FastQCFastQC Report
Thu 26 May 2016
SRR936640_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936640_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310489
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52810.40297934587776013No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT46650.35597399138794755No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40200.30675572248221844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32630.24899102548743254No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30450.23235601367123265No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30390.23189816930931892No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT20520.15658277177450555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18370.14017668213926252No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16020.12224444463097364No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC14990.11438478308478743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG43800.023.9727021
GAGTACT48200.023.61781512-13
TACATGG44050.023.2973582
TACCTGG10200.022.8241582
ACATGGG43350.022.7984983
GTACCTG11350.022.1859911
TAAGCGC550.002882582421.58542654-55
GTACTTT52150.021.3715814-15
CATGGGG26100.021.1186454
ACCGTAT1404.82688E-420.3729658
AGAGTAC70050.020.35764710-11
AGTACTT50950.020.01079612-13
GTGTAGC8350.019.9150051
GTACACG1707.940143E-519.5635621
GTATCAA115400.018.9799021
ATACCGT1300.007546467218.2764476
TCCGTAC1300.007549791618.2750513
GTACGGG1300.00755311718.2736571
ACTTTTT63050.018.0897616-17
GCGACAG3451.0768417E-917.905627
Produced by FastQC (version 0.11.5)