##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936639_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 600193 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.97960822602063 31.0 31.0 34.0 30.0 34.0 2 32.09199707427444 33.0 31.0 34.0 30.0 34.0 3 32.18909917309932 34.0 31.0 34.0 30.0 34.0 4 35.92148025718394 37.0 35.0 37.0 35.0 37.0 5 35.80013262400595 37.0 35.0 37.0 35.0 37.0 6 35.86108301829578 37.0 35.0 37.0 35.0 37.0 7 35.79865309991953 37.0 35.0 37.0 33.0 37.0 8 35.77397603770787 37.0 35.0 37.0 33.0 37.0 9 37.37803673151803 39.0 37.0 39.0 34.0 39.0 10-11 37.29082561775962 39.0 37.0 39.0 34.0 39.0 12-13 37.168885675107845 39.0 37.0 39.0 33.0 39.0 14-15 38.255029632135 40.0 38.0 41.0 33.0 41.0 16-17 38.24254448152511 40.0 37.5 41.0 33.5 41.0 18-19 38.41580791512064 40.0 38.0 41.0 33.5 41.0 20-21 38.44005261640839 40.0 38.0 41.0 34.0 41.0 22-23 38.34187669632935 40.0 38.0 41.0 34.0 41.0 24-25 38.225515792420104 40.0 38.0 41.0 33.0 41.0 26-27 38.014419528385034 40.0 37.0 41.0 33.0 41.0 28-29 38.000538160225126 40.0 37.0 41.0 33.0 41.0 30-31 37.8310926651927 40.0 37.0 41.0 33.0 41.0 32-33 37.58629640798876 40.0 36.5 41.0 33.0 41.0 34-35 37.37337156547977 40.0 36.0 41.0 32.5 41.0 36-37 37.173723285676445 39.0 36.0 41.0 32.0 41.0 38-39 36.95851417793943 39.0 35.0 41.0 31.0 41.0 40-41 36.70682430484861 39.0 35.0 41.0 31.0 41.0 42-43 36.399886369884356 39.0 35.0 40.5 30.0 41.0 44-45 36.0359284430175 39.0 35.0 40.0 30.0 41.0 46-47 35.52190378761498 38.5 35.0 40.0 28.0 41.0 48-49 35.01465361975231 38.0 34.5 40.0 26.0 41.0 50-51 34.6685349545896 38.0 34.0 40.0 24.0 41.0 52-53 34.38959301424708 38.0 34.0 40.0 24.0 41.0 54-55 34.05100026158252 38.0 33.5 40.0 23.0 41.0 56-57 33.71791740323529 37.5 33.0 40.0 22.0 41.0 58-59 33.35386034159012 37.0 33.0 40.0 20.5 41.0 60-61 32.98571876046538 36.5 32.0 39.5 19.5 40.5 62-63 32.67267612251392 36.0 32.0 39.0 18.0 40.0 64-65 32.369536132544035 36.0 31.5 39.0 17.5 40.0 66-67 31.929316903062848 35.0 31.0 38.5 13.5 40.0 68-69 31.487476528383368 35.0 31.0 38.0 10.0 40.0 70-71 30.98721411279372 35.0 30.0 37.5 7.5 39.5 72-73 30.438073253103582 34.0 29.5 37.0 7.0 39.0 74-75 29.908375305943256 34.0 29.0 36.0 4.0 39.0 76-77 28.532566191208495 32.5 27.0 35.0 2.0 37.0 78-79 29.29785918862766 34.0 29.0 35.0 2.0 37.0 80-81 29.145881408147048 34.0 29.0 35.0 2.0 37.0 82-83 28.746184977165676 34.0 29.0 35.0 2.0 36.5 84-85 28.30431711132919 33.5 28.0 35.0 2.0 36.0 86-87 27.890162830956044 33.0 27.0 35.0 2.0 36.0 88-89 27.405217988213792 33.0 26.0 35.0 2.0 35.0 90-91 26.853722052739702 32.5 25.5 34.5 2.0 35.0 92-93 26.296243041821548 32.0 25.0 34.0 2.0 35.0 94-95 25.593531580674885 32.0 23.5 34.0 2.0 35.0 96-97 24.82070434010393 31.5 19.0 34.0 2.0 35.0 98-99 23.731824596421486 31.0 2.0 34.0 2.0 35.0 100-101 20.063285476505058 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21303189855060722 1101 2 0.38017994120990295 1101 3 0.20931235346414923 1101 4 0.15453309658880698 1101 5 0.14954586650822677 1101 6 0.20112021244372613 1101 7 0.1852495895572659 1101 8 0.1412664747555894 1101 9 0.30270926235760953 1101 10-11 0.2971381257391812 1101 12-13 0.38415633138025385 1101 14-15 0.4481295350270429 1101 16-17 0.38325022155007105 1101 18-19 0.3860359090091734 1101 20-21 0.3096798542859247 1101 22-23 0.3782140872621724 1101 24-25 0.1707624901590563 1101 26-27 0.03128157124264419 1101 28-29 0.07164818720214328 1101 