##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936640_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1310489 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87006529623675 31.0 31.0 34.0 30.0 34.0 2 32.02663585882827 31.0 31.0 34.0 30.0 34.0 3 32.10974453047679 33.0 31.0 34.0 30.0 34.0 4 35.803570270334205 37.0 35.0 37.0 35.0 37.0 5 35.62096591425033 37.0 35.0 37.0 33.0 37.0 6 35.71367252987244 37.0 35.0 37.0 33.0 37.0 7 35.65489218146814 37.0 35.0 37.0 33.0 37.0 8 35.62920100817328 37.0 35.0 37.0 33.0 37.0 9 37.26196175626045 39.0 37.0 39.0 33.0 39.0 10-11 37.14358151804403 39.0 37.0 39.0 33.0 39.0 12-13 37.0423773110648 39.0 37.0 39.0 33.0 39.0 14-15 38.173355518436246 40.0 37.0 41.0 33.0 41.0 16-17 38.082175432224155 40.0 37.0 41.0 33.0 41.0 18-19 38.217343678581045 40.0 37.5 41.0 33.0 41.0 20-21 38.22207397391355 40.0 38.0 41.0 33.0 41.0 22-23 38.13376686107247 40.0 37.0 41.0 33.0 41.0 24-25 37.98776143866908 40.0 37.0 41.0 33.0 41.0 26-27 37.76994656193222 40.0 37.0 41.0 32.5 41.0 28-29 37.692126374200775 40.0 37.0 41.0 32.5 41.0 30-31 37.68199084463891 40.0 37.0 41.0 33.0 41.0 32-33 37.53469887957854 40.0 36.5 41.0 32.0 41.0 34-35 37.35169009430831 39.0 36.0 41.0 31.5 41.0 36-37 37.13479853703465 39.0 36.0 41.0 31.0 41.0 38-39 36.96299854481801 39.0 36.0 41.0 31.0 41.0 40-41 36.79433326033259 39.0 35.5 41.0 30.5 41.0 42-43 36.52266558513654 39.0 35.0 40.5 30.0 41.0 44-45 36.25374840994469 39.0 35.0 40.0 30.0 41.0 46-47 35.90986036510036 38.5 35.0 40.0 29.0 41.0 48-49 35.53127992680595 38.0 35.0 40.0 27.5 41.0 50-51 35.23167001020229 38.0 34.0 40.0 27.0 41.0 52-53 34.87602948212461 38.0 34.0 40.0 26.0 41.0 54-55 34.68778028659531 38.0 34.0 40.0 26.0 41.0 56-57 34.361127411218256 38.0 33.5 40.0 25.5 41.0 58-59 33.97812686714654 37.0 33.0 40.0 24.0 41.0 60-61 33.53814606608678 36.5 32.5 39.5 23.0 41.0 62-63 33.215468424382045 36.0 32.0 39.0 22.5 40.0 64-65 32.793251984564534 36.0 31.5 39.0 22.0 40.0 66-67 32.358282290045935 35.0 31.0 38.5 21.0 40.0 68-69 31.98424481243261 35.0 31.0 38.0 20.0 40.0 70-71 31.59651168380658 35.0 31.0 37.5 18.5 39.5 72-73 31.142563958949673 34.5 30.0 37.0 18.0 39.0 74-75 30.65297228744385 34.0 30.0 36.0 15.5 39.0 76-77 29.149030629024736 32.5 28.0 35.0 12.5 37.0 78-79 29.939718303625593 34.0 29.0 35.0 11.5 37.0 80-81 29.709237925690335 34.0 29.0 35.0 7.0 37.0 82-83 29.41427474782314 34.0 29.0 35.0 7.0 36.5 84-85 29.05299968179817 34.0 29.0 35.0 3.5 36.0 86-87 28.690575426424793 33.5 29.0 35.0 2.0 36.0 88-89 28.219144151534273 33.0 27.5 35.0 2.0 35.0 90-91 27.735588394866344 33.0 27.0 35.0 2.0 35.0 92-93 27.089648215284523 32.5 25.5 34.5 2.0 35.0 94-95 26.542723365095014 32.0 25.0 34.0 2.0 35.0 96-97 25.851840038336835 32.0 24.0 34.0 2.0 35.0 98-99 25.0020652596092 32.0 20.0 34.0 2.0 35.0 100-101 21.859797754883864 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3683186127261848 1101 2 0.3291900724092258 1101 3 0.34011396556331164 1101 4 0.2607745911652444 1101 5 0.3292504246881123 1101 6 0.3363548382248993 1101 7 0.3142348848397347 1101 8 0.289541105640275 1101 9 0.32588112522119417 1101 10-11 0.47916669433627845 1101 12-13 0.4107271361761846 1101 14-15 0.6302235434390226 1101 16-17 0.5500474815471037 1101 18-19 0.5183575416070383 1101 20-21 0.4725120942865999 1101 22-23 0.40303923674838416 1101 24-25 0.37620149484456533 1101 26-27 0.36392782313966876 1101 28-29 0.4088738637215741 1101 30-31 0.46432097555307905 1101 32-33 0.41733738151095423 1101 34-35 0.4596181366807528 1101 36-37 0.4251539909285569 1101 38-39 0.5297912036259405 1101 40-41 