##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936637_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 715964 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.41510606678548 31.0 31.0 34.0 30.0 34.0 2 31.438987155778783 31.0 31.0 34.0 28.0 34.0 3 31.46024241442307 31.0 31.0 34.0 28.0 34.0 4 35.10811856462057 37.0 35.0 37.0 32.0 37.0 5 35.09051153409948 37.0 35.0 37.0 32.0 37.0 6 35.229315999128445 37.0 35.0 37.0 32.0 37.0 7 35.09995474632803 37.0 35.0 37.0 32.0 37.0 8 35.05380158778933 37.0 35.0 37.0 32.0 37.0 9 36.519107105943874 39.0 37.0 39.0 32.0 39.0 10-11 36.42675190372701 39.0 37.0 39.0 32.0 39.0 12-13 36.325876021699415 39.0 37.0 39.0 32.0 39.0 14-15 37.35801730254593 40.0 37.0 41.0 32.0 41.0 16-17 37.244878932460296 39.0 37.0 41.0 32.0 41.0 18-19 37.248886117179076 39.5 37.0 41.0 31.0 41.0 20-21 37.19467948109123 39.5 36.5 41.0 31.0 41.0 22-23 37.06914523635267 39.0 36.0 41.0 31.0 41.0 24-25 37.17351570749367 39.0 36.0 41.0 31.0 41.0 26-27 37.14991466051366 39.0 36.0 41.0 31.0 41.0 28-29 37.048402573313744 39.0 36.0 41.0 31.0 41.0 30-31 36.8233388829606 39.0 36.0 41.0 30.0 41.0 32-33 36.59692456603963 39.0 35.0 41.0 30.0 41.0 34-35 36.35233475426139 39.0 35.0 40.5 30.0 41.0 36-37 36.135890910716185 39.0 35.0 40.0 30.0 41.0 38-39 35.93127377912856 38.0 35.0 40.0 29.0 41.0 40-41 35.73477716756709 38.0 35.0 40.0 28.5 41.0 42-43 35.45713052611584 38.0 35.0 40.0 27.0 41.0 44-45 35.114778117335504 38.0 34.5 40.0 26.0 41.0 46-47 34.689832868691724 38.0 34.0 40.0 24.0 41.0 48-49 34.485709477012804 38.0 34.0 40.0 23.0 41.0 50-51 33.54870147102368 37.5 33.0 39.5 22.0 40.5 52-53 33.685373147253216 37.5 33.0 39.5 22.0 40.5 54-55 34.08548544340218 38.0 34.0 40.0 22.0 41.0 56-57 33.99425739282981 38.0 34.0 40.0 21.5 41.0 58-59 33.742038007497584 38.0 33.0 40.0 19.5 41.0 60-61 33.43895293618115 37.5 33.0 40.0 18.5 41.0 62-63 33.11738998050181 37.0 33.0 40.0 18.0 41.0 64-65 32.77303328100295 36.0 32.0 39.5 15.5 41.0 66-67 32.39147568872178 36.0 32.0 39.0 12.5 41.0 68-69 31.992924225240376 35.5 31.0 39.0 9.0 40.5 70-71 31.559406618209856 35.0 31.0 38.5 7.5 40.0 72-73 31.179316976831238 35.0 31.0 37.5 6.5 40.0 74-75 30.75647867769888 35.0 30.5 37.0 2.0 39.0 76-77 30.321183746668826 35.0 30.0 36.5 2.0 39.0 78-79 29.88564788173707 34.0 29.5 36.0 2.0 38.5 80-81 29.417626444905054 34.0 29.0 35.5 2.0 37.5 82-83 28.954928599762 34.0 29.0 35.0 2.0 37.0 84-85 28.62980191741484 34.0 29.0 35.0 2.0 36.5 86-87 28.313179154259153 34.0 28.5 35.0 2.0 36.0 88-89 27.911906883586326 34.0 27.0 35.0 2.0 36.0 90-91 27.506396271320902 34.0 26.5 35.0 2.0 35.0 92-93 27.15501547563844 33.5 26.0 35.0 2.0 35.0 94-95 26.712924113502915 33.0 25.0 35.0 2.0 35.0 96-97 26.148420171963956 33.0 24.5 35.0 2.0 35.0 98-99 25.470245710678192 33.0 21.0 35.0 2.0 35.0 100-101 23.059486231151286 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35939130364700844 1101 2 0.326820336648705 1101 3 0.47228323187174936 1101 4 0.4321179524163128 1101 5 0.3287698495329465 1101 6 0.21955777442469326 1101 7 0.25821143271467406 1101 8 0.49570273382672525 1101 9 0.7089874637898532 1101 10-11 0.562091400336854 1101 12-13 0.66080828501849 1101 14-15 0.9532345781771241 1101 16-17 0.7883651595466219 1101 18-19 0.9758360151874186 1101 20-21 0.9187607841884713 1101 22-23 0.857371265977946 1101 24-25 0.8663020332058124 1101 26-27 0.7323728179782165 1101 28-29 0.7901301738468689 1101 30-31 