##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936642_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1154086 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.338795375734563 31.0 31.0 34.0 30.0 34.0 2 31.394744412461463 31.0 31.0 34.0 28.0 34.0 3 31.42735550036999 31.0 31.0 34.0 28.0 34.0 4 35.05702261356606 37.0 35.0 37.0 32.0 37.0 5 34.99008912680684 37.0 35.0 37.0 32.0 37.0 6 35.0914455248569 37.0 35.0 37.0 32.0 37.0 7 34.90101604213204 37.0 35.0 37.0 32.0 37.0 8 34.90016861828321 37.0 35.0 37.0 32.0 37.0 9 36.40914281951258 39.0 37.0 39.0 32.0 39.0 10-11 36.284047722613394 39.0 36.0 39.0 32.0 39.0 12-13 36.19649358886599 39.0 35.0 39.0 31.5 39.0 14-15 37.280798398039664 40.0 37.0 41.0 32.0 41.0 16-17 37.22983209223576 40.0 37.0 41.0 31.5 41.0 18-19 37.196137896135994 39.5 37.0 41.0 31.0 41.0 20-21 37.08463624028019 39.0 36.0 41.0 31.0 41.0 22-23 36.9911774339174 39.0 36.0 41.0 31.0 41.0 24-25 36.83450193486448 39.0 36.0 41.0 30.5 41.0 26-27 36.62655685971409 39.0 36.0 40.5 30.0 41.0 28-29 36.708219751387674 39.0 36.0 41.0 30.0 41.0 30-31 36.65916881410917 39.0 36.0 41.0 30.0 41.0 32-33 36.496285805390585 39.0 35.0 41.0 30.0 41.0 34-35 36.30701481518709 39.0 35.0 40.5 30.0 41.0 36-37 36.045663841342844 38.5 35.0 40.0 29.5 41.0 38-39 35.85717355552359 38.0 35.0 40.0 28.5 41.0 40-41 35.713957192098334 38.0 35.0 40.0 27.5 41.0 42-43 35.44390019461288 38.0 35.0 40.0 27.0 41.0 44-45 35.16630519735964 38.0 34.0 40.0 26.0 41.0 46-47 34.82543242011427 38.0 34.0 40.0 24.5 41.0 48-49 34.577766301644765 38.0 33.5 40.0 24.0 41.0 50-51 33.87657895512119 37.5 33.0 39.5 23.5 40.5 52-53 34.0679126165641 38.0 33.0 39.5 23.5 40.5 54-55 34.425894170798365 38.0 34.0 40.0 23.5 41.0 56-57 34.33664432286675 38.0 34.0 40.0 23.0 41.0 58-59 34.08871955816117 38.0 33.5 40.0 23.0 41.0 60-61 33.74614023564968 37.0 33.0 40.0 22.0 41.0 62-63 33.43913668478779 37.0 33.0 40.0 21.5 41.0 64-65 33.129863805643595 36.0 32.0 39.5 20.5 41.0 66-67 32.74127621338444 36.0 32.0 39.0 19.5 41.0 68-69 32.34465195834626 35.0 31.5 39.0 18.0 40.5 70-71 31.93024350005112 35.0 31.0 38.5 17.0 40.0 72-73 31.5238959661585 35.0 31.0 37.5 14.0 40.0 74-75 31.111867313181165 35.0 31.0 37.0 11.0 39.0 76-77 30.698564058484376 35.0 30.0 36.5 7.5 39.0 78-79 30.2464404732403 34.0 30.0 36.0 7.0 38.5 80-81 29.819848780766772 34.0 29.0 35.5 3.5 37.0 82-83 29.40728637207279 34.0 29.0 35.0 2.0 37.0 84-85 29.032116324086765 34.0 29.0 35.0 2.0 36.5 86-87 28.72325112686576 34.0 29.0 35.0 2.0 36.0 88-89 28.332360413348745 34.0 28.5 35.0 2.0 36.0 90-91 27.96797422375802 34.0 27.0 35.0 2.0 35.0 92-93 27.59995702226697 34.0 27.0 35.0 2.0 35.0 94-95 27.171423533428182 33.0 26.0 35.0 2.0 35.0 96-97 26.59229511492211 33.0 25.0 35.0 2.0 35.0 98-99 25.937878113069562 33.0 24.5 35.0 2.0 35.0 100-101 23.95028793348156 31.0 10.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34194508660045386 1101 2 0.3362270865421735 1101 3 0.400281523750337 1101 4 0.31795334667085484 1101 5 0.37175306390025753 1101 6 0.2493891805406392 1101 7 0.22274670544410924 1101 8 0.3580962290482148 1101 9 0.5241126722926097 1101 10-11 0.4791644702995441 1101 12-13 0.513383079306287 1101 14-15 0.5850688459374922 1101 16-17 0.6820352486234498 1101 18-19 0.5811986430039937 1101 20-21 0.6207259926920159 1101 22-23 0.49024872984261947 1101 24-25 0.5940808871141314 