##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936644_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 798587 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.769764596718954 31.0 31.0 34.0 30.0 34.0 2 31.932850146571383 31.0 31.0 34.0 30.0 34.0 3 31.987382714719875 31.0 31.0 34.0 30.0 34.0 4 35.71803823503263 37.0 35.0 37.0 35.0 37.0 5 35.536138204103 37.0 35.0 37.0 33.0 37.0 6 35.64407760206465 37.0 35.0 37.0 33.0 37.0 7 35.592263585557994 37.0 35.0 37.0 33.0 37.0 8 35.58374228481055 37.0 35.0 37.0 33.0 37.0 9 37.16483864625896 39.0 37.0 39.0 33.0 39.0 10-11 37.07041499548578 39.0 37.0 39.0 33.0 39.0 12-13 36.999366380870214 39.0 37.0 39.0 33.0 39.0 14-15 38.03736975432858 40.0 37.0 41.0 33.0 41.0 16-17 37.94197501336736 40.0 37.0 41.0 33.0 41.0 18-19 38.16965527863589 40.0 37.5 41.0 33.0 41.0 20-21 38.24423325198131 40.0 38.0 41.0 33.0 41.0 22-23 38.15928884392058 40.0 37.0 41.0 33.0 41.0 24-25 38.02625825364049 40.0 37.0 41.0 33.0 41.0 26-27 37.823642258138435 40.0 37.0 41.0 33.0 41.0 28-29 37.74687354039071 40.0 37.0 41.0 32.5 41.0 30-31 37.669419862832726 39.0 37.0 41.0 33.0 41.0 32-33 37.46574011347543 39.0 36.0 41.0 32.0 41.0 34-35 37.27986243202055 39.0 36.0 41.0 32.0 41.0 36-37 37.061994497781704 39.0 35.5 41.0 31.0 41.0 38-39 36.89226283423096 39.0 35.0 41.0 31.0 41.0 40-41 36.729484076249676 39.0 35.0 41.0 31.0 41.0 42-43 36.42585153527418 39.0 35.0 40.5 30.0 41.0 44-45 36.09615107683947 39.0 35.0 40.0 30.0 41.0 46-47 35.635217578047225 39.0 35.0 40.0 28.0 41.0 48-49 35.18368255431155 38.5 35.0 40.0 26.5 41.0 50-51 34.92744747910998 38.0 35.0 40.0 24.5 41.0 52-53 34.63373245494856 38.0 34.0 40.0 24.0 41.0 54-55 34.489748142656964 38.0 34.0 40.0 23.5 41.0 56-57 34.235489057547895 38.0 34.0 40.0 23.0 41.0 58-59 33.93910995295441 38.0 33.0 40.0 22.0 41.0 60-61 33.564592210992664 38.0 33.0 40.0 20.5 41.0 62-63 33.29903441954352 37.0 32.5 40.0 20.0 41.0 64-65 32.92070744953274 37.0 32.0 39.0 18.5 40.5 66-67 32.5237707350608 36.0 31.5 39.0 17.5 40.0 68-69 32.148745847352885 36.0 31.0 39.0 13.5 40.0 70-71 31.798039537332812 35.5 31.0 38.5 12.0 40.0 72-73 31.325407876662155 35.0 30.5 38.0 8.0 40.0 74-75 30.798813404175124 35.0 30.0 37.0 7.5 39.0 76-77 29.22285549351542 33.0 28.5 35.5 6.0 38.0 78-79 29.97140198876265 34.0 29.0 36.0 2.0 38.5 80-81 29.695365063543484 34.0 29.0 36.0 2.0 37.5 82-83 29.373131543588865 34.0 29.0 35.5 2.0 37.0 84-85 28.95680307843729 34.0 29.0 35.0 2.0 36.5 86-87 28.598449511449598 34.0 29.0 35.0 2.0 36.0 88-89 28.123478093182083 33.5 28.5 35.0 2.0 36.0 90-91 27.65338967451261 33.0 26.5 35.0 2.0 35.0 92-93 27.078209387330375 33.0 26.0 35.0 2.0 35.0 94-95 26.548327859081102 32.0 25.0 34.0 2.0 35.0 96-97 25.856097081470146 32.0 24.5 34.0 2.0 35.0 98-99 25.079685744947014 32.0 20.5 34.0 2.0 35.0 100-101 21.91189125292548 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32819860879739693 1101 2 0.28706202817826565 1101 3 0.21079453898177647 1101 4 0.1940504421332392 1101 5 0.17393440121041692 1101 6 0.22511162089392656 1101 7 0.2478444241487736 1101 8 0.18849076906133888 1101 9 0.2109791324239012 1101 10-11 0.29057696777469744 1101 12-13 0.3629964614563619 1101 14-15 0.5726927537899442 1101 16-17 0.5815645213783682 1101 18-19 0.5306547594887618 1101 20-21 0.4145269547419943 1101 22-23 0.3582678035278235 1101 24-25 0.5014703015740665 1101 26-27 0.35180703531003843 1101 28-29 0.4954231227327739 1101 30-31 0.6793032697780745 1101 32-33 0.5857029041859789 1101 34-35 0.6234643125166883 1101 36-37 0.5804475145100625 1101 38-39 0.6412993590011169 1101 40-41 0.6412952755093286 1101 42-43 0.4651621052610224 