##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936639_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 600193 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.559839918159657 31.0 31.0 34.0 30.0 34.0 2 31.623356153770537 33.0 31.0 34.0 30.0 34.0 3 31.6185910198886 33.0 31.0 34.0 28.0 34.0 4 35.2609377316963 37.0 35.0 37.0 33.0 37.0 5 35.28112457159614 37.0 35.0 37.0 33.0 37.0 6 35.282362506727004 37.0 35.0 37.0 33.0 37.0 7 35.20148185666944 37.0 35.0 37.0 32.0 37.0 8 35.179538914982345 37.0 35.0 37.0 32.0 37.0 9 36.68651083901345 39.0 37.0 39.0 32.0 39.0 10-11 36.56629784086119 39.0 37.0 39.0 32.0 39.0 12-13 36.47627263230328 39.0 37.0 39.0 32.0 39.0 14-15 37.58370640777217 40.0 37.0 41.0 32.0 41.0 16-17 37.50378461594854 40.0 37.0 41.0 32.0 41.0 18-19 37.519595363491405 40.0 37.0 41.0 32.0 41.0 20-21 37.42958181784859 40.0 37.0 41.0 32.0 41.0 22-23 37.51679459773773 40.0 37.0 41.0 32.0 41.0 24-25 37.558201278588726 40.0 37.0 41.0 32.0 41.0 26-27 37.46459805429254 40.0 37.0 41.0 32.0 41.0 28-29 37.31390402753781 40.0 37.0 41.0 31.5 41.0 30-31 37.04979814826231 39.0 36.0 41.0 31.0 41.0 32-33 36.80978535237832 39.0 35.5 41.0 30.5 41.0 34-35 36.59323084407849 39.0 35.0 41.0 30.0 41.0 36-37 36.377879282164244 39.0 35.0 41.0 30.0 41.0 38-39 36.1744597154582 39.0 35.0 40.0 30.0 41.0 40-41 35.93569984988162 39.0 35.0 40.0 29.5 41.0 42-43 35.656635448930594 38.0 35.0 40.0 28.5 41.0 44-45 35.2936380464284 38.0 35.0 40.0 26.5 41.0 46-47 34.94483774385906 38.0 34.5 40.0 24.5 41.0 48-49 34.672179615557 38.0 34.0 40.0 23.5 41.0 50-51 33.68810949144692 37.5 33.0 39.5 22.0 40.5 52-53 33.776758476023545 38.0 33.5 39.5 22.0 40.5 54-55 34.20200002332583 38.0 34.0 40.0 22.0 41.0 56-57 34.103013530647644 38.0 34.0 40.0 21.5 41.0 58-59 33.88454797040285 38.0 34.0 40.0 20.0 41.0 60-61 33.61802037011428 38.0 33.0 40.0 19.5 41.0 62-63 33.253889998717085 37.0 33.0 40.0 18.0 41.0 64-65 32.89351758517677 36.5 32.5 39.5 15.0 41.0 66-67 32.44123057083304 36.0 32.0 39.0 12.0 41.0 68-69 32.0658513178261 35.5 31.5 39.0 8.0 40.5 70-71 31.679875473389394 35.0 31.0 38.5 7.5 40.0 72-73 31.29400126292709 35.0 31.0 37.5 4.5 40.0 74-75 30.864726846197804 35.0 31.0 37.0 2.0 39.0 76-77 30.402398895022102 35.0 30.0 36.5 2.0 39.0 78-79 29.92763827635444 34.5 30.0 36.0 2.0 39.0 80-81 29.4919850781332 34.0 29.0 36.0 2.0 37.5 82-83 29.087386890550206 34.0 29.0 35.0 2.0 37.0 84-85 28.738328337718034 34.0 29.0 35.0 2.0 36.5 86-87 28.384602952716875 34.0 29.0 35.0 2.0 36.0 88-89 27.99175431902738 34.0 27.5 35.0 2.0 36.0 90-91 27.603498374689472 34.0 27.0 35.0 2.0 35.5 92-93 27.15235932441731 33.5 26.0 35.0 2.0 35.0 94-95 26.690627014976847 33.0 25.0 35.0 2.0 35.0 96-97 26.14240669251391 33.0 24.5 35.0 2.0 35.0 98-99 25.426239559608327 33.0 21.0 35.0 2.0 35.0 100-101 22.646023862324284 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33748668446509456 1101 2 0.37163409688668736 1101 3 0.47005430383233104 1101 4 0.4313180167834645 1101 5 0.3014457619822437 1101 6 0.4281144212036381 1101 7 0.42660409533713306 1101 8 0.4549188600433638 1101 9 0.48777622065446025 1101 10-11 0.6333242191156785 1101 12-13 0.678688804362622 1101 14-15 0.8185144090428267 1101 16-17 0.7716830385581162 1101 18-19 0.5185007332643039 1101 20-21 0.5826707570479925 1101 22-23 0.6152498160658837 1101 24-25 0.5955647035289076 