##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936642_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1154086 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.483124307893867 31.0 31.0 34.0 30.0 34.0 2 31.721510355380794 31.0 31.0 34.0 30.0 34.0 3 31.988897707796472 33.0 31.0 34.0 30.0 34.0 4 35.77652705257667 37.0 35.0 37.0 35.0 37.0 5 35.583332611261206 37.0 35.0 37.0 33.0 37.0 6 35.68068410846332 37.0 35.0 37.0 33.0 37.0 7 35.61814544150089 37.0 35.0 37.0 33.0 37.0 8 35.592759984957794 37.0 35.0 37.0 33.0 37.0 9 37.2349928861454 39.0 37.0 39.0 33.0 39.0 10-11 37.115849685378734 39.0 37.0 39.0 33.0 39.0 12-13 36.99251658888505 39.0 37.0 39.0 33.0 39.0 14-15 38.12005041218765 40.0 37.0 41.0 33.0 41.0 16-17 38.048529745616875 40.0 37.0 41.0 33.0 41.0 18-19 38.09262308008242 40.0 37.0 41.0 33.0 41.0 20-21 38.14474267948836 40.0 37.0 41.0 33.0 41.0 22-23 38.05774526335126 40.0 37.0 41.0 33.0 41.0 24-25 37.94486416090309 40.0 37.0 41.0 33.0 41.0 26-27 37.725216751611235 40.0 37.0 41.0 32.0 41.0 28-29 37.647346038336835 39.5 37.0 41.0 32.0 41.0 30-31 37.65894872652471 40.0 37.0 41.0 32.5 41.0 32-33 37.486600651944485 40.0 36.0 41.0 32.0 41.0 34-35 37.32254918610918 39.0 36.0 41.0 31.5 41.0 36-37 37.09553620787359 39.0 36.0 41.0 31.0 41.0 38-39 36.922814244345744 39.0 36.0 41.0 31.0 41.0 40-41 36.70731253996669 39.0 35.0 41.0 30.5 41.0 42-43 36.47777765261861 39.0 35.0 40.5 30.0 41.0 44-45 36.22190763946534 39.0 35.0 40.0 30.0 41.0 46-47 35.851141076141644 38.5 35.0 40.0 28.5 41.0 48-49 35.384724795205905 38.0 34.5 40.0 27.0 41.0 50-51 35.19878977823143 38.0 34.0 40.0 27.0 41.0 52-53 34.94673403888445 38.0 34.0 40.0 26.0 41.0 54-55 34.67599295026541 38.0 34.0 40.0 26.0 41.0 56-57 34.37547765071234 38.0 33.5 40.0 25.0 41.0 58-59 33.98355408522415 37.0 33.0 40.0 24.0 41.0 60-61 33.58503439085129 37.0 32.5 39.5 23.0 41.0 62-63 33.288907412445866 36.0 32.0 39.0 23.0 40.5 64-65 32.85779525962536 36.0 32.0 39.0 22.0 40.0 66-67 32.44752687408044 35.0 31.0 38.5 21.5 40.0 68-69 31.96588035900271 35.0 31.0 38.0 20.0 40.0 70-71 31.47399500557151 35.0 30.5 37.0 18.5 39.5 72-73 31.044177383661182 34.0 30.0 37.0 18.0 39.0 74-75 30.67984664920985 34.0 30.0 36.0 16.0 39.0 76-77 29.2670255942798 32.5 28.0 35.0 13.0 37.0 78-79 30.090866278596224 34.0 29.5 35.0 13.0 37.0 80-81 29.92739752496781 34.0 29.5 35.0 9.0 37.0 82-83 29.57744916756637 34.0 29.0 35.0 7.0 36.5 84-85 29.147454349155957 34.0 29.0 35.0 4.5 36.0 86-87 28.78087031642356 34.0 29.0 35.0 2.0 36.0 88-89 28.365043852884448 33.0 28.5 35.0 2.0 35.0 90-91 27.907732179404306 33.0 27.0 35.0 2.0 35.0 92-93 27.448642908760696 33.0 26.5 35.0 2.0 35.0 94-95 26.8858438625891 32.5 25.5 34.0 2.0 35.0 96-97 26.18433201685143 32.0 25.0 34.0 2.0 35.0 98-99 25.335502293589904 32.0 22.0 34.0 2.0 35.0 100-101 22.577246842956242 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4432344203749139 1101 2 0.4148691335683772 1101 3 0.26540139293732423 1101 4 0.1461016757637168 1101 5 0.2640272470719296 1101 6 0.20482585464742442 1101 7 0.22343309236949693 1101 8 0.2618991187716446 1101 9 0.3708831145814102 1101 10-11 0.28571605988812365 1101 12-13 0.2582874324093538 1101 14-15 0.38553938387301656 1101 16-17 0.3419859858401182 1101 18-19 0.4087081334242697 1101 20-21 0.1617981905738901 1101 22-23 0.14915821839458232 1101 24-25 0.19371503788737243 1101 26-27 0.21334851083535966 1101 28-29 0.13120862530626454 1101 30-31 0.08298283161825282 1101 32-33 0.1372904348199384 1101 34-35 0.32167328796264627 1101 36-37 0.2259519629375859 