0.6737469902584081 1101 42-43 0.6383164526887768 1101 44-45 0.6111777561663416 1101 46-47 0.5248808602700663 1101 48-49 0.41425093655612244 1101 50-51 0.3546088474100273 1101 52-53 0.5318356034411735 1101 54-55 0.3861961462815202 1101 56-57 0.45585725333877036 1101 58-59 0.21734344951827467 1101 60-61 0.35375366645249073 1101 62-63 0.4642157363274535 1101 64-65 0.3748066527427696 1101 66-67 0.1700222492220007 1101 68-69 0.4617421877167516 1101 70-71 0.4283909906798442 1101 72-73 0.44915150772281365 1101 74-75 0.18408955022809437 1101 76-77 0.18682405308925354 1101 78-79 0.3195548760726261 1101 80-81 0.45077763816383154 1101 82-83 0.45103525395453303 1101 84-85 0.49216889737322944 1101 86-87 0.42491807080318367 1101 88-89 0.2555438331733093 1101 90-91 0.49527686557891926 1101 92-93 0.518833696578568 1101 94-95 0.545287529353498 1101 96-97 0.6774664455366697 1101 98-99 0.49521458987180367 1101 100-101 1.0547661744365122 1102 1 0.27627900203300015 1102 2 0.25102466605307683 1102 3 0.22295249216060142 1102 4 0.24444449769630694 1102 5 0.2682049717773012 1102 6 0.24707385273073612 1102 7 0.1817378353721395 1102 8 0.2971395847674998 1102 9 0.22262785675053465 1102 10-11 0.2916105851812958 1102 12-13 0.26969397578818644 1102 14-15 0.5186433984787939 1102 16-17 0.5216877160204305 1102 18-19 0.3187688180351458 1102 20-21 0.21671646401692612 1102 22-23 0.2793042060007309 1102 24-25 0.35368198536853157 1102 26-27 0.44481360402544823 1102 28-29 0.34005894792208124 1102 30-31 0.47365119652425136 1102 32-33 0.44621729075551997 1102 34-35 0.3492629939272902 1102 36-37 0.4019927202216991 1102 38-39 0.3206058121123547 1102 40-41 0.27231438826479604 1102 42-43 0.4620433102164796 1102 44-45 0.479222100788526 1102 46-47 0.4247579350419599 1102 48-49 0.5200562112253593 1102 50-51 0.339768895403175 1102 52-53 0.38395295780257044 1102 54-55 0.2116387261420556 1102 56-57 0.21045697806471964 1102 58-59 0.34420980429487 1102 60-61 0.45894366112355556 1102 62-63 0.49708025376420295 1102 64-65 0.1962835862814174 1102 66-67 0.022719248342561116 1102 68-69 0.16645486634246964 1102 70-71 0.06834299753226958 1102 72-73 0.24704510547615754 1102 74-75 0.17062498767517198 1102 76-77 0.09338812949905062 1102 78-79 0.2144433443131284 1102 80-81 0.2874538184585269 1102 82-83 0.2663250599876754 1102 84-85 0.2899173387401852 1102 86-87 0.24647640961257267 1102 88-89 0.20789886477097852 1102 90-91 0.19751469320243942 1102 92-93 0.28239780895053457 1102 94-95 0.07379604748333435 1102 96-97 0.38175117436557926 1102 98-99 0.3779272488817931 1102 100-101 1.1292814207610142 1103 1 0.15887777405678705 1103 2 0.2009871780347936 1103 3 0.24481874808314075 1103 4 0.20455800787630807 1103 5 0.23314589034687572 1103 6 0.1802793213444005 1103 7 0.18056223262372129 1103 8 0.0709404442030035 1103 9 0.2129752800681075 1103 10-11 0.19098700946278058 1103 12-13 0.15966053786473822 1103 14-15 0.27893333867492487 1103 16-17 0.14169664056569076 1103 18-19 0.16710564703938502 1103 20-21 0.07253465597663222 1103 22-23 -0.08001581712602501 1103 24-25 0.1256971517192369 1103 26-27 0.048151728925617476 1103 28-29 0.003961223255508628 1103 30-31 0.145643917106014 1103 32-33 0.022025075225961643 1103 34-35 0.12014523137172262 1103 36-37 0.09961225906195637 1103 38-39 0.12690364237709417 1103 40-41 0.14168090907876518 1103 42-43 0.15933557092878914 1103 44-45 0.19257988701743756 1103 46-47 0.2503679749761645 1103 48-49 0.2134374871082727 1103 50-51 0.32417647830971674 1103 52-53 0.1770506822451381 1103 54-55 0.08057862053941278 1103 56-57 0.08348471292597281 1103 