##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936637_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 715964 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.886034772698068 31.0 31.0 34.0 30.0 34.0 2 32.02216871239336 31.0 31.0 34.0 30.0 34.0 3 32.109206608153485 33.0 31.0 34.0 30.0 34.0 4 35.867504511400014 37.0 35.0 37.0 35.0 37.0 5 35.64656463174126 37.0 35.0 37.0 33.0 37.0 6 35.76544491063797 37.0 35.0 37.0 33.0 37.0 7 35.707942019431144 37.0 35.0 37.0 33.0 37.0 8 35.69055706711511 37.0 35.0 37.0 33.0 37.0 9 37.31736092876178 39.0 37.0 39.0 34.0 39.0 10-11 37.19030286438983 39.0 37.0 39.0 33.0 39.0 12-13 37.089290802330844 39.0 37.0 39.0 33.0 39.0 14-15 38.16134191104581 40.0 37.5 41.0 33.0 41.0 16-17 38.09719343430675 40.0 37.0 41.0 33.0 41.0 18-19 38.279106212044184 40.0 38.0 41.0 33.5 41.0 20-21 38.32303998525066 40.0 38.0 41.0 34.0 41.0 22-23 38.22860227609209 40.0 38.0 41.0 33.0 41.0 24-25 38.10259594057802 40.0 37.0 41.0 33.0 41.0 26-27 37.87902254861976 40.0 37.0 41.0 33.0 41.0 28-29 37.780338815918114 40.0 37.0 41.0 33.0 41.0 30-31 37.68512103960535 40.0 37.0 41.0 33.0 41.0 32-33 37.48298727310312 39.5 36.0 41.0 32.5 41.0 34-35 37.2871135978904 39.0 36.0 41.0 32.0 41.0 36-37 37.025825320826186 39.0 35.5 41.0 31.0 41.0 38-39 36.83784031040667 39.0 35.0 41.0 31.0 41.0 40-41 36.57895438876815 39.0 35.0 41.0 30.5 41.0 42-43 36.28207633344693 39.0 35.0 40.0 30.0 41.0 44-45 35.94878862624378 39.0 35.0 40.0 29.5 41.0 46-47 35.459138308630045 38.5 35.0 40.0 27.5 41.0 48-49 35.00218516573459 38.0 34.5 40.0 25.5 41.0 50-51 34.63445159253817 38.0 34.0 40.0 24.0 41.0 52-53 34.342969758256004 38.0 34.0 40.0 23.5 41.0 54-55 34.07109784849518 38.0 33.0 40.0 23.0 41.0 56-57 33.75663860194088 38.0 33.0 40.0 22.0 41.0 58-59 33.42054139593611 37.0 33.0 40.0 21.0 41.0 60-61 33.0718681386215 37.0 32.0 39.5 20.0 41.0 62-63 32.68198051857355 36.0 32.0 39.0 18.5 40.0 64-65 32.25912755389936 35.5 31.0 39.0 17.5 40.0 66-67 31.811299869825856 35.0 31.0 38.5 13.5 40.0 68-69 31.54030859093474 35.0 31.0 38.0 12.5 40.0 70-71 31.09249277896654 35.0 30.0 37.5 8.0 39.5 72-73 30.65340156767659 34.5 30.0 37.0 7.5 39.0 74-75 30.126711678240806 34.0 29.0 36.0 6.0 39.0 76-77 28.709801051449514 32.5 28.0 35.0 2.0 37.0 78-79 29.484804403573364 34.0 29.0 35.0 2.0 37.5 80-81 29.29136870010224 34.0 29.0 35.0 2.0 37.0 82-83 28.959269600147493 34.0 29.0 35.0 2.0 36.5 84-85 28.54693322569291 34.0 29.0 35.0 2.0 36.0 86-87 28.158274019364104 33.0 27.5 35.0 2.0 36.0 88-89 27.74093320334542 33.0 27.0 35.0 2.0 35.0 90-91 27.276492952159607 33.0 26.0 35.0 2.0 35.0 92-93 26.79379759317508 33.0 25.5 34.5 2.0 35.0 94-95 26.206992251007033 32.0 24.5 34.0 2.0 35.0 96-97 25.45310239062299 32.0 21.5 34.0 2.0 35.0 98-99 24.50452606555637 32.0 10.0 34.0 2.0 35.0 100-101 21.348815024219093 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27872816029468694 1101 2 0.3066347751717444 1101 3 0.3403706248040308 1101 4 0.17475141887904044 1101 5 0.07565422442384317 1101 6 0.20039284624220954 1101 7 0.21954665772675952 1101 8 0.2207012775114876 1101 9 0.2676050703460007 1101 10-11 0.32367138705658505 1101 12-13 0.2935589679196937 1101 14-15 0.4563338993047239 1101 16-17 0.41305988012575057 1101 18-19 0.4111466868955773 1101 20-21 0.30542039564807766 1101 22-23 0.404789382305502 1101 24-25 0.3805626508608668 1101 26-27 0.1909375846633239 1101 28-29 