FastQCFastQC Report
Thu 26 May 2016
SRR936637_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936637_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715964
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49090.6856489991116872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43520.6078517914308541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27040.3776726204110821No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14040.1960992452134465No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11880.16593013056522396No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7550.10545222944170377No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG702.0541494E-433.9241075
TACCTGG7250.033.411732
GTACCTG8100.030.5004651
GGTATCA49600.029.3108141
GTATCAA70650.028.0421941
GAGTACT46700.023.7501912-13
CCACGTC1401.780078E-523.7468749
ATCAACG84850.023.117173
TATCAAC85050.023.064422
TCAACGC85300.023.0508944
CAACGCA86800.022.7619825
AACGCAG88150.022.4672646
GTACATG48300.022.1321451
GTACTTT50800.021.78659214-15
TACATGG49600.021.0672472
AGTACTT48900.020.83605812-13
ACCTGGG11350.020.503913
GTAAGGT3252.1827873E-1120.4588454
ACCGTGC1404.8535867E-420.3544648
ACATGGG47750.020.2905223
Produced by FastQC (version 0.11.5)