##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936638_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 682127 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.399902071021966 31.0 31.0 34.0 30.0 34.0 2 31.4391924084518 31.0 31.0 34.0 28.0 34.0 3 31.45349473045342 31.0 31.0 34.0 28.0 34.0 4 35.114456692082264 37.0 35.0 37.0 32.0 37.0 5 35.047365080109714 37.0 35.0 37.0 32.0 37.0 6 35.17674714532631 37.0 35.0 37.0 32.0 37.0 7 35.00710571491819 37.0 35.0 37.0 32.0 37.0 8 35.00455926828875 37.0 35.0 37.0 32.0 37.0 9 36.50911487157084 39.0 37.0 39.0 32.0 39.0 10-11 36.38298659926964 39.0 37.0 39.0 32.0 39.0 12-13 36.287122485988675 39.0 35.0 39.0 32.0 39.0 14-15 37.33801623451352 40.0 37.0 41.0 32.0 41.0 16-17 37.28383937301998 39.0 37.0 41.0 32.0 41.0 18-19 37.303561506874814 39.5 37.0 41.0 32.0 41.0 20-21 37.24092287799779 39.5 37.0 41.0 31.0 41.0 22-23 37.14887990066366 39.0 36.0 41.0 31.0 41.0 24-25 36.99875243173192 39.0 36.0 41.0 31.0 41.0 26-27 36.7923377904701 39.0 36.0 41.0 30.5 41.0 28-29 36.87069270091933 39.0 36.0 41.0 30.0 41.0 30-31 36.77050094777073 39.0 36.0 41.0 30.0 41.0 32-33 36.57240147362588 39.0 35.0 41.0 30.0 41.0 34-35 36.377061016496924 39.0 35.0 40.5 30.0 41.0 36-37 36.11276345900397 38.5 35.0 40.0 29.5 41.0 38-39 35.920981723344774 38.0 35.0 40.0 29.0 41.0 40-41 35.748697090131316 38.0 35.0 40.0 28.5 41.0 42-43 35.4572674883123 38.0 35.0 40.0 27.0 41.0 44-45 35.105497216793935 38.0 34.5 40.0 25.5 41.0 46-47 34.64956965491763 38.0 34.0 40.0 24.0 41.0 48-49 34.2856117702422 38.0 33.0 40.0 23.0 41.0 50-51 33.538387279788076 37.5 32.5 39.5 21.5 40.5 52-53 33.7087191974515 38.0 33.0 39.5 22.0 40.5 54-55 34.048943964980126 38.0 34.0 40.0 21.5 41.0 56-57 33.98491923058316 38.0 34.0 40.0 21.0 41.0 58-59 33.76332852973127 38.0 33.0 40.0 20.0 41.0 60-61 33.4563996147345 37.5 33.0 40.0 18.5 41.0 62-63 33.138695580148564 37.0 33.0 40.0 18.0 41.0 64-65 32.83349654829672 36.5 32.0 39.5 16.0 41.0 66-67 32.47922307722756 36.0 32.0 39.0 13.0 41.0 68-69 32.1066025828035 35.5 31.5 39.0 9.5 40.5 70-71 31.71958520920591 35.0 31.0 38.5 8.0 40.0 72-73 31.309437245556914 35.0 31.0 37.5 7.0 40.0 74-75 30.886092325915847 35.0 31.0 37.0 4.0 39.0 76-77 30.47965554801379 35.0 30.0 36.5 2.0 39.0 78-79 30.034462790653354 34.5 30.0 36.0 2.0 39.0 80-81 29.63215867426447 34.0 29.0 36.0 2.0 37.5 82-83 29.230616879261486 34.0 29.0 35.0 2.0 37.0 84-85 28.849566869512568 34.0 29.0 35.0 2.0 36.5 86-87 28.518174768041728 34.0 29.0 35.0 2.0 36.0 88-89 28.150786437129746 34.0 27.5 35.0 2.0 36.0 90-91 27.80694284202209 34.0 27.0 35.0 2.0 35.5 92-93 27.444557978206404 34.0 26.5 35.0 2.0 35.0 94-95 27.040119361937002 33.5 26.0 35.0 2.0 35.0 96-97 26.491783788062925 33.0 25.0 35.0 2.0 35.0 98-99 25.853461305592653 33.0 23.5 35.0 2.0 35.0 100-101 23.851639797281152 31.