0.3462401542008635 1101 30-31 0.3874108690991491 1101 32-33 0.40228650559315327 1101 34-35 0.27296198499046653 1101 36-37 0.28597827236188067 1101 38-39 0.39063677646322503 1101 40-41 0.3103819956270826 1101 42-43 0.42818352189564024 1101 44-45 0.47233226086332536 1101 46-47 0.36686186802955945 1101 48-49 0.13956115135924563 1101 50-51 0.4474157662067668 1101 52-53 0.5913382847673532 1101 54-55 0.534032974673643 1101 56-57 0.43024149055025873 1101 58-59 0.22261523193282784 1101 60-61 0.44213277100326565 1101 62-63 0.3532782626101536 1101 64-65 0.34744309824606745 1101 66-67 0.4172350626645489 1101 68-69 0.30489108900772166 1101 70-71 0.5553062000613842 1101 72-73 0.5985680252286976 1101 74-75 0.3804967176766674 1101 76-77 0.31760410462756283 1101 78-79 0.23108791681688245 1101 80-81 0.4453191443474438 1101 82-83 0.5344823030222194 1101 84-85 0.4622760565126427 1101 86-87 0.6383667634801853 1101 88-89 0.35589775783091326 1101 90-91 0.35629764419412524 1101 92-93 0.2521337416170546 1101 94-95 0.44868782568687493 1101 96-97 0.6387279941943973 1101 98-99 0.9397939021457375 1101 100-101 1.4881892815951723 1102 1 0.18204216347451663 1102 2 0.19783603239757497 1102 3 0.22594745067641497 1102 4 0.26999224930978727 1102 5 0.19189936508058025 1102 6 0.21655159857423456 1102 7 0.1779470032800532 1102 8 0.05925850058635973 1102 9 0.24283505628492463 1102 10-11 0.17006903986742827 1102 12-13 0.22321336866961872 1102 14-15 0.4995280616596389 1102 16-17 0.2658757719013991 1102 18-19 0.2137447698099848 1102 20-21 0.05038299134152879 1102 22-23 0.2326905136249522 1102 24-25 0.23235612762331925 1102 26-27 0.03130413767726026 1102 28-29 -0.08332119529504922 1102 30-31 0.27380792454390956 1102 32-33 0.19974264513786721 1102 34-35 0.08713633317217528 1102 36-37 0.10979318997130605 1102 38-39 0.0786830217876826 1102 40-41 0.2127539984576785 1102 42-43 0.1933727031365038 1102 44-45 0.400300251231954 1102 46-47 0.1692474264749393 1102 48-49 0.3009878453515853 1102 50-51 0.07260761261935755 1102 52-53 0.028428744371623793 1102 54-55 0.19346078987614845 1102 56-57 -0.05615694343160271 1102 58-59 0.112086425102639 1102 60-61 0.24036663522655033 1102 62-63 0.1150486718518664 1102 64-65 0.3686932475875935 1102 66-67 0.06716705483445295 1102 68-69 0.15642494128368511 1102 70-71 0.04751057278105009 1102 72-73 0.06164505788164476 1102 74-75 0.17766221565021922 1102 76-77 -0.0635453196034419 1102 78-79 -0.2800610019505214 1102 80-81 0.14296690054856498 1102 82-83 -0.06073440300933797 1102 84-85 0.24069689631744495 1102 86-87 0.5414016128979817 1102 88-89 0.26418023846246896 1102 90-91 0.6450839341831873 1102 92-93 0.6428260888981221 1102 94-95 0.774372958623804 1102 96-97 0.8355417354521748 1102 98-99 0.4741895514826595 1102 100-101 0.9666415979970076 1103 1 0.03505851436013785 1103 2 0.14503664110941372 1103 3 0.18857669004574973 1103 4 0.15221923003783644 1103 5 -0.022903592731260858 1103 6 0.11891742402188044 1103 7 0.09888866918112171 1103 8 0.16080784181598773 1103 9 0.26656515948796056 1103 10-11 -0.015060393846823672 1103 12-13 0.16548645036750997 1103 14-15 0.0765775760329177 1103 16-17 0.2933178630055906 1103 18-19 0.03908287107193331 1103 20-21 0.17489977920875077 1103 22-23 0.09539878659357015 1103 24-25 -0.09893540624098307 1103 26-27 0.23012664305414177 1103 28-29 0.30984681529616154 1103 30-31 0.2457268509119288 1103 32-33 0.22987022963079085 