1101 44-45 0.25003421934273007 1101 46-47 0.4941989043536452 1101 48-49 0.5442371805422752 1101 50-51 0.5284918263918854 1101 52-53 0.7352053846040434 1101 54-55 0.8537043541558305 1101 56-57 0.8670914363650937 1101 58-59 0.6831634814303698 1101 60-61 0.6032289611431736 1101 62-63 0.728176754432937 1101 64-65 0.7548720977355714 1101 66-67 0.663738133831032 1101 68-69 0.5850796212949732 1101 70-71 0.40509626098969775 1101 72-73 0.727585097870147 1101 74-75 0.8262492133459212 1101 76-77 0.6117131149257524 1101 78-79 0.6057075540493884 1101 80-81 0.49897400229479416 1101 82-83 0.7168908929238178 1101 84-85 0.5073404532587027 1101 86-87 0.47024937815462664 1101 88-89 0.47062501148764824 1101 90-91 0.6098698294215907 1101 92-93 0.6127139208568586 1101 94-95 0.6378683359419952 1101 96-97 0.8344000036381196 1101 98-99 0.8640229489780751 1101 100-101 1.16938236663999 1102 1 0.11944673155858965 1102 2 0.12241722805671529 1102 3 0.06225016602336453 1102 4 -0.010280793367464014 1102 5 0.017181308514260252 1102 6 -0.051498421822387286 1102 7 0.12845203595039578 1102 8 0.03408239876758046 1102 9 0.2021013267918903 1102 10-11 0.18996500253210513 1102 12-13 0.2987912420020322 1102 14-15 0.22013854030186764 1102 16-17 0.20948337206995404 1102 18-19 0.24738161466819264 1102 20-21 0.10854360300149324 1102 22-23 0.2639931627597534 1102 24-25 0.35516400632311473 1102 26-27 0.3482782646165816 1102 28-29 0.2836815418201226 1102 30-31 0.25262953242204844 1102 32-33 0.267192453005876 1102 34-35 0.19990956726603315 1102 36-37 0.12187824805025826 1102 38-39 0.0523715618422429 1102 40-41 0.04267084598540549 1102 42-43 0.23995845772027735 1102 44-45 0.18676615840624322 1102 46-47 0.001255715297439508 1102 48-49 0.30026771890806714 1102 50-51 0.03826223351165936 1102 52-53 0.26310977252192913 1102 54-55 -0.026919327394338666 1102 56-57 -0.1248327845442816 1102 58-59 -0.0511524439826232 1102 60-61 0.13598422179449443 1102 62-63 0.0683411917872121 1102 64-65 0.046730140498631556 1102 66-67 0.06589381755500767 1102 68-69 -0.03592850707602224 1102 70-71 0.049638536115477194 1102 72-73 0.028807421380484755 1102 74-75 -0.0949761488077776 1102 76-77 -0.0399750852039773 1102 78-79 -0.15016813037581045 1102 80-81 -0.07679340124288814 1102 82-83 -0.16850696483261274 1102 84-85 -0.18005838036972932 1102 86-87 0.0718437893882573 1102 88-89 -0.09064893974212396 1102 90-91 -0.24773466777057962 1102 92-93 -0.2846515169123123 1102 94-95 -0.34506047799368744 1102 96-97 -0.04706880059728391 1102 98-99 0.1317690644815812 1102 100-101 0.4702423903647812 1103 1 0.16297185188611962 1103 2 0.1651538397502854 1103 3 0.2163862206154228 1103 4 0.26630773167637756 1103 5 0.25449042197745797 1103 6 0.11262540879984329 1103 7 0.06350228773079891 1103 8 0.14398687379744501 1103 9 0.19982897563158275 1103 10-11 0.20191640147436374 1103 12-13 0.2840105294516704 1103 14-15 0.2556965248904888 1103 16-17 0.21809438699321504 1103 18-19 0.21608115950262885 1103 20-21 0.11436424301462011 1103 22-23 0.0671921158650477 1103 24-25 0.303032738409577 1103 26-27 0.14441888338030395 1103 28-29 0.10435840764432669 1103 30-31 0.28270967660648694 1103 32-33 0.17403718077569863 1103 34-35 0.17004289058655786 1103 36-37 0.10111349202205844 1103 38-39 0.25841498683448805 1103 40-41 0.4184856034738047 1103 42-43 0.21546601669502508 1103 44-45 0.47670161751435813 1103 46-47 0.30193269237506115 1103 48-49 0.2611635314683056 1103 50-51 0.36200899847072066 1103 52-53 0.5355290755470534 1103 54-55 0.42812872713095373 1103 56-57 0.12633294272630025 1103 58-59 0.3318923953187607 1103 60-61 0.33562014496844483 1103 62-63 0.3278856924609954 