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2601132245792712 1101 2 0.33584043224234605 1101 3 0.31592211265317616 1101 4 0.3378047309865124 1101 5 0.4029407181725375 1101 6 0.4477640923803463 1101 7 0.3711741517199414 1101 8 0.313732564725143 1101 9 0.5124292472304646 1101 10-11 0.5149219053155036 1101 12-13 0.6162738244985988 1101 14-15 0.5840939089102406 1101 16-17 0.5474346912148391 1101 18-19 0.6661418447467824 1101 20-21 0.5119644028987622 1101 22-23 0.4959141088283374 1101 24-25 0.5427254461654982 1101 26-27 0.4612798398812217 1101 28-29 0.6009142031186343 1101 30-31 0.23936531116171267 1101 32-33 0.35707449022637405 1101 34-35 0.265798961769967 1101 36-37 0.611188454119926 1101 38-39 0.33771536872891517 1101 40-41 0.1650127846472671 1101 42-43 0.18308723147753625 1101 44-45 0.37731515058337806 1101 46-47 0.5110383142650363 1101 48-49 0.2763692078508484 1101 50-51 0.16133564164046277 1101 52-53 0.33775935205508034 1101 54-55 0.538920619104772 1101 56-57 0.6082490533663076 1101 58-59 0.3419294905970247 1101 60-61 0.23083012913681245 1101 62-63 0.23688837650553296 1101 64-65 0.32994135224389964 1101 66-67 0.1994793965438859 1101 68-69 0.08648276456678161 1101 70-71 0.21995424019521437 1101 72-73 0.17174001526326776 1101 74-75 0.19504056299269834 1101 76-77 0.2042191996245215 1101 78-79 0.11994057061138008 1101 80-81 0.013437437013845255 1101 82-83 0.08737431522417083 1101 84-85 0.15891161418271693 1101 86-87 0.12252890660039739 1101 88-89 0.3066535945348221 1101 90-91 0.4768152957068672 1101 92-93 0.21571082366732597 1101 94-95 0.17025290077723554 1101 96-97 0.12190967751608994 1101 98-99 0.004669307766857145 1101 100-101 0.5944555415712571 1102 1 0.0696399785958377 1102 2 0.28945294410281264 1102 3 0.03894368004728577 1102 4 0.35871007112873343 1102 5 0.13738671439851657 1102 6 0.08556513503538099 1102 7 0.04332630686018746 1102 8 -0.049461072733677725 1102 9 0.3457558285372997 1102 10-11 0.4042709388872936 1102 12-13 0.325740876386142 1102 14-15 0.29972094118136994 1102 16-17 0.28550865788825774 1102 18-19 0.3079711819465487 1102 20-21 0.21476773768911528 1102 22-23 -0.0022372573909237303 1102 24-25 0.2500917293881315 1102 26-27 0.2737341304386902 1102 28-29 0.1193013927475306 1102 30-31 -0.14290902012113804 1102 32-33 -0.18103252583990326 1102 34-35 -0.16566725855221165 1102 36-37 -0.3164296441540415 1102 38-39 -0.10061393115700668 1102 40-41 -0.25268409911482337 1102 42-43 -0.3125507817209012 1102 44-45 -0.008585018928023658 1102 46-47 -0.04808811565425941 1102 48-49 0.009049193778210451 1102 50-51 0.4374100134354677 1102 52-53 0.15207933414443886 1102 54-55 -0.023581646377792254 1102 56-57 0.19415828480801167 1102 58-59 0.2199937556744871 1102 60-61 0.1665026842813191 1102 62-63 0.27918093825991974 1102 64-65 0.34880829112384504 1102 66-67 -0.010626254902291521 1102 68-69 0.0858276403117273 1102 70-71 0.38297676708782546 1102 72-73 0.3634344896180046 1102 74-75 0.34049200703794114 1102 76-77 0.3400959147280993 1102 78-79 0.35969419271822645 1102 80-81 0.3306865548082776 1102 82-83 0.23195261563249048 1102 84-85 0.19963166450308378 1102 86-87 0.38654611360686886 1102 88-89 0.1667379237920663 1102 90-91 -0.16616384053126154 1102 92-93 -0.005209975061873706 1102 94-95 -0.0071082651200526925 1102 96-97 0.05341854221614284 1102 98-99 -0.08323727972341644 1102 100-101 0.3424267352940049 1103 1 0.14400694243296286 1103 2 0.3850240002858314 1103 3 0.21621858863188592 1103 4 0.19626664954545703 1103 5 