1101 38-39 0.32882764960203303 1101 40-41 0.41018889607733655 1101 42-43 0.5305706857690708 1101 44-45 0.5236915664563853 1101 46-47 0.3772886110758549 1101 48-49 0.48993324053009246 1101 50-51 0.2534910189466757 1101 52-53 0.3676850509294596 1101 54-55 0.49963381238899984 1101 56-57 0.2528550861742289 1101 58-59 0.24550072550078283 1101 60-61 0.3141566977016552 1101 62-63 0.23181538963603288 1101 64-65 0.2637778910019932 1101 66-67 0.07601213174787347 1101 68-69 -0.3938750859512652 1101 70-71 -0.22391494046404148 1101 72-73 -0.015506764568804954 1101 74-75 -0.03438103596103659 1101 76-77 0.36503481982009234 1101 78-79 0.21928980655803443 1101 80-81 0.08258257175732808 1101 82-83 0.3670163889485174 1101 84-85 0.35287554896306617 1101 86-87 0.25670341399254326 1101 88-89 0.17387239453087133 1101 90-91 0.12655690548325182 1101 92-93 0.22666406295949315 1101 94-95 -0.07339850284177984 1101 96-97 -0.10978736586040938 1101 98-99 -0.2700751609235539 1101 100-101 -0.3074062394899997 1102 1 0.4370587536908843 1102 2 0.5385593782067453 1102 3 0.18642342719711635 1102 4 0.10667584027714838 1102 5 0.18561048220330179 1102 6 0.1772805728135296 1102 7 0.17077259727435745 1102 8 -0.05640251637702676 1102 9 0.14646984468703295 1102 10-11 0.19686449702723507 1102 12-13 0.11194889953191733 1102 14-15 0.18488242994553872 1102 16-17 0.291482698736246 1102 18-19 0.1669826520544646 1102 20-21 0.2563385464204515 1102 22-23 0.11555926472461664 1102 24-25 0.19701418154005523 1102 26-27 -0.06337044939996872 1102 28-29 -0.07414691166271581 1102 30-31 0.07209666497402623 1102 32-33 0.2738710998282343 1102 34-35 0.3154556049261359 1102 36-37 0.35155972579041617 1102 38-39 0.30956401197857986 1102 40-41 0.35337357342613984 1102 42-43 0.3217370056575035 1102 44-45 0.18289386020524034 1102 46-47 0.2514282586888896 1102 48-49 0.3301934568921965 1102 50-51 0.2429815252166989 1102 52-53 0.19914566711032933 1102 54-55 0.13051499604124928 1102 56-57 0.13591202889734433 1102 58-59 0.02622327899423027 1102 60-61 0.19057886523436451 1102 62-63 0.21108461961143377 1102 64-65 0.12282371683708249 1102 66-67 0.07448013199554993 1102 68-69 -0.06907037599186339 1102 70-71 -0.13502774970193698 1102 72-73 -0.13547647348148217 1102 74-75 -0.6927831761904919 1102 76-77 -0.288588390307865 1102 78-79 -0.03553728263752021 1102 80-81 -0.02776637527847825 1102 82-83 0.12716948081149937 1102 84-85 0.20881683319901967 1102 86-87 0.1389331751332641 1102 88-89 -0.06624626849405146 1102 90-91 -0.20995488710634191 1102 92-93 0.16942599683214254 1102 94-95 -0.11420928084702808 1102 96-97 -0.18692852901064327 1102 98-99 -0.5993084190042062 1102 100-101 -0.5413964120862964 1103 1 0.432095925515366 1103 2 0.49229927859898837 1103 3 0.27331901464802755 1103 4 0.11805950574608914 1103 5 0.09852722477936027 1103 6 0.08128957658229297 1103 7 -0.006647961257826296 1103 8 0.14017793916713828 1103 9 -0.06105418475191016 1103 10-11 0.14281170359809892 1103 12-13 0.2186782399213527 1103 14-15 0.31358260137273675 1103 16-17 0.10616271594481219 1103 18-19 0.14814870815352066 1103 20-21 0.12730473948566612 1103 22-23 0.10721206649463966 1103 24-25 0.031206114145682307 1103 26-27 0.029403268901916135 1103 28-29 0.2323192517703916 1103 30-31 0.13007735801399178 1103 32-33 -0.16832106105432132 1103 34-35 -0.04973754149813914 1103 36-37 -0.16229163520100798 1103 38-39 -0.0989391937757631 1103 40-41 -0.011808872517221403 1103 42-43 -0.17342994379452392 1103 44-45 -0.08169144402864248 1103 46-47 -0.024317098399876613 1103 48-49 