30-31 0.26173365342057764 1101 32-33 0.1623930327411358 1101 34-35 0.03655282802486681 1101 36-37 0.34705565461391785 1101 38-39 0.19926821105824644 1101 40-41 0.30689919238479746 1101 42-43 0.5879477950813197 1101 44-45 0.476913014839603 1101 46-47 0.329678924865469 1101 48-49 0.4591957187358915 1101 50-51 0.4763662573582934 1101 52-53 0.5648601039854242 1101 54-55 0.5784162321389488 1101 56-57 0.41313654836454106 1101 58-59 0.31166848099007893 1101 60-61 0.04711289898634874 1101 62-63 0.2160203157451619 1101 64-65 0.5126506055249891 1101 66-67 0.6919276150905915 1101 68-69 0.46580179272220334 1101 70-71 0.47773227526060325 1101 72-73 0.43048805895278974 1101 74-75 0.34121145786140517 1101 76-77 0.2812220080807748 1101 78-79 0.42610778890331247 1101 80-81 0.4501702802954064 1101 82-83 0.4091000330072987 1101 84-85 0.36086677028178116 1101 86-87 0.4879546540109807 1101 88-89 0.5338448676802123 1101 90-91 0.3847887122821838 1101 92-93 0.7406964517734309 1101 94-95 0.6889836656113602 1101 96-97 0.5650909785703178 1101 98-99 0.2650012858568722 1101 100-101 0.44041880875893114 1102 1 0.2757136757043561 1102 2 0.43826839773382176 1102 3 0.4134643943828138 1102 4 0.30615947976668423 1102 5 0.2623730653849634 1102 6 0.1898675798090821 1102 7 0.29424567821437364 1102 8 0.28583906861110364 1102 9 0.31781306338057647 1102 10-11 0.4055424984199547 1102 12-13 0.4944562010021585 1102 14-15 0.5509142774467222 1102 16-17 0.5648267936090434 1102 18-19 0.5016979957273193 1102 20-21 0.3644587129761305 1102 22-23 0.24688322776965066 1102 24-25 0.31307854003703994 1102 26-27 0.24090949221345426 1102 28-29 0.3074185880312186 1102 30-31 0.31831106123657094 1102 32-33 0.4678254534277926 1102 34-35 0.4461306035739625 1102 36-37 0.6601870690491154 1102 38-39 0.6609905391430217 1102 40-41 0.7463068078028385 1102 42-43 0.7906021594045214 1102 44-45 0.7232707857084577 1102 46-47 0.5630390024237144 1102 48-49 0.69954606808232 1102 50-51 0.6686839787503303 1102 52-53 0.8212937615924858 1102 54-55 0.8791851286053713 1102 56-57 0.7005032451184618 1102 58-59 0.7018433213604851 1102 60-61 0.8433753935539272 1102 62-63 0.9233515764010676 1102 64-65 0.8013846007778866 1102 66-67 0.905547111630554 1102 68-69 0.8941005257145491 1102 70-71 0.48714624243207183 1102 72-73 0.4830438039466074 1102 74-75 0.5585149713839535 1102 76-77 0.7619494509985714 1102 78-79 0.8305072139024787 1102 80-81 1.0269562930657727 1102 82-83 0.80857183456515 1102 84-85 0.46164903886066 1102 86-87 1.067328170542254 1102 88-89 0.7836894168728712 1102 90-91 0.6594585882558377 1102 92-93 0.6884783526192173 1102 94-95 0.5259642426180129 1102 96-97 0.49936035977581383 1102 98-99 0.4567105761320036 1102 100-101 0.249846148042856 1103 1 0.2372433056610781 1103 2 0.2722346610915878 1103 3 0.2817325212043329 1103 4 0.1921260963877245 1103 5 0.24221939586275454 1103 6 0.267161583165084 1103 7 0.29470492046466035 1103 8 0.19672840889194987 1103 9 0.26713744737775613 1103 10-11 0.3881089560725215 1103 12-13 0.17700499549125226 1103 14-15 0.3465779708142023 1103 16-17 0.3015926683772179 1103 18-19 0.16646881618126486 1103 20-21 0.24797014987448307 1103 22-23 0.2710199406549165 1103 24-25 0.39598059779558525 1103 26-27 0.3447688934322173 1103 28-29 0.489649445577804 1103 30-31 0.4547678306439735 1103 32-33 0.5559999654828971 1103 34-35 0.32180842747222016 1103 36-37 0.38745016186903314 1103 38-39 0.2550998741249231 1103 