1103 34-35 0.24293264085433464 1103 36-37 0.13460750223765672 1103 38-39 0.24728724837736848 1103 40-41 0.259086415425287 1103 42-43 0.11476548481295623 1103 44-45 0.24620004463741907 1103 46-47 0.1766272329780776 1103 48-49 0.1451891819734641 1103 50-51 0.20408724880567064 1103 52-53 0.33249491115960694 1103 54-55 0.230497041681204 1103 56-57 0.3085754612863809 1103 58-59 0.5743093069197229 1103 60-61 0.30288091663327776 1103 62-63 0.3011044830004366 1103 64-65 0.32516823127439665 1103 66-67 0.391195978069387 1103 68-69 0.5702100028733561 1103 70-71 0.4395007353947449 1103 72-73 0.34784150407872616 1103 74-75 0.21921551444904708 1103 76-77 0.10190109655851742 1103 78-79 0.00749114726868072 1103 80-81 0.38928553669483534 1103 82-83 0.42691644987037947 1103 84-85 0.30769053358266873 1103 86-87 0.1900748849361058 1103 88-89 0.21606043718897894 1103 90-91 0.2992530448844093 1103 92-93 0.17520689657203903 1103 94-95 0.41520706344756064 1103 96-97 0.5144415312133361 1103 98-99 0.7060824945242885 1103 100-101 0.7722307222199234 1104 1 0.07526427709669292 1104 2 0.21713409475721335 1104 3 0.14865074623185137 1104 4 -0.0034137548634376458 1104 5 -0.10360260339888328 1104 6 0.08508999946757712 1104 7 0.10435472101278265 1104 8 0.11902666021350683 1104 9 0.15993881741124483 1104 10-11 0.10734505227269864 1104 12-13 0.24878432719351906 1104 14-15 0.2438613775837979 1104 16-17 0.2614146726865485 1104 18-19 0.1106302697142425 1104 20-21 0.19133818263096458 1104 22-23 0.10633663996079434 1104 24-25 0.1922161827855149 1104 26-27 0.2176114542530314 1104 28-29 0.1499168850209074 1104 30-31 0.1310030091897758 1104 32-33 0.11424560096583747 1104 34-35 0.17850337562816065 1104 36-37 0.40882238017144346 1104 38-39 0.40567817457800004 1104 40-41 0.45184715491387095 1104 42-43 0.43006774674646664 1104 44-45 0.4132327757850902 1104 46-47 0.47406516492458195 1104 48-49 0.44510715286463665 1104 50-51 0.4532391674818612 1104 52-53 0.6254749060232072 1104 54-55 0.6563929413757066 1104 56-57 0.508072937166375 1104 58-59 0.5261037895038783 1104 60-61 0.638079070595019 1104 62-63 0.5817666955631537 1104 64-65 0.6835741067051444 1104 66-67 0.38466239788210643 1104 68-69 0.6976809381201896 1104 70-71 0.5703812450484982 1104 72-73 0.33637851108953143 1104 74-75 0.6373301482120759 1104 76-77 0.7244234533238085 1104 78-79 0.8700713077237481 1104 80-81 0.5511186189577657 1104 82-83 0.5102085769925004 1104 84-85 0.22174204326325508 1104 86-87 0.5257924288159295 1104 88-89 0.7860113347177595 1104 90-91 0.6556158588661525 1104 92-93 0.9656225235005813 1104 94-95 0.9138539664653891 1104 96-97 0.8596882510072668 1104 98-99 1.0153379845332182 1104 100-101 1.2782084976978219 1105 1 0.0010574715759616993 1105 2 -0.02098852220096603 1105 3 0.035021260061057546 1105 4 -0.007830397753728846 1105 5 -0.11801397225556798 1105 6 -0.24602564596749943 1105 7 -0.03999817235663272 1105 8 -0.03163634056750908 1105 9 -0.17840340684757905 1105 10-11 -0.16606891901696486 1105 12-13 -0.14406416970690827 1105 14-15 -0.12757540750364882 1105 16-17 0.05339151305049228 1105 18-19 -0.02865032904708187 1105 20-21 0.08508657952649656 1105 22-23 0.06811646779097202 1105 24-25 -0.0800226588868469 1105 26-27 -0.08430895683338946 1105 28-29 -0.19873636312093623 1105 30-31 0.012255116641718189 1105 32-33 0.07857166304957275 1105 34-35 0.0668242731937525 1105 36-37 -0.217020960856793 1105 