0.08642003930126663 1103 50-51 -0.24358086572070903 1103 52-53 -0.23881513949741162 1103 54-55 -0.31252552091819297 1103 56-57 -0.1393493372083583 1103 58-59 -0.19245449275957327 1103 60-61 -0.03191513700889459 1103 62-63 -0.13141527612746984 1103 64-65 -0.25144645549004707 1103 66-67 -0.3802568337212051 1103 68-69 -0.24433519917602098 1103 70-71 -0.3527616841645127 1103 72-73 -0.2807055954713533 1103 74-75 -0.22390895458188353 1103 76-77 -0.24547615268914313 1103 78-79 -0.0058780188745082285 1103 80-81 -0.043199212475325766 1103 82-83 -0.02123768401103021 1103 84-85 0.1589610976315008 1103 86-87 -0.045224570807686604 1103 88-89 -0.2316265910230051 1103 90-91 -0.4007847944566372 1103 92-93 -0.12110522991488892 1103 94-95 -0.041280015964581196 1103 96-97 -0.4860248048042912 1103 98-99 -0.6361614240284936 1103 100-101 -0.405013844102573 1104 1 0.3609640628272359 1104 2 0.3841937411828482 1104 3 0.20212287588702438 1104 4 0.1279563978356535 1104 5 -0.00553054317882129 1104 6 0.08974480180500422 1104 7 0.05474445325662458 1104 8 0.04246207706593452 1104 9 0.03588679358185942 1104 10-11 -0.013313416594904481 1104 12-13 5.741048922587311E-4 1104 14-15 0.20608265493613231 1104 16-17 -0.010162530073479559 1104 18-19 0.04538409037865421 1104 20-21 -0.05953460474257355 1104 22-23 0.05170497048111855 1104 24-25 0.17198873051945895 1104 26-27 0.1497840754173012 1104 28-29 -0.015159048562473743 1104 30-31 -0.07699612641788178 1104 32-33 0.051580044688904536 1104 34-35 0.1863981420454266 1104 36-37 0.13626488002300619 1104 38-39 0.22912571986381636 1104 40-41 0.21604363791790604 1104 42-43 0.25210233026047746 1104 44-45 0.147114981760744 1104 46-47 0.2212392499660467 1104 48-49 0.2274693364521383 1104 50-51 0.3307040954312228 1104 52-53 0.23196976224785004 1104 54-55 0.5581396585256755 1104 56-57 0.45258687236020734 1104 58-59 0.26556414210753587 1104 60-61 0.27633261933414843 1104 62-63 0.06313459983269354 1104 64-65 0.1743005590811464 1104 66-67 0.26034415405271005 1104 68-69 0.32956670255170906 1104 70-71 0.27746261335969535 1104 72-73 0.3541702770438704 1104 74-75 0.07233686828494612 1104 76-77 0.3442749950283108 1104 78-79 0.34546018948514856 1104 80-81 0.3232631882944297 1104 82-83 0.2945467665032133 1104 84-85 0.4392404842949311 1104 86-87 0.36478811560104063 1104 88-89 0.2737613994797172 1104 90-91 0.203458335307662 1104 92-93 0.2232176346424417 1104 94-95 0.3664792746872756 1104 96-97 0.13874430450463748 1104 98-99 0.26590927186062885 1104 100-101 0.36341959218068354 1105 1 0.3732299843143281 1105 2 0.44005235762536543 1105 3 0.16618150450280567 1105 4 0.039801411235934836 1105 5 -0.012656555469163777 1105 6 0.06631010342478305 1105 7 0.15930427147953452 1105 8 0.07955874558443554 1105 9 0.06325337905465744 1105 10-11 0.04942638994321413 1105 12-13 0.024341048119481457 1105 14-15 0.23076802061674329 1105 16-17 0.09742994312691877 1105 18-19 0.04448815357417857 1105 20-21 -0.08579028285353019 1105 22-23 0.013062413599215006 1105 24-25 -0.008364201124550164 1105 26-27 0.1010797721680845 1105 28-29 0.10246628857687057 1105 30-31 0.08505620078862819 1105 32-33 0.10525328866633998 1105 34-35 0.08402970538343624 1105 36-37 0.24528635498077023 1105 38-39 0.1385358864010584 1105 40-41 0.20940823922602192 1105 42-43 0.37490072354454895 1105 44-45 0.2528665393765337 1105 46-47 0.435271419175983 1105 48-49 0.6616641156721457 1105 50-51 0.5494171564530106 1105 52-53 0.4692584892228311 1105 54-55 0.5540972816064951 1105 56-57 0.47604618864915693 1105 58-59 0.491357274912815 1105 60-61 