0.24930327235801997 1103 6 0.2695460926947888 1103 7 0.14332337796255246 1103 8 0.00624205420249524 1103 9 0.38279737157069604 1103 10-11 0.3998263367329855 1103 12-13 0.4036163216349067 1103 14-15 0.45558617133639956 1103 16-17 0.6110916774915935 1103 18-19 0.3512349426961592 1103 20-21 0.3317766347789117 1103 22-23 0.4234582994713705 1103 24-25 0.39011331311066755 1103 26-27 0.45599717698788567 1103 28-29 0.3336961360521826 1103 30-31 0.25651038224856393 1103 32-33 0.41583513079417145 1103 34-35 0.31396620117431695 1103 36-37 0.5840103663899896 1103 38-39 0.42869194263453636 1103 40-41 0.5085240035678211 1103 42-43 0.5907989759691503 1103 44-45 0.5310536677542785 1103 46-47 0.5122680157676314 1103 48-49 0.47247683761524684 1103 50-51 0.09384243588164054 1103 52-53 0.39613479845356636 1103 54-55 0.5182728640119052 1103 56-57 0.5747921883751843 1103 58-59 0.6927223392370578 1103 60-61 0.6557970001922158 1103 62-63 0.4253920712251187 1103 64-65 0.5834653735362707 1103 66-67 0.7334472333922477 1103 68-69 0.5509191233027124 1103 70-71 0.7044948147297276 1103 72-73 0.6845086966189697 1103 74-75 0.6467037483126425 1103 76-77 0.6462761140283142 1103 78-79 0.9314043084547414 1103 80-81 0.8749314962641215 1103 82-83 0.9141627167245154 1103 84-85 0.4572293376065666 1103 86-87 0.46106966081731926 1103 88-89 0.6583167798547684 1103 90-91 0.8099442402973054 1103 92-93 0.5598352088165726 1103 94-95 0.6174464861151527 1103 96-97 0.8562930299406801 1103 98-99 0.8910909325450618 1103 100-101 0.6743333575315447 1104 1 0.2537548763675552 1104 2 0.31302755428346885 1104 3 0.37407450567281586 1104 4 0.48013612533080874 1104 5 0.36409374472324885 1104 6 0.5101553842077919 1104 7 0.24075890089923035 1104 8 0.31238487135418325 1104 9 0.3824517087866468 1104 10-11 0.4059977562629271 1104 12-13 0.4334425165449076 1104 14-15 0.33804195936235004 1104 16-17 0.18703038320355603 1104 18-19 0.49879461212569964 1104 20-21 0.3329371610626808 1104 22-23 0.2592556972635194 1104 24-25 0.2601331522070822 1104 26-27 0.22896975521843643 1104 28-29 -0.029800535117843197 1104 30-31 0.08631284327022115 1104 32-33 0.03502184955367227 1104 34-35 0.06446278714576437 1104 36-37 0.3590459672224995 1104 38-39 0.3599379109163223 1104 40-41 0.01437349420206857 1104 42-43 0.22828391407846738 1104 44-45 0.09392230372511534 1104 46-47 0.4849137966125525 1104 48-49 0.475295084715448 1104 50-51 0.2944059635894334 1104 52-53 0.47763149396603666 1104 54-55 0.8975884760419461 1104 56-57 0.885741422196574 1104 58-59 0.8946709523259884 1104 60-61 0.5854491465877132 1104 62-63 0.6278634384187995 1104 64-65 0.6876041985262304 1104 66-67 0.7209273030116563 1104 68-69 0.6045412798003227 1104 70-71 0.5781325503950683 1104 72-73 0.35298461585072616 1104 74-75 0.13391057393550554 1104 76-77 0.5101628729528649 1104 78-79 0.5389839386929332 1104 80-81 0.6843842779975446 1104 82-83 0.3959557815606338 1104 84-85 0.7437241465791935 1104 86-87 0.8690272075852512 1104 88-89 0.5418923205614341 1104 90-91 0.7566805263697702 1104 92-93 0.6327384110658123 1104 94-95 0.46611191131689367 1104 96-97 0.45624130223532333 1104 98-99 0.3843991931553248 1104 100-101 0.20082828821642096 