0.9881968962018846 1101 32-33 0.8840632825529369 1101 34-35 0.6309126578961681 1101 36-37 0.6708720681884941 1101 38-39 0.7630365907605281 1101 40-41 0.567232820185545 1101 42-43 0.703034373046755 1101 44-45 0.7246850946955732 1101 46-47 0.7992662248008244 1101 48-49 0.8468889588550184 1101 50-51 0.846812285860473 1101 52-53 0.9929855191104053 1101 54-55 1.0152541333693392 1101 56-57 0.902788168475638 1101 58-59 0.9296266961743598 1101 60-61 0.7529401212390212 1101 62-63 0.7160386319458283 1101 64-65 0.603293679539334 1101 66-67 0.791347591141438 1101 68-69 0.7675481632927692 1101 70-71 0.5330918448000723 1101 72-73 0.8104500544856386 1101 74-75 0.7793414356992479 1101 76-77 0.9267182237263079 1101 78-79 0.6216025803628966 1101 80-81 0.6063140077946727 1101 82-83 0.6363414693916134 1101 84-85 0.8153136798125722 1101 86-87 0.5693784741724954 1101 88-89 0.3677813835228747 1101 90-91 0.2818036976043068 1101 92-93 0.5865334421931472 1101 94-95 0.5517053716788602 1101 96-97 0.5186116095862197 1101 98-99 0.5655883816812022 1101 100-101 1.0994992421839136 1102 1 0.3984659376454083 1102 2 0.5908010003683657 1102 3 0.42646984903361584 1102 4 0.49527333192757084 1102 5 0.5699265340593271 1102 6 0.33856917952160615 1102 7 0.36457246238997953 1102 8 0.3104457285607154 1102 9 0.4816000672950125 1102 10-11 0.5616135074767641 1102 12-13 0.4799913179839166 1102 14-15 0.3188329551794524 1102 16-17 0.5442273885492099 1102 18-19 0.5075182440061994 1102 20-21 0.6279078373356697 1102 22-23 0.5074790216276455 1102 24-25 0.19817794648664488 1102 26-27 0.35739234725375013 1102 28-29 0.4602117832429755 1102 30-31 0.447983820318008 1102 32-33 0.32470462396143773 1102 34-35 0.1609498324465335 1102 36-37 0.482901642404336 1102 38-39 0.3767130572942392 1102 40-41 0.16938127025033367 1102 42-43 0.4194807801195779 1102 44-45 0.07188689414600447 1102 46-47 0.2179899751569465 1102 48-49 0.6540833142051241 1102 50-51 0.5494687268452978 1102 52-53 0.48354598634258394 1102 54-55 0.6746118729361044 1102 56-57 0.7852575419764776 1102 58-59 0.7542285287096746 1102 60-61 0.6476083992623813 1102 62-63 0.774710319550941 1102 64-65 0.7956800191557392 1102 66-67 0.9932837464933826 1102 68-69 0.9115294703297501 1102 70-71 0.9113865484605448 1102 72-73 0.917550899069365 1102 74-75 0.69035341978174 1102 76-77 0.8448032518944153 1102 78-79 0.8737324201768821 1102 80-81 0.8862213701017936 1102 82-83 0.9691134317851215 1102 84-85 0.8764035512410082 1102 86-87 0.8495822219553482 1102 88-89 0.8468437025898794 1102 90-91 0.971665016772409 1102 92-93 0.6662416058898337 1102 94-95 0.9076114841123442 1102 96-97 0.6343547329836703 1102 98-99 0.7972113334048352 1102 100-101 0.9582577776261054 1103 1 0.30361501786392253 1103 2 0.44517423212409213 1103 3 0.332108914010778 1103 4 0.25901351750930246 1103 5 0.4120878379841528 1103 6 0.3369378600727444 1103 7 0.2731467245271162 1103 8 0.2766108675585528 1103 9 0.5520766010297535 1103 10-11 0.29793633676182907 1103 12-13 0.4486576171620129 1103 14-15 0.3752453951386556 1103 16-17 0.49329783092534996 1103 18-19 0.6267724071098755 1103 20-21 0.6869692573963064 1103 22-23 0.5913565207265918 1103 24-25 0.5784261426124644 1103 26-27 0.3048868290359721 1103 28-29 0.1679117288263896 1103 30-31 0.4067102604138384 1103 32-33 0.4306214254916938 1103 34-35 0.34914225982631564 1103 36-37 0.30507486615069723 1103 38-39 0.583994866845643 1103 40-41 0.364068351378144 