1101 26-27 0.6085957011345542 1101 28-29 0.6259642232883351 1101 30-31 0.439006008263398 1101 32-33 0.4987450873408932 1101 34-35 0.4530501136752676 1101 36-37 0.7125076027245072 1101 38-39 0.555841652094017 1101 40-41 0.584455819659695 1101 42-43 0.45758494223128565 1101 44-45 0.5047883922384884 1101 46-47 0.6561714505908469 1101 48-49 0.6181511042104901 1101 50-51 0.8918691873617703 1101 52-53 0.7597932450031308 1101 54-55 0.6964664268900833 1101 56-57 0.6057070084363119 1101 58-59 0.7030403594445218 1101 60-61 0.46901252485057654 1101 62-63 0.5122310803424099 1101 64-65 0.636569580319204 1101 66-67 0.538685998468857 1101 68-69 0.6533760523961973 1101 70-71 0.6282113691368387 1101 72-73 0.49725632541634823 1101 74-75 0.4750564304131082 1101 76-77 0.531684553296234 1101 78-79 0.434074812648781 1101 80-81 0.40140690465739226 1101 82-83 0.40971292899589784 1101 84-85 0.20043642703943831 1101 86-87 0.30180181339982326 1101 88-89 0.10992957822325877 1101 90-91 0.20578153311383218 1101 92-93 0.044683943306271345 1101 94-95 -0.1921688408517923 1101 96-97 -0.22256792980657636 1101 98-99 -0.41885517166580755 1101 100-101 0.003461900520768779 1102 1 0.03235435044447499 1102 2 0.10926113895302692 1102 3 0.08567093916773416 1102 4 0.3446347133276575 1102 5 0.2604158725975907 1102 6 0.24189061302797654 1102 7 0.060552282267202884 1102 8 -0.005791674271918623 1102 9 0.22813769143339613 1102 10-11 0.3237748534002236 1102 12-13 0.438726163709525 1102 14-15 0.33324420597586624 1102 16-17 0.2954480723090782 1102 18-19 0.340239407220011 1102 20-21 0.19138078928864388 1102 22-23 -0.08593256850690523 1102 24-25 0.20113781607749814 1102 26-27 0.11288444045724333 1102 28-29 0.10542903815593263 1102 30-31 0.015283747655871593 1102 32-33 -0.013893480623877963 1102 34-35 -0.041940480558814386 1102 36-37 -0.2695912197364976 1102 38-39 -0.20831527873674816 1102 40-41 -0.46657967457546334 1102 42-43 -0.3822377880748391 1102 44-45 -0.2216707791695498 1102 46-47 -0.06272539164684332 1102 48-49 0.08251251462328213 1102 50-51 -0.019030960162318422 1102 52-53 -0.13351114209376647 1102 54-55 -0.3118798389618789 1102 56-57 -0.14826918819431967 1102 58-59 -0.43186714560973627 1102 60-61 -0.6799890093605612 1102 62-63 -0.6592224200865999 1102 64-65 -0.46796919315712415 1102 66-67 -0.5054948104270096 1102 68-69 -0.48140442076198653 1102 70-71 -0.22674941068666143 1102 72-73 -0.6317050057796401 1102 74-75 -0.4944471918387485 1102 76-77 -0.5195745367327689 1102 78-79 -0.45324212136637954 1102 80-81 -0.3239926438265286 1102 82-83 -0.5162627349361841 1102 84-85 -0.47784079931125234 1102 86-87 -0.3916994608504929 1102 88-89 -0.6028078141681945 1102 90-91 -0.6966905272403245 1102 92-93 -0.7370157975191773 1102 94-95 -0.700126762580485 1102 96-97 -0.7765061151522552 1102 98-99 -0.9556106345287603 1102 100-101 -0.7356706067694354 1103 1 0.11085307845910108 1103 2 0.22863241161884318 1103 3 0.28560640703665996 1103 4 0.29583777662841015 1103 5 0.37734688687148576 1103 6 0.08759187316050543 1103 7 0.2452557527349697 1103 8 0.06836375597521283 1103 9 0.31074540412054574 1103 10-11 0.3196776666938419 1103 12-13 0.4187074845110388 1103 14-15 0.5411013221797987 1103 16-17 0.5304424312350307 1103 18-19 0.42394378201560556 1103 20-21 0.311487925796051 1103 22-23 0.16815282023333822 1103 24-25 0.4153707726807383 1103 26-27 