##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936643_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 960316 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.477034642763424 31.0 31.0 34.0 30.0 34.0 2 31.55155594616772 33.0 31.0 34.0 30.0 34.0 3 31.575689668817347 33.0 31.0 34.0 28.0 34.0 4 35.192586606908556 37.0 35.0 37.0 33.0 37.0 5 35.200677693592525 37.0 35.0 37.0 33.0 37.0 6 35.1643000845555 37.0 35.0 37.0 32.0 37.0 7 35.0775380187355 37.0 35.0 37.0 32.0 37.0 8 35.04185289009035 37.0 35.0 37.0 32.0 37.0 9 36.56264396302883 39.0 37.0 39.0 32.0 39.0 10-11 36.445573123846735 39.0 37.0 39.0 32.0 39.0 12-13 36.360640143452784 39.0 37.0 39.0 32.0 39.0 14-15 37.49478661190692 40.0 37.0 41.0 32.0 41.0 16-17 37.41618904610566 40.0 37.0 41.0 32.0 41.0 18-19 37.39382557408186 40.0 37.0 41.0 32.0 41.0 20-21 37.248186013770464 40.0 37.0 41.0 31.0 41.0 22-23 37.33965590493129 40.0 37.0 41.0 32.0 41.0 24-25 37.38163583653714 40.0 37.0 41.0 32.0 41.0 26-27 37.28530400409865 40.0 37.0 41.0 31.0 41.0 28-29 37.15361922533833 39.5 36.5 41.0 31.0 41.0 30-31 36.94539818143194 39.0 36.0 41.0 30.5 41.0 32-33 36.72852165328912 39.0 36.0 41.0 30.0 41.0 34-35 36.52304085321915 39.0 35.0 41.0 30.0 41.0 36-37 36.31525508270194 39.0 35.0 40.5 30.0 41.0 38-39 36.094051333102854 39.0 35.0 40.0 29.5 41.0 40-41 35.88154159672441 38.5 35.0 40.0 28.5 41.0 42-43 35.64699536402601 38.0 35.0 40.0 27.5 41.0 44-45 35.36940184272677 38.0 35.0 40.0 26.5 41.0 46-47 35.20247762194944 38.0 35.0 40.0 26.0 41.0 48-49 35.03687744450785 38.0 34.0 40.0 25.0 41.0 50-51 34.055677506154225 37.5 33.0 39.5 23.5 40.5 52-53 34.16292345436294 38.0 33.5 39.5 24.0 40.5 54-55 34.60039611961063 38.0 34.0 40.0 24.0 41.0 56-57 34.458068489955394 38.0 34.0 40.0 23.5 41.0 58-59 34.217577339125874 38.0 34.0 40.0 23.0 41.0 60-61 33.91232000716431 37.5 33.0 40.0 22.0 41.0 62-63 33.57455306378317 37.0 33.0 40.0 22.0 41.0 64-65 33.214998500493586 36.5 33.0 39.5 20.5 41.0 66-67 32.70569375080703 36.0 32.0 39.0 18.5 41.0 68-69 32.32368355832872 35.5 31.5 39.0 18.0 40.5 70-71 31.914519803897882 35.0 31.0 38.5 15.5 40.0 72-73 31.529903177704007 35.0 31.0 37.5 12.5 40.0 74-75 31.11292272543621 35.0 31.0 37.0 9.0 39.0 76-77 30.661126649977717 35.0 30.0 36.5 7.0 39.0 78-79 30.16458124200784 34.0 30.0 36.0 4.5 38.5 80-81 29.707123488518363 34.0 29.0 35.5 2.0 37.0 82-83 29.295750565438876 34.0 29.0 35.0 2.0 37.0 84-85 28.94239604463531 34.0 29.0 35.0 2.0 36.5 86-87 28.62747002028499 34.0 29.0 35.0 2.0 36.0 88-89 28.21674844530342 34.0 28.0 35.0 2.0 36.0 90-91 27.813674353025462 34.0 27.0 35.0 2.0 35.0 92-93 27.354659820309145 33.5 26.0 35.0 2.0 35.0 94-95 26.854239646116486 33.0 25.0 35.0 2.0 35.0 96-97 26.284952036621277 33.0 24.5 35.0 2.0 35.0 98-99 25.560725323747597 33.0 22.0 35.0 2.0 35.0 100-101 22.871959334219152 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.46300463800241687 1101 2 0.4850374876029484 1101 3 0.3781661611062006 1101 4 0.5293189348375762 1101 5 0.3204858459920743 1101 6 0.5051802083730621 1101 7 0.48770593021452413 1101 8 0.44563399025275174 1101 9 0.4571601828947891 1101 10-11 0.6859114235924082 1101 12-13 0.7714961584865137 1101 14-15 0.7927720020303681 1101 16-17 0.7134187680269903 1101 18-19 0.7412715280804818 1101 20-21 0.7299814470401458 1101 22-23 0.5852920696052237 1101 24-25 0.7052551989011988 1101 26-27 0.6392904894038338 1101 28-29 0.6271340697639189 1101 30-31 0.5280165571494777 1101 32-33 0.4194476293660472 1101 34-35 0.402028889262823 1101 36-37 0.4895048545935907 1101 38-39 0.500956736955807 