1103 42-43 0.33878700410385676 1103 44-45 0.4075293984392516 1103 46-47 0.48737205255098104 1103 48-49 0.7277200140635429 1103 50-51 0.6050819969517534 1103 52-53 0.569047148878532 1103 54-55 0.7334483240330414 1103 56-57 0.7558095757665839 1103 58-59 0.4925548774045083 1103 60-61 0.5465243079838729 1103 62-63 0.6665968315426056 1103 64-65 0.7565701659862611 1103 66-67 0.6120337906626787 1103 68-69 0.6806359312970436 1103 70-71 0.7024940317998905 1103 72-73 0.7687886995089386 1103 74-75 0.5057663850611362 1103 76-77 0.6784769832932547 1103 78-79 0.7653026799865934 1103 80-81 0.69516441644069 1103 82-83 0.4896927916483058 1103 84-85 0.608310039534377 1103 86-87 0.7163328691828106 1103 88-89 0.8110065000259397 1103 90-91 0.9558406291944657 1103 92-93 0.8327981437705994 1103 94-95 0.6245463467634949 1103 96-97 0.7595941305851 1103 98-99 0.8877972656597279 1103 100-101 1.4254888690435976 1104 1 0.35985862131834523 1104 2 0.28290992206939336 1104 3 0.39857165784613713 1104 4 0.2822423212259082 1104 5 0.30085789965930587 1104 6 0.09753552262645826 1104 7 0.3887426046726219 1104 8 0.36976373685244823 1104 9 0.5492707455313166 1104 10-11 0.6249200804323607 1104 12-13 0.36446470309708445 1104 14-15 0.6388961504580877 1104 16-17 0.4554686124634415 1104 18-19 0.7580803114194694 1104 20-21 0.7573916306440793 1104 22-23 0.41239698714257145 1104 24-25 0.528892443597968 1104 26-27 0.1858890407554057 1104 28-29 0.44965871885640496 1104 30-31 0.3887136872373631 1104 32-33 0.2451608933757683 1104 34-35 0.22157166304538833 1104 36-37 0.24001888320048437 1104 38-39 0.45941219951968293 1104 40-41 0.7235206729169121 1104 42-43 0.5985471264575537 1104 44-45 0.730294776022312 1104 46-47 0.8815851672422426 1104 48-49 0.8470129538104771 1104 50-51 0.8416094824529168 1104 52-53 1.0292649476619005 1104 54-55 1.1472813449657977 1104 56-57 1.1611656165971453 1104 58-59 1.2750704111878477 1104 60-61 1.301701916337045 1104 62-63 1.1427202278543689 1104 64-65 1.0385969749389332 1104 66-67 1.1791558338774522 1104 68-69 1.1798731372476077 1104 70-71 1.2138496969831358 1104 72-73 0.9484956945986944 1104 74-75 0.934481556229823 1104 76-77 0.9738201064557437 1104 78-79 0.8793039755865344 1104 80-81 1.0602763631754328 1104 82-83 1.0263299048369738 1104 84-85 1.1845856113424311 1104 86-87 1.104261557522026 1104 88-89 1.1897682437961379 1104 90-91 1.1073184947510555 1104 92-93 1.0198858420874721 1104 94-95 1.061834326432539 1104 96-97 0.9791868215615125 1104 98-99 1.1826938284867445 1104 100-101 1.5662742019322877 1105 1 0.10600468611114522 1105 2 0.26956200588906043 1105 3 0.16428208169975633 1105 4 0.23585388144643815 1105 5 0.30874301218643296 1105 6 0.3561966097690572 1105 7 0.10126886676615499 1105 8 0.2536341281972696 1105 9 0.3937631270024511 1105 10-11 0.29363007487013704 1105 12-13 0.20315564761021676 1105 14-15 0.2838304844168107 1105 16-17 0.24562908142012674 1105 18-19 0.30741702243989266 1105 20-21 0.4080524699359245 1105 22-23 -0.08759220541152501 1105 24-25 0.1861004336147687 1105 26-27 -0.03735316419411561 1105 28-29 0.12084722088676614 1105 30-31 0.005216513024215885 1105 32-33 -0.03308040374830057 1105 34-35 0.05219376845112578 1105 36-37 -0.04555994919188322 1105 38-39 0.059003533965466204 1105 40-41 -0.1239819424849955 1105 42-43 0.26551640160936785 1105 44-45 -0.0222730731230385 1105 46-47 0.016954911581301246 1105 48-49 -0.09657289909874578 1105 50-51 