40-41 0.30145120416423055 1103 42-43 0.29407297102687835 1103 44-45 0.2733370225714751 1103 46-47 0.330529950052437 1103 48-49 0.590405380985267 1103 50-51 0.6691078946736724 1103 52-53 0.7994355967951705 1103 54-55 0.8787347179368155 1103 56-57 0.886143093215253 1103 58-59 0.7597961607140178 1103 60-61 0.6084729825321133 1103 62-63 0.8885728075235662 1103 64-65 0.6096862876099962 1103 66-67 0.426575107744668 1103 68-69 0.5563808401188624 1103 70-71 0.5306417824832899 1103 72-73 0.48579042586659327 1103 74-75 0.4695897748812605 1103 76-77 0.2887709647316825 1103 78-79 0.23035707701296104 1103 80-81 0.6575577237820127 1103 82-83 0.6133408887027443 1103 84-85 0.40366970476192066 1103 86-87 0.4225697143027425 1103 88-89 0.29135522981996687 1103 90-91 0.558707903808255 1103 92-93 0.6744008113315729 1103 94-95 0.7204268408693615 1103 96-97 0.5427145945192571 1103 98-99 0.12429867115982418 1103 100-101 0.10492872333209036 1104 1 0.25419077486284536 1104 2 0.17797479195179733 1104 3 0.15109875895356595 1104 4 -0.08542545911662813 1104 5 0.04499285886448945 1104 6 0.07963369161405609 1104 7 0.14504862382700878 1104 8 0.34867561986926887 1104 9 0.2722516156897754 1104 10-11 0.11829817500315443 1104 12-13 0.08207043221501209 1104 14-15 0.3173727856672812 1104 16-17 0.1719116881307201 1104 18-19 0.08537905603867557 1104 20-21 0.19339341947272004 1104 22-23 0.16528033948589638 1104 24-25 0.13170906220761935 1104 26-27 0.08037155618976755 1104 28-29 0.263230303598057 1104 30-31 0.32553245309996726 1104 32-33 0.40015621378623223 1104 34-35 0.540032584832872 1104 36-37 0.44577909214446976 1104 38-39 0.4773476980873568 1104 40-41 0.5620142394006109 1104 42-43 0.46829303387474397 1104 44-45 0.7144591998140939 1104 46-47 0.5151577824104763 1104 48-49 0.6919445311222532 1104 50-51 0.8594444736896918 1104 52-53 0.8109199636222044 1104 54-55 0.7171040901335957 1104 56-57 0.9555485281115779 1104 58-59 0.9077918755123946 1104 60-61 0.7419558212000936 1104 62-63 0.691986534735257 1104 64-65 0.9344108150923702 1104 66-67 0.8731603873620131 1104 68-69 0.8636513812116391 1104 70-71 0.680361642062163 1104 72-73 0.7653892377692841 1104 74-75 0.8746366724138248 1104 76-77 0.9572273254619645 1104 78-79 0.9907823925433732 1104 80-81 0.7473708354925606 1104 82-83 0.7304946726178798 1104 84-85 0.805673526483762 1104 86-87 0.9293696247849326 1104 88-89 0.6587622723290103 1104 90-91 0.7990830840687835 1104 92-93 0.5608164838341132 1104 94-95 0.6598162658498623 1104 96-97 1.0698844413193278 1104 98-99 1.3712843973068338 1104 100-101 1.0218314336294192 1105 1 0.1608490531474196 1105 2 0.2621522062330186 1105 3 0.29629745875434566 1105 4 0.11354696407725129 1105 5 0.14652583569158395 1105 6 0.05474116775959459 1105 7 0.14543448940318626 1105 8 0.13368558107300288 1105 9 0.27924752635350103 1105 10-11 0.24398969225176614 1105 12-13 0.1863443128902702 1105 14-15 0.3514988211081871 1105 16-17 0.2546421065012794 1105 18-19 0.3013106907246339 1105 20-21 0.012021919000879677 1105 22-23 -0.011739687776348262 1105 24-25 0.19153290618370988 1105 26-27 0.1315281043705383 1105 28-29 0.3587257424152952 1105 30-31 0.3916874284590932 1105 32-33 0.40437556997790836 1105 34-35 0.3412677740957477 1105 36-37 0.3120787670946541 1105 38-39 0.07985372723698703 1105 40-41 0.17047392273918405 1105 42-43 0.450813742693029 1105 44-45 0.3369232870532599 1105 46-47 0.4236594076595068 1105 48-49 