##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936640_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1310489 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.290309953002275 31.0 31.0 34.0 28.0 34.0 2 31.349370349541278 31.0 31.0 34.0 28.0 34.0 3 31.29585673744686 31.0 31.0 34.0 28.0 34.0 4 34.95431934186399 37.0 35.0 37.0 32.0 37.0 5 34.984614140217886 37.0 35.0 37.0 32.0 37.0 6 35.022125328789485 37.0 35.0 37.0 32.0 37.0 7 34.88261175790106 37.0 35.0 37.0 32.0 37.0 8 34.86322510146976 37.0 35.0 37.0 32.0 37.0 9 36.349028492417716 39.0 37.0 39.0 32.0 39.0 10-11 36.23398784728449 39.0 36.0 39.0 32.0 39.0 12-13 36.14755064712485 39.0 35.0 39.0 31.5 39.0 14-15 37.12924602953554 39.5 36.5 41.0 31.0 41.0 16-17 37.044933990289124 39.0 36.0 41.0 31.0 41.0 18-19 36.92402378043616 39.0 36.0 41.0 31.0 41.0 20-21 36.93007571982672 39.0 36.0 41.0 30.5 41.0 22-23 36.91204313809578 39.0 36.0 41.0 30.5 41.0 24-25 37.01260674450529 39.0 36.0 41.0 31.0 41.0 26-27 36.93440654595346 39.0 36.0 41.0 30.0 41.0 28-29 36.82667576759515 39.0 36.0 41.0 30.0 41.0 30-31 36.62837116526731 39.0 36.0 41.0 30.0 41.0 32-33 36.45505532667577 39.0 35.0 41.0 30.0 41.0 34-35 36.253048289607925 39.0 35.0 40.0 30.0 41.0 36-37 36.026540474586206 38.5 35.0 40.0 29.0 41.0 38-39 35.82571009752848 38.0 35.0 40.0 28.5 41.0 40-41 35.617648831848264 38.0 35.0 40.0 27.0 41.0 42-43 35.334404943498186 38.0 34.5 40.0 26.5 41.0 44-45 35.17609838770108 38.0 34.0 40.0 26.0 41.0 46-47 35.05230299529413 38.0 34.0 40.0 25.5 41.0 48-49 34.81622051005388 38.0 34.0 40.0 24.0 41.0 50-51 33.7981055163378 37.5 32.5 39.5 23.5 40.5 52-53 33.911604752119246 37.5 33.0 39.5 23.0 40.5 54-55 34.362468132124725 38.0 34.0 40.0 23.0 41.0 56-57 34.18320794756767 38.0 33.5 40.0 23.0 41.0 58-59 33.96194283202682 38.0 33.0 40.0 22.5 41.0 60-61 33.64755408095757 37.0 33.0 40.0 22.0 41.0 62-63 33.322129373081346 37.0 32.5 40.0 20.5 41.0 64-65 32.972682334609445 36.0 32.0 39.0 20.0 41.0 66-67 32.57437948735166 36.0 32.0 39.0 18.5 41.0 68-69 32.127333766250615 35.0 31.0 39.0 17.5 40.0 70-71 31.718688214857202 35.0 31.0 38.0 14.0 40.0 72-73 31.315370064151626 35.0 31.0 37.0 12.0 39.5 74-75 30.92097072161613 35.0 30.0 37.0 8.0 39.0 76-77 30.47466098532685 34.5 30.0 36.0 7.0 39.0 78-79 29.99543300248991 34.0 29.5 36.0 4.5 38.5 80-81 29.540511595290003 34.0 29.0 35.5 2.0 37.0 82-83 29.136971771605864 34.0 29.0 35.0 2.0 37.0 84-85 28.773422363713088 34.0 29.0 35.0 2.0 36.5 86-87 28.464188940158976 34.0 29.0 35.0 2.0 36.0 88-89 28.102317913389584 34.0 27.0 35.0 2.0 36.0 90-91 27.739490754977723 34.0 27.0 35.0 2.0 35.0 92-93 27.30913193472055 33.0 26.0 35.0 2.0 35.0 94-95 26.824778384251985 33.0 25.0 35.0 2.0 35.0 96-97 26.226720331113043 33.0 24.5 35.0 2.0 35.0 98-99 25.503872218690887 33.0 21.5 35.0 2.0 35.0 100-101 23.08964401837787 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5897331546452662 1101 2 0.360509207629228 1101 3 0.5189709944969287 1101 4 0.6828688783329326 1101 5 0.609263330306419 1101 6 0.5587408793181154 1101 7 0.6777486675543614 1101 8 0.5931198950455752 1101 9 0.8471372082426853 1101 10-11 0.8942313851693058 1101 12-13 0.9130047261932006 1101 14-15 1.1823735293324305 1101 16-17 1.0233645176660247 1101 18-19 1.2029003618407685 1101 20-21 1.124369708936392 1101 22-23 1.0800598470702383 1101 24-25 0.9693199961666679 1101 26-27 0.9512440671026567 1101 28-29 0.911705142562532 1101 30-31 0.833845580369811 1101 32-33 0.70796081698807 1101 34-35 0.9030110797220914 1101 36-37 