FastQCFastQC Report
Thu 26 May 2016
SRR936638_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936638_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682127
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47330.6938590614357737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38180.5597198175706283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21920.3213477842102717No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11010.16140689343773226No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7520.11024340042250197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG407.518962E-659.4059072
ATCACGC651.3229882E-436.5601653
GTATCAA54800.032.0902181
GGTATCA36300.031.9473381
GTACACG902.4017669E-531.6854721
TATCACG650.005827588529.2459832
TACCTGG6200.027.5950012
TATCAAC66550.026.422462
ATCAACG66900.026.001983
GTACCTG7150.025.9244791
TCAACGC67400.025.810984
CAACGCA67850.025.503435
AACGCAG69050.025.0583746
TAACGCA1000.001646551923.7658484
GAGTACT37700.023.63543512-13
GTACATG44900.022.6526451
ACGCAGA77250.022.3352817
CGCAGAG77550.022.248888
TACATGG45750.021.7107472
GTACTTT41600.021.53069114-15
Produced by FastQC (version 0.11.5)