FastQCFastQC Report
Thu 26 May 2016
SRR936641_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936641_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242943
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48820.3927774644533176No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43860.35287217515203834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40030.32205821184076827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32670.262843911587257No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30520.24554625594254928No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC29410.23661583837714198No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT19540.1572075308360882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19000.15286300337183603No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC15010.12076177266375047No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14460.11633679098719732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG42500.024.8227941
TACATGG42400.024.3229062
ACATGGG41950.024.0173743
GAGTACT42200.022.74982312-13
GTGTAGC6700.021.278111
GTACTTT45300.021.24459314-15
AGAGTAC64250.020.04594410-11
CATGGGG26150.019.9929814
TACCTGG9150.019.7371332
AGTACTT45350.019.38802112-13
GTATAGC4450.019.2220231
AGCACCG4250.019.0084465
GTATCAA99300.018.9510171
CATGGGT8100.018.7767984
ATCACGC1350.00937244517.601833
CCCTATA3007.63539E-817.4258122
GTGTTAC5200.017.3634831
ACTTTTT56700.017.30637616-17
GTGTAAG5950.016.7721581
TATCAAC113550.016.699652
Produced by FastQC (version 0.11.5)