0.2449319418126379 1103 28-29 0.3652597620352367 1103 30-31 0.14501390896425193 1103 32-33 0.3330918372188165 1103 34-35 0.3471347674179768 1103 36-37 0.32545813417394953 1103 38-39 0.2644843731409452 1103 40-41 0.19491280655327614 1103 42-43 0.35758934702779044 1103 44-45 0.4698516413560512 1103 46-47 0.37803070982225506 1103 48-49 0.564002054491624 1103 50-51 -0.010979534199769603 1103 52-53 0.2465724094562063 1103 54-55 0.47910355169676677 1103 56-57 0.6011033246297899 1103 58-59 0.6629201837038821 1103 60-61 0.8127845991928027 1103 62-63 0.5684651566001762 1103 64-65 0.6435877103541685 1103 66-67 0.7222377631538848 1103 68-69 0.6651803698488052 1103 70-71 0.7246177266548379 1103 72-73 0.587896025417475 1103 74-75 0.33912548473015747 1103 76-77 0.3332052287126892 1103 78-79 0.5328162494826572 1103 80-81 0.5214282088316864 1103 82-83 0.42706943872109804 1103 84-85 0.11272779680760081 1103 86-87 0.22144852186191955 1103 88-89 0.2599604386572807 1103 90-91 0.3330938498380398 1103 92-93 0.47613174619914744 1103 94-95 0.28432324367517836 1103 96-97 0.13417559876846852 1103 98-99 0.2816146374829529 1103 100-101 0.27942146126586564 1104 1 0.09860718806993063 1104 2 0.3096702559421942 1104 3 0.32122444095494984 1104 4 0.28576623541154333 1104 5 0.27852826810585896 1104 6 0.2521678361167119 1104 7 0.45029382819248553 1104 8 0.4827191351358735 1104 9 0.36642731157468234 1104 10-11 0.46047846215893884 1104 12-13 0.4206651454073551 1104 14-15 0.4271929214269221 1104 16-17 0.26659353454356705 1104 18-19 0.4397402761778437 1104 20-21 0.34030519411489735 1104 22-23 0.5183863241687874 1104 24-25 0.351636501652564 1104 26-27 0.21730296927540138 1104 28-29 0.2368207486664602 1104 30-31 0.18549598600333894 1104 32-33 0.30614701530573996 1104 34-35 0.24473438072273268 1104 36-37 0.39950839027190455 1104 38-39 0.37645280514887247 1104 40-41 0.1508164989214933 1104 42-43 0.4074101206649843 1104 44-45 0.2358659599874784 1104 46-47 0.40473887892157023 1104 48-49 0.3737736082390839 1104 50-51 0.1695927306098639 1104 52-53 0.2275285127939526 1104 54-55 0.5545946630949885 1104 56-57 0.5265153245181153 1104 58-59 0.46227398193284586 1104 60-61 0.6122792853794863 1104 62-63 0.41061464586510255 1104 64-65 0.44941878374510935 1104 66-67 0.32153179762014616 1104 68-69 0.37487586357669755 1104 70-71 0.5148740258722917 1104 72-73 -0.061678616308341816 1104 74-75 -0.058303861906804144 1104 76-77 0.07580130683343711 1104 78-79 0.07866505398016344 1104 80-81 0.23705268334233054 1104 82-83 0.23680252421281978 1104 84-85 0.39401449990603155 1104 86-87 0.11596970082793234 1104 88-89 0.32285619909841046 1104 90-91 0.19733596676725185 1104 92-93 -0.23565385609038003 1104 94-95 -0.0448958799497845 1104 96-97 0.18309180029927674 1104 98-99 0.154102270831455 1104 100-101 0.20248608598773998 1105 1 0.04626641263294573 1105 2 0.06001287132555433 1105 3 0.12212448720560332 1105 4 0.40564653422255503 1105 5 0.3471160879399804 1105 6 0.24584161509751823 1105 7 0.3571644732941195 1105 8 0.14083414767937796 1105 9 0.4237021362157023 1105 10-11 0.37537396398240475 1105 12-13 0.20233841109507011 1105 14-15 0.37517469874689624 1105 16-17 0.37788780461868754 1105 18-19 0.2397478747764552 1105 20-21 0.013082400490112889 1105 22-23 -0.14563601726183606 1105 24-25 -0.1721263708968408 1105 26-27 -0.09829183622954929 1105 28-29 -0.06675935432556201 