0.9211207717996288 1101 38-39 0.8164973634019645 1101 40-41 0.8642837108368582 1101 42-43 0.9796447143877245 1101 44-45 0.7814311012525081 1101 46-47 1.0710164262074429 1101 48-49 1.2499358798893567 1101 50-51 1.123758739047645 1101 52-53 1.0651838431086489 1101 54-55 1.164653018125513 1101 56-57 1.202843041628931 1101 58-59 1.0499678770014853 1101 60-61 0.9260590539149831 1101 62-63 1.1056534884398275 1101 64-65 0.789185765726863 1101 66-67 0.9184594869395184 1101 68-69 0.992017265123998 1101 70-71 0.8751282920355052 1101 72-73 0.9375912580069432 1101 74-75 0.710870761449705 1101 76-77 0.6900592439071751 1101 78-79 0.8993252649737578 1101 80-81 0.8826452127958646 1101 82-83 0.6970417457899956 1101 84-85 0.7551216106396446 1101 86-87 0.6914602235464642 1101 88-89 0.8422358440836817 1101 90-91 0.568859171309267 1101 92-93 0.7431763878247253 1101 94-95 0.8128135128542588 1101 96-97 0.6363050915850188 1101 98-99 0.46888495404941466 1101 100-101 1.1348895159014276 1102 1 0.1315875601742853 1102 2 0.3548350314080686 1102 3 0.46546255809928994 1102 4 0.30343858445663585 1102 5 0.3536246258598581 1102 6 0.2900237152292817 1102 7 0.41363727637424574 1102 8 0.3104736025816095 1102 9 0.2747718456949926 1102 10-11 0.33218618399367017 1102 12-13 0.18901272941018732 1102 14-15 0.48147185970704953 1102 16-17 0.15305161411328783 1102 18-19 0.33016785825272876 1102 20-21 0.4688107751165873 1102 22-23 0.18880094234710043 1102 24-25 0.32109407528997025 1102 26-27 0.2151498200687243 1102 28-29 0.2894543685586015 1102 30-31 0.09166032289703452 1102 32-33 0.24405473709321512 1102 34-35 0.08044841564469607 1102 36-37 -0.009277721712471987 1102 38-39 -0.09533517059045948 1102 40-41 -0.010482616804644351 1102 42-43 0.20725613989536384 1102 44-45 0.17560653778418356 1102 46-47 0.21158305920044285 1102 48-49 0.45783774894129436 1102 50-51 0.41004276824892827 1102 52-53 0.24270389201719667 1102 54-55 0.37620929728347363 1102 56-57 0.28945857663156005 1102 58-59 0.15144232588126272 1102 60-61 0.17097972464210187 1102 62-63 0.2636405224436871 1102 64-65 0.25998873554807034 1102 66-67 0.28493130404247324 1102 68-69 0.26794059378224944 1102 70-71 -0.01873688906670168 1102 72-73 0.3220657363103143 1102 74-75 0.12752582214403674 1102 76-77 0.2092319832762719 1102 78-79 0.1426063877780237 1102 80-81 0.32772673249168705 1102 82-83 0.22253630732943463 1102 84-85 0.34468310104265143 1102 86-87 0.1796427282787576 1102 88-89 0.1970621304667688 1102 90-91 0.35764812896218245 1102 92-93 0.2769621906147357 1102 94-95 0.4205162822681068 1102 96-97 0.39468803636347616 1102 98-99 0.28653093593101886 1102 100-101 0.8511895319209408 1103 1 0.3236048411762731 1103 2 0.4095469292112739 1103 3 0.42646471464038527 1103 4 0.30713967222519045 1103 5 0.388767486906886 1103 6 0.38771478035717877 1103 7 0.3512682800294087 1103 8 0.28281288852390674 1103 9 0.41295803491640726 1103 10-11 0.268336819744313 1103 12-13 0.3379452225280417 1103 14-15 0.5833268165818382 1103 16-17 0.7210358608572491 1103 18-19 0.6778292981017628 1103 20-21 0.6103182437109993 1103 22-23 0.5084768541168572 1103 24-25 0.4849561408876255 1103 26-27 0.5702087312518032 1103 28-29 0.5562766547430513 1103 30-31 0.4461289685995311 1103 32-33 0.4620643081303726 1103 34-35 0.4939095599353749 1103 36-37 0.44195626915644937 1103 38-39 0.2916796160638526 1103 40-41 0.5135202433508184 1103 42-43 0.7571505977393187 1103 44-45 0.6380189472030722 1103 46-47 0.875838149764192 1103 48-49 0.9754367326542237 1103 50-51 1.002529640722237 