0.2826555855761228 1105 52-53 0.2633837661630949 1105 54-55 0.03395374678374452 1105 56-57 0.15246202016734856 1105 58-59 0.18059316014096538 1105 60-61 -0.023822677086840827 1105 62-63 -0.1491715939351721 1105 64-65 0.08605174938033144 1105 66-67 0.15880757474083396 1105 68-69 0.2102924949682965 1105 70-71 0.3269914070957327 1105 72-73 0.10321941305175741 1105 74-75 0.15866256797967893 1105 76-77 0.21212861148336515 1105 78-79 0.5306298330492929 1105 80-81 0.2945162250706872 1105 82-83 0.21206378805315396 1105 84-85 0.2865913292018085 1105 86-87 0.12303985501787196 1105 88-89 -0.08877566181307373 1105 90-91 -0.014498712432004623 1105 92-93 -0.24124294520181877 1105 94-95 -0.25378776456327046 1105 96-97 -0.0019304089656273504 1105 98-99 0.40596341229920796 1105 100-101 0.3866335567133561 1106 1 0.2814026842710149 1106 2 0.10391763455422165 1106 3 0.26906890214442214 1106 4 0.35139803686011106 1106 5 0.4583048192328363 1106 6 0.2985081744075515 1106 7 0.32082227859937973 1106 8 0.2553379537503204 1106 9 0.48661387498187025 1106 10-11 0.2874173073307986 1106 12-13 0.5245281255611616 1106 14-15 0.612146051496417 1106 16-17 0.619971172416804 1106 18-19 0.53423337792605 1106 20-21 0.5239552031818988 1106 22-23 0.4033860382850918 1106 24-25 0.3479839972743406 1106 26-27 0.09745428802906275 1106 28-29 0.37960256083093924 1106 30-31 0.341025040206425 1106 32-33 0.2256795829928393 1106 34-35 0.3461633102459629 1106 36-37 -0.006447859155542801 1106 38-39 0.4538790462748281 1106 40-41 0.215627664612839 1106 42-43 -0.18247354891773426 1106 44-45 0.08078436438528769 1106 46-47 0.18782692949934443 1106 48-49 0.40184641546772326 1106 50-51 0.12469856535016532 1106 52-53 0.4121269624778776 1106 54-55 0.7274533425124119 1106 56-57 0.6408295384370035 1106 58-59 0.7655613915390589 1106 60-61 0.7465465573998316 1106 62-63 0.969961437666143 1106 64-65 0.9411599732816001 1106 66-67 0.7721863227698194 1106 68-69 0.755258000245508 1106 70-71 0.7969057201597387 1106 72-73 0.8796031517406782 1106 74-75 0.8973437450443278 1106 76-77 0.912126752764582 1106 78-79 0.9876485100752213 1106 80-81 0.9563231165803678 1106 82-83 1.018375997047496 1106 84-85 0.7812374446344386 1106 86-87 1.2974999746574056 1106 88-89 1.3801465600993659 1106 90-91 1.3546552297556467 1106 92-93 1.3826553766849052 1106 94-95 1.1674753587658202 1106 96-97 1.3868175270820515 1106 98-99 1.3574144520974727 1106 100-101 1.9421697506149016 1107 1 0.31077479266493313 1107 2 0.5352020584361341 1107 3 0.13272756411108233 1107 4 0.4374448900583232 1107 5 0.4665885821762146 1107 6 0.5684721899763261 1107 7 0.4699887616720062 1107 8 0.5453871569521951 1107 9 0.5873085948173724 1107 10-11 0.6900919052598553 1107 12-13 0.5204611504565193 1107 14-15 0.467088402968848 1107 16-17 0.5077908096349901 1107 18-19 0.7614368735565122 1107 20-21 0.7204346039309542 1107 22-23 0.7747519779193652 1107 24-25 0.6511770647635018 1107 26-27 0.5359047543579223 1107 28-29 0.5782417618296947 1107 30-31 0.5492786937277643 1107 32-33 0.3435697203211774 1107 34-35 0.5618025846823329 1107 36-37 0.6257420505161875 1107 38-39 0.6628876384757518 1107 40-41 0.5746766475018745 1107 42-43 0.7499770642863481 1107 44-45 0.7808666145138048 1107 46-47 0.5956105822863051 1107 48-49 0.6231234955881604 1107 50-51 0.5891349316569858 1107 52-53 0.8353119518294179 1107 54-55 0.7706126286987995 1107 56-57 0.7453973580742286 1107 58-59 0.7669177842834287 