##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936641_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1242943 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.859534990743743 31.0 31.0 34.0 30.0 34.0 2 32.003105532594816 31.0 31.0 34.0 30.0 34.0 3 32.11297621853939 33.0 31.0 34.0 30.0 34.0 4 35.84343610286232 37.0 35.0 37.0 35.0 37.0 5 35.60300432119574 37.0 35.0 37.0 33.0 37.0 6 35.71846255218461 37.0 35.0 37.0 33.0 37.0 7 35.660879058814444 37.0 35.0 37.0 33.0 37.0 8 35.634026660916874 37.0 35.0 37.0 33.0 37.0 9 37.26892383641084 39.0 37.0 39.0 34.0 39.0 10-11 37.13657545036257 39.0 37.0 39.0 33.0 39.0 12-13 37.041884865194945 39.0 37.0 39.0 33.0 39.0 14-15 38.145392829759686 40.0 37.0 41.0 33.0 41.0 16-17 38.07797179758042 40.0 37.0 41.0 33.0 41.0 18-19 38.21864075826486 40.0 37.5 41.0 33.0 41.0 20-21 38.235050601676825 40.0 38.0 41.0 33.0 41.0 22-23 38.139871257169474 40.0 37.0 41.0 33.0 41.0 24-25 38.00401506746488 40.0 37.0 41.0 33.0 41.0 26-27 37.76728498410627 40.0 37.0 41.0 32.5 41.0 28-29 37.693929246956614 39.5 37.0 41.0 32.5 41.0 30-31 37.68589026206351 40.0 37.0 41.0 33.0 41.0 32-33 37.556658672199774 40.0 36.5 41.0 32.0 41.0 34-35 37.36709849124216 39.5 36.0 41.0 32.0 41.0 36-37 37.13427003490908 39.0 36.0 41.0 31.0 41.0 38-39 36.9483544297687 39.0 36.0 41.0 31.0 41.0 40-41 36.73012278117339 39.0 35.0 41.0 30.5 41.0 42-43 36.487096351160105 39.0 35.0 40.5 30.0 41.0 44-45 36.24634395945751 39.0 35.0 40.0 30.0 41.0 46-47 35.90941056830442 39.0 35.0 40.0 29.0 41.0 48-49 35.54822867983488 38.0 35.0 40.0 27.5 41.0 50-51 35.21688806324988 38.0 34.0 40.0 27.0 41.0 52-53 34.94540497834575 38.0 34.0 40.0 26.0 41.0 54-55 34.68515209466564 38.0 34.0 40.0 26.0 41.0 56-57 34.336050808444156 38.0 33.0 40.0 25.5 41.0 58-59 33.9737916380719 37.0 33.0 40.0 24.0 41.0 60-61 33.59059104077983 36.5 32.5 39.5 23.0 41.0 62-63 33.1931593001449 36.0 32.0 39.0 22.5 40.0 64-65 32.773868150027795 35.5 31.5 39.0 22.0 40.0 66-67 32.29169760801581 35.0 31.0 38.5 20.5 40.0 68-69 31.9919859559127 35.0 31.0 38.0 20.0 40.0 70-71 31.536683098098628 35.0 30.5 37.5 18.5 39.5 72-73 31.077852725346215 34.0 30.0 37.0 18.0 39.0 74-75 30.5510896316243 34.0 29.5 36.0 14.0 39.0 76-77 29.135213360548313 32.5 28.0 35.0 12.0 37.0 78-79 29.942551669706496 34.0 29.0 35.0 11.0 37.0 80-81 29.738627193684664 34.0 29.0 35.0 7.5 37.0 82-83 29.394495966428067 34.0 29.0 35.0 7.0 36.5 84-85 28.9701728076026 34.0 29.0 35.0 2.0 36.0 86-87 28.612387695976402 33.0 29.0 35.0 2.0 36.0 88-89 28.18683197861849 33.0 27.5 35.0 2.0 35.0 90-91 27.691751753700693 33.0 27.0 35.0 2.0 35.0 92-93 27.192920753405424 33.0 26.0 34.5 2.0 35.0 94-95 26.57631604989127 32.0 25.0 34.0 2.0 35.0 96-97 25.798074006611728 32.0 23.5 34.0 2.0 35.0 98-99 24.82272155682119 32.0 19.0 34.0 2.0 35.0 100-101 21.634806664505128 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2862058129660596 1101 2 0.36132385336842177 1101 3 0.28985542632178607 1101 4 0.1939668702888966 1101 5 0.20562597441020358 1101 6 0.23329243651417642 1101 7 0.1603836883959886 1101 8 0.20515386726775375 1101 9 0.346453023430243 1101 10-11 0.3394547081875743 1101 12-13 0.39976712944937276 1101 14-15 0.4691059432041058 1101 16-17 0.507727484086054 1101 18-19 0.3955424468813149 1101 20-21 0.3210754936181175 1101 22-23 0.28918976983482025 1101 24-25 0.28336358397381645 1101 26-27 0.3034515172315366 1101 28-29 0.1538854203230713 1101 30-31 0.24180364002512533 1101 32-33 0.44803703280719986 1101 34-35 0.21226086386965193 1101 36-37 0.15489830915214498 1101 38-39 0.3248165087947186 