818563287406384 86-87 0.0015652646486857414 88-89 0.0013148223048960225 90-91 0.00525928921958409 92-93 0.00901592437642987 94-95 0.011708179572169344 96-97 0.010769020782957899 98-99 0.020411051018862064 100-101 0.018595344026386604 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 798587.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.13121190585287 #Duplication Level Percentage of deduplicated Percentage of total 1 75.21510113233794 23.415372518709667 2 12.111394412245193 7.540847718459349 3 4.30099035352195 4.016851263015626 4 2.06490758140321 2.571323019306618 5 1.1824350239932997 1.8405317648418815 6 0.7399389092574056 1.382111698888676 7 0.5260588607611326 1.1463794908514457 8 0.4131498003376033 1.0289483186536588 9 0.2927226616653295 0.8201530088953782 >10 2.32664516255006 15.148592031369986 >50 0.4366314129584634 9.523532887451701 >100 0.3589535397424259 21.46832667310299 >500 0.02289453100862853 4.678720146254633 >1k 0.007767787306498966 4.765393751967193 >5k 4.0883091086836663E-4 0.6529157082311763 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5130 0.6423846118206282 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4801 0.6011868462672195 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4694 0.5877881808744695 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3517 0.44040286155422015 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2596 0.3250741622390547 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2486 0.3112998333306202 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1807 0.22627465761401072 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1780 0.22289368597284953 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1531 0.19171361417102958 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1460 0.18282291096649458 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1380 0.17280521721490583 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1368 0.17130256315216752 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1339 0.1676711491672166 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1333 0.16691982213584744 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1325 0.16591805276068855 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1299 0.16266230229142223 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1284 0.16078398471299934 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1266 0.15853000361889186 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1162 0.1455070017418265 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1014 0.12697426830138733 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 952 0.11921055564390606 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 929 0.11633046869032428 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 894 0.11194772767400421 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 864 0.10819109251715843 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 851 0.10656321728252525 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 839 0.10506056321978693 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 820 0.10268136095378463 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 819 0.10255613978188975 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 799 0.10005171634399257 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.504423437897186E-4 0.0 14-15 0.0 0.0 0.0 2.504423437897186E-4 0.0 16-17 0.0 0.0 0.0 2.504423437897186E-4 0.0 18-19 0.0 0.0 0.0 2.504423437897186E-4 0.0 20-21 0.0 0.0 0.0 3.1305292973714824E-4 0.0 22-23 0.0 0.0 0.0 7.513270313691558E-4 0.0 24-25 0.0 0.0 0.0 0.001252211718948593 0.0 26-27 0.0 0.0 0.0 0.001627875234633171 0.0 28-29 0.0 0.0 0.0 0.004069688086582927 0.0 30-31 0.0 0.0 0.0 0.009642030235904165 0.0 32-33 0.0 0.0 0.0 0.019033618128018612 0.0 34-35 0.0 0.0 0.0 0.032807947036453136 0.0 36-37 0.0 0.0 0.0 0.045893559499465934 0.0 38-39 0.0 0.0 0.0 0.05935483547816331 0.0 40-41 0.0 0.0 0.0 0.07143867856601722 0.0 42-43 0.0 0.0 0.0 0.08558867099013633 0.0 44-45 0.0 0.0 0.0 0.09817339876556969 0.0 46-47 0.0 0.0 0.0 0.11651830044816658 0.0 48-49 0.0 0.0 0.0 0.1408738183817167 0.0 50-51 0.0 0.0 0.0 0.158843056548629 0.0 52-53 0.0 0.0 0.0 0.19096228713966043 0.0 54-55 0.0 0.0 0.0 0.2552633589076707 0.0 56-57 0.0 0.0 0.0 0.3638927255264611 0.0 58-59 0.0 0.0 0.0 0.4726473133171464 0.0 60-61 0.0 0.0 0.0 0.5809636270061997 0.0 62-63 0.0 0.0 0.0 0.7011133414393171 0.0 64-65 0.0 0.0 0.0 0.8540710029088878 0.0 66-67 0.0 0.0 0.0 1.0834135792343225 0.0 68-69 0.0 0.0 0.0 1.3467537037292117 0.0 70-71 0.0 0.0 0.0 1.5746562365778556 0.0 72-73 0.0 0.0 0.0 1.8050631928643968 0.0 74-75 0.0 0.0 0.0 2.0757913665010825 0.0 76-77 0.0 0.0 0.0 2.3818319106121186 0.0 78-79 0.0 0.0 0.0 2.6324620861596797 0.0 80-81 0.0 0.0 0.0 2.8850958004575578 0.0 82-83 0.0 0.0 0.0 3.164151182025252 0.0 84-85 0.0 0.0 0.0 3.596226835648464 0.0 86-87 0.0 0.0 0.0 4.146699107298265 0.0 88-89 0.0 0.0 0.0 4.764164705911817 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGACCGT 75 3.065539E-4 31.687891 5 TGTATGG 150 3.322384E-8 28.510168 2 GACATAT 305 0.0 28.046303 1 GTACAGT 85 6.3874177E-4 27.954649 1 GACCGTG 90 8.9102105E-4 26.406578 6 CATTCCG 155 1.3992376E-6 24.537176 9 GTACATG 3840 0.0 24.008968 1 GAGTACT 3935 0.0 23.309296 12-13 CCTCATC 145 2.3188826E-5 22.949284 8 ATGCGGT 105 0.0021871033 22.634209 6 ACATGGG 3880 0.0 22.16919 3 CATATAG 365 0.0 22.133955 3 GGATGAC 485 0.0 21.563538 8 GTACTTT 4245 0.0 21.549757 14-15 CATGGGG 1105 0.0 21.506271 4 GTATGGT 155 3.8900063E-5 21.461958 1 ATGGGAT 810 0.0 21.125261 5 TACATGG 4315 0.0 20.922876 2 GGATATG 390 0.0 20.722904 9 AGTACTT 4450 0.0 20.504906 12-13 >>END_MODULE