FastQCFastQC Report
Thu 26 May 2016
SRR936637_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936637_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715964
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48470.6769893458330307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40070.5596650110899432No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23830.3328379639199736No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11850.16551111508399866No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8090.1129945081037594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTACG902.3997234E-531.6897374
TACCTGG7950.031.087472
GTATCAA62000.030.586541
TGTACGG650.005823849229.2500215
GGTATCA42150.028.3025361
GTACCTG8750.028.2451881
GTATTAG2155.638867E-1126.5272351
TATCAAC74900.025.1282672
ATCAACG75250.025.011393
TCAACGC76200.024.6406424
CAACGCA76650.024.556285
GTACATG45100.024.343591
AACGCAG77800.024.1322066
TACTAGC1601.8484352E-623.7639792
GAGTACT39950.023.61526912-13
TACATGG46300.023.3020442
ACATGGG45400.023.031173
ACGCAGA83050.022.5510357
AGTACTT41800.022.45639412-13
CGCAGAG84000.022.2944368
Produced by FastQC (version 0.11.5)