FastQCFastQC Report
Thu 26 May 2016
SRR936643_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936643_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960316
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38290.39872292037204415No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT37880.3944534923920876No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33520.34905177045889063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26990.2810533199488502No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25860.26928635990653077No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC24790.2581441942027416No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT16780.1747341500089554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15090.15713577614035382No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC12400.12912416329624832No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12000.12495886770604676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG31750.023.7902971
TACATGG31150.023.6385122
GAGTACT34550.023.3747712-13
ACATGGG31950.022.45073
GTGTAAG4100.022.0148741
GTACTTT37050.021.54108214-15
GTGTAGC6050.021.2009281
AGAGTAC49800.020.22387110-11
GCACCGT2351.1305565E-720.2141726
ACAACGC1200.00476245419.7920158
CATGGGG18150.019.6304874
TATACTG4850.019.5889935
AGTACTT36600.019.40468412-13
ATAAGAC3455.2750693E-1119.2767053
GTATAGC3003.7071004E-919.0023121
TAAGGCG3759.094947E-1219.0013245
CCTATAC2552.6717134E-718.6287483
ACACCGT1851.5201957E-417.9742246
GTATCAA82300.017.8940371
ATGGGGG9850.017.8438845
Produced by FastQC (version 0.11.5)