1103 52-53 1.0316023311850202 1103 54-55 1.0814470689876075 1103 56-57 1.127191907904006 1103 58-59 0.8157499467126357 1103 60-61 0.8059782562587117 1103 62-63 0.8728781660142886 1103 64-65 0.8374015493044098 1103 66-67 0.8952384817827195 1103 68-69 0.8757898254178826 1103 70-71 0.8891274856226232 1103 72-73 0.9199161843184562 1103 74-75 0.9129199660384408 1103 76-77 0.7870571988351749 1103 78-79 0.8950881349100861 1103 80-81 1.0115945416972778 1103 82-83 0.8694508093346585 1103 84-85 0.7404416571480006 1103 86-87 0.5164001614236291 1103 88-89 0.466853216808385 1103 90-91 0.763535394303652 1103 92-93 0.7655796992553512 1103 94-95 0.7834109397046802 1103 96-97 0.6965594355480356 1103 98-99 0.82663897131469 1103 100-101 1.2426688312993335 1104 1 0.34183035463403044 1104 2 0.3542365646095966 1104 3 0.4647084899332192 1104 4 0.5295879223411362 1104 5 0.46032491931981667 1104 6 0.4372331701598 1104 7 0.3791423541890069 1104 8 0.44035849981430175 1104 9 0.5073367476590889 1104 10-11 0.5648014275766968 1104 12-13 0.6436616195006977 1104 14-15 0.6612484696531524 1104 16-17 0.5582379273888307 1104 18-19 0.5280720275244875 1104 20-21 0.7370031096722016 1104 22-23 0.5500541599862387 1104 24-25 0.5510676160166881 1104 26-27 0.22802988168967886 1104 28-29 0.10504985006386391 1104 30-31 0.1794159738317731 1104 32-33 0.21422327942523367 1104 34-35 0.5248638755206656 1104 36-37 0.45155720283846534 1104 38-39 0.45156279997343063 1104 40-41 0.7872546154342714 1104 42-43 0.9817538502318044 1104 44-45 0.9330887809222403 1104 46-47 1.182656342516033 1104 48-49 0.9928593219091866 1104 50-51 0.980922007506102 1104 52-53 0.8415354031951523 1104 54-55 1.1062852814445279 1104 56-57 1.163762242909577 1104 58-59 1.076385084292184 1104 60-61 1.1811170481296003 1104 62-63 0.9901031752991472 1104 64-65 0.8786499160343908 1104 66-67 0.93917837648371 1104 68-69 0.7920172651240023 1104 70-71 0.7830452746512861 1104 72-73 0.8726907468714877 1104 74-75 0.9481910215109259 1104 76-77 0.9972969359340276 1104 78-79 0.8076571940888222 1104 80-81 0.9894468950493511 1104 82-83 0.9741944124004931 1104 84-85 0.8234382337755441 1104 86-87 0.6599193748369423 1104 88-89 0.815038242488491 1104 90-91 0.7752343222627331 1104 92-93 0.8938188176840249 1104 94-95 0.9546778232426654 1104 96-97 0.8553655993503462 1104 98-99 0.5950602847428179 1104 100-101 1.2525984635802523 1105 1 0.3278077369667116 1105 2 0.28258594539634174 1105 3 0.4585709691387443 1105 4 0.40356920311275246 1105 5 0.3978784808462521 1105 6 0.3028882591349529 1105 7 0.42882224530157487 1105 8 0.40429163822568626 1105 9 0.2501658044721182 1105 10-11 0.48632328908129097 1105 12-13 0.36691866441676524 1105 14-15 0.5107573839715158 1105 16-17 0.6774669354713652 1105 18-19 0.5082059523451079 1105 20-21 0.37419098250243366 1105 22-23 0.3786257685964145 1105 24-25 0.2880637175777494 1105 26-27 0.22475317654272686 1105 28-29 0.3071290875157686 1105 30-31 0.1344484468199596 1105 32-33 0.19978792293651537 1105 34-35 0.007043640606113399 1105 36-37 0.29577087762128684 1105 38-39 -0.03710106048934847 1105 40-41 0.040916753526389016 1105 42-43 -0.02566599651921564 1105 44-45 0.23548560671914487 1105 46-47 0.35353756615536014 1105 48-49 0.40471175255225234 1105 50-51 0.5368286886238778 1105 52-53 0.6342375927063344 1105 54-55 0.4665968204877302 1105 56-57 0.4370268494528986 1105 58-59 0.4104147174692443 1105 60-61 0.20563352407522473 1105 62-63 0.3371461589492313 1105 64-65 0.3434116827665932 1105 66-67 0.32957258931831035 1105 