1107 60-61 0.4847906640312445 1107 62-63 0.6568932141220429 1107 64-65 0.8277142803977 1107 66-67 0.835532140497655 1107 68-69 0.7562303142647515 1107 70-71 0.8231890424774271 1107 72-73 0.7130150633217944 1107 74-75 0.7413497669287352 1107 76-77 0.8053675547033343 1107 78-79 0.8962377734959439 1107 80-81 1.0348169111019203 1107 82-83 1.040259671865737 1107 84-85 1.0813424604235244 1107 86-87 0.8451169240588889 1107 88-89 0.8269432113441333 1107 90-91 0.7564515138123404 1107 92-93 0.6355400061921408 1107 94-95 0.8157422310578042 1107 96-97 1.0100620009015415 1107 98-99 1.1770362483815333 1107 100-101 1.8803235289801812 1108 1 -0.08208795243310618 1108 2 -0.0035430396030804445 1108 3 0.0481001131306904 1108 4 0.3203468508426326 1108 5 0.3169415233526891 1108 6 0.05921728801553883 1108 7 -0.023187708916225347 1108 8 0.1233479412659193 1108 9 0.38040663403305786 1108 10-11 0.07758210133827959 1108 12-13 0.40528467986828076 1108 14-15 0.42234330492963323 1108 16-17 0.34402610375263265 1108 18-19 0.27477020688984766 1108 20-21 0.2157679372642889 1108 22-23 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-2.0583717634062424 2316 68-69 -2.773962231221912 2316 70-71 -2.659137401613723 2316 72-73 -1.8694014708594509 2316 74-75 -1.5625965323116837 2316 76-77 -1.694690738884372 2316 78-79 -2.199077541819374 2316 80-81 -1.9494703173029535 2316 82-83 -1.9156085492966035 2316 84-85 -2.095656303045825 2316 86-87 -2.60550134126526 2316 88-89 -2.897352849422518 2316 90-91 -2.6310383289716235 2316 92-93 -3.357922279623086 2316 94-95 -3.5276776594209096 2316 96-97 -3.9083287427833184 2316 98-99 -4.903880550770559 2316 100-101 -5.089243685292917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1662.0 3 634.0 4 602.0 5 683.0 6 799.0 7 935.0 8 1164.0 9 1447.0 10 1880.0 11 2422.0 12 3260.0 13 4200.0 14 4654.0 15 5919.0 16 7617.0 17 9968.0 18 8220.0 19 5175.0 20 4950.0 21 5354.0 22 5708.0 23 6455.0 24 7153.0 25 8263.0 26 9500.0 27 11323.0 28 13357.0 29 16043.0 30 19295.0 31 23339.0 32 29527.0 33 37542.0 34 50260.0 35 68580.0 36 95306.0 37 125468.0 38 101038.0 39 16255.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.1753656740712 18.481891990448954 14.853024801147916 21.489717534331938 2 15.545186571855659 20.201030999846328 40.8709014962071 23.38288093209092 3 19.106207844605507 25.86238691038329 30.408324066364536 24.623081178646668 4 12.421433302498205 16.97795241701764 37.79865126587054 32.80196301461362 5 13.002800478484946 37.36053505349417 34.2935639302843 15.343100537736587 6 30.239440634484144 36.179066270952454 18.344539646875365 15.236953447688034 7 27.677505904053884 33.71501236707145 21.01759338187001 17.589888347004653 8 26.727701065586828 33.44008763913691 20.117737144070805 19.714474151205458 9 27.165392837681118 16.660984504464224 20.864759882071844 35.30886277578281 10-11 24.752732587874604 26.748478945588595 27.160847486522215 21.33794098001459 12-13 26.397223926101777 24.53013677766957 27.81683892539616 21.2558003708325 14-15 23.202122620749204 25.322996874471688 27.310299153718148 24.16458135106096 16-17 21.361804934043445 28.801575607428383 27.778604519880584 22.058014938647585 18-19 21.20985998155446 26.51134680416981 31.337651267432438 20.9411419468433 20-21 22.3504539444874 25.45078409228214 31.287702116871063 20.9110598463594 22-23 21.885396534249935 25.52786367220869 31.424067188808568 21.162672604732805 24-25 