1105 1 0.23224096841950725 1105 2 0.2628509815504607 1105 3 0.33686350938637233 1105 4 0.5484824550242919 1105 5 0.4561925213961402 1105 6 0.17405455239738643 1105 7 0.3804080967291199 1105 8 0.05563576072145082 1105 9 0.3734208101842711 1105 10-11 0.3810675073701617 1105 12-13 0.226310669067324 1105 14-15 0.49610739858295716 1105 16-17 0.5422524486167575 1105 18-19 0.3238972064116368 1105 20-21 0.0021861904004154553 1105 22-23 -0.15525903664170215 1105 24-25 -0.11809149513522499 1105 26-27 -0.0773673523706151 1105 28-29 -0.022630316401780703 1105 30-31 0.15858149396838428 1105 32-33 -0.06368328373760335 1105 34-35 -0.12061535360513886 1105 36-37 -0.20807573463947904 1105 38-39 -0.11278989392365446 1105 40-41 -0.36906964157358146 1105 42-43 -0.022305637238190457 1105 44-45 0.24446047660379833 1105 46-47 0.049473080176007045 1105 48-49 0.39168610714636287 1105 50-51 0.325089947174348 1105 52-53 0.4466704570320914 1105 54-55 0.4786545461117271 1105 56-57 0.5869992183867723 1105 58-59 0.6449112603364782 1105 60-61 0.7650889772339795 1105 62-63 0.557826172792268 1105 64-65 0.6635155154401389 1105 66-67 0.6780939447478644 1105 68-69 0.5830605402109583 1105 70-71 0.7342534132926772 1105 72-73 0.5420969395218762 1105 74-75 0.4541874972125406 1105 76-77 0.2845835648058035 1105 78-79 0.4873967351757855 1105 80-81 0.6500569778369076 1105 82-83 0.42249306994975555 1105 84-85 0.0808239313100394 1105 86-87 0.485476112053874 1105 88-89 0.5161497720608352 1105 90-91 0.5278624255509676 1105 92-93 0.5378001627453699 1105 94-95 0.5756019640610859 1105 96-97 0.8884471281599708 1105 98-99 0.993365706120251 1105 100-101 1.0626293951290933 1106 1 0.05313576769804129 1106 2 0.11130042709383758 1106 3 -0.11337658673513218 1106 4 0.04204367088378547 1106 5 0.13777896915014765 1106 6 0.013049650809804803 1106 7 0.3708873061629774 1106 8 0.3102193195702583 1106 9 0.1254525258506618 1106 10-11 0.1962800087024945 1106 12-13 0.22429937091487062 1106 14-15 0.03889489448214789 1106 16-17 0.11087536689548472 1106 18-19 -0.0034114607192137214 1106 20-21 0.10621584632031755 1106 22-23 0.13487729698186257 1106 24-25 0.04450597253934774 1106 26-27 -0.04268941615908517 1106 28-29 -0.060134867084968846 1106 30-31 0.09983027064784267 1106 32-33 0.07463949016507598 1106 34-35 0.029417383629073868 1106 36-37 -0.0978343096492722 1106 38-39 0.10564566771024886 1106 40-41 -0.15276857529487842 1106 42-43 -0.12562171834900937 1106 44-45 -0.22389168467655907 1106 46-47 -0.14893324634640948 1106 48-49 0.08084779863011704 1106 50-51 0.16365247113896686 1106 52-53 -0.041266598886984696 1106 54-55 0.2352265631821524 1106 56-57 -0.01875242989490289 1106 58-59 0.061656251816494034 1106 60-61 -0.048955227029757964 1106 62-63 0.09844249102310698 1106 64-65 -0.23782774934110762 1106 66-67 0.11695491047550632 1106 68-69 -0.1599390209855187 1106 70-71 -4.5040817380836984E-4 1106 72-73 -0.06982010584930265 1106 74-75 -0.18781735038975356 1106 76-77 -0.26142920617613186 1106 78-79 -0.17055246051258877 1106 80-81 -0.5192194393115912 1106 82-83 -0.183481440687423 1106 84-85 -0.05133759821783812 1106 86-87 0.2534438703769517 1106 88-89 0.07676833275760586 