68-69 0.3059642665186999 1105 70-71 0.5902794414439825 1105 72-73 0.4831440664258615 1105 74-75 0.568216100908014 1105 76-77 0.42244627807245294 1105 78-79 0.480361610079143 1105 80-81 0.4636113352368767 1105 82-83 0.28413711206946246 1105 84-85 0.2550590685791967 1105 86-87 0.5191985417136529 1105 88-89 0.38467032731314177 1105 90-91 0.32924639566092395 1105 92-93 0.41314717714697835 1105 94-95 0.19990034512937527 1105 96-97 0.4727504105617193 1105 98-99 0.7389885080964831 1105 100-101 1.1146764827699833 1106 1 0.23341196491796268 1106 2 -0.07861931304951142 1106 3 0.09600222382073298 1106 4 0.21305103242338674 1106 5 0.24591706958024417 1106 6 0.1256966309900207 1106 7 0.47847417930606895 1106 8 0.41038050924942127 1106 9 0.3848358269456682 1106 10-11 0.4072684572675058 1106 12-13 0.41316759098489086 1106 14-15 0.2761622733395157 1106 16-17 0.07348450321177324 1106 18-19 0.26498912824176557 1106 20-21 0.1287096739204614 1106 22-23 -0.10362606308947164 1106 24-25 -0.3390671588907921 1106 26-27 -0.29272891770002474 1106 28-29 -0.08258606477452446 1106 30-31 0.015684629239423487 1106 32-33 -0.16607191883505124 1106 34-35 -0.1215032523795827 1106 36-37 -0.4658701484882357 1106 38-39 -0.18825739784843165 1106 40-41 -0.13932616182322732 1106 42-43 -0.04929063337950623 1106 44-45 -0.3985894604274378 1106 46-47 -0.11404136534598308 1106 48-49 -0.056854818495231996 1106 50-51 -0.18977278037906586 1106 52-53 -0.09171068158063633 1106 54-55 0.04997050267881065 1106 56-57 0.18639535176420452 1106 58-59 -0.009952624622080464 1106 60-61 0.05216017259251515 1106 62-63 -0.10755382826094007 1106 64-65 -0.27050456214031726 1106 66-67 -0.21045728406240016 1106 68-69 -0.18569379746697479 1106 70-71 -0.3081864645325254 1106 72-73 -0.35390669091458804 1106 74-75 -0.22813217520607054 1106 76-77 -0.2883083673752793 1106 78-79 -0.19238555332002605 1106 80-81 -0.047286282201135066 1106 82-83 -0.06362169920750915 1106 84-85 0.20417953961024082 1106 86-87 0.20707195897260888 1106 88-89 0.2278510658342725 1106 90-91 0.4772224056004504 1106 92-93 0.46036447630102373 1106 94-95 0.6027296374865401 1106 96-97 0.5037714498264876 1106 98-99 0.46508295183037873 1106 100-101 1.1699490887885098 1107 1 0.13271247748070536 1107 2 0.22803113414396137 1107 3 0.2959829513650014 1107 4 0.2807961309364444 1107 5 0.41647769867186213 1107 6 0.23447665433446474 1107 7 0.2957359560452417 1107 8 0.30828482603784835 1107 9 0.5654682776528901 1107 10-11 0.5478410848221671 1107 12-13 0.36447292226571903 1107 14-15 0.2422065021150459 1107 16-17 0.2756644958652785 1107 18-19 0.5239766476652576 1107 20-21 0.27265331980993324 1107 22-23 0.25925690900111675 1107 24-25 0.13684695362167787 1107 26-27 0.08536222712010044 1107 28-29 0.15998875343606755 1107 30-31 -0.023153296053529004 1107 32-33 0.18732389994827514 1107 34-35 0.20369361062044788 1107 36-37 0.12390958969636046 1107 38-39 0.3714218154487128 1107 40-41 0.41009545305147554 1107 42-43 0.27303741281639304 1107 44-45 0.23197025035280916 1107 46-47 0.5182720482832082 1107 48-49 0.5563654215706961 1107 50-51 0.5293296669207379 1107 52-53 0.46297022938424703 1107 54-55 0.8048837705864855 1107 56-57 0.9456462815544668 1107 58-59 1.002301394802629 1107 60-61 0.6972485358958238 1107 62-63 0.7367899778496962 1107 64-65 0.580559380122061 1107 66-67 0.7163313991999232 1107 68-69 0.6125300856368199 1107 70-71 0.5880561315820501 1107 72-73 0.6075900841208757 1107 74-75 0.6279625425702626 1107 76-77 0.6481431264514903 1107 78-79 0.7373507886107937 