FastQCFastQC Report
Thu 26 May 2016
SRR936639_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936639_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600193
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42770.7126041123438627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37910.6316301589655328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21140.3522200358884559No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11730.1954371343884384No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10810.18010873169130595No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.10046768289533534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38400.029.821921
GGTAAGG3050.029.593413
GTATCAA54850.029.0213741
GTACCTG6050.028.274631
TACCTGG5750.028.0947172
GTAAGGT3200.026.7172264
AGGTAAG3500.024.4353222
GGTGTGC5850.024.3575868
TAAGGTG3750.024.0652945
GCACCGT1401.778428E-523.7486466
ATCAACG67150.023.6288013
TCAACGC67650.023.4502534
GAGTACT38200.023.37563112-13
TATCAAC68050.023.3201832
CAACGCA68900.023.093755
AACGCAG69900.022.8313186
GTACATG36950.021.604521
AGTACTT39550.021.55692512-13
GTACTTT41850.021.22340214-15
CCTACCG804.9901137E-520.78093190-91
Produced by FastQC (version 0.11.5)