##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936641_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1242943 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.3742102413385 31.0 31.0 34.0 30.0 34.0 2 31.430051096470233 31.0 31.0 34.0 28.0 34.0 3 31.463150763952974 33.0 31.0 34.0 28.0 34.0 4 35.08698870342405 37.0 35.0 37.0 32.0 37.0 5 35.053971099237856 37.0 35.0 37.0 32.0 37.0 6 35.16948806180171 37.0 35.0 37.0 32.0 37.0 7 35.02220214442657 37.0 35.0 37.0 32.0 37.0 8 34.97434073807085 37.0 35.0 37.0 32.0 37.0 9 36.45334580909985 39.0 37.0 39.0 32.0 39.0 10-11 36.36381193666966 39.0 37.0 39.0 32.0 39.0 12-13 36.26537138066669 39.0 35.0 39.0 32.0 39.0 14-15 37.335232589105054 40.0 37.0 41.0 32.0 41.0 16-17 37.20750428619816 39.5 37.0 41.0 31.0 41.0 18-19 37.17444042083989 39.5 36.5 41.0 31.0 41.0 20-21 37.07418682916272 39.0 36.0 41.0 31.0 41.0 22-23 36.94419776289017 39.0 36.0 41.0 31.0 41.0 24-25 37.046928539764096 39.0 36.0 41.0 31.0 41.0 26-27 37.01478426605243 39.0 36.0 41.0 31.0 41.0 28-29 36.921463413849224 39.0 36.0 41.0 30.0 41.0 30-31 36.73567331728003 39.0 36.0 41.0 30.0 41.0 32-33 36.54384553434872 39.0 35.5 41.0 30.0 41.0 34-35 36.315576015955685 39.0 35.0 40.0 30.0 41.0 36-37 36.11366812476517 39.0 35.0 40.0 29.5 41.0 38-39 35.89235789573617 38.0 35.0 40.0 28.5 41.0 40-41 35.71568768640235 38.0 35.0 40.0 27.5 41.0 42-43 35.47584523184088 38.0 35.0 40.0 27.0 41.0 44-45 35.19948018533432 38.0 34.0 40.0 26.5 41.0 46-47 34.93289475060401 38.0 34.0 40.0 25.0 41.0 48-49 34.846822018387 38.0 34.0 40.0 24.0 41.0 50-51 33.915359755033016 37.5 33.0 39.5 23.5 40.5 52-53 34.06854980477785 37.5 33.5 39.5 24.0 40.5 54-55 34.49017050661213 38.0 34.0 40.0 24.0 41.0 56-57 34.36666685439316 38.0 34.0 40.0 23.5 41.0 58-59 34.0998123003227 38.0 33.5 40.0 23.0 41.0 60-61 33.7525200270648 37.0 33.0 40.0 22.0 41.0 62-63 33.431636848994685 37.0 33.0 40.0 21.5 41.0 64-65 33.08942485697253 36.0 32.0 39.5 20.5 41.0 66-67 32.6841295216273 36.0 32.0 39.0 19.5 41.0 68-69 32.259801535549094 35.0 31.5 39.0 18.0 40.0 70-71 31.80133682719159 35.0 31.0 38.0 15.0 40.0 72-73 31.42276838117275 35.0 31.0 37.5 12.5 40.0 74-75 31.0071853657006 35.0 30.5 37.0 9.5 39.0 76-77 30.569356358256172 34.5 30.0 36.5 7.0 39.0 78-79 30.11222075348588 34.0 30.0 36.0 6.0 38.5 80-81 29.63230654985788 34.0 29.0 35.5 2.0 37.0 82-83 29.173978613661284 34.0 29.0 35.0 2.0 37.0 84-85 28.833703959071332 34.0 29.0 35.0 2.0 36.5 86-87 28.53675832278713 34.0 29.0 35.0 2.0 36.0 88-89 28.108407223822816 34.0 27.5 35.0 2.0 36.0 90-91 27.682260570275545 33.5 26.5 35.0 2.0 35.0 92-93 27.32322922290081 33.0 26.0 35.0 2.0 35.0 94-95 26.86165697059318 33.0 25.0 35.0 2.0 35.0 96-97 26.28616879454649 33.0 24.5 35.0 2.0 35.0 98-99 25.575488980588812 33.0 22.0 35.0 2.0 35.0 100-101 23.215249210945313 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4440819496381785 1101 2 0.42040430817970176 1101 3 0.5624643209012135 1101 4 0.532047920584219 1101 5 0.3562458231169998 1101 6 0.3423526109252606 1101 7 0.40210347147441183 1101 8 0.42797623337012425 1101 9 0.6498324285866559 1101 10-11 0.4502741504983945 1101 12-13 0.5839214566986044 1101 14-15 0.7900567949658139 1101 16-17 0.8045921656466035 1101 18-19 1.0283264075013534 1101 20-21 0.8817582173750012 1101 22-23 0.6602849830033577 1101 24-25 0.674051052273704 1101 26-27 0.5120115888904806 1101 28-29 0.6641654315218162 1101 30-31 0.5931676031253517 1101 32-33 0.44025169722148405 