38-39 -0.10628278604542629 1105 40-41 -0.23784801747209627 1105 42-43 -0.48653335474841697 1105 44-45 -0.23237306081208686 1105 46-47 -0.1616381692445259 1105 48-49 -0.3023365259323043 1105 50-51 -0.3082152841771588 1105 52-53 -0.3078885440593595 1105 54-55 -0.45362397587961567 1105 56-57 -0.5311026132208241 1105 58-59 -0.6319306098759156 1105 60-61 -0.4005186416848332 1105 62-63 -0.2150770979368204 1105 64-65 -0.5724096158403427 1105 66-67 -0.25931367587367404 1105 68-69 -0.19257161027555014 1105 70-71 -0.3154006232274682 1105 72-73 -0.2660458151742908 1105 74-75 -0.636640046293131 1105 76-77 -0.7145368127410983 1105 78-79 -0.44257873565013384 1105 80-81 -0.28047399508961135 1105 82-83 -0.3386673045348374 1105 84-85 -0.3526607372764268 1105 86-87 -0.20949503483502951 1105 88-89 -0.2268041346280043 1105 90-91 0.0011327862084193896 1105 92-93 -0.08858474313784015 1105 94-95 -0.04444442279287486 1105 96-97 0.17977741964088878 1105 98-99 0.1123636313228289 1105 100-101 0.4266617360409093 1106 1 0.0901629012033176 1106 2 0.1461861942669742 1106 3 0.07578451195153235 1106 4 -0.04378576371996701 1106 5 -0.12725335355004574 1106 6 0.07174866220187681 1106 7 0.02908730702243645 1106 8 0.014814590805492855 1106 9 0.14570138771752994 1106 10-11 0.07982718479867401 1106 12-13 0.005210310393962914 1106 14-15 0.2256981060634473 1106 16-17 0.08226361151302086 1106 18-19 0.10210434175832717 1106 20-21 0.09496257200488145 1106 22-23 0.11919948126978142 1106 24-25 0.11786661103939622 1106 26-27 0.22701172482074838 1106 28-29 0.18178906570097553 1106 30-31 0.19813164195282695 1106 32-33 0.19289293639585026 1106 34-35 0.42652740482511575 1106 36-37 0.5730878671280735 1106 38-39 0.6714043791915714 1106 40-41 0.5642371552183718 1106 42-43 0.3895086894705031 1106 44-45 0.5346946244324755 1106 46-47 0.48541204404344995 1106 48-49 0.47776232692841347 1106 50-51 0.6198732094242061 1106 52-53 0.8236940310716037 1106 54-55 0.4843257953430893 1106 56-57 0.7865423590997551 1106 58-59 0.6137772986627184 1106 60-61 0.9180340049758229 1106 62-63 0.8357023248070803 1106 64-65 0.6695150255043743 1106 66-67 0.5400322100071939 1106 68-69 0.6334972304725248 1106 70-71 0.5414648643919158 1106 72-73 0.4934948334566087 1106 74-75 0.5491063687030113 1106 76-77 0.22094783269280427 1106 78-79 0.39969890737053504 1106 80-81 0.7593056847770576 1106 82-83 0.8760209108782782 1106 84-85 0.15771710927394622 1106 86-87 0.5928449372222317 1106 88-89 0.8331170866741537 1106 90-91 0.7912803375013091 1106 92-93 0.7965960017746916 1106 94-95 1.0695500258336885 1106 96-97 0.7754642626999697 1106 98-99 0.9969441649609934 1106 100-101 0.9276058102905296 1107 1 -0.0677500017855941 1107 2 -0.03296633565885543 1107 3 -0.14701639867488936 1107 4 0.10045452415548084 1107 5 -0.11198273593970498 1107 6 0.0865301852980096 1107 7 0.15603744288202392 1107 8 0.035159747594804 1107 9 0.09177320970474767 1107 10-11 0.057257602658708606 1107 12-13 0.15098764754604588 1107 14-15 0.08094889551780682 1107 16-17 0.07514016288263292 1107 18-19 0.05974552557267998 1107 20-21 0.19648199625398632 1107 22-23 0.05050006142693775 1107 24-25 0.06517512245894608 1107 26-27 0.10211784217404585 1107 28-29 -0.014792413332585852 1107 30-31 -0.012223061726395201 1107 32-33 0.010774261210421798 1107 34-35 0.0959119976135483 1107 36-37 0.08107420773173857 1107 38-39 0.13176275475153432 1107 40-41 0.3686955528096192 