21.59186224765949 25.808729296816345 31.55453278811603 21.04487566740813 26-27 21.323133463660344 25.881016885938724 31.513574333540767 21.282275316860165 28-29 21.074789101953904 26.041290551474212 31.63851317139399 21.2454071751779 30-31 21.18794759654536 25.7323227737681 31.665006767638815 21.414722862047725 32-33 20.977121670196993 26.065051586062786 31.51655970930505 21.44126703443517 34-35 21.702235839786116 25.951888901537984 31.128340262786082 21.21753499588982 36-37 21.671902522290218 25.702580119765834 31.19052042402748 21.434996933916473 38-39 21.440873883375122 25.880623284466083 31.28428448703353 21.394218345125264 40-41 21.439929939388353 26.03654018463541 31.29786026008745 21.225669615888787 42-43 21.41680383881824 26.134734363247684 30.801124939678008 21.647336858256065 44-45 21.47688409678656 26.262088019146685 30.591741142669182 21.669286741397574 46-47 21.797228329036063 26.631240938714285 29.904058617963624 21.667472114286035 48-49 21.78656479806692 26.84826560328512 29.437045624375834 21.928123974272125 50-51 21.728239007765797 27.022668864182357 29.420288842952118 21.828803285099728 52-53 22.0591419101348 27.129755467993782 29.098847806866228 21.71225481500519 54-55 21.63308493326722 27.003834254066472 29.191105089291327 22.17197572337498 56-57 21.728999542500326 27.38488295342234 28.90112768431824 21.984989819759097 58-59 22.0675672183541 26.958371659511283 28.91361785849731 22.06044326363731 60-61 21.798791986433592 26.854275680764815 29.57782384346111 21.769108489340482 62-63 21.6743583798235 27.045893267702347 29.482401714153216 21.797346638320935 64-65 21.54392056572756 27.164671917403556 29.280353873035338 22.01105364383355 66-67 21.500790937566567 27.346744933983775 29.03282129001393 22.119642838435723 68-69 21.474270771199354 27.26465686678522 29.235715413230817 22.02535694878461 70-71 21.50044244048355 27.23729266931642 28.99511185328436 22.26715303691567 72-73 21.657429470937753 27.183754082011774 28.90106237062572 22.257754076424757 74-75 21.497033254369104 27.64004045054306 28.68363931971573 22.1792869753721 76-77 21.620290861192455 27.404130555790303 28.808988858213024 22.16658972480422 78-79 21.669475408897227 27.46174674666635 28.63115196610792 22.237625878328497 80-81 21.627231767135623 27.71103439172101 28.521570169489628 22.140163671653738 82-83 21.649550056046568 27.688471866717418 28.55965554930876 22.102322527927253 84-85 21.543087432075655 27.48662466648973 28.489390531800467 22.48089736963415 86-87 21.708501441827885 27.4218273675624 28.46396617553495 22.405705015074766 88-89 21.610349121521722 27.333710001948525 28.376416261189185 22.67952461534056 90-91 21.489086656549368 27.27997144994207 28.721651601296482 22.50929029221208 92-93 21.58284737632106 27.26472032414363 28.72004196377621 22.432390335759102 94-95 21.28686109873348 27.190294134491417 28.827610675837445 22.695234090937664 96-97 21.352115076238846 26.980003017147453 28.85657095604463 22.811310950569066 98-99 21.205510874925434 27.289434090366164 28.51194642235978 22.993108612348617 100-101 21.126214487505134 27.299597336440634 28.61740534891454 22.95678282713969 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 201.0 1 157.5 2 269.0 3 392.0 4 288.5 5 248.5 6 389.0 7 797.5 8 1347.5 9 1613.5 10 1424.0 11 1198.5 12 1211.5 13 1304.5 14 1422.0 15 1478.0 16 1545.5 17 1637.0 18 1724.5 19 1819.0 20 1976.5 21 2236.5 22 2529.0 23 2869.5 24 3302.0 