1103 64-65 0.5897240257323872 1103 66-67 0.36667300871481245 1103 68-69 0.4562211732789905 1103 70-71 0.37663181501807586 1103 72-73 0.4313118749940621 1103 74-75 0.3386792631845097 1103 76-77 0.35180314778571997 1103 78-79 0.22716384566576409 1103 80-81 0.25326630012510876 1103 82-83 0.3056287003724627 1103 84-85 0.5294610187505349 1103 86-87 0.3417619764024806 1103 88-89 0.42218831457902795 1103 90-91 0.5678593073003064 1103 92-93 0.5933083456340853 1103 94-95 0.5084590935693782 1103 96-97 0.7018996715733685 1103 98-99 0.19846340543716678 1103 100-101 0.6235148402624979 1104 1 0.03167866600381686 1104 2 0.17268891519558238 1104 3 0.09070078401594017 1104 4 0.14792776614404346 1104 5 0.08637679752378347 1104 6 -0.18284166052987416 1104 7 0.12786984922425404 1104 8 0.03911845061227126 1104 9 0.14582737650462008 1104 10-11 0.06437324935740918 1104 12-13 0.02659093150244729 1104 14-15 0.08802725243923959 1104 16-17 0.22027022612968494 1104 18-19 0.05325924065832055 1104 20-21 -0.0035963568740839946 1104 22-23 0.0797440469729267 1104 24-25 0.07645441090189564 1104 26-27 0.2535430912451986 1104 28-29 0.16310944086402657 1104 30-31 0.1398944027829998 1104 32-33 0.11551777785513195 1104 34-35 -0.04714191662679923 1104 36-37 0.2543311947897351 1104 38-39 0.2391660362615653 1104 40-41 0.3222503049070724 1104 42-43 0.1337025470217057 1104 44-45 -0.11676222114670054 1104 46-47 0.051882044350470835 1104 48-49 0.14601693582591935 1104 50-51 0.21987510940475374 1104 52-53 0.27570673531118217 1104 54-55 0.15872580656326818 1104 56-57 0.07402771476966308 1104 58-59 0.1511437769968751 1104 60-61 0.2946519708364832 1104 62-63 0.11036728359232484 1104 64-65 0.23761085508500202 1104 66-67 0.49436343178113873 1104 68-69 0.30250374333533614 1104 70-71 0.2550684523133846 1104 72-73 0.5531055173838908 1104 74-75 0.2656461623368642 1104 76-77 0.031127543656086942 1104 78-79 0.03463652274476203 1104 80-81 0.34815166000971587 1104 82-83 0.2498044476671808 1104 84-85 0.5063472862477383 1104 86-87 0.48123380529589355 1104 88-89 0.5416960427922746 1104 90-91 0.6076848619974733 1104 92-93 0.7614307490786913 1104 94-95 0.724214275875255 1104 96-97 0.6728911843150627 1104 98-99 0.8863493433845591 1104 100-101 1.220649647745983 1105 1 -0.04376271637736551 1105 2 0.011971276530971409 1105 3 0.0345026541575173 1105 4 0.16863424618996703 1105 5 0.1918954855277306 1105 6 0.09215257239556252 1105 7 -0.06927021826393087 1105 8 0.18076182545065222 1105 9 0.12724532578655356 1105 10-11 0.05959395395979783 1105 12-13 0.15854360930269706 1105 14-15 0.12203555702661362 1105 16-17 0.07019619298644386 1105 18-19 0.13886140371364064 1105 20-21 0.04316992371152395 1105 22-23 0.3553853993116718 1105 24-25 0.015409926639300409 1105 26-27 -0.02486176275392893 1105 28-29 -0.06409519103566907 1105 30-31 0.11765093030669505 1105 32-33 0.01755529871048367 1105 34-35 0.09343454941719642 1105 36-37 0.0723558510452662 1105 38-39 0.19545429342480958 1105 40-41 0.04594439781330806 1105 42-43 -0.010946412490746127 1105 44-45 0.09526896933842721 1105 46-47 -0.09447893159948961 1105 48-49 -0.004236815454909504 1105 50-51 0.18081122631274837 1105 52-53 0.39244372452272813 1105 54-55 0.21516400766518018 1105 56-57 0.12393436863253982 1105 58-59 0.17629761035792058 1105 60-61 0.19147095980125073 1105 62-63 0.19712621451081702 1105 64-65 0.18523777096082483 1105 66-67 0.13306895154208576 1105 68-69 -0.0938029148193067 1105 70-71 -0.06970904590284377 1105 72-73 0.05190180821612245 1105 74-75 -0.21272596686706535 1105 76-77 -0.09958051898238907 1105 78-79 -0.2669519710026549 1105 80-81 -0.01955768479696829 1105 