1107 80-81 0.5690432272514307 1107 82-83 0.652410597561726 1107 84-85 0.7736002542981453 1107 86-87 0.7598206401083196 1107 88-89 0.7609794505970697 1107 90-91 0.6851862495068488 1107 92-93 0.6673165162813994 1107 94-95 0.5608455767136604 1107 96-97 0.7249619982275384 1107 98-99 0.6961794820633678 1107 100-101 1.0715265000204255 1108 1 0.003243181514072546 1108 2 0.12953623657992352 1108 3 0.1640669926463616 1108 4 0.26910706762456016 1108 5 0.13186604565722604 1108 6 0.22154982609652762 1108 7 0.35954104820169874 1108 8 0.3953272296956598 1108 9 -0.18285531831472923 1108 10-11 0.10685705466520545 1108 12-13 0.09503198646473976 1108 14-15 0.4838441960702724 1108 16-17 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-3.8515796944002645 2316 72-73 -3.0603731741448392 2316 74-75 -3.2201731879289106 2316 76-77 -3.658032749501068 2316 78-79 -3.942951726782816 2316 80-81 -4.729402280676773 2316 82-83 -3.9343368302643213 2316 84-85 -3.82326949385234 2316 86-87 -4.326387341218638 2316 88-89 -5.832545286181837 2316 90-91 -4.966670044604257 2316 92-93 -4.291787900622349 2316 94-95 -3.6542139086167182 2316 96-97 -5.472944639201941 2316 98-99 -7.533450154130371 2316 100-101 -8.221062191851722 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4061.0 3 1441.0 4 1408.0 5 1618.0 6 1625.0 7 1890.0 8 2412.0 9 2987.0 10 3697.0 11 4724.0 12 5827.0 13 6857.0 14 7826.0 15 9202.0 16 10966.0 17 13075.0 18 10832.0 19 9004.0 20 9176.0 21 9626.0 22 10801.0 23 12348.0 24 14041.0 25 16175.0 26 18912.0 27 22215.0 28 25974.0 29 31361.0 30 37518.0 31 45636.0 32 56478.0 33 71845.0 34 94612.0 35 127674.0 36 177805.0 37 226985.0 38 174944.0 39 26903.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.76417381609643 17.848947535212613 14.496332695707286 22.890545952983672 2 15.65105220172352 19.34486417171077 40.171130668417156 24.832952958148553 3 20.14675972388108 23.199941998237055 29.79313976516918 26.86015851271269 4 12.801972187876846 15.724153195646531 37.501717268855614 33.972157347621014 5 13.897416326374842 36.45864977292676 34.743273670953705 14.900660229744686 6 32.44641751705808 35.091822426518014 17.35149605025025 15.110264006173654 7 28.65870608468275 32.10358226189276 20.740355507494737 18.497356145929746 8 26.36019362621589 33.4513720280361 19.85959045306702 20.32884389268099 9 25.603720867723943 17.112417884416995 20.917205195225673 36.36665605263339 10-11 25.225951592627077 25.490508219770586 27.422038826834516 21.861501360767818 12-13 26.438499636634567 23.04496418254991 27.72588963461926 22.790646546196268 14-15 22.96297964640214 24.65897283005788 26.554463186167588 25.823584337372395 16-17 22.158536892653082 27.768390204718663 27.20285786060561 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22.195628385277164 25.954707299912855 28.693300184516207 23.156364130293777 44-45 22.427287111320705 25.69174107645331 28.88499972719909 22.995972085026892 46-47 22.97533955141593 26.115527223251956 28.52708818683855 22.38204503849356 48-49 22.804702865940087 26.12612578239611 28.382860445906168 22.686310905757633 50-51 22.261389724361933 26.429746308285928 28.821600824437976 22.487263142914156 52-53 22.808103253296867 26.602573396434604 27.91811587456867 22.671207475699855 54-55 22.24504668119055 26.250586614879225 28.307725765651647 23.196640938278577 56-57 22.76430581389803 26.2658081747508 27.949062812991844 23.020823198359327 58-59 23.112430591511227 26.039939761081797 27.970080472448938 22.87754917495804 60-61 22.622098258573587 