1106 90-91 0.016392627552853867 1106 92-93 0.034250975916119586 1106 94-95 -0.07095356673198694 1106 96-97 0.06016188095578556 1106 98-99 0.6981962822817316 1106 100-101 0.472446274253258 1107 1 0.37821173546128506 1107 2 0.4988020785669498 1107 3 0.5263380387107652 1107 4 0.4077458772518696 1107 5 0.404062494054358 1107 6 0.3651437187183788 1107 7 0.6758153019352093 1107 8 0.4904280657718729 1107 9 0.7521535431581015 1107 10-11 0.6201140686839963 1107 12-13 0.6957619155046402 1107 14-15 0.7977467903732887 1107 16-17 0.64925560206526 1107 18-19 0.8032670965538742 1107 20-21 0.810271864288346 1107 22-23 0.7946738354942156 1107 24-25 0.8903574720769356 1107 26-27 0.8416744363973976 1107 28-29 0.9164472441582561 1107 30-31 0.7254082862377516 1107 32-33 0.4946421325220527 1107 34-35 0.44615010922029086 1107 36-37 0.5837286445058254 1107 38-39 0.5789248327254413 1107 40-41 0.725055443730092 1107 42-43 0.6458474321332233 1107 44-45 0.488983199720316 1107 46-47 0.7773119643815605 1107 48-49 0.7739380351210627 1107 50-51 0.680068113892311 1107 52-53 0.5075839758256606 1107 54-55 0.6806949209572863 1107 56-57 0.8612845631028492 1107 58-59 0.616187894041353 1107 60-61 0.7358247967514515 1107 62-63 0.6553522545321542 1107 64-65 0.5511800456119929 1107 66-67 0.5700861034996834 1107 68-69 0.8206396551936308 1107 70-71 1.014313761465509 1107 72-73 0.7318379731571767 1107 74-75 0.5334139879640603 1107 76-77 0.45260170940056454 1107 78-79 0.5462529298956547 1107 80-81 0.7074947897807604 1107 82-83 1.0615971694328046 1107 84-85 1.116815394251443 1107 86-87 0.9481855799819208 1107 88-89 1.1599695482418149 1107 90-91 0.8760174128613443 1107 92-93 0.962246748144949 1107 94-95 1.0111246470346913 1107 96-97 0.7908396455217819 1107 98-99 0.7661378861924177 1107 100-101 0.969671799421711 1108 1 0.2251213460364916 1108 2 0.05549104383779735 1108 3 0.2769755816756181 1108 4 0.3088558795926062 1108 5 0.37926915653233806 1108 6 0.11403186842701984 1108 7 0.4252763163369053 1108 8 0.3906510518136841 1108 9 0.4527978619419031 1108 10-11 0.2818990657888776 1108 12-13 0.5372354713312362 1108 14-15 0.6022859730863175 1108 16-17 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-3.587625947409027 2316 92-93 -3.90311824039426 2316 94-95 -3.9536355129144454 2316 96-97 -4.185393853449259 2316 98-99 -4.681213583633074 2316 100-101 -5.206104941029025 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1560.0 3 602.0 4 580.0 5 627.0 6 721.0 7 816.0 8 1094.0 9 1392.0 10 1708.0 11 2422.0 12 3112.0 13 3840.0 14 4471.0 15 5496.0 16 7162.0 17 9430.0 18 8018.0 19 4900.0 20 4616.0 21 4780.0 22 5181.0 23 5943.0 24 6677.0 25 7730.0 26 9053.0 27 10554.0 28 12436.0 29 14966.0 30 18119.0 31 22384.0 32 27767.0 33 35373.0 34 47404.0 35 64695.0 36 90248.0 37 120349.0 38 98810.0 39 17085.