25 3919.5 26 4813.0 27 5789.5 28 6689.5 29 7829.0 30 9400.0 31 11347.0 32 13333.0 33 14607.5 34 15899.5 35 17116.0 36 18327.0 37 19814.0 38 20246.5 39 20227.5 40 20513.5 41 21241.5 42 22177.0 43 23179.5 44 24080.0 45 24880.5 46 25220.0 47 25673.5 48 28127.5 49 27889.5 50 25248.5 51 23965.5 52 22519.0 53 21948.5 54 21289.0 55 19764.0 56 18243.0 57 16324.5 58 14817.0 59 13385.5 60 11261.5 61 9217.5 62 7734.5 63 6170.0 64 4466.5 65 3404.5 66 2594.0 67 1975.5 68 1498.0 69 1061.0 70 809.5 71 588.0 72 478.5 73 444.5 74 355.5 75 273.5 76 184.5 77 108.0 78 67.0 79 43.0 80 30.5 81 20.0 82 12.5 83 8.5 84 6.5 85 4.5 86 3.0 87 3.0 88 2.5 89 3.5 90 2.5 91 0.0 92 0.5 93 1.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0322641920543491 2 0.02150946136956607 3 0.022347492332016695 4 0.04609170293478443 5 0.05195791967193881 6 0.041342860814230886 7 0.04944382678458693 8 0.04218089177668151 9 0.03827008061857859 10-11 0.04776776485968568 12-13 0.0386890960998039 14-15 0.03421959763340056 16-17 0.04239039951729417 18-19 0.048047108513835896 20-21 0.010126207462945064 22-23 0.018227173433301117 24-25 0.00579638082361683 26-27 0.010126207462945064 28-29 0.005237693515316413 30-31 0.007961294143280947 32-33 0.0034219597633400566 34-35 0.008170801883893604 36-37 0.00935801241403199 38-39 0.011034074338933242 40-41 0.001745897838438804 42-43 0.002025241492589013 44-45 0.0024442569738143257 46-47 8.38030962450626E-4 48-49 0.001257046443675939 50-51 5.586873083004173E-4 52-53 0.003491795676877608 54-55 0.006843919526680113 56-57 0.016271767854249653 58-59 0.009707191981719751 60-61 0.010894402511858137 62-63 0.005866216737154381 64-65 1.3967182707510432E-4 66-67 0.00579638082361683 68-69 0.014665541842885954 70-71 0.0067740836131425595 72-73 0.002514092887351878 74-75 0.0039108111581029215 76-77 0.00775178640266829 78-79 0.0062153963048421435 80-81 0.011103910252470794 82-83 0.00579638082361683 84-85 0.013129151745059806 86-87 0.004539334379940891 88-89 0.005377365342391517 90-91 0.003980647071640473 92-93 0.015154393237648821 94-95 0.00935801241403199 96-97 0.007821622316205843 98-99 0.01997307127173992 100-101 0.032124520227273995 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 715964.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.06202088522434 #Duplication Level Percentage of deduplicated Percentage of total 1 85.71111234729258 57.47960406329946 2 8.680547282053482 11.642700862484961 3 2.3064330007034095 4.640221741906283 4 0.9740534398852255 2.612879685156305 5 0.527615830233565 1.769149191324916 6 0.35606772297889017 1.4327172644978767 7 0.23975415689109963 1.1254878793725196 8 0.1815626636434669 0.9740767312988112 9 0.14077911931115475 0.8496839015503336 >10 0.8007766692753209 9.92955586341367 >50 0.053644325849590856 2.454377897238378 >100 0.0259900769578183 2.988108479721594 >500 8.31682462173147E-4 0.36936534577724356 >1k 8.31682462173147E-4 1.7320710929576895 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4847 0.6769893458330307 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4007 0.5596650110899432 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2383 0.3328379639199736 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1185 0.16551111508399866 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 809 0.1129945081037594 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.3967182707510432E-4 0.0 8 0.0 0.0 0.0 