1101 34-35 0.5618757941081043 1101 36-37 0.5584128849847261 1101 38-39 0.4017123181008344 1101 40-41 0.5623893818850902 1101 42-43 0.5107348156032643 1101 44-45 0.4696725909536923 1101 46-47 0.5324085566119479 1101 48-49 0.681853162088963 1101 50-51 0.600988962164692 1101 52-53 0.7533132748149498 1101 54-55 0.71331833444426 1101 56-57 0.46899457784949306 1101 58-59 0.5607349011218759 1101 60-61 0.2955413916255196 1101 62-63 0.3150138377654059 1101 64-65 0.5582029105935078 1101 66-67 0.54355599406896 1101 68-69 0.5434433601911266 1101 70-71 0.4388325989412323 1101 72-73 0.3972531590497361 1101 74-75 0.28898946942674897 1101 76-77 0.2815534877855761 1101 78-79 0.19518544159029005 1101 80-81 0.4604550445399269 1101 82-83 0.49665243249398117 1101 84-85 0.4218290117311838 1101 86-87 0.24682565876268114 1101 88-89 0.3205139157292045 1101 90-91 0.18874806149641898 1101 92-93 0.14430267773408545 1101 94-95 0.21417693013905748 1101 96-97 0.2536343023152483 1101 98-99 0.10289250919557347 1101 100-101 0.41580568978594457 1102 1 0.35137372706221726 1102 2 0.41596194233545347 1102 3 0.4183140822816753 1102 4 0.5435800424052601 1102 5 0.4646529561742909 1102 6 0.40412658832330806 1102 7 0.41394173309346627 1102 8 0.34687153099091006 1102 9 0.5886096055565204 1102 10-11 0.4786853106295439 1102 12-13 0.4555356198762581 1102 14-15 0.5068419110241251 1102 16-17 0.502854539781282 1102 18-19 0.6759636092397798 1102 20-21 0.6259998613251625 1102 22-23 0.4132246441846732 1102 24-25 0.32511563287502554 1102 26-27 0.23761260654848826 1102 28-29 0.4175261633397511 1102 30-31 0.258599951109737 1102 32-33 0.4187986683636993 1102 34-35 0.22199011883099473 1102 36-37 0.37856525630969884 1102 38-39 0.29352913966089034 1102 40-41 0.44717590330999 1102 42-43 0.5195866732195 1102 44-45 0.22701737781926568 1102 46-47 0.3521161006465192 1102 48-49 0.523166548051627 1102 50-51 0.604413387497658 1102 52-53 0.5534118199831539 1102 54-55 0.5625270195686909 1102 56-57 0.6731444928648855 1102 58-59 0.8334691198977993 1102 60-61 0.6608972817089978 1102 62-63 0.7360078110233772 1102 64-65 0.7408298109331923 1102 66-67 0.7434797929967587 1102 68-69 0.701082370003526 1102 70-71 0.7067743943689635 1102 72-73 0.848177048894879 1102 74-75 0.4475503658205149 1102 76-77 0.665001674908904 1102 78-79 0.6234584535692989 1102 80-81 0.8986335538343013 1102 82-83 0.8348105495904008 1102 84-85 0.785373232238129 1102 86-87 0.7413028146212248 1102 88-89 0.9489717929989929 1102 90-91 0.8954841130018707 1102 92-93 0.5487992601147162 1102 94-95 0.590508651101878 1102 96-97 0.4059836684363596 1102 98-99 0.30920569226357486 1102 100-101 0.24399808323927985 1103 1 0.23970908691793014 1103 2 0.3660766369876711 1103 3 0.31078943900484646 1103 4 0.40989102677965406 1103 5 0.3692302343692262 1103 6 0.25062778540183217 1103 7 0.3570144395049013 1103 8 0.4027947684191773 1103 9 0.516987971304296 1103 10-11 0.44402653819511784 1103 12-13 0.5024369382912113 1103 14-15 0.5628342569723159 1103 16-17 0.6386996562588791 1103 18-19 0.5647871188253291 1103 20-21 0.5617020307945495 1103 22-23 0.6216648620577843 1103 24-25 0.6853629110897828 1103 26-27 0.5601126129211949 1103 28-29 0.17590125209746788 1103 30-31 0.2321618353818522 1103 32-33 0.5907930637760259 1103 34-35 0.5810682168399097 1103 36-37 0.5410723831258153 1103 38-39 0.4681019342989714 1103 40-41 0.4215665233578676 1103 42-43 0.5293285670591885 1103 44-45 0.6678048297513186 1103 46-47 