0.2757018581135142 1105 62-63 0.14244865572813836 1105 64-65 0.3001641727652853 1105 66-67 0.2534040360495666 1105 68-69 0.20144793335410327 1105 70-71 0.21324728303194007 1105 72-73 0.1049114311702013 1105 74-75 0.0982403358433892 1105 76-77 -0.06006872835478916 1105 78-79 0.16496362106655127 1105 80-81 0.3100102243563718 1105 82-83 0.4980480921872896 1105 84-85 0.44140414647829473 1105 86-87 0.13456259617285937 1105 88-89 0.3033734483759254 1105 90-91 -0.00538895340768164 1105 92-93 0.37359758395668763 1105 94-95 0.3924746232594174 1105 96-97 0.3512280654077564 1105 98-99 0.37415518577238416 1105 100-101 0.49209585039812254 1106 1 0.20586352189888402 1106 2 0.38836674201184707 1106 3 0.025604372112773888 1106 4 -0.029967557218952834 1106 5 -0.05517958501600617 1106 6 -0.12509059017666146 1106 7 -0.028166979295761507 1106 8 0.03717134721732407 1106 9 -0.049049237758296726 1106 10-11 -0.06592454970090955 1106 12-13 -0.11750816958787169 1106 14-15 0.032510712340588555 1106 16-17 0.0696214485606177 1106 18-19 -0.050308387589396375 1106 20-21 -0.03064076275138916 1106 22-23 0.03213355722105149 1106 24-25 -0.049806766629345134 1106 26-27 0.055227262629259144 1106 28-29 0.005317922063774461 1106 30-31 0.00963705941605042 1106 32-33 0.04548000028596988 1106 34-35 -0.32102436608599305 1106 36-37 -0.08481338944374528 1106 38-39 0.004542899643197984 1106 40-41 -0.045941107596419783 1106 42-43 -0.18898831953161732 1106 44-45 -0.04734318214514133 1106 46-47 0.057755303923265444 1106 48-49 -0.1338113053999237 1106 50-51 -0.23187298084339858 1106 52-53 -0.155463551326541 1106 54-55 -0.3059549919051179 1106 56-57 -0.32710108219568923 1106 58-59 -0.1939672866388804 1106 60-61 -0.2971513923287361 1106 62-63 -0.6348193654805172 1106 64-65 -0.27804240655360246 1106 66-67 -0.1163432121096335 1106 68-69 0.001313750194086083 1106 70-71 -0.11545391568704133 1106 72-73 -0.1252952253764157 1106 74-75 -0.08580538296566331 1106 76-77 -0.05996712563830542 1106 78-79 -0.15460105594058504 1106 80-81 -0.22139828855151933 1106 82-83 -0.05867397732248136 1106 84-85 0.08983292442205837 1106 86-87 0.16108911062047682 1106 88-89 0.16203224425099805 1106 90-91 -0.11840037586984664 1106 92-93 0.1155669873001628 1106 94-95 0.0845696423129425 1106 96-97 -0.07020190232553958 1106 98-99 -0.05187924330964577 1106 100-101 -0.07104408381351135 1107 1 0.45468015660526007 1107 2 0.54488138449884 1107 3 0.368323183290034 1107 4 0.10212875424619483 1107 5 0.09309981636688036 1107 6 0.18941270682374522 1107 7 0.06488103123862032 1107 8 0.06620497812350834 1107 9 0.1274256504641258 1107 10-11 0.2101352719556573 1107 12-13 0.28604267731815725 1107 14-15 0.3220138841701399 1107 16-17 0.30725882357752 1107 18-19 0.15788780020694304 1107 20-21 0.19444848407052007 1107 22-23 0.3286037390192078 1107 24-25 0.354574213408398 1107 26-27 0.26889992581450883 1107 28-29 0.3372505100463741 1107 30-31 0.26182279294231137 1107 32-33 0.4563948991228841 1107 34-35 0.4703128091582087 1107 36-37 0.46392013181277036 1107 38-39 0.5596285916127357 1107 40-41 0.5325830779168115 1107 42-43 0.4628764759141646 1107 44-45 0.39769829947726976 1107 46-47 0.5922904443900023 1107 48-49 0.7571708852903711 1107 50-51 0.5741186977979496 1107 52-53 0.40505205247264087 1107 54-55 0.456687014433534 1107 56-57 0.8035893101899134 1107 58-59 0.33989743871122613 1107 60-61 0.4876733935039326 1107 62-63 0.5036446378840722 1107 64-65 0.3433247596282314 1107 66-67 0.36625703723071723 1107 68-69 0.5555863322686179 1107 70-71 0.522726700854534 1107 72-73 