82-83 0.08632464852231791 1105 84-85 -0.07149950269879213 1105 86-87 0.04318760968745394 1105 88-89 0.10238980845055323 1105 90-91 0.2462381578135293 1105 92-93 0.2808195196456893 1105 94-95 0.5848388366387631 1105 96-97 0.812293447154179 1105 98-99 0.7113085715085674 1105 100-101 1.4518328905049493 1106 1 0.18094901930642848 1106 2 0.10594544361340397 1106 3 0.1160080036780009 1106 4 0.1422442023959647 1106 5 0.27393440121041834 1106 6 0.04469290168210449 1106 7 0.03823851281872237 1106 8 0.0898864997674167 1106 9 0.0594602490084597 1106 10-11 0.054657427544810844 1106 12-13 -0.025694014734114035 1106 14-15 0.05025843523493023 1106 16-17 0.025550564071309623 1106 18-19 0.19054802714065744 1106 20-21 0.16456800564510843 1106 22-23 0.35900671978398435 1106 24-25 0.1309859009172598 1106 26-27 0.1745492356384446 1106 28-29 0.05110867281486975 1106 30-31 0.18632297421157062 1106 32-33 0.07164296986742613 1106 34-35 0.14216299888779105 1106 36-37 0.06373979694027554 1106 38-39 0.23620904701424905 1106 40-41 0.4050268026029258 1106 42-43 0.4007532382659491 1106 44-45 0.17599891556604774 1106 46-47 0.25614471716152565 1106 48-49 -0.1752004220849841 1106 50-51 0.13957557023424982 1106 52-53 -0.041785584197270964 1106 54-55 -0.18989170495747487 1106 56-57 -0.37777720074492294 1106 58-59 -0.38635622300312633 1106 60-61 -0.2011429600390997 1106 62-63 -0.30322718645376057 1106 64-65 -0.4954152585862701 1106 66-67 -0.32287927175188713 1106 68-69 -0.3710369632698871 1106 70-71 0.012403321745029672 1106 72-73 -0.08637220919060695 1106 74-75 -0.13631236300875926 1106 76-77 -0.2242680016588139 1106 78-79 -0.1589886692675222 1106 80-81 -0.21674849360011805 1106 82-83 -0.1890204371254427 1106 84-85 0.047303507445892734 1106 86-87 0.1166984750347595 1106 88-89 -0.20515495567162745 1106 90-91 -0.13768764184277416 1106 92-93 0.016634282104803333 1106 94-95 0.10718278585989438 1106 96-97 0.6429180660354916 1106 98-99 0.7362725384690414 1106 100-101 0.9265252237828463 1107 1 -0.1140593349124508 1107 2 -0.08185739682101456 1107 3 -0.08468121937964312 1107 4 0.08856834608852893 1107 5 -0.039766517753662356 1107 6 -0.05641403231956588 1107 7 -0.14743400180115884 1107 8 -0.13375593966449628 1107 9 -0.11921560729896896 1107 10-11 -0.07186721740088586 1107 12-13 -0.050015234450071944 1107 14-15 -0.046180578967231156 1107 16-17 -0.05482746433303021 1107 18-19 -0.32342440393703953 1107 20-21 -0.07945872785530383 1107 22-23 -0.16377941221267633 1107 24-25 -0.21531735722810907 1107 26-27 -0.29121935249856534 1107 28-29 -0.28304963963291385 1107 30-31 -0.31486750197890245 1107 32-33 -0.24678852553775243 1107 34-35 -0.15151912308380133 1107 36-37 -0.3274256165935583 1107 38-39 -0.2507792570793157 1107 40-41 -0.2743069484413496 1107 42-43 -0.5194222003305455 1107 44-45 -0.391973097800701 1107 46-47 -0.5167876136655352 1107 48-49 -0.4312259024525744 1107 50-51 -0.6516942710355949 1107 52-53 -0.6457225098447239 1107 54-55 -0.7502091652749314 1107 56-57 -0.8740804588902904 1107 58-59 -0.574796072627187 1107 60-61 -0.5005686074910543 1107 62-63 -0.4983608539558446 1107 64-65 -0.7858914490624542 1107 66-67 -0.5109745098471308 1107 68-69 -0.24415726402176574 1107 70-71 -0.2537412062416031 1107 72-73 -0.30452174970857016 1107 74-75 -0.31381862867291943 1107 76-77 -0.5940988287264837 1107 78-79 -0.24240554478966203 1107 80-81 -0.23498157630688254 1107 82-83 -0.17853589243037504 1107 84-85 -0.10512965880306169 1107 86-87 -0.08512901034536213 1107 88-89 -0.5108358245103908 1107 90-91 -0.5152878924693383 1107 92-93 -0.2499383995994542 1107 94-95 -0.26732640378087424 1107 96-97 -0.3469002297038557 