0.7354769216104202 1103 48-49 0.6330393232316993 1103 50-51 0.7245644972417082 1103 52-53 0.697753319167866 1103 54-55 0.8371710752871522 1103 56-57 0.8098807976946318 1103 58-59 0.5887513191574882 1103 60-61 0.513278367613438 1103 62-63 0.5818884475808161 1103 64-65 0.7116561024199015 1103 66-67 0.5593147924402047 1103 68-69 0.7448368432932284 1103 70-71 0.8129648750102234 1103 72-73 0.7655408194330668 1103 74-75 0.7918640233351155 1103 76-77 0.7585360913766728 1103 78-79 0.7770729566153101 1103 80-81 0.5640228458090206 1103 82-83 0.5812444508314911 1103 84-85 0.7293061131372447 1103 86-87 0.7385234625475867 1103 88-89 0.9790919200340475 1103 90-91 0.8925106056396928 1103 92-93 0.7962324025782443 1103 94-95 0.6879029483461174 1103 96-97 0.8900684577927436 1103 98-99 1.0957573725494356 1103 100-101 1.3292662427066197 1104 1 0.11894731725961805 1104 2 0.2051576660022434 1104 3 0.1731232751147509 1104 4 0.23979710350703698 1104 5 0.3544248049140535 1104 6 0.04702155451770551 1104 7 0.15896589508628267 1104 8 0.12351778284503467 1104 9 0.4251148039514945 1104 10-11 0.3927252132713832 1104 12-13 0.09583372473126417 1104 14-15 0.2144408375177207 1104 16-17 0.06582087371827328 1104 18-19 0.33047467852865964 1104 20-21 0.3978749216756441 1104 22-23 0.17339046703494176 1104 24-25 0.314137142250452 1104 26-27 -0.08298576487650422 1104 28-29 0.2816211056119968 1104 30-31 0.16403239689397964 1104 32-33 -0.06352837730704408 1104 34-35 -0.08435962297799904 1104 36-37 0.017750109104383682 1104 38-39 0.018769248880445843 1104 40-41 0.14178631804154662 1104 42-43 0.052925052631003666 1104 44-45 0.09609983883520101 1104 46-47 0.3338774871842034 1104 48-49 0.17879974676058197 1104 50-51 0.09275881584428447 1104 52-53 0.2449366513600424 1104 54-55 0.30175706902649324 1104 56-57 0.2718897014267654 1104 58-59 0.2741312899481443 1104 60-61 0.27544870716378966 1104 62-63 0.384319524092156 1104 64-65 0.08808424603096654 1104 66-67 0.376146794822688 1104 68-69 0.3005637764514191 1104 70-71 0.23862246754581662 1104 72-73 0.28409746289755944 1104 74-75 0.14245778274555576 1104 76-77 0.3460923379822667 1104 78-79 0.17114420375603245 1104 80-81 0.3618696037643119 1104 82-83 0.5228076428001849 1104 84-85 0.4618257084608217 1104 86-87 0.4856138287147367 1104 88-89 0.5312155472313123 1104 90-91 0.5021810997456413 1104 92-93 0.5905124161126629 1104 94-95 0.3971075819562806 1104 96-97 0.47255312022669216 1104 98-99 0.6636428458518644 1104 100-101 1.058248675219005 1105 1 0.3530956626687747 1105 2 0.32153232369805096 1105 3 0.3041182568453742 1105 4 0.35751423375493374 1105 5 0.2667633640402798 1105 6 0.31900640588555973 1105 7 0.37766265324744097 1105 8 0.44355061248079863 1105 9 0.42666509230688376 1105 10-11 0.44938812113181825 1105 12-13 0.41525450613622183 1105 14-15 0.3475957444697215 1105 16-17 0.404633412464527 1105 18-19 0.45591058752112446 1105 20-21 0.49480323749954636 1105 22-23 0.3605899780978419 1105 24-25 0.567509545539373 1105 26-27 0.6986241587226871 1105 28-29 0.4783873777209209 1105 30-31 0.4295298000733041 1105 32-33 0.3813462887189303 1105 34-35 0.1792211836103661 1105 36-37 0.2747923904133316 1105 38-39 0.195786224011826 1105 40-41 0.15892410693401615 1105 42-43 0.4861157793111275 1105 44-45 0.39664204517261936 1105 46-47 0.44650817219594785 1105 48-49 0.21123654927330904 1105 50-51 0.36994695403389954 1105 52-53 0.20435951969162858 1105 54-55 0.33207121286483243 1105 56-57 0.4016868924936645 