1101 26-27 0.6799455671011927 1101 28-29 0.6253915790910938 1101 30-31 0.6178552585818196 1101 32-33 0.5604092439861006 1101 34-35 0.5656669694323853 1101 36-37 0.4743963961080837 1101 38-39 0.6174911249342259 1101 40-41 0.6734934968969455 1101 42-43 0.9250155244407097 1101 44-45 0.9631958405383401 1101 46-47 1.1125612278058412 1101 48-49 1.2118580655984132 1101 50-51 1.0729517930990653 1101 52-53 1.0837445703650133 1101 54-55 1.2443436244907176 1101 56-57 1.1682530108926272 1101 58-59 1.3345440067288408 1101 60-61 1.4211748203057937 1101 62-63 1.2290387793599749 1101 64-65 1.0443174857974356 1101 66-67 1.1564233405454232 1101 68-69 1.1143526749504922 1101 70-71 1.2236952693591405 1101 72-73 1.1432798700459337 1101 74-75 0.9888022157339869 1101 76-77 0.9219806321820307 1101 78-79 1.0029637234505309 1101 80-81 0.9980921670484157 1101 82-83 0.7814816249029093 1101 84-85 1.023070795672819 1101 86-87 1.1574920348210433 1101 88-89 1.4214080077137012 1101 90-91 1.4157910695596918 1101 92-93 1.1398869109776513 1101 94-95 1.2296514960964444 1101 96-97 0.8678532577863223 1101 98-99 0.8375424194667289 1101 100-101 0.9763881881564132 1102 1 0.4349493261259134 1102 2 0.36685356668242974 1102 3 0.45739759938944147 1102 4 0.3670783885281921 1102 5 0.4305067053847793 1102 6 0.42432342732138295 1102 7 0.48577569297026457 1102 8 0.5374448520869564 1102 9 0.7190001032473177 1102 10-11 0.5893968296863221 1102 12-13 0.6806845921471947 1102 14-15 0.6746672522882378 1102 16-17 0.5179739488646717 1102 18-19 0.45105179801876005 1102 20-21 0.5085859109944337 1102 22-23 0.3741840587028662 1102 24-25 0.40618884687794576 1102 26-27 0.24040423055854632 1102 28-29 0.1702817606175131 1102 30-31 0.35601926754112867 1102 32-33 0.2802802031008653 1102 34-35 0.549924650039479 1102 36-37 0.27802358161270746 1102 38-39 0.23888984789748946 1102 40-41 0.12029589050507639 1102 42-43 0.46712430657556325 1102 44-45 0.3423821474951865 1102 46-47 0.39915234203710526 1102 48-49 0.42221810972638707 1102 50-51 0.6281485303036263 1102 52-53 0.7126951938397568 1102 54-55 0.5340571269812742 1102 56-57 0.5792616024748867 1102 58-59 0.6409659637375 1102 60-61 0.7078027078463265 1102 62-63 0.6779405946242392 1102 64-65 0.665823185660372 1102 66-67 0.6206997421099558 1102 68-69 0.6789867805233207 1102 70-71 0.7487245208551485 1102 72-73 0.5383422175202774 1102 74-75 0.7186186834904795 1102 76-77 0.5632001688383319 1102 78-79 0.6310551886997509 1102 80-81 0.6586043189556143 1102 82-83 0.6537980090836975 1102 84-85 0.542978528096846 1102 86-87 0.5661137043292861 1102 88-89 0.806611765277303 1102 90-91 0.5278025695764086 1102 92-93 0.8366608888468043 1102 94-95 0.6339974326457707 1102 96-97 0.7317251529489575 1102 98-99 0.8532780528034039 1102 100-101 0.8308528022038217 1103 1 0.2808558879911409 1103 2 0.27247928424844403 1103 3 0.39750181205393176 1103 4 0.5197234472582153 1103 5 0.4641550318000398 1103 6 0.5506503724772571 1103 7 0.43739628821887777 1103 8 0.39172375343819255 1103 9 0.16921647699985698 1103 10-11 0.45273693389898995 1103 12-13 0.3366856519370103 1103 14-15 0.5051185461149643 1103 16-17 0.6696916915123836 1103 18-19 0.6288933594424151 1103 20-21 0.6059364364735487 1103 22-23 0.1590555591886016 1103 24-25 0.3790229014571196 1103 26-27 0.3120990110937427 1103 28-29 0.49172484107322134 1103 30-31 0.3061296623128342 