0.5861839436893561 1107 74-75 0.8736617897250589 1107 76-77 0.5262866107118143 1107 78-79 0.7916731082935975 1107 80-81 0.794489398140815 1107 82-83 0.9635508595798861 1107 84-85 0.7728958052543966 1107 86-87 0.9938257961962869 1107 88-89 0.8238531297597156 1107 90-91 0.8360594618085848 1107 92-93 0.7881695898526466 1107 94-95 0.8833329542337154 1107 96-97 0.720784794515648 1107 98-99 0.9665138632808521 1107 100-101 1.4371335786958142 1108 1 0.269197483705657 1108 2 0.21057250421545604 1108 3 -0.012816902610921943 1108 4 0.04980906536479068 1108 5 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-1.427562341517497 2316 92-93 -1.7612853480972213 2316 94-95 -1.9156461358946046 2316 96-97 -2.060025033060736 2316 98-99 -2.4532488036615483 2316 100-101 -2.7103217433086186 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 14.0 5 39.0 6 104.0 7 243.0 8 566.0 9 1035.0 10 1607.0 11 2405.0 12 3468.0 13 4637.0 14 5842.0 15 7512.0 16 9179.0 17 11021.0 18 10202.0 19 7757.0 20 7186.0 21 7459.0 22 8159.0 23 8861.0 24 10373.0 25 12743.0 26 15812.0 27 19218.0 28 23510.0 29 29176.0 30 35880.0 31 44142.0 32 54435.0 33 69077.0 34 90564.0 35 122222.0 36 171070.0 37 216798.0 38 133461.0 39 8303.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.41117652776793 19.33469420996908 14.706224915952292 22.547904346310702 2 15.907154896379005 21.028974015676386 38.61867560974553 24.44519547819908 3 20.746694164745843 23.710507616852393 29.431898927228296 26.11089929117346 4 13.494725129596988 16.777155573580412 36.68768988926845 33.04042940755415 5 13.776962895312828 37.62414586088039 33.87416535682783 14.724725886978959 6 32.613514070875134 36.24695213354984 16.356493363579492 14.783040431995536 7 29.016988335358025 32.80804030202255 19.987765209871707 18.187206152747713 8 26.706588590451663 34.15438710806647 19.053952651708798 20.085071649773067 9 25.96181675438946 17.532369624631936 19.92150875119366 36.58430486978494 10-11 25.531949000135608 26.24572008129408 26.58941331266466 21.63291760590565 12-13 26.74719810135055 23.628316321999232 27.07568386516227 22.548801711487947 14-15 22.921385408019855 25.636261075864365 25.88819204114771 25.55416147496807 16-17 22.27221368251586 28.488908105635108 26.6372263418844 22.60165186996463 18-19 22.219141381144905 27.526544815551006 28.761418126552098 21.492895676751992 20-21 23.044869725856536 25.974808625530777 28.825940539067513 22.154381109545177 22-23 22.370248098589567 26.092355149459202 28.966988785415193 22.570407966536045 24-25 23.05192160722858 26.121666842852264 28.86751940496635 21.958892144952802 26-27 22.58389867995049 26.04100822686014 29.296486265532316 22.078606827657048 28-29 21.99173198531132 27.288131040494385 28.814013860318905 21.906123113875395 30-31 22.408555341629654 26.334865859216734 28.660212497162256 22.59636630199136 32-33 22.34329013275588 26.47305910433431 28.77012882186033 22.413521941049474 34-35 23.027369550232677 26.31667193062493 28.745024692946597 21.910933826195798 36-37 23.209686231235118 25.93807636667546 28.942537609922613 21.909699792166805 38-39 22.58718154452961 26.62964458454569 28.84685370067742 21.936320170247278 40-41 22.59073454591459 26.63245062235738 28.634765101194542 22.142049730533486 42-43 22.42277265388925 26.87772484906362 27.874593899457782 22.82490859758935 44-45 22.861805905769035 26.42484173239127 28.107166190954008 22.606186170885692 46-47 23.56209192802769 27.096368890564143 27.46554540294873 21.87599377845943 48-49 23.351323854512493 27.096359345928434 27.24298729311117 