1105 58-59 0.5112670971371642 1105 60-61 0.29610116986874147 1105 62-63 0.31110478898715854 1105 64-65 0.37068370606062473 1105 66-67 0.4443331766618641 1105 68-69 0.43371254538391923 1105 70-71 0.5696245956848642 1105 72-73 0.3955967793852473 1105 74-75 0.4664003115920572 1105 76-77 0.20826880955052118 1105 78-79 0.3372254202764253 1105 80-81 0.2967055388871991 1105 82-83 0.3734281965723021 1105 84-85 0.5093881255427561 1105 86-87 0.6157291007210723 1105 88-89 0.5906944907553964 1105 90-91 0.6507073612620999 1105 92-93 0.5798710632230133 1105 94-95 0.5355451315536683 1105 96-97 0.7915519127905668 1105 98-99 0.6806644508036719 1105 100-101 1.3831447892388553 1106 1 -0.06008570008440728 1106 2 0.0532363464161385 1106 3 0.01868573300079035 1106 4 0.05409323502258445 1106 5 0.0984900827046502 1106 6 0.11066370816788407 1106 7 0.24084373922079294 1106 8 0.14501367067652637 1106 9 0.18379466401776767 1106 10-11 0.27495012258214047 1106 12-13 0.43642235096238124 1106 14-15 0.5299078524802923 1106 16-17 0.584671774149335 1106 18-19 0.4471333454660922 1106 20-21 0.3053557454016058 1106 22-23 0.10702948232979992 1106 24-25 0.11743426142488289 1106 26-27 -0.10854582284377301 1106 28-29 0.11498320720240685 1106 30-31 0.06296812522318618 1106 32-33 -0.17062521402949216 1106 34-35 -0.0348276071385385 1106 36-37 -0.07769558350293693 1106 38-39 0.07074872034448987 1106 40-41 -0.22968972430778223 1106 42-43 -0.3977820003610546 1106 44-45 -0.010399787331294874 1106 46-47 0.07132729657526937 1106 48-49 -0.2928498281768839 1106 50-51 -0.37182161374118294 1106 52-53 -0.19267699863949872 1106 54-55 -0.06881808073767104 1106 56-57 -0.23804042401513925 1106 58-59 -0.12839047085380173 1106 60-61 -0.07802379069013199 1106 62-63 -0.0630317963246867 1106 64-65 -0.05207008720698525 1106 66-67 -0.16314679831723566 1106 68-69 -0.11269623628660952 1106 70-71 -0.07526936996122302 1106 72-73 0.018143541337980906 1106 74-75 -0.23070374101825308 1106 76-77 -0.07355561239479869 1106 78-79 0.028842866262841937 1106 80-81 -0.013395541107708908 1106 82-83 0.29230098302559426 1106 84-85 0.1616701752634846 1106 86-87 0.11245092038240401 1106 88-89 0.19489877641432685 1106 90-91 0.37818052745731734 1106 92-93 0.4126525898298574 1106 94-95 0.3568964814089135 1106 96-97 0.504952485171259 1106 98-99 0.8651991008709281 1106 100-101 1.2133032733710643 1107 1 0.1820368447644185 1107 2 0.1635782400731891 1107 3 0.06245962541154171 1107 4 0.0905067669650137 1107 5 0.15624770131287136 1107 6 0.10019838287158933 1107 7 0.26319445284740084 1107 8 0.2432940162851125 1107 9 0.31948438324074857 1107 10-11 0.23397681018025906 1107 12-13 0.28710781598808666 1107 14-15 0.12986351295939613 1107 16-17 0.24649604584274698 1107 18-19 0.29053155031467526 1107 20-21 0.3542610385817042 1107 22-23 0.3590798855797672 1107 24-25 0.11491409892652626 1107 26-27 0.25386804464097423 1107 28-29 0.26420127375961755 1107 30-31 0.37523818885638605 1107 32-33 0.046492237766251776 1107 34-35 0.30973660727300256 1107 36-37 0.2418911002604034 1107 38-39 0.1662159031072008 1107 40-41 0.16037642546393016 1107 42-43 0.3375350272133346 1107 44-45 0.41541659911758444 1107 46-47 0.4931417416721544 1107 48-49 0.41011852299212137 1107 50-51 0.3343661461543235 1107 52-53 0.47401263669789984 1107 54-55 0.4109984495150627 1107 56-57 0.4080858597338235 1107 58-59 0.46380277755537236 1107 60-61 0.31787001016549965 1107 62-63 0.38940448059359056 1107 64-65 