FastQCFastQC Report
Thu 26 May 2016
SRR936642_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936642_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154086
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT81570.7067930812781716No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36290.3144479700819523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32230.27926861603034786No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27860.2414031536644583No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22640.19617255559810967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17830.1544945523990413No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16090.13941768637692512No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15990.13855119982392994No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC15950.13820460520273184No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14950.12953973967278No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13980.12113482010872674No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG13800.11957514431333541No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC13090.1134230897870696No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA12330.1068377919843062No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC11960.10363179173822402No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA11850.1026786565299293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGT500.001616767437.995466
GTAAGGT6250.037.2339364
GGTAAGG6700.032.608043
TAAGGTG7800.032.2717865
AGGTAAG6500.032.1486132
TATACCG600.003953270631.6628845
GGTGTGC12250.023.6502348
AAGGTAA8750.023.0408731
AAGGTGT10800.022.4278766
CTCTACG1302.7396932E-422.1546841
GAGTACT44900.022.10568812-13
AGGTGTG12600.021.108597
GTACTTT47550.020.77384614-15
GTACATG39850.020.7184751
GGACCAT5850.020.2967196
TACATGG39650.020.003052
ACATGGG38500.019.9846253
GTGTGCA14050.019.9442379
AGTACTT48350.019.10412612-13
AGAGTAC68100.019.0038810-11
Produced by FastQC (version 0.11.5)