1103 32-33 0.20159964141226538 1103 34-35 0.6199944195351961 1103 36-37 0.6355878313698398 1103 38-39 0.5920648015316843 1103 40-41 0.39879982055894914 1103 42-43 0.6885673870312772 1103 44-45 0.8961399855239804 1103 46-47 1.2455799672247778 1103 48-49 0.936816714336473 1103 50-51 1.1096905244747788 1103 52-53 1.131577115591945 1103 54-55 1.0420850347795607 1103 56-57 0.9069221164729413 1103 58-59 0.981184986964557 1103 60-61 0.9910315341291067 1103 62-63 1.198456358997639 1103 64-65 1.2013614705671998 1103 66-67 1.1171406146702338 1103 68-69 1.125564542600511 1103 70-71 1.2906038814486251 1103 72-73 0.8786583881464374 1103 74-75 1.0093455226673775 1103 76-77 1.14464501561039 1103 78-79 1.0259063765474927 1103 80-81 1.1557517180547912 1103 82-83 0.9641309597151562 1103 84-85 1.147138203094638 1103 86-87 0.8494838359198553 1103 88-89 0.49531617223659197 1103 90-91 0.5026767195366659 1103 92-93 0.5241818638534319 1103 94-95 0.8987505899362418 1103 96-97 0.8568774977339118 1103 98-99 0.8337867519114184 1103 100-101 1.0866154092867504 1104 1 0.20485712217158536 1104 2 0.24998727265350595 1104 3 0.2867063086023336 1104 4 0.20245167463344416 1104 5 0.35210945717118847 1104 6 0.33181508392137005 1104 7 0.5059581899161429 1104 8 0.45864493671530937 1104 9 0.6123124159900257 1104 10-11 0.5049466068544177 1104 12-13 0.775498375648759 1104 14-15 0.5662965528076143 1104 16-17 0.5247708339953405 1104 18-19 0.4954932850207996 1104 20-21 0.37454415645760974 1104 22-23 0.6152322217898885 1104 24-25 0.6097398252226469 1104 26-27 0.37840509049180326 1104 28-29 0.3966366673557076 1104 30-31 0.6690800156830576 1104 32-33 0.5883860977385709 1104 34-35 0.6612136626859595 1104 36-37 0.5293609143181541 1104 38-39 0.2983583272488559 1104 40-41 0.1026706712562202 1104 42-43 0.25340291129973735 1104 44-45 0.34881936213043474 1104 46-47 0.34575185201165226 1104 48-49 0.5510326985622669 1104 50-51 0.2289074555235544 1104 52-53 0.5107439643399445 1104 54-55 0.6131020550812991 1104 56-57 0.6983118539712336 1104 58-59 0.7350249169393877 1104 60-61 0.7216537755967778 1104 62-63 1.0166915074502114 1104 64-65 0.9539513293647772 1104 66-67 1.0155537923273315 1104 68-69 1.0210736883807918 1104 70-71 0.6569621354275981 1104 72-73 0.6034268799964799 1104 74-75 1.0180556319559066 1104 76-77 0.7955950829473828 1104 78-79 0.8148515892648511 1104 80-81 0.9427112509731117 1104 82-83 0.9578349179648988 1104 84-85 0.867124907846101 1104 86-87 0.7849469250523136 1104 88-89 0.7618044653994716 1104 90-91 0.9572470936442983 1104 92-93 0.49628634014114326 1104 94-95 0.632941625707609 1104 96-97 0.9199147204571325 1104 98-99 0.6853030591164071 1104 100-101 0.9210365958744404 1105 1 0.35449747028944145 1105 2 0.14186522169408633 1105 3 0.4742453670064428 1105 4 0.21625895155491293 1105 5 0.2300333326037105 1105 6 0.3416840154512002 1105 7 0.3514525801856152 1105 8 0.38837545994064016 1105 9 0.5636262463349979 1105 10-11 0.6706842602045242 1105 12-13 0.42569445408013706 1105 14-15 0.309331050028959 1105 16-17 0.42795422499879265 1105 18-19 0.47972312669008943 1105 20-21 0.5479404026566215 1105 22-23 0.3551881827839125 1105 24-25 0.40522058437122155 1105 26-27 0.2598017561099226 1105 28-29 0.06041982767865761 1105 30-31 0.021337539013252638 1105 32-33 -0.05330416125273274 1105 34-35 -0.11154926066519977 1105 36-37 