1101 40-41 0.4785338366455818 1101 42-43 0.5975043751639149 1101 44-45 0.5246793370811176 1101 46-47 0.7677096811370419 1101 48-49 1.00451256721756 1101 50-51 0.8935814138988576 1101 52-53 1.0104883312865809 1101 54-55 0.7771173879326057 1101 56-57 0.7940572516465068 1101 58-59 0.8175494264857122 1101 60-61 0.9414618495923648 1101 62-63 0.9587357271498789 1101 64-65 0.7646795239184598 1101 66-67 0.7875664771304898 1101 68-69 0.8302453748795102 1101 70-71 0.817606104303298 1101 72-73 0.629496813982545 1101 74-75 0.5785647651702313 1101 76-77 0.5413889216240904 1101 78-79 0.6422404457871842 1101 80-81 0.6596369388930405 1101 82-83 0.6804284752142102 1101 84-85 0.7102765388295609 1101 86-87 0.7496147502015482 1101 88-89 0.7775197148724011 1101 90-91 0.8324508239962221 1101 92-93 0.8282400857333414 1101 94-95 0.7613460622319614 1101 96-97 0.8250721771406404 1101 98-99 0.9971657866634551 1101 100-101 1.0375563570388273 1102 1 0.29932097515121825 1102 2 0.41711683056607995 1102 3 0.30361693331265016 1102 4 0.39494972197667977 1102 5 0.366491948392472 1102 6 0.49553277191033374 1102 7 0.44225598106785924 1102 8 0.41034177463460253 1102 9 0.4341215637539477 1102 10-11 0.49638074403898 1102 12-13 0.4508106084768144 1102 14-15 0.6246126576562716 1102 16-17 0.36265920142675867 1102 18-19 0.3967886741794686 1102 20-21 0.2693078313973132 1102 22-23 0.25157777717208063 1102 24-25 0.1797024260715716 1102 26-27 -0.003590925318356142 1102 28-29 -0.011222279848361438 1102 30-31 0.2565479245457283 1102 32-33 0.3333414664119658 1102 34-35 0.256961075015667 1102 36-37 0.11409611722609014 1102 38-39 0.16593495526653612 1102 40-41 0.23944555172580095 1102 42-43 0.12006032853170012 1102 44-45 0.16835294684496205 1102 46-47 0.3501999660920916 1102 48-49 0.4761049527387655 1102 50-51 0.5606199272177221 1102 52-53 0.713655347753658 1102 54-55 0.5955536331338749 1102 56-57 0.4740433772101582 1102 58-59 0.5683654314711006 1102 60-61 0.5382857206776848 1102 62-63 0.4870075367643949 1102 64-65 0.6078739953083172 1102 66-67 0.46290819391561655 1102 68-69 0.5494073824399024 1102 70-71 0.5744095752639886 1102 72-73 -1.888947601145219E-4 1102 74-75 0.1830304523957551 1102 76-77 0.2726108126627338 1102 78-79 0.12120274377009821 1102 80-81 0.34008103843624227 1102 82-83 0.39947466650506414 1102 84-85 0.006579530651876553 1102 86-87 0.11293597279806633 1102 88-89 0.017688265758074806 1102 90-91 0.022475045469853683 1102 92-93 0.031160772165200967 1102 94-95 -0.09283655121801004 1102 96-97 -0.06297493715296909 1102 98-99 -0.0605171824659223 1102 100-101 0.01280195219923641 1103 1 0.2626286218613565 1103 2 0.15838086694932585 1103 3 0.2959166396077144 1103 4 0.3352931256719316 1103 5 0.34105817265979965 1103 6 0.41073175114335214 1103 7 0.49740117579389675 1103 8 0.4585391898972091 1103 9 0.204301972931475 1103 10-11 0.42770904721481173 1103 12-13 0.07971358537894702 1103 14-15 0.4129016338417202 1103 16-17 0.6010426186518671 1103 18-19 0.5477067951376782 1103 20-21 0.616422691125571 1103 22-23 0.32379369216175036 1103 24-25 0.31063856450472827 1103 26-27 0.13719035660154333 1103 28-29 0.25328714583625356 1103 30-31 0.22909220634171845 1103 32-33 0.26606160278215185 1103 34-35 0.4438855121370153 1103 36-37 0.5281507523722198 1103 38-39 0.4472944038916111 1103 40-41 0.27664644657139803 1103 42-43 0.5661857341760665 1103 44-45 0.6627613340130338 1103 46-47 0.9127715085285999 1103 48-49 0.6092194306841066 1103 50-51 0.6638804873669528 1103 52-53 0.8516666940743036 1103 54-55 1.0140845056418186 1103 56-57 0.9052723833354293 