FastQCFastQC Report
Thu 26 May 2016
SRR936639_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936639_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600193
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43680.7277659019682002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34720.578480588743954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20650.34405599532150494No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10010.16677968586771255No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6980.11629592481085252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34450.033.923131
GTATCAA49850.030.4001371
TACACCG650.005833413529.2394395
TAAGGTG2450.029.0902585
TACCTGG5150.026.7555072
GTACATG36950.025.1994821
TATCAAC60250.025.1568372
GTACTAG950.001224943325.0032461
ATCAACG60900.024.7322943
TCAACGC61450.024.4336094
CAACGCA61300.024.3383755
TACATGG36750.024.3065282
AACGCAG63650.023.5890876
ACATGGG37000.023.3717963
GTAAGGT2452.9467628E-1023.2722054
GAGTACT36550.023.00380512-13
GTACCTG6200.022.9868561
CTTAGAC1503.0231626E-522.1732433
ACGCAGA71450.020.9456837
AGTACTT37650.020.94386912-13
Produced by FastQC (version 0.11.5)