58-59 0.11666414160369953 1103 60-61 0.05923850265126873 1103 62-63 0.06158339023559023 1103 64-65 0.026305720676916167 1103 66-67 -0.13653460232766435 1103 68-69 -0.049512856552190954 1103 70-71 -0.02028127620037523 1103 72-73 -0.19968449894740203 1103 74-75 -0.10966868004187447 1103 76-77 -0.03724938186104154 1103 78-79 -0.15156370347910908 1103 80-81 -0.07328239380228396 1103 82-83 0.05233567849405674 1103 84-85 0.3040413724464699 1103 86-87 0.3152048164804384 1103 88-89 0.12018765445345281 1103 90-91 0.0477468680249622 1103 92-93 0.08298406465802088 1103 94-95 -0.020770306757047052 1103 96-97 0.03235959582991299 1103 98-99 -0.054210589644416274 1103 100-101 0.3732864179687354 1104 1 0.26076110998796054 1104 2 0.2870249082561074 1104 3 0.329062767765123 1104 4 0.22351716987168402 1104 5 0.24004675686998667 1104 6 0.09323901830185122 1104 7 0.15485470678486024 1104 8 0.21973952168205813 1104 9 0.2626793671399241 1104 10-11 0.39086137515746344 1104 12-13 0.37579580244967303 1104 14-15 0.5576416073028625 1104 16-17 0.48736212473523466 1104 18-19 0.3391571737018566 1104 20-21 0.37876472719586474 1104 22-23 0.32180661394949794 1104 24-25 0.4011435708408513 1104 26-27 0.3918517566358801 1104 28-29 0.3605680530663662 1104 30-31 0.3755469985260902 1104 32-33 0.37114772779117544 1104 34-35 0.38154781342971233 1104 36-37 0.40930563245971996 1104 38-39 0.4176362902662092 1104 40-41 0.31057401865169965 1104 42-43 0.4207058002889781 1104 44-45 0.5471941995623055 1104 46-47 0.5427498888318709 1104 48-49 0.5601323643652663 1104 50-51 0.5642284446802321 1104 52-53 0.7620389342469167 1104 54-55 0.6387577914636466 1104 56-57 0.5748405290991556 1104 58-59 0.7467670881286637 1104 60-61 0.7347956666209967 1104 62-63 0.5301437841832097 1104 64-65 0.6269645851717911 1104 66-67 0.50783925149684 1104 68-69 0.5923650287311517 1104 70-71 0.5560828771066753 1104 72-73 0.42327667973955485 1104 74-75 0.39710770677034546 1104 76-77 0.3365902507470153 1104 78-79 0.3964846230324959 1104 80-81 0.6885205679248365 1104 82-83 0.57531980474511 1104 84-85 0.7871186263590992 1104 86-87 0.8508662691911404 1104 88-89 0.7592983617772369 1104 90-91 0.8121499523315201 1104 92-93 0.7585288407917865 1104 94-95 0.8440992096410831 1104 96-97 0.9583943529705969 1104 98-99 1.0400793636522359 1104 100-101 1.6278820645704748 1105 1 0.05596283739969721 1105 2 0.09595939095678574 1105 3 0.06598955440961163 1105 4 0.17537480963575547 1105 5 0.2202668002233139 1105 6 0.11045852393946376 1105 7 0.04908434500300274 1105 8 0.14205010291914277 1105 9 0.07953465439774021 1105 10-11 0.17546842259407214 1105 12-13 0.18399302649228844 1105 14-15 0.17528162343433706 1105 16-17 0.21513304103643094 1105 18-19 0.1341677599800235 1105 20-21 0.21865777217914228 1105 22-23 0.24400587122966044 1105 24-25 0.3725900261180328 1105 26-27 0.16700676999476372 1105 28-29 0.08399943549986943 1105 30-31 0.14881895665147482 1105 32-33 0.0982013397284689 1105 34-35 0.17134507780099995 1105 36-37 0.2833499267918569 1105 38-39 0.14674729495441596 1105 40-41 0.22798424533248607 1105 42-43 0.3276694288618387 1105 44-45 0.25596652736724224 1105 46-47 0.28573647954619474 1105 48-49 0.2712046871291278 1105 50-51 0.2960280279505483 1105 52-53 0.21625958253042654 1105 54-55 0.153834836043643 1105 56-57 0.12123321280468247 1105 58-59 0.11701586566558575 1105 60-61 0.2069027140576054 1105 62-63 0.2544657582720333 1105 64-65 0.3224116453791481 1105 66-67 0.14170105855771453 1105 68-69 0.17013111399269576 1105 70-71 0.21628855336820152 1105 72-73 0.25670873431769436 1105 74-75 