FastQCFastQC Report
Thu 26 May 2016
SRR936642_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936642_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154086
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45000.38991894884783285No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43230.3745821368598181No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38990.3378431070128223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30850.2673111015990143No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27690.23993012652436646No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26920.23325818006630356No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT18440.15978012037231193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17830.1544945523990413No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15120.13101276681287183No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13740.11905525238153829No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC11770.10198546728753316No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG1000.001650942723.7566851
GAGTACT38800.023.51126112-13
GTACATG39400.022.912541
GTGTAGC7300.022.7803841
TACATGG38650.022.6195622
GTACTTT41150.022.1094114-15
ACATGGG39300.021.6409513
ACGTTCG1100.002871231221.598867
CGTTCGA1100.002871231221.598868
TACCTGG8650.020.8729252
CATGGGG22600.020.3929084
AGTACTT42250.020.01703512-13
AGAGTAC60800.019.8892710-11
GTATAGC4350.019.6607071
CCTACAC4650.019.4140643
GTATCAA99700.019.0148811
TAGCGCG1250.006021358519.005354
GTGTAGG4250.019.0053481
GTACTAG2803.4769982E-818.6659681
TCGTTAC1051.9579238E-518.09444822-23
Produced by FastQC (version 0.11.5)