1101 40-41 0.4141058683034373 1101 42-43 0.39996536533182336 1101 44-45 0.3355148920003259 1101 46-47 0.3402151932379738 1101 48-49 0.17256600309298875 1101 50-51 0.23792276988306327 1101 52-53 0.1453545875601705 1101 54-55 0.24166134988524135 1101 56-57 0.27337323350416654 1101 58-59 0.2318187767197415 1101 60-61 0.17424247204577625 1101 62-63 0.2095954740686139 1101 64-65 0.1869739043059795 1101 66-67 0.13555868505822843 1101 68-69 0.008147738751990374 1101 70-71 0.0955959630107408 1101 72-73 0.09449310789133136 1101 74-75 0.15539438632663405 1101 76-77 -0.01411585892396161 1101 78-79 -0.09317215209126672 1101 80-81 1.4665400149738161E-4 1101 82-83 0.24923413954467222 1101 84-85 0.1289290288887237 1101 86-87 0.24326128199054153 1101 88-89 0.04406926833682334 1101 90-91 0.09844810146156036 1101 92-93 0.18859729023794003 1101 94-95 0.36928421262113176 1101 96-97 0.4761697640354363 1101 98-99 0.5419355839147784 1101 100-101 0.8834569347761345 1102 1 0.286501165959109 1102 2 0.3084859733967953 1102 3 0.2678362186177452 1102 4 0.23057934096548394 1102 5 0.16661754888821179 1102 6 0.22217560668424596 1102 7 0.2973607017146094 1102 8 0.25090969612327996 1102 9 0.27572265913929783 1102 10-11 0.3221840061258021 1102 12-13 0.34360152014642154 1102 14-15 0.435624984739988 1102 16-17 0.34644047956638246 1102 18-19 0.285762050820658 1102 20-21 0.18315977143895168 1102 22-23 0.25901316929127205 1102 24-25 0.3664286597408477 1102 26-27 -0.012645400670095341 1102 28-29 0.010288005457923077 1102 30-31 0.09854215708600123 1102 32-33 0.20787138857554766 1102 34-35 0.22330886361076097 1102 36-37 0.22695480838191173 1102 38-39 0.18734240354734766 1102 40-41 0.33553786289154175 1102 42-43 0.3575589678318991 1102 44-45 0.4307477405951943 1102 46-47 0.2991856543430629 1102 48-49 0.18539742125478398 1102 50-51 0.04698733302002722 1102 52-53 0.07750789579620943 1102 54-55 0.1497323455535664 1102 56-57 0.13082766053733508 1102 58-59 -0.020478941151885977 1102 60-61 0.059087421997567446 1102 62-63 0.0648445131440738 1102 64-65 0.3408731031545713 1102 66-67 0.1447576466484719 1102 68-69 0.030264412943740382 1102 70-71 0.20840835016663561 1102 72-73 0.16137746255531482 1102 74-75 0.016183605480094343 1102 76-77 -0.23586521672381977 1102 78-79 -0.17304381993164242 1102 80-81 0.10494560079840554 1102 82-83 0.1800335753434048 1102 84-85 0.2635944555864782 1102 86-87 0.19333070278534592 1102 88-89 0.022390487060871322 1102 90-91 0.3366673694689766 1102 92-93 0.35879085688472756 1102 94-95 0.5106978131384885 1102 96-97 0.5376331071333951 1102 98-99 0.4699583163357559 1102 100-101 1.010987532062071 1103 1 0.1581320902090937 1103 2 0.1727214363205043 1103 3 0.1903734982741483 1103 4 0.176477282976073 1103 5 0.0471807493377554 1103 6 0.13327939153695922 1103 7 0.10780311236706552 1103 8 0.13536789807253768 1103 9 0.23134507624376965 1103 10-11 0.07944967655350155 1103 12-13 0.13581599220689355 1103 14-15 0.1643612986331462 1103 16-17 0.3764181410870151 1103 18-19 0.31961602055282157 1103 20-21 0.26469323851426907 1103 22-23 0.13685238945260636 1103 24-25 0.06281029058295928 1103 26-27 0.22085118737996368 1103 28-29 0.3133253249083765 1103 30-31 0.2169785824874566 1103 32-33 0.20640212444816797 1103 34-35 0.20076730715660318 1103 36-37 0.33690136540394633 1103 38-39 0.3760063970738017 1103 40-41 0.5032030627015516 1103 42-43 0.2839792116433557 1103 44-45 0.26971695869743684 1103 46-47 0.32733094877509217 1103 48-49 0.2367845623684346 1103 50-51 0.35000241878681493 1103 52-53 0.36822336440720704 1103 54-55 0.24278880863050034 