1107 42-43 -0.0802335445017377 1107 44-45 -0.11896917816721952 1107 46-47 0.020329426726860333 1107 48-49 -0.43299628363191545 1107 50-51 -0.5003449002915943 1107 52-53 -0.4180053153336232 1107 54-55 -0.23788320810848518 1107 56-57 -0.38370953191882506 1107 58-59 -0.5284552048255691 1107 60-61 -0.5744173426044057 1107 62-63 -0.4417053273923699 1107 64-65 -0.3399136298528944 1107 66-67 -0.3927598881054628 1107 68-69 -0.43821166282262425 1107 70-71 -0.328192916323367 1107 72-73 -0.4115165493736761 1107 74-75 -0.25203925808703076 1107 76-77 -0.269177011252264 1107 78-79 -0.37898655932550795 1107 80-81 -0.14729635678862962 1107 82-83 -0.1840723548924501 1107 84-85 -0.39930940170497564 1107 86-87 -0.35259385252587805 1107 88-89 -0.5038308460569922 1107 90-91 -0.501093166207287 1107 92-93 -0.45630478400778784 1107 94-95 0.13070373076135056 1107 96-97 0.014862808635196956 1107 98-99 0.4266797895479044 1107 100-101 0.7877739824282948 1108 1 -0.022965688060104128 1108 2 0.050955232968597386 1108 3 -0.07658502612586915 1108 4 -0.21978076996216345 1108 5 -0.24053175554754347 1108 6 -0.08946981470199233 1108 7 0.041841364450647234 1108 8 0.08959112014382242 1108 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-0.9914318515049096 2316 100-101 -1.2772816614508749 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 8.0 5 29.0 6 60.0 7 158.0 8 355.0 9 626.0 10 1114.0 11 1781.0 12 2598.0 13 3638.0 14 4384.0 15 5972.0 16 7853.0 17 10589.0 18 9137.0 19 5589.0 20 4711.0 21 4815.0 22 5041.0 23 5665.0 24 6449.0 25 7718.0 26 9312.0 27 11486.0 28 14033.0 29 17617.0 30 21141.0 31 26258.0 32 32089.0 33 41157.0 34 54468.0 35 73839.0 36 103583.0 37 135485.0 38 82459.0 39 4742.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.08224744094543 19.653650861960557 15.147674844277024 21.116426852816986 2 15.902313244059052 21.2903379004108 39.62406466635285 23.183284189177296 3 19.614101201359333 26.232681834625577 30.299788110439575 23.853428853575515 4 13.026249996508193 17.80862671482565 36.74077674513452 32.42434654353164 5 13.104922062685064 38.22476115984133 33.46178557461311 15.208531202860495 6 30.41409903291227 36.8775525026398 17.47643736277243 15.231911101675502 7 27.7815644362007 34.179092803548784 20.41010441865792 17.629238341592593 8 27.314362174634475 33.920979267113985 19.240492538730997 19.524166019520532 9 27.341344998854677 16.76834889294873 20.26269770768036 35.62760840051623 10-11 24.966531068317124 27.366517101105153 26.363810953785986 21.303140876791733 12-13 26.530941474796304 24.961642222676936 27.39014171950391 21.11727458302285 14-15 23.171976853164534 25.915111509027856 26.88012168430521 24.032789953502405 16-17 21.345448882203037 29.300730019785192 27.35175248746577 22.002068610546 18-19 21.18807649546625 26.775578101692265 31.289841388673175 20.74650401416831 20-21 22.32640188612835 25.62517109798817 31.426789615120317 20.621637400763166 22-23 21.878616248983 25.825526134764527 31.369609017358 20.926248598894478 24-25 21.75940733870889 26.09283797769978 31.45077148487968 20.696983198711656 26-27 21.494459661615434 26.181713042178643 31.376696916322942 20.947130379882978 28-29 21.1325248193764 26.48450076649358 31.450820444950395 20.932153969179627 30-31 21.22229834437803 26.259629627301734 31.37622572384139 21.141846304478847 32-33 21.180134113450315 26.393895756891787 31.23487173184014 21.191098397817758 