##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936643_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 960316 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94474943664377 31.0 31.0 34.0 30.0 34.0 2 32.0620639456179 33.0 31.0 34.0 30.0 34.0 3 32.17944614064537 33.0 31.0 34.0 30.0 34.0 4 35.89137117365534 37.0 35.0 37.0 35.0 37.0 5 35.74460385956289 37.0 35.0 37.0 35.0 37.0 6 35.804301917285564 37.0 35.0 37.0 35.0 37.0 7 35.73148942639715 37.0 35.0 37.0 33.0 37.0 8 35.702884258931434 37.0 35.0 37.0 33.0 37.0 9 37.3155502980269 39.0 37.0 39.0 34.0 39.0 10-11 37.214355482986846 39.0 37.0 39.0 33.0 39.0 12-13 37.10077359952349 39.0 37.0 39.0 33.0 39.0 14-15 38.210558295394435 40.0 38.0 41.0 33.0 41.0 16-17 38.197663060909115 40.0 37.5 41.0 33.0 41.0 18-19 38.32712513381012 40.0 38.0 41.0 33.5 41.0 20-21 38.323421665368485 40.0 38.0 41.0 34.0 41.0 22-23 38.220923112808705 40.0 38.0 41.0 33.0 41.0 24-25 38.09722841231428 40.0 37.0 41.0 33.0 41.0 26-27 37.87538060388455 40.0 37.0 41.0 33.0 41.0 28-29 37.91206436214746 40.0 37.0 41.0 33.0 41.0 30-31 37.804591925991026 40.0 37.0 41.0 33.0 41.0 32-33 37.62371240300068 40.0 37.0 41.0 33.0 41.0 34-35 37.42027468041769 40.0 36.0 41.0 32.0 41.0 36-37 37.235239233752225 39.5 36.0 41.0 31.0 41.0 38-39 37.03349105919302 39.0 36.0 41.0 31.0 41.0 40-41 36.81987699882122 39.0 35.5 41.0 31.0 41.0 42-43 36.560180190687234 39.0 35.0 40.5 30.0 41.0 44-45 36.28616361697608 39.0 35.0 40.0 30.0 41.0 46-47 35.93779755830373 39.0 35.0 40.0 29.5 41.0 48-49 35.53037021147206 38.0 35.0 40.0 28.0 41.0 50-51 35.21733991727723 38.0 34.0 40.0 27.0 41.0 52-53 34.97201181694359 38.0 34.0 40.0 26.5 41.0 54-55 34.65330891081686 38.0 34.0 40.0 26.0 41.0 56-57 34.28910535698665 37.5 33.0 40.0 25.5 41.0 58-59 33.90290487714461 37.0 33.0 40.0 24.0 41.0 60-61 33.49325586577751 36.5 32.5 39.0 23.0 40.5 62-63 33.17929983463777 36.0 32.0 39.0 23.0 40.0 64-65 32.87479485919218 36.0 32.0 39.0 22.0 40.0 66-67 32.41816912349685 35.0 31.0 38.5 20.5 40.0 68-69 31.937958442845897 35.0 31.0 38.0 19.5 40.0 70-71 31.39640857800974 35.0 30.5 37.0 18.0 39.5 72-73 30.825865652556033 34.0 30.0 36.5 14.5 39.0 74-75 30.318522757092456 34.0 29.0 36.0 12.0 39.0 76-77 28.930876399018658 32.5 28.0 34.5 10.0 36.5 78-79 29.735392829027113 34.0 29.0 35.0 8.0 37.0 80-81 29.566522894547212 34.0 29.0 35.0 7.0 37.0 82-83 29.16110738548561 34.0 29.0 35.0 4.5 36.5 84-85 28.722584024425295 33.0 29.0 35.0 2.0 36.0 86-87 28.33740872796038 33.0 27.5 35.0 2.0 35.5 88-89 27.841780205682298 33.0 27.0 35.0 2.0 35.0 90-91 27.275907097247156 32.5 26.0 34.5 2.0 35.0 92-93 26.703243515676093 32.0 25.0 34.0 2.0 35.0 94-95 25.955553692742804 32.0 24.5 34.0 2.0 35.0 96-97 25.145930610340763 31.5 21.5 34.0 2.0 35.0 98-99 23.985995755563792 31.0 9.5 34.0 2.0 35.0 100-101 20.27250457141191 27.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2779511613601251 1101 2 0.4448884548855183 1101 3 0.3315398066393911 1101 4 0.20967014136311235 1101 5 0.24101637396076825 1101 6 0.30464389893401034 1101 7 0.29319423437832626 1101 8 0.2342595728128245 1101 9 0.33206038564789964 1101 10-11 0.34207545026223585 1101 12-13 0.33851163915433347 1101 14-15 0.4533682793350664 1101 16-17 0.4274859988497255 1101 18-19 0.44139920376488107 1101 20-21 0.4168999447329824 1101 22-23 0.4566362744514336 1101 24-25 0.46594753595817195 1101 26-27 0.2851816267900986 1101 28-29 0.4057142824343529 1101 30-31 0.5191015287103014 1101 32-33 0.42813737284878073 1101 34-35 0.5700393636072221 1101 