1103 56-57 0.3020209352587173 1103 58-59 0.3079828079904203 1103 60-61 0.27384087310428384 1103 62-63 0.5602165081625898 1103 64-65 0.43095690856423374 1103 66-67 0.5695580887164127 1103 68-69 0.3272674823250128 1103 70-71 0.4306631912326182 1103 72-73 0.3628469249448436 1103 74-75 0.5380524867915923 1103 76-77 0.30158423704979853 1103 78-79 0.252184301911214 1103 80-81 0.4793073028959327 1103 82-83 0.4881226143072972 1103 84-85 0.36631733127129706 1103 86-87 0.29877231897305023 1103 88-89 0.5379823887885529 1103 90-91 0.46847586862735113 1103 92-93 0.4539810455141655 1103 94-95 0.5861359937264154 1103 96-97 0.6891453233388347 1103 98-99 1.0047014917986061 1103 100-101 1.307306880266765 1104 1 0.13516678976962382 1104 2 0.21639286640187194 1104 3 0.09611319473359714 1104 4 0.009777600130810526 1104 5 0.0024187882299244734 1104 6 0.13497252321899822 1104 7 0.14193962518055514 1104 8 -0.08167669304646807 1104 9 0.006556750941783207 1104 10-11 0.011644306261551662 1104 12-13 0.030837340822507997 1104 14-15 0.10593728714086126 1104 16-17 0.14576168860671146 1104 18-19 0.08193460052920187 1104 20-21 -0.006344604009200339 1104 22-23 0.114381666936751 1104 24-25 0.0859467655053976 1104 26-27 0.01637562278669691 1104 28-29 0.09595733034873177 1104 30-31 -0.08281108894394862 1104 32-33 -0.09266944509886343 1104 34-35 0.09377743869173827 1104 36-37 0.022457662530939615 1104 38-39 -0.008345130832957182 1104 40-41 0.12568455425284242 1104 42-43 0.1315493316915095 1104 44-45 0.21127184122520504 1104 46-47 0.05995108833381835 1104 48-49 -0.002907576169228321 1104 50-51 -0.102982265916431 1104 52-53 0.028569814913190328 1104 54-55 0.25281531242325883 1104 56-57 0.009326196863177927 1104 58-59 -0.1433730601035208 1104 60-61 0.19385353004469863 1104 62-63 0.11029784097032547 1104 64-65 0.16514670151291 1104 66-67 -0.01842675885188072 1104 68-69 0.00511843488607866 1104 70-71 -0.13629843842823064 1104 72-73 -0.12309566147168027 1104 74-75 0.00702902292469787 1104 76-77 0.20008042766970746 1104 78-79 0.18915619539348683 1104 80-81 0.32429784010461304 1104 82-83 0.1874488183619043 1104 84-85 0.04234386298377757 1104 86-87 0.31388104302183706 1104 88-89 0.3764789317287516 1104 90-91 0.178612987697651 1104 92-93 0.06276891080549873 1104 94-95 0.30695789049922695 1104 96-97 0.6094431602916472 1104 98-99 0.6139598376348765 1104 100-101 1.1716160366723791 1105 1 0.15332743576987085 1105 2 0.1463400882027166 1105 3 0.21351772441661865 1105 4 0.07839408176541696 1105 5 0.055087379896427535 1105 6 0.09445984075540537 1105 7 -0.01893260492853699 1105 8 0.12885737603080116 1105 9 0.14640451052082426 1105 10-11 0.06610651491387642 1105 12-13 -0.0723245722403405 1105 14-15 0.06632733197471197 1105 16-17 0.20236781978671559 1105 18-19 0.16140945788600902 1105 20-21 0.16888160393112628 1105 22-23 0.21085949597186016 1105 24-25 0.1074421098176046 1105 26-27 0.31738957976619275 1105 28-29 0.1467279586977881 1105 30-31 0.35045285554029704 1105 32-33 0.440553681555663 1105 34-35 0.25740498191435535 1105 36-37 0.1746535828524287 1105 38-39 0.3401738089030957 1105 40-41 0.3337989283546108 1105 42-43 0.26114813319936303 1105 44-45 0.5036193530913167 1105 46-47 0.35815755903398383 1105 48-49 0.4564832867749331 1105 50-51 0.16897912944831717 1105 52-53 0.2937017867168734 1105 54-55 0.4777829343703601 1105 56-57 0.5313086368662283 1105 58-59 0.355519465805493 1105 60-61 0.18527608621415226 1105 62-63 0.2663262968287583 1105 64-65 0.24647524076072358 1105 66-67 0.561287481265687 1105 68-69 0.6429125885101854 1105 70-71 