0.08451452656519365 1105 38-39 -0.06266295827069968 1105 40-41 0.013883841016095744 1105 42-43 0.109709926084264 1105 44-45 0.24940025759021722 1105 46-47 0.064990606336913 1105 48-49 -0.056770593877701 1105 50-51 -0.1049732197412041 1105 52-53 0.16034446764287935 1105 54-55 0.039751944983784426 1105 56-57 -0.06725803173705458 1105 58-59 0.16834986189062562 1105 60-61 0.030327362678676195 1105 62-63 0.25326065917507634 1105 64-65 0.13463078831412645 1105 66-67 0.12699344840366678 1105 68-69 0.34819961896693385 1105 70-71 0.2418462708999698 1105 72-73 0.16690596146863967 1105 74-75 0.4724899404386598 1105 76-77 0.42773307183277254 1105 78-79 0.4100618230909987 1105 80-81 0.713788109524021 1105 82-83 0.48531316059884944 1105 84-85 0.43395934215458354 1105 86-87 0.4728592664594622 1105 88-89 0.2592816594856586 1105 90-91 0.2666693438277967 1105 92-93 0.13644571940086792 1105 94-95 0.40057085921920077 1105 96-97 0.17628674520286225 1105 98-99 0.17535443795671313 1105 100-101 -0.3235707229889364 1106 1 0.28761979299291696 1106 2 0.2968374571375243 1106 3 0.2985528396385213 1106 4 0.28707126315256204 1106 5 0.2343265263209915 1106 6 0.35100021408676696 1106 7 0.577912995860693 1106 8 0.3970284823109438 1106 9 0.4671769364825451 1106 10-11 0.5073636192399107 1106 12-13 0.6265146984082364 1106 14-15 0.7408588939128293 1106 16-17 0.6251357258713952 1106 18-19 0.1991186526301192 1106 20-21 0.11492517181416417 1106 22-23 0.4601199613020839 1106 24-25 0.28474224072534327 1106 26-27 0.3813557780042558 1106 28-29 0.4306205257589397 1106 30-31 0.4704102028241337 1106 32-33 0.3414205730007964 1106 34-35 0.1530009535185215 1106 36-37 0.0954815264065374 1106 38-39 0.19862373060761485 1106 40-41 0.2326986910874922 1106 42-43 0.3547083395381705 1106 44-45 0.14699505371904564 1106 46-47 0.31146530092679114 1106 48-49 0.5240885208295794 1106 50-51 0.20986574583074713 1106 52-53 0.3607752149060772 1106 54-55 0.46589981107928935 1106 56-57 0.5230656236651328 1106 58-59 0.29580424869056543 1106 60-61 0.5200219525565046 1106 62-63 0.48211358635261803 1106 64-65 0.5578760683395174 1106 66-67 0.7080738862903857 1106 68-69 0.7359990795412621 1106 70-71 0.5859863629196411 1106 72-73 0.26872652310595413 1106 74-75 0.5527120568911421 1106 76-77 0.5021341506841139 1106 78-79 0.49192680801881394 1106 80-81 0.6706237433491822 1106 82-83 0.7319393703402426 1106 84-85 0.9414597387911066 1106 86-87 0.9779116224804092 1106 88-89 0.7608110899156131 1106 90-91 0.782139754743234 1106 92-93 0.7858507646174324 1106 94-95 0.7674247383243866 1106 96-97 0.8473552839939131 1106 98-99 0.9724700971666138 1106 100-101 0.872420075043749 1107 1 0.17239454112617736 1107 2 0.21277042261212387 1107 3 0.3302705741484644 1107 4 0.33780250000474865 1107 5 0.1505468114227142 1107 6 0.10803870483541544 1107 7 0.43379279880311117 1107 8 0.40131979416867125 1107 9 0.43337067392280915 1107 10-11 0.517066957524321 1107 12-13 0.3031356179654594 1107 14-15 0.029445415765920302 1107 16-17 0.18398332213187274 1107 18-19 0.181998771456108 1107 20-21 0.29439156381894804 1107 22-23 0.3055495629527414 1107 24-25 0.16365213997864458 1107 26-27 0.214807007597841 1107 28-29 0.18472449177793493 1107 30-31 0.18365597368084963 1107 32-33 -0.05782433085634153 1107 34-35 -0.019347136730083037 1107 36-37 0.006870305146904343 1107 38-39 0.010163898227524726 