FastQCFastQC Report
Thu 26 May 2016
SRR936638_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936638_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682127
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46800.6860892473102516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41880.6139619161827636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24520.3594638535052857No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12320.18061152835175853No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11150.16345929716900226No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6990.10247358629697989No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG502.827027E-547.4964452
TAACGCA550.002581695634.5378044
GTAAGGT2450.031.0135384
GTACACG953.2383417E-530.3290861
TACCTGG5750.029.7369042
GGTATCA46300.029.0408081
GTACCTG6500.028.0738471
ATAACGC856.407291E-427.9390833
GTATCAA65200.027.8404251
GGTAAGG3050.026.4734293
TAAGGTG3300.024.4624865
GTACATG42950.024.1502861
TATCAAC76050.023.7326092
ATCAACG76650.023.6707653
TCAACGC77550.023.331394
GAGTACT43600.023.25289212-13
CAACGCA78100.023.1653825
GTATAGG2303.1905074E-922.9665891
AACGCAG79250.022.8891496
TACATGG44200.022.6736412
Produced by FastQC (version 0.11.5)