0.5571953924237363 1105 72-73 0.6560564237450741 1105 74-75 0.28823317959150785 1105 76-77 0.12075310199603351 1105 78-79 0.38501294454052015 1105 80-81 0.47578175016333546 1105 82-83 0.4790542875126569 1105 84-85 0.45446890671716744 1105 86-87 0.49732649751149083 1105 88-89 0.6118248485866467 1105 90-91 0.6691126487912911 1105 92-93 0.5547741403981163 1105 94-95 0.6762774822683184 1105 96-97 0.5427544552120871 1105 98-99 0.76677701321643 1105 100-101 0.866054464883419 1106 1 -0.09343008848988177 1106 2 0.11641963472663619 1106 3 0.02677215704564162 1106 4 -0.045961436394108546 1106 5 -0.17397339541923884 1106 6 -0.105205809206673 1106 7 -0.04974927150293951 1106 8 -0.0843212189240532 1106 9 -0.04201668195740638 1106 10-11 -0.08594885212385606 1106 12-13 -0.1418317380702021 1106 14-15 -0.15130077557086707 1106 16-17 -0.032013217473391364 1106 18-19 -0.09207104851036974 1106 20-21 -0.19196401381378791 1106 22-23 -0.1261908059949377 1106 24-25 -0.2761243643948177 1106 26-27 -0.19255207504507155 1106 28-29 -0.28270785329120685 1106 30-31 -0.20229612175822353 1106 32-33 -0.28608997548091253 1106 34-35 -0.24284024501743318 1106 36-37 -0.5917697020813364 1106 38-39 -0.3037885158260778 1106 40-41 -0.30996162387651083 1106 42-43 -0.03894043634711153 1106 44-45 -0.3001517721294178 1106 46-47 -0.5759184668504673 1106 48-49 -0.5315335303677031 1106 50-51 -0.3620954959301841 1106 52-53 -0.4358320053328981 1106 54-55 -0.5356281556439839 1106 56-57 -0.3089279545347736 1106 58-59 -0.5864732936340786 1106 60-61 -0.42263483496449084 1106 62-63 -0.32384884944938364 1106 64-65 -0.32909810866274114 1106 66-67 -0.4701699306214522 1106 68-69 -0.4382854806737022 1106 70-71 -0.27732458831238915 1106 72-73 -0.4666638791627733 1106 74-75 -0.39736375147370495 1106 76-77 -0.772462193964639 1106 78-79 -0.6212345298435054 1106 80-81 -0.5520357301437784 1106 82-83 -0.3949588539937956 1106 84-85 -0.6670795103789686 1106 86-87 -0.5748070321510035 1106 88-89 -0.5110645667989893 1106 90-91 -0.5144410935488288 1106 92-93 -0.4774671119752689 1106 94-95 -0.3411535580363605 1106 96-97 -0.2411051288431807 1106 98-99 -0.009475830696352716 1106 100-101 0.31979486754271846 1107 1 -0.07975673893866997 1107 2 -0.03981965488233641 1107 3 -0.031424063309792416 1107 4 -0.13748539771086854 1107 5 -0.1891770576239935 1107 6 -0.06739498317338644 1107 7 -0.1042149802761756 1107 8 -0.263854335752697 1107 9 -0.20029918462377339 1107 10-11 -0.1719741136671118 1107 12-13 -0.18748406829178776 1107 14-15 -0.0011168264774212844 1107 16-17 -0.07411059664819675 1107 18-19 -0.023734259890055398 1107 20-21 0.011272172028725436 1107 22-23 -0.1913231654566374 1107 24-25 -0.15540762203140446 1107 26-27 -0.02954321912454816 1107 28-29 -0.07024125772755241 1107 30-31 -0.2597799138657848 1107 32-33 -0.07490982520588574 1107 34-35 -0.3749492483369039 1107 36-37 -0.12672542247871377 1107 38-39 -0.27078013925172684 1107 40-41 -0.253939837593002 1107 42-43 -0.3751586347245208 1107 44-45 -0.15169107252368264 1107 46-47 0.2065506452805863 1107 48-49 0.00729650546342242 1107 50-51 0.25077315123508015 1107 52-53 0.09161236055203403 1107 54-55 0.22863958316029453 1107 56-57 -0.038050032021082814 1107 58-59 0.1784541453391526 1107 60-61 0.39646591161717737 1107 62-63 0.3673251891619529 1107 64-65 0.4283557243090783 1107 66-67 0.4758556904314375 1107 68-69 0.6347584873539063 1107 70-71 0.40416006661828874 1107 72-73 0.43270218552499173 1107 74-75 0.27515832840933285 1107 76-77 0.20020428895265852 1107 78-79 0.21052155485696034 1107 80-81 0.5959635987715828 1107 