0.4466430736408711 1105 50-51 0.709411968709091 1105 52-53 0.645805147642335 1105 54-55 0.604446021558573 1105 56-57 0.7455453824682934 1105 58-59 0.7035431599834041 1105 60-61 0.37531012548866016 1105 62-63 0.623344404086204 1105 64-65 0.4407964709589933 1105 66-67 0.31709453856259984 1105 68-69 0.3835213612892332 1105 70-71 0.3200743399755517 1105 72-73 0.2551721883826872 1105 74-75 0.6041595831824118 1105 76-77 0.4282790188666006 1105 78-79 0.5110487236747545 1105 80-81 0.4338990938547269 1105 82-83 0.37798549782496593 1105 84-85 0.3523716495832723 1105 86-87 0.34171942031811753 1105 88-89 0.43648482659135723 1105 90-91 0.5431451580962552 1105 92-93 0.48350076610316606 1105 94-95 0.63792049149734 1105 96-97 0.6822866642405785 1105 98-99 0.3286273772795738 1105 100-101 0.535508690628479 1106 1 -0.01858137680656924 1106 2 0.19259009295362262 1106 3 0.22356383984271133 1106 4 0.20482889331729126 1106 5 0.25285139494908293 1106 6 0.08137134386620914 1106 7 0.13977527950314084 1106 8 0.08939556044789754 1106 9 0.2214686908783321 1106 10-11 0.012920360187671065 1106 12-13 0.1761920044657117 1106 14-15 0.1480060398928984 1106 16-17 0.22539016298232184 1106 18-19 0.18900940562230062 1106 20-21 0.11216232832976658 1106 22-23 0.08667535268057946 1106 24-25 0.18927049146056163 1106 26-27 0.08221111246191981 1106 28-29 0.3349775272796407 1106 30-31 0.2027924578102045 1106 32-33 0.2690336692956947 1106 34-35 0.31164378551888205 1106 36-37 0.3258063571311496 1106 38-39 0.5789573286966103 1106 40-41 0.3604080810764927 1106 42-43 0.5207486943033715 1106 44-45 0.5004192413607385 1106 46-47 0.6062954539866539 1106 48-49 0.7427893531902896 1106 50-51 0.6472931364070149 1106 52-53 0.6995969927954349 1106 54-55 0.8270947602544325 1106 56-57 0.5938239799615417 1106 58-59 0.5482171247620755 1106 60-61 0.5464865494137783 1106 62-63 0.6216840836038955 1106 64-65 0.6870323903624467 1106 66-67 0.41586496534350204 1106 68-69 0.259810429986679 1106 70-71 0.2234957266168358 1106 72-73 0.4652086789427372 1106 74-75 0.5114077189907569 1106 76-77 0.521273624609897 1106 78-79 0.3878819776807845 1106 80-81 0.4235500537151111 1106 82-83 0.4251011570348666 1106 84-85 0.558608362259772 1106 86-87 0.6463330963454084 1106 88-89 0.45823478692770436 1106 90-91 0.5791153310297794 1106 92-93 0.5457671471915368 1106 94-95 0.4657532695395403 1106 96-97 0.49920003882411024 1106 98-99 0.5174985497371338 1106 100-101 0.12215631874207489 1107 1 0.11394354103009974 1107 2 0.19953113026149083 1107 3 0.13158123360165774 1107 4 0.08088659919608432 1107 5 0.0575026124449991 1107 6 0.0893776480956916 1107 7 0.14926690858862202 1107 8 0.02853756691343534 1107 9 -0.04610821332829573 1107 10-11 -0.04359423430520337 1107 12-13 0.17753114916055068 1107 14-15 0.310145882219949 1107 16-17 0.2335972549044385 1107 18-19 0.07659602156836343 1107 20-21 0.047309583839243885 1107 22-23 0.14001430918946056 1107 24-25 0.18437765664078398 1107 26-27 -0.009977322166982106 1107 28-29 0.23801387405259078 1107 30-31 0.29419958468113805 1107 32-33 0.418412991691838 1107 34-35 0.4747069151857204 1107 36-37 0.41394207280905704 1107 38-39 0.5540008466986066 1107 40-41 0.6254402743165812 1107 42-43 0.7011618968922733 1107 44-45 0.7199574800746191 1107 46-47 0.8153356048042539 1107 48-49 0.8308318160067643 1107 50-51 0.5997904648385486 1107 52-53 0.11138650680521067 1107 54-55 0.40431064986992027 1107 56-57 0.43429941833400676 