22.309329506447902 50-51 22.62500525309521 27.321727278359457 27.994730008235987 22.058537460309346 52-53 23.207575699152166 27.6471253406998 26.98437730842934 22.160921651718695 54-55 22.512390979098576 27.480134787508625 27.247112021210356 22.760362212182436 56-57 23.105171921515005 27.331710324562756 27.12603967229607 22.437078081626165 58-59 23.55798079401768 26.99148879939865 26.828760016203557 22.62177039038011 60-61 22.76600123482198 27.375353386553435 27.841076244841368 22.017569133783212 62-63 22.445877777166395 27.337252059410854 27.88346765231278 22.333402511109966 64-65 22.321844101076124 28.07244014237392 27.213802678480892 22.39191307806906 66-67 22.660702734763746 27.29075077867178 27.035085613819522 23.01346087274495 68-69 22.599582093354435 27.242262380040298 27.132608459251934 23.025547067353326 70-71 23.23032678288733 27.28264081263772 26.88569747562754 22.601334928847407 72-73 22.879534107510185 27.054656238789832 27.494614786073136 22.571194867626847 74-75 22.42033955875039 28.170257675771133 26.759704216150272 22.649698549328214 76-77 22.768988815645532 27.3342763746488 26.93425644513861 22.962478364567062 78-79 22.875500943066353 27.724070874913398 26.700108367054508 22.70031981496575 80-81 23.294188813197835 27.423061106963292 26.5720675300387 22.710682549800175 82-83 22.887658224420104 27.46498188594973 27.004992033804225 22.64236785582594 84-85 22.59150938491135 27.892814433525707 26.914331270905905 22.60134491065703 86-87 22.556296825557077 27.897513801501884 26.514005583428407 23.032183789512636 88-89 22.175769570966164 28.084231893830818 26.09440486555287 23.645593669650143 90-91 22.471502563729214 27.651873654432187 26.408274310358575 23.46834947148003 92-93 22.70355984671408 27.633144939246694 26.76905946653207 22.894235747507157 94-95 22.344089990927078 27.719472470564995 26.75732658106749 23.179110957440447 96-97 21.965481218231442 27.69202171841711 27.331737945658897 23.010759117692555 98-99 22.089880892803524 27.963478819249012 26.228150790256688 23.71848949769078 100-101 21.48686240185798 28.01779121964747 26.399206197895904 24.096140180598642 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 221.0 1 158.5 2 114.5 3 141.5 4 141.0 5 137.0 6 213.5 7 411.5 8 657.5 9 843.5 10 855.0 11 803.0 12 824.0 13 931.0 14 1048.0 15 1174.5 16 1345.0 17 1478.5 18 1636.0 19 1771.0 20 1927.0 21 2245.5 22 2658.5 23 3171.5 24 3879.0 25 4827.0 26 6104.0 27 7833.5 28 9458.0 29 11212.5 30 13513.5 31 15888.0 32 18692.0 33 20228.5 34 21521.5 35 23548.5 36 25815.5 37 28102.0 38 29719.5 39 30862.0 40 31646.5 41 33136.0 42 34156.0 43 35637.0 44 37183.5 45 38617.0 46 40269.0 47 45103.5 48 55097.5 49 53761.5 50 43915.5 51 39831.5 52 38850.0 53 38812.0 54 38758.0 55 36938.5 56 33065.0 57 30038.5 58 28474.5 59 25608.5 60 21036.0 61 17344.5 62 14638.5 63 11304.0 64 7839.5 65 5737.0 66 4565.0 67 3800.5 68 3023.0 69 2458.0 70 1889.0 71 1310.5 72 1014.5 73 797.0 74 630.5 75 526.0 76 415.0 77 281.0 78 190.5 79 136.0 80 93.5 81 64.0 82 37.0 83 19.5 84 15.0 85 6.5 86 4.0 87 3.5 88 2.0 89 1.0 90 0.