1.3967182707510432E-4 0.0 9 0.0 0.0 0.0 1.3967182707510432E-4 0.0 10-11 0.0 0.0 0.0 1.3967182707510432E-4 0.0 12-13 0.0 0.0 0.0 1.3967182707510432E-4 0.0 14-15 0.0 0.0 0.0 1.3967182707510432E-4 0.0 16-17 0.0 0.0 0.0 1.3967182707510432E-4 0.0 18-19 0.0 0.0 0.0 2.7934365415020865E-4 0.0 20-21 0.0 0.0 0.0 2.7934365415020865E-4 0.0 22-23 0.0 0.0 0.0 3.4917956768776087E-4 0.0 24-25 1.3967182707510432E-4 0.0 0.0 5.586873083004173E-4 0.0 26-27 1.3967182707510432E-4 0.0 0.0 8.380309624506261E-4 0.0 28-29 1.3967182707510432E-4 0.0 0.0 0.0018157337519763565 0.0 30-31 1.3967182707510432E-4 0.0 0.0 0.003771139331027817 0.0 32-33 1.3967182707510432E-4 0.0 0.0 0.007542278662055634 0.0 34-35 1.3967182707510432E-4 0.0 0.0 0.010545222944170377 0.0 36-37 2.7934365415020865E-4 0.0 0.0 0.014106854534585538 0.0 38-39 2.7934365415020865E-4 0.0 0.0 0.020392086752965236 0.0 40-41 2.7934365415020865E-4 0.0 0.0 0.025629780268281645 0.0 42-43 2.7934365415020865E-4 0.0 0.0 0.03833991653211614 0.0 44-45 2.7934365415020865E-4 0.0 0.0 0.051818247844863705 0.0 46-47 2.7934365415020865E-4 0.0 0.0 0.0645283841086982 0.0 48-49 2.7934365415020865E-4 0.0 0.0 0.08024146465464743 0.0 50-51 2.7934365415020865E-4 0.0 0.0 0.09874798174209876 0.0 52-53 2.7934365415020865E-4 0.0 0.0 0.13052332240168502 0.0 54-55 2.7934365415020865E-4 0.0 0.0 0.1558039231022789 0.0 56-57 2.7934365415020865E-4 0.0 0.0 0.20357168796196456 0.0 58-59 2.7934365415020865E-4 0.0 0.0 0.2711030163527775 0.0 60-61 2.7934365415020865E-4 0.0 0.0 0.3345140258448749 0.0 62-63 2.7934365415020865E-4 0.0 0.0 0.4236246515187915 0.0 64-65 2.7934365415020865E-4 1.3967182707510432E-4 0.0 0.4849405836047622 0.0 66-67 2.7934365415020865E-4 1.3967182707510432E-4 0.0 0.5894849461704779 0.0 68-69 2.7934365415020865E-4 1.3967182707510432E-4 0.0 0.7094630456279925 0.0 70-71 4.1901548122531303E-4 1.3967182707510432E-4 0.0 0.7955707270197943 0.0 72-73 4.1901548122531303E-4 1.3967182707510432E-4 0.0 0.959196272438279 1.3967182707510432E-4 74-75 4.1901548122531303E-4 1.3967182707510432E-4 0.0 1.1121369230855183 1.3967182707510432E-4 76-77 4.1901548122531303E-4 1.3967182707510432E-4 0.0 1.2120022794442178 1.3967182707510432E-4 78-79 4.1901548122531303E-4 1.3967182707510432E-4 0.0 1.3196892581191233 1.3967182707510432E-4 80-81 5.586873083004173E-4 1.3967182707510432E-4 0.0 1.5027291875010476 1.3967182707510432E-4 82-83 5.586873083004173E-4 1.3967182707510432E-4 0.0 1.66984652859641 1.3967182707510432E-4 84-85 6.285232218379694E-4 1.3967182707510432E-4 0.0 1.924957120749088 1.3967182707510432E-4 86-87 6.983591353755216E-4 1.3967182707510432E-4 0.0 2.3003949919269684 1.3967182707510432E-4 88-89 6.983591353755216E-4 1.3967182707510432E-4 0.0 2.7889670430356834 1.3967182707510432E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTACG 90 2.3997234E-5 31.689737 4 TACCTGG 795 0.0 31.08747 2 GTATCAA 6200 0.0 30.58654 1 TGTACGG 65 0.0058238492 29.250021 5 GGTATCA 4215 0.0 28.302536 1 GTACCTG 875 0.0 28.245188 1 GTATTAG 215 5.638867E-11 26.527235 1 TATCAAC 7490 0.0 25.128267 2 ATCAACG 7525 0.0 25.01139 3 TCAACGC 7620 0.0 24.640642 4 CAACGCA 7665 0.0 24.55628 5 GTACATG 4510 0.0 24.34359 1 AACGCAG 7780 0.0 24.132206 6 TACTAGC 160 1.8484352E-6 23.763979 2 GAGTACT 3995 0.0 23.615269 12-13 TACATGG 4630 0.0 23.302044 2 ACATGGG 4540 0.0 23.03117 3 ACGCAGA 8305 0.0 22.551035 7 AGTACTT 4180 0.0 22.456394 12-13 CGCAGAG 8400 0.0 22.294436 8 >>END_MODULE