1105 30-31 -0.16742945507398588 1105 32-33 -0.09201276694012961 1105 34-35 -0.18507976763525136 1105 36-37 -0.18216161887355753 1105 38-39 -0.2824732414902442 1105 40-41 -0.560943844380084 1105 42-43 -0.3579267395553032 1105 44-45 -0.20178191707649518 1105 46-47 -0.14886496969109686 1105 48-49 -0.002662904888083517 1105 50-51 -0.08938340475064166 1105 52-53 0.14055382892085788 1105 54-55 0.061256230430004166 1105 56-57 0.24899705783599302 1105 58-59 0.16252225451163582 1105 60-61 0.1259154745582336 1105 62-63 -0.11320133933778465 1105 64-65 -0.10514167415574605 1105 66-67 -0.19607446548598517 1105 68-69 -0.13718671786109127 1105 70-71 7.549767671228835E-4 1105 72-73 -0.11245935293044695 1105 74-75 0.038992316347012945 1105 76-77 -0.08494743426098594 1105 78-79 -0.05895370804529776 1105 80-81 0.08300873630456351 1105 82-83 -0.013556373881829842 1105 84-85 -0.14779368742889076 1105 86-87 0.04739389918547232 1105 88-89 -0.10541341778877822 1105 90-91 -0.018600698138033778 1105 92-93 -0.08364234033666307 1105 94-95 0.05039209724511551 1105 96-97 0.23670146416596438 1105 98-99 -0.013841877918089551 1105 100-101 0.16084053192390613 1106 1 0.08467079144455525 1106 2 0.15757030340739675 1106 3 0.027905223234022003 1106 4 0.0395548118272373 1106 5 0.26404416238749917 1106 6 0.2599525671946097 1106 7 0.2904682026688903 1106 8 0.2990375413549131 1106 9 0.3515564847395609 1106 10-11 0.34492693616353876 1106 12-13 0.397260205963768 1106 14-15 0.2626177131377929 1106 16-17 0.13636366733677363 1106 18-19 0.08893107099036257 1106 20-21 0.16340084415853084 1106 22-23 0.2451258583098337 1106 24-25 0.1564162696636444 1106 26-27 0.053438426658431126 1106 28-29 -0.17995612331090882 1106 30-31 -0.09542715754937348 1106 32-33 -0.06238049154609371 1106 34-35 -0.03684671368927894 1106 36-37 -0.02854048357783512 1106 38-39 0.017775093890428195 1106 40-41 -0.05747197578304508 1106 42-43 -0.020201723325413923 1106 44-45 -0.087583601123427 1106 46-47 0.07478505359789267 1106 48-49 0.01367872157592842 1106 50-51 0.050434723973481255 1106 52-53 0.09780370161578844 1106 54-55 0.32652982168783495 1106 56-57 0.5028054703478517 1106 58-59 0.33427935403161513 1106 60-61 0.2727123342861475 1106 62-63 0.36650394038341716 1106 64-65 0.08725139562191231 1106 66-67 0.3587757972089278 1106 68-69 0.2707033002150254 1106 70-71 0.23453293259769126 1106 72-73 0.4105960169299898 1106 74-75 0.44576108449250995 1106 76-77 0.40421196616231825 1106 78-79 0.24484686102644915 1106 80-81 0.01205842510099231 1106 82-83 0.14432652476402907 1106 84-85 0.05884234557630563 1106 86-87 0.0779483217996777 1106 88-89 0.07758248672880086 1106 90-91 0.06772927176728771 1106 92-93 0.04801330126880643 1106 94-95 0.08483855550956676 1106 96-97 0.12567093267032803 1106 98-99 0.16744174328961137 1106 100-101 0.6525050064496192 1107 1 0.3083731451573719 1107 2 0.3190837692883264 1107 3 0.2830725266031209 1107 4 0.3025648204890601 1107 5 0.30929993651918863 1107 6 0.33571598425890414 1107 7 0.5103813841616258 1107 8 0.5034065049902736 1107 9 0.4748427705196505 1107 10-11 0.5658359770042765 1107 12-13 0.41074210876109163 1107 14-15 0.6463840541933337 1107 16-17 0.7161881277251965 1107 18-19 0.9035950873727217 1107 20-21 0.8196821681658051 1107 22-23 0.7146863083240973 1107 24-25 0.7576980192518263 1107 26-27 0.7875910449824417 1107 28-29 