1107 58-59 0.29276477277369395 1107 60-61 0.4445208463040018 1107 62-63 0.17018145236099258 1107 64-65 0.5042072151142776 1107 66-67 0.5297292295028129 1107 68-69 0.5965372713526378 1107 70-71 0.37475438821143925 1107 72-73 0.5005271500662722 1107 74-75 0.4388326294344047 1107 76-77 0.028422170537353963 1107 78-79 0.4121814890693969 1107 80-81 0.44441685166388467 1107 82-83 0.4344751698987217 1107 84-85 0.5145274826248531 1107 86-87 0.8655697334624008 1107 88-89 0.6160841575128977 1107 90-91 0.6765788531987553 1107 92-93 0.8246125255461223 1107 94-95 0.9718575514421026 1107 96-97 1.424991764018852 1107 98-99 1.5840449678191142 1107 100-101 1.2852328902628152 1108 1 -0.028631657038133085 1108 2 0.08962517379409007 1108 3 -0.11522036263131952 1108 4 -0.12177542278698894 1108 5 -0.030588034285720767 1108 6 -0.12104310670309104 1108 7 -0.0492311093152793 1108 8 -0.11668419599281066 1108 9 -0.0550149219177527 1108 10-11 -0.062305862413829516 1108 12-13 -0.29346879617393284 1108 14-15 -0.2789617315550075 1108 16-17 -0.07188305702828757 1108 18-19 -0.14076913840365535 1108 20-21 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13 2942.0 14 3458.0 15 5031.0 16 6784.0 17 9003.0 18 7975.0 19 4902.0 20 4234.0 21 4105.0 22 4352.0 23 4771.0 24 5502.0 25 6677.0 26 8021.0 27 9684.0 28 11706.0 29 14308.0 30 18010.0 31 22127.0 32 27128.0 33 34940.0 34 46967.0 35 64265.0 36 90629.0 37 114418.0 38 60356.0 39 2510.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.105365242400715 19.718157308014035 15.103762235012214 21.072715214573037 2 15.869797047355616 21.429833038957575 39.56060252607725 23.13976738760956 3 19.625929968091576 26.33486349359749 30.23202339395656 23.807183144354376 4 12.993320481911653 17.845093161699655 36.864142034312295 32.297444322076394 5 13.067884551940631 38.32906229276567 33.4199204886648 15.183132666628898 6 30.262932090177657 36.97727231074005 17.505035880125227 15.25475971895707 7 27.708133397312864 34.34301023672425 20.385310034122416 17.563546331840477 8 27.059292014755528 34.032676754717734 19.33663455418523 19.57139667634151 9 27.27639276032876 16.83408503598009 20.413933518051692 35.47558868563945 10-11 24.89682485467175 27.36119881438138 26.41883527465332 21.323141056293558 12-13 26.503974554851524 25.073268098761563 27.378193347806455 21.044563998580458 14-15 23.135885076583953 25.984394967601983 26.879443377191592 24.000276578622472 16-17 21.250658144665195 29.483298121205255 27.392064274907863 21.87397945922169 18-19 21.086783386428163 26.850682457379737 31.422780347360142 20.63975380883196 20-21 22.210003848787633 25.68374295430273 31.58621700830905 20.520036188600592 22-23 21.741245703451067 25.93832629946134 31.445323238768992 20.875104758318603 24-25 21.683901501109233 26.152490171854055 31.513297050348264 20.650311276688445 26-27 21.41627776398592 26.205237315330237 31.530107815319408 20.84837710536444 28-29 21.08471774912403 26.553208717862418 31.530940880683378 20.83113265233017 30-31 21.129905097035614 26.33848835039454 31.480926103646834 21.05068044892301 32-33 21.04307721274013 26.540968214653766 31.36321337495512 21.052741197650985 34-35 21.620236924973756 26.39789066795515 31.282593845282324 20.699278561788766 36-37 21.876739032639662 26.124227328012793 31.098235558739734 20.900798080607807 38-39 21.62891931634043 26.30664706053878 31.185823603167528 20.87861001995326 40-41 21.466844831579177 