1107 98-99 -0.24206914908317145 1107 100-101 0.4554934777511015 1108 1 -0.04136477800736671 1108 2 -0.037155899487938626 1108 3 -0.1352435225735249 1108 4 -0.08235897220721 1108 5 0.018867246143258853 1108 6 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-1.4912098187333171 2316 16-17 -1.1926225693796013 2316 18-19 -0.781600708896967 2316 20-21 -1.2168350905728005 2316 22-23 -0.8665517752189587 2316 24-25 -0.9826482358650281 2316 26-27 -0.9959338135483122 2316 28-29 -1.0472182917158008 2316 30-31 -0.9305053620612114 2316 32-33 -0.9871827209027089 2316 34-35 -0.7682254980747842 2316 36-37 -0.984129103706401 2316 38-39 -0.7444836827525521 2316 40-41 -1.082291931475062 2316 42-43 -1.2969833872484529 2316 44-45 -1.5254291659546269 2316 46-47 -1.1481860686507375 2316 48-49 -1.242864051322691 2316 50-51 -1.1676064795403889 2316 52-53 -1.1864921293667763 2316 54-55 -1.111388863491669 2316 56-57 -1.0459380864984027 2316 58-59 -1.7702260054840195 2316 60-61 -1.1577052763236324 2316 62-63 -1.1853798417545676 2316 64-65 -0.8884977590761736 2316 66-67 -1.038546526308032 2316 68-69 -1.8063838180611853 2316 70-71 -1.6143505485273586 2316 72-73 -1.0864652916910984 2316 74-75 -1.255404073767135 2316 76-77 -1.1759557343650648 2316 78-79 -1.3265077756755232 2316 80-81 -1.2062399692027022 2316 82-83 -1.5399610602882916 2316 84-85 -1.554006996179421 2316 86-87 -1.3564668198897962 2316 88-89 -1.3581825864770352 2316 90-91 -1.3962928740591174 2316 92-93 -1.7146340894413434 2316 94-95 -1.9951222307969765 2316 96-97 -1.6261075071567497 2316 98-99 -2.2437441183994693 2316 100-101 -4.002942736240666 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 4.0 5 17.0 6 69.0 7 139.0 8 314.0 9 664.0 10 1150.0 11 1726.0 12 2648.0 13 3620.0 14 4856.0 15 6790.0 16 8802.0 17 11853.0 18 9815.0 19 5753.0 20 4821.0 21 4916.0 22 5307.0 23 5897.0 24 6799.0 25 8124.0 26 9890.0 27 12334.0 28 15080.0 29 18654.0 30 23097.0 31 28407.0 32 34673.0 33 43666.0 34 56438.0 35 74162.0 36 106879.0 37 157671.0 38 116240.0 39 7306.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.137246945308625 24.231380034166452 16.47504396050318 20.15632906002174 2 14.557691248002486 23.993853413684796 40.11511699553659 21.333338342776134 3 18.05213427424657 28.654940733737 32.89205587872488 20.40086911329155 4 11.535624797298228 20.677020787966747 41.124511167850216 26.66284324688481 5 10.420032107874444 40.72265258127775 36.840154378266824 12.017160932580977 6 25.621378760235263 42.01020051666256 18.47713524011786 13.891285482984323 7 23.71864305329288 36.15122710487398 23.995131400836726 16.13499844099641 8 23.550095355922398 35.83992727154337 21.625821607414096 18.984155765120146 9 24.267988334395625 18.74848951961402 24.43503337770337 32.54848876828699 10-11 22.137788368706225 28.99953292502883 29.50624039710138 19.356438309163558 12-13 24.25073442677145 27.911473086045014 29.214720592249765 18.62307189493377 14-15 21.629882855300313 29.48083172110671 28.70677510847311 20.182510315119867 16-17 18.192769349344122 32.06352724470048 30.276119117966555 19.46758428798884 18-19 17.572245649295155 30.906142484605436 33.40233101984466 18.119280846254746 20-21 18.74673642320749 29.335689160980582 33.26300077511905 18.654573640692874 22-23 18.694092624906634 29.30521615379173 33.024412508851576 18.97627871245006 24-25 19.255510044616305 30.130718381341044 32.92609321213594 17.687678361906716 26-27 18.744196675943638 29.099799955545674 34.38767300401654 17.768330364494144 28-29 17.599657383718963 29.771676803650564 34.48258805526441 18.14607775736606 30-31 18.661843080683617 29.457496844383 33.4487262326942 