36-37 0.6591689943874215 1101 38-39 0.5440568133616281 1101 40-41 0.4532595723364139 1101 42-43 0.6255434867325391 1101 44-45 0.8673571147258201 1101 46-47 0.6904645142604764 1101 48-49 0.8135920490917314 1101 50-51 0.7187870022735581 1101 52-53 0.5902689447278817 1101 54-55 0.6798711687929995 1101 56-57 0.7665786152745611 1101 58-59 0.7261851328496434 1101 60-61 0.7457436752896456 1101 62-63 0.604562828882564 1101 64-65 0.6879386051775072 1101 66-67 0.7709941883660463 1101 68-69 0.5908068815778513 1101 70-71 0.48800780858182335 1101 72-73 0.7376468703947694 1101 74-75 0.7367208078077425 1101 76-77 0.8082799730951784 1101 78-79 0.751461484914369 1101 80-81 0.800162332886508 1101 82-83 0.7070089078739876 1101 84-85 0.6099465235570776 1101 86-87 0.6934863256444395 1101 88-89 0.39505397340209925 1101 90-91 0.602674047879237 1101 92-93 0.6301832468906134 1101 94-95 0.4851965671899272 1101 96-97 0.5524752509851645 1101 98-99 0.3060740666268451 1101 100-101 0.44357692137632654 1102 1 0.16365578871981157 1102 2 0.3454257953441946 1102 3 0.24030245383481486 1102 4 0.17158657727908633 1102 5 0.14466252880183106 1102 6 0.12006980297699954 1102 7 0.18855041349964807 1102 8 0.15281251613339464 1102 9 0.25176972796434427 1102 10-11 0.23513000148888352 1102 12-13 0.2694513382671957 1102 14-15 0.3415573714413327 1102 16-17 0.3604136812897494 1102 18-19 0.219287996630122 1102 20-21 0.2030210991331174 1102 22-23 0.0919328993184152 1102 24-25 0.1622275996865099 1102 26-27 0.1482259485594497 1102 28-29 0.22854654872946867 1102 30-31 0.3338678091267724 1102 32-33 0.27889458784963495 1102 34-35 0.3726743307142186 1102 36-37 0.2616646292310705 1102 38-39 0.4561516768806726 1102 40-41 0.4575809301499021 1102 42-43 0.4516238996733861 1102 44-45 0.5132756200492494 1102 46-47 0.4558016454268099 1102 48-49 0.5466767007904778 1102 50-51 0.449681139148403 1102 52-53 0.5094547622320107 1102 54-55 0.5054994867527469 1102 56-57 0.5846588871194456 1102 58-59 0.36270076804603235 1102 60-61 0.33197711856025336 1102 62-63 0.48145277991049795 1102 64-65 0.2703357080070745 1102 66-67 0.3680247062470272 1102 68-69 0.20875899125644182 1102 70-71 -0.0427693362237882 1102 72-73 -0.029780244721901994 1102 74-75 0.1895735918074628 1102 76-77 0.21022386393393333 1102 78-79 0.20857654279310722 1102 80-81 0.3276930947459178 1102 82-83 0.36921988161249786 1102 84-85 0.33554956182347695 1102 86-87 0.3580874062043513 1102 88-89 0.01828013259362038 1102 90-91 -0.057429892813427585 1102 92-93 0.328411022806705 1102 94-95 0.32659030135337375 1102 96-97 0.20371131041008184 1102 98-99 0.08452136434520341 1102 100-101 0.2719231003212812 1103 1 0.16063702442388106 1103 2 0.2133091439153567 1103 3 0.1749469101122969 1103 4 0.06962139990073268 1103 5 0.17175069721498204 1103 6 0.15576315760979753 1103 7 0.1556306783364363 1103 8 0.1616263433732712 1103 9 0.1469883991588219 1103 10-11 0.17903704739325832 1103 12-13 0.07256487850008853 1103 14-15 0.20266340327667365 1103 16-17 0.2144297697562152 1103 18-19 0.19824857586850442 1103 20-21 0.24763963435928815 1103 22-23 0.1431793870829452 1103 24-25 0.1282068896372479 1103 26-27 0.06214394560564784 1103 28-29 0.17640688985586195 1103 30-31 0.2865766259680882 1103 32-33 0.13982178452477 1103 34-35 0.21859025809509092 1103 36-37 0.3166232861589222 1103 38-39 0.14705754059756515 1103 40-41 0.17043880978111048 1103 42-43 0.31090892741962506 1103 44-45 0.32067584577003316 1103 46-47 0.30414139204351187 1103 48-49 0.21100243530398188 1103 50-51 