1107 40-41 0.11046687287512924 1107 42-43 -0.03879437235584504 1107 44-45 0.06094030504811343 1107 46-47 0.0960646328666499 1107 48-49 -0.03355434927506451 1107 50-51 0.025999667021160633 1107 52-53 0.02113569182305497 1107 54-55 0.19824729644447103 1107 56-57 -0.020417093471216674 1107 58-59 0.11317734078742347 1107 60-61 0.12625376404794508 1107 62-63 0.18488395244150269 1107 64-65 0.41175219493489834 1107 66-67 0.30360682219291135 1107 68-69 0.26529223965390614 1107 70-71 0.19310701700025135 1107 72-73 0.2594138387005209 1107 74-75 0.4428349826450635 1107 76-77 0.5228161231417552 1107 78-79 0.313912136235281 1107 80-81 0.3563422776494356 1107 82-83 0.2586760578641858 1107 84-85 0.26684921650787174 1107 86-87 0.4246692792964346 1107 88-89 0.49922253162165475 1107 90-91 0.21588317663598033 1107 92-93 0.23629400974980186 1107 94-95 0.572695961331462 1107 96-97 0.4448431695230859 1107 98-99 0.6593152268468998 1107 100-101 0.6375690443896005 1108 1 0.37289821785318367 1108 2 0.21700605736355527 1108 3 0.2964213114731358 1108 4 0.3375725278464614 1108 5 0.1435722459664177 1108 6 0.1663856574215643 1108 7 0.24804315554271028 1108 8 0.18905360705050356 1108 9 0.1373493807629771 1108 10-11 0.3600421563465659 1108 12-13 0.20915994600377275 1108 14-15 0.29214242367734045 1108 16-17 0.29641237477853366 1108 18-19 0.22397623041456427 1108 20-21 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2316 78-79 -3.325053865817793 2316 80-81 -3.1706006052095255 2316 82-83 -3.173459790587895 2316 84-85 -2.971145897433413 2316 86-87 -2.8700635578426734 2316 88-89 -2.9558817977467626 2316 90-91 -3.0114945612326594 2316 92-93 -3.7942006457282567 2316 94-95 -3.617903539944855 2316 96-97 -4.204223778755939 2316 98-99 -5.4246963158871395 2316 100-101 -5.919907489670528 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1285.0 3 476.0 4 473.0 5 523.0 6 593.0 7 737.0 8 931.0 9 1127.0 10 1528.0 11 2074.0 12 2708.0 13 3510.0 14 4005.0 15 4987.0 16 6395.0 17 8825.0 18 6908.0 19 4276.0 20 4070.0 21 4214.0 22 4484.0 23 5179.0 24 5718.0 25 6539.0 26 7580.0 27 8959.0 28 10427.0 29 12397.0 30 15023.0 31 18646.0 32 23429.0 33 30431.0 34 40832.0 35 56927.0 36 80824.0 37 109512.0 38 89754.0 39 13885.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.265280277277874 18.413817236552692 14.825868159701393 21.49503432646804 2 15.415968192922058 20.276512285917104 40.99227151993441 23.315248001226426 3 19.1244865481874 26.02477899700886 30.526991109889103 24.32374334491464 4 12.35751519837884 16.961190806313994 37.925287969283275 32.75600602602389 5 13.005615121660968 37.3789765444918 34.24824204524432 15.36716628860292 6 30.10498346402091 36.16978716594655 18.521130047474262 15.204099322558276 7 27.448742408211213 33.90657413501512 21.062058968099908 17.58262448867376 8 26.584162293412707 33.485765329164785 20.24951295644189 19.68055942098062 9 27.097202174789903 16.72394769036912 21.02469139917929 35.15415873566169 10-11 24.659585852373024 26.752409860467285 27.26096834469297 21.32703594246672 12-13 26.338447538356537 24.64110147492773 27.94562625886361 21.074824727852125 14-15 23.19214484340769 25.285181083425623 27.50238119642308 24.020292876743607 16-17 21.203497611287823 28.830043524310867 27.975890381735624 21.99056848266569 18-19 20.986264523741276 26.522368061434676 31.686087205775497 