0.33994180466821433 1105 76-77 0.2839518729681174 1105 78-79 0.3845635076084548 1105 80-81 0.3625220130358251 1105 82-83 0.6861154979782924 1105 84-85 0.7675308610720144 1105 86-87 0.7197224470406134 1105 88-89 0.5656786948249071 1105 90-91 0.5526517083094085 1105 92-93 0.5068852370882695 1105 94-95 0.7812337309900776 1105 96-97 0.8979906040838408 1105 98-99 0.8227960042733002 1105 100-101 1.0996519894583123 1106 1 0.05107301262032138 1106 2 0.08908029136058104 1106 3 0.02666540538985629 1106 4 0.06844272361504267 1106 5 0.10159776439353152 1106 6 -0.011647777790813052 1106 7 -0.04845635405237658 1106 8 -0.007926601325252136 1106 9 -0.022989509238954042 1106 10-11 -0.05409640355563994 1106 12-13 -8.165421251362659E-4 1106 14-15 0.045281029620959146 1106 16-17 0.09189050024601642 1106 18-19 0.11815306995060126 1106 20-21 0.021096061265765798 1106 22-23 -0.06111371364910667 1106 24-25 -0.0634791120303646 1106 26-27 -0.09849061722637487 1106 28-29 -0.17059919233445697 1106 30-31 -0.20005782250476045 1106 32-33 -0.11070106475893482 1106 34-35 -0.010545669673007296 1106 36-37 -0.22369361343214678 1106 38-39 -0.029077420471942617 1106 40-41 0.0759543171382262 1106 42-43 -0.07261902740608406 1106 44-45 -0.2525626882509613 1106 46-47 -0.15993530609631534 1106 48-49 -0.2894088134464212 1106 50-51 -0.14679687932320462 1106 52-53 -0.23035202697789714 1106 54-55 -0.3794438360069279 1106 56-57 -0.5856913363539249 1106 58-59 -0.49704769063705356 1106 60-61 -0.30595905532354806 1106 62-63 -0.20877148387592825 1106 64-65 -0.19705858173163193 1106 66-67 -0.3205518237210683 1106 68-69 -0.4464572911159195 1106 70-71 -0.15481968510724897 1106 72-73 -0.08515990502746362 1106 74-75 -0.18236959106729245 1106 76-77 -0.12508505308841222 1106 78-79 -0.2845122903853685 1106 80-81 -0.27688252917974054 1106 82-83 -0.1636634207332257 1106 84-85 0.12532626038867178 1106 86-87 0.14672108583764043 1106 88-89 -0.09763482950316771 1106 90-91 -0.12170446095481324 1106 92-93 -0.09315518789939858 1106 94-95 0.013642009662124366 1106 96-97 0.1418092352069671 1106 98-99 0.13074990608397385 1106 100-101 0.6767620824586196 1107 1 0.03102089845005196 1107 2 0.04343266434965898 1107 3 -0.021409403154301287 1107 4 0.12183679035694439 1107 5 -0.030163303070942504 1107 6 -0.02857280008402796 1107 7 0.003685003066827619 1107 8 -0.03313334221402897 1107 9 0.04306849420712666 1107 10-11 0.1617261013561162 1107 12-13 0.04756200394630383 1107 14-15 0.2176210380307566 1107 16-17 0.1602116578983228 1107 18-19 0.06031893752527395 1107 20-21 0.12002764827697376 1107 22-23 -0.04025535834476557 1107 24-25 0.03288845297037568 1107 26-27 0.011959886999065361 1107 28-29 -0.15909749345841817 1107 30-31 0.019530514215851724 1107 32-33 0.15204080758128669 1107 34-35 0.12104759451309377 1107 36-37 0.09816802725194407 1107 38-39 -0.09705557070284243 1107 40-41 0.11817186993508955 1107 42-43 0.12915678741401138 1107 44-45 0.10586843718795791 1107 46-47 0.006427371312987873 1107 48-49 0.16109737548890024 1107 50-51 0.06573057939507265 1107 52-53 0.16949554541766076 1107 54-55 0.012568335243642537 1107 56-57 -0.03307300105911537 1107 58-59 0.07060769127172506 1107 60-61 0.17292657987000837 1107 62-63 0.23145928414653838 1107 64-65 0.06573419043289874 1107 66-67 0.013093805962618887 1107 68-69 0.0270974559497148 1107 70-71 0.02300285363703125 1107 72-73 0.2487926339826423 1107 74-75 0.18670563917511984 1107 76-77 0.15163765675177387 1107 78-79 0.21507733922098637 1107 80-81 0.5095138659648093 1107 82-83 0.42076911721357746 1107 84-85 0.6562312139535287 1107 86-87 