82-83 0.5456537504137877 1107 84-85 0.5057283796590184 1107 86-87 0.2843436862690716 1107 88-89 0.2462190982818413 1107 90-91 0.3195481451296125 1107 92-93 0.39921269676560556 1107 94-95 0.8067391163557716 1107 96-97 0.7686447722916725 1107 98-99 0.8605182973130425 1107 100-101 0.7901892496055858 1108 1 -0.004261368167707502 1108 2 0.1015912401843515 1108 3 0.048870158154279864 1108 4 -0.0729968928399174 1108 5 -0.0757839515424763 1108 6 0.09476881228624734 1108 7 0.02427582650484794 1108 8 -0.04427326690278477 1108 9 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-1.059949710978536 2316 92-93 -1.1282759774485065 2316 94-95 -1.1245891211977295 2316 96-97 -1.0049090398551606 2316 98-99 -1.2781499194408141 2316 100-101 -1.9234271987175902 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 24.0 5 45.0 6 91.0 7 220.0 8 549.0 9 1049.0 10 1719.0 11 2590.0 12 3620.0 13 4868.0 14 6044.0 15 8234.0 16 9839.0 17 11928.0 18 11101.0 19 8638.0 20 8035.0 21 8352.0 22 9014.0 23 10167.0 24 11612.0 25 13864.0 26 17007.0 27 21099.0 28 26006.0 29 32089.0 30 39130.0 31 47974.0 32 58958.0 33 75192.0 34 97928.0 35 132419.0 36 186187.0 37 233228.0 38 136665.0 39 7451.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52739687440141 19.241473335511714 14.653356018523736 22.577773771563137 2 16.05531677284496 20.881212798038103 38.498307959429646 24.56516246968729 3 20.725430705372823 23.71520545046556 29.435603582902285 26.123760261259328 4 13.535499328607436 16.79680177386018 36.48682678715529 33.180872110377095 5 13.788376024091441 37.612385562834945 33.89906599803207 14.700172415041543 6 32.63093954655896 36.19547202600286 16.38285033871305 14.790738088725119 7 29.097955417102796 32.80737732945115 19.84668645303928 18.247980800406776 8 26.78063274019806 34.20003974438088 18.96169011772865 20.057637397692414 9 25.917591364906094 17.526035297297994 19.826168531081446 36.73020480671447 10-11 25.59252706689548 26.213353994041544 26.52290257204899 21.671216367013987 12-13 26.806218863844084 23.55619150908178 27.014919609180904 22.62267001789323 14-15 22.984748359804026 25.615337486739847 25.81410401592063 25.585810137535496 16-17 22.27130977130977 28.491983290744898 26.557038998989462 22.679667938955863 18-19 22.212482792855344 27.538430965860865 28.76596111004286 21.483125131240936 20-21 23.09470345784159 25.891855056909286 28.825497227145576 22.18794425810355 22-23 22.380819245119827 26.00094536019229 29.004736857984774 22.613498536703105 24-25 23.059021827456093 26.108630413619434 28.83798765819475 21.994360100729725 26-27 22.596731557331708 26.014423693896177 29.305394583813232 22.08345016495888 28-29 22.02051329502093 27.25468227323838 28.832624949461017 21.892179482279676 30-31 22.47429755500426 26.288239891900282 28.57988786947579 22.657574683619668 32-33 22.438801276169336 26.461848273265332 28.710430966616613 22.388919483948726 34-35 23.033071632742143 26.31798733940414 28.67522373288436 21.973717294969354 36-37 23.279931121607376 25.89087280832334 28.90925110841105 21.91994496165823 38-39 22.682559944771274 26.55963419015369 28.792720493486044 21.965085371588987 40-41 22.6830529099997 26.5964015970553 28.55925095290296 22.16129454004204 42-43 22.48358325194016 26.838868873588222 27.790404670224905 22.887143204246712 44-45 22.841306978360773 26.421803998300785 28.030143338954467 22.70674568438397 46-47 23.589416409280233 27.04770049793112 27.462924687616407 21.89995840517224 48-49 23.395406989280186 27.0228781622808 27.21959045225322 22.36212439618579 50-51 22.683841661363342 27.298337558561176 