34-35 21.643583995083144 26.27261997052682 31.197086205571967 20.88670982881807 36-37 21.91576741749607 25.999860310808454 31.015680111751355 21.068692159944124 38-39 21.58772573716187 26.27480341302506 31.132504405266342 21.004966444546724 40-41 21.642382052478233 26.429770627721894 31.02385069431835 20.903996625481525 42-43 21.666588682739736 26.583556751464293 30.39920554165424 21.35064902414173 44-45 21.758056192039867 26.630845073471743 30.202924623108558 21.408174111379825 46-47 22.11069419041613 27.124846448177877 29.336691046401107 21.427768315004887 48-49 22.286518403676194 27.21495340156364 28.88258032075926 21.615947874000902 50-51 22.1802114243453 27.50911895814463 28.777357747395605 21.533311870114467 52-53 22.430649303123356 27.785813006455108 28.415121563193797 21.36841612722774 54-55 21.837067206931255 27.70797497854236 28.62536324343124 21.829594571095146 56-57 22.070536605328968 27.971889275086614 28.32973324422153 21.62784087536289 58-59 22.291647170725778 27.503662909013197 28.33192031409532 21.872769606165697 60-61 22.056279580721107 27.63220840750702 28.86261276204058 21.448899249731298 62-63 21.96971178424465 27.67759616271046 28.855866979922396 21.49682507312249 64-65 21.68843078354313 27.83541731998595 28.625686290806406 21.850465605664514 66-67 21.747082864254367 27.938157001544816 28.413055806196592 21.90170432800422 68-69 21.855770325357977 27.808689202713378 28.488220640296003 21.847319831632642 70-71 21.941675838449978 27.921585442843494 28.35771072288551 21.779027995821018 72-73 21.956455546275063 27.822442098558852 28.285178739535 21.935923615631083 74-75 21.97701965035004 28.27866677096196 27.90557830806966 21.83873527061834 76-77 21.94290527186203 28.02315300454093 28.093830009232708 21.94011171436433 78-79 21.93736232884079 28.05900601597649 27.922888786147194 22.08074286903553 80-81 21.999201034743816 28.412294008039936 27.808251132100242 21.780253825116002 82-83 21.919326519751426 28.51284286159266 27.758641641629055 21.809188977026857 84-85 21.815727242124435 28.4677553403537 27.644894589735962 22.0716228277859 86-87 21.900364699950135 28.253979782967914 27.548953739955383 22.296701777126568 88-89 21.742807794143427 28.273779799174882 27.47834358737611 22.505068819305585 90-91 21.641138466911123 28.242849659507595 27.738781211803737 22.377230661777546 92-93 21.772846376034284 28.19740319237495 27.77998927308382 22.24976115850695 94-95 21.549719323583105 28.143275568511168 27.759239834122262 22.54776527378347 96-97 21.751415585830454 27.881593613033168 27.782635294249104 22.584355506887274 98-99 21.411715572924997 28.20742389947824 27.3851534581242 22.995707069472566 100-101 21.03997597530485 28.67123880826338 27.450449066249494 22.838336150182283 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 390.0 1 292.0 2 322.5 3 427.0 4 341.0 5 279.0 6 411.0 7 830.0 8 1256.0 9 1514.0 10 1450.0 11 1261.5 12 1264.5 13 1379.5 14 1530.0 15 1573.5 16 1687.0 17 1844.0 18 1950.0 19 2060.0 20 2193.0 21 2447.5 22 2690.5 23 2990.5 24 3376.0 25 3932.5 26 4804.0 27 5802.5 28 6771.5 29 8023.0 30 9548.5 31 11117.5 32 13020.0 33 14273.5 34 15608.5 35 16826.5 36 17990.0 37 19542.5 38 20114.5 39 20124.0 40 20268.5 41 21082.0 42 21885.0 43 22825.5 44 24027.0 45 24743.0 46 24822.5 47 25381.5 48 28196.5 49 28129.5 50 25426.0 51 23836.0 52 22102.0 53 21540.5 54 21248.5 55 20010.0 56 18156.0 57 16328.0 58 15163.5 