0.19957045558951592 1103 52-53 0.20484808237039687 1103 54-55 0.2667861956834514 1103 56-57 0.2282611764774174 1103 58-59 0.21935534492003939 1103 60-61 0.017790366427789195 1103 62-63 0.31115766840644454 1103 64-65 0.34913285736121935 1103 66-67 0.2011405237815751 1103 68-69 0.2593214512780797 1103 70-71 -0.03402114885544094 1103 72-73 0.35695782204992454 1103 74-75 0.25591992920310247 1103 76-77 0.08645620228737982 1103 78-79 -0.017873646256422404 1103 80-81 0.13486743223532827 1103 82-83 0.1843575746389341 1103 84-85 0.29069241226838827 1103 86-87 0.17843827810948554 1103 88-89 0.06428899397627674 1103 90-91 0.08347446386559554 1103 92-93 0.20534915722778635 1103 94-95 0.12944680790564078 1103 96-97 0.49703831418437616 1103 98-99 0.349247301136689 1103 100-101 -0.19898152427558458 1104 1 0.1848405578837209 1104 2 0.2579554647187159 1104 3 0.17148956311840635 1104 4 0.1940416971266501 1104 5 0.0944937058820301 1104 6 0.17486537680777303 1104 7 0.19203050576722092 1104 8 0.08266575719915181 1104 9 0.12264526702762168 1104 10-11 0.2027260299714797 1104 12-13 0.21679363091587334 1104 14-15 0.2308128790801547 1104 16-17 0.13518384810684836 1104 18-19 0.15753223826420282 1104 20-21 0.19678468018483386 1104 22-23 0.15922972678473712 1104 24-25 0.17499629557124052 1104 26-27 0.16475387955934906 1104 28-29 0.15778467262209261 1104 30-31 0.3336438262430761 1104 32-33 0.2845175611388413 1104 34-35 0.43423285042136683 1104 36-37 0.4815172016912044 1104 38-39 0.3531763818584537 1104 40-41 0.4085169780220639 1104 42-43 0.36164462644770623 1104 44-45 0.5292536229242586 1104 46-47 0.5178315320981639 1104 48-49 0.617289812146744 1104 50-51 0.7067750448081469 1104 52-53 0.7488272758548504 1104 54-55 0.5416743939837119 1104 56-57 0.8835222575603936 1104 58-59 0.8391825652579499 1104 60-61 0.7470006869084571 1104 62-63 0.6684918599091674 1104 64-65 0.8430131392242686 1104 66-67 0.7821924593978338 1104 68-69 0.7085266508024581 1104 70-71 0.4181606941977698 1104 72-73 0.5041982930376037 1104 74-75 0.6320317203803256 1104 76-77 0.5434335052270214 1104 78-79 0.7739845593745258 1104 80-81 0.6179030369115317 1104 82-83 0.55943149529217 1104 84-85 0.6536152857562136 1104 86-87 0.6422397814258289 1104 88-89 0.6057889080424204 1104 90-91 0.7674004485097434 1104 92-93 0.9037127310940711 1104 94-95 0.8096979802889557 1104 96-97 0.9103371921337491 1104 98-99 0.6784253525777437 1104 100-101 0.20640810684018618 1105 1 0.078696888259838 1105 2 0.222137361985002 1105 3 0.151256323617055 1105 4 0.04183035357848297 1105 5 0.1304324497371283 1105 6 0.11201053187675569 1105 7 0.07163887350105824 1105 8 0.0898812452212141 1105 9 0.13040815387938665 1105 10-11 0.21049260991879493 1105 12-13 0.06819249344245293 1105 14-15 0.1795957989235646 1105 16-17 0.24015188231312123 1105 18-19 0.18509545174973852 1105 20-21 0.12038438145444275 1105 22-23 0.15840288372488232 1105 24-25 0.12528189496065067 1105 26-27 0.2992814505693602 1105 28-29 0.09747231880712093 1105 30-31 0.16066948915372592 1105 32-33 0.1466904144244623 1105 34-35 -0.09990612486771511 1105 36-37 -0.0012975936702659396 1105 38-39 -0.27284681078162265 1105 40-41 0.05549069138860574 1105 42-43 -0.02037081889859138 1105 44-45 -0.014074184443884974 1105 46-47 -0.08580370495754153 1105 48-49 -0.012249617803441026 1105 50-51 0.0680262655814019 1105 52-53 0.1537419653475851 1105 54-55 0.10914437732375148 1105 56-57 0.39646491609677526 1105 58-59 0.41758452177790417 1105 60-61 0.1534738627244252 1105 62-63 0.15807815113846857 1105 64-65 