0.7602712871043025 1107 30-31 0.6072550036005921 1107 32-33 0.6836868904303088 1107 34-35 0.7221613322934886 1107 36-37 0.58816431847697 1107 38-39 0.6985503521179055 1107 40-41 0.6262773420357775 1107 42-43 0.8224174518487004 1107 44-45 0.6920566077617067 1107 46-47 0.6374796316969977 1107 48-49 0.6638492042848227 1107 50-51 0.7646085712750548 1107 52-53 0.7960402945651666 1107 54-55 0.8849045031279985 1107 56-57 0.7335884891685396 1107 58-59 0.6610238602826541 1107 60-61 0.6592559663439062 1107 62-63 0.7993793152701372 1107 64-65 0.7058312571328713 1107 66-67 0.8004406697599578 1107 68-69 0.7569885796214137 1107 70-71 0.8543564917559472 1107 72-73 0.9728905489662161 1107 74-75 0.8739786014030919 1107 76-77 0.9723234383218156 1107 78-79 1.0895874319057022 1107 80-81 1.041429516640946 1107 82-83 0.9504138339410559 1107 84-85 1.0077025397413415 1107 86-87 1.056398906848269 1107 88-89 1.1775439341044702 1107 90-91 0.8660424692818971 1107 92-93 0.9506499002263809 1107 94-95 1.0826100135497931 1107 96-97 1.2216394296687305 1107 98-99 1.3007086526208447 1107 100-101 1.3414238807129912 1108 1 0.015634779916556596 1108 2 -0.02133094545910552 1108 3 -0.20880121682307973 1108 4 -0.1230707706329639 1108 5 0.00891896150045568 1108 6 -0.0914134647138809 1108 7 0.098131089516734 1108 8 0.06850899519353959 1108 9 0.2943187152815767 1108 10-11 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2316 84-85 -2.161430763064576 2316 86-87 -2.3362066712277 2316 88-89 -2.471669176083317 2316 90-91 -2.4993195021419155 2316 92-93 -2.906477582675805 2316 94-95 -3.0762814825959452 2316 96-97 -3.593574933971855 2316 98-99 -4.0594754403456825 2316 100-101 -4.641277310067167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3290.0 3 1175.0 4 1114.0 5 1230.0 6 1411.0 7 1603.0 8 2046.0 9 2540.0 10 3188.0 11 3943.0 12 4801.0 13 5736.0 14 6645.0 15 7894.0 16 9325.0 17 11163.0 18 9892.0 19 7782.0 20 7584.0 21 8179.0 22 8982.0 23 10238.0 24 11934.0 25 13796.0 26 16039.0 27 18964.0 28 22413.0 29 26903.0 30 32522.0 31 39714.0 32 49547.0 33 63212.0 34 83460.0 35 113575.0 36 155865.0 37 200537.0 38 159113.0 39 26720.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.66449266382365 17.93976977985838 14.539070089493467 22.856667466824504 2 15.600453018404089 19.41202925036199 40.28926282259581 24.698254908638113 3 20.149843501409006 23.26993667266318 29.801196529648077 26.779023296279735 4 12.731485293722496 15.728474955599028 37.56135741719403 33.97868233348444 5 13.844202656698485 36.517337545306304 34.750510820199096 14.887948977796118 6 32.4346563197711 35.0655712830295 17.44607981271541 15.05369258448399 7 28.60250861790253 32.080960177792726 20.79850706811025 18.518024136194498 8 26.356480971866254 33.34827027603296 20.014925386070853 20.280323366029926 9 25.585246890469655 17.081530707045495 21.044188610175134 36.28903379230972 10-11 25.213382349180257 25.477057089937666 27.50760833616167 21.801952224720406 12-13 26.39830609489423 23.06727724692741 27.8443670503382 22.690049607840162 14-15 22.984416478094584 24.71809520673018 26.70770041435303 25.58978790082221 16-17 22.10268495173778 27.895494977036766 27.329886176212366 22.67193389501309 18-19 21.877015192383702 26.78117004236671 29.498169425241127 21.84364534000846 20-21 22.761506747568735 25.46976442660616 29.42266708151944 22.346061744305672 22-23 22.260647782436894 