26.170845988309093 28.84632407930282 22.360731673814502 62-63 22.234647912903664 26.424903678640355 28.72227016987204 22.618178238583944 64-65 22.070666912885752 26.818676429965677 28.29244265104702 22.818214006101552 66-67 22.30284586889445 26.422028289666716 27.931019089592084 23.34410675184675 68-69 22.267003499197028 26.225243591196417 28.37195956941295 23.135793340193604 70-71 22.76431573931029 26.161114330943956 27.95449975561914 23.12007017412661 72-73 22.460868483454824 26.038476303576157 28.517257248746624 22.983397964222398 74-75 22.108769814315917 27.039815613337502 27.89786993718948 22.9535446351571 76-77 22.323289682875878 26.442338882948235 27.868859340808637 23.36551209336725 78-79 22.447890848686267 26.500042548985714 28.01936570338053 23.032700898947496 80-81 22.87893126159059 26.409034365388873 27.676043439438917 23.03599093358162 82-83 22.579179253198475 26.246910838706576 28.216838995794124 22.95707091230082 84-85 22.416116601167538 26.582929528024724 27.924071883704073 23.07688198710367 86-87 22.427746370482968 26.768019577701292 27.765027904100094 23.03920614771565 88-89 22.291811573826248 26.589025044917246 27.398279466342302 23.720883914914204 90-91 22.299993246334417 26.330329033245203 27.767028299385032 23.60264942103534 92-93 22.66278931334062 26.395931144798617 28.05747956261867 22.88379997924209 94-95 22.2848950818934 26.261217104221334 28.092547572885184 23.361340241000082 96-97 21.868030097956876 26.21211664465553 28.53318822899571 23.38666502839189 98-99 22.108411802734146 26.695522950820923 27.604335617259125 23.59172962918581 100-101 22.00493737456691 26.18140367521097 27.877543341318056 23.936115608904064 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 133.0 1 102.0 2 113.5 3 165.5 4 150.0 5 135.5 6 237.5 7 479.0 8 793.5 9 1002.0 10 949.0 11 864.0 12 913.5 13 984.0 14 1114.5 15 1230.0 16 1310.5 17 1467.0 18 1641.5 19 1856.5 20 2106.5 21 2382.5 22 2884.5 23 3529.5 24 4334.0 25 5404.0 26 6878.0 27 8740.0 28 10560.5 29 12540.5 30 15025.0 31 17837.5 32 21136.5 33 23284.0 34 24507.5 35 26448.5 36 29130.0 37 31718.0 38 33315.0 39 34475.0 40 35667.5 41 37708.0 42 39164.0 43 40126.5 44 42008.5 45 44799.0 46 47042.0 47 51771.0 48 61334.5 49 60724.5 50 51015.5 51 46311.5 52 45174.5 53 44756.5 54 43927.0 55 41489.5 56 37984.5 57 34439.5 58 31901.0 59 28401.0 60 23644.5 61 19986.5 62 16676.0 63 13019.5 64 9413.0 65 6821.5 66 5228.0 67 4291.5 68 3352.0 69 2561.5 70 1990.5 71 1448.5 72 1101.5 73 900.0 74 707.0 75 543.5 76 402.0 77 275.0 78 184.5 79 111.0 80 78.5 81 54.0 82 37.0 83 32.5 84 22.0 85 11.0 86 8.5 87 5.0 88 3.0 89 5.0 90 6.5 91 4.0 92 1.0 93 0.0 94 1.5 95 2.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07203417960776473 2 0.028920502194219103 3 0.01404056043202194 4 0.02052668889246686 5 0.025868206448127377 6 0.03098080182283102 7 0.079130767217428 8 0.05715423784556757 9 0.06882926907436843 10-11 0.05726869893604601 12-13 0.03914569294362639 14-15 0.05310994598199604 16-17 0.06394559588062167 18-19 0.05875669311226572 20-21 0.01797039120511504 22-23 0.013506408676455887 24-25 0.021594992403598964 26-27 0.023998675303646196 28-29 0.0057230545239219865 30-31 8.775350270013712E-4 32-33 0.010148883355754989 34-35 0.012056568197062318 36-37 0.004616597315963735 38-39 0.009309502025579763 40-41 0.005150749071529788 42-43 0.0026707587778302603 44-45 7.24920239696785E-4 46-47 7.630739365229315E-4 48-49 8.775350270013712E-4 50-51 0.0015643015698720098 52-53 2.2892218095687947E-4 54-55 3.052295746091726E-4 