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.18281972558714 18.492518012192868 14.8236608517414 21.501001410478597 2 15.434292433052141 20.161765611393083 41.04278270864304 23.361159246911733 3 19.153605934874722 25.901593678068235 30.433386602548122 24.51141378450892 4 12.475251590497784 16.83067834840481 37.871152813912495 32.82291724718491 5 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21.795629234023775 50-51 21.732032970859127 27.11233768375683 29.419198305277316 21.736431040106726 52-53 21.954194444546253 27.22619470842847 29.147623777254083 21.67198706977119 54-55 21.5195394277739 27.08456725327954 29.32790701765703 22.067986301289533 56-57 21.737721765429846 27.359236723028417 29.029198305555614 21.87384320598612 58-59 21.99006762081035 26.99430079349081 29.013102677344282 22.00252890835456 60-61 21.75418252399857 26.973394866622492 29.581351777682652 21.69107083169629 62-63 21.70014073770011 27.090834457280245 29.514968040813933 21.694056764205712 64-65 21.46696104992787 27.251636114140954 29.346404653835776 21.9349981820954 66-67 21.518208859822348 27.420310124568804 29.00267257771073 22.05880843789812 68-69 21.461600262722698 27.330710993302915 29.254436377710796 21.95325236626359 70-71 21.524012130870894 27.332359325368756 28.992546859777306 22.151081683983044 72-73 21.59661339979475 27.250329863656354 28.965107755461077 22.187948981087818 74-75 21.43916892195009 27.714036903571603 28.737124359699866 22.109669814778435 76-77 21.56217976253132 27.452056923286676 28.813052421086894 22.17271089309511 78-79 21.55542454732501 27.462307025609668 28.75635548790056 22.225912939164765 80-81 21.520531634729874 27.681635023138778 28.603593695928865 22.194239646202483 82-83 21.53511215364316 27.65591555490397 28.712358891658113 22.09661339979475 84-85 21.501745764240095 27.622733127150134 28.387380063467678 22.4881410451421 86-87 21.685484024671485 27.552658547420183 28.41890209842497 22.342955329483363 88-89 21.642436011057185 27.335595496492033 28.357600640970666 22.66436785148012 90-91 21.50354366611833 27.227216880576837 28.81416938636077 22.45507006694406 92-93 21.62313337096526 27.31102786473033 28.75726674926141 22.308572015042998 94-95 21.270988768360823 27.136994917100505 28.863963899245114 22.728052415293558 96-97 21.327639199507995 27.00938491186481 28.876251547597843 22.78672434102936 98-99 21.16491415349288 27.36266685044716 28.572832062266396 22.899586933793568 100-101 21.033738549888422 27.40913427586455 28.714544162542268 22.84258301170476 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 194.0 1 153.5 2 262.0 3 375.5 4 291.5 5 255.5 6 396.0 7 762.0 8 1237.0 9 1522.0 10 1379.0 11 1179.0 12 1211.0 13 1289.5 14 1403.5 15 1455.0 16 1498.5 17 1622.0 18 1696.0 19 1777.0 20 1934.0 21 2193.5 22 2503.5 23 2806.0 24 3225.0 25 3780.0 26 4563.0 27 5545.5 28 6442.5 29 7636.5 30 9198.0 31 10842.0 32 12600.0 33 13963.5 34 15180.0 35 16294.0 36 17618.5 37 18875.5 38 19215.5 39 19302.5 40 19471.0 41 20325.5 42 21172.5 43 21846.5 44 23019.5 45 24041.5 46 24129.0 47 24564.5 48 26947.0 49 26694.5 50 24146.0 51 22611.0 52 21133.0 53 20660.0 54 20084.5 55 18490.5 56 17068.5 57 15633.0 58 14270.5 59 12669.0 60 10608.0 61 8797.0 62 7250.0 63 5646.0 64 4142.0 65 3204.5 66 2476.0 67 1942.0 68 1482.5 69 985.5 70 713.0 71 550.5 72 424.0 73 349.5 74 280.0 75 239.5 76 167.5 77 100.0 78 65.5 79 39.0 80 33.0 81 21.5 82 12.5 83 7.0 84 4.5 85 5.5 86 4.5 87 4.5 88 2.5 89 1.0 90 1.0 91 2.0 92 2.0 93 1.