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0549473782716365 2 0.010484487291241728 3 0.0057188112497682155 4 0.010744433257140282 5 0.0 6 0.0 7 0.0 8 0.0 9 0.006238703181565326 10-11 3.899189488478329E-4 12-13 2.16621638248796E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.3324327649759206E-5 22-23 7.365135700459065E-4 24-25 0.0 26-27 4.3324327649759206E-5 28-29 0.0 30-31 0.0 32-33 0.004375757092625679 34-35 9.964595359444617E-4 36-37 0.0030760272631329038 38-39 0.0 40-41 0.004029162471427606 42-43 0.010657784601840765 44-45 0.0024261623483865153 46-47 0.0026861083142850706 48-49 0.012564055018430168 50-51 3.899189488478329E-4 52-53 0.0023828380207367565 54-55 0.010527811618891486 56-57 0.00489564902442279 58-59 0.0016030001230410906 60-61 0.006368676164514603 62-63 0.004722351713823753 64-65 0.019322650131792605 66-67 0.0032493245737319405 68-69 4.3324327649759206E-5 70-71 0.001473027140091813 72-73 0.0 74-75 0.0 76-77 0.0016030001230410906 78-79 0.01191419010368378 80-81 0.01728640673225392 82-83 0.012780676656678964 84-85 0.009184757461748952 86-87 0.011740892793084745 88-89 0.029677164440085054 90-91 0.025821299279256485 92-93 0.0029027299525338666 94-95 0.008924811495850397 96-97 0.011654244137785225 98-99 0.010874406240089559 100-101 0.012650703673729686 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1154086.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.3198220495036 #Duplication Level Percentage of deduplicated Percentage of total 1 83.20481718026403 53.51719034696063 2 10.002402500302205 12.867054977738954 3 2.874876541759202 5.547346427407326 4 1.2712124394502713 3.2705663157022733 5 0.7025156505493089 2.25928408151614 6 0.42104439189185205 1.624890021685523 7 0.2981737545700204 1.3424937983643253 8 0.2055921284201961 1.0578919291812565 9 0.16783804949250897 0.9715782128844559 >10 0.7842278014997444 9.01257224321426 >50 0.041558555006255216 1.8308367468417452 >100 0.021428384226953336 2.6072683532944967 >500 0.0021563112837397697 0.984204161336998 >1k 0.002021541828506034 2.3997452882203145 >5k 1.347694552337356E-4 0.707077095651391 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8157 0.7067930812781716 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3629 0.3144479700819523 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3223 0.27926861603034786 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2786 0.2414031536644583 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2264 0.19617255559810967 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1783 0.1544945523990413 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1609 0.13941768637692512 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1599 0.13855119982392994 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1595 0.13820460520273184 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1495 0.12953973967278 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1398 0.12113482010872674 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1380 0.11957514431333541 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1309 0.1134230897870696 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1233 0.1068377919843062 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1196 0.10363179173822402 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1185 0.1026786565299293 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.664865529951841E-5 2 0.0 0.0 0.0 0.0 8.664865529951841E-5 3 0.0 0.0 0.0 0.0 8.664865529951841E-5 4 0.0 0.0 0.0 0.0 8.664865529951841E-5 5 0.0 0.0 0.0 0.0 8.664865529951841E-5 6 0.0 0.0 0.0 0.0 1.7329731059903682E-4 7 0.0 0.0 0.0 8.664865529951841E-5 1.7329731059903682E-4 8 0.0 0.0 0.0 8.664865529951841E-5 1.7329731059903682E-4 9 0.0 0.0 0.0 2.5994596589855524E-4 1.7329731059903682E-4 10-11 0.0 0.0 0.0 2.5994596589855524E-4 1.7329731059903682E-4 12-13 8.664865529951841E-5 0.0 0.0 3.0327029354831447E-4 1.7329731059903682E-4 14-15 8.664865529951841E-5 0.0 0.0 3.4659462119807365E-4 1.7329731059903682E-4 