27.910559797768343 22.107260982307142 52-53 23.257724327836144 27.578078014655155 26.97034976456414 22.193847892944568 54-55 22.644007385922496 27.32988442399057 27.23325596083369 22.792852229253242 56-57 23.17319443472272 27.25052517416634 27.066402450356865 22.509877940754077 58-59 23.570893544029538 26.909424286382066 26.813481403171707 22.706200766416693 60-61 22.835464180961605 27.3115516333459 27.83834656790787 22.01463761778463 62-63 22.51862716740333 27.31999303228682 27.773055306022204 22.38832449428764 64-65 22.375543019538647 28.020331198670117 27.220013533748677 22.384112248042555 66-67 22.69872507639404 27.258552190123403 27.025508126974216 23.017214606508336 68-69 22.668001187551944 27.159095754823625 27.169273622105845 23.003629435518587 70-71 23.24970785052406 27.246554044133177 26.847259627858893 22.656478477483873 72-73 22.9730175498162 26.983817931820177 27.408222096532437 22.634942421831187 74-75 22.48258954451408 28.13592365025974 26.746748123983444 22.63473868124273 76-77 22.80870924580963 27.28140193690769 26.87968306609736 23.030205751185324 78-79 22.896779501125394 27.624401851319195 26.68788053721244 22.790938110342967 80-81 23.25779080133497 27.45965808334862 26.55039601697997 22.73215509833644 82-83 22.915582153342605 27.440808704320702 27.012166127460723 22.63144301487597 84-85 22.583738948422013 27.887772248887753 26.855922865346248 22.672565937343986 86-87 22.61850835997158 27.880048628716853 26.46499641560335 23.036446595708217 88-89 22.254022597756318 28.065311015853027 26.019121797360445 23.66154458903021 90-91 22.57512684249001 27.603886926221545 26.327154049203337 23.493832182085107 92-93 22.82720196311121 27.712935213307322 26.62135687994046 22.838505943641007 94-95 22.40236413124372 27.64096164723242 26.74479788946737 23.21187633205649 96-97 22.067502061669046 27.620009252368405 27.24086328619989 23.071625399762656 98-99 22.14169798462785 27.92604696929834 26.163028794823706 23.769226251250103 100-101 21.57347152893772 27.909984584141938 26.358703523118958 24.157840363801377 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 176.0 1 142.5 2 118.5 3 145.5 4 143.0 5 136.0 6 232.5 7 454.5 8 697.0 9 860.5 10 885.5 11 838.0 12 884.0 13 1057.0 14 1214.0 15 1278.0 16 1382.0 17 1550.0 18 1704.5 19 1857.5 20 2042.0 21 2393.0 22 2806.5 23 3351.5 24 4092.5 25 5076.0 26 6448.5 27 8304.5 28 10145.5 29 12002.0 30 14343.0 31 16826.5 32 19708.5 33 21458.5 34 22904.0 35 25025.0 36 27701.5 37 30304.0 38 32010.0 39 33082.5 40 33839.0 41 35491.5 42 36839.0 43 38255.5 44 39711.5 45 41326.5 46 42885.0 47 48129.0 48 59353.5 49 58168.5 50 47482.0 51 43149.5 52 41993.5 53 42074.0 54 42091.0 55 40107.0 56 35979.0 57 32882.0 58 31338.5 59 27924.5 60 22738.5 61 18833.0 62 15949.5 63 12368.0 64 8648.5 65 6296.0 66 4931.0 67 4090.0 68 3349.0 69 2665.5 70 2061.5 71 1479.0 72 1190.5 73 954.0 74 734.0 75 582.0 76 426.0 77 294.0 78 199.5 79 139.0 80 101.5 81 67.5 82 49.0 83 35.5 84 18.0 85 8.5 86 6.0 87 5.0 88 2.5 89 1.5 90 2.5 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.034514857077114554 2 0.005229523799562812 3 0.0032181684920386537 4 3.2181684920386537E-4 5 9.65450547611596E-4 6 2.41362636902899E-4 7 0.0 8 0.0 9 0.001206813184514495 10-11 3.2181684920386537E-4 12-13 0.001206813184514495 14-15 0.002212490838276574 16-17 0.0031377142797376872 18-19 0.0 20-21 0.0 22-23 0.0024538534751794732 24-25 0.0010459047599125624 26-27 0.004626117207305564 28-29 0.0072811062132374545 30-31 0.0013274945029659445 32-33 3.620439553543485E-4 34-35 0.00889019045925678 36-37 