0.03079473776016073 1105 66-67 0.24643549791271369 1105 68-69 0.17161621247305092 1105 70-71 0.012698195531385181 1105 72-73 0.07530802721679208 1105 74-75 0.10506760906221757 1105 76-77 -0.024720931822116654 1105 78-79 0.16985403717081127 1105 80-81 0.09698206094947892 1105 82-83 0.07932825275427646 1105 84-85 0.14905677481234392 1105 86-87 0.1448015587075453 1105 88-89 0.10600433861632652 1105 90-91 0.3277985642103225 1105 92-93 0.1308143076355286 1105 94-95 0.14909592364507418 1105 96-97 0.1173803356966836 1105 98-99 0.14416819823097526 1105 100-101 0.3240011933653122 1106 1 0.022142351981717212 1106 2 0.1940671943391017 1106 3 0.12295795676823218 1106 4 0.14130743871400142 1106 5 0.1536482484509918 1106 6 0.12351210484109032 1106 7 0.1369810223935417 1106 8 0.06693861927579547 1106 9 0.03363725588498312 1106 10-11 0.0036811135649870153 1106 12-13 0.11659820830560363 1106 14-15 0.1649519062782474 1106 16-17 0.1757097599605899 1106 18-19 0.08083395656191072 1106 20-21 0.04784090978390054 1106 22-23 0.06650939667449762 1106 24-25 0.2832765292256241 1106 26-27 0.13902536775581353 1106 28-29 0.1262434633232914 1106 30-31 0.00566309846558255 1106 32-33 0.027943544529101416 1106 34-35 0.09236669477495951 1106 36-37 0.03445777900512326 1106 38-39 0.23812007645910427 1106 40-41 0.11486856816991775 1106 42-43 0.2796628279911815 1106 44-45 0.19218846784160348 1106 46-47 0.19052637436250563 1106 48-49 0.13946731746460017 1106 50-51 0.1417959609493451 1106 52-53 0.20891037191750428 1106 54-55 0.222419463412038 1106 56-57 0.25298563891637116 1106 58-59 0.054688934850666726 1106 60-61 -0.15142858905669243 1106 62-63 3.983318322582363E-4 1106 64-65 -0.01157230427846656 1106 66-67 -0.12212176120942786 1106 68-69 0.08126626261024938 1106 70-71 0.12903092884702616 1106 72-73 0.02580184807098007 1106 74-75 -0.28997547117173283 1106 76-77 -0.3723746442511455 1106 78-79 -0.4803185280658404 1106 80-81 -0.48819509603269395 1106 82-83 -0.43713096177407706 1106 84-85 -0.1825580315958888 1106 86-87 -0.1253110639842241 1106 88-89 -0.13802389367824475 1106 90-91 0.12318827403643695 1106 92-93 -0.18194324931155492 1106 94-95 0.05044418672155615 1106 96-97 -0.11814594940907241 1106 98-99 0.2700700292658418 1106 100-101 -0.2809171379710449 1107 1 0.14882140119847165 1107 2 0.20925026163299876 1107 3 0.18961289622868804 1107 4 0.10398711395662019 1107 5 -0.0028918463065537026 1107 6 0.17408438819281002 1107 7 0.17244461135841505 1107 8 0.04990916372427989 1107 9 0.10951184893583843 1107 10-11 0.15159039413968145 1107 12-13 0.24011479217043075 1107 14-15 0.18594188081036833 1107 16-17 0.24918954540445526 1107 18-19 0.24037677660146528 1107 20-21 0.0620574526466271 1107 22-23 0.1257700523719123 1107 24-25 0.06701434338648227 1107 26-27 0.14454998130772623 1107 28-29 0.1075390005608341 1107 30-31 0.12298959294064815 1107 32-33 0.021054349813070417 1107 34-35 0.027218291722959975 1107 36-37 0.21359365636613603 1107 38-39 0.06371399262595645 1107 40-41 0.047499972162256654 1107 42-43 -0.05843834761812161 1107 44-45 -0.18454846289846216 1107 46-47 0.04762142035082917 1107 48-49 -0.22314522814539117 1107 50-51 -0.1369708327764272 1107 52-53 -0.2848804604252706 1107 54-55 -0.07931336065011863 1107 56-57 -0.027436437031951755 1107 58-59 -0.13338915282141528 1107 60-61 -0.2610419663498007 1107 62-63 -0.48576151688677527 1107 64-65 -0.2379152526274524 1107 66-67 -0.08817537954836041 1107 68-69 -0.22664986027190182 1107 70-71 -0.31380152774829995 1107 72-73 -0.0974153040028618 