1103 58-59 0.7280625503495983 1103 60-61 0.9724511063621648 1103 62-63 1.066889251036713 1103 64-65 0.8072778781076551 1103 66-67 0.9224929547487122 1103 68-69 0.8254503363680783 1103 70-71 0.742552301796902 1103 72-73 0.5488636807676031 1103 74-75 0.6670412829745587 1103 76-77 0.7249296415938424 1103 78-79 0.6900162696024026 1103 80-81 0.7184681060419607 1103 82-83 0.4358229128153326 1103 84-85 0.499234395537556 1103 86-87 0.42087002482101354 1103 88-89 0.49406578209269014 1103 90-91 0.5718904822261699 1103 92-93 0.5109408451058357 1103 94-95 0.4554298528891678 1103 96-97 0.42619883770897005 1103 98-99 0.28553102934992225 1103 100-101 0.12021205739047858 1104 1 0.4472499692340577 1104 2 0.5276607412403678 1104 3 0.3726036614910342 1104 4 0.3877688975859428 1104 5 0.42190263939638584 1104 6 0.40153016434840794 1104 7 0.5112828255836206 1104 8 0.511017066313805 1104 9 0.5297054236142102 1104 10-11 0.7231082538173368 1104 12-13 0.5942215000624103 1104 14-15 0.5425814337736838 1104 16-17 0.4385359105498807 1104 18-19 0.4761421325420514 1104 20-21 0.4582942252728799 1104 22-23 0.37513776781160146 1104 24-25 0.43356028051613293 1104 26-27 0.16205277474517032 1104 28-29 0.21134984598322148 1104 30-31 0.3902406334014117 1104 32-33 0.37630439223017476 1104 34-35 0.541461125161085 1104 36-37 0.37535877894507763 1104 38-39 0.37669114822611505 1104 40-41 0.21533228993327214 1104 42-43 0.33882474534045315 1104 44-45 0.4774593554298576 1104 46-47 0.3918992272828774 1104 48-49 0.8457251847449143 1104 50-51 0.7466828430068446 1104 52-53 0.8094314317305162 1104 54-55 0.743867075449927 1104 56-57 0.5532346987091685 1104 58-59 0.6690407231503102 1104 60-61 0.9410871782379431 1104 62-63 1.0981063901057482 1104 64-65 0.9463572634526045 1104 66-67 1.0122563034332188 1104 68-69 0.8865233520852058 1104 70-71 0.6793539138458762 1104 72-73 0.6884732253883179 1104 74-75 0.6485655040455391 1104 76-77 0.5130681389088494 1104 78-79 0.7441910766019788 1104 80-81 0.7914399793649345 1104 82-83 0.9091778056084614 1104 84-85 0.9630399324838521 1104 86-87 1.0688230453083456 1104 88-89 0.9994085880259824 1104 90-91 0.657939559226417 1104 92-93 0.3268984113187443 1104 94-95 0.49330826875992173 1104 96-97 0.530258773942542 1104 98-99 0.6595025413999558 1104 100-101 0.4788785744036268 1105 1 0.3177675770929653 1105 2 0.28567352755786146 1105 3 0.3977979593515002 1105 4 0.37029234874270145 1105 5 0.37650628817478804 1105 6 0.3466811298877204 1105 7 0.21152574920220246 1105 8 0.3719777635702428 1105 9 0.39979463238900337 1105 10-11 0.4931178558657763 1105 12-13 0.35826141832189506 1105 14-15 0.44146817063651866 1105 16-17 0.4285744008124155 1105 18-19 0.25721871093005433 1105 20-21 0.3422875284596145 1105 22-23 0.032297038712478354 1105 24-25 0.1809028930939931 1105 26-27 0.03437384426563739 1105 28-29 0.09266278248659887 1105 30-31 0.060228076876533976 1105 32-33 -0.034453184770868006 1105 34-35 0.0836953659016757 1105 36-37 0.15413702086745218 1105 38-39 0.16280018482058978 1105 40-41 0.02413293663533267 1105 42-43 0.14392901373822298 1105 44-45 0.2558691084848661 1105 46-47 0.4422833092203575 1105 48-49 0.48480716325930473 1105 50-51 0.37901794629041774 1105 52-53 0.4315350189573337 1105 54-55 0.48796961219622403 1105 56-57 0.2736735205296199 1105 58-59 0.2700152900322692 1105 60-61 0.2756721974010077 1105 62-63 0.25413926686304933 1105 64-65 0.26050961595446864 1105 66-67 0.39139852043951606 1105 68-69 0.41391955558297866 1105 70-71 0.43402075559262343 1105 72-73 0.20112166731233927 1105 74-75 0.3295230826879596 1105 