59 13700.5 60 11550.5 61 9473.0 62 7841.5 63 6167.5 64 4402.5 65 3383.5 66 2686.0 67 2156.5 68 1619.0 69 1086.0 70 812.5 71 606.0 72 497.0 73 452.5 74 360.5 75 289.0 76 223.0 77 142.5 78 97.0 79 59.5 80 39.5 81 30.5 82 29.0 83 15.0 84 3.0 85 2.5 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.037711393310278174 2 0.006005888564229487 3 0.003491795676877608 4 2.7934365415020865E-4 5 5.586873083004173E-4 6 0.0 7 0.0 8 0.0 9 0.0011173746166008346 10-11 2.095077406126565E-4 12-13 0.0010475387030632825 14-15 0.0018157337519763565 16-17 0.0034219597633400566 18-19 0.0 20-21 0.0 22-23 0.0016062260113637 24-25 9.777027895257304E-4 26-27 0.004609170293478443 28-29 0.00579638082361683 30-31 0.0013268823572134911 32-33 4.19015481225313E-4 34-35 0.008240637797431156 36-37 0.012430792609684287 38-39 0.008310473710968708 40-41 0.0013967182707510435 42-43 6.285232218379695E-4 44-45 0.001187210530138387 46-47 6.983591353755216E-5 48-49 9.078668759881783E-4 50-51 0.0014665541842885954 52-53 0.002723600627964534 54-55 0.0025839288008894304 56-57 0.0013268823572134911 58-59 4.19015481225313E-4 60-61 0.002863272455039639 62-63 0.005866216737154381 64-65 0.009567520154644647 66-67 0.003072780195652295 68-69 0.004120318898715578 70-71 0.0 72-73 8.38030962450626E-4 74-75 0.006704247699605008 76-77 0.004329826639328235 78-79 0.005167857601778861 80-81 0.0053075294288539646 82-83 0.005726544910079278 84-85 0.006564575872529903 86-87 0.004609170293478443 88-89 0.009427848327569543 90-91 0.012430792609684287 92-93 0.0011173746166008346 94-95 0.002095077406126565 96-97 8.38030962450626E-4 98-99 0.002723600627964534 100-101 0.004748842120553548 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 715964.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.84174352922059 #Duplication Level Percentage of deduplicated Percentage of total 1 83.83562325768457 53.52212358629459 2 9.724666646501266 12.416793479061988 3 2.6707736592502607 5.115205409353594 4 1.1746996440994917 2.999794936098658 5 0.6092548991778406 1.9447947508616423 6 0.3840260739757439 1.4710136473975761 7 0.27870788029086485 1.2455237909171473 8 0.19930869272056567 1.017937155504847 9 0.1506520711932149 0.8656101802136795 >10 0.8766954674012873 10.505372827567355 >50 0.06117013831340357 2.734339608624714 >100 0.03200986707815872 3.607173443634859 >500 0.0013154739891087024 0.5167701816571436 >1k 0.0010962283242572522 2.0375470028123073 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4909 0.6856489991116872 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4352 0.6078517914308541 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2704 0.3776726204110821 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1404 0.1960992452134465 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1188 0.16593013056522396 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 755 0.10545222944170377 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.3967182707510432E-4 0.0 0.0 0.0 5 0.0 1.3967182707510432E-4 0.0 0.0 0.0 6 0.0 1.3967182707510432E-4 0.0 0.0 0.0 7 0.0 1.3967182707510432E-4 0.0 0.0 0.0 8 0.0 1.3967182707510432E-4 0.0 0.0 0.0 9 0.0 1.3967182707510432E-4 0.0 0.0 0.0 10-11 0.0 1.3967182707510432E-4 0.0 0.0 0.0 12-13 0.0 1.3967182707510432E-4 0.0 0.0 0.0 14-15 0.0 1.3967182707510432E-4 0.0 0.0 0.0 16-17 0.0 1.3967182707510432E-4 0.0 0.0 0.0 18-19 0.0 1.3967182707510432E-4 