18.431933842239186 32-33 18.448687724439353 29.736104719057504 33.60389722203959 18.211310334463555 34-35 19.26192095149723 29.464620015953457 33.30603463928783 17.96742439326148 36-37 19.109645734120896 28.96243461840439 33.39529740877882 18.532622238695886 38-39 18.242221153653503 30.390970928252287 33.48397647813107 17.882831439963134 40-41 18.238600326952685 29.884176604538144 33.99080750801573 17.886415560493447 42-43 18.219546220718364 30.05893269950835 32.72449150823129 18.997029571541994 44-45 18.784064775746163 29.668388702062 32.44904488708261 19.098501635109237 46-47 19.777331433308373 30.44180589191396 31.42873779405099 18.352124880726684 48-49 19.212380914218166 30.350720775773116 31.488797671879226 18.94810063812949 50-51 18.61590360963002 30.53778804036134 31.81661085969451 19.029697490314128 52-53 19.07635437908652 31.460188044987042 30.956359713518466 18.50709786240797 54-55 19.027606985521548 30.475815399102892 31.035380920299637 19.461196695075923 56-57 19.466193084506486 30.88633214081972 30.41836500184419 19.2291097728296 58-59 19.569678997191108 30.117522538461778 31.057013931728576 19.255784532618534 60-61 19.373187793162618 30.771413004678077 31.38995873021837 18.465440471940934 62-63 19.056311348372653 30.331418194342252 31.74194154390469 18.8703289133804 64-65 19.0042208361514 31.04406202249443 30.901968888554897 19.04974825279928 66-67 18.93396797608694 30.775352368859178 30.099886107422176 20.190793547631706 68-69 18.664704015066754 30.45845779912958 31.020768752516187 19.85606943328748 70-71 19.500291452825028 30.553710278987033 30.869205614176565 19.076792654011374 72-73 19.247245447271244 29.53403949726204 31.56068155379439 19.658033501672328 74-75 18.73460535229716 31.338212035718715 30.263732933620346 19.663449678363783 76-77 18.827244170313318 30.60313926193722 30.798239588961927 19.771376978787536 78-79 18.832779798222678 30.30059962129182 30.801035436265817 20.065585144219693 80-81 19.140398239315566 30.58910537366951 30.160331496625602 20.110164890389314 82-83 19.078712534614503 30.74556915573802 30.52977254310609 19.64594576654138 84-85 18.625093712010234 30.51231737509919 30.305007932248017 20.55758098064256 86-87 18.25407361116503 30.773281669344627 30.567581681993456 20.405063037496884 88-89 19.059678353873377 30.359260245227766 29.948417607541618 20.632643793357243 90-91 18.85568455591345 29.657386909616644 30.79195528878499 20.69497324568492 92-93 19.278281815437456 30.29864805450371 30.61875337422698 19.804316755831856 94-95 18.96597185730865 29.60650712836697 30.637416238634792 20.790104775689585 96-97 18.871873449566756 29.6854405861452 30.3489633709363 21.093722593351742 98-99 18.66696318941539 30.196957927180694 29.6577668078282 21.478312075575715 100-101 18.2569356821283 29.963926057816042 30.00583899168382 21.773299268371836 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 785.0 1 538.5 2 295.5 3 289.0 4 258.0 5 284.0 6 444.0 7 732.0 8 1087.0 9 1346.0 10 1305.5 11 1146.0 12 1124.0 13 1147.5 14 1180.5 15 1232.0 16 1338.5 17 1473.5 18 1610.0 19 1712.0 20 1942.0 21 2307.5 22 2638.0 23 3057.5 24 3542.5 25 4489.0 26 6774.0 27 9603.0 28 10700.5 29 11967.0 30 14883.5 31 17963.5 32 22552.0 33 28539.5 34 34775.0 35 39306.5 36 45528.5 37 51114.0 38 49092.0 39 45271.0 40 40820.0 41 35318.0 42 31048.5 43 28429.5 44 27253.0 45 26770.5 46 24675.5 47 25303.5 48 35126.0 49 31902.5 50 17541.5 51 10876.0 52 7562.5 53 6052.0 54 5008.5 55 4193.5 56 3423.0 57 2834.0 58 2372.0 59 1976.0 60 1517.5 61 1113.0 62 805.5 63 519.0 64 312.0 65 201.0 66 141.5 67 113.5 68 79.5 69 56.0 70 48.0 71 36.5 72 25.0 73 23.5 74 24.0 75 21.5 