26.51530424380158 31.12832039027446 20.889530534344786 42-43 21.500031240237426 26.753889409559513 30.4814745392065 21.264604810996563 44-45 21.676245920937205 26.757818845874116 30.300240682408358 21.265694550780324 46-47 21.90928584638608 27.275976227646776 29.521587222776674 21.293150703190474 48-49 22.18376182657166 27.388078165963964 28.985920162883243 21.442239844581124 50-51 22.100602314286427 27.639561135316608 28.78953989186667 21.470296658530287 52-53 22.231728479088687 27.92634659553358 28.46592617831535 21.375998747062386 54-55 21.74406002084335 27.89493020144404 28.628529394368297 21.732480383344317 56-57 21.964076386210092 28.096451712074543 28.44504023681358 21.494431664901782 58-59 22.168849745369965 27.64808154825724 28.435589389596032 21.747479316776758 60-61 21.944313001519554 27.700874410173014 28.98149876036363 21.373313827943804 62-63 21.765183670239132 27.855396393657035 29.023188393723682 21.35623154238015 64-65 21.512433384401476 27.931523705031086 28.851583320628784 21.70445958993866 66-67 21.615560457723245 28.056209054496257 28.517231267536168 21.810999220244323 68-69 21.701417236771512 27.940358091378947 28.562419399448835 21.795805272400706 70-71 21.72376160087975 28.165686388856493 28.371794658180182 21.738757352083578 72-73 21.806856437355098 27.989996342766883 28.31498190960886 21.888165310269162 74-75 21.799936683551053 28.335943748333776 28.101589576113035 21.762529992002133 76-77 21.773336965513447 28.07516946702755 28.23170310093212 21.919790466526877 78-79 21.743473450590173 28.383325224438654 27.88104584746836 21.992155477502816 80-81 21.81587190509514 28.573011429904362 27.833899830050985 21.777216834949513 82-83 21.81850596660302 28.654190284817403 27.748952803686933 21.778350944892647 84-85 21.63121335468252 28.587887946762546 27.755969469108383 22.02492922944655 86-87 21.664722837768043 28.428747563271628 27.631083490228093 22.275446108732236 88-89 21.578258540555716 28.429614091094262 27.49714460899679 22.494982759353228 90-91 21.507069464960097 28.35600677495599 27.763156469658078 22.373767290425832 92-93 21.522876470392216 28.355493351994404 27.796494385017827 22.325135792595553 94-95 21.342009482005267 28.19842733003493 27.82104651947427 22.638516668485536 96-97 21.514883166456038 28.04863114522615 27.777134464583387 22.659351223734426 98-99 21.181613094563705 28.467163622065083 27.371257006138077 22.97996627723313 100-101 20.768366928698295 29.024195993881857 27.344455515160405 22.86298156225945 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 359.0 1 247.5 2 250.0 3 352.0 4 287.0 5 227.0 6 347.5 7 669.5 8 1038.5 9 1312.0 10 1251.0 11 1106.0 12 1158.0 13 1196.5 14 1268.5 15 1409.0 16 1515.0 17 1573.5 18 1634.5 19 1740.0 20 1864.0 21 1987.5 22 2247.0 23 2577.5 24 2894.0 25 3363.5 26 4106.5 27 5028.5 28 5821.5 29 6803.0 30 8153.5 31 9530.5 32 11048.0 33 12226.0 34 13498.5 35 14488.5 36 15387.5 37 16683.5 38 17148.5 39 17047.0 40 17223.5 41 17967.5 42 18550.0 43 19108.5 44 20012.0 45 20698.5 46 20769.5 47 21226.5 48 23630.5 49 23521.0 50 21286.0 51 20038.5 52 18650.5 53 18024.5 54 17320.5 55 16200.0 56 14806.5 57 13365.0 58 12445.0 59 11211.5 60 9417.0 61 7704.5 62 6378.5 63 4924.5 64 3501.5 65 2763.5 66 2173.0 67 1736.5 68 1257.5 69 846.5 70 640.5 71 460.5 72 383.0 73 331.5 74 274.5 75 224.5 76 168.0 77 103.0 78 61.5 79 38.0 80 26.0 81 21.5 82 14.0 83 6.0 84 5.5 85 3.5 86 2.5 87 1.5 