0.5887486360544898 1107 66-67 0.17203241723274942 1107 68-69 0.18994615948555804 1107 70-71 0.3413675372972733 1107 72-73 0.3207475894164027 1107 74-75 0.2040386703326611 1107 76-77 0.08622424787579419 1107 78-79 0.2356507802681378 1107 80-81 0.27977377594383057 1107 82-83 0.4417449414662791 1107 84-85 0.3696069495034209 1107 86-87 0.1692816033308624 1107 88-89 -0.07140139015846714 1107 90-91 -0.06853894717927744 1107 92-93 -0.14502713502543685 1107 94-95 0.148624764031414 1107 96-97 0.1396481602704256 1107 98-99 0.41718119729140923 1107 100-101 0.6555073070691044 1108 1 -0.11584762297382056 1108 2 -0.07801862075876542 1108 3 0.04306713189081535 1108 4 0.09441767919988564 1108 5 0.15534328139832354 1108 6 -0.04981995274854256 1108 7 -0.19426141461971014 1108 8 0.021312148633953143 1108 9 0.07983027407032495 1108 10-11 -0.24836571293841558 1108 12-13 -0.09697810184361799 1108 14-15 -0.0638423562245336 1108 16-17 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-2.7932781636492514 2316 72-73 -2.261136491478112 2316 74-75 -1.729419619009036 2316 76-77 -1.8268888175402616 2316 78-79 -2.728356485106527 2316 80-81 -2.1603722301431603 2316 82-83 -2.2094458972020696 2316 84-85 -2.252606748996712 2316 86-87 -2.8960299892387376 2316 88-89 -3.1241422501141543 2316 90-91 -2.716775920713122 2316 92-93 -3.6021713761747165 2316 94-95 -3.933632998596984 2316 96-97 -4.925190892963734 2316 98-99 -6.214908705129545 2316 100-101 -6.58232167455261 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3452.0 3 1299.0 4 1251.0 5 1367.0 6 1448.0 7 1743.0 8 2171.0 9 2735.0 10 3449.0 11 4258.0 12 5182.0 13 6333.0 14 7077.0 15 8442.0 16 10222.0 17 12121.0 18 10643.0 19 8552.0 20 8578.0 21 9049.0 22 10208.0 23 11445.0 24 13058.0 25 14838.0 26 17090.0 27 20346.0 28 24165.0 29 28787.0 30 35590.0 31 43002.0 32 53887.0 33 68625.0 34 89910.0 35 122172.0 36 169950.0 37 216233.0 38 168109.0 39 26149.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.680578794749024 17.86588953194051 14.543751252501986 22.909780420808474 2 15.575476898440046 19.343588175413327 40.32092317720383 24.760011748942794 3 20.189284602057327 23.181851467675884 29.741308897953534 26.887555032313255 4 12.813472495238647 15.728724440430364 37.50682205661462 33.95098100771637 5 13.720377792571496 36.582817409111875 34.80260716479281 14.894197633523817 6 32.49334532127992 35.06635360632474 17.398111173984333 15.042189898411007 7 28.680545046509902 32.00828130684947 20.759452506447634 18.551721140192996 8 26.329506590888418 33.40518368927163 19.947086156840367 20.318223562999588 9 25.593803675747374 17.088265264235595 20.977226592746057 36.340704467270974 10-11 25.2702118025859 25.50815515419832 27.42091864969563 21.80071439352015 12-13 26.42165489592258 23.01019827086971 27.832775884737526 22.73537094847018 14-15 23.035300584754843 24.7153188384743 26.602623046608553 25.6467575301623 16-17 22.1498890453067 27.896472872241528 27.23158985156942 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22.213595576220172 25.95382321776399 28.79793453957981 23.034646666436025 44-45 22.352118160650985 25.68644914628318 28.96703563710559 22.99439705596025 46-47 23.03710414229557 26.090886563566528 28.57565244815895 22.29635684597895 48-49 22.808203197283838 26.08063205490253 28.425293459808675 22.685871288004954 50-51 22.257877005078694 26.421199778749937 28.921043898023836 22.399879318147534 52-53 22.74267471287996 26.611237316493195 27.99658219644566 