0.43219448743211686 1107 88-89 0.25762328849017635 1107 90-91 0.4433776856221847 1107 92-93 0.7349477983041837 1107 94-95 0.6692633137609363 1107 96-97 0.7333769618788395 1107 98-99 0.6995462294552404 1107 100-101 1.2295661945602738 1108 1 0.05575789100515749 1108 2 0.13154420682442947 1108 3 -0.012049774005006952 1108 4 0.03792365075705817 1108 5 0.06396456550912433 1108 6 -0.0016404997704384527 1108 7 -0.03453644561098912 1108 8 -0.03191212306486335 1108 9 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-1.9880273739470198 2316 60-61 -1.5366582434950473 2316 62-63 -1.6582430698783526 2316 64-65 -1.4406873275230225 2316 66-67 -1.4657773928226376 2316 68-69 -2.055090485881337 2316 70-71 -1.8244540825815605 2316 72-73 -1.4203190804026988 2316 74-75 -1.6283506116916513 2316 76-77 -1.481318292955418 2316 78-79 -1.8900628880687584 2316 80-81 -1.4570535218690885 2316 82-83 -1.8296177999571306 2316 84-85 -1.6726311011337351 2316 86-87 -1.6597223221638728 2316 88-89 -1.6372608534664401 2316 90-91 -1.5705497817529341 2316 92-93 -1.482738470245799 2316 94-95 -1.5246578441947598 2316 96-97 -1.9011736226950724 2316 98-99 -2.6047786914665565 2316 100-101 -2.998109692690754 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 21.0 5 44.0 6 97.0 7 260.0 8 650.0 9 1116.0 10 1793.0 11 2708.0 12 3789.0 13 5100.0 14 6496.0 15 8438.0 16 10270.0 17 12600.0 18 11663.0 19 9045.0 20 8401.0 21 8648.0 22 9280.0 23 10382.0 24 12187.0 25 14477.0 26 17898.0 27 22269.0 28 27516.0 29 33678.0 30 41263.0 31 50417.0 32 62538.0 33 79062.0 34 102776.0 35 139830.0 36 196174.0 37 245794.0 38 145625.0 39 8173.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.450711857576586 19.25694188976931 14.679362617669819 22.612983634984285 2 16.079264560658025 20.84731818924162 38.55355760997477 24.519859640125585 3 20.748256231016956 23.709839893771274 29.347288572780418 26.194615302431355 4 13.428117290568636 16.67041844685457 36.61030348213529 33.2911607804415 5 13.845633763264608 37.61219496933096 33.860483714655054 14.681687552749379 6 32.62865998875229 36.198396171200216 16.406089635243028 14.766854204804467 7 29.05955644004371 32.72872395626668 19.911513878799347 18.30020572489027 8 26.825101164527133 34.1628964455253 18.90500416256832 20.106998227379243 9 25.891709125372287 17.498811512343863 19.91203283659764 36.69744652568621 10-11 25.56213749218803 26.20250913971807 26.561153889883855 21.674199478210042 12-13 26.797420782540684 23.564547206165702 27.001774165855892 22.636257845437726 14-15 22.9820432756142 25.574673174186024 25.851670069989407 25.591613480210363 16-17 22.30150993428018 28.445521227787367 26.562977517908408 22.689991320024035 18-19 22.258354117757932 27.482751502983838 28.686016117038342 21.572878262219888 20-21 23.121750735794045 25.889534364653194 28.81081031584393 22.17790458370883 22-23 22.425386573551226 25.967883719652523 28.942691577488695 22.664038129307556 24-25 23.057728832519768 26.095106483152474 28.83458006896662 22.012584615361135 26-27 22.612176656175492 25.984563512306497 29.283274314909995 22.11998551660802 28-29 22.04471746346675 27.255484757144494 28.79052997062078 21.90926780876798 30-31 22.443275144308856 26.27083173562472 28.59263142512556 22.693261694940865 32-33 22.40860178948296 26.377302435899246 28.744481079321737 22.469614695296052 34-35 23.09831877369375 26.24619221166114 28.67253172902588 21.98295728561923 36-37 23.23142911866577 25.881872200928058 28.944302045282676 21.942396635123494 38-39 22.665116080313048 26.600339419690005 28.791534653163136 21.943009846833814 40-41 22.658842076667856 