1107 74-75 -0.08519161177403589 1107 76-77 -0.362371811613464 1107 78-79 -0.018391959347216158 1107 80-81 0.08108520704660194 1107 82-83 0.11856820175707483 1107 84-85 0.1300823876862225 1107 86-87 0.3007541823970463 1107 88-89 0.1454549087498016 1107 90-91 0.2042670304179559 1107 92-93 0.24055015736855623 1107 94-95 0.21294381485702019 1107 96-97 0.364345374829643 1107 98-99 0.7932168181024046 1107 100-101 0.610198339224926 1108 1 0.01492195637589333 1108 2 0.06220861102574915 1108 3 -0.04409629577084928 1108 4 -0.04433874320615416 1108 5 -0.05560991950468974 1108 6 -0.027144054604235635 1108 7 -0.014598515348325236 1108 8 0.0192440168933814 1108 9 0.14265848301897677 1108 10-11 -0.08141478040907657 1108 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#Quality Count 3 7.0 4 17.0 5 28.0 6 66.0 7 173.0 8 428.0 9 732.0 10 1220.0 11 1915.0 12 2676.0 13 3726.0 14 4832.0 15 6233.0 16 7683.0 17 9564.0 18 8770.0 19 6859.0 20 6589.0 21 6893.0 22 7244.0 23 8080.0 24 9333.0 25 11197.0 26 13590.0 27 16321.0 28 19973.0 29 24778.0 30 30506.0 31 37034.0 32 46150.0 33 59279.0 34 78247.0 35 106880.0 36 148314.0 37 179632.0 38 91827.0 39 3520.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.56174769374327 19.29465723265855 14.652046314675687 22.491548758922487 2 15.99466788860886 21.0663181354273 38.57449334527504 24.3645206306888 3 20.838375360057984 23.708008855916407 29.49080563429564 25.96281014972997 4 13.49305853489893 16.759587469124746 36.69437976666014 33.052974229316185 5 13.81756872856707 37.65562142871276 33.9966238415934 14.530186001126774 6 32.537623032418495 36.39406195460661 16.314838032480974 14.753476980493922 7 29.0562992351468 32.89254046849211 19.89378485184549 18.1573754445156 8 26.645104050384045 34.23738732734634 19.012312767623584 20.10519585464603 9 25.92407082668622 17.59618708841673 19.844197118448513 36.63554496644855 10-11 25.458338713506805 26.298322635465826 26.620716514147425 21.622622136879944 12-13 26.81419449431229 23.697407936554217 26.987002195110776 22.501395374022717 14-15 22.910914379940152 25.763389084630795 25.84320735098638 25.482489184442674 16-17 22.1381955414948 28.580763675481137 26.64840457492994 22.632636208094123 18-19 22.09127839107439 27.704148629637 28.776061056365087 21.428511922923523 20-21 23.023398694171675 25.983067967635453 28.890618654392853 22.102914683800023 22-23 22.27845385907831 26.184469967359618 29.01286923489048 22.52420693867159 24-25 23.059268604191647 26.19318983520503 28.882621266262248 21.864920294341072 26-27 22.52232598436348 26.088391737719668 29.383973608687143 22.005308669229713 28-29 21.871602680784243 27.4063953948492 28.88002490846763 21.841977015898934 30-31 22.404396906628733 26.4420971736775 28.641852149016227 22.511653770677537 32-33 22.355418396414795 26.587207162081455 28.751472878469237 22.305901563034514 34-35 23.00939280024159 26.45234351407358 28.77164665576741 21.766617029917423 36-37 23.197012628226958 25.885105731592645 29.018992845011343 21.898888795169054 38-39 22.60502543333519 26.74251800692628 28.89569494785502 21.756761611883505 40-41 22.468969833351643 26.82670434872706 28.639389867184274 22.064935950737024 42-43 22.37422309555905 27.086024545416205 27.79783224331108 22.74192011571366 44-45 22.758677426896444 26.490513811559214 28.131676220458008 22.619132541086337 46-47 23.50148284523011 27.263213358936017 27.478611207144315 21.756692588689557 48-49 23.329098501494343 27.176373803746785 27.24577992064897 22.248747774109905 50-51 22.585173356215275 