22.64950577418118 54-55 22.269804782322193 26.21925560940051 28.3674305176169 23.143509090660398 56-57 22.63865363647702 26.313191256522806 28.051506531858657 22.99664857514152 58-59 23.0620005342654 26.050668314521396 28.032881460668733 22.854449690544467 60-61 22.531040270481427 26.14036936936212 28.962980187814658 22.365610172341793 62-63 22.24305208856734 26.39729626546869 28.756546824757308 22.603104821206664 64-65 22.053138483644837 26.82347754237731 28.346397777529265 22.776986196448583 66-67 22.24363311896511 26.41250904152221 28.008805376557778 23.335052462954902 68-69 22.178935255966653 26.221293271319823 28.45462445885704 23.14514701385648 70-71 22.721589313602355 26.15104304538517 28.010920053972683 23.116447587039797 72-73 22.509509955684575 26.016929652074005 28.5472456529365 22.92631473930492 74-75 22.135238990229272 26.997230661294267 28.042853142982192 22.824677205494265 76-77 22.355872805268923 26.39792345642159 27.929905389655662 23.316298348653824 78-79 22.367723915197512 26.450992847571076 28.114126680744363 23.06715655648705 80-81 22.817995946221046 26.394623070170287 27.697657932830218 23.08972305077845 82-83 22.5940493446024 26.270696528160798 28.226771633270214 22.90848249396659 84-85 22.397238118534634 26.60938093429548 27.9841258601711 23.009255086998785 86-87 22.497479678525252 26.63937558784034 27.874353224764402 22.98879150887 88-89 22.226254071687446 26.615369204546045 27.466737577154376 23.691639146612125 90-91 22.23005783648812 26.282916452282386 27.89925661788695 23.587769093342548 92-93 22.57629213523832 26.389601360542574 28.121339991204913 22.91276651301419 94-95 22.322705178741117 26.246649785851815 28.12892501072149 23.301720024685576 96-97 21.818025286267794 26.240889376299688 28.577859650633204 23.363225686799318 98-99 22.07504444034934 26.69148942162766 27.672033843066217 23.561432294956774 100-101 22.029334727274115 26.18508105097662 27.951892946635486 23.83369127511377 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 102.0 1 85.0 2 88.5 3 132.0 4 133.0 5 136.0 6 233.5 7 481.0 8 803.5 9 970.5 10 918.0 11 847.5 12 892.0 13 1007.5 14 1121.5 15 1232.5 16 1372.0 17 1480.5 18 1622.5 19 1817.5 20 1999.0 21 2332.5 22 2848.5 23 3367.0 24 4132.5 25 5256.0 26 6742.0 27 8484.5 28 10141.5 29 11954.0 30 14153.0 31 16999.0 32 20181.0 33 22131.5 34 23320.0 35 25006.0 36 27581.5 37 30138.0 38 31697.5 39 32946.0 40 34257.5 41 35815.5 42 37080.5 43 38202.0 44 39812.0 45 42232.0 46 44289.5 47 49223.5 48 58325.0 49 57527.0 50 48319.0 51 43790.0 52 42706.5 53 42382.5 54 41529.0 55 38976.0 56 35678.5 57 32367.5 58 29978.0 59 26919.0 60 22442.5 61 18743.0 62 15810.5 63 12401.0 64 8801.0 65 6414.5 66 4935.0 67 3941.5 68 3111.5 69 2490.0 70 1946.0 71 1404.0 72 1041.5 73 839.0 74 655.0 75 510.5 76 386.0 77 255.5 78 171.0 79 117.5 80 75.0 81 50.0 82 42.5 83 28.5 84 17.5 85 14.5 86 11.0 87 11.5 88 10.5 89 5.5 90 3.0 91 2.0 92 2.0 93 1.5 94 0.5 95 0.5 96 1.0 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03459531128941552 2 0.022366271019668642 3 0.021239912047455112 4 0.052214783783327154 5 0.054226139090851305 6 0.04698525998376434 7 0.05044479111270589 8 0.04392799991632762 9 0.04256027830721119 10-11 0.05072638085575927 12-13 0.042359142776458775 14-15 0.03672734791539113 16-17 0.04376709149172568 18-19 0.050122974263502026 20-21 0.010861318660630457 22-23 0.017699926706212593 24-25 0.005672021967218127 26-27 0.011384271040586737 28-29 