26.569916153115948 28.557502418953607 22.21373935126259 42-43 22.512875652749234 26.798260708979342 27.847085550499497 22.841778087771928 44-45 22.866692357293022 26.355186899306737 28.056758478860555 22.721362264539685 46-47 23.60170883494527 27.101409517937462 27.4111043748357 21.88577727228157 48-49 23.36650065834832 27.013952558477776 27.2921719695088 22.327374813665106 50-51 22.72939546184322 27.29464138549914 27.889839510167203 22.08612364249043 52-53 23.376726936973714 27.59215099753145 26.797368913036014 22.23375315245882 54-55 22.62691286916257 27.335352462406505 27.21096922774036 22.826765440690565 56-57 23.186180905673165 27.215605285612014 27.026331896992538 22.57188191172228 58-59 23.61749938555528 26.8344800423776 26.847875700505142 22.70014487156197 60-61 22.834437126530037 27.33485271859799 27.8240606817847 22.006649473087265 62-63 22.500168856587145 27.32202611114312 27.767960139923918 22.409844892345816 64-65 22.45582997066907 27.90124719417564 27.16228788883707 22.48063494631822 66-67 22.700420202214445 27.294409780528 26.99565527774821 23.00951473950935 68-69 22.678933008360744 27.18449380164895 27.119669706162565 23.01690348382774 70-71 23.23184187875051 27.28117312024223 26.830348694532113 22.656636306475146 72-73 22.988289104296182 26.993702350801875 27.418772687141974 22.599235857759968 74-75 22.549530582172732 28.113255492566314 26.694814693850144 22.642399231410817 76-77 22.86356924556162 27.272796645414342 26.852786648810138 23.010847460213895 78-79 22.880631898270483 27.613687313383 26.709569099564046 22.79611168878247 80-81 23.355051768588382 27.377020028177746 26.52352139356186 22.744406809672018 82-83 22.98067338617966 27.47974758321923 26.9217089727327 22.61787005786841 84-85 22.646442995643675 27.94655009087446 26.757396097397883 22.649610816083978 86-87 22.695133156686527 27.85510739230243 26.453978216274297 22.99578123473674 88-89 22.310713631366802 28.03789544399658 26.013038964211553 23.638351960425062 90-91 22.61202661383599 27.565437131874383 26.348613767277808 23.473922487011823 92-93 22.884960345630386 27.73222041570298 26.558580839191798 22.824238399474837 94-95 22.46394871620675 27.63247937950152 26.68602225803952 23.217549646252206 96-97 22.11236555548133 27.551148564175488 27.240678354313197 23.095807526029986 98-99 22.18688441980851 27.895307465603857 26.158130621344693 23.759677493242933 100-101 21.669241147818997 27.86994836020949 26.367904682857773 24.09290580911374 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 211.0 1 151.5 2 121.5 3 158.0 4 151.5 5 144.5 6 220.0 7 463.0 8 719.5 9 877.5 10 922.5 11 884.0 12 905.0 13 1031.5 14 1183.5 15 1311.0 16 1480.5 17 1623.0 18 1762.0 19 1940.0 20 2150.0 21 2480.5 22 2970.5 23 3553.5 24 4339.0 25 5381.0 26 6776.0 27 8620.5 28 10522.5 29 12667.5 30 15197.0 31 17850.5 32 20830.5 33 22655.0 34 24284.0 35 26437.5 36 29058.5 37 31699.5 38 33115.5 39 34287.0 40 35552.5 41 37445.0 42 39068.5 43 40233.0 44 41687.5 45 43889.5 46 45491.5 47 50981.0 48 62544.5 49 61454.5 50 50482.5 51 45760.0 52 44546.5 53 44328.0 54 44198.5 55 42187.0 56 38050.0 57 34474.5 58 32602.5 59 29410.0 60 24295.5 61 20092.5 62 16909.0 63 13187.0 64 9165.5 65 6568.0 66 5201.5 67 4424.0 68 3565.5 69 2858.5 70 2238.5 71 1578.0 72 1197.0 73 955.5 74 758.0 75 609.5 76 463.0 77 295.0 78 205.0 79 152.0 80 114.5 81 88.0 82 59.0 83 39.5 84 24.0 85 11.5 86 5.0 87 4.5 88 2.5 89 1.