FastQCFastQC Report
Thu 26 May 2016
SRR936641_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936641_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242943
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86320.6944807605819414No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38030.30596736938057495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34770.27973929617045995No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29740.23927082738307387No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25130.2021814355123284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20270.16308068833405875No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC18100.14562212426474905No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT17200.1383812451576621No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT16800.13516307666562344No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG16610.13363444663190507No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA15970.12848537704464325No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15810.12719810964782777No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA13760.11070499612612969No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13650.10981999979081905No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC13550.10901545766780939No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA13260.10668228551108136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG6750.028.1435835
GTAAGGT6900.022.7137074
GAGTACT47900.022.41169512-13
GTACATG43200.021.225311
GTACTTT50050.021.21126214-15
TACCTGG9650.021.162372
TACATGG42900.021.1445882
AGGTAAG8100.021.1076872
ACATGGG41700.020.7280543
GGTAAGG7500.020.263383
AGAGTAC73950.020.20193710-11
GTGTAGA6700.019.8547151
AGTACTT49900.019.79997312-13
TACACGA1456.1531406E-419.6519852
GGTGTGC12350.019.6129918
GTCTAGA4200.019.2300341
GTGTAGC5600.018.6644441
ACTTTTT58050.018.40934816-17
AAGGTAA9000.017.9480321
GTGTAAG4303.6379788E-1217.6779541
Produced by FastQC (version 0.11.5)