0.005953611710271508 30-31 0.008327010973150015 32-33 0.0030572600674367203 34-35 0.008045421230096633 36-37 0.009614278369965477 38-39 0.010298139174523692 40-41 0.001930901095223192 42-43 0.001930901095223192 44-45 0.0020113553075241583 46-47 7.24087910708697E-4 48-49 0.0010459047599125624 50-51 4.424981676553148E-4 52-53 0.0031779413858881705 54-55 0.005953611710271508 56-57 0.01512539191258167 58-59 0.009252234414611128 60-61 0.009815413900717893 62-63 0.0055915677549171604 64-65 1.6090842460193269E-4 66-67 0.005309978011863778 68-69 0.016332205097096167 70-71 0.007401787531688903 72-73 0.0028158974305338216 74-75 0.0037813479781454175 76-77 0.008729282034654848 78-79 0.006315655665625856 80-81 0.012229040269746881 82-83 0.005752476179519093 84-85 0.01343585345426138 86-87 0.0037008937658444515 88-89 0.00494793405650943 90-91 0.003419304022791069 92-93 0.016372432203246648 94-95 0.00901087177770823 96-97 0.007602923062441319 98-99 0.021400820472057044 100-101 0.03399190469715828 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1242943.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.97046790624599 #Duplication Level Percentage of deduplicated Percentage of total 1 83.83600730463743 55.307006292783875 2 9.884758310970392 13.042042618297412 3 2.687017449387697 5.31791395225062 4 1.108197603693711 2.9243325779301865 5 0.6520989709616376 2.150963711776037 6 0.4022921638354482 1.5923641369944415 7 0.28125088802541415 1.2987976877458638 8 0.19886037674027862 1.0495129681254827 9 0.14925200001078104 0.8861601848988833 >10 0.7392867992506865 8.616408137916675 >50 0.03840473721368259 1.6844975716677364 >100 0.018668823385609025 2.434359288190105 >500 0.0021963216865671467 0.9762525862781002 >1k 0.0017082502006633363 2.7193882851445466 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4882 0.3927774644533176 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4386 0.35287217515203834 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4003 0.32205821184076827 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3267 0.262843911587257 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3052 0.24554625594254928 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2941 0.23661583837714198 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1954 0.1572075308360882 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1900 0.15286300337183603 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1501 0.12076177266375047 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1446 0.11633679098719732 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 8.045421230096633E-5 0.0 0.0 0.0 0.0 8 8.045421230096633E-5 0.0 0.0 0.0 0.0 9 8.045421230096633E-5 0.0 0.0 0.0 0.0 10-11 8.045421230096633E-5 0.0 0.0 0.0 8.045421230096633E-5 12-13 8.045421230096633E-5 0.0 0.0 0.0 8.045421230096633E-5 14-15 1.6090842460193266E-4 0.0 0.0 1.2068131845144949E-4 8.045421230096633E-5 16-17 3.620439553543485E-4 0.0 0.0 2.0113553075241583E-4 8.045421230096633E-5 18-19 4.0227106150483166E-4 0.0 0.0 2.815897430533822E-4 8.045421230096633E-5 20-21 4.0227106150483166E-4 0.0 0.0 4.0227106150483166E-4 8.045421230096633E-5 22-23 4.0227106150483166E-4 0.0 0.0 5.229523799562812E-4 1.2068131845144949E-4 24-25 6.436336984077306E-4 0.0 0.0 5.631794861067643E-4 1.6090842460193266E-4 26-27 6.436336984077306E-4 0.0 0.0 0.0012470402906649782 1.6090842460193266E-4 28-29 8.045421230096633E-4 0.0 0.0 0.0025745347936309225 