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22.18545815170934 48-49 22.717455773194136 26.14967166473367 28.480057049795963 22.652815512276234 50-51 22.173832797689556 26.456008034223434 28.94785639952065 22.42230276856636 52-53 22.709103969485625 26.639857410107975 28.071301877947697 22.579736742458707 54-55 22.163314148855605 26.26479778312702 28.491982205400422 23.079905862616958 56-57 22.659132136895295 26.308884815363438 28.11346332895605 22.918519718785216 58-59 23.096486287422554 26.0731770720506 28.052770677180366 22.777565963346476 60-61 22.53929660581132 26.188018028283246 29.0144795552804 22.258205810625043 62-63 22.20371293026883 26.313367848710005 28.948725169508048 22.53419405151312 64-65 21.91220853808274 26.83797884499239 28.4379053648316 22.811907252093274 66-67 22.174367390511314 26.469994428409393 28.039181366105492 23.3164568149738 68-69 22.18829313689895 26.233426715342002 28.538024212311345 23.0402559354477 70-71 22.659043938284494 26.14005138625916 28.08735664056915 23.113548034887195 72-73 22.38810108703658 26.039573503862467 28.631131098604484 22.941194310496467 74-75 22.00119058140925 27.097771515816362 28.072765840191742 22.828272062582645 76-77 22.369369076631465 26.46407798537774 27.94203086921202 23.22452206877877 78-79 22.348354506742062 26.522608901412553 28.139932583704812 22.989104008140572 80-81 22.800436274953753 26.375524923095057 27.791078549858106 23.032960252093083 82-83 22.554818874910588 26.258859555972634 28.33420201282353 22.852119556293246 84-85 22.395184777908625 26.583677568680258 28.033148845717577 22.98798880769354 86-87 22.417457959927834 26.708812167574507 27.96945108308536 22.904278789412295 88-89 22.15215403005716 26.638848318659452 27.48648328971101 23.72251436157238 90-91 22.18664926581491 26.341405924695387 27.901156576035785 23.570788233453918 92-93 22.550118982212464 26.425706743943 28.175256552687543 22.848917721156997 94-95 22.205339502963504 26.299868288794148 28.178009427749473 23.31678278049288 96-97 21.731539012441853 26.312022415002488 28.6431636105415 23.31327496201416 98-99 22.025995321639456 26.682515429142946 27.763920462670615 23.527568786546986 100-101 21.90271240215328 26.21212909265857 28.07704643764249 23.80811206754566 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 113.0 1 89.5 2 115.5 3 168.5 4 134.0 5 130.5 6 229.0 7 442.0 8 771.5 9 963.5 10 908.0 11 854.5 12 897.5 13 975.5 14 1091.0 15 1190.5 16 1298.0 17 1402.0 18 1521.5 19 1706.5 20 1893.5 21 2180.5 22 2605.5 23 3134.5 24 3865.0 25 4893.5 26 6272.0 27 7932.0 28 9510.5 29 11196.0 30 13412.5 31 16118.5 32 19097.5 33 20676.5 34 21686.5 35 23550.0 36 25948.5 37 28202.0 38 29653.5 39 30694.0 40 31832.0 41 33536.5 42 34641.5 43 35780.0 44 37356.5 45 39166.5 46 40989.5 47 45433.0 48 54140.0 49 53358.5 50 44435.5 51 40144.5 52 39172.5 53 39132.0 54 38355.0 55 36067.5 56 33011.5 57 29862.0 58 27734.0 59 24916.0 60 20603.0 61 17228.5 62 14431.5 63 11253.0 64 8121.5 65 5907.0 66 4438.5 67 3552.5 68 2885.5 69 2266.5 70 1716.0 71 1234.0 72 932.0 73 764.0 74 598.0 75 473.5 76 351.5 77 233.5 78 165.5 79 121.5 80 84.5 81 55.0 82 40.0 83 29.5 84 20.0 85 14.5 86 11.0 87 6.5 88 4.5 89 2.5 90 2.0 91 1.5 92 1.0 93 1.