76 19.5 77 15.0 78 13.5 79 10.5 80 7.0 81 6.0 82 5.0 83 2.5 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017906627580964878 2 0.011896011330011632 3 0.00901592437642987 4 0.0 5 0.0036314139849509194 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 6.261058594742965E-4 14-15 2.504423437897186E-4 16-17 6.261058594742965E-5 18-19 0.0011896011330011635 20-21 0.0 22-23 6.261058594742965E-5 24-25 0.0 26-27 0.0018157069924754597 28-29 0.0032557504692663415 30-31 0.0013774328908434522 32-33 0.008702871446692722 34-35 0.00175309640652803 36-37 7.513270313691557E-4 38-39 0.0013774328908434522 40-41 6.261058594742965E-5 42-43 0.006135837422848105 44-45 0.0020661493362651782 46-47 1.252211718948593E-4 48-49 3.7566351568457787E-4 50-51 1.252211718948593E-4 52-53 1.8783175784228893E-4 54-55 0.002629644609792045 56-57 0.016717026447963714 58-59 0.028738258949870206 60-61 0.022790253284864392 62-63 0.01559003590090998 64-65 0.020911935706441502 66-67 0.0034435822271086307 68-69 9.391587892114447E-4 70-71 1.8783175784228893E-4 72-73 0.0 74-75 0.00175309640652803 76-77 0.0029426975395291932 78-79 0.010393357267273322 80-81 0.018470122854491747 82-83 0.018783175784228893 84-85 0.032995778794295424 86-87 0.011770790158116773 88-89 0.04376479957725332 90-91 0.049838026414154 92-93 0.03205662000508398 94-95 0.03287055762240057 96-97 0.052968555711525485 98-99 0.055973863837002105 100-101 0.06311147063500909 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 798587.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.24347320994703 #Duplication Level Percentage of deduplicated Percentage of total 1 73.75573970856917 21.56873998247369 2 12.596874646906475 7.367527325317409 3 4.5844536419245125 4.021960417795907 4 2.1933796438346302 2.565681554148847 5 1.2761550260481258 1.8659602657988814 6 0.8469891140393901 1.4861342079316606 7 0.5998981835555338 1.2280174521651497 8 0.4230787085379216 0.9897832703062159 9 0.33401753477987245 0.8791049546988975 >10 2.498757630110787 15.275481602865456 >50 0.4691275112917304 9.66468917246089 >100 0.38550789085584575 22.03378585144123 >500 0.027015569659530905 5.217201612713059 >1k 0.007718734188437402 3.799914509865184 >5k 0.0012864556980729002 2.036017820017528 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5793 0.72540624878692 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5396 0.6756934435446608 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5047 0.6319912545533548 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2897 0.3627657349794074 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2726 0.3413529145853864 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2423 0.30341089950124406 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1881 0.23554102433423035 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1755 0.21976315667547805 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1735 0.21725873323758088 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1699 0.21275077104936593 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1630 0.20411051018862067 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1598 0.20010343268798514 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1561 0.19547024932787538 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1555 0.1947189222965062 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1498 0.18758131549849924 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1436 0.17981760284101794 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1402 0.17556008299659273 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1245 