FastQCFastQC Report
Thu 26 May 2016
SRR936643_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936643_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960316
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT74510.7758904360647954No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28290.29459053061700524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27440.2857392774878269No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26180.272618596378692No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19360.20160030656575542No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC15170.1579688352583941No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14790.15401180444770263No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13830.14401509503121887No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13760.14328616830293361No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC13320.1387043431537119No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13040.1357886362405708No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG12990.13526797429179563No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC11440.11912745387976457No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA11280.11746133564368395No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA11140.11600348218711341No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC10160.10579850799111959No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGT608.2888495E-539.584054
GTACCGT856.4096285E-427.9387746
TAAGGTG5700.025.8338015
GTAAGGT5400.023.7504314
AGGTAAG5950.023.1528852
GAGTACT37500.022.22924412-13
GTAAGCG1105.6494173E-821.58905294-95
GTACATG32600.021.1331861
GGTAAGG6350.020.9463523
AAGGTAA6150.020.8594681
GTACTTT39600.020.75055714-15
TACATGG32350.020.5578562
ACATGGG32550.020.4315413
AGAGTAC55750.019.59574710-11
AGTACTT39450.019.5050412-13
ATAGTAC3305.966285E-1018.7134343
ACTTTTT46650.018.02142716-17
ACGCTCA1851.5220091E-417.9714289
GTGTAGA5950.017.5679171
GTGTAGC4600.017.5592371
Produced by FastQC (version 0.11.5)