FastQCFastQC Report
Thu 26 May 2016
SRR936640_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936640_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310489
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT89440.6824933288261099No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41180.31423384706014323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37210.2839398117801828No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31370.23937629388724363No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27890.2128213208962456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20630.15742215310468077No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC20100.15337786124110922No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT18130.13834530469160747No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC17920.13674284942490933No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17160.13094348750733506No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG16380.12499151080245618No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA16020.12224444463097364No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14870.11346909436095992No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC14090.10751711765608105No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA13820.10545681802746913No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA13450.1026334444623343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG756.9331454E-637.9919135
ATACCGT1201.5089063E-731.6599256
TAAGGTG6400.028.9391525
GTAAGGT6000.027.7024364
GGTAAGG6800.023.0483233
GTACATG45600.023.024721
ACCGCTA1053.4457116E-822.62200260-61
TACATGG46100.022.5645772
ACATGGG44850.022.0259763
GAGTACT47700.021.80353512-13
ACCGTAT1353.801798E-421.1066178
CTAGGAC6200.020.6826573
AGGTAAG7400.020.5400872
GTACTTT51150.020.19364714-15
CATGGGG24000.019.9853294
TAGCCCT7150.019.9258274
TACCGTC1200.004769718719.7874537
GTGTAGC7800.019.4904691
AGTACTT51100.019.3769912-13
GGTGTGC12250.018.9959568
Produced by FastQC (version 0.11.5)