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019458385381334754 2 0.01091195729227792 3 0.008317505908099954 4 0.0 5 0.0035864475016577782 6 0.0 7 7.630739365229315E-5 8 0.0 9 0.0 10-11 0.0 12-13 0.0010683035111321041 14-15 3.8153696826146577E-4 16-17 1.1446109047843974E-4 18-19 9.538424206536643E-4 20-21 0.0 22-23 7.630739365229315E-5 24-25 0.0 26-27 0.001716916357176596 28-29 0.002975988352439433 30-31 0.0014116867825674233 32-33 0.008431966998578394 34-35 0.0012972256920889835 36-37 7.630739365229315E-4 38-39 0.0012972256920889835 40-41 3.8153696826146577E-4 42-43 0.005341517555660521 44-45 0.0018313774476550358 46-47 4.1969066508761233E-4 48-49 9.538424206536643E-4 50-51 7.630739365229315E-5 52-53 3.052295746091726E-4 54-55 0.0017550700540027424 56-57 0.016406089635243027 58-59 0.027699583895782413 60-61 0.023655292032210874 62-63 0.015681169395546243 64-65 0.020450381498814566 66-67 0.003700908592136218 68-69 0.0014879941762197164 70-71 7.630739365229315E-5 72-73 0.0 74-75 0.0027470661714825537 76-77 0.0033575253207008987 78-79 0.011560570138322412 80-81 0.01793223750828889 82-83 0.020984533254380617 84-85 0.033575253207008986 86-87 0.011675031228800852 88-89 0.04341890698815481 90-91 0.04914196151207679 92-93 0.03197279794031083 94-95 0.0339949438720966 96-97 0.05352963664708364 98-99 0.05715423784556757 100-101 0.06409821066792624 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1310489.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.60322237407288 #Duplication Level Percentage of deduplicated Percentage of total 1 82.06769363983076 51.37702074661611 2 10.678354630941158 13.3699881910004 3 3.120773425896344 5.861114182814581 4 1.309479048667992 3.2791043231180677 5 0.7341906438741839 2.2981350071709636 6 0.46146645970781647 1.7333572437158742 7 0.3060786842390814 1.341305835336799 8 0.22154408853999613 1.1095499072424533 9 0.16156672122257806 0.9103137639252206 >10 0.8634536732470184 9.658321172363141 >50 0.04844785108010014 2.0740409797095185 >100 0.022702108667335682 2.768415410555728 >500 0.0018210103223708994 0.7752835770094705 >1k 0.002306613075003139 2.764299016075724 >5k 1.2140068815805996E-4 0.6797506433460155 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8944 0.6824933288261099 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4118 0.31423384706014323 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3721 0.2839398117801828 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3137 0.23937629388724363 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2789 0.2128213208962456 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2063 0.15742215310468077 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2010 0.15337786124110922 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1813 0.13834530469160747 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1792 0.13674284942490933 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1716 0.13094348750733506 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1638 0.12499151080245618 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1602 0.12224444463097364 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1487 0.11346909436095992 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1409 0.10751711765608105 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1382 0.10545681802746913 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1345 0.1026334444623343 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 7.630739365229315E-5 7.630739365229315E-5 9 0.0 0.0 0.0 2.2892218095687945E-4 7.630739365229315E-5 10-11 0.0 0.0 0.0 2.2892218095687945E-4 7.630739365229315E-5 12-13 7.630739365229315E-5 0.0 0.0 3.052295746091726E-4 7.630739365229315E-5 14-15 