0.013918578728067175 38-39 0.008970644671557747 40-41 0.0024538534751794732 42-43 4.022710615048317E-4 44-45 0.001206813184514495 46-47 0.0 48-49 8.849963353106296E-4 50-51 9.65450547611596E-4 52-53 0.002856124536684305 54-55 0.0030572600674367203 56-57 0.0017699926706212592 58-59 0.0012872673968154615 60-61 0.003982483508897834 62-63 0.005430659330315228 64-65 0.009976322325319824 66-67 0.003620439553543485 68-69 0.004022710615048317 70-71 4.022710615048317E-5 72-73 0.001930901095223192 74-75 0.00671792672713069 76-77 0.002735443218232855 78-79 0.003660666659693968 80-81 0.005390432224164745 82-83 0.005309978011863778 84-85 0.006235201453324892 86-87 0.004505435888854115 88-89 0.009694732582266444 90-91 0.012993355286606062 92-93 0.001448175821417394 94-95 0.002735443218232855 96-97 0.001448175821417394 98-99 0.0037008937658444515 100-101 0.005390432224164745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1242943.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.06525292249406 #Duplication Level Percentage of deduplicated Percentage of total 1 82.45430535873626 52.00001621997261 2 10.462060032765253 13.195849241133145 3 3.021630145213665 5.716796080383968 4 1.2833324364827792 3.2373473876170804 5 0.7060859024822327 2.2264743012524737 6 0.4553116838968238 1.7228607902111917 7 0.2993236517552201 1.321384526253924 8 0.2216586497971834 1.1183167049534326 9 0.16036108355862377 0.9101891064194833 >10 0.8620916452683732 9.644773883307876 >50 0.04594576931395946 1.9534775990128692 >100 0.023563141807078473 2.7437403939078857 >500 0.0019105142305135925 0.8285493709925585 >1k 0.002292617076616311 2.686861508453813 >5k 1.2736761536757282E-4 0.6933628861277524 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8632 0.6944807605819414 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3803 0.30596736938057495 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3477 0.27973929617045995 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2974 0.23927082738307387 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2513 0.2021814355123284 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2027 0.16308068833405875 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1810 0.14562212426474905 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1720 0.1383812451576621 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1680 0.13516307666562344 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1661 0.13363444663190507 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1597 0.12848537704464325 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1581 0.12719810964782777 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1376 0.11070499612612969 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1365 0.10981999979081905 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1355 0.10901545766780939 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1326 0.10668228551108136 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 8.045421230096633E-5 0.0 9 0.0 0.0 0.0 8.045421230096633E-5 8.045421230096633E-5 10-11 8.045421230096633E-5 0.0 0.0 8.045421230096633E-5 8.045421230096633E-5 12-13 8.045421230096633E-5 0.0 0.0 8.045421230096633E-5 8.045421230096633E-5 14-15 8.045421230096633E-5 0.0 0.0 2.0113553075241583E-4 8.045421230096633E-5 16-17 1.2068131845144949E-4 0.0 0.0 2.815897430533822E-4 8.045421230096633E-5 18-19 2.815897430533822E-4 0.0 0.0 4.0227106150483166E-4 8.045421230096633E-5 20-21 4.0227106150483166E-4 0.0 0.0 5.631794861067643E-4 8.045421230096633E-5 22-23 4.0227106150483166E-4 