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012737352329029205 2 0.0042457841096764015 3 0.00710518973456051 4 0.03422621884330977 5 0.0475701117594356 6 0.0659396266829335 7 0.03318643497971555 8 0.030067083388932884 9 0.01854281223409694 10-11 0.05038619305666995 12-13 0.04666030087879066 14-15 0.0207090286165849 16-17 0.024694866760362748 18-19 0.03032702935483144 20-21 0.02278859634377334 22-23 0.016939812111055847 24-25 0.005112270662671586 26-27 0.004202459782026643 28-29 0.0024261623483865153 30-31 0.007841703304606415 32-33 0.0010831081912439802 34-35 0.0028594056248841078 36-37 0.004289108437326161 38-39 0.004505730075574957 40-41 0.0023828380207367565 42-43 6.49864914746388E-4 44-45 0.001732973105990368 46-47 0.0026427839866353113 48-49 5.632162594468697E-4 50-51 0.0019929190718889233 52-53 6.065405870966288E-4 54-55 0.0024261623483865153 56-57 0.0018629460889396459 58-59 0.003119351590782663 60-61 9.098108806449433E-4 62-63 9.531352082947025E-4 64-65 0.0028594056248841078 66-67 0.001473027140091813 68-69 0.003899189488478328 70-71 0.007018541079260991 72-73 0.0026861083142850706 74-75 0.0024261623483865153 76-77 0.0020362433995386826 78-79 0.03110686725252711 80-81 0.01962592042534092 82-83 0.0030760272631329038 84-85 0.006845243768661954 86-87 0.005892108560367252 88-89 0.004462405747925198 90-91 0.01035451430829245 92-93 0.011437622499536431 94-95 0.003985838143777847 96-97 0.0032493245737319405 98-99 0.02278859634377334 100-101 0.04297773302856113 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1154086.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.86725013422628 #Duplication Level Percentage of deduplicated Percentage of total 1 84.39962570126546 56.435708830015905 2 9.603640732720427 12.84338094148122 3 2.551583382098536 5.118520927473536 4 1.0824373368513798 2.895184326314679 5 0.6000270192039305 2.006107839020171 6 0.3944031918558787 1.582359413013857 7 0.25267299401032767 1.182688380485671 8 0.18383077385329305 0.9833806670093225 9 0.14807156816843073 0.8911024727837037 >10 0.7271925679499682 8.426504891909973 >50 0.03527516394871919 1.6097382800967164 >100 0.017483798421671016 2.407723601881904 >500 0.002201658071850236 1.0205728267589884 >1k 0.0015541115801295784 2.597026601754375 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4500 0.38991894884783285 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4323 0.3745821368598181 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3899 0.3378431070128223 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3085 0.2673111015990143 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2769 0.23993012652436646 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2692 0.23325818006630356 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1844 0.15978012037231193 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1783 0.1544945523990413 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1512 0.13101276681287183 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1374 0.11905525238153829 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1177 0.10198546728753316 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 8.664865529951841E-5 0.0 0.0 0.0 0.0 7 2.5994596589855524E-4 0.0 0.0 0.0 0.0 8 2.5994596589855524E-4 0.0 0.0 0.0 0.0 9 2.5994596589855524E-4 0.0 0.0 0.0 0.0 10-11 2.5994596589855524E-4 0.0 0.0 0.0 0.0 12-13 3.4659462119807365E-4 0.0 0.0 0.0 0.0 14-15 3.4659462119807365E-4 0.0 0.0 0.0 0.0 16-17 