27.530234309061363 27.967549436811634 21.917042897911728 52-53 23.173266465828117 27.823341910451553 26.966272244188577 22.037119379531752 54-55 22.462311767100935 27.638644900152347 27.159374079076304 22.73966925367042 56-57 23.021021174267613 27.489107720955612 27.0819523254827 22.40791877929407 58-59 23.522027380170748 27.089582507847858 26.810813568976567 22.57757654300482 60-61 22.753564706219265 27.477643297675424 27.82139630785732 21.94739568824799 62-63 22.337797845288105 27.530214075812705 27.873799611377116 22.258188467522082 64-65 22.172890699929084 28.273227788058314 27.234263232817824 22.319618279194778 66-67 22.485302915362183 27.406841231915337 27.057995365035957 23.049860487686523 68-69 22.440126962704706 27.403668922254088 27.216119848127946 22.94008426691326 70-71 23.065988589602135 27.484979398905196 26.868847072863776 22.58018493862889 72-73 22.794765054892988 27.10081200486318 27.53625905011832 22.56816389012551 74-75 22.310665487114573 28.322371795305706 26.807716520099163 22.559246197480558 76-77 22.61842405334172 27.434372002886604 26.942754287987388 23.004449655784292 78-79 22.730079749732884 27.83362526998065 26.672474157879062 22.763820822407403 80-81 23.11111967338846 27.59177098037092 26.587693542903175 22.70941580333745 82-83 22.807881696450654 27.63951595141186 26.945131891837292 22.607470460300195 84-85 22.406184003898847 28.053679953013898 26.878194367499614 22.661941675587638 86-87 22.307316626158745 28.14368545026273 26.51035190866245 23.03864601491608 88-89 21.91628757486597 28.315957677786983 26.02307925808563 23.744675489261425 90-91 22.28607313092896 27.750637091592267 26.365646313408448 23.597643464070327 92-93 22.53293789805601 27.857203850495477 26.646790767808127 22.963067483640394 94-95 22.167303270630967 27.933498142254955 26.662077842016696 23.237120745097386 96-97 21.77821189549657 27.84232849081497 27.20107923145891 23.178380382229545 98-99 21.79920868931814 28.32697511364873 26.017553788872394 23.856262408160735 100-101 21.2376234530618 28.28116473876478 26.167415056379156 24.31379675179426 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 150.0 1 113.0 2 96.5 3 112.5 4 114.5 5 121.0 6 170.5 7 328.5 8 511.5 9 637.0 10 650.0 11 661.5 12 727.0 13 784.0 14 912.0 15 1038.0 16 1148.0 17 1275.5 18 1379.5 19 1471.5 20 1630.0 21 1905.5 22 2236.0 23 2658.0 24 3204.5 25 4037.0 26 5238.5 27 6592.0 28 7954.0 29 9542.5 30 11421.0 31 13437.0 32 15667.0 33 16944.0 34 18190.5 35 19918.0 36 21902.0 37 23849.5 38 24899.5 39 25668.5 40 26608.0 41 27942.5 42 28593.0 43 29701.0 44 31032.5 45 32274.5 46 33389.0 47 37224.5 48 46065.0 49 45336.5 50 36924.5 51 33313.5 52 32166.5 53 32067.0 54 31816.5 55 30294.0 56 27131.5 57 24526.5 58 23555.0 59 20995.0 60 16922.0 61 14061.5 62 11964.5 63 9147.5 64 6348.0 65 4624.5 66 3558.0 67 2920.5 68 2400.5 69 2009.0 70 1547.5 71 1134.0 72 877.5 73 669.0 74 546.5 75 429.5 76 324.0 77 209.5 78 133.0 79 91.5 80 65.5 81 55.0 82 38.5 83 24.0 84 16.5 85 10.0 86 5.5 87 1.5 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010621503755013974 2 0.009996709416483741 3 0.0056231490467721044 4 0.0 5 0.005519016657017066 6 0.0 7 1.0413238975503895E-4 8 0.0012495886770604676 9 0.0 10-11 0.0 12-13 0.0 14-15 9.8925770267287E-4 16-17 0.004061163200446519 18-19 0.002395044964365896 20-21 6.247943385302338E-4 22-23 2.603309743875974E-4 24-25 1.5619858463255845E-4 26-27 0.0 28-29 0.0 30-31 4.6859575389767533E-4 32-33 0.0038008322260589225 34-35 6.247943385302338E-4 36-37 