20.805280209048547 20-21 22.12641498582618 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21.45168935833791 46-47 21.647163104319553 26.572940712891512 30.21537364449062 21.56452253829832 48-49 21.751206280656856 26.746774365536364 29.793632704201322 21.70838664960546 50-51 21.649685508393386 27.026950472778772 29.679760069979586 21.643603948848252 52-53 21.900177531405397 27.1534742754411 29.327005184316924 21.61934300883658 54-55 21.452791079741804 27.04011583539111 29.510115602120763 21.996977482746324 56-57 21.593650656127746 27.398831699400855 29.157114104247334 21.850403540224065 58-59 21.892174921459294 26.972348506622744 29.226389676864674 21.90908689505329 60-61 21.58080513977855 26.946432037641948 29.825477792967625 21.647285029611876 62-63 21.57651218276818 27.012772579441247 29.720322830027722 21.69039240776285 64-65 21.34474643340623 27.114526710403 29.643236488597314 21.897490367593463 66-67 21.427178803532744 27.289951674720882 29.223962672887854 22.058906848858523 68-69 21.307844047439474 27.232194571512146 29.502995593905972 21.956965787142405 70-71 21.36602335072678 27.156116835308953 29.316754967384156 22.161104846580105 72-73 21.494718196913485 27.22417690270301 29.179264705024693 22.10184019535881 74-75 21.403657004202955 27.514440367033632 29.082715888903333 21.99918673986008 76-77 21.483780653440103 27.306746881535858 29.037254918299155 22.172217546724884 78-79 21.459583402443975 27.383983723506315 28.952454934999878 22.20397793904983 80-81 21.473376184878575 27.603481479628844 28.79890620180243 22.12423613369015 82-83 21.464779059630313 27.67759658483813 28.850433312830358 22.00719104270119 84-85 21.366391212116667 27.502045495826522 28.647523158678815 22.48404013337799 86-87 21.437653520685508 27.49299262434134 28.660499731703332 22.40885412326982 88-89 21.509461003202134 27.19547851620273 28.698140295649583 22.596920184945553 90-91 21.29521450797582 27.151038081603197 29.10486815855307 22.448879251867915 92-93 21.436386103757698 27.15105035067239 28.99769440299036 22.41486914257955 94-95 21.147974082519696 27.103129209986744 29.01561397384424 22.733282733649318 96-97 21.156190347638653 26.94080819341717 29.113726823326108 22.789274635618067 98-99 21.032026838365528 27.27253026609156 28.742722593819675 22.95272030172324 100-101 21.025222063365693 27.338865986686617 28.729309863323177 22.90660208662451 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 173.0 1 140.0 2 232.5 3 333.5 4 256.5 5 213.5 6 339.5 7 708.0 8 1155.0 9 1403.0 10 1286.5 11 1066.5 12 1071.0 13 1174.5 14 1256.5 15 1290.0 16 1312.5 17 1396.5 18 1514.5 19 1609.5 20 1746.0 21 1989.0 22 2224.0 23 2530.5 24 2934.0 25 3409.5 26 4216.5 27 5104.5 28 5858.0 29 6791.5 30 8204.0 31 9768.5 32 11325.5 33 12514.0 34 13451.0 35 14555.5 36 15631.0 37 16614.5 38 17134.0 39 17185.0 40 17284.0 41 17949.0 42 18675.0 43 19314.0 44 20201.5 45 20931.0 46 21078.0 47 21609.5 48 23666.5 49 23421.5 50 21174.5 51 19800.0 52 18544.0 53 18159.0 54 17641.0 55 16343.5 56 14918.0 57 13290.0 58 12135.0 59 10858.0 60 9057.5 61 7575.0 62 6264.0 63 4850.5 64 3531.5 65 2694.0 66 2054.5 67 1603.5 68 1210.5 69 816.5 70 613.0 71 457.5 72 347.5 73 287.5 74 233.5 75 187.0 76 130.5 77 84.5 78 48.5 79 31.0 80 28.0 81 18.5 82 12.0 83 8.5 84 5.0 85 2.5 86 3.0 87 3.0 88 1.5 89 1.0 90 2.0 91 1.5 92 0.0 93 0.