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011829528168439152 2 0.008830492858130635 3 0.00633129676620687 4 0.0 5 0.004498552965462777 6 0.0 7 1.6661307279491763E-4 8 0.001832743800744094 9 0.0 10-11 0.0 12-13 0.0 14-15 3.3322614558983527E-4 16-17 0.0034988745286932705 18-19 0.0014995176551542587 20-21 6.664522911796705E-4 22-23 3.3322614558983527E-4 24-25 5.831457547822116E-4 26-27 0.0 28-29 0.0 30-31 1.6661307279491763E-4 32-33 0.004415246429065317 34-35 4.998392183847529E-4 36-37 4.998392183847529E-4 38-39 0.006747829448194164 40-41 0.0 42-43 9.16371900372047E-4 44-45 0.004082020283475482 46-47 0.0 48-49 0.0014162111187567999 50-51 0.001332904582359341 52-53 5.831457547822116E-4 54-55 2.499196091923764E-4 56-57 0.009746864758502682 58-59 0.0014162111187567999 60-61 0.0028324222375135997 62-63 8.330653639745882E-5 64-65 0.0024158895555263056 66-67 8.330653639745881E-4 68-69 0.001832743800744094 70-71 0.0038321006742831052 72-73 0.0020826634099364704 74-75 0.0054982314022322815 76-77 4.1653268198729406E-4 78-79 0.004831779111052611 80-81 0.0021659699463339294 82-83 0.003165648383103435 84-85 0.0023325830191288466 86-87 4.998392183847529E-4 88-89 0.0020826634099364704 90-91 0.0069144425209890815 92-93 0.0021659699463339294 94-95 5.831457547822116E-4 96-97 2.499196091923764E-4 98-99 8.330653639745881E-4 100-101 0.001832743800744094 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 600193.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.11767663440826 #Duplication Level Percentage of deduplicated Percentage of total 1 84.47391450785533 55.00745048965171 2 9.422466752281446 12.27138286147052 3 2.5118419440796775 4.906959344139715 4 1.1219530273613365 2.922358977388436 5 0.5913660074508877 1.9254190222883987 6 0.37164086285355624 1.4520233718858202 7 0.2519970089017356 1.1486621816950922 8 0.18580854699439273 0.9679536703272092 9 0.1397810492988422 0.8191995451074257 >10 0.8387155824287842 10.172394133525492 >50 0.058975610306914304 2.5962462513827034 >100 0.029487776597676416 3.447299425809243 >500 7.692463460243494E-4 0.2861896355627329 >1k 0.0012820772433739156 2.0764610897655467 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4277 0.7126041123438627 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3791 0.6316301589655328 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2114 0.3522200358884559 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1173 0.1954371343884384 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1081 0.18010873169130595 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 603 0.10046768289533534 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 1.6661307279491763E-4 10-11 0.0 0.0 0.0 0.0 1.6661307279491763E-4 12-13 0.0 1.6661307279491763E-4 0.0 0.0 1.6661307279491763E-4 14-15 0.0 1.6661307279491763E-4 0.0 0.0 1.6661307279491763E-4 16-17 0.0 1.6661307279491763E-4 0.0 0.0 1.6661307279491763E-4 18-19 0.0 1.6661307279491763E-4 0.0 8.330653639745882E-5 1.6661307279491763E-4 20-21 0.0 1.6661307279491763E-4 0.0 4.1653268198729406E-4 3.3322614558983527E-4 22-23 0.0 1.6661307279491763E-4 0.0 4.998392183847529E-4 4.1653268198729406E-4 24-25 0.0 1.6661307279491763E-4 0.0 7.497588275771293E-4 4.998392183847529E-4 26-27 0.0 1.6661307279491763E-4 0.0 0.0014162111187567999 4.998392183847529E-4 28-29 0.0 1.6661307279491763E-4 0.0 0.002165969946333929 4.998392183847529E-4 30-31 0.0 1.6661307279491763E-4 0.0 