7.289267282852728E-4 38-39 0.006300009580179857 40-41 5.2066194877519476E-5 42-43 0.001509919651448065 44-45 0.005362818072384507 46-47 0.0 48-49 0.0027074421336310134 50-51 0.0025512435489984547 52-53 9.371915077953507E-4 54-55 3.123971692651169E-4 56-57 0.012235555796217079 58-59 0.002342978769488377 60-61 0.0034884350567938056 62-63 2.082647795100779E-4 64-65 0.003436368861916286 66-67 6.768605334077533E-4 68-69 0.003540501251671325 70-71 0.005362818072384507 72-73 0.004009097005569 74-75 0.006716539139200013 76-77 0.0017702506258356625 78-79 0.006456208164812416 80-81 0.0032801702772837275 82-83 0.004217361785079078 84-85 0.0033322364721612464 86-87 0.0010413238975503895 88-89 0.001718184430958143 90-91 0.009684312247218623 92-93 0.0024991773541209353 94-95 0.0012495886770604676 96-97 3.123971692651169E-4 98-99 0.0010933900924279092 100-101 0.0033322364721612464 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 960316.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.44316232677788 #Duplication Level Percentage of deduplicated Percentage of total 1 83.95335932218167 54.941733219998454 2 9.671437227859201 12.658588728720648 3 2.7261517030017046 5.352239652808875 4 1.1639149725779894 3.0468110593995434 5 0.6501054251104958 2.1272477432512558 6 0.40488731662806426 1.5898263831686354 7 0.26162664557749366 1.198517252487681 8 0.20405458698058168 1.0683181967435058 9 0.15492584755337344 0.9124953651044154 >10 0.7470371054087436 8.665020014876152 >50 0.03759959899779568 1.6977400502284044 >100 0.02030083964928088 2.546458765914171 >500 0.002061803798515058 0.9078777792304846 >1k 0.0023790043829019897 2.5137637973228655 >5k 1.5860029219346602E-4 0.7733619907449801 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7451 0.7758904360647954 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2829 0.29459053061700524 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2744 0.2857392774878269 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2618 0.272618596378692 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1936 0.20160030656575542 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1517 0.1579688352583941 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1479 0.15401180444770263 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1383 0.14401509503121887 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1376 0.14328616830293361 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1332 0.1387043431537119 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1304 0.1357886362405708 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1299 0.13526797429179563 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1144 0.11912745387976457 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1128 0.11746133564368395 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1114 0.11600348218711341 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1016 0.10579850799111959 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0413238975503896E-4 0.0 10-11 0.0 0.0 0.0 1.0413238975503896E-4 0.0 12-13 0.0 0.0 0.0 1.0413238975503896E-4 0.0 14-15 0.0 0.0 0.0 1.0413238975503896E-4 5.206619487751948E-5 16-17 0.0 0.0 0.0 1.0413238975503896E-4 1.0413238975503896E-4 18-19 5.206619487751948E-5 0.0 0.0 1.5619858463255845E-4 1.0413238975503896E-4 20-21 1.0413238975503896E-4 0.0 0.0 3.644633641426364E-4 1.0413238975503896E-4 22-23 1.0413238975503896E-4 0.0 0.0 5.206619487751949E-4 1.0413238975503896E-4 24-25 1.0413238975503896E-4 0.0 0.0 