0.0 0.0 7.643150168591802E-4 1.2068131845144949E-4 24-25 4.82725273805798E-4 0.0 0.0 8.045421230096633E-4 1.6090842460193266E-4 26-27 5.229523799562812E-4 0.0 0.0 0.0014884029275678773 1.6090842460193266E-4 28-29 7.643150168591802E-4 0.0 0.0 0.0029768058551357546 1.6090842460193266E-4 30-31 8.045421230096633E-4 0.0 0.0 0.004988161162659913 1.6090842460193266E-4 32-33 8.045421230096633E-4 0.0 0.0 0.008045421230096633 1.6090842460193266E-4 34-35 8.045421230096633E-4 0.0 0.0 0.011223362615984804 1.6090842460193266E-4 36-37 8.849963353106297E-4 0.0 0.0 0.015406981655635053 1.6090842460193266E-4 38-39 8.849963353106297E-4 0.0 0.0 0.023532857098032652 1.6090842460193266E-4 40-41 8.849963353106297E-4 0.0 0.0 0.03716984608304645 1.6090842460193266E-4 42-43 9.654505476115961E-4 0.0 0.0 0.057202944945987065 1.6090842460193266E-4 44-45 0.0010459047599125624 0.0 0.0 0.07675331853512188 1.6090842460193266E-4 46-47 0.0011665860783640117 0.0 0.0 0.11899177999312921 1.6090842460193266E-4 48-49 0.001206813184514495 0.0 0.0 0.1606268348588793 1.6090842460193266E-4 50-51 0.001206813184514495 0.0 0.0 0.18753876887355253 1.6090842460193266E-4 52-53 0.001206813184514495 0.0 0.0 0.22052499591694874 2.815897430533822E-4 54-55 0.001206813184514495 0.0 0.0 0.2550398529940633 4.0227106150483166E-4 56-57 0.001206813184514495 8.045421230096633E-5 0.0 0.32889681988635033 4.0227106150483166E-4 58-59 0.001206813184514495 8.045421230096633E-5 0.0 0.421861662200117 4.0227106150483166E-4 60-61 0.0012470402906649782 8.045421230096633E-5 0.0 0.5143840063462283 4.0227106150483166E-4 62-63 0.0012872673968154613 8.045421230096633E-5 0.0 0.6085154347383589 4.0227106150483166E-4 64-65 0.0012872673968154613 8.045421230096633E-5 0.0 0.7285933465975512 4.0227106150483166E-4 66-67 0.0012872673968154613 8.045421230096633E-5 0.0 0.8938060715575855 4.0227106150483166E-4 68-69 0.0012872673968154613 8.045421230096633E-5 0.0 1.0752705474024151 4.0227106150483166E-4 70-71 0.0012872673968154613 8.045421230096633E-5 0.0 1.2039570599778107 4.0227106150483166E-4 72-73 0.0012872673968154613 8.045421230096633E-5 0.0 1.3243165615800563 4.0227106150483166E-4 74-75 0.0012872673968154613 8.045421230096633E-5 0.0 1.4706627737555142 4.0227106150483166E-4 76-77 0.0012872673968154613 8.045421230096633E-5 0.0 1.6456104584039655 4.0227106150483166E-4 78-79 0.0012872673968154613 8.045421230096633E-5 0.0 1.8284426558579114 4.0227106150483166E-4 80-81 0.0013677216091164278 8.045421230096633E-5 0.0 2.0735061865266546 4.0227106150483166E-4 82-83 0.001448175821417394 8.045421230096633E-5 0.0 2.3683708746096963 4.0227106150483166E-4 84-85 0.0014884029275678773 8.045421230096633E-5 0.0 2.7584531229509315 4.0227106150483166E-4 86-87 0.0015286300337183604 8.045421230096633E-5 0.0 3.169855737551923 4.0227106150483166E-4 88-89 0.0015286300337183604 8.045421230096633E-5 0.0 3.6423633263954986 4.0227106150483166E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 675 0.0 28.143583 5 GTAAGGT 690 0.0 22.713707 4 GAGTACT 4790 0.0 22.411695 12-13 GTACATG 4320 0.0 21.22531 1 GTACTTT 5005 0.0 21.211262 14-15 TACCTGG 965 0.0 21.16237 2 TACATGG 4290 0.0 21.144588 2 AGGTAAG 810 0.0 21.107687 2 ACATGGG 4170 0.0 20.728054 3 GGTAAGG 750 0.0 20.26338 3 AGAGTAC 7395 0.0 20.201937 10-11 GTGTAGA 670 0.0 19.854715 1 AGTACTT 4990 0.0 19.799973 12-13 TACACGA 145 6.1531406E-4 19.651985 2 GGTGTGC 1235 0.0 19.612991 8 GTCTAGA 420 0.0 19.230034 1 GTGTAGC 560 0.0 18.664444 1 ACTTTTT 5805 0.0 18.409348 16-17 AAGGTAA 900 0.0 17.948032 1 GTGTAAG 430 3.6379788E-12 17.677954 1 >>END_MODULE