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012162754314028986 2 0.01249598045961882 3 0.014661950405952752 4 0.021493086390544373 5 0.03432229299575303 6 0.048151178037731196 7 0.00633129676620687 8 0.02399228248246814 9 0.0378211675244463 10-11 0.04365262507226842 12-13 0.03290608187699623 14-15 0.029907046566687714 16-17 0.030573498857867382 18-19 0.02349244326408339 20-21 0.05323287675797619 22-23 0.012745900068811199 24-25 0.0029157287739110587 26-27 7.497588275771293E-4 28-29 0.0 30-31 0.003165648383103435 32-33 0.0021659699463339294 34-35 0.0 36-37 3.3322614558983527E-4 38-39 0.0 40-41 0.0019993568735390114 42-43 0.003582181065090729 44-45 0.0015828241915517174 46-47 5.831457547822116E-4 48-49 1.6661307279491763E-4 50-51 0.0029990353103085173 52-53 0.0029157287739110587 54-55 0.005165005256642447 56-57 8.330653639745881E-4 58-59 0.004415246429065317 60-61 0.0019160503371415526 62-63 1.6661307279491763E-4 64-65 9.16371900372047E-4 66-67 0.01549501576992734 68-69 0.03807108713363868 70-71 0.042403027026306536 72-73 0.011079769340862022 74-75 0.02315921711849355 76-77 0.022992604045698636 78-79 0.051566746030027005 80-81 0.07464265661212309 82-83 0.04665166038257694 84-85 0.015661628842722257 86-87 0.018494051080235857 88-89 0.020743327562967248 90-91 0.03198970997662418 92-93 0.02190961907253167 94-95 0.009580251685707763 96-97 0.01691122688868414 98-99 0.050900293738847344 100-101 0.07006079711026286 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 600193.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.1398125274087 #Duplication Level Percentage of deduplicated Percentage of total 1 86.04846057208555 58.63325971654027 2 8.580353000873837 11.693272897970637 3 2.1605944521152587 4.41667502744679 4 0.9717487629203433 2.6485911411653404 5 0.524507652565307 1.786992655749561 6 0.32702803378583406 1.3370177348024286 7 0.2366903403120164 1.1289624791136554 8 0.16912599982255486 0.9219371137135555 9 0.1276009963043868 0.7825237169842335 >10 0.7835839319706637 9.67410769367095 >50 0.045074412611672145 2.1426083665470395 >100 0.0232720897096777 2.636026954349835 >500 9.798774614533333E-4 0.3775768091890538 >1k 9.798774614533333E-4 1.8204476927567728 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4368 0.7277659019682002 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3472 0.578480588743954 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2065 0.34405599532150494 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1001 0.16677968586771255 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 698 0.11629592481085252 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.6661307279491763E-4 0.0 0.0 0.0 0.0 8 1.6661307279491763E-4 0.0 0.0 0.0 0.0 9 1.6661307279491763E-4 0.0 0.0 0.0 0.0 10-11 1.6661307279491763E-4 0.0 0.0 0.0 0.0 12-13 1.6661307279491763E-4 0.0 0.0 0.0 0.0 14-15 1.6661307279491763E-4 0.0 0.0 0.0 0.0 16-17 1.6661307279491763E-4 0.0 0.0 0.0 0.0 18-19 1.6661307279491763E-4 0.0 0.0 8.330653639745882E-5 0.0 20-21 1.6661307279491763E-4 0.0 0.0 4.1653268198729406E-4 8.330653639745882E-5 22-23 1.6661307279491763E-4 0.0 0.0 4.998392183847529E-4 1.6661307279491763E-4 24-25 1.6661307279491763E-4 0.0 0.0 7.497588275771293E-4 1.6661307279491763E-4 26-27 1.6661307279491763E-4 0.0 0.0 0.0014162111187567999 1.6661307279491763E-4 28-29 1.6661307279491763E-4 