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013047423720216323 2 0.004251407729059251 3 0.008356215191599218 4 0.0388490706276104 5 0.046325684220093914 6 0.061278911405060935 7 0.030492855436011185 8 0.026094847440432646 9 0.017005630916237004 10-11 0.049184389417219955 12-13 0.04713198568594998 14-15 0.01898473451424735 16-17 0.023089541976787312 18-19 0.03166565756816546 20-21 0.02558174650761515 22-23 0.016199329450380943 24-25 0.003738306796241756 26-27 0.003591706529722471 28-29 0.002418904397568195 30-31 0.006743612259887089 32-33 0.0011728021321542762 34-35 0.0023456042643085523 36-37 0.005424209861213528 38-39 0.005131009328174959 40-41 0.0028587051971260486 42-43 6.597011993367804E-4 44-45 8.796015991157072E-4 46-47 0.002492204530827837 48-49 5.864010660771381E-4 50-51 0.001392702531933203 52-53 6.597011993367804E-4 54-55 0.002785405063866406 56-57 0.00227230413104891 58-59 0.0021257038645296255 60-61 4.398007995578536E-4 62-63 0.0010262018656349917 64-65 0.0033718061299435444 66-67 0.0020524037312699833 68-69 0.005717410394252097 70-71 0.009602317457013137 72-73 0.003958207196020682 74-75 0.003665006662982113 76-77 0.0023456042643085523 78-79 0.034597662898551144 80-81 0.022723041310489103 82-83 0.003958207196020682 84-85 0.006963512659666015 86-87 0.006450411726848519 88-89 0.005350909727953886 90-91 0.009529017323753495 92-93 0.012021221854581331 94-95 0.0052776095946942434 96-97 0.0031519057301646176 98-99 0.022576441043969817 100-101 0.04551938275423785 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 682127.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.52655158773821 #Duplication Level Percentage of deduplicated Percentage of total 1 85.84697196982296 57.96949981371367 2 8.662343824953469 11.69876414332892 3 2.242307612091985 4.542459019305226 4 0.9638390741380815 2.6033891584825204 5 0.5437039954288145 1.8357227947891612 6 0.3428989296255638 1.389286935644451 7 0.22215429056755073 1.050091921371297 8 0.1769741108491836 0.9560361140761198 9 0.1288270861562696 0.78293239913064 >10 0.7934542532066889 9.841074302963184 >50 0.05029408513873173 2.38127877924069 >100 0.024496502366734504 2.846333738582044 >500 8.671328270131705E-4 0.36933244300520013 >1k 8.671328270131705E-4 1.733798436366861 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4733 0.6938590614357737 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3818 0.5597198175706283 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2192 0.3213477842102717 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1101 0.16140689343773226 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 752 0.11024340042250197 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 7.330013325964226E-5 0.0 0.0 0.0 0.0 12-13 1.4660026651928452E-4 0.0 0.0 1.4660026651928452E-4 0.0 14-15 1.4660026651928452E-4 0.0 0.0 1.4660026651928452E-4 0.0 16-17 1.4660026651928452E-4 0.0 0.0 1.4660026651928452E-4 0.0 18-19 1.4660026651928452E-4 0.0 0.0 1.4660026651928452E-4 0.0 20-21 1.4660026651928452E-4 0.0 0.0 2.199003997789268E-4 7.330013325964226E-5 22-23 1.4660026651928452E-4 0.0 0.0 4.398007995578536E-4 2.9320053303856904E-4 24-25 1.4660026651928452E-4 0.0 