16-17 8.664865529951841E-5 0.0 0.0 4.3324327649759206E-4 1.7329731059903682E-4 18-19 8.664865529951841E-5 0.0 0.0 5.198919317971105E-4 1.7329731059903682E-4 20-21 8.664865529951841E-5 0.0 0.0 5.632162594468697E-4 1.7329731059903682E-4 22-23 8.664865529951841E-5 0.0 0.0 7.798378976956657E-4 1.7329731059903682E-4 24-25 1.7329731059903682E-4 0.0 0.0 9.964595359444617E-4 1.7329731059903682E-4 26-27 1.7329731059903682E-4 0.0 0.0 0.0013430541571425353 1.7329731059903682E-4 28-29 2.5994596589855524E-4 0.0 0.0 0.0024694866760362746 1.7329731059903682E-4 30-31 2.5994596589855524E-4 0.0 0.0 0.004635703058524235 1.7329731059903682E-4 32-33 2.5994596589855524E-4 0.0 0.0 0.006975216751611232 1.7329731059903682E-4 34-35 2.5994596589855524E-4 0.0 0.0 0.009964595359444616 1.7329731059903682E-4 36-37 3.4659462119807365E-4 0.0 0.0 0.013213919933176558 2.5994596589855524E-4 38-39 6.065405870966288E-4 0.0 0.0 0.021142271893082494 2.5994596589855524E-4 40-41 6.065405870966288E-4 0.0 0.0 0.033489705273263864 2.5994596589855524E-4 42-43 6.065405870966288E-4 0.0 0.0 0.05558511237464106 2.5994596589855524E-4 44-45 6.065405870966288E-4 0.0 0.0 0.0746478165405351 2.5994596589855524E-4 46-47 7.798378976956657E-4 0.0 0.0 0.11849203612209142 2.5994596589855524E-4 48-49 7.798378976956657E-4 0.0 0.0 0.15713733638567662 2.5994596589855524E-4 50-51 7.798378976956657E-4 0.0 0.0 0.18408506818382686 2.5994596589855524E-4 52-53 7.798378976956657E-4 0.0 0.0 0.21467204350455685 2.5994596589855524E-4 54-55 7.798378976956657E-4 0.0 0.0 0.25119445171330385 2.5994596589855524E-4 56-57 7.798378976956657E-4 0.0 0.0 0.330521295640013 2.5994596589855524E-4 58-59 7.798378976956657E-4 0.0 0.0 0.4236252757593455 2.5994596589855524E-4 60-61 7.798378976956657E-4 0.0 0.0 0.520325175073608 2.5994596589855524E-4 62-63 7.798378976956657E-4 0.0 0.0 0.6204043719445518 2.5994596589855524E-4 64-65 7.798378976956657E-4 0.0 0.0 0.7470413816647979 2.5994596589855524E-4 66-67 7.798378976956657E-4 0.0 0.0 0.9151830972735133 2.5994596589855524E-4 68-69 7.798378976956657E-4 0.0 0.0 1.1017376521333766 2.5994596589855524E-4 70-71 7.798378976956657E-4 0.0 0.0 1.232620445963299 2.5994596589855524E-4 72-73 8.231622253454249E-4 0.0 0.0 1.3545784282973714 3.4659462119807365E-4 74-75 8.664865529951841E-4 0.0 0.0 1.5001914935282121 3.4659462119807365E-4 76-77 8.664865529951841E-4 0.0 0.0 1.6812871831042053 3.4659462119807365E-4 78-79 8.664865529951841E-4 0.0 0.0 1.8629894132672955 3.4659462119807365E-4 80-81 8.664865529951841E-4 0.0 0.0 2.1302138662110104 3.4659462119807365E-4 82-83 8.664865529951841E-4 0.0 0.0 2.4430588361699215 3.4659462119807365E-4 84-85 9.098108806449433E-4 0.0 0.0 2.837570163748629 3.4659462119807365E-4 86-87 9.964595359444617E-4 0.0 0.0 3.2725464133522113 3.4659462119807365E-4 88-89 0.001039783863594221 0.0 0.0 3.786242966295406 3.4659462119807365E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGGT 50 0.0016167674 37.99546 6 GTAAGGT 625 0.0 37.233936 4 GGTAAGG 670 0.0 32.60804 3 TAAGGTG 780 0.0 32.271786 5 AGGTAAG 650 0.0 32.148613 2 TATACCG 60 0.0039532706 31.662884 5 GGTGTGC 1225 0.0 23.650234 8 AAGGTAA 875 0.0 23.040873 1 AAGGTGT 1080 0.0 22.427876 6 CTCTACG 130 2.7396932E-4 22.154684 1 GAGTACT 4490 0.0 22.105688 12-13 AGGTGTG 1260 0.0 21.10859 7 GTACTTT 4755 0.0 20.773846 14-15 GTACATG 3985 0.0 20.718475 1 GGACCAT 585 0.0 20.296719 6 TACATGG 3965 0.0 20.00305 2 ACATGGG 3850 0.0 19.984625 3 GTGTGCA 1405 0.0 19.944237 9 AGTACTT 4835 0.0 19.104126 12-13 AGAGTAC 6810 0.0 19.00388 10-11 >>END_MODULE