3.4659462119807365E-4 0.0 0.0 0.0 0.0 18-19 3.4659462119807365E-4 0.0 0.0 1.7329731059903682E-4 0.0 20-21 3.4659462119807365E-4 0.0 0.0 3.0327029354831447E-4 0.0 22-23 4.3324327649759206E-4 0.0 0.0 4.3324327649759206E-4 0.0 24-25 4.3324327649759206E-4 0.0 0.0 6.49864914746388E-4 0.0 26-27 4.3324327649759206E-4 0.0 0.0 9.098108806449433E-4 0.0 28-29 6.49864914746388E-4 0.0 0.0 0.001906270416589405 0.0 30-31 6.931892423961473E-4 0.0 0.0 0.0040291624714276055 0.0 32-33 6.931892423961473E-4 0.0 0.0 0.006282027509215084 0.0 34-35 7.365135700459065E-4 0.0 0.0 0.009011460151149914 8.664865529951841E-5 36-37 8.664865529951841E-4 0.0 0.0 0.012390757707831133 8.664865529951841E-5 38-39 0.001039783863594221 0.0 0.0 0.020405758323036587 8.664865529951841E-5 40-41 0.001039783863594221 0.0 0.0 0.032883164686167234 8.664865529951841E-5 42-43 0.00108310819124398 0.0 0.0 0.054718625821645876 8.664865529951841E-5 44-45 0.0011264325188937393 0.0 0.0 0.07395462729813895 8.664865529951841E-5 46-47 0.0012130811741932577 0.0 0.0 0.11758222524144649 8.664865529951841E-5 48-49 0.0012130811741932577 0.0 0.0 0.1566607687815293 8.664865529951841E-5 50-51 0.0012130811741932577 0.0 0.0 0.1837384735626288 8.664865529951841E-5 52-53 0.0012130811741932577 0.0 0.0 0.2144987461939578 8.664865529951841E-5 54-55 0.0012130811741932577 0.0 0.0 0.2508478570921058 8.664865529951841E-5 56-57 0.0012130811741932577 0.0 0.0 0.3299580793805661 8.664865529951841E-5 58-59 0.0012130811741932577 0.0 0.0 0.4232786811381474 8.664865529951841E-5 60-61 0.0012130811741932577 0.0 0.0 0.519805283141811 8.664865529951841E-5 62-63 0.0012130811741932577 0.0 0.0 0.6196245340468561 8.664865529951841E-5 64-65 0.0012130811741932577 0.0 0.0 0.746304868094752 8.664865529951841E-5 66-67 0.0012130811741932577 0.0 0.0 0.9140566647546197 8.664865529951841E-5 68-69 0.0012130811741932577 0.0 0.0 1.100004679027386 8.664865529951841E-5 70-71 0.001299729829492776 0.0 0.0 1.2301509592872628 8.664865529951841E-5 72-73 0.0014297028124420539 0.0 0.0 1.3509825091024412 8.664865529951841E-5 74-75 0.001473027140091813 0.0 0.0 1.496378952695033 8.664865529951841E-5 76-77 0.001473027140091813 0.0 0.0 1.6773446692880774 8.664865529951841E-5 78-79 0.001473027140091813 0.0 0.0 1.858050439915223 8.664865529951841E-5 80-81 0.0018196217612898866 0.0 0.0 2.1224154872340537 8.664865529951841E-5 82-83 0.0019929190718889233 0.0 0.0 2.4325310245510297 2.5994596589855524E-4 84-85 0.002122892054838201 0.0 0.0 2.8271723251126866 2.5994596589855524E-4 86-87 0.002296189365437238 0.0 0.0 3.263621601856361 3.0327029354831447E-4 88-89 0.002339513693086997 0.0 0.0 3.7782712900078503 3.4659462119807365E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTACG 100 0.0016509427 23.756685 1 GAGTACT 3880 0.0 23.511261 12-13 GTACATG 3940 0.0 22.91254 1 GTGTAGC 730 0.0 22.780384 1 TACATGG 3865 0.0 22.619562 2 GTACTTT 4115 0.0 22.10941 14-15 ACATGGG 3930 0.0 21.640951 3 ACGTTCG 110 0.0028712312 21.59886 7 CGTTCGA 110 0.0028712312 21.59886 8 TACCTGG 865 0.0 20.872925 2 CATGGGG 2260 0.0 20.392908 4 AGTACTT 4225 0.0 20.017035 12-13 AGAGTAC 6080 0.0 19.88927 10-11 GTATAGC 435 0.0 19.660707 1 CCTACAC 465 0.0 19.414064 3 GTATCAA 9970 0.0 19.014881 1 TAGCGCG 125 0.0060213585 19.00535 4 GTGTAGG 425 0.0 19.005348 1 GTACTAG 280 3.4769982E-8 18.665968 1 TCGTTAC 105 1.9579238E-5 18.094448 22-23 >>END_MODULE