7.630739365229315E-5 0.0 0.0 3.8153696826146577E-4 7.630739365229315E-5 16-17 7.630739365229315E-5 0.0 0.0 3.8153696826146577E-4 7.630739365229315E-5 18-19 1.1446109047843972E-4 0.0 0.0 3.8153696826146577E-4 7.630739365229315E-5 20-21 1.526147873045863E-4 0.0 0.0 3.8153696826146577E-4 7.630739365229315E-5 22-23 1.526147873045863E-4 0.0 0.0 5.341517555660521E-4 7.630739365229315E-5 24-25 3.052295746091726E-4 0.0 0.0 8.775350270013712E-4 7.630739365229315E-5 26-27 3.052295746091726E-4 0.0 0.0 0.0011064572079582508 7.630739365229315E-5 28-29 4.1969066508761233E-4 0.0 0.0 0.0017550700540027424 7.630739365229315E-5 30-31 4.578443619137589E-4 0.0 0.0 0.0037390622889623645 7.630739365229315E-5 32-33 4.578443619137589E-4 0.0 0.0 0.006409821066792625 7.630739365229315E-5 34-35 4.578443619137589E-4 0.0 0.0 0.009195040935101324 7.630739365229315E-5 36-37 4.578443619137589E-4 0.0 0.0 0.013506408676455889 7.630739365229315E-5 38-39 5.341517555660521E-4 0.0 0.0 0.02094637955755447 7.630739365229315E-5 40-41 5.341517555660521E-4 0.0 0.0 0.032621410786355325 7.630739365229315E-5 42-43 5.723054523921986E-4 0.0 0.0 0.05104964635338412 7.630739365229315E-5 44-45 6.867665428706383E-4 0.0 0.0 0.06856219319658541 7.630739365229315E-5 46-47 6.867665428706383E-4 0.0 0.0 0.10816573050212555 7.630739365229315E-5 48-49 6.867665428706383E-4 0.0 0.0 0.1484178806537102 7.630739365229315E-5 50-51 6.867665428706383E-4 0.0 0.0 0.1766134626082325 7.630739365229315E-5 52-53 6.867665428706383E-4 0.0 0.0 0.2061062702548438 7.630739365229315E-5 54-55 6.867665428706383E-4 0.0 0.0 0.24029198261107115 7.630739365229315E-5 56-57 7.630739365229315E-4 0.0 0.0 0.30881602211083037 7.630739365229315E-5 58-59 7.630739365229315E-4 0.0 0.0 0.39840090225862257 7.630739365229315E-5 60-61 7.630739365229315E-4 0.0 0.0 0.48775686022545783 7.630739365229315E-5 62-63 7.630739365229315E-4 0.0 0.0 0.5811571100558646 7.630739365229315E-5 64-65 7.630739365229315E-4 0.0 0.0 0.6922225215167773 7.630739365229315E-5 66-67 8.393813301752247E-4 0.0 0.0 0.8460582271198003 7.630739365229315E-5 68-69 8.393813301752247E-4 0.0 0.0 1.0216033862169007 7.630739365229315E-5 70-71 9.156887238275178E-4 0.0 0.0 1.1510588795480161 7.630739365229315E-5 72-73 9.156887238275178E-4 0.0 0.0 1.2644135128184975 1.1446109047843972E-4 74-75 9.156887238275178E-4 0.0 0.0 1.4007366715783194 1.526147873045863E-4 76-77 9.156887238275178E-4 0.0 0.0 1.5703298539705408 1.526147873045863E-4 78-79 9.156887238275178E-4 0.0 0.0 1.7446159410723783 1.526147873045863E-4 80-81 9.91996117479811E-4 0.0 0.0 1.9843737719278836 1.526147873045863E-4 82-83 9.91996117479811E-4 0.0 0.0 2.25846992992692 1.526147873045863E-4 84-85 9.91996117479811E-4 0.0 0.0 2.6342838436644644 1.526147873045863E-4 86-87 9.91996117479811E-4 0.0 0.0 3.032837360710391 1.526147873045863E-4 88-89 9.91996117479811E-4 0.0 0.0 3.4841955941637055 1.526147873045863E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 75 6.9331454E-6 37.991913 5 ATACCGT 120 1.5089063E-7 31.659925 6 TAAGGTG 640 0.0 28.939152 5 GTAAGGT 600 0.0 27.702436 4 GGTAAGG 680 0.0 23.048323 3 GTACATG 4560 0.0 23.02472 1 ACCGCTA 105 3.4457116E-8 22.622002 60-61 TACATGG 4610 0.0 22.564577 2 ACATGGG 4485 0.0 22.025976 3 GAGTACT 4770 0.0 21.803535 12-13 ACCGTAT 135 3.801798E-4 21.106617 8 CTAGGAC 620 0.0 20.682657 3 AGGTAAG 740 0.0 20.540087 2 GTACTTT 5115 0.0 20.193647 14-15 CATGGGG 2400 0.0 19.985329 4 TAGCCCT 715 0.0 19.925827 4 TACCGTC 120 0.0047697187 19.787453 7 GTGTAGC 780 0.0 19.490469 1 AGTACTT 5110 0.0 19.37699 12-13 GGTGTGC 1225 0.0 18.995956 8 >>END_MODULE