0.0 8.796015991157072E-4 5.131009328174958E-4 26-27 1.4660026651928452E-4 0.0 0.0 0.0017592031982314143 5.864010660771381E-4 28-29 1.4660026651928452E-4 0.0 0.0 0.002418904397568195 5.864010660771381E-4 30-31 1.4660026651928452E-4 0.0 0.0 0.0040315073292803245 5.864010660771381E-4 32-33 1.4660026651928452E-4 0.0 0.0 0.006083911060550308 5.864010660771381E-4 34-35 1.4660026651928452E-4 0.0 0.0 0.008063014658560649 5.864010660771381E-4 36-37 1.4660026651928452E-4 0.0 0.0 0.011288220521984908 5.864010660771381E-4 38-39 1.4660026651928452E-4 0.0 0.0 0.017738632248833427 5.864010660771381E-4 40-41 1.4660026651928452E-4 0.0 0.0 0.021916739844633035 5.864010660771381E-4 42-43 2.9320053303856904E-4 0.0 0.0 0.03247195903402152 5.864010660771381E-4 44-45 4.398007995578536E-4 0.0 0.0 0.04691208528617105 5.864010660771381E-4 46-47 4.398007995578536E-4 0.0 0.0 0.05856680647445417 5.864010660771381E-4 48-49 4.398007995578536E-4 0.0 0.0 0.07476613592483511 5.864010660771381E-4 50-51 4.398007995578536E-4 0.0 0.0 0.09279796870670712 5.864010660771381E-4 52-53 4.398007995578536E-4 0.0 0.0 0.12424372587509364 5.864010660771381E-4 54-55 4.398007995578536E-4 0.0 0.0 0.1526108774465752 5.864010660771381E-4 56-57 4.398007995578536E-4 0.0 0.0 0.19637105700258162 5.864010660771381E-4 58-59 4.398007995578536E-4 0.0 0.0 0.2615348754704036 5.864010660771381E-4 60-61 4.398007995578536E-4 0.0 0.0 0.3248661906067345 5.864010660771381E-4 62-63 4.398007995578536E-4 0.0 0.0 0.41231324958548776 5.864010660771381E-4 64-65 4.398007995578536E-4 0.0 0.0 0.470660155660163 5.864010660771381E-4 66-67 4.398007995578536E-4 0.0 0.0 0.5798773542170299 5.864010660771381E-4 68-69 4.398007995578536E-4 0.0 0.0 0.7134301970160981 5.864010660771381E-4 70-71 4.398007995578536E-4 0.0 0.0 0.7977986503979464 5.864010660771381E-4 72-73 5.131009328174958E-4 0.0 0.0 0.950116327311483 5.864010660771381E-4 74-75 5.864010660771381E-4 0.0 0.0 1.0975961954298832 5.864010660771381E-4 76-77 5.864010660771381E-4 0.0 0.0 1.1900276634702922 5.864010660771381E-4 78-79 5.864010660771381E-4 0.0 0.0 1.2996113626934576 5.864010660771381E-4 80-81 7.330013325964227E-4 0.0 0.0 1.4835946971751595 5.864010660771381E-4 82-83 7.330013325964227E-4 0.0 0.0 1.6465408934113444 5.864010660771381E-4 84-85 7.330013325964227E-4 0.0 0.0 1.8973739494258401 5.864010660771381E-4 86-87 7.330013325964227E-4 0.0 0.0 2.2574975041304626 5.864010660771381E-4 88-89 7.330013325964227E-4 0.0 0.0 2.764954326686966 5.864010660771381E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 40 7.518962E-6 59.405907 2 ATCACGC 65 1.3229882E-4 36.560165 3 GTATCAA 5480 0.0 32.090218 1 GGTATCA 3630 0.0 31.947338 1 GTACACG 90 2.4017669E-5 31.685472 1 TATCACG 65 0.0058275885 29.245983 2 TACCTGG 620 0.0 27.595001 2 TATCAAC 6655 0.0 26.42246 2 ATCAACG 6690 0.0 26.00198 3 GTACCTG 715 0.0 25.924479 1 TCAACGC 6740 0.0 25.81098 4 CAACGCA 6785 0.0 25.50343 5 AACGCAG 6905 0.0 25.058374 6 TAACGCA 100 0.0016465519 23.765848 4 GAGTACT 3770 0.0 23.635435 12-13 GTACATG 4490 0.0 22.652645 1 ACGCAGA 7725 0.0 22.335281 7 CGCAGAG 7755 0.0 22.24888 8 TACATGG 4575 0.0 21.710747 2 GTACTTT 4160 0.0 21.530691 14-15 >>END_MODULE