0.0 1.3967182707510432E-4 0.0 20-21 0.0 1.3967182707510432E-4 0.0 1.3967182707510432E-4 0.0 22-23 6.983591353755216E-5 1.3967182707510432E-4 0.0 2.7934365415020865E-4 0.0 24-25 4.1901548122531303E-4 1.3967182707510432E-4 0.0 5.586873083004173E-4 0.0 26-27 4.1901548122531303E-4 1.3967182707510432E-4 0.0 8.380309624506261E-4 0.0 28-29 4.1901548122531303E-4 1.3967182707510432E-4 0.0 0.0018157337519763565 0.0 30-31 4.1901548122531303E-4 1.3967182707510432E-4 0.0 0.0038409752445653688 0.0 32-33 4.1901548122531303E-4 1.3967182707510432E-4 0.0 0.007472442748518082 0.0 34-35 4.1901548122531303E-4 1.3967182707510432E-4 0.0 0.010405551117095273 0.0 36-37 5.586873083004173E-4 1.3967182707510432E-4 0.0 0.014316362275198193 0.0 38-39 6.983591353755216E-4 1.3967182707510432E-4 0.0 0.020811102234190547 0.0 40-41 6.983591353755216E-4 1.3967182707510432E-4 0.0 0.02611863166304451 0.0 42-43 8.38030962450626E-4 1.3967182707510432E-4 0.0 0.03910811158102921 0.0 44-45 9.777027895257304E-4 1.3967182707510432E-4 0.0 0.052446771066701675 0.0 46-47 9.777027895257304E-4 1.3967182707510432E-4 0.0 0.06501723550346107 0.0 48-49 9.777027895257304E-4 1.3967182707510432E-4 0.0 0.08038113648172254 0.0 50-51 9.777027895257304E-4 1.3967182707510432E-4 0.0 0.09902732539624898 0.0 52-53 9.777027895257304E-4 1.3967182707510432E-4 0.0 0.13094233788291032 0.0 54-55 9.777027895257304E-4 1.3967182707510432E-4 0.0 0.1558039231022789 0.0 56-57 9.777027895257304E-4 1.3967182707510432E-4 0.0 0.2032225083942768 0.0 58-59 9.777027895257304E-4 1.3967182707510432E-4 0.0 0.2715918677475404 0.0 60-61 9.777027895257304E-4 1.3967182707510432E-4 0.0 0.33507271315317533 0.0 62-63 9.777027895257304E-4 1.3967182707510432E-4 0.0 0.4244626824812421 0.0 64-65 9.777027895257304E-4 1.3967182707510432E-4 0.0 0.4852199272589125 0.0 66-67 9.777027895257304E-4 1.3967182707510432E-4 0.0 0.589764289824628 0.0 68-69 9.777027895257304E-4 1.3967182707510432E-4 0.0 0.7107899279852059 0.0 70-71 9.777027895257304E-4 1.3967182707510432E-4 0.0 0.7960595784145572 0.0 72-73 9.777027895257304E-4 1.3967182707510432E-4 0.0 0.9594057801788918 0.0 74-75 9.777027895257304E-4 1.3967182707510432E-4 0.0 1.1145113441457952 0.0 76-77 9.777027895257304E-4 1.3967182707510432E-4 0.0 1.2154242392075578 0.0 78-79 9.777027895257304E-4 1.3967182707510432E-4 0.0 1.3228318742283132 0.0 80-81 9.777027895257304E-4 1.3967182707510432E-4 0.0 1.50608131135085 0.0 82-83 9.777027895257304E-4 1.3967182707510432E-4 0.0 1.6738271756680503 0.0 84-85 9.777027895257304E-4 1.3967182707510432E-4 0.0 1.9274712136364398 0.0 86-87 9.777027895257304E-4 1.3967182707510432E-4 0.0 2.3018615461112573 0.0 88-89 9.777027895257304E-4 1.3967182707510432E-4 0.0 2.7900145817387467 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACCG 70 2.0541494E-4 33.924107 5 TACCTGG 725 0.0 33.41173 2 GTACCTG 810 0.0 30.500465 1 GGTATCA 4960 0.0 29.310814 1 GTATCAA 7065 0.0 28.042194 1 GAGTACT 4670 0.0 23.75019 12-13 CCACGTC 140 1.780078E-5 23.746874 9 ATCAACG 8485 0.0 23.11717 3 TATCAAC 8505 0.0 23.06442 2 TCAACGC 8530 0.0 23.050894 4 CAACGCA 8680 0.0 22.761982 5 AACGCAG 8815 0.0 22.467264 6 GTACATG 4830 0.0 22.132145 1 GTACTTT 5080 0.0 21.786592 14-15 TACATGG 4960 0.0 21.067247 2 AGTACTT 4890 0.0 20.836058 12-13 ACCTGGG 1135 0.0 20.50391 3 GTAAGGT 325 2.1827873E-11 20.458845 4 ACCGTGC 140 4.8535867E-4 20.354464 8 ACATGGG 4775 0.0 20.290522 3 >>END_MODULE