1.6090842460193266E-4 30-31 8.849963353106297E-4 0.0 0.0 0.004585890101155081 1.6090842460193266E-4 32-33 8.849963353106297E-4 0.0 0.0 0.007562695956290836 1.6090842460193266E-4 34-35 0.0010056776537620792 0.0 0.0 0.010459047599125623 1.6090842460193266E-4 36-37 0.0010459047599125624 4.0227106150483165E-5 0.0 0.014521985320324424 2.0113553075241583E-4 38-39 0.0010459047599125624 8.045421230096633E-5 0.0 0.022768542081173473 2.41362636902899E-4 40-41 0.0010459047599125624 8.045421230096633E-5 0.0 0.03620439553543485 2.41362636902899E-4 42-43 0.0010459047599125624 8.045421230096633E-5 0.0 0.05587545044302112 2.41362636902899E-4 44-45 0.0010459047599125624 8.045421230096633E-5 0.0 0.07530514271370449 2.41362636902899E-4 46-47 0.001206813184514495 8.045421230096633E-5 0.0 0.11657815362410023 2.41362636902899E-4 48-49 0.001206813184514495 8.045421230096633E-5 0.0 0.15821320848985032 2.41362636902899E-4 50-51 0.001206813184514495 8.045421230096633E-5 0.0 0.18492400697377112 2.41362636902899E-4 52-53 0.001206813184514495 8.045421230096633E-5 0.0 0.21807114244176923 2.41362636902899E-4 54-55 0.001206813184514495 1.6090842460193266E-4 0.0 0.2523446368819809 2.815897430533822E-4 56-57 0.001206813184514495 1.6090842460193266E-4 0.0 0.32587978692506414 3.218168492038653E-4 58-59 0.001206813184514495 1.6090842460193266E-4 0.0 0.419448035831088 3.218168492038653E-4 60-61 0.001206813184514495 1.6090842460193266E-4 0.0 0.5123726510387041 3.218168492038653E-4 62-63 0.001206813184514495 1.6090842460193266E-4 0.0 0.6057397644139755 4.0227106150483166E-4 64-65 0.001206813184514495 1.6090842460193266E-4 0.0 0.725576313636265 4.0227106150483166E-4 66-67 0.0012872673968154613 1.6090842460193266E-4 0.0 0.8903465404286439 4.0227106150483166E-4 68-69 0.0012872673968154613 1.6090842460193266E-4 0.0 1.0710467012566143 4.0227106150483166E-4 70-71 0.0013274945029659445 1.6090842460193266E-4 0.0 1.200497528848869 4.0227106150483166E-4 72-73 0.0014079487152669108 1.6090842460193266E-4 0.0 1.3209374846634159 4.0227106150483166E-4 74-75 0.0014884029275678773 1.6090842460193266E-4 0.0 1.4652723415313493 4.0227106150483166E-4 76-77 0.0015286300337183604 1.6090842460193266E-4 0.0 1.6380879895538252 4.0227106150483166E-4 78-79 0.0015286300337183604 1.6090842460193266E-4 0.0 1.8201156448847615 4.0227106150483166E-4 80-81 0.0016090842460193266 1.6090842460193266E-4 0.0 2.063288501564432 4.0227106150483166E-4 82-83 0.0016090842460193266 1.6090842460193266E-4 0.0 2.3569463764629592 4.82725273805798E-4 84-85 0.00164931135216981 1.6090842460193266E-4 0.0 2.748476800625612 4.82725273805798E-4 86-87 0.0016895384583202932 1.6090842460193266E-4 0.0 3.161930997640278 4.82725273805798E-4 88-89 0.0016895384583202932 1.6090842460193266E-4 0.0 3.6354442641376155 4.82725273805798E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4250 0.0 24.822794 1 TACATGG 4240 0.0 24.322906 2 ACATGGG 4195 0.0 24.017374 3 GAGTACT 4220 0.0 22.749823 12-13 GTGTAGC 670 0.0 21.27811 1 GTACTTT 4530 0.0 21.244593 14-15 AGAGTAC 6425 0.0 20.045944 10-11 CATGGGG 2615 0.0 19.992981 4 TACCTGG 915 0.0 19.737133 2 AGTACTT 4535 0.0 19.388021 12-13 GTATAGC 445 0.0 19.222023 1 AGCACCG 425 0.0 19.008446 5 GTATCAA 9930 0.0 18.951017 1 CATGGGT 810 0.0 18.776798 4 ATCACGC 135 0.009372445 17.60183 3 CCCTATA 300 7.63539E-8 17.425812 2 GTGTTAC 520 0.0 17.363483 1 ACTTTTT 5670 0.0 17.306376 16-17 GTGTAAG 595 0.0 16.772158 1 TATCAAC 11355 0.0 16.69965 2 >>END_MODULE