0.0045818595018602346 4.998392183847529E-4 32-33 0.0 1.6661307279491763E-4 0.0 0.007664201348566211 4.998392183847529E-4 34-35 8.330653639745882E-5 1.6661307279491763E-4 0.0 0.011079769340862022 4.998392183847529E-4 36-37 1.6661307279491763E-4 1.6661307279491763E-4 0.0 0.014995176551542588 4.998392183847529E-4 38-39 1.6661307279491763E-4 1.6661307279491763E-4 0.0 0.021992925608929126 4.998392183847529E-4 40-41 1.6661307279491763E-4 1.6661307279491763E-4 0.0 0.027491157011161407 4.998392183847529E-4 42-43 1.6661307279491763E-4 1.6661307279491763E-4 0.0 0.04123673551674212 4.998392183847529E-4 44-45 1.6661307279491763E-4 1.6661307279491763E-4 0.0 0.059730786596977975 4.998392183847529E-4 46-47 3.3322614558983527E-4 1.6661307279491763E-4 0.0 0.07339305856616121 4.998392183847529E-4 48-49 3.3322614558983527E-4 1.6661307279491763E-4 0.0 0.09080412467323012 4.998392183847529E-4 50-51 3.3322614558983527E-4 1.6661307279491763E-4 0.0 0.11196398491818466 4.998392183847529E-4 52-53 3.3322614558983527E-4 1.6661307279491763E-4 0.0 0.15611644920883783 5.831457547822117E-4 54-55 3.3322614558983527E-4 1.6661307279491763E-4 0.0 0.1889392245494366 6.664522911796705E-4 56-57 3.3322614558983527E-4 1.6661307279491763E-4 0.0 0.24242202091660514 6.664522911796705E-4 58-59 3.3322614558983527E-4 1.6661307279491763E-4 0.0 0.3193139540114597 6.664522911796705E-4 60-61 3.3322614558983527E-4 1.6661307279491763E-4 0.0 0.38954136419451746 6.664522911796705E-4 62-63 3.3322614558983527E-4 1.6661307279491763E-4 0.0 0.4892592882622756 6.664522911796705E-4 64-65 3.3322614558983527E-4 1.6661307279491763E-4 0.0 0.5619858945372571 6.664522911796705E-4 66-67 4.998392183847529E-4 1.6661307279491763E-4 0.0 0.6911110259533184 6.664522911796705E-4 68-69 4.998392183847529E-4 1.6661307279491763E-4 0.0 0.8542252242195427 6.664522911796705E-4 70-71 5.831457547822117E-4 1.6661307279491763E-4 0.0 0.959024847007546 6.664522911796705E-4 72-73 6.664522911796705E-4 1.6661307279491763E-4 0.0 1.1602934389438064 6.664522911796705E-4 74-75 6.664522911796705E-4 1.6661307279491763E-4 0.0 1.3446508039913827 6.664522911796705E-4 76-77 6.664522911796705E-4 1.6661307279491763E-4 0.0 1.4613632614842227 6.664522911796705E-4 78-79 6.664522911796705E-4 1.6661307279491763E-4 0.0 1.5836572569156921 6.664522911796705E-4 80-81 6.664522911796705E-4 1.6661307279491763E-4 0.0 1.8132500712270887 6.664522911796705E-4 82-83 6.664522911796705E-4 1.6661307279491763E-4 0.0 2.00268913499491 6.664522911796705E-4 84-85 6.664522911796705E-4 1.6661307279491763E-4 0.0 2.2882639417653987 6.664522911796705E-4 86-87 6.664522911796705E-4 1.6661307279491763E-4 0.0 2.712294212028464 6.664522911796705E-4 88-89 6.664522911796705E-4 1.6661307279491763E-4 0.0 3.2808613229411208 6.664522911796705E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3840 0.0 29.82192 1 GGTAAGG 305 0.0 29.59341 3 GTATCAA 5485 0.0 29.021374 1 GTACCTG 605 0.0 28.27463 1 TACCTGG 575 0.0 28.094717 2 GTAAGGT 320 0.0 26.717226 4 AGGTAAG 350 0.0 24.435322 2 GGTGTGC 585 0.0 24.357586 8 TAAGGTG 375 0.0 24.065294 5 GCACCGT 140 1.778428E-5 23.748646 6 ATCAACG 6715 0.0 23.628801 3 TCAACGC 6765 0.0 23.450253 4 GAGTACT 3820 0.0 23.375631 12-13 TATCAAC 6805 0.0 23.320183 2 CAACGCA 6890 0.0 23.09375 5 AACGCAG 6990 0.0 22.831318 6 GTACATG 3695 0.0 21.60452 1 AGTACTT 3955 0.0 21.556925 12-13 GTACTTT 4185 0.0 21.223402 14-15 CCTACCG 80 4.9901137E-5 20.780931 90-91 >>END_MODULE