7.289267282852728E-4 1.0413238975503896E-4 26-27 1.0413238975503896E-4 0.0 0.0 0.0014578534565705455 1.0413238975503896E-4 28-29 2.0826477951007793E-4 0.0 0.0 0.002395044964365896 1.0413238975503896E-4 30-31 2.0826477951007793E-4 0.0 0.0 0.005206619487751948 1.0413238975503896E-4 32-33 2.6033097438759743E-4 0.0 0.0 0.00812232640089304 1.0413238975503896E-4 34-35 3.123971692651169E-4 0.0 0.0 0.011662827652564364 1.0413238975503896E-4 36-37 3.123971692651169E-4 0.0 0.0 0.016400851386418638 1.0413238975503896E-4 38-39 4.1652955902015586E-4 0.0 0.0 0.02535623690535199 1.5619858463255845E-4 40-41 4.1652955902015586E-4 0.0 0.0 0.04055956580958768 2.0826477951007793E-4 42-43 4.1652955902015586E-4 0.0 0.0 0.0675819209510203 2.0826477951007793E-4 44-45 4.1652955902015586E-4 0.0 0.0 0.092209231128087 2.0826477951007793E-4 46-47 5.206619487751949E-4 0.0 0.0 0.14870105257019567 3.123971692651169E-4 48-49 6.247943385302338E-4 0.0 0.0 0.20003832071942984 3.123971692651169E-4 50-51 6.247943385302338E-4 0.0 0.0 0.23846317253903923 3.123971692651169E-4 52-53 6.247943385302338E-4 5.206619487751948E-5 0.0 0.2817822466771355 3.123971692651169E-4 54-55 6.247943385302338E-4 1.0413238975503896E-4 0.0 0.3270798362205774 3.123971692651169E-4 56-57 6.247943385302338E-4 1.0413238975503896E-4 0.0 0.42345436293886596 3.123971692651169E-4 58-59 6.247943385302338E-4 1.0413238975503896E-4 0.0 0.5500272826861159 3.123971692651169E-4 60-61 6.247943385302338E-4 1.0413238975503896E-4 0.0 0.6704563914378184 3.123971692651169E-4 62-63 6.247943385302338E-4 1.0413238975503896E-4 0.0 0.7965607154311706 3.123971692651169E-4 64-65 6.247943385302338E-4 1.0413238975503896E-4 0.0 0.9522386381149538 3.123971692651169E-4 66-67 8.330591180403117E-4 1.0413238975503896E-4 0.0 1.1628984625893977 3.123971692651169E-4 68-69 8.330591180403117E-4 1.0413238975503896E-4 0.0 1.3970922071484804 3.123971692651169E-4 70-71 8.330591180403117E-4 1.5619858463255845E-4 0.0 1.5705247022854976 3.123971692651169E-4 72-73 8.330591180403117E-4 2.0826477951007793E-4 0.0 1.7364075991652748 3.123971692651169E-4 74-75 8.330591180403117E-4 2.0826477951007793E-4 0.0 1.9216070543446115 3.123971692651169E-4 76-77 8.330591180403117E-4 2.0826477951007793E-4 0.0 2.142367720625294 3.123971692651169E-4 78-79 8.330591180403117E-4 2.0826477951007793E-4 0.0 2.376457332794622 3.123971692651169E-4 80-81 9.371915077953507E-4 2.0826477951007793E-4 0.0 2.693332194819205 3.123971692651169E-4 82-83 9.371915077953507E-4 2.0826477951007793E-4 0.0 3.0624815165008186 3.123971692651169E-4 84-85 9.892577026728703E-4 2.0826477951007793E-4 0.0 3.5435731571690985 3.123971692651169E-4 86-87 0.0010413238975503897 2.0826477951007793E-4 0.0 4.047834254557874 3.123971692651169E-4 88-89 0.0010413238975503897 2.0826477951007793E-4 0.0 4.637588043935538 3.123971692651169E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAACGT 60 8.2888495E-5 39.58405 4 GTACCGT 85 6.4096285E-4 27.938774 6 TAAGGTG 570 0.0 25.833801 5 GTAAGGT 540 0.0 23.750431 4 AGGTAAG 595 0.0 23.152885 2 GAGTACT 3750 0.0 22.229244 12-13 GTAAGCG 110 5.6494173E-8 21.589052 94-95 GTACATG 3260 0.0 21.133186 1 GGTAAGG 635 0.0 20.946352 3 AAGGTAA 615 0.0 20.859468 1 GTACTTT 3960 0.0 20.750557 14-15 TACATGG 3235 0.0 20.557856 2 ACATGGG 3255 0.0 20.431541 3 AGAGTAC 5575 0.0 19.595747 10-11 AGTACTT 3945 0.0 19.50504 12-13 ATAGTAC 330 5.966285E-10 18.713434 3 ACTTTTT 4665 0.0 18.021427 16-17 ACGCTCA 185 1.5220091E-4 17.971428 9 GTGTAGA 595 0.0 17.567917 1 GTGTAGC 460 0.0 17.559237 1 >>END_MODULE