56-57 0.003929830773093097 58-59 0.0030904494429178724 60-61 0.005265210162008227 62-63 0.0024418365968733808 64-65 2.2892218095687947E-4 66-67 8.01227633349078E-4 68-69 0.0034719864111793382 70-71 0.0026326050810041137 72-73 0.01739808575272284 74-75 0.015185171336806337 76-77 0.02350267724490629 78-79 0.017894083811462742 80-81 0.01213287559071461 82-83 0.004616597315963735 84-85 0.002975988352439433 86-87 0.0014116867825674233 88-89 0.0017932237508288891 90-91 0.006829511731880238 92-93 0.011064572079582507 94-95 0.01201841450023617 96-97 0.012781488436759103 98-99 0.02426575118142922 100-101 0.021251609132163642 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1310489.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.84159955079998 #Duplication Level Percentage of deduplicated Percentage of total 1 83.96137062269398 55.28150942275719 2 9.648980664113742 12.706086419199783 3 2.7339743874251567 5.400277403969728 4 1.1839328759399461 3.1180813725065963 5 0.6343617388554278 2.088369579003412 6 0.39174548274420323 1.5475889520407193 7 0.27159882964253096 1.251775096585263 8 0.19922935245081183 1.0494063394265236 9 0.1454597465366956 0.8619572144006981 >10 0.7648657377188792 8.75380114560487 >50 0.042683570161422775 1.8859235786873805 >100 0.017970954458553363 2.4052416219444535 >500 0.0023188104598238115 1.0746736215089476 >1k 0.0013912862758942868 2.172172034382519 >5k 1.1594052299119056E-4 0.40313619798186906 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5281 0.40297934587776013 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4665 0.35597399138794755 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4020 0.30675572248221844 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3263 0.24899102548743254 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3045 0.23235601367123265 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3039 0.23189816930931892 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2052 0.15658277177450555 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1837 0.14017668213926252 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1602 0.12224444463097364 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1499 0.11438478308478743 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.630739365229315E-5 0.0 5 0.0 0.0 0.0 7.630739365229315E-5 0.0 6 0.0 0.0 0.0 7.630739365229315E-5 7.630739365229315E-5 7 1.526147873045863E-4 0.0 0.0 7.630739365229315E-5 7.630739365229315E-5 8 2.2892218095687945E-4 0.0 0.0 7.630739365229315E-5 7.630739365229315E-5 9 2.2892218095687945E-4 0.0 0.0 7.630739365229315E-5 7.630739365229315E-5 10-11 2.2892218095687945E-4 0.0 0.0 7.630739365229315E-5 7.630739365229315E-5 12-13 3.052295746091726E-4 0.0 0.0 7.630739365229315E-5 7.630739365229315E-5 14-15 3.052295746091726E-4 0.0 0.0 7.630739365229315E-5 1.1446109047843972E-4 16-17 3.052295746091726E-4 0.0 0.0 7.630739365229315E-5 1.526147873045863E-4 18-19 3.815369682614657E-4 7.630739365229315E-5 0.0 7.630739365229315E-5 1.526147873045863E-4 20-21 4.578443619137589E-4 7.630739365229315E-5 0.0 7.630739365229315E-5 1.526147873045863E-4 22-23 4.578443619137589E-4 7.630739365229315E-5 0.0 1.526147873045863E-4 1.526147873045863E-4 24-25 4.578443619137589E-4 7.630739365229315E-5 0.0 4.959980587399055E-4 1.526147873045863E-4 26-27 4.578443619137589E-4 7.630739365229315E-5 0.0 7.24920239696785E-4 1.526147873045863E-4 28-29 6.486128460444918E-4 1.526147873045863E-4 0.0 0.0015643015698720095 1.526147873045863E-4 