0.0 0.0 0.0019160503371415528 1.6661307279491763E-4 30-31 1.6661307279491763E-4 0.0 0.0 0.004165326819872941 1.6661307279491763E-4 32-33 1.6661307279491763E-4 0.0 0.0 0.007164362130181458 1.6661307279491763E-4 34-35 1.6661307279491763E-4 0.0 0.0 0.01057993012247727 1.6661307279491763E-4 36-37 1.6661307279491763E-4 0.0 0.0 0.014495337333157835 1.6661307279491763E-4 38-39 1.6661307279491763E-4 0.0 0.0 0.02190961907253167 1.6661307279491763E-4 40-41 1.6661307279491763E-4 0.0 0.0 0.027574463547558867 1.6661307279491763E-4 42-43 1.6661307279491763E-4 0.0 0.0 0.041569961662331956 1.6661307279491763E-4 44-45 1.6661307279491763E-4 0.0 0.0 0.06039723888815764 1.6661307279491763E-4 46-47 1.6661307279491763E-4 0.0 0.0 0.0742261239301358 1.6661307279491763E-4 48-49 1.6661307279491763E-4 0.0 0.0 0.09155388350080723 1.6661307279491763E-4 50-51 1.6661307279491763E-4 0.0 0.0 0.11279705028215925 1.6661307279491763E-4 52-53 1.6661307279491763E-4 0.0 0.0 0.15653298189082512 1.6661307279491763E-4 54-55 1.6661307279491763E-4 0.0 0.0 0.1893557572314239 1.6661307279491763E-4 56-57 1.6661307279491763E-4 0.0 0.0 0.2429218601349899 1.6661307279491763E-4 58-59 1.6661307279491763E-4 8.330653639745882E-5 0.0 0.3193972605478571 1.6661307279491763E-4 60-61 1.6661307279491763E-4 1.6661307279491763E-4 0.0 0.3897079772673123 1.6661307279491763E-4 62-63 1.6661307279491763E-4 1.6661307279491763E-4 0.0 0.48959251440786544 1.6661307279491763E-4 64-65 1.6661307279491763E-4 1.6661307279491763E-4 0.0 0.5623191206828471 1.6661307279491763E-4 66-67 1.6661307279491763E-4 1.6661307279491763E-4 0.0 0.6915275586353056 1.6661307279491763E-4 68-69 1.6661307279491763E-4 1.6661307279491763E-4 0.0 0.853558771928363 1.6661307279491763E-4 70-71 1.6661307279491763E-4 1.6661307279491763E-4 0.0 0.9594413796895331 1.6661307279491763E-4 72-73 1.6661307279491763E-4 1.6661307279491763E-4 0.0 1.1596269866526265 1.6661307279491763E-4 74-75 1.6661307279491763E-4 1.6661307279491763E-4 0.0 1.344900723600575 1.6661307279491763E-4 76-77 1.6661307279491763E-4 1.6661307279491763E-4 0.0 1.4616131810934148 1.6661307279491763E-4 78-79 1.6661307279491763E-4 1.6661307279491763E-4 0.0 1.5840737895976793 1.6661307279491763E-4 80-81 6.664522911796705E-4 1.6661307279491763E-4 0.0 1.8162491065373971 1.6661307279491763E-4 82-83 6.664522911796705E-4 1.6661307279491763E-4 0.0 2.0073543010331676 1.6661307279491763E-4 84-85 6.664522911796705E-4 1.6661307279491763E-4 0.0 2.2969278215507343 1.6661307279491763E-4 86-87 6.664522911796705E-4 1.6661307279491763E-4 0.0 2.7228741421509417 1.6661307279491763E-4 88-89 6.664522911796705E-4 1.6661307279491763E-4 0.0 3.2991887609485615 1.6661307279491763E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3445 0.0 33.92313 1 GTATCAA 4985 0.0 30.400137 1 TACACCG 65 0.0058334135 29.239439 5 TAAGGTG 245 0.0 29.090258 5 TACCTGG 515 0.0 26.755507 2 GTACATG 3695 0.0 25.199482 1 TATCAAC 6025 0.0 25.156837 2 GTACTAG 95 0.0012249433 25.003246 1 ATCAACG 6090 0.0 24.732294 3 TCAACGC 6145 0.0 24.433609 4 CAACGCA 6130 0.0 24.338375 5 TACATGG 3675 0.0 24.306528 2 AACGCAG 6365 0.0 23.589087 6 ACATGGG 3700 0.0 23.371796 3 GTAAGGT 245 2.9467628E-10 23.272205 4 GAGTACT 3655 0.0 23.003805 12-13 GTACCTG 620 0.0 22.986856 1 CTTAGAC 150 3.0231626E-5 22.173243 3 ACGCAGA 7145 0.0 20.945683 7 AGTACTT 3765 0.0 20.943869 12-13 >>END_MODULE