0.15590035900909982 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1145 0.1433782418196139 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1092 0.13674151970918635 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1024 0.12822648002033593 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 933 0.11683135337790373 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 921 0.11532869931516541 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 888 0.11119640064263506 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 874 0.10944330423610703 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 872 0.10919286189231729 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 854 0.10693888079820985 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 851 0.10656321728252525 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 835 0.10455967853220752 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 835 0.10455967853220752 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 833 0.10430923618841781 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 831 0.10405879384462807 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 817 0.10230569743810004 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 800 0.10017693751588745 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.504423437897186E-4 0.0 14-15 0.0 0.0 0.0 2.504423437897186E-4 0.0 16-17 0.0 0.0 0.0 2.504423437897186E-4 0.0 18-19 0.0 0.0 0.0 2.504423437897186E-4 0.0 20-21 0.0 0.0 0.0 3.1305292973714824E-4 0.0 22-23 0.0 0.0 0.0 7.513270313691558E-4 0.0 24-25 0.0 0.0 0.0 0.0013148223048960227 0.0 26-27 0.0 0.0 0.0 0.00175309640652803 0.0 28-29 0.0 0.0 0.0 0.004132298672530357 0.0 30-31 0.0 0.0 0.0 0.009704640821851594 0.0 32-33 0.0 0.0 0.0 0.0192214498858609 0.0 34-35 0.0 0.0 0.0 0.03355927406782229 0.0 36-37 0.0 0.0 0.0 0.04695793946057224 0.0 38-39 0.0 0.0 0.0 0.060231383681427324 0.0 40-41 0.0 0.0 0.0 0.0720021738395441 0.0 42-43 0.0 0.0 0.0 0.08602694509176834 0.0 44-45 0.0 0.0 0.0 0.09836123052341197 0.0 46-47 0.0 0.0 0.0 0.11708179572169344 0.0 48-49 0.0 0.0 0.0 0.14124948189740127 0.0 50-51 0.0 0.0 0.0 0.15959438357999817 0.0 52-53 0.0 0.0 0.0 0.19190144592887187 0.0 54-55 0.0 0.0 0.0 0.2557642435952501 0.0 56-57 0.0 0.0 0.0 0.36489449490161996 0.0 58-59 0.0 0.0 0.0 0.4741499673798847 0.0 60-61 0.0 0.0 0.0 0.5816523434516214 0.0 62-63 0.0 0.0 0.0 0.7022403319863709 0.0 64-65 0.0 0.0 0.0 0.8555736569716261 0.0 66-67 0.0 0.0 0.0 1.0843527380235343 0.0 68-69 0.0 0.0 0.0 1.3457519343540527 0.0 70-71 0.0 0.0 0.0 1.5729031401713276 0.0 72-73 0.0 0.0 0.0 1.8028718223562366 0.0 74-75 0.0 0.0 0.0 2.072911279547501 0.0 76-77 0.0 0.0 0.0 2.377950054283378 0.0 78-79 0.0 0.0 0.0 2.627014965182253 0.0 80-81 0.0 0.0 0.0 2.879961732409869 0.0 82-83 0.0 0.0 0.0 3.1600188833527216 0.0 84-85 0.0 0.0 0.0 3.591844094632144 0.0 86-87 0.0 0.0 0.0 4.137683182921836 0.0 88-89 0.0 0.0 0.0 4.749012944112538 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGCG 80 4.4941425E-4 29.685562 5 GGACAAT 405 0.0 26.97017 6 ATGGGTA 370 0.0 26.9577 5 GGATATG 365 0.0 26.03385 1 ACAGTGC 110 9.485354E-5 25.904156 8 GAGTACT 4550 0.0 24.476109 12-13 CATATAG 280 1.8189894E-12 23.748451 3 GACAATG 465 0.0 23.490149 7 GTACATG 3810 0.0 23.194725 1 GTACTTT 4785 0.0 23.07554 14-15 GACATAT 290 3.6379788E-12 22.93672 1 CATTCCG 235 4.654794E-9 22.229807 9 ATGGGAT 775 0.0 22.063076 5 ACATGGG 3830 0.0 21.950264 3 CGTCTTA 65 2.9459957E-4 21.918901 14-15 GGCATTC 240 5.9335434E-9 21.766687 7 ACTATAT 375 0.0 21.529232 8 GGATGAC 355 0.0 21.404373 8 GATCCTC 200 5.391721E-7 21.370928 6 CATGGGT 560 0.0 21.203976 4 >>END_MODULE