30-31 6.867665428706383E-4 1.526147873045863E-4 0.0 0.003433832714353192 1.526147873045863E-4 32-33 6.867665428706383E-4 1.526147873045863E-4 0.0 0.005684900827095839 1.526147873045863E-4 34-35 6.867665428706383E-4 1.526147873045863E-4 0.0 0.008203044817621514 1.526147873045863E-4 36-37 7.630739365229315E-4 1.526147873045863E-4 0.0 0.012361797771671491 1.526147873045863E-4 38-39 7.630739365229315E-4 1.526147873045863E-4 0.0 0.019611000168639342 1.526147873045863E-4 40-41 7.630739365229315E-4 1.526147873045863E-4 0.0 0.03098080182283102 1.526147873045863E-4 42-43 8.775350270013712E-4 1.526147873045863E-4 0.0 0.049561652177164406 1.526147873045863E-4 44-45 9.156887238275178E-4 1.526147873045863E-4 0.0 0.06665450835527806 1.526147873045863E-4 46-47 9.91996117479811E-4 1.526147873045863E-4 0.0 0.10644881414494894 1.526147873045863E-4 48-49 9.91996117479811E-4 1.526147873045863E-4 0.0 0.1468917327806643 1.526147873045863E-4 50-51 9.91996117479811E-4 1.526147873045863E-4 0.0 0.1748965462510559 1.526147873045863E-4 52-53 9.91996117479811E-4 1.526147873045863E-4 0.0 0.20404597062623187 1.526147873045863E-4 54-55 9.91996117479811E-4 1.526147873045863E-4 0.0 0.238689527344373 1.9076848413073286E-4 56-57 9.91996117479811E-4 1.526147873045863E-4 0.0 0.3074806427219152 2.6707587778302603E-4 58-59 9.91996117479811E-4 1.526147873045863E-4 0.0 0.39710367656653356 3.052295746091726E-4 60-61 9.91996117479811E-4 1.526147873045863E-4 0.0 0.4869556325921087 3.052295746091726E-4 62-63 9.91996117479811E-4 1.526147873045863E-4 0.0 0.5800506528479064 3.052295746091726E-4 64-65 0.0010683035111321041 1.526147873045863E-4 0.0 0.691879138245342 3.052295746091726E-4 66-67 0.0011446109047843973 1.526147873045863E-4 0.0 0.8465160714817141 3.052295746091726E-4 68-69 0.0011446109047843973 1.526147873045863E-4 0.0 1.0228243045153373 3.052295746091726E-4 70-71 0.0011827646016105437 1.9076848413073286E-4 0.0 1.1535388698417155 3.052295746091726E-4 72-73 0.001259071995262837 2.2892218095687945E-4 0.0 1.266893503112197 3.052295746091726E-4 74-75 0.0012972256920889835 2.2892218095687945E-4 0.0 1.4047046560482386 3.052295746091726E-4 76-77 0.0012972256920889835 2.2892218095687945E-4 0.0 1.5749464512865043 3.052295746091726E-4 78-79 0.0012972256920889835 2.2892218095687945E-4 0.0 1.7501100734153434 3.052295746091726E-4 80-81 0.0016787626603504493 2.2892218095687945E-4 0.0 1.9909743614788067 3.052295746091726E-4 82-83 0.0016787626603504493 2.2892218095687945E-4 0.0 2.2670545117128036 3.8153696826146577E-4 84-85 0.0017169163571765958 2.2892218095687945E-4 0.0 2.6463785655583525 3.8153696826146577E-4 86-87 0.0017550700540027424 2.2892218095687945E-4 0.0 3.0508459056123325 3.8153696826146577E-4 88-89 0.0017550700540027424 2.2892218095687945E-4 0.0 3.507583810318133 3.8153696826146577E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4380 0.0 23.972702 1 GAGTACT 4820 0.0 23.617815 12-13 TACATGG 4405 0.0 23.297358 2 TACCTGG 1020 0.0 22.824158 2 ACATGGG 4335 0.0 22.798498 3 GTACCTG 1135 0.0 22.185991 1 TAAGCGC 55 0.0028825824 21.585426 54-55 GTACTTT 5215 0.0 21.37158 14-15 CATGGGG 2610 0.0 21.118645 4 ACCGTAT 140 4.82688E-4 20.372965 8 AGAGTAC 7005 0.0 20.357647 10-11 AGTACTT 5095 0.0 20.010796 12-13 GTGTAGC 835 0.0 19.915005 1 GTACACG 170 7.940143E-5 19.563562 1 GTATCAA 11540 0.0 18.979902 1 ATACCGT 130 0.0075464672 18.276447 6 TCCGTAC 130 0.0075497916 18.275051 3 GTACGGG 130 0.007553117 18.273657 1 ACTTTTT 6305 0.0 18.08976 16-17 GCGACAG 345 1.0768417E-9 17.90562 7 >>END_MODULE