76-77 0.25550180680928136 1105 78-79 0.2828089561085818 1105 80-81 0.3688370035506061 1105 82-83 0.28011478498457976 1105 84-85 0.44521623265865884 1105 86-87 0.19226435381313323 1105 88-89 0.1050947241452782 1105 90-91 0.16109059042075913 1105 92-93 0.013661536169994548 1105 94-95 0.25026154699774494 1105 96-97 0.13239010042039823 1105 98-99 0.3540921564152626 1105 100-101 0.390815069573879 1106 1 0.2550455548149486 1106 2 0.2871909689728511 1106 3 0.1816205791671841 1106 4 0.3041706580475676 1106 5 0.21410563256014825 1106 6 0.27414210005081685 1106 7 0.4300206560847144 1106 8 0.4334061018025466 1106 9 0.5341278965037617 1106 10-11 0.5699826764323532 1106 12-13 0.6436184140433028 1106 14-15 0.5508569299102319 1106 16-17 0.4540491495434438 1106 18-19 0.22534078140196812 1106 20-21 0.054467258373918526 1106 22-23 0.25763098131731965 1106 24-25 0.12512678816011658 1106 26-27 0.28646733597994967 1106 28-29 0.26658527693152223 1106 30-31 0.38500662370844907 1106 32-33 0.18655330141669424 1106 34-35 0.292719173019492 1106 36-37 0.23623043484968065 1106 38-39 0.32091113960383666 1106 40-41 0.516162066180307 1106 42-43 0.4032957304125304 1106 44-45 0.2763803857452345 1106 46-47 0.18661192051438746 1106 48-49 0.35627326818207905 1106 50-51 -0.13808366842715714 1106 52-53 0.1940435000834242 1106 54-55 0.16776643162125282 1106 56-57 0.18411810365797976 1106 58-59 0.3714137458288036 1106 60-61 0.42460132683492446 1106 62-63 0.4027444200563366 1106 64-65 0.3673679181301921 1106 66-67 0.5007886677862459 1106 68-69 0.6924191930183099 1106 70-71 0.3214716316887909 1106 72-73 0.061866851531835465 1106 74-75 0.7120482428993462 1106 76-77 0.7278977540802742 1106 78-79 0.3052349412795188 1106 80-81 0.6039124425425761 1106 82-83 0.5962822820890565 1106 84-85 0.62903568766788 1106 86-87 0.8024326548895004 1106 88-89 0.6103316459989898 1106 90-91 0.462403485396937 1106 92-93 0.5610850505709735 1106 94-95 0.6146609722244705 1106 96-97 0.8099068711185069 1106 98-99 0.882223676888227 1106 100-101 0.5314399134156673 1107 1 0.23179270374572525 1107 2 0.4070988500434609 1107 3 0.29837246209517687 1107 4 0.30456063076887574 1107 5 0.3923263998039914 1107 6 0.33244851901473993 1107 7 0.535760175001947 1107 8 0.574388031126297 1107 9 0.5553002463383976 1107 10-11 0.6539147655608488 1107 12-13 0.5222955075759685 1107 14-15 0.2912460101566623 1107 16-17 0.40804724301013806 1107 18-19 0.35451390988860254 1107 20-21 0.4103002284656583 1107 22-23 0.3978653012449911 1107 24-25 0.30276906353130073 1107 26-27 0.4413498849917019 1107 28-29 0.30362169120026294 1107 30-31 0.3949409039274556 1107 32-33 0.06659174250882671 1107 34-35 0.21990842037860148 1107 36-37 0.32986786498765497 1107 38-39 0.16335698642669172 1107 40-41 0.5085514542658913 1107 42-43 0.31693746771190234 1107 44-45 0.28850025935009427 1107 46-47 0.5587315871685448 1107 48-49 0.5311550152598699 1107 50-51 0.5803437390885193 1107 52-53 0.5813488209152098 1107 54-55 0.7327673823010201 1107 56-57 0.6257127363916339 1107 58-59 0.42578810153307955 1107 60-61 0.5682406324635991 1107 62-63 0.7846121113014135 1107 64-65 0.6385034048717628 1107 66-67 0.6963683948958774 1107 68-69 0.6918167724712063 1107 70-71 0.4132762547086877 1107 72-73 0.39630079610267543 1107 74-75 0.5273282536607802 1107 76-77 0.5211404257620487 1107 78-79 0.45095183789859306 1107 80-81 0.6840612142255118 1107 82-83 0.6353581308132696 1107 84-85 0.36076388120817526 1107 86-87 0.5780505644028935 1107 88-89 0.32089007658836977 1107 90-91 0.2531236220878732 1107 92-93 0.5986466022355685 1107 94-95 0.6441695548579425 1107 96-97 0.7773781017378987 1107 98-99 0.9911521767155556 1107 100-101 0.9499805617663348 1108 1 0.010488487841573857 1108 2 -0.029861861108472 1108 3 0.04754133607728406 1108 4 1.9793672329626588E-4 1108 5 -0.111482756933178 1108 6 -0.25540072202410613 1108 7 -0.1509614900382772 1108 8 -0.1098882290936416 1108 9 -0.11148341488036806 1108 10-11 0.03677479032651121 1108 12-13 -0.19521021230955427 1108 14-15 0.0858217545618345 1108 16-17 0.07404037094696747 1108 18-19 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2316 48-49 -2.244054266573002 2316 50-51 -1.7492773506456558 2316 52-53 -1.984500789894014 2316 54-55 -1.8700678357926819 2316 56-57 -1.979896016729235 2316 58-59 -1.7431728325247846 2316 60-61 -2.131804161191859 2316 62-63 -1.8824557400717659 2316 64-65 -1.9113350442397063 2316 66-67 -2.0376388014618847 2316 68-69 -1.8893265672262558 2316 70-71 -1.6948123045511103 2316 72-73 -1.7744261828957058 2316 74-75 -1.6494656955241211 2316 76-77 -1.8988683861045992 2316 78-79 -2.624539423666981 2316 80-81 -2.2478311341369484 2316 82-83 -2.146981419781529 2316 84-85 -1.9481919125941474 2316 86-87 -1.6816557067088667 2316 88-89 -2.157971723969716 2316 90-91 -2.4240991712738555 2316 92-93 -2.9758423094989013 2316 94-95 -2.9177595096155464 2316 96-97 -2.995946174954714 2316 98-99 -4.094002030950772 2316 100-101 -5.070680328232196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2714.0 3 989.0 4 907.0 5 965.0 6 1064.0 7 1219.0 8 1589.0 9 2071.0 10 2505.0 11 3256.0 12 3947.0 13 4782.0 14 5669.0 15 6669.0 16 7906.0 17 9965.0 18 8321.0 19 6433.0 20 6467.0 21 6847.0 22 7460.0 23 8507.0 24 9710.0 25 10999.0 26 12742.0 27 14765.0 28 17634.0 29 21071.0 30 25625.0 31 31635.0 32 39554.0 33 50948.0 34 68048.0 35 92887.0 36 132061.0 37 173469.0 38 137587.0 39 21321.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.81191762888842 17.888940846144152 14.583348521283787 22.715793003683643 2 15.532942164012006 19.352571032224603 40.42280862905359 24.6916781747098 3 20.168048848309255 23.279389541937746 30.00790462790448 26.54465698184852 4 12.787829803898696 15.774448087522758 37.75825004478626 33.67947206379229 5 13.712330336774341 36.43444202543776 34.99005197970812 14.863175658079772 6 32.342054296879475 35.188374271126015 17.401192451902393 15.068378980092117 7 28.418477649616975 32.087410388106456 20.983450177661698 18.51066178461487 8 26.190996981495253 33.37457998204312 20.167840529623636 20.266582506837985 9 25.46531443881016 17.11496714569644 21.08217117571323 36.337547239780164 10-11 25.192776481373446 25.416857654804563 27.582298524877075 21.808067338944912 12-13 26.44602616894082 23.08120996715601 27.883015813656975 22.5897480502462 14-15 22.96090208882248 24.657448633570425 26.894286010404787 25.487363267202305 16-17 21.922342171927603 27.95771390953635 27.529655261491282 22.590288657044766 18-19 21.82982184966378 26.680366799710026 29.77336452491855 21.716446825707646 20-21 22.595224204034007 25.36813427237873 29.732715869311555 22.303925654275712 22-23 22.087387867303416 25.241761921202094 30.076948272243893 22.593901939250596 24-25 22.53083977065166 25.350260234971667 29.94748439527391 22.17141559910277 26-27 22.041110875653633 25.35672241802218 30.453069361781225 22.149097344542962 28-29 21.706969372581526 26.297073046788768 30.01938945097239 21.97656812965732 30-31 21.91346020172105 25.466202013669882 29.855308806278124 22.765028978330946 32-33 21.81744245742688 25.6172881288862 30.0422060442221 22.52306336946482 34-35 22.81135730913434 25.51239670712386 29.565127294102822 22.111118689638978 36-37 22.431748815230034 25.15752676471109 30.17911868317934 22.23160573687954 38-39 22.079086467371155 25.79322847895901 29.860743755180586 22.266941298489247 40-41 22.163802704480027 25.94998102146683 29.79515159656585 22.091064677487292 42-43 22.002644032884312 25.953593526024566 29.10065913375901 22.943103307332112 44-45 22.19213873301881 25.642657091786287 29.313108897776246 22.852095277418655 46-47 22.898378225274026 26.050758820625923 28.959310886321653 22.091552067778395 48-49 22.623054270088346 26.04430037029786 28.750203059851632 22.58244229976216 50-51 22.03272809067393 26.50810808559206 29.165260820847337 22.29390300288667 52-53 22.607285299232522 26.598735798560853 28.29082881212967 22.503150090076957 54-55 22.01242371857622 26.200560264928246 28.766740257846834 23.0202757586487 56-57 22.49536347154926 26.224944262732098 28.364255671872286 22.915436593846355 58-59 22.934069659005054 26.024263131942433 28.324933729127473 22.71673347992504 60-61 22.356000279080327 26.143422295394032 29.290209174869858 22.210368250655787 62-63 22.06392908986019 26.37517520311065 29.10584454667952 22.455051160349633 64-65 21.82077757223062 26.76548550233024 28.764372319549064 22.649364605890078 66-67 22.03501306037769 26.34853086373464 28.318158715875335 23.29829736001233 68-69 22.03594380082984 26.198586791090722 28.736289550525395 23.02917985755404 70-71 22.567758544080103 26.074622301886574 28.275619217584218 23.081999936449098 72-73 22.21777282268576 25.96899850496704 28.92802856132629 22.885200111020907 74-75 21.95623824294681 26.942374554976656 28.32650391328893 22.7748832887876 76-77 22.080965676065425 26.403290998032965 28.28533440408469 23.230408921816924 78-79 22.152341202284628 26.50014690468699 28.35541765731825 22.992094235710134 80-81 22.63974567867912 26.322475558151616 27.988997195108034 23.048781568061226 82-83 22.36091366882865 26.231748996842718 28.567491921868594 22.83984541246004 84-85 22.251332484501 26.502576440476282 28.27028279656405 22.97580827845867 86-87 22.2877809862804 26.644464638626843 28.146148017570678 22.921606357522087 88-89 22.031447978764568 26.519031740137812 27.745891415783987 23.70362886531364 90-91 22.048427277571147 26.19129342363425 28.174469035928524 23.585810262866083 92-93 22.366385220729267 26.249932949797493 28.480478366089418 22.90320346338382 94-95 22.09307048449433 26.15614295816379 28.4669708403588 23.283815716983078 96-97 21.608029733427138 26.232448367749626 28.875263440476644 23.284258458346592 98-99 21.881894271727877 26.660238307705853 27.88308513303942 23.574782287526848 100-101 21.935837503360684 26.215108802804426 28.040299033165006 23.808754660669887 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 95.0 1 77.5 2 89.5 3 122.5 4 116.5 5 124.0 6 201.0 7 391.0 8 635.5 9 790.0 10 764.5 11 718.0 12 762.5 13 806.0 14 913.5 15 1033.0 16 1100.5 17 1203.5 18 1315.5 19 1455.5 20 1682.5 21 1960.5 22 2304.0 23 2798.0 24 3411.0 25 4194.5 26 5391.5 27 6905.5 28 8191.5 29 9648.5 30 11556.5 31 13614.5 32 16095.0 33 17619.5 34 18635.5 35 20169.5 36 21921.5 37 23617.5 38 24906.0 39 25761.0 40 26481.5 41 28014.0 42 29011.5 43 29591.0 44 30776.5 45 32443.5 46 34371.0 47 38415.0 48 45631.5 49 44869.0 50 37167.5 51 33399.5 52 32330.0 53 32040.5 54 31427.5 55 29420.5 56 26859.0 57 24340.5 58 22545.0 59 20057.5 60 16447.5 61 13758.0 62 11614.5 63 9078.0 64 6389.0 65 4705.0 66 3561.0 67 2801.0 68 2292.0 69 1796.0 70 1388.0 71 1046.5 72 795.0 73 629.5 74 501.0 75 373.0 76 274.0 77 205.0 78 130.0 79 68.5 80 49.5 81 46.5 82 34.0 83 20.5 84 15.0 85 13.0 86 11.5 87 5.5 88 4.0 89 4.0 90 4.5 91 2.0 92 1.0 93 2.0 94 2.0 95 2.0 96 1.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013016548719379871 2 0.012287621991094599 3 0.012391754380849636 4 0.020826477951007793 5 0.0339471590601427 6 0.046338913440992335 7 0.006664472944322493 8 0.024783508761699272 9 0.033322364721612464 10-11 0.04123642634299543 12-13 0.033634761890877586 14-15 0.028896738157023316 16-17 0.029573598690431066 18-19 0.023742184864148884 20-21 0.049567017523398545 22-23 0.01306861491425739 24-25 0.003123971692651169 26-27 0.0010933900924279092 28-29 0.0 30-31 0.0030719054977736498 32-33 0.0028115745233860522 34-35 5.2066194877519476E-5 36-37 3.123971692651169E-4 38-39 0.0 40-41 0.002134713989978299 42-43 0.004217361785079078 44-45 0.0026553759387534935 46-47 0.0010413238975503895 48-49 8.330591180403116E-4 50-51 0.004165295590201558 52-53 0.0027074421336310134 54-55 0.005831413826282182 56-57 7.289267282852728E-4 58-59 0.005466950462139546 60-61 0.00197851540534574 62-63 2.082647795100779E-4 64-65 6.768605334077533E-4 66-67 0.016661182360806232 68-69 0.03884138137862953 70-71 0.04743230353342025 72-73 0.01421407120156282 74-75 0.026449626997779897 76-77 0.025252104515596948 78-79 0.053107518775069876 80-81 0.07731829939311644 82-83 0.04909842176950087 84-85 0.018066969622499263 86-87 0.02014961741760004 88-89 0.023169456720496168 90-91 0.03545707871159077 92-93 0.02233639760245586 94-95 0.012391754380849636 96-97 0.019212425909804688 98-99 0.052743055410927234 100-101 0.07185134893097689 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 960316.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.33664468729648 #Duplication Level Percentage of deduplicated Percentage of total 1 85.2893446036579 58.28387637792557 2 9.043498249918498 12.360046532697359 3 2.466301714419147 5.056163518497942 4 1.0189171036857385 2.7851750432152618 5 0.5854830537020108 2.000497370563382 6 0.3512986580897123 1.440394294620043 7 0.2319588527616363 1.1095902792271402 8 0.17042178477103093 0.9316842362298284 9 0.12997035572985854 0.7993564217453585 >10 0.6610970158320943 7.7799882818709465 >50 0.030269437811349487 1.3936119953191064 >100 0.017637898908906192 2.4426326664133904 >500 0.0022807624272786777 1.0858222395432495 >1k 0.0015205082848524518 2.531160742131441 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3829 0.39872292037204415 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3788 0.3944534923920876 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3352 0.34905177045889063 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2699 0.2810533199488502 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2586 0.26928635990653077 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2479 0.2581441942027416 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1678 0.1747341500089554 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1509 0.15713577614035382 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1240 0.12912416329624832 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1200 0.12495886770604676 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.0413238975503896E-4 0.0 0.0 0.0 0.0 6 2.0826477951007793E-4 0.0 0.0 0.0 0.0 7 3.123971692651169E-4 0.0 0.0 0.0 0.0 8 3.123971692651169E-4 0.0 0.0 0.0 0.0 9 3.123971692651169E-4 0.0 0.0 0.0 0.0 10-11 3.123971692651169E-4 0.0 0.0 0.0 1.0413238975503896E-4 12-13 3.123971692651169E-4 0.0 0.0 0.0 1.0413238975503896E-4 14-15 4.1652955902015586E-4 0.0 0.0 0.0 1.0413238975503896E-4 16-17 4.1652955902015586E-4 0.0 0.0 0.0 1.0413238975503896E-4 18-19 4.1652955902015586E-4 0.0 0.0 0.0 1.0413238975503896E-4 20-21 4.1652955902015586E-4 0.0 0.0 1.5619858463255845E-4 1.0413238975503896E-4 22-23 4.1652955902015586E-4 0.0 0.0 3.123971692651169E-4 1.0413238975503896E-4 24-25 4.1652955902015586E-4 0.0 0.0 4.6859575389767533E-4 1.0413238975503896E-4 26-27 4.1652955902015586E-4 0.0 0.0 0.0010413238975503897 1.0413238975503896E-4 28-29 5.206619487751949E-4 0.0 0.0 0.0019264492104682208 1.0413238975503896E-4 30-31 6.247943385302338E-4 0.0 0.0 0.004842156123609312 1.0413238975503896E-4 32-33 6.247943385302338E-4 0.0 0.0 0.007809929231627923 1.0413238975503896E-4 34-35 6.768605334077533E-4 0.0 0.0 0.011402496678176766 1.0413238975503896E-4 36-37 7.289267282852728E-4 0.0 0.0 0.015984321827398482 1.0413238975503896E-4 38-39 9.371915077953507E-4 0.0 0.0 0.024731442566821756 1.0413238975503896E-4 40-41 0.0010413238975503897 0.0 0.0 0.039986837665934964 1.0413238975503896E-4 42-43 0.0010413238975503897 0.0 0.0 0.06711332519712261 1.0413238975503896E-4 44-45 0.0010413238975503897 0.0 0.0 0.09163650298443429 1.0413238975503896E-4 46-47 0.0010413238975503897 0.0 0.0 0.1482845230111755 1.0413238975503896E-4 48-49 0.0010413238975503897 0.0 0.0 0.20003832071942984 1.0413238975503896E-4 50-51 0.0010413238975503897 0.0 0.0 0.23877556970830435 2.0826477951007793E-4 52-53 0.0010413238975503897 0.0 0.0 0.2820425776515231 2.0826477951007793E-4 54-55 0.0010413238975503897 0.0 0.0 0.3268715714410673 2.0826477951007793E-4 56-57 0.0010413238975503897 0.0 0.0 0.42215270806692795 2.0826477951007793E-4 58-59 0.0010413238975503897 0.0 0.0 0.5491942235680756 2.0826477951007793E-4 60-61 0.0010413238975503897 0.0 0.0 0.6697795309044107 2.0826477951007793E-4 62-63 0.0010413238975503897 0.0 0.0 0.7959879872875179 2.0826477951007793E-4 64-65 0.0010413238975503897 0.0 0.0 0.9520303733354437 2.0826477951007793E-4 66-67 0.0010413238975503897 0.0 0.0 1.163731521707438 2.0826477951007793E-4 68-69 0.0010413238975503897 0.0 0.0 1.3982376634357858 2.0826477951007793E-4 70-71 0.0010933900924279092 0.0 0.0 1.5709932980393955 2.0826477951007793E-4 72-73 0.0011454562873054286 0.0 0.0 1.735730738631867 2.0826477951007793E-4 74-75 0.0011454562873054286 0.0 0.0 1.9205136642521836 3.123971692651169E-4 76-77 0.0011454562873054286 0.0 0.0 2.1411181319482333 3.123971692651169E-4 78-79 0.0011454562873054286 0.0 0.0 2.373281294907093 3.123971692651169E-4 80-81 0.0015619858463255846 0.0 0.0 2.689166899229004 3.123971692651169E-4 82-83 0.0015619858463255846 0.0 0.0 3.0573269632079443 3.123971692651169E-4 84-85 0.0015619858463255846 0.0 0.0 3.540240920696937 3.123971692651169E-4 86-87 0.0015619858463255846 0.0 0.0 4.048406982701527 3.123971692651169E-4 88-89 0.0015619858463255846 0.0 0.0 4.646231032285206 3.123971692651169E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 3175 0.0 23.790297 1 TACATGG 3115 0.0 23.638512 2 GAGTACT 3455 0.0 23.37477 12-13 ACATGGG 3195 0.0 22.4507 3 GTGTAAG 410 0.0 22.014874 1 GTACTTT 3705 0.0 21.541082 14-15 GTGTAGC 605 0.0 21.200928 1 AGAGTAC 4980 0.0 20.223871 10-11 GCACCGT 235 1.1305565E-7 20.214172 6 ACAACGC 120 0.004762454 19.792015 8 CATGGGG 1815 0.0 19.630487 4 TATACTG 485 0.0 19.588993 5 AGTACTT 3660 0.0 19.404684 12-13 ATAAGAC 345 5.2750693E-11 19.276705 3 GTATAGC 300 3.7071004E-9 19.002312 1 TAAGGCG 375 9.094947E-12 19.001324 5 CCTATAC